Sequences cloning molecular. Medical search. Frequent questions

In this article, we report cloning of the rat apelin receptor cDNA. (nih.gov)
To aid in the examination of this phenomenon, the cDNA sequences encoding estrogen receptor-alpha (esr1), estrogen receptor-beta-a (esr2a) and ovarian aromatase (cyp19a1a) for the yellow perch were obtained. (usda.gov)
The sequence of the alpha 2 beta cDNA shows the presence of a single Arg residue between Gln 142 of the alpha 2 chain and Ileu 1 of the beta chain. (rti.org)
3] "Cloning and nucleotide sequencing of transthyretin (prealbumin) cDNA from rat choroid plexus and liver. (tcdb.org)

The Ministry subsequently reported that this GBS belonged to ST283 ( 3 ), and its whole genome has been recently sequenced ( 4 ). (cdc.gov)
Whole-genome sequence contigs clone ST-3, which also originated in Asia ( 8 , 12 ). (cdc.gov)
Whole genome sequencing (WGS) is increasingly used for pathogen identification and surveillance. (eurosurveillance.org)
They were able to sequence the whole genome and confirm that the bacteria did not carry genetic markers for antibiotic resistance or evidence of genetic engineering in only 8.5 hours. (cdc.gov)
Experimental evolution coupled with whole genome sequencing can provide insights of the adaptive process. (datadryad.org)

Scholars@Duke publication: Molecular cloning and analysis of functional envelope genes from human immunodeficiency virus type 1 sequence subtypes A through G. The WHO and NIAID Networks for HIV Isolation and Characterization. (duke.edu)
Molecular and functional characterization of a SCD 1b from European sea bass (Dicentrarchus labrax L. (researchgate.net)

Subsequent cloning and sequencing of gcsap fragments obtained from PCR with genomic DNA revealed a 73 bp intron beginning at nt 728. (microbiologyresearch.org)
Established in the mid 1970's, New England Biolabs, Inc. (NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. (prnewswire.com)
It is well equipped with molecular and cellular biology technologies to conduct virus isolation, genomic sequencing, and cloning, through which determines the common virus strains circulating in the country. (who.int)
BACKGROUND: Genomic surveillance of SARS-CoV-2 is crucial for monitoring the spread of COVID-19 and guiding public health decisions, but the capacity for SARS-CoV-2 testing and sequencing in Africa is low. (bvsalud.org)
and (i i) to develop (epi)genomic powerful molecular and/or cell biology and close col aboration to create synergies methodologies, profiling strategies, and functional genomics tools, recent progress and better exploit and further expand bioinformatics tools and resources that in understanding of the cancer (epi) unique research tools and expertise. (who.int)

Comparison of the deduced amino acid sequences of the giant panda with those of these other species revealed that the RPS14 of giant panda is highly homologous with those of B. taurus, R. norvegicus and D. rerio (85.99, 99.34 and 99.34%, respectively), and is 100% identical with the others. (geneticsmr.com)
Analysis of their deduced amino acid sequences revealed several important differences from prototypic subtype B strains, including changes in the number and distribution of cysteine residues, substantial length differences in hypervariable regions, and premature truncations in the gp41 domain. (duke.edu)
One of these, derived from a patient with rapid CD4 cell decline, contained an amino acid substitution in a highly conserved endocytosis signal (Y721C), as mediated virus entry with very poor efficiency, although they did not contain sequence changes predicted to alter protein function. (duke.edu)
a ) Amino acid sequence comparison of human and mouse podocin. (jci.org)
With a few minor exceptions, the DNA sequence fits the previously published amino acid sequences. (rti.org)
Based on the deduced sequence, the mature enzyme contains 329 amino acids and shows approximately 40% identity to other fungal aspartic proteinases. (microbiologyresearch.org)
Amino acid sequence of rhizopuspepsin isozyme pl5. (microbiologyresearch.org)
The nucleotide and deduced amino acid sequences of preprochensinin-1 were not similar to any known amphibian skin defensive peptides. (bioone.org)
Four bioactive peptides were chemically synthesized according to the deduced amino acid sequences of six prepropeptides from R. chensinensis skin, and their antimicrobial, cytotoxic, and haemolytic properties were evaluated. (bioone.org)
The Open reading frame (ORF) of the AnpeNPV consists of 738 nucleotides encoding 245 amino acids with molecular masses of 29 kDa. (scirp.org)
Prediction of protein antigenic determinants from amino acid sequences. (scirp.org)

Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications. (sigmaaldrich.com)

Worldwide, the predominant clone belongs to serotype III, subtype 2 (III-2), sequence type (ST) 17 ( 1 ), which is less genetically diverse and heterogeneous than strains from the other serotypes (Ia, Ib, II-IX). (cdc.gov)
Monitoring the long-term molecular epidemiology of the pneumococcus and detection of potential 'vaccine escape' strains. (jcvi.org)
Therefore, multi-locus sequence typing (MLST)-defined clones could conceivably include strains from multiple PspA clades. (jcvi.org)
In addition, malaria strains, genetic polymorphism and the molecular phylogeny of Yemen Plasmodium isolates will be studied. (biomedcentral.com)
Molecular diagnostics now enables detecting viruses in clinical and environmental specimens, linking of NLV strains causing outbreaks in multiple geographic locations, and tracing them to their sources in contaminated food or water. (cdc.gov)

Using a combination of next-generation sequencing, bioinformatics, and molecular cloning, CDC can identify the functions of the individual antibodies and what part of the influenza virus they target. (cdc.gov)

E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
Molecular cloning, nucleotide sequence, and gene expression in liver and brain. (tcdb.org)

Thus, the molecular approach was applied for the first time, in this country, to investigate the molecular epidemiology of malaria. (biomedcentral.com)
Our objective was to characterize the molecular epidemiology and AMR mechanisms of this pathogen. (who.int)

PCR (polymerase chain reaction) technique has achieved increased importance for post-mortem DNA analysis in forensic cases 8 because of the millions of copies amplified from one specific sequence of DNA. (bvsalud.org)

Scholars@Duke publication: Molecular cloning of the gene sequences of a major apoprotein in avian very low density lipoproteins. (duke.edu)

We have sequenced the cloned DNA and have found no homology between the Na + /glucose co-transporter and either the mammalian facilitated glucose carrier or the bacterial sugar transport proteins. (nature.com)
In contrast, the major satellite DNA sequences that had a low sequence homology are present only in a few species. (edu.au)

These sequences are valuable molecular tools that can be used in future studies investigating estrogen mechanisms and actions, such as SSD, in yellow perch. (usda.gov)
The work package focusing on molecular studies generated new evidence about the changes effected by antibiotic therapy on commensal organisms or opportunistic pathogens in the oropharyngeal, nasal and gastro-intestinal flora and study AMR mechanisms and the dissemination of successful clones of fluoroquinolone-resistant, carbapenem-resistant or extended-spectrum beta-lactamase harboring Gram-negative bacteria, MRSA and fluoroquinolone-resistant viridans streptococci. (europa.eu)
My research focuses on the molecular mechanisms and biomarkers of rejection after allograft transplantation. (stanford.edu)
Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)

The result of all these early efforts can be seen in the fact that molecular diagnostic tests are now on the market for most, if not all, of these genetic diseases. (genengnews.com)
Fatty acid desaturation is a highly complex and regulated process involving different molecular and genetic actors. (researchgate.net)
Modern genetic techniques have helped us hugely in characterizing these disorders and providing a molecular diagnosis in the face of nonspecific clinical data. (medscape.com)
Gene technology: basic and applied molecular genetic methods. (lu.se)
The lab consists of molecular tools that can be used for genetic modification of neurons. (lu.se)

tern of dominance afterwards because of the presence of In silico multilocus sequence typing (MLST) by multiple serotypes. (cdc.gov)

METHODS: In this molecular epidemiological analysis, which is part of a wider multifaceted prospective observational study, we collected national SARS-CoV-2 test data from 35 sites across 16 regions in Ghana from Sept 1, 2020, to Nov 30, 2021, via the Ghanaian integrated influenza and SARS-CoV-2 surveillance network. (bvsalud.org)

Experience with Illumina sequencing platforms and library preparation methods. (biospace.com)

Subsequently a stable CHO cell line expressing the receptor fused at its C-terminal part with the enhanced green fluorescent protein (EGFP) was established, allowing to verify its cell surface distribution and to determine the affinity of various apelin and angiotensin fragments on the cloned receptor. (nih.gov)
The novel, proprietary DNA polymerase in the NEBuilder HiFi enzyme mix results in extremely accurate fragment assemblies and cloning junctions, and can assemble DNA fragments ranging from 100 bp to at least 19 kb. (prnewswire.com)
Scientists using fragments designed using other seamless cloning methods can easily assemble these with NEBuilder HiFi, due to compatibility of the fragments. (prnewswire.com)

