Sequencing seq. Medical search. Frequent questions

ChIP-seq, or chromatin immunoprecipitation and sequencing, is a technique that allows researchers to understand transcriptional regulation via mapping of protein-DNA interactions and epigenetic markers on a genome-wide scale. (news-medical.net)
Tag sequencing using high-throughput sequencing technologies are now regularly employed to identify specific sequence features such as transcription factor binding sites (ChIP-seq) or regions of open chromatin (DNase-seq). (unc.edu)
RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq) and DNA (genomic and amplicon-derived). (utmb.edu)
Can I use NGS data from other projects, such as exome-seq, RNA-seq, ChIP-seq, or ATAC-seq, for mtDNA sequence analysis? (northwestern.edu)
We combined global ChIP-seq of GATA1, GATA2, and PU.1 with expression profiling during differentiation to erythroid and neutrophil line- ages. (lu.se)
We performed chromatin immunoprecipitation sequencing (ChIP-seq) using a histone 3 lysine 9 acetylation (H3K9ac) mark in peripheral blood mononuclear cells from patients with atherosclerosis with T2D (n = 8) or without T2D (ND, n = 10). (lu.se)

Since messenger RNA (mRNA) is generated from a DNA gene, that information can be used to identify the original gene sequence and thus measure the activity of thousands of genes (i.e. the transcriptome) in these cells. (epfl.ch)
The critical advance leading up to Live-seq emerged when the researchers managed to preserve and read out the mRNA (the transcriptome), from these tiny amounts of cytoplasmic sample. (epfl.ch)
As a result, Live-seq can now connect a cell's transcriptome at a given time to its later molecular or phenotypic behavior, i.e. to monitor the activity of thousands of genes in a single cell at discrete time points - what the scientists refer to as a "temporal" transcriptomic analysis. (epfl.ch)
Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that "The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome. (seqanswers.com)
To explore the underlying molecular events that are associated with the progression of PGD, we sampled Atlantic salmon from three different marine production sites in Scotland and examined the gill tissue at three different levels of organization: gross morphology with the use of PGD scores (macroscopic examination), whole transcriptome (gene expression by RNA-seq) and histopathology (microscopic examination). (frontiersin.org)
Mild-Seq solves this downside by combining high-resolution barcode labeling with full-transcriptome sequencing by way of NGS, giving us one of the best of each worlds and extra key benefits. (nthenews.com)

It focuses on studying chromosomes at a molecular scale, involving techniques that analyze either the whole genome or particular DNA sequences to examine variations in structure and behavior at the chromosomal or subchromosomal level. (seqanswers.com)
Single-cell DNA genome sequencing involves isolating a single cell, amplifying the whole genome or region of interest, constructing sequencing libraries, and then applying next-generation DNA sequencing (for example Illumina, Ion Torrent, MGI). (wikipedia.org)
Some of the genome assembly tools used in single cell single-cell sequencing include SPAdes, IDBA-UD, Cortex, and HyDA. (wikipedia.org)
PELE-Seq is also well-suited for whole genome sequencing of mitochondria and viruses, and for high-throughput rare mutation screens. (umn.edu)
Genome coverage and nucleotide sequence identity of the full genome or several contigs in relation to the OvPV reference genome strain OvPV/Wales/2017/S014175 (International Nucleotide Sequence Data Collaboration accession no. (cdc.gov)
To intuitively summarize and display individual sequence data as an accurate and interpretable signal, we developed F-Seq, a software package that generates a continuous tag sequence density estimation allowing identification of biologically meaningful sites whose output can be displayed directly in the UCSC Genome Browser. (unc.edu)
Standard mtDNA-seq bioinformatics service includes sequencing data QC, alignment to the reference mitochondrial genome, and variant analysis. (northwestern.edu)
NUMTs are a result of insertion of mtDNA sequence into the nuclear genome. (northwestern.edu)
Here, we describe a simple, novel, high-throughput approach to Norovirus and Hepatitis C virus whole genome sequence determination based on RNA shotgun sequencing (also known as RNA-Seq). (ucl.ac.uk)

