Sequence genes encoding r ampicillin resistance. Medical search. Frequent questions

Insertional inactivation is a technique used in recombinant DNA engineering where a plasmid (such as pBR322) is used to disable the expression of a gene. (wikipedia.org)
Plasmid DNA exposed to saliva for 24h was still capable of transforming E. coli to ampicillin resistance, but at low efficiency: 20 cfu (colony forming units) per ml compared with 1.6 x103 cfu per ml after 24h in sterile water. (gmwatch.org)
The nptII gene in transgenic potato plants coding for kanamycin resistance, transforms naturally competent cells of the soil bacteria Pseudomonas stutzeri and Acinetobacter BD413 (both harboring a plasmid with an nptII gene containing a small deletion (hence nonfunctional) with the same high efficiency as nptII genes on plasmid DNA ( 3x10-5 -1x10-4) despite the presence of a more than 106 fold excess of plant DNA. (gmwatch.org)
A pCb plasmid encoding a beta-lactamase from Haemophilus ducreyi was transferred to Escherichia coli, purified, and characterized. (lu.se)
Moreover, several genes involved in siderophore biosynthesis (ybt, iro, and iuc), bacteriocins (clb), and capsule hyperproduction (plasmid-borne rmpA [prmpA] and prmpA2) were found, which is congruent with the positive string test displayed by K-2157. (bvsalud.org)
The CRL resides within a novel Tn2610-like hybrid Tn1721/Tn21 transposon on an IncF, ColV plasmid (pSDJ2009-52F) of 138 553 bp that encodes virulence associated genes implicated in life-threatening extraintestinal pathogenic E. coli (ExPEC) infections. (pacb.com)
Notably, pSDJ2009-52F shares high sequence identity with pSF-088-1, a plasmid reported in an E. coli ST95 strain from a patient with blood sepsis from a hospital in San Francisco. (pacb.com)
Plasmid-based expression systems for IPS and IMP were optimized, and expression of the gene zwf was regulated to enhance the stoichiometric yield of inositol. (biomedcentral.com)
Plasmid sequence and annotations. (addgene.org)
Use text editor or plasmid mapping software to view sequence. (addgene.org)
A DNA fragment encoding eGFP and also a consensus Kozak sequence (GCCGCCATGG) [14] was obtained by PCR with adaptor primers and the pEGFP-C2 plasmid (Clontech, Mountain View, CA) as a template after which cloned in to the polylinker area of p1.1 and p1.2 vectors, thereby resulting in p1.1(EBVTR-)eGFP, p1.1eGFP, p1.2HygroeGFP, p1.2-NeoeGFP and p1.2-ZeoeGFP expression plasmids. (dna-pkinhibitor.com)
Purified plasmids for transfection along with the handle plasmid pEGFP-N2 (Clontech) have been prepared applying an EndoFree Plasmid Maxi Kit (Qiagen, Valencia, CA). For stable cell line generation all plasmids except p1.2-HygroeGFP had been linearized by restriction with PvuI by cutting inside the ampicillin resistance gene bla sequence. (dna-pkinhibitor.com)
The plasmid p1.2-HygroeGFP was restricted with BspHI, which introduced two breaks close to the bla gene.Cell cultureFragments corresponding towards the upstream and downstream flanking regions (8532?2603 and 14545?8794 sequences of [GenBank:AY188393]) of your CHO elongation element 1 gene have been obtained by PCR using CHO DG44 cell (Invitrogen) genomic DNA as a template. (dna-pkinhibitor.com)
Several recent studies have indicated that plasmid-mediated resistance mechanisms also play a significant role in fluoroquinolone resistance, and its prevalence is increasing worldwide. (biomedcentral.com)
The isolates were screened for the plasmid-mediated quinolone resistance genes of qnrA , qnrB , and qnrS by PCR. (biomedcentral.com)
This report on transferable fluoroquinolone resistance due to the qnr gene among E. coli and K. pneumoniae strains indicated that plasmid-mediated quinolone resistance has emerged in pediatric patients in China. (biomedcentral.com)
qnrA is the plasmid-mediated quinolone resistance gene encoding a 218 amino acid protein of the pentapeptide family that protects DNA gyrase from quinolone inhibition[ 4 ]. (biomedcentral.com)
The insulin gene from humans was inserted into a plasmid. (pressbooks.pub)
In this example, the human insulin gene is inserted into a bacterial plasmid. (pressbooks.pub)
They isolated an antibiotic resistance gene (piece of DNA) from a plasmid of Salmonella typhimurium. (dkgoelsolutions.com)
The first report of plasmid-encoded β-lactamase capable of hydrolysing the extended-spectrum cephalosporins was published in 1983. (ajlmonline.org)
Larger plasmid carry gene for special enzymes specific for plasmid replication. (biotechfront.com)
DNA sequence which encode initiation of plasmid replication by recruiting bacterial transcription machinary for replication enzymes & proteins. (biotechfront.com)
these genes provide a survival advantage to the bacterial host thet allows for selection of plasmid containing bacteria. (biotechfront.com)
It is used as an initiation point for PCR amplification or sequencing of the plasmid. (biotechfront.com)
This plasmid carry genes that codes for colicins proteins that kill other bacteria. (biotechfront.com)
This types of plasmid carry genes that allow the host bacterium to metabolize unusual substances like Toluene and salicylic acid. (biotechfront.com)
A. Conjugative plasmid - This plasmid contain tra gene for conjugation and transfer of plasmid to another bacterium (tra+, mob+). (biotechfront.com)
This is an intermediate class of plasmid carry only a subset of genes (mob+) required for transfer. (biotechfront.com)
The plasmid pGLO contains an antibiotic-resistance gene, ampR, and the GFP gene is regulated by the control region of the ara operon. (bartleby.com)
The E. coli then transforms the pBLu plasmid, which carries the genes coding for two identifiable phenotypes. (bartleby.com)
We conclude that cephalosporin resistance among E. coli O157:H7 is due to plasmid- encoded bla CMY genes and that plasmid types appear to have shifted from IncA/C to IncI1. (cdc.gov)
KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)