In an effort to gain more understanding into the yellow perch E2 stimulated SSD, the cDNAs for genes that encode for hormones that are involved with the endocrine growth axis (GH, PRL, SL and IGF-I) have been recently cloned and characterized. (usda.gov)
In this current paper, we have cloned and sequenced the full length cDNAs for ER? (usda.gov)
In the present study, six cDNAs encoding amphibian skin antimicrobial peptide precursors from the Chinese brown frog Rana chensinensis , were cloned and identified as preprobrevinin-1CEa, preprobrevinin-1CEb, preprotemporin-1CEa, preprotemporin-1CEb, preprotemporin-1CEc, and preprochensinin-1. (bioone.org)

Nuclear genome sequence and gene expression of an intracellular fungal endophyte stimulating the growth of cranberry plants. (umontreal.ca)
The genome sequence of the multinucleocapsid nucleo-polyhedrovirus of the Chinese oak silkworm Antheraea pernyi. (scirp.org)
This analysis, which marks 22 years since the publication of the first Typhi genome, represents the largest Typhi genome sequence collection to date (n=13,000). (cdc.gov)

In: Expressed sequence tags - generation and analysis, Methods in Molecular Biology Vol. 533 (J. Parkinson, ed. (umontreal.ca)
My current research focus is biomarker discovery of graft rejection in solid organ transplant patients and studying natural killer cells in viral infection using omics methods including Mass Cytometry and Next Generation Sequencing. (stanford.edu)
We report the methods used for marker development, along with the primer sequences and optimized PCR and thermal cycling conditions. (mdpi.com)
From the 86 positive blood samples, 13 Plasmodium falciparum and 4 Plasmodium vivax were selected and underwent cloning and, subsequently, sequencing and the sequences were subjected to phylogenetic analysis using the neighbor-joining and maximum parsimony methods. (biomedcentral.com)
METHODS: This is a meta-analysis of global genotype and antimicrobial resistance (AMR) determinants extracted from previously sequenced genome data and analysed using consistent methods implemented in open analysis platforms GenoTyphi and Pathogenwatch. (cdc.gov)
Methods for gene identification and analysis of gene structure: cloning, PCR, restriction mapping, in situ hybridisation, DNA sequencing. (lu.se)

Though microbial ecology of the gut is now a major focus of interest, little is known about the molecular determinants of microbial adaptation in the gut. (datadryad.org)

We have purified and cloned a HeLa cell nuclear protein that strongly stimulates binding of retinoic acid and thyroid hormone receptors (RARs and TRs) to response elements. (nih.gov)
When it was fused with the N-terminally His-tagged protein, it gave rise to accumulation of an expected 22-kDa polypeptide, in good agreement with the predicted molecular weight. (geneticsmr.com)
report the molecular cloning of Painting of fourth (Pof), which encodes a protein that specifically paints chromosome 4 (Proc Natl Acad Sci USA 2001, 98:6273-6278). (the-scientist.com)
The POF protein sequence contains a putative RNA-binding domain. (the-scientist.com)
The gcsap open reading frame encodes a protein with a 78 residue prepro-sequence typical of other fungal secreted aspartic proteinases. (microbiologyresearch.org)
First waikavirus infectious clones and vascular expression of green fluorescent protein from maize chlorotic dwarf virus (MCDV). (usda.gov)
To demonstrate the utility of these markers, we provide haplotype networks, DNA alignments, and summary statistics regarding the sequence variation for the two protein-coding nuclear loci (FEM1 and UbiA). (mdpi.com)
Here, we report that the recently sequenced genome from the red flour beetle Tribolium castaneum contains a gene coding for an oxytocin/vasopressin-like peptide, identical to the locust peptide, which we named inotocin (for insect oxytocin/vasopressin-like peptide) and a gene coding for an inotocin G protein-coupled. (lu.se)
Bioinformatic analyses of DNA- and protein sequences. (lu.se)

We report the molecular cloning of DNA complementary to human Hp mRNA. (rti.org)

