Sequences rna untranslated. Medical search. Frequent questions

This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution. (nih.gov)
These proteins were identified experimentally through co-expression or affinity capture-RNA. (wikipedia.org)
The method enables a comprehensive evaluation of significantly mutated areas in both coding and noncoding regions of DNA that affect the sites for RNA-binding proteins. (aacr.org)
In the second conformation, the RNA remains in the host cell to be translated into new viral proteins. (europa.eu)
RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
Using ribosome profiling, I wanted to see how the binding of ribosomes to RNA related to the timing of when those proteins actually got made. (eurekalert.org)
Or you may see its SNR and P. And this is the combination of the small nuclear RNA and the proteins that make up the splices own. (pearson.com)
NEW YORK (GenomeWeb) - A Massachusetts Institute of Technology-led research team this month reported new data suggesting that an siRNA targeting a messenger RNA's 3' untranslated region (UTR), rather than its coding sequence (CDS), can harness the activity of multiple Argonaute proteins to better suppress its target. (genomeweb.com)
They have also all have been shown to bind microRNAs and siRNA indiscriminately of sequence to interact with a common set of helicases and mRNA-binding proteins, and to localize to P-bodies in mammalian cells with a capability of targeting mRNAs to the general eukaryotic machinery for translation control and mRNA degradation. (genomeweb.com)
MicroRNAs (miRNAs) are short (21-24 nucleotides) sequences of RNA that reduce gene expression by promoting the breakdown of messenger RNAs (mRNAs) and by repressing translation of mRNAs into proteins. (freethoughtblogs.com)
Researchers have now created a new way to deliver various types of RNA cargo to cells, which utilizes one of the human body's natural proteins to create particles that can bind to RNA. (labroots.com)
Based on sequence similarity, RNA-binding specificity, and functional regulation of translation, the CPEB proteins have been classified into two subfamilies. (nih.gov)
Professor Jin and her lab propose that structured 5'UTRs can facilitate translation initiation via sequential remodeling: the 5'UTR undergoes sequential conformational changes that are facilitated by RNA binding proteins and translational factors, which ultimately leads to recruitment of the ribosome for translation of the mRNA. (illinois.edu)
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. (wikidata.org)
Quantitative comparisons of RNA-binding sites showed that NXF1 and SR proteins bind mRNA targets at adjacent sites, indicative of cobinding. (uni-frankfurt.de)

The BN rat sequence is the third complete mammalian genome to be deciphered, and three-way comparisons with the human and mouse genomes resolve details of mammalian evolution. (nih.gov)
The sequence of the complete genomes including the HA and NA UTRs of low and high yield reassortant vaccine candidate, and seed stocks and their parental viruses (wild type). (jcvi.org)
During the past decade, two major developments-the emergence of massively parallel, ultra-cheap DNA sequencing technologies and the use of these technologies as digital readouts for functional genomic assays-have led to a profusion of data describing various features of genomes, epigenomes, and transcriptomes 1 , 2 . (biorxiv.org)
This analysis revealed previously unidentified alterations in untranslated regions, long noncoding RNAs, and introns that may guide the interpretation of noncoding variants in cancer genomes. (aacr.org)
To gain in-depth insights into the viral population and to perform detailed characterization of viral genomes, we used a new approach combining long-range PCR with next-generation and third-generation sequencing which allowed near full-length sequencing of HEV-3 genomes. (nature.com)
Furthermore, we developed a targeted ultra-deep sequencing approach to assess the dynamics of clinically relevant mutations in the RdRp-region and to detect insertions in the HVR-domain in the HEV genomes. (nature.com)
The HCV RNA polymerase is highly error prone, and the sequences of HCV genomes display enormous amounts of variation. (biomedcentral.com)
Each RBH couple consists of two genes, one from each of the two genome sequences (or genomes) to compare, which display the highest scoring hit in the other data set in a reciprocal manner [ 36 ]. (biomedcentral.com)
Many of these viruses have RNA genomes. (bvsalud.org)