Because the sequence of the transposon is known, the gene can be identified, either by sequencing the whole genome and searching for the sequence, or by using the polymerase chain reaction to amplify specifically that gene. (wikipedia.org)
Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and blaROB-1 encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. (edu.au)
Whole-genome sequencing (WGS) and hybrid assembly were performed, using data generated on the Illumina and Nanopore platforms. (bvsalud.org)
Screening of Antibiotic and Virulence Genes from Whole Genome Sequenced Isolated from Food and Milk-Producing Environments. (ccc.ac.at)
The objective of this study was to use whole-genome sequencing (WGS) to screen for genes encoding for antibiotic resistance, fitness and virulence in strains that had been isolated from food and powdered-milk-producing environments. (ccc.ac.at)
Using specific DNA barcodes and whole-genome sequencing, population dynamics were followed, revealing the impact of individual Rap phosphatases and arising mutations on the adaptability of B. subtilis . (nature.com)

An example is the use of pBR322, which has genes that respectively encode polypeptides that confer resistance to ampicillin and tetracyclin antibiotics. (wikipedia.org)
The integron abuts a copy of Tn6029, an IS26-flanked composite transposon encoding blaTEM, sul2 and strAB genes that confer resistance to ampicillin, sulfathiazole and streptomycin, respectively. (pacb.com)
Enterobacteriaceae with resistance to third-generation cephalosporins carry genes encoding extended-spectrum β-lactamase (ESBL) production, which also confer resistance to penicillins and monobactams. (biomedcentral.com)
Plasmids are genetic elements that mobilize antimicrobial resistance determinants including bla CMY -lactamases that confer resistance to extended-spectrum cephalosporins (ESC). (cdc.gov)

Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. (edu.au)
Alarmingly, isolates displaying a convergence phenotype of carbapenem resistance and hypervirulence have been detected in several countries, representing a serious threat to public health. (bvsalud.org)
Enterobacterales isolates that are concomitant ESBL producers and are carbapenem resistant have been increasingly reported and demonstrate alarmingly increased antibiotic resistance patterns compared with ESBL Enterobacterales . (frontiersin.org)
Three % of the ampr isolates from the rectal samples yielded positive results when screened for the presence of blaTEM genes by PCR. (uit.no)
ABSTRACT The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the blaKPC gene that encodes for carbapenemase production have complicated the management of patient infections. (who.int)
This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)
The blaKPC gene was confirmed in 14/20 (70.0%) of these isolates. (who.int)
The high prevalence of ertapenem nonsusceptibility at a tertiary care hospital in Egypt was predominantly attributed to K. pneumoniae carbapenemase-mediated resistance mechanisms in K. pneumoniae isolates. (who.int)
In China, the presence of the qnr gene in the clinical isolates of Enterobacteriaceae has been reported, but this transmissible quinolone resistance gene has not been detected in strains isolated singly from pediatric patients. (biomedcentral.com)
This study therefore aimed to investigate the presence of the qnr gene in clinical isolates of E. coli and K. pneumoniae from pediatric patients in China. (biomedcentral.com)
The study found that 19 ciprofloxacin-resistant clinical isolates of E. coli and K. pneumoniae were positive for the qnr gene, and most of the qnr positive strains were ESBL producers. (biomedcentral.com)
Therefore, the objective of this study was to screen for the presence of the qnr gene in clinical ciprofloxacin-resistant isolates of Escherichia coli and Klebsiella pneumoniae from pediatric patients in China. (biomedcentral.com)
MLST analysis showed that 32 sequence types (STs) were identified, while ST46 was the predominant genotype among all isolates. (hindawi.com)
Genome sequence analysis of 20 S . ruminantium isolates provided information about the antibiotic resistance, potential virulence, and serological diversity of this species. (biomedcentral.com)
The identified isolates were tested by Coagulase gene RFLP typing. (hindawi.com)
The aims of this research work were to determine the patterns of antibiotic resistance in Escherichia coli isolates from the meat of wild or domestically reared pigeons from Spain, to detect the presence of virulence and antibiotic resistance genes, and to carry out a phylogenetic classification of the isolates. (mdpi.com)
More analysis of human, retail meat, and food animal isolates is necessary to broaden our understanding of the antimicrobial resistance determinants of ESC resistance among E. coli O157. (cdc.gov)
One hundred eight isolates from both substrates were identified by amplified ribosomal DNA restriction analysis and/or 16S rRNA gene sequencing and were determined to belong to the following 11 species from the genera: Lactococcus, Leuconostoc, Lactobacillus (L.), and Oenococcus. (who.int)

E. coli strain DH5a was the test micro-organism for horizontal gene transfer. (gmwatch.org)
Two nucleotides were different between the E. coli (Tn3) and H. ducreyi (pCb) genes that affected the amino-acid sequence. (lu.se)
However, the yields of correctly folded, functional antibody fragments in E. coli are often relatively low and dependent on the type and primary sequence of the fragment. (biomedcentral.com)
Since the disulfides cannot be efficiently formed in the reducing cytoplasm of E. coli , antibody fragments are most commonly supplemented with a signal sequence that directs them to the more oxidizing bacterial periplasm for correct folding. (biomedcentral.com)
In this system, recombinase variants are selected for activity on new substrates based on enzyme-mediated reassembly of the gene encoding beta-lactamase that confers ampicillin resistance to Escherichia coli. (duke.edu)
Phenotypic tests confirmed the array-based typing results and corroborate that the selected probes allowed correct typing and prediction of major antibiotic resistances of clinically relevant Enterobacteriaceae , including the subspecies level, e.g. the reliable distinction of different E. coli pathotypes. (biomedcentral.com)
Generally, uropathogenic E. coli (UPEC), newborn meningitis-associated E. coli (MNEC) as well as sepsis-associated E. coli (SEPEC) differ in their repertoire of virulence-associated genes from IPEC [ 1 ]. (biomedcentral.com)
Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
Sequence type 58 (ST58) phylogroup B1 Escherichia coli have been isolated from a wide variety of mammalian and avian hosts but are not noted for their ability to cause serious disease in humans or animals. (pacb.com)
Here we determined the genome sequences of two multidrug-resistant E. coli ST58 strains from urine and blood of one patient using a combination of Illumina and Single Molecule, Real-Time (SMRT) sequencing. (pacb.com)
In all the samples, 50 E. coli strains were isolated and then assigned to serotyping, antimicrobial susceptibility test, detection of antimicrobial resistance genes and the Class 1 integrons, and multilocus sequence typing (MLST). (hindawi.com)
Our results showed that farmed minks could be reservoirs of antimicrobial-resistant E. coli with Class 1 integron cassettes and resistance genes, which were likely to pose a threat to public health. (hindawi.com)
Therefore, continuous inspections and monitoring of E. coli in minks are essential for detecting and controlling emerging E. coli with different serovars as well as antibiotic resistance. (hindawi.com)
The level of antibiotic resistance among pathogenic and commensal E. coli has steadily increased and has become a global health concern [ 7 , 8 ]. (hindawi.com)
A previous study also demonstrated that E. coli can carry resistance plasmids and can easily acquire resistance transfer genes [ 9 , 10 ]. (hindawi.com)
533 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. (novoprolabs.com)
As a result, the gene was expressed & multiplied in E. coli. (dkgoelsolutions.com)
E. coli was artificially induced that became a competent bacteria when it took the pGLO DNA, so it had the ability to have ampicillin resistance and fluoresced when arabinose was present. (bartleby.com)
The results showed only one E. coli culture had growth and fluoresced which was the pGLO+ E. coli that was grown on the plate with LB, amp +arab, there were only two E. coli cultures that did not grow because the pGLO- E. coli did not have the ampicillin resistance to grow in ampicillin conditions, and the rest of the culture plates showed growth. (bartleby.com)
Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. (bartleby.com)
Thus, transformed E. coli cells containing ampicillin-resistance plasmids can easily be selected simply growing the bacteria in the presence of ampicillin-only the transformed cells survive. (bartleby.com)
generated 250 million E. coli with either random or targeted changes to the gene that codes for the NfsA enzyme. (elifesciences.org)