Phylogenetic analyses of full-length env sequences indicated that the panel included members of all major sequence subtypes of HIV-1 group M (clades A to G), as well as an intersubtype recombinant (F/B) from an infected individual in Brazil. (duke.edu)
In summary, the overarching rationale of SATURN was to improve methodological standards and conduct research that will help to better understand the impact of antibiotic use on acquisition, selection and transmission of AMR in different environments, by combining analyses of molecular, individual patient-level and ecologic data. (europa.eu)
Also, SATURN had as mission to improve methodological standards and conduct research to better understand the impact of antibiotic use on acquisition, selection and transmission of antibiotic-resistant bacteria (ARB) in different environments, by combining state-of-the-art analyses of molecular, ecologic and individual patient-level data. (europa.eu)
Currently, with the application of molecular biology resources in human identification, it became possible to identify a person even without physical ante-mortem data or with deteriorated biological material in negligible amounts, which are actually relatively common conditions in forensic analyses 7,12,16,19 . (bvsalud.org)

During further processing, the 110-kd polypeptide is reduced to a molecular weight of 84 kd. (medscape.com)

To generate a more representative panel of genetically diverse envelope genes, we PCR amplified, cloned, and sequenced complete gp160 coding regions of 35 primary (peripheral blood mononuclear cell-propagated) HIV-1 isolates collected at major epicenters of the current AIDS pandemic. (duke.edu)
The Core serves as a centralized resource for cloning and sequencing of genes. (unc.edu)
In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)

The minor repetitive sequences are present in the genomes of the other seven Apodemus species. (edu.au)
Differentiation of Schistosoma haematobium from related species using cloned ribosomal RNA gene probes. (ajtmh.org)
Although malaria in Yemen is caused mainly by Plasmodium falciparum and Plasmodium vivax , there are no sequence data available on the two species. (biomedcentral.com)
This study was conducted to investigate the distribution of the Plasmodium species based on the molecular detection and to study the molecular phylogeny of these parasites. (biomedcentral.com)
Much work on molecular phylogeny has focused on the relationship between Plasmodium species and the origin of human malaria [ 5 - 7 ]. (biomedcentral.com)
The inotocin/receptor couple can also be found in the recently sequenced genome from the parasitic wasp Nasonia vitripennis but not in any other holometabolous insect with a completely sequenced genome (12 Drosophila species, the malaria mosquito Anopheles gambiae, the yellow fever mosquito Aedes aegypti, the silk worm Bombyx mori, and the honey bee Apis mellifera), suggesting that this neuropeptide system is confined to basal holometabolous insects. (lu.se)

The sequence lineages were typed with pangolin and the phylogenetic analysis was conducted with IQ-Tree2 and TreeTime. (bvsalud.org)

This analysis identified 20 clones, including 1 from each subtype (or recombinant), which expressed fully functional envelope glycoproteins. (duke.edu)
Molecular cloning and Western blot analysis of mouse podocin. (jci.org)
W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. (scirp.org)
S. Gomi, K. Majima, Maeda S. (1999) Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus. (scirp.org)
Sequence alignment analysis showed 5-14.8% SNP in the partial sequences of the 18 s rRNA of P. falciparum . (biomedcentral.com)
NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis. (prnewswire.com)
Overall, we show that "evolve and sequence" approach coupled to an analysis of convergence, when applied to a natural isolate, can be used to study adaptation in vivo and uncover the specific selective pressures of that environment. (datadryad.org)
After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)

We used PCR, pulsed-field gel electrophoresis, and multilocus sequence typing for serotyping and subtyping of the isolates, as described ( 1 , 6 ). (cdc.gov)

As a Senior Molecular Biologist, you will be responsible for developing and implementing molecular biology techniques to support our research programs. (jobs.ac.uk)
Training in molecular biology techniques. (unc.edu)

P. aeruginosa isolates referred to the ARSP, epidemic clone. (who.int)

Developing a framework to assess the costeffectiveness of COMPARE - a global platform for the exchange of sequence-based pathogen data. (eurosurveillance.org)

In: RNA Metabolism in Mitochondria, book series "Nucleic Acids and Molecular Biology" (eds J.Cruz-Reyes, MW. (umontreal.ca)

Contribute to the development of novel antibody sequencing approaches and workflows for both bulk and single cells. (biospace.com)
Sequencing these antibodies provides a high-resolution view of how the human immune system responds to an influenza vaccine, how each specific antibody functions, and what part of the influenza virus they target. (cdc.gov)

The Molecular Biology Core was established to provide expertise, equipment, training, and reagents to serve the molecular biology needs for the Marsico Lung Institute/UNC Cystic Fibrosis Center. (unc.edu)
NEB has supplied DNA assembly reagents for over 40 years and the new NEBuilder HiFi DNA Assembly Master Mix is the result of our commitment to producing high quality, innovative cloning reagents. (prnewswire.com)

