To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
The effects of DP on the clonal growth of erythroid and granulocyte-macrophage progenitors were investigated using clonogenic assays according to procedures previously tested in our laboratory. (bmj.com)
Real-time PCR1
This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)
CFUs4
It can be represented as a percentage or as colony forming units (CFUs) per microgram of DNA. (wikipedia.org)
Here is an example of how to calculate transformation efficiency using colony forming units (CFUs): Plate a known number of cells on agar plates containing the appropriate antibiotics. (wikipedia.org)
For example, if you plate 1x 107 cells and count 1000 colonies, the transformation efficiency is: (1000/1x 107) x 100 = 0.1% Alternatively, CFUs can be reported per microgram of DNA used for the transformation. (wikipedia.org)
Furthermore, the antimicrobial abilities evaluated in vitro with Streptococcus mutans ( S. mutans ), Fusobacterium nucleatum ( F. nucleatum ) and Candida albicans ( C. albicans ) using the live/dead, MTT and colony-forming units (CFUs) assay. (peerj.com)
Megakaryocytes1
In vitro, MPL515/630 but not MPL515/625 or MPL515/625/630 retained the ability to induce TPO-independent proliferation and increase colony-forming unit megakaryocytes (CFU-Mk). (biomedcentral.com)
Cytotoxicity2
Investigation of toxicity of Cu-Ti thin films was conducted the using the MTT assay, which revealed their biocompatibility and absence of cytotoxicity, further confirming their potential as promising biomaterials for various applications. (nature.com)
These strains produced and released Stx1a as measured by cytotoxicity and neutralization assays using anti-Stx/Stx1a antiserum. (cdc.gov)
Isolates1
Fungal isolates that displayed the highest ratio of clear zone/colony diameter on plates of phosphate solubilization medium, were selected and identified as Aspergillus niger and Penicillium sp. (scialert.net)
Phosphate1
On average, soil contains 0.02 to 0.5% total P. It is added to the soil in the form of phosphate fertilizers, a part of which (1%) is utilized by plants and the rest is rapidly converted into insoluble complexes e.g., calcium phosphate, iron phosphate and aluminum phosphate in the soil. (scialert.net)
Viable2
Antibacterial activity of the prepared sample was assessed by determining the number of colony-forming units per milliliter (CFU/ml) using a standard viable cell count assay. (nature.com)
Here we identify causes underlying hybrid infertility of two recently diverged fission yeast species Schizosaccharomyces pombe and S. kambucha , which mate to form viable hybrid diploids that efficiently complete meiosis, but generate few viable gametes. (elifesciences.org)
Predominant form1
Several cytochrome P-450 isozymes are involved in the metabolism of benzene, although the predominant form is CYP2E1 (Gut et al. (cdc.gov)
Metabolism2
In our 3-D culture assay, glucose uptake and metabolism determined the signaling activity and the morphology of both malignant and non-malignant mammary epithelial cells," Bissell says. (rdworldonline.com)
Catechol can also be formed from benzene dihydrodiol via metabolism by cytosolic dehydrogenases (Henderson et al. (cdc.gov)
Induce1
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Cell4
We found these pathways to be similarly active in normal and malignant fibroblasts, as we find also now in our breast cancer cell studies in 3-D assays. (rdworldonline.com)
After colony enumeration, BFU-E and CFU-mix plates were washed and total cells counted using a cell analyser (Coulter Electronics, Inc., Chicago, IL). (bmj.com)
Moreover, polymerized flagellin filaments can elicit a more robust immunoglobulin response than monomeric flagellin, and the multimeric antigen form can also promote T cell-independent antibody responses. (biomedcentral.com)
The effects on microglia were not mediated by astrocytes because pure microglial cells responded to various pneumococcal lysates similarly with distinct cell shape changes as seen in mixed cultures. (biomedcentral.com)
Methods1
Other TB diagnostic methods include blood cultures for extrapulmonary TB (ETB) and serologic tests by Enzyme-linked ImmunoSorbent Assay (ELISA), which do not present sufficient sensitivity and specificity to be useful and determination of adenosine deaminase. (scielo.org.mx)