Hence, when a genetic region is interrupted by the integration of pBR322, the gene function is lost but new gene function (resistance to specific antibiotics) is gained. (wikipedia.org)
The beta-lactamase could hydrolyze both penicillin antibiotics including ampicillin, benzylpenicillin, and carbenicillin as well as cephalosporin antibiotics including nitrocefin, cephalothin, cephaloridine, and cefoperazone. (lu.se)
This pathogen is characterized by its notorious resistance to last-line antibiotics, such as carbapenems. (bvsalud.org)
In Enterobacterales , the prime antimicrobial resistance mechanism against β-lactam antibiotics is mainly the production of β-lactamases, particularly extended-spectrum β-lactamases (ESBLs). (frontiersin.org)
This plasmids carry genes thet provide resistance to one or more antibiotics and poisons. (biotechfront.com)
Monitoring resistance to antibiotics in wild animals may assist in evaluating tendencies in the evolution of this major public health problem. (mdpi.com)

In addition, K-2157 harbored two plasmids: one of 113,644 bp (KPC+) and another of 230,602 bp, containing virulence genes, in addition to an integrative and conjugative element (ICE) embedded on its chromosome, revealing that the presence of these mobile genetic elements mediates the convergence between virulence and antibiotic resistance. (bvsalud.org)
Plasmids p1.2 were transfected by electroporation in Gene Pulser Electroporation Buffer (Bio-Rad, Hercules, CA) making use of a P2X1 Receptor Antagonist Molecular Weight cuvette using a four mm gap with 7.5 million cells and 15 g of linearized DNA for every transfection. (dna-pkinhibitor.com)
The acquisition of resistance genes through a mechanism involving mobile genetic elements, such as plasmids and transposons, is considered a major contributor to antimicrobial resistance [ 9 , 10 ]. (hindawi.com)
but genes carried by plasmids usually encodes traits beneficial for bacteria. (biotechfront.com)

Results A 16S rRNA gene clone library was constructed, and all sequences obtained from 161 clones showed affiliation with the phylum Firmicutes, with 160 sequences identified as Clostridiales and one sequence identified as unclassified Firmicutes. (uit.no)
Citrobacter gillenii was isolated from the diseased fish and identified by biochemical and molecular methods including API 20E and 20NE and 16S rRNA gene region sequencing, respectively. (iranjournals.ir)

Amino acid sequencing of some beta-lactamases has shown that substitution of only a few amino acids in the bla gene leads to high-level resistance against specific cephalosporins. (lu.se)
ESBL-producing Enterobacterales from the GCC region show high levels of resistance to ampicillin, aztreonam, third-/fourth-generation cephalosporins, fluoroquinolones, and trimethoprim-sulfamethoxazole. (frontiersin.org)