The SATURN project had as aim to study the impact of antibiotic exposure on AMR with a multidisciplinary approach that bridges molecular, epidemiological, clinical and pharmacological research. (europa.eu)

They have complementary skills in the fields of molecular and cellular virology, immunology, and physiopathology of infectious diseases. (europa.eu)

In addition, all subtype E and three subtype G viruses initially classified on the basis of partial env sequences were found to cluster in subtype A in the 3' half of their gp41 coding region, suggesting that they are also recombinant. (duke.edu)
As a biochemist you study the way living organisms - from viruses and bacteria to mammals, plants and other higher organisms - function at the molecular level. (kent.ac.uk)
Occurrence and high throughput sequencing of viruses in Ohio wheat. (usda.gov)
CDC has developed a process that allows scientists to sequence the antibodies from the human B cells that make up part of the immune response to influenza viruses. (cdc.gov)
Norwalk-like viruses:" public health con- sequences and outbreak management. (cdc.gov)

The Homingbac bacu-lovirus cloning system: An alternative way to introduce foreign DNA into baculovirus genomes. (scirp.org)

In your first year, your modules give you an insight into various biological and chemical disciplines, including biochemistry, cell and molecular biology, microbiology and physiology. (kent.ac.uk)

The type II minor repetitive sequence also hybridized interspersedly in the non-centromeric regions. (edu.au)
repetitive sequence elements in the members had been in contact with bacterial genome have shown con- patients for several hours. (who.int)

We describe a hypervirulent clone of group B Streptococcus serotype III, subtype 4, sequence type 283, that caused invasive disease with a predilection for meningitis in Hong Kong during 1993-2012. (cdc.gov)

The sequence data of mouse podocin are available from GenBank/EMBL/DDBJ under accession no. (jci.org)
Molecular data have been an integral tool in the resolution of the evolutionary relationships and systematics of freshwater mussels, despite the limited number of nuclear markers available for Sanger sequencing. (mdpi.com)
Data gaps remain in many parts of the world, and we show the potential of travel-associated sequences to provide informal 'sentinel' surveillance for such locations. (cdc.gov)

IPSWICH, Mass. , Jan. 30, 2015 /PRNewswire/ -- New England Biolabs (NEB®) announces the introduction of the NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix for one-step cloning and multiple DNA fragment assembly in as little as 15 minutes. (prnewswire.com)
The same reagent allows fast, virtually error-free standard cloning, as well as large, complex multi-fragment DNA assemblies. (prnewswire.com)

We found evidence of persistence or reintroduction of the predominant clone ST235 in one hospital, and of transfer between hospitals. (who.int)

Witnosky goes on to point out that the two most common applications for molecular tests are: identifying and profiling infectious disease agents and monitoring patient therapeutic regimens. (genengnews.com)

This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. (sigmaaldrich.com)
The locus at chromosome 1q21 was identified by linkage mapping in 1998, but the gene has only recently been discovered due to difficulty with sequencing this highly repetitive region and was previously missed using next-generation sequencing. (medscape.com)

SARS-CoV-2-positive samples collected through this integrated national influenza surveillance network and from international travellers arriving in Accra were sequenced with Oxford Nanopore Technology sequencing and the ARTIC tiled amplicon method. (bvsalud.org)

In this work, we used ochratoxin A (OTA) as a model system to study the capacity of PD-IPCR in the detection of toxic small molecular weight compounds, especially mycotoxins. (cdc.gov)
This study provides a new idea for the ultrasensitive detection of mycotoxins and other toxic small molecular weight compounds. (cdc.gov)

For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported. (sigmaaldrich.com)

Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors, including molecular diagnostics development. (prnewswire.com)

Having trained in the disciplines of immunology, molecular biology, and biotechnology, I have always been interested in applying state-of-the-art technologies to study human diseases and conditions. (stanford.edu)

Initially illnesses were reported only in the genome sequencing of clinical and environmental samples from the outbreak revealed a new V. parahaemolyticus northern cities of Peru (Cajamarca, Chiclayo, and Piura), clone. (cdc.gov)

The technical platform includes a general molecular laboratory and a class2 cellular laboratory at A11 BMC Lund. (lu.se)

This review details the anatomy, development, physiology, and molecular characteristics of the Lemnaceae to introduce them to the broader plant research community. (osti.gov)

It's the most powerful molecular motor known. (lu.se)

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