To do this, scientists have developed "single-cell RNA sequencing" (scRNA-seq), which provides unprecedented insights for basic and biomedical research, and even drug development. (epfl.ch)
Now, scientists from the groups of professors Bart Deplancke at EPFL and Julia Vorholt at ETH Zurich have addressed a problem with scRNA-seq: needing to lyse (kill) cells. (epfl.ch)
Live-seq can address a broad range of biological questions by transforming scRNA-seq from an end-point to a temporal and spatial analysis approach," says Julia Vorholt. (epfl.ch)
I have a large 10X scRNA-seq dataset spanning 5 brain regions collected from three donors. (bioconductor.org)
Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution. (rsc.org)

Examples of this kind of meta data are secondary structures (RNA and protein), protein hydrophobicity assignments, or other alternative alphabets for polypeptides, sequence quality data and nucleotide alignments with translations. (metacpan.org)
RAD-seq analysis of 94 Nassau grouper sampled from nine locations in The Bahamas generated a working dataset of 13,241 single nucleotide polymorphisms (SNPs). (frontiersin.org)
The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
The minor allele frequencies of rare Single Nucleotide Polymorphisms (SNPs) within a population have been difficult to track with Next-Generation Sequencing (NGS), due to the high error rate of standard methods such as Illumina sequencing. (umn.edu)
Compared with Sanger sequencing, our method had no nucleotide differences in 14,019 nucleotides (nt) for Noroviruses (from a total of 2 Norovirus genomes obtained with Sanger sequencing), and 8 variants in 9,542 nt for Hepatitis C virus (1 variant per 1,193 nt). (ucl.ac.uk)
We present an improved ddRAD-Seq protocol for identifying single nucleotide polymorphisms (SNPs). (sagescience.com)
This is the position of the first changed nucleotide at the mutated sequence compared to the selected reference sequence (see above). (lu.se)
The number indicates the first different nucleotide in the mutated sequence compared to the wild type sequence. (lu.se)
Set position number 10020 of altered nucleotide according to the DNA reference sequence or use systematic names (e.g. (lu.se)

We developed a modified high-throughput droplet barcoding technique for single-cell Drop-Seq via introduction of hydrodynamic ordering in a spiral microchannel. (rsc.org)
RT-PCR was performed as prescreening for sample selection for high-throughput sequencing. (cdc.gov)
Number (proportion) of OvPV reads in the complete high-throughput sequencing dataset. (cdc.gov)
FML -seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects . (bvsalud.org)

Accurate and comprehensive annotation of transcript sequences is essential for transcript quantification and differential gene and transcript expression analysis. (dundee.ac.uk)
In addition, due to the picogram level of the number of nucleic acids used, heavy amplification is often needed during sample preparation of single-cell sequencing, resulting in uneven coverage, noise, and inaccurate quantification of sequencing data. (wikipedia.org)

We have four core methods for the preparation of RNA-seq libraries. (nanoporetech.com)
PCR-cDNA libraries are created by reverse transcription, strand-switching & second-strand synthesis, followed by PCR & attachment of sequencing adapters (Fig. 1a). (nanoporetech.com)
cDNA libraries can also be sequenced without any amplification being performed. (nanoporetech.com)
We evaluated all four RNA-seq approaches by making libraries from the ERCC spike-in panel (Fig. 2). (nanoporetech.com)
We have prepared ATAC libraries for 24 samples following the protocol from Buenrostro et.al, using their custom Nextera-like primers and we have attempted to sequence these on NextSeq. (seqanswers.com)
We have successfully sequenced ATAC-Seq libraries on the Next-Seq. (seqanswers.com)
To test the specificity and sensitivity of the PELE-Seq method, we sequenced control E. coli DNA libraries containing known rare alleles present at frequencies ranging from 0.2-0.4% of the total reads. (umn.edu)
PELE-Seq had higher specificity and sensitivity than standard libraries. (umn.edu)
Costs are reduced by indexing ("bar coding") individual template libraries, allowing multiple libraries to be sequenced in each of the flow cell lanes. (utmb.edu)
We also offer expertise, consultation, and discussion around the design of experiments, the construction of sequencing libraries as well as support in data processing and bioinformatic strategies. (lu.se)