Little is known of their gut microbial ecology and the main purpose of this study was to investigate the microbial diversity in faeces of polar bears in Svalbard, Norway (74-81 degrees N, 10-33 degrees E). In addition the level of blaTEM alleles, encoding ampicillin resistance (ampr) were determined. (uit.no)
1228 /gene="bla" /label=AmpR promoter CDS 1229. (novopro.cn)

Enteritidis, Japan tamycin, sulfamethoxazole-trimetho- was hybridized to the probe for ampi- prim, trimethoprim, chloramphenicol, cillin resistance described above. (cdc.gov)
L tubes and centrifuged at 5,000 rpm for 5 s and spread onto an LB plate containing ampicillin and chloramphenicol. (billfryer.com)
Producing hydroquinones is helpful to the cell in some situations, but if bacteria are regularly exposed to chloramphenicol, NfsA's role aiding antibiotic resistance could become more important. (elifesciences.org)
Additionally, the experiments showed that the bacteria with improved resistance to chloramphenicol also became more sensitive to another antibiotic, metronidazole. (elifesciences.org)

Although QACs may play an important role in the propagation of antibiotic resistance gene (ARGs), the potential contribution and mechanism remains unclear. (bvsalud.org)

Insect-resistant maize line CG00526-176 contains three bacterial genes: the cry1A(b) specific to lepidopterans, the bar gene conferring tolerance to glufosinate, and a bla gene encoding TEM-1 b-lactamase (ampicillin resistance). (gmwatch.org)
bla CTX-M (subtype group 1) followed by/co-dominated by bla TEM and bla SHV , whereas the most common carbapenem-resistant genes are bla OXA-48 and bla NDM-1 . (frontiersin.org)
Almost all (93%) strains were resistant to cephalotin and 33% were resistant to ampicillin. (ccc.ac.at)
The current study aimed to use Coagulase gene polymorphism to identify methicillin-resistant Staphylococcus aureus (MRSA) subtypes isolated from nasal carriers in Minia governorate, Egypt, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. (hindawi.com)
Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)

The evidence for horizontal gene transfer is accumulating. (gmwatch.org)
The researchers investigated the survival of DNA from transgenic maize and the transfer of the antibiotic resistance bla gene to bacteria in the presence of saliva, rumen fluid and silage effluent, which are relevant to horizontal gene transfer in the oral cavity, the rumen, and in silage. (gmwatch.org)
In summary, horizontal gene transfer can occur before the DNA is completely broken down, even when the breakdown is rapid, as in the rumen or in silage. (gmwatch.org)
DNA breakdown is extremely slow in saliva, and hence the oral cavity will be a very important site for horizontal gene transfer. (gmwatch.org)
Our approach circumvents misclassifications arising from the application of virulence markers, which are highly affected by horizontal gene transfer. (biomedcentral.com)
Recall that these mechanisms are examples of horizontal gene transfer -the transfer of genetic material between cells of the same generation. (pressbooks.pub)
The earliest variants of ESBLs originated as a result of point mutations in the genes for broad-spectrum β-lactamases whilst newer ones, including the most successful such as CTX-M, arose by acquisition from the environmental metagenome through horizontal gene transfer. (ajlmonline.org)

Restriction endonuclease recognizes a specific palindromic nucleotide sequences in the DNA. (dkgoelsolutions.com)

As a result, the inventors discovered several genes that were highly expressed specifically in lactic acid-producing yeasts, and isolated those promoters. (justia.com)

Antibiotic resistance genes along with the SV40 promoter and terminator regions were obtained by amplification with adaptor primers, employing pcDNA3.1/Hygro, pcDNA3.1(+), and self-ligated pcDNA4/HisMax-TOPO (Invitrogen) as PCR templates. (dna-pkinhibitor.com)

Next, we injected in vitro synthesized mRNA encoding green fluorescent proteins (GFP) and noticed its expression in every effectively injected embryos (embryos are amenable to microinjection. (careersfromscience.org)

Commensal bacterial populations belonging to Bacteroidetes most commonly possessed ESBL genes followed by members of Enterobacteriaceae. (biomedcentral.com)
Antibiotic-treated cattle had an increased abundance of specific taxa and genes encoding ESBL production that persisted for 9 weeks. (biomedcentral.com)