To address the mechanism of isoform-specific mRNA localization, molecular chimeras were constructed by insertion of actin sequences between the Lac Z coding region and SV-40 3'UTR in a reporter plasmid. (umassmed.edu)
In contrast, sequences from the 5'UTR through the coding region of either actin gene did not localize the blue product. (umassmed.edu)
Recently a relatively well-conserved sequence of the non-coding region has been identified, permitting the development of an inexpensive and highly accurate diagnostic polymerase chain reaction (PCR)-based blood test for the virus and its titer [ 6 ]. (biomedcentral.com)
The nOPV2 candidate strains include different combinations of 5 distinct modified regions of the Sabin-2 genome, including changes to the RNA sequence in the 5' untranslated region of polio genome (5' UTR), the capsid protein coding region (P1), the non-structural protein 2C, and the polymerase 3D. (biosafety.be)

Therefore, 3'UTR sequences play a key role in modulating the distribution of actin mRNAs in muscle cells. (umassmed.edu)
A four-nucleotide translation enhancer in the 3′-terminal consensus sequence of the nonpolyadenylated mRNAs of rotavirus. (microbiologyresearch.org)
The role of the 3′-untranslated region of non-polyadenylated plant viral mRNAs in regulating translational efficiency. (microbiologyresearch.org)
The mRNAs can carry short sequences of genetic letters that can trigger their own destruction. (elifesciences.org)
The researchers found many upstream open reading frames (uORFs) in the 5' untranslated region of circadian mRNAs, which is the part of RNA before the so-called "coding sequence" that gets translated by ribosomes into protein. (eurekalert.org)
Analyzing bacteria in late exponential phase, we capture ~40% ( E. faecalis ) and 43% ( E. faecium ) of the annotated protein-coding genes, determine 5′ and 3′ UTR (untranslated region) length, and detect instances of leaderless mRNAs. (frontiersin.org)
CPEB-1 is an RNA-binding protein that interacts with the cytoplasmic polyadenylation element (CPE), a short U-rich motif in the 3' untranslated regions (UTRs) of certain mRNAs. (nih.gov)
It preferentially binds to poly(U) RNA oligomers and may regulate the translation of stored mRNAs during spermiogenesis. (nih.gov)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA. (hindawi.com)
miRNAs act as adaptors that employ a silencing complex to target mRNAs by selective base pairing, primarily in the 3′-untranslated region (3′-UTR). (hindawi.com)
MiRNAs, short (18-24 nucleotides) non-coding RNAs, are derived from long transcripts pri-miRNAs and pre-miRNAs [ 26 - 30 ].By targeting 3' untranslated regions (3'UTRs) of cognate mRNAs, miRNAs post-transcriptionally regulate gene expression and induce translational repression [ 29 , 30 ]. (intechopen.com)
We have reported previously that Short Interspersed Degenerate Retroposons of the SIDER2 subfamily, largely located within 3'UTRs of Leishmania transcripts, promote rapid turnover of mRNAs through endonucleolytic cleavage within the highly conserved second tandem 79-nt hallmark sequence (79-nt SII). (uni-frankfurt.de)

Hepatitis C RNA: Testing for HCV RNA by reverse-transcriptase ¬polymerase-chain reaction (RT-PCR) amplification of the 5' noncoding region was performed on anti-HCV positive samples. (cdc.gov)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
Of these modifications, only the changes to polymerase 3D result in a change in the amino acid sequence. (biosafety.be)

