Given the lack of effective treatments, we constructed a bifidobacterial recombinant thymidine kinase (BF-rTK) -ganciclovir (GCV) targeting system (BKV) to meet this requirement and to explore antitumor mechanisms. (biomedcentral.com)
The Herpes Simplex Virus thymidine kinase/ganciclovir (HSV-TK + GCV) system is currently one of the best-studied tumor suicide gene therapy systems [ 9 - 11 ]. (biomedcentral.com)
A colorectal cancer model was used to decipher the molecular mechanism of BF-rTK + GCV (bifidobacterial recombination thymidine kinase/ganciclovir) using a human apoptosis antibody array kit in a murine cancer model in vivo . (biomedcentral.com)
To investigate the in vitro effects of suicide gene therapy system of herpes simplex virus thymidine kinase gene (HSV-TK) in combination with the treatment of nucleotide analog-ganciclovir (GCV) on human pancreatic cancer, and to provide a novel clinical therapeutic method for human pancreatic cancer. (wjgnet.com)
Although the combination of herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) with ganciclovir (GCV) has been shown as a promising suicide gene treatment strategy for glioma, the almost immunodepressive dose of GCV required for its adequate in vivo efficacy has hampered its further clinical application. (hindawi.com)
Herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) in combination with ganciclovir (GCV) has been shown as one of the most promising suicide gene systems for tumor treatment in laboratory studies and is currently being tested in several clinical trials. (hindawi.com)
Biomarker2
Evaluation of serum thymidine kinase 1 activity as a biomarker for treatment effectiveness and prediction of relapse in dogs with non-Hodgkin lymphoma. (soladisclinicalstudies.fr)
Read more about the study Evaluation of serum thymidine kinase as biomarker for gastrointestinal lymphoma on this project page. (slu.se)
Herpes1
In addition to cellular TKs, virus specific thymidine kinases have been identified in Herpes simplex virus, Varicella zoster virus and Epstein-Barr virus. (wikipedia.org)
Serum TK1 activity measured by refined ELISA-based method (DiviTum assay, Biovica International) before treatment, at clinical response, and every 4 weeks until relapse or last follow-up. (soladisclinicalstudies.fr)
OPV IgG and IgM were detected by immunofluorescence assay ( 3 ) in serum samples up to 5 months after symptom onset ( Technical Appendix Table 1). (cdc.gov)
Automated CLIA assay for the quantitative determination of Thymidine Kinase (TK) activity in human serum or EDTA plasma. (diasorin.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Thymidine Kinase 1, Soluble (TK1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (diastrip.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Thymidine Kinase 1, Soluble (TK1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (diastrip.com)
After transfection, the cells had been cultured in 6H moderate with 5% serum for 12 hr, and had been put through luciferase assay. (bioshockinfinitereleasedate.com)
GAL4 cross assays The pG5-luc reporter plasmid consists of five GAL4-binding sites upstream from the coding series with a minor thymidine kinase area for luciferase assay. (bioshockinfinitereleasedate.com)
Inhibitors3
Although combination chemotherapy regimens, including the nucleoside analogue fludarabine, were once the most commonly used first-line therapy in CLL, non-chemotherapy regimens (eg, with Bruton tyrosine kinase [BTK] inhibitors) are currently preferred in most cases. (medscape.com)
They differ biochemically from thymidine kinase from mammalian cells and are inhibited by specific inhibitors that do not influence the activity of mammalian thymidine kinases. (wikipedia.org)
Constitutive activation of AKT promotes NED, and NED induced by androgen withdrawal is suppressed by the phosphoinositol-3-kinase (PI3K)/AKT inhibitors [ 5 , 6 ]. (oncotarget.com)
Tumor2
The thymidine kinase level in serum, therefore, serves as a measure of malignant proliferation and, indirectly, as a measure of the aggressivity of the tumor. (wikipedia.org)
Shao Y, Sun X, He Y, Liu C, Liu H. Elevated levels of serum tumor markers CEA and CA15-3 are prognostic parameters for different molecular subtypes of breast cancer. (scielo.br)
Induces1
Here, we show that bergenin, a phytochemical isolated from tender leaves of Shorea robusta , activates the MAP kinase and ERK pathways and induces TNF-α, NO and IL-12 production in infected macrophages. (frontiersin.org)
Lymphoma2
Prognosis of malignant lymphoma in dogs and correlation to thymidine kinase (TK1) : a retrospective study. (slu.se)
Periodically 'cleansed' of spontaneous mutants: Prior to dose-range finding and mutagenicity testing, the mouse lymphoma cells were grown for 1 day in R10-medium containing hypoxanthine, aminopterine and thymidine to reduce the amount of spontaneous mutants, followed by a recovery period of 2 days on R10-medium containing hypoxanthine and thymidine only. (europa.eu)
Hematological malignancies1
Determination of serum Thymidine Kinase activity is useful for prognosis and monitoring of patients with hematological malignancies. (diasorin.com)
Determination1
Determination of viral thymidine kinase has been suggested for confirmation of diagnosis and for control of treatment of viral infections. (wikipedia.org)
Inhibitor1
Cyclin-dependent kinase inhibitor 1b (CDKN1b) was down-regulated and Thymidine kinase 1 (TK1) was up-regulated in VSMCs. (bvsalud.org)
Antibody1
Serum levels of TNF-R did not correlate with clinical signs, however, lower anti-collagen antibody levels corresponded with lack of clinical symptoms. (ox.ac.uk)