Sleeping Beauty, is designed to interrupt a gene in such a way that it causes maximal genetic havoc. (wikipedia.org)
The genomic features of K-2157 (e.g., sequence type, K locus, and mobile genetic elements) were retrieved using different bioinformatic tools. (bvsalud.org)
The engineering of new enzymes that efficiently and specifically modify DNA sequences is necessary for the development of enhanced gene therapies and genetic studies. (duke.edu)
Genetic engineering involves the use of recombinant DNA technology, the process by which a DNA sequence is manipulated in vitro, thus creating recombinant DNA molecule s that have new combinations of genetic material. (pressbooks.pub)
Moreover, the contig and network analyses detected associations between β-lactam resistance genes and phages, mobile genetic elements, and specific genera. (biomedcentral.com)
In genetic engineering, only desirable genes are introduced. (dkgoelsolutions.com)

Quinolone resistance in Enterobacteriaceae results mainly from mutations in type II DNA topoisomerase genes and/or changes in the expression of outer membrane and efflux pumps. (biomedcentral.com)
1 Since then, extended-spectrum β-lactamases (ESBLs) have become an increasingly important resistance mechanism among Enterobacteriaceae worldwide. (ajlmonline.org)

This viral gene insertion is also known as a promoter insertion as it drives the expression of the c-myc gene. (wikipedia.org)
A newly developed β-lactamase (ΔW290) selection cassette contains a segment inside the β-lactamase open reading frame encoding a stretch of hydrophilic amino acids that result in a T7 promoter when back-translated. (biomedcentral.com)
62 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 69. (novoprolabs.com)
345 /note="Xenopus globin 3'-UTR" /note="3'-UTR of the major beta-globin gene of Xenopus laevis" promoter complement(457. (novoprolabs.com)
The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts. (justia.com)
The present invention relates to promoter DNAs in the presence of organic acids, DNA constructs, transformants, expression methods of a recombinant gene, and their uses in organic acid production methods. (justia.com)
The inventors have already established a system to express an L-lactate dehydrogenase gene using an original promoter function of the foreign PDC1 gene and exclude the expression of PDC1 protein that is expressed by the original foreign promoter, by linking the objective valuable gene (L-lactate dehydrogenase gene in this case) in the downstream of chromosomal PDC1 promoter and disrupting the yeast chromosomal PDC1 gene simultaneously. (justia.com)
Although such a stable high-level gene expression system has been established, a promoter with strong expression potency is needed to further increase the productivity of recombinant products. (justia.com)
The yeast promoters published include alcohol dehydrogenase 1 gene (ADH1) promoter (J Ferment Bioeng, 1998, Vol. 8 6(3) pp 284-289), triose phosphate isomerase gene (TPI1) promoter (FEMS Microbial Lett, 1999, Feb. Vol. 171 (2) pp 133-140), and the like. (justia.com)
Thus, the promoter that activates the transcription of operatively-associated DNA in the presence of an organic acid is useful to increase the objective gene product in the presence of an organic acid, in particular, those are useful to increase the objective product during the production of an organic acid. (justia.com)
Therefore, such a promoter can be used to increase the production of organic acids by manipulatively associating protein genes involved in organic acid production. (justia.com)

Both ST58 strains were clonal and were characterised as serotype O8:H25, phylogroup B1 and carried a complex resistance locus/loci (CRL) that featured an atypical class 1 integron with a dfrA5 (trimethoprim resistance) gene cassette followed by only 24 bp of the 3'-CS. (pacb.com)

Also, ampC, sul1 and floR resistance genes were detected in the isolated bacteria. (iranjournals.ir)
AmpC -lactamases, commonly encoded by bla CMY-2 genes [11]. (cdc.gov)

The discriminative power of the probe set was further tested in silico on 15 additional complete enterobacterial genome sequences. (biomedcentral.com)

Strains sensitive to all the antimi- resistance determinant. (cdc.gov)
a series of engineered strains for inositol production was constructed by deleting the key genes pgi , pfkA and pykF . (biomedcentral.com)
The aim of this study was to use coa gene polymorphism to identify MRSA subtypes isolated from nasal carriers in Minia governorate, Upper Egypt, evaluate the efficiency of these typing methods in discriminating variable strains, and compare these subtypes with antibiotypes. (hindawi.com)