As displayed in the multiple sequence alignment to the right, strict orthologs such as mammals and reptiles have highly conserved regions. (wikipedia.org)
Evolutionarily conserved untranslated regions facilitate the cloning of complete coding sequences of chondriogenes encoding NADH dehydrogenase subunits. (techscience.com)
It is a hepatotropic, approximately 7.2 kb single-stranded positive-sense RNA virus whose genome contains three open reading frames (ORF1, ORF2, and ORF3), 5′ and 3′ untranslated regions (UTRs), and a poly(A) tract at the 3′ end. (nature.com)
We find that the fission yeast homologues of Tristetraprolin/TTP and Pumilio/Puf (Zfs1 and Puf3) interact with Ccr4-Not via multiple regions within low-complexity sequences, suggestive of a multipartite interface that extends beyond previously defined interactions. (elifesciences.org)
Untranslated regions. (pearson.com)
Then they engineered PEG10's untranslated regions to add various RNA molecules that would also be identified and packaged by PEG10. (labroots.com)
In addition, use of pathogenicity prediction tools for noncoding variants identified with cWGS including deep intronic and untranslated regions (UTR) remains limited. (hhs.gov)
To increase diagnostic success of Washington University in St. Louis (WUSTL) UDN Phase 2 participants with non-diagnostic clinical and genomic evaluations, we propose performing RNA-Seq transcriptomic analyses, long-read DNA genomic sequencing, and functional studies of noncoding variants in deep intronic or untranslated (5 and 3' UTR) regions. (hhs.gov)
Furthermore, 5'-untranslated regions (5'UTRs) of mRNA are essential for translation. (illinois.edu)
Predictions of untranslated regions were also updated by harnessing the potential of Araport11's information, which was generated by using high coverage RNA-Seq data. (biomedcentral.com)
Based on the nucleotide sequence information of transcripts, we identified 819 novel transcriptionally active regions (nTARs) and revised 906 incorrectly predicted gene models, including revisions of exon-intron structure, gene orientation and sequencing errors. (biomedcentral.com)
The rest of the modifications aim to stabilize the genetic sequence against reversion in either the 5' UTR or capsid regions. (biosafety.be)
The student will be responsible for improving the annotations, especially for the untranslated regions that lie up and downstream of protein coding genes. (lu.se)
The student will evaluate gene expression in relation to measures of genetic diversity, differentiation, and neutrality across the genome to evaluate whether the sequences or the flanking regions of genes with stage-specific expression demonstrate signs of selection. (lu.se)
The parts of the messenger RNA sequence that do not code for product, i.e. the 5' UNTRANSLATED REGIONS and 3' UNTRANSLATED REGIONS. (bvsalud.org)

In the recent past, the presence and function of a ROSE-like RNA-thermometer located in the 5′UTR of the rhamnosyltransferase genes rhlAB has been reported in wild type P. aeruginosa . (springer.com)
A better understanding of how these species cause disease and spread antibiotic resistance requires a knowledge of how its genes are controlled, on both the DNA and the RNA level. (frontiersin.org)
The subsequent huge changes in morphology were more often due to changes in when and where those genes were expressed than to changes in the coding sequences of the genes themselves. (freethoughtblogs.com)
Mattick, J.S. (2023) A Kuhnian revolution in molecular biology: Most genes in complex organisms express regulatory RNAs. (blogspot.com)
These observations suggest that the original conception of genetic information was deficient and that most genes in complex organisms specify regulatory RNAs, some of which convey intergenerational information. (blogspot.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
Moreover, 5′- and 3′-UTR sequences of 4378 genes have been retrieved and alternatively spliced variants of 69 genes have been identified. (biomedcentral.com)
We successfully employed deep sequencing RNA-Seq data in combination with an elaborate bioinformatics strategy in order to identify novel genes, incorrect gene models and mechanisms of transcript processing in the corn anthracnose fungus C. graminicola . (biomedcentral.com)
Sequence data of the revised genome annotation including several hundreds of novel transcripts, improved gene models and candidate genes for alternative splicing have been made accessible in a comprehensive database. (biomedcentral.com)

Its longest isoform consists of 4 exons, a coding sequence of 1881 nucleotides, and an upstream in-frame stop codon. (wikipedia.org)
This isofrom has a mRNA sequence of 1343 nucleotides. (wikipedia.org)
Many computational methods for gaining functional insights from sequence data are based on the simple, but powerful, observation that functionally important nucleotides tend to remain unchanged over evolutionary time, because mutations at these sites generally reduce fitness and are therefore eliminated by natural selection 7 - 15 . (biorxiv.org)
MicroRNAs (miRNAs) is a large family of endogenous, small noncoding RNAs with 20-25 nucleotides that have emerged as key posttranscriptional regulators of gene expression in mammals, bacteria, and plants [ 1 - 4 ]. (hindawi.com)
The putative cleavage site(s) and other nucleotides predicted to alter the RNA secondary structure of 79-nt SII were either deleted or mutated and their effect on mRNA turnover was monitored using a gene reporter system. (uni-frankfurt.de)

This comprehensive global TSS mapping atlas provides a valuable resource for RNA biology and gene expression analysis in the Enterococci. (frontiersin.org)
MicroRNAs (miRNAs) serve a role in gene expression, primarily by interacting with messenger RNAs, and may be potential biomarkers for detecting cancer. (spandidos-publications.com)
Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
Noncoding RNA sequences can regulate gene expression via interactions with epigenetic and other control mechanisms. (stanford.edu)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
miRNAs are small noncoding RNAs that have broad regulatory functions on gene expression. (lu.se)
The student will analyze gene expression in a reference-based pipeline using existing RNA sequencing data from larvae, pupae, and adult flies from each of the two host forms. (lu.se)