Nextera sequencing primers aren't included in HiSeq High Output chemstry reagents. (seqanswers.com)
As well as, as a result of the unique tissue setting is disrupted, many spatial transcriptomics and all single-cell sequencing strategies stop researchers from revisiting their samples to carry out follow-up evaluation, and they're pricey as a result of they require specialised devices or reagents . (nthenews.com)
Current methods for profiling DNA methylation require costly reagents , sequencing, and labor time . (bvsalud.org)

The challenge with RNA sequencing is to capture mRNA from a specific cell out of a mixed population - which is how most cells exist in nature. (epfl.ch)
However, accuracy is significantly affected by sequencing errors, mRNA degradation, or incomplete cDNA synthesis. (dundee.ac.uk)
We took the advantage of the translating mRNA sequencing (RNC-seq) result as a standard to evaluate the integration strategies of the protein identifications from various search engines. (nih.gov)
Background: Previously, using microarrays and mRNA-Sequencing (mRNA-Seq) we found that occupational exposure to a range of benzene levels perturbed gene expression in peripheral blood mononuclear cells. (cdc.gov)

Advancements in single-cell DNA sequencing have enabled collecting of genomic data from uncultivated prokaryotic species present in complex microbiomes. (wikipedia.org)
Total genomic DNA is the starting material for mtDNA-seq. (northwestern.edu)
By generating viral genomic sequences that are not biased by primer-specific amplification or enrichment, this method offers the prospect of large-scale, affordable studies of RNA viruses which could be adapted to routine diagnostic laboratory workflows in the near future, with the potential to directly characterize within-host viral diversity. (ucl.ac.uk)
What is the Value of Rapid Genomic Sequencing in Acute Infant Care? (cdc.gov)
All 10x Genomic services include a final report containing a Cell Ranger web summary file for quality control (QC) of the sequencing outcome, as well as FASTQ, BAM, gene count matrix containing all single cells or nuclei passing QC, basic visualization and clustering. (lu.se)

PCR-cDNA, Direct cDNA and Direct RNA sequencing give low-bias datasets with a high proportion of unambiguously mapping reads. (nanoporetech.com)
In the Direct cDNA protocol, sequencing adapters are attached directly to the double-stranded cDNA product, making library preparation considerably faster (Fig. 1c). (nanoporetech.com)
The cDNA protocols currently perform with the highest throughput, due to a different motor protein being used for Direct RNA sequencing. (nanoporetech.com)
To determine the number of permissible PCR cycles we calculated the variation in sequence counts which could be explained by transcript length, for a PCR-cDNA library created from 1 ng input. (nanoporetech.com)
In these situations, the authors typically have multiple sequencing runs of the same Cellranger-derived cDNA library. (bioconductor.org)
We offer services for DNA and cDNA (RNA-Seq) sequencing. (lu.se)

Here, we adopted a combined approach, integrating restriction-site-associated DNA sequencing (RAD-seq) and acoustic telemetry to establish country-wide demographic structure, diversity and connectivity, and the origins of Nassau grouper using an active fish spawning aggregation (FSA) in the central Bahamas. (frontiersin.org)

For consolidated targeted DNA and RNA sequencing, we provide multimodal products to streamline your workflow and enable enrichment and profiling of DNA variants, RNA fusions and gene expression levels. (qiagen.com)
Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins. (nanoporetech.com)
In contrast, integration of the gill RNA-seq data with the gill histopathology enabled us to identify common gene expression patterns associated with multifactorial gill disease, independently from the origin of samples. (frontiersin.org)
We describe isogenic colony sequencing (ICO-seq), a massively-parallel strategy to assess the gene expression profiles of large numbers of genetically distinct yeast colonies. (rsc.org)
Here, we applied Illumina RNA-Seq to study the gene expression patterns associated with resistance to three widely used insecticides (malathion, alphacypermethrin, and lambda-cyhalothrin) in Ae. (cdc.gov)

ATAC-seq(Assay for Transposase-Accessible Chromatin using sequencing)は、ゲノムワイドなクロマチンアクセシビリティの査定を可能にします。この技術は、トランスポゼー (diagenode.com)
The authors assessed two liquid biopsy assays, including a digital polymerase chain reaction test and a sequencing-based assay (HPV-seq) that enables highly sensitive detection and accurate HPV genotyping from baseline plasma cell-free DNA, the main cause of cervical cancer. (msdmanuals.com)