It confirmed that the enzyme belonged to a class A beta-lactamase which had 99% identity to the ampicillin resistance transposon Tn3 of pBR322. (lu.se)

In most gram-positive bacteria, this operon consists of five genes dltXABCD but the involvement of the first ORF ( dltX ) encoding a small protein of unknown function, has never been investigated. (frontiersin.org)
We, therefore constructed an in frame deletion mutant of dltX , without affecting the expression of the other genes of the operon. (frontiersin.org)

The bla gene originates from the cloning vector PUC18 and is not expressed in maize, but has bacterial regulatory sequences that would allow it to become functional were it to be transferred back into bacteria. (gmwatch.org)
A positive selection for the insert results whenever the cloning leads to the creation of an additional resistance. (biomedcentral.com)
The term 'negative selection' is used here to describe cloning systems with vectors that contain the ccdB cell-death gene which is replaced by the cloned insert, i.e . the selection eliminates the vector molecules without insert. (biomedcentral.com)
LOCUS Exported 3029 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pFH2191, complete sequence. (novopro.cn)

At week 5, the antibiotic-treated cows had lower microbiota abundance and richness, whereas a greater abundance of genes encoding extended-spectrum β-lactamases (ESBLs), CfxA, ACI-1, and CMY, was observed at weeks 1, 5 and 9. (biomedcentral.com)

509) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 517. (novoprolabs.com)

The escalating global prevalence of MDR in Enterobacterales has led to limited treatment options, raising an urgent need for novel antimicrobial therapy(s) and detailed studies exploring underlying resistance mechanisms. (frontiersin.org)
Because the entire genome of a laboratory strain of H influenzae has been sequenced, additional insights into its molecular mechanisms of pathogenesis will undoubtedly be gained in the near future. (medscape.com)
The K27M and G34R mutations induced several of the same pathways suggesting key shared oncogenic mechanisms including activation of neurogenesis and NOTCH pathway genes. (nature.com)
Taken together, our findings suggest striking overlap between putative mechanisms of K27M and G34R mutations and point to increased NOTCH signaling playing a key role in K27M-related gliomagenesis, resulting from aberrant gene derepression due to impaired H3K27me3 deposition at super-enhancers in H3.3 mutant cells. (nature.com)
There are many resistance mechanisms underlying the emergence and prevalence of antimicrobial resistance. (hindawi.com)
By identifying and characterizing the mechanisms of resistance and how they are spread, we hope to better understand the relationships between resistance attributes and possible sources of human infection among food animals and their retail meat. (cdc.gov)

BACKGROUND: Non-typhoidal Salmonella (NTS) is a common cause of gastroenteritis in young children, with limited data on NTS serovars and antimicrobial resistance in Africa. (cdc.gov)

Unselected prodrug-converting activities were mostly unaffected, emphasising the importance of negative selection to effect enzyme specialisation, and offering an application for the evolved genes as dual-purpose selectable/counter-selectable markers. (elifesciences.org)

Strikingly, K-2157 displayed a resistome composed of ß-lactam resistance genes (blaSHV-190, blaTEM-1, blaOXA-9, and blaKPC-2), the fosfomycin resistance gene fosA, and the fluoroquinolones resistance genes oqxA and oqxB. (bvsalud.org)

As a result, viral promotors have been replaced by cellular promotors and regulatory sequences. (wikipedia.org)

Also various molecular typing techniques have been used, including pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), and these assays were considered as efficient techniques in MRSA typing but they are time consuming, expensive, and technically complex [ 8 ]. (hindawi.com)

The use of recombinant DNA technologies is nowadays spread through most laboratories conducting research in life sciences and the applications, including gene expression systems, tend towards being more parallelized. (biomedcentral.com)
Recombinant tRNA and selection marker coding sequences are likewise provided. (justia.com)
With advances in recombinant DNA technology, techniques to obtain objective gene products have developed through expression of a foreign gene in hosts such as bacteria, fungi, animals, plants, and insects, and proliferation of the transformants. (justia.com)
For example, culture of recombinant yeasts allows production of large amounts of objective gene products by means of fermentative production. (justia.com)
Thus, an object of the invention is to provide promoters usable in the presence of an organic acid, DNA constructs having the promoters, transformants comprising the DNA constructs, expression methods of recombinant genes in the transformants, and production methods of organic acids using the transformants. (justia.com)