For these samples we will only need to add RNA ligation RT-PCR data to complete the UTRs. (jcvi.org)

microRNAs (miRNAs) are important regulators of target messenger RNA translation by binding mainly to complementary sequences of the 3′ untranslated region of target messenger RNA transcripts thereby leading to RNA degradation and/or inhibition of protein synthesis [ 4 ]. (hindawi.com)

We thought uORFs would primarily affect mRNA translation, but it turns out they might also trigger an RNA degradation response similar to nonsense mediated decay. (eurekalert.org)
And that cap protects the RNA for degradation. (pearson.com)
This protein can bind to RNA and will typically form a capsule around RNA molecules, protecting them from degradation. (labroots.com)
Here, we used site-directed mutagenesis and in silico RNA structural studies to delineate the cis-acting requirements within 79-nt SII for cleavage and mRNA degradation. (uni-frankfurt.de)
Furthermore, single or double substitutions of the dinucleotides targeted for cleavage as well as mutations altering the predicted RNA secondary structure encompassing both cleavage sites also prevent mRNA degradation, confirming that these dinucleotides are the bona fide cleavage sites. (uni-frankfurt.de)

miRNA is a non-coded RNA with a length of 19 - 25 bases. (scirp.org)
Here, we provide a comprehensive global analysis of the evolutionarily distant unicellular green alga Chlamydomonas reinhardtii to quantify the effects of miRNA on protein synthesis and RNA abundance. (freethoughtblogs.com)
Both types of small RNAs use a Drosophila melanogaster pre-miRNA-1 based hairpin for their expression, either with perfect base-pairing in the stem region (shRNA-like) or containing two mismatches (miRNA-like). (bvsalud.org)
One of the methods that does seem to work well is TargetScan, which relies on both pairing of seed sequences (a subset of the 18-23 bp that make up a miRNA) and phylogenetic conservation of sites. (lu.se)

We report here the genome sequence of the Brown Norway (BN) rat strain. (nih.gov)
The Arabidopsis thaliana Niederzenz-1 genome sequence was recently published with an ab initio gene prediction. (biomedcentral.com)
Since non-canonical splice sites are difficult to predict ab initio, we checked for options to improve the annotation by transferring annotation information from the recently released Columbia-0 reference genome sequence annotation Araport11. (biomedcentral.com)
The improved gene set of the Nd-1 genome assembly (GeneSet_Nd-1_v1.1) was evaluated via comparison to the initial gene prediction (GeneSet_Nd-1_v1.0) as well as against Araport11 for the Col-0 reference genome sequence. (biomedcentral.com)
RNA-Seq data ("external hints") does not support the detection and annotation of non-canonical splice sites on genome sequence assemblies at a satisfying level [ 26 , 27 , 28 ]. (biomedcentral.com)
Comparative analysis of RNA-Seq data and the genome sequence did not provide evidence for RNA editing in C. graminicola . (biomedcentral.com)
The full-length viral genome sequence was characterized coronaviruses (9). (cdc.gov)

Manual inspection of RNA-Seq read mapping and RT-PCR were applied to validate the structural annotations of non-canonical splice sites. (biomedcentral.com)

A. RNA ligation is used to link the 5' and 3' termini, which circularizes the genomic RNAs, then we use RT-PCR to amplify the region across the junction that contains the UTR. (jcvi.org)
It can be accessed online at www.helmholtz-hiri.de/en/datasets/enterococcus through an instance of the genomic viewer JBrowse. (frontiersin.org)
An annotated genomic sequence of the corn anthracnose fungus Colletotrichum graminicola has been published previously, but correct identification of gene models by means of automated gene annotation remains a challenge. (biomedcentral.com)

PROJECT SUMMARY Clinical whole genome sequencing (cWGS) is increasingly being used as a diagnostic tool for critically ill infants and children. (hhs.gov)
Whole genome sequencing was attempted for specimens with cycle threshold values <30. (cdc.gov)
We have extensive whole genome and RNA sequencing data taken from whole samples of flies at three life stages: larva, pupa and adult. (lu.se)