Usually, a mix of millions of cells is used in sequencing the DNA or RNA using traditional methods like Sanger sequencing or Illumina sequencing. (wikipedia.org)
Metagenomic sequencing was performed on the Illumina HiSeq 2500. (genomeweb.com)
The researchers collected phenotypic information on patients via questionnaires containing 160 clinical items, as well as whole-exome sequencing data generated using the Illumina NovaSeq 6000 instrument with a mean depth of 200x. (genomeweb.com)
We demonstrate the effectiveness of this method by sequencing three Norovirus samples from faeces and two Hepatitis C virus samples from blood, on an Illumina MiSeq benchtop sequencer. (ucl.ac.uk)
Our unit has instruments and resources for traditional Sanger Sequencing (ABI 3500) but also for the so-called Next-Generation Sequencing (NGS) based on the Illumina MiSeq platform. (lu.se)
Target sequence enrichment (48.48 Fluidigm Access Arrays) and sequencing were performed (Illumina Hi-Seq 2,500 instrument, v4 chemistry). (lu.se)

With Live-seq, we can now uniquely address highly interesting and biomedically relevant questions, such as why certain cells differentiate and sister cells do not, or why certain cells are resistant to a cancer drug, while their sister cells are again not," says Bart Deplancke. (epfl.ch)

For example, small and large RNAs from the same sample cannot be sequenced in a single sequence run. (rna-seqblog.com)
We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
In development, sequencing the RNAs expressed by individual cells can give insight into the existence and behavior of different cell types. (wikipedia.org)

Like typical next-generation sequencing experiments, single-cell sequencing protocols generally contain the following steps: isolation of a single cell, nucleic acid extraction and amplification, sequencing library preparation, sequencing, and bioinformatic data analysis. (wikipedia.org)
Multiple displacement amplification (MDA) is a widely used technique, enabling amplifying femtograms of DNA from bacterium to micrograms for sequencing. (wikipedia.org)

For example, in cancer, sequencing the DNA of individual cells can give information about mutations carried by small populations of cells. (wikipedia.org)
As each mitochondrion contains 2-10 copies of mtDNA and each cell contains hundreds to thousands copies, the presence of heterogeneous mtDNA sequences, due to different mutations in different copies, results in a condition called heteroplasmy. (northwestern.edu)
NUSeq provides mtDNA-seq for detection of specific mtDNA mutations associated with a disease, determination of mtDNA heteroplasmy, and haplogroup classification (Fig. 2). (northwestern.edu)

above, three of the samples were sequenced over both batches (B1 and B2), meaning that reads from the same test tube were sequenced twice. (bioconductor.org)
The issue I have is that, for those samples that were sequenced in both batches, I'm currently counting their reads as if they derived from cells from a different prep, when in fact they come from cells from the same prep. (bioconductor.org)

Sequence alignments illustrated that for homozygous loci, more than 90% of the SNPs from the resequencing data were discovered by our method. (sagescience.com)
Our analyses suggest that our ddRAD-Seq method is reliable in identifying SNPs suitable for phylogenetic and association genetic studies while reducing cost and time over known methods. (sagescience.com)

A Seq field in a header, footer, annotation, or footnote doesn't affect the sequence numbering that results from Seq fields in the document text. (microsoft.com)

Nanopore sequencing offers advantages in all areas of research. (nanoporetech.com)

Furthermore, this strategy also significantly improves the peptides coverage of the protein amino acid sequence. (nih.gov)