In some aspects the bacterial cell comprises a heterologous nucleic acid encoding a screenable marker that exhibits enhanced activity when at least one position of the marker polypeptide is said first non-canonical amino acid. (justia.com)
Kunitz-like domain"--A protease inhibitor similar to soybean trypsin inhibitor or a nucleic acid sequence encoding a protease inhibitor which is similar to the soybean trypsin inhibitor. (everypatent.com)

1. A purified protease which comprises the amino acid sequence SEQ ID NO:1, or a functional equivalent thereof produced by conservative amino acid alterations which result in a protease which exhibits the same biological, biochemical, chemical, physical and structural properties of the protease of SEQ ID NO:1. (everypatent.com)

Infections secondary to these pathogens are widely common but multidrug resistance (MDR) in Enterobacterales has become a significant challenge with increased morbidity, mortality, and cost of management. (frontiersin.org)

Here we report successful gene editing of H3.3K27M and G34R point mutations in human normal brain and pediatric glioma cells, generating pairs of otherwise isogenic cell lines that allowed us to define epigenetic and transcriptomic changes contributing to gliomagenesis in the native genomic context. (nature.com)
To study the transformative effects of oncohistone mutations in human pediatric gliomas in an otherwise isogenic context, we used CRISPR-Cas9 gene editing to revert H3.3K27M mutations in glioma cells back to wild type (WT) while in parallel introducing H3.3K27M and G34R point mutations into human astrocytes and H3.3WT glioma cells (Fig. 1a ). (nature.com)

Directed evolution of recombinase specificity by split gene reassembly. (duke.edu)
More recently, increased expression of NOTCH pathway genes was implicated in some DIPGs and NOTCH path inhibition contributed to reduced tumor growth in culture 14 , but the specificity of these treatments for H3.3 mutant cells remains unclear. (nature.com)
Within these fragments, most sequence variation is located in complementarity determining regions (CDRs), which influences antigen-binding specificity. (vectorbuilder.cn)

Insertional mutagenesis is possible whether the virus is of the self-inactivating types commonly used in gene therapy or competent to replicate. (wikipedia.org)

VectorBuilder's mammalian antibody heavy chain gene expression vector has been designed specifically for high-yield production of monoclonal antibody heavy chains. (vectorbuilder.cn)
Even when grown in liquid mixed cultures, where environmental conditions are assumed to be homogeneous, B. subtilis cells can be found as single flagellated cells or as non-flagellated chained cells due to stochastic variation in the expression of motility-related genes 3 . (nature.com)
To achieve high productivity of the objective substance from recombinants, a stable high-level gene expression system must be established. (justia.com)
The inventors have disclosed in Japanese Published Unexamined Patent Application 2003-164295 that the use of this system overcomes conventional difficulties and allows stable high-level gene expression. (justia.com)
The inventors obtained mRNA from lactic acid-producing yeasts or yeasts growing in lactic acid-containing medium and conducted thorough gene expression analysis by a quantitative PCR method. (justia.com)
Then, to disrupt the PDC1 gene on a yeast chromosome, an expression cassette having a L-lactate dehydrogenase gene in the downstream of these promoters was integrated into the PDC1 gene locus that had previously been mapped to the chromosome, and the production of large amounts of L-lactic acids was confirmed. (justia.com)

In a first embodiment the invention provides a transgenic bacterial cell, said cell comprising heterologous nucleic acids encoding translation components for incorporation of at least a first non-canonical amino acid and a screenable or selectable marker polypeptide that exhibits enhanced activity when at least one position of the marker polypeptide is said first non-canonical amino acid. (justia.com)

A mouse model study implicated alterations in bivalent genes in mutant H3.3 downstream function 20 . (nature.com)

Historically, lentiviral vectors included strong viral promoters which had a side effect of insertional mutagenesis, nuclear DNA mutations that effect the function of a gene. (wikipedia.org)
Mutations conferring resistance to the inhibitors are clustered at this binding pocket. (biorxiv.org)

Virulence (VGs) and antibiotic-resistance genes (ARGs) were detected with the Comprehensive Antibiotic Resistance Database