SRSF3 emerged as the most potent NXF1 adaptor, conferring sequence specificity to RNA binding by NXF1 in last exons. (uni-frankfurt.de)

To improve our understanding of the biological and clinical significance of somatic mutations on RNA processing in cancer, the authors developed a new method, known as MIRA (mutation identification for RNA alterations). (aacr.org)
In conjunction with RNA sequencing data, the researchers used MIRA to identify mutations in binding sites that altered RNA expression and splicing. (aacr.org)

This project will explore determine how sequence variation in the UTR's of the influenza vRNA's (particularly, HA and NA) impact the replication/fitness of influenza A viruses by focusing our analysis on the UTR's of high yield reassortants used as vaccine seed stocks and selected naturally circulating strains. (jcvi.org)
These two conformations therefore act like a molecular switch to direct the fate of the viral RNA, and thus viral replication. (europa.eu)
Eilat virus, a unique alphavirus with host range restricted to insects by RNA replication. (microbiologyresearch.org)
The 3′-untranslated region (UTR) of the non-segmented, negative-strand (NNS) RNA viral genome is called the leader sequence, and functions as the promoter for viral replication and transcription. (elsevierpure.com)
Exogenously provided, e.g. transgene encoded, small RNAs could be used to inhibit virus replication, breaking the transmission cycle. (bvsalud.org)
Coronaviruses are enveloped, positive-stranded RNA analysis of the consequences of virus replication in Vero viruses associated with enteric and respiratory diseases in cells. (cdc.gov)

With maximum values of 0.23 g/(g h) at 37 °C, this constitutes a more than 60% increase compared to the production rate of 0.14 g/(g h) at the growth optimum of 30 °C. Interestingly however, control experiments unveiled that besides the regulatory effect of the RNA-thermometer, multiple metabolic effects may contribute equally to the observed increase in production rate. (springer.com)
Despite their critical implication in worldwide public healthcare, essential and available resources such as deep transcriptome annotations remain poor, which also limits our understanding of post-transcriptional control small regulatory RNA (sRNA) functions in these bacteria. (frontiersin.org)
In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
CPEB-3 is a sequence-specific translational regulatory protein that regulates translation in a polyadenylation-independent manner. (nih.gov)

it directly interacts with the elongation factor, eEF2, to reduce eEF2/ribosome-activated GTP hydrolysis in vitro and inhibit peptide elongation of CPEB2-bound RNA in vivo. (nih.gov)

Nucleotide sequence and expression in Escherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. (microbiologyresearch.org)
Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. (microbiologyresearch.org)

RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)

5'-untranslated region (5'UTR), the hypervariable region 1 (HVR1), non-structural gene 5a (NS5a) and non-structural gene 5b (NS5b). (cdc.gov)
The average length of 5'UTR sequences has increased over evolution, suggesting that their utilization contributes to the complexity of translational regulation and organism speciation. (illinois.edu)

The full genome was sequenced, and closest similarity was found to EILV. (microbiologyresearch.org)
Due to high sequence similarity, members in this subfamily may share similar expression patterns and functions. (nih.gov)
Sequence analysis showed high similarity between mink and human sequences on corresponding farms. (cdc.gov)

So after transcription RNA has to undergo through um a few various processing steps before translation can occur because the M. RNA when it's trans transcribed is not at all ready to be translated. (pearson.com)
Immunoglobulin gene transcription is activated by downstream sequence elements. (wikidata.org)

Sequence-specific, single primer amplification and detection of PCR products for identification of hepatitis viruses. (cdc.gov)
C. Some of the viruses to be studied have been sequenced or are in process via the MRTPCR based pipeline. (jcvi.org)
Similar to other RNA viruses, HEV builds a so-called mutant cloud, which represents an intra-host heterogeneous population, with the advantage of rapid adaptation to environmental conditions 19 . (nature.com)
NNS RNA viruses also use the sequence as a template to synthesize leader RNAs (leRNAs) with unknown functions. (elsevierpure.com)
Sialokinin is unique within the insect world as having a vertebrate-like tachykinin sequence and is absent from Anopheles mosquitoes, which are incompetent for most arthropod-borne viruses, whose saliva was not proviral and did not induce similar vascular permeability. (bvsalud.org)