Distinct1
Four distinct forms of botulism can occur, depending on the mode of acquisition of the toxin. (cdc.gov)
Escherichia2
The standard plasmids used for determination of transformation efficiency in Escherichia coli are pBR322 or other similarly sized or smaller vectors, such as the pUC series of vectors. (wikipedia.org)
The Escherichia coli dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. (asm.org)
Tissue1
Bissell says this demonstration of an active role in breast cancer development for glucose uptake could only have been revealed through a 3-D culture assay in which both malignant and non-malignant breast cells behave in a manner that is phenotypically analogous to their corresponding architecture in living tissue. (rdworldonline.com)
Results1
The Petri dishes were analyzed with a colony counter, and the results were expressed as colony-forming units. (bvsalud.org)
Mouse3
The study was carried out using a 3-D culture assay developed earlier by Bissell and her group for mouse mammary cells and later with her collaborator, Ole Petersen, for human breast cells. (rdworldonline.com)
MegaCult™-C Collagen and Medium with Lipids kit includes medium and collagen solution for colony-forming unit (CFU) assays of human or mouse megakaryocyte progenitor cells (CFU-Mk). (stemcell.com)
TNF-α-mediated bystander assays used a murine coculture system of primary colonic epithelial cells and E. faecalis -infected macrophages ( in vitro ), with an interleukin 10 (IL-10)-deficient mouse model of colon cancer that involves long-term colonization with E. faecalis ( in vivo ). (aacrjournals.org)
Culture1
This can be calculated by multiplying the number of colonies by the volume of the culture plated and dividing by the amount of DNA used. (wikipedia.org)
Plates1
Count the number of colonies that grow on the plates. (wikipedia.org)
Test1
La présente étude menée dans un hôpital de soins tertiaires en Égypte a utilisé la méthode de PCR en temps réel pour évaluer la présence du gène blaKPC dans les isolats de K. pneumoniae non sensibles à l'ertapénème, puis a comparé les résultats à l'aide du test de Hodge modifié. (who.int)
Formation1
Concentrations of 10 6 M to 10 9 M were associated with a dose dependent inhibition of colony formation, with the most clinically important effects seen on the earliest erythroid and granulocyte-macrophage colonies. (bmj.com)
Effects3
The concentrations of erythropoietin (Epo) used to promote the growth of erythroid colonies ranged from 0.2 U/ml to 20 U/ml, based on the possibility that very high Epo concentrations might mask the effects of DP on progenitors of preterm infants. (bmj.com)
The checkerboard assay is a well-established tool used to determine the antimicrobial effects of two compounds in combination. (mdpi.com)
While helpful in traditional checkerboard assays, these measurements become less useful in a polymicrobial context as they do not enable assessment of the drug effects on the individual members of the community. (mdpi.com)
Data1
Usually, data collected from the checkerboard assay use visible turbidity and optical density as a readout. (mdpi.com)
Capable2
Paired cysteine substitutions were made at amino acids predicted to form inter-monomer disulfide cross-links, and these substitutions were capable of forming flagella when transfected into a flagellin-negative strain of Salmonella enterica subspecies Typhimurium. (biomedcentral.com)
KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)
Found1
No difference in colony-forming units was found among the groups of endodontic absorbent paper points. (bvsalud.org)
Provide1
It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
Activity1
TRIC-B deficiency causes a dysregulation of calcium flux from the ER, which impacts on multiple collagen specific chaperones and modifying enzymatic activity, leading to a rare form of osteogenesis imperfecta (OI Type XIV). (bvsalud.org)
Quickly1
Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed. (oncotarget.com)
Response1
None of the tested forms induced a pro-inflammatory response in Caco-2 cells or in macrophages either. (springer.com)
Expression1
Similarly, the higher Rv3737 mRNA level resulted in lower C(t) value by Xpert MTB/RIF assay, demonstrating that a positive correlation between Rv3737 expression and bacterial load in TB patients. (biomedcentral.com)