The researchers, led by Dr Wanze Chen (EPFL) and Dr Orane Guillaume Gentil (ETH Zurich), present Live-seq. (epfl.ch)
Testing Live-seq, the researchers showed that it can accurately identify ("stratify") diverse cell types and states without introducing major disturbances. (epfl.ch)
Mild-Seq permits researchers to "geotag" the total repertoire of RNA sequences with distinctive DNA barcodes unique to a couple cells of curiosity. (nthenews.com)
NEW YORK (GenomeWeb) - Researchers with Cincinnati Children's Hospital have developed a metagenomic sequencing protocol for fecal samples to detect bacteria resistant to multiple drugs, in order to prevent the spread of infection with such bugs. (genomeweb.com)
The researchers sequenced the fecal microbiomes of 17 inpatient samples, who were deemed at a higher risk to becoming infected with drug resistant bacteria due to prolonged hospital stays, as well as 11 outpatient samples, who were deemed at a lower risk. (genomeweb.com)
When comparing the sequencing method to culture, the researchers found that the culture missed several drug-resistance genes and was "cumbersome and required additional testing given the growth of isolates not intended to be selected on all types of screening media. (genomeweb.com)
The researchers noted that their next step would be to develop prediction models in order to better stratify patients according to their risk of developing a multi-drug resistance bacterial based on their metagenomic sequencing profile. (genomeweb.com)

Design and test primers for this sequence using Primer-BLAST. (nih.gov)

By contrast, metagenomic sequencing was a "single diagnostic test with precise and clear quantitative detection of clinically important species and antibiotic-resistance genes of MDR bacteria relative to the abundance of all other species and antibiotic-resistance genes within the gut microbiota. (genomeweb.com)

Uniform coverage and being able to deal with the large amounts of data and high number of variants that result from the analysis are prerequisites for reliable results in your digital DNA sequencing (DNA-seq) applications. (qiagen.com)
The length of the meta data sequence is not dependent on the amount of the meta information. (metacpan.org)
The heatmap represents the normalized gene level RNA-Seq expression data in FPKM (Fragments Per Kilobase Of Exon Per Million Fragments Mapped). (brain-map.org)
It improves the precision of differential gene and transcript expression, differential alternative splicing, and transcription start/end site usage analysis from RNA-seq data. (dundee.ac.uk)
Seq is a fundamental and widely-used data structure in Scala, representing ordered, indexed collections of elements. (sparkcodehub.com)
It is ideal for functional programming, stack-like data structures, and situations where elements need to be frequently added or removed from the head of the sequence. (sparkcodehub.com)
Be mindful of the performance implications of different Seq operations, especially when working with large data sets or nested sequences. (sparkcodehub.com)
Seq is a powerful and versatile data structure in Scala, providing a foundation for various concrete implementations, including List, Vector, and Array. (sparkcodehub.com)
The minimal amount of starting materials from a single cell makes degradation, sample loss, and contamination exert pronounced effects on the quality of sequencing data. (wikipedia.org)
As F-seq was created initially for DNase-seq data, no shift based on fragment length was actually necessary and a simple command line procedure was used if the user wanted to shift sequence based on strand. (unc.edu)
The benefits of using sequence numbers are that they are guaranteed to be unique, allow all other data of the object to change, are efficient values for querying and indexes, and can be efficiently assigned. (wikibooks.org)

We introduce fragmentation at methylated loci and sequencing ( FML -seq), a sequencing library protocol that greatly reduces all these costs . (bvsalud.org)

Addressing these challenges requires a steep investment both in sequencing and bioinformatics infrastructure and capabilities. (qiagen.com)

The Mild-Seq course of might be repeated with completely different barcodes for various cell populations inside the identical pattern, which is left intact for follow-up evaluation. (nthenews.com)
Still, single-cell sequencing of RNA or epigenetic modifications can reveal cell-to-cell variability that may help populations rapidly adapt to survive in changing environments. (wikipedia.org)
PELE-Seq was evaluated using control E. coli populations and was then used to compare a wild C. remanei population to a lab-adapted population. (umn.edu)

The Seq trait serves as the basis for various concrete implementations, each with its own performance characteristics and use cases. (sparkcodehub.com)
By understanding the differences between these implementations and their respective performance characteristics, you can choose the most appropriate Seq type for your specific needs and write efficient, expressive, and maintainable Scala code. (sparkcodehub.com)

We found that identifications of at least two out of seven engines resulted in maximizing the protein identification power while minimizing the ratio of suspicious/translation-supported identifications (STR), as monitored by our STR index, based on RNC-Seq. (nih.gov)
TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. (thermofisher.com)
A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences. (thermofisher.com)

Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. (seqanswers.com)
To date, very large scale sequencing of many clinically important RNA viruses has been complicated by their high population molecular variation, which creates challenges for polymerase chain reaction and sequencing primer design. (ucl.ac.uk)
Major breakthroughs in medical genomics have been enabled by the implementation of clinical exome sequencing (ES) for molecular diagnosis, by genomics approaches for understanding the genetic basis of complex traits and diseases, and by progress in understanding genotype–phenotype relationships. (cdc.gov)

Live-seq enables temporal transcriptomic recording of single cells. (epfl.ch)

No discrepancies were observed across 118,757 nt compared between Sanger and our custom RNA-Seq method in 16 samples. (ucl.ac.uk)

The breakthrough method is called Live-seq and was jointly developed by scientists at EPFL and ETH Zurich. (epfl.ch)
Strong correlations were obtained between observed and expected read counts in all cases, with no evidence of length bias, showing that each method of RNA-seq is low bias. (nanoporetech.com)
Prefix sub , like in subseq(), means that the method applies to sub region of the sequence range and takes start and end as arguments. (metacpan.org)
Prefix named_ in method names allows the used to attach multiple meta strings to one sequence by explicitly naming them. (metacpan.org)
Single-cell sequencing was selected as the method of the year 2013 by Nature Publishing Group. (wikipedia.org)
Results: We have developed a wet-lab protocol and variant-calling method that identifies both sequencing and PCR errors, called Paired-End Low Error Sequencing (PELE-Seq). (umn.edu)
Conclusion: We have developed a method of rare allele detection that mitigates both sequencing and PCR errors, called PELE-Seq. (umn.edu)
The PELE-Seq method is ideal for investigating the dynamics of rare alleles in a broad range of reduced-representation sequencing methods, including targeted amplicon sequencing, RAD-Seq, ddRAD, and GBS. (umn.edu)
To bring F-seq up to speed with other technologies, we implemented a method to determine the distance between positive and negative strand sequence densities using the Wilcoxon-Mann-Whitney Rank Sum Test. (unc.edu)

Nevertheless, these "spatial transcriptomics" approaches nonetheless solely seize a fraction of a cell's complete RNA molecules, and can't ship the depth and high quality of research offered by single-cell sequencing strategies, which had been developed to analyze the transcriptomes of particular person cells remoted from tissues or biofluids by way of next-generation sequencing (NGS) methods. (nthenews.com)
With a efficiency corresponding to single-cell sequencing strategies, it considerably broadens the depth and scope of investigations doable on a tissue pattern. (nthenews.com)
Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. (wikipedia.org)
By deep sequencing of DNA and RNA from a single cell, cellular functions can be investigated extensively. (wikipedia.org)
It is more challenging to perform single-cell sequencing than sequencing from cells in bulk. (wikipedia.org)
Recent technical improvements make single-cell sequencing a promising tool for approaching a set of seemingly inaccessible problems. (wikipedia.org)
For example, heterogeneous samples, rare cell types, cell lineage relationships, mosaicism of somatic tissues, analyses of microbes that cannot be cultured, and disease evolution can all be elucidated through single-cell sequencing. (wikipedia.org)
Single-cell DNA sequencing has been widely applied in mammalian systems to study normal physiology and disease. (wikipedia.org)
Microfluidics entrepreneur Mark Gilligan provides a perspective on the development of single-cell sequencing technologies. (rsc.org)
Droplet-based single-cell sequencing pioneers Allon Klein and Evan Macosko summarise the growth of the technology and look ahead to future developments. (rsc.org)
Thought leader David Weitz introduces the Lab on a Chip droplet-based single-cell sequencing thematic collection. (rsc.org)
Established in 2019, CTG offers a dynamic, state-of-the-art platform for single-cell sequencing to users. (lu.se)

To further understand electrotactic migration of tumor cells, we performed RNA-sequencing for pathway discovery to identify signaling that is differentially affected by the exposure of direct-current electrical fields. (nature.com)
Differences in patient and tumor characteristics of BRCA1/2 carriers who were already identified as part of clinical BRCA1/2 testing routines and additional BRCA1/2 carriers found by sequencing the entire study population were compared using logistic regression models. (lu.se)