2006. Sequencing for quasispecies analysis was done using the method described by Ramachandran et al. (cdc.gov)
0 denotes a consensus sequence with no quasispecies analysis. (cdc.gov)
Evolutionary turnover may cause inconsistencies between sequence orthology and functional homology that substantially limit this type of analysis. (biorxiv.org)
Focusing on HIV-1, the variant responsible for the vast majority of HIV infections, the researchers have now developed a new technology called Functional Analysis of RNA Structure. (europa.eu)
As seen with many other bacteria, RNA-seq technology has now become the method of choice for transcriptome analysis in the enterococcus. (frontiersin.org)
8] when using sequence analysis of human colorectal cells. (scirp.org)
Marklewitz M , Zirkel F , Kurth A , Drosten C , Junglen S . Evolutionary and phenotypic analysis of live virus isolates suggests arthropod origin of a pathogenic RNA virus family. (microbiologyresearch.org)
In this study, formalin-fixed paraffin-embedded tissues from a previous experiment in which KASV infection was confirmed in 18 experimentally infected ERBs were used for an in-depth analysis using histopathology, in situ hybridization (ISH) for detection of viral RNA, immunohistochemistry (IHC) to assess the mononuclear phagocyte system response, and quantitative digital image analysis to investigate virus clearance from the liver and spleen within a spatial context. (cdc.gov)
Sequence analysis of new coronavirus (CoV). (cdc.gov)

When it comes to RNAi in mammals, one Ago protein in particular - Ago2 - has been shown to be a critical component of the RNA-induced silencing complex as it is the only member of this family that is capable of target mRNA cleavage. (genomeweb.com)
It can directly bind to the 3'-untranslated region of the target mRNA through its seed sequence and inhibit the expression of the encoded protein at the post-transcriptional level [3]. (scirp.org)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)

The third class of thermosensing is based on the temperature-dependent conformation of specific RNA sequences, which are termed RNA-thermometer (RNAT). (springer.com)

They contain an N-terminal unstructured region, followed by two RNA recognition motifs (RRMs), also termed RBDs (RNA binding domains) or RNPs (ribonucleoprotein domains), and a Zn-finger motif. (nih.gov)

By using ribosome profiling, the research team examined the timing of ribosome binding in relation to peak protein and RNA levels. (eurekalert.org)
These uORFs were associated with reduced ribosome binding in the main coding sequence and reduced reporter expression in a variety of circadian assays tested by the researchers suggesting a role for uORFs in shaping circadian protein expression. (eurekalert.org)
These sequences typically include the 5′-untranslated region in mRNA including the ribosome binding site (Shine-Dalgarno sequence) and in some cases the start codon (Klinkert and Narberhaus 2009 ). (springer.com)
In all organisms, translation is catalyzed in the ribosome, an extraordinary RNA-protein molecular machine in every living cell. (illinois.edu)
The RNA has one open reading frame, preceded by a ribosome-binding site within a UTR (untranslated region). (biomedcentral.com)

To address the mechanism, transcriptome-wide RNA-binding profiles of NXF1 and SRSF1-7 were determined in parallel by individual-nucleotide-resolution UV cross-linking and immunoprecipitation (iCLIP). (uni-frankfurt.de)
While there have been impressive technical innovations demonstrating feasibility-for example, generating paired measurements of single-cell transcriptome (single-cell RNA sequencing [scRNA-seq]) and chromatin accessibility (single-cell assay for transposase-accessible chromatin using sequencing [scATAC-seq])-widespread application of joint profiling is challenging due to its experimental complexity, noise, and cost. (stanford.edu)

HCV is a small (50 nm) single-stranded RNA member of the flaviviridae family. (biomedcentral.com)

The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)

G variation, located in the 3′-untranslated region, could be associated with low transcript abundance. (aspetjournals.org)
More recently, another group of researchers reported that they had developed a process for optimizing low- abundance RNA, by combining aRNA amplification with template- switching. (genomicglossaries.com)

albopictus cell lines, reporter-based strategies for assessing the ability of two types of small RNAs to inhibit a chikungunya virus (CHIKV) derived target. (bvsalud.org)

Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
Because these flies are so small, we also extracted DNA and RNA of whatever organisms were living inside of sampled individuals. (lu.se)

RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)

A) Partial alignment of 5′ untranslated region of AHFV. (cdc.gov)

RNA-Seq offers the potential for substantially improved gene annotations and for the identification of posttranscriptional RNA modifications, such as alternative splicing and RNA editing. (biomedcentral.com)

Sequence variations that affect CYP2A13 expression may contribute to interindividual differences in susceptibility to tobacco-related tumorigenesis. (aspetjournals.org)
To avoid potential confounding by sample-to-sample differences in the quality and yield of RNA preparations, or by interindividual differences in trans -acting factors that influence the expression of CYP2A13 , we determined the relative expression of variant and wild-type (WT) CYP2A13 alleles in RNA samples from heterozygotes. (aspetjournals.org)
Moreover, high throughput analyses have shown that the majority of the mammalian genome is differentially and dynamically transcribed during development to produce tens if not hundreds of thousands of short and long non-protein-coding RNAs that show highly specific expression patterns and subcellular locations. (blogspot.com)

The HCV RNA was isolated and sequenced either to determine the genotype of the isolated virus or to determine the viral quasispecies diversity within an infected individual. (cdc.gov)
Therefore, to capture the heterogeneity of the HEV quasispecies, including recombination events and SNP that occur with very low frequencies, we developed new sequencing approaches for HEV genotype 3 based on the amplification of the near full-length genome of HEV by long-range PCR (lrPCR) followed by subsequent next-generation sequencing (NGS) and third-generation sequencing. (nature.com)

CPEB-4 is an RNA-binding protein that mediates meiotic mRNA cytoplasmic polyadenylation and translation. (nih.gov)

Here, we reconstitute accelerated and selective deadenylation of RNAs containing AU-rich elements (AREs) and Pumilio-response elements (PREs). (elifesciences.org)

A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)

There are no internal repeats in the amino acid sequence of TMEM212. (wikipedia.org)

Aided by FARS-seq, the team comprehensively identified sequences and structures within the 5′ untranslated region of HIV-1 messenger RNA that regulate the equilibrium between the two conformations. (europa.eu)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)

More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)

Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
So here's the coding sequence of the M. R. N. A. We have the cap here we have the poly A tail. (pearson.com)
And we'll talk about these um and other videos but just know that here's the cap, here's the coding sequence and here's the poly A tail. (pearson.com)

This rate is patterns, gene sequencing, immuno- Centre for Disease Prevention and higher than that of severe acute respira- genicity and the animal-human and hu- Control (ECDC) and the National In- tory syndrome coronavirus (SARS), man-human interfaces. (who.int)

it can apparently be used to package therapeutic RNA molecules and deliver them to cells. (labroots.com)

Interestingly, SRSF3 and SRSF7 were shown to bind different sites in last exons and regulate 3' untranslated region length in an opposing manner. (uni-frankfurt.de)

Population-based Sanger sequencing has been shown to miss minor variants with a frequency below 20% 20 . (nature.com)

Called FARS-seq for short, it investigates the sequences and structures in HIV-1 that play an important role in dimerisation and genome packaging. (europa.eu)

2021). Serum Long Non-Coding RNA CCAT2 is a Potential Diagnostic and Prognostic Marker for Gastric Cancer. (techscience.com)

Anatomy16

Organisms28

Diseases2

Chemicals and Drugs102

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes93

Disciplines and Occupations1

Information Science4

Proteins16

Genomes9

Coding region4

MRNAs12

Polymerase4

Regions15

Genes9

Nucleotides5

Gene expression7

UTRs1

MicroRNAs1

Degradation5

MiRNA4

Genome sequence7

Splice Sites1

Genomic3

Whole genome3

Specificity1

Mutations2

Replication6

Regulatory4

Vitro1

Nucleotide sequence2

Complementary sequences1

5'UTR2

Similarity3

Transcription2

Viruses5

Analysis9

Target mRNA4

Conformation1

Motifs1

Ribosome5

Transcriptome2

Single-stranded1

Regulators1

Abundance2

Inhibit1

Organisms2

Intron1

Alignment1

Annotations1

Expression3

Genotype2

Cytoplasmic1

Selective1

Binds1

Amino1

Messenger2

MRNA sequence1

Poly3

Patterns1

Molecules1

Bind1

Sanger1

Structures1

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