Prognostic3
The identification of putative prognostic factors in canine mammary neoplasms (CMNs) has been focused on tissue-specific biomarkers, but the serum biomarkers, including cancer antigen 15-3 (CA 15-3), c-reactive protein (CRP), and lactate dehydrogenase (LDH) have been demonstrated to display clinical application in cases of CMNs. (scielo.br)
The aim of the study was to evaluate the levels of these serum biomarkers and their association with well-established prognostic factors in CMNs. (scielo.br)
Darlix A, Lamy PJ, Lopez-Crapez E, Braccini AL, Firmin N, Romieu G, Thezenas S, Jacot W. Serum HER2 extra-cellular domain, S100ß and CA 15-3 levels are independent prognostic factors in metastatic breast cancer patients. (scielo.br)
ELISA4
Human Elisa Laboratories manufactures the human thymidine kinase 1 (tk1) elisa kit reagents distributed by Genprice. (diastrip.com)
The Human Thymidine Kinase 1 (Tk1) Elisa Kit reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (diastrip.com)
Description: A competitive ELISA for quantitative measurement of Human Thymidine kinase, cytosolic(TK1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (diastrip.com)
Description: A sandwich ELISA kit for detection of Thymidine Kinase 1, Soluble from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (diastrip.com)
Albumin3
Then it is transmitted through the serous membrane of mucosa and binds to serum albumin. (dkart.in)
Copper can affect the absorption of zinc due to competition for the binding site on serum albumin. (dkart.in)
Phosphoinositide 3-kinases (PI3Ks) are a family of lipid-modifying enzymes that phosphorylate the 3′-OH group of inositol phospholipids and play key roles in physiology ranging from cell growth and metabolism to organismal development. (nature.com)
Deoxyuridine1
Thymidine monophosphate is produced by the cell in two different reactions - either by phosphorylation of thymidine as described above or by methylation of deoxyuridine monophosphate, a product of other metabolic pathways unrelated to thymidine, by the enzyme thymidylate synthase (De novo synthesis). (wikipedia.org)
Demonstrates1
Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. (hsanmartino.it)
Protein1
Nucleotide sequences of the hemagglutinin, thymidine kinase, and A-type inclusion body protein genes were identical to those of CPXV strains T2000 and E1989 previously identified in Finland ( 3 ). (cdc.gov)
Clinical1
Choline kinase (ChoK) is increased in human mammary tumors with high incidence, and this activation is associated with clinical variable indicators of greater malignancy. (aacrjournals.org)
Cells6
Cells were exposed to the test item in basic medium supplemented with 5%/10% (v/v) heat-inactivated horse serum (R5-medium) for 3/24 hour exposure. (europa.eu)
The CHL cells were cultured in Eagle's MEM supplemented with 10 % (v/v) heat-inactivated calf serum. (biomedcentral.com)
The L5178Y cells were cultured in RPMI1640 supplemented with 10 % (v/v) heat-inactivated horse serum and sodium pyruvate (200 μg/ml) in a humidified incubator at 37 °C with 5 % CO 2 . (biomedcentral.com)
A) After FRTL-5 cells had been expanded to near confluency in complet e 6H moderate with 5% serum, the cells had been starved for 6 times with 0H moderate and serum. (bioshockinfinitereleasedate.com)
A, B FRTL-5 thyroid cells had been harvested to near confluency in Coon's customized Ham's F-12 moderate formulated with 5% (v/v) leg serum. (bioshockinfinitereleasedate.com)
Unifying a blood marker of abnormal cell proliferation, Thymidine Kinase Type 1 (TK1) and inflammatory marker CRP or HPT (species dependent) into the Neoplasia Index-, the Cancer Panel is a Rule-in for neoplasia. (vdilab.com)
Significantly1
The levels of CA 15-3, CRP and LDH were significantly higher in the serum of female dogs with CMNs compared to the healthy ones. (scielo.br)
Phosphatidylinositol 3-kinase type 2α (PI3KC2α) is an essential member of the structurally unresolved class II PI3K family with crucial functions in lipid signaling, endocytosis, angiogenesis, viral replication, platelet formation and a role in mitosis. (nature.com)
It is present during cell division in much higher concentrations than TK2 and it is released in quantities that completely dominate the thymidine kinase activity in blood and other body fluids. (wikipedia.org)
Genes1
Expression of HCV E1E2 genes cloned from patient serum. (addgene.org)
Tissue1
The former was first found in fetal tissue, the second was found to be more abundant in adult tissue, and so initially they were termed fetal and adult thymidine kinases. (wikipedia.org)
Requirement1
When a cell prepares to divide, a complete new set-up of DNA is required, and the requirement for building blocks, including thymidine triphosphate, increases. (wikipedia.org)
Levels1
Female dogs with CMNs were found to exhibit highest serum levels of CA 15-3, CRP, and LDH. (scielo.br)
Amount2
Selective medium consisted of basic medium supplemented with 20% (v/v) heat-inactivated horse serum (total amount of serum = 20%, R20-medium) and 5 µg/mL trifluorothymidine (TFT) (Sigma). (europa.eu)
Non-selective medium consisted of basic medium supplemented with 20% (v/v) heat-inactivated horse serum (total amount of serum = 20%, R20-medium). (europa.eu)
System1
Complexes of Vps34, the sole class III PI3K member, produce PI 3-phosphate (PI(3)P) in the endolysosomal system and during autophagy to regulate vesicle-mediated sorting en route to lysosomes 1 . (nature.com)
Present1
Thymidine kinase is a salvage enzyme that is only present in anticipation of cell division. (wikipedia.org)