Our dedicated DNA sequencing kits for targeted digital DNA sequencing enable you to confidently detect low-frequency variants. (qiagen.com)
Whole-exome sequencing showed that a combination of common and rare, or even private variants, is responsible for COVID-19 susceptibility and severity," said Renieri. (genomeweb.com)

This takes as input sequence.mapview, only uses sequences aligning in fewer than 2 locations with 0 or 1 mismatches, and only sequences with an alignment score of 0 or better. (unc.edu)
Usage of reporter genes need to be specified in iLab before project start and the exact sequence information and gene co-ordinates need to be submitted to be included during sequencing read alignment. (lu.se)

Templates are sequenced using reversible, fluorescent-tagged terminator nucleotides. (utmb.edu)
In case they differ, the position of the mutated nucleotides has to be defined from the selected sequence. (lu.se)

I have 18 samples in total that were sequenced in 2 batches and I'm using Scater and scDblFinder for my QC. (bioconductor.org)

In Direct RNA sequencing, adapters are ligated onto the 3' end of poly-A-tailed RNA strands before sequencing (Fig. 1d). (nanoporetech.com)
With the assistance of a brand new DNA nanotechnology, the barcoded RNA sequences are then translated into coherent DNA strands, which may then be collected from the tissue pattern and recognized utilizing NGS. (nthenews.com)

What can you do with Next Generation Sequencing? (utmb.edu)

Meta provides basic, pure perl implementation of sequences with meta information. (metacpan.org)
Choose the appropriate Seq implementation for your specific use case based on performance characteristics and desired functionality. (sparkcodehub.com)
When choosing a Seq implementation, consider these performance characteristics and the requirements of your specific use case. (sparkcodehub.com)
A sequence number in JPA is a sequential id generated by the JPA implementation and automatically assigned to new objects. (wikibooks.org)

This test allows for a non-parametric estimate of the difference in positive-negative sequence distances from the expected value at 0 by ranking all possible positive-negative sequence differences and taking the median difference as the estimate. (unc.edu)

The novel methods for identifying accurate splice junctions and transcription start/end sites are widely applicable and will improve single-molecule sequencing analysis from any species. (dundee.ac.uk)
They also performed standard culturing methods to compare that to sequencing. (genomeweb.com)
Methods inherited from interface org.biojava.bio.seq. (biojava.org)

The enriched mtDNA is then subjected to library prep and then sequencing. (northwestern.edu)
Library construction cost for mtDNA-seq is listed on the Core Pricing page. (northwestern.edu)

This, coupled with the very limited DNA damage repair capability in the organelle, leads to sequence alterations in mtDNA at a much higher frequency than that in the nuclear DNA. (northwestern.edu)

Seq-DI™ Formamide is a proven, widely adopted and high quality sample loading reagent for 310, 3100, 3130 and 3730 Genetic Analyzers. (nimagen.com)

Our protocol increases the efficiency and decreases the time to complete a ddRAD-Seq experiment. (sagescience.com)

Single-molecule long-read sequencing technologies provide improved integrity of transcript structures including alternative splicing, and transcription start and polyadenylation sites. (dundee.ac.uk)

Anatomy6

Organisms6

Diseases4

Chemicals and Drugs49

Analytical, Diagnostic and Therapeutic Techniques and Equipment56

Phenomena and Processes124

Disciplines and Occupations11

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture3

Information Science13

Named Groups1

Health Care13

Geographicals1

ChIP Seq6

Transcriptome6

Genome9

ScRNA-seq5

Nucleotide9

High-throughput4

Quantification2

Libraries10

Reagents3

MRNA4

Genomic5

CDNA6

Restriction-site-associated1

Gene expression5

Assay2

Illumina6

Bart Deplancke1

RNAs3

Amplification2

Mutations3

Reads2

SNPs2

Annotation1

Nanopore1

Amino acid seq1

Researchers7

Primers1

Quantitative1

Data11

Loci1

Bioinformatics1

Populations3

Implementations2

Protein3

Molecular3

Enables1

Sanger1

Method9

Single-cell12

Tumor2

Variants2

Alignment2

Nucleotides2

Batches1

Strands2

Next-generation1

Implementation4

Differences1

Methods3

Library2

Alterations1

Widely1

Efficiency1

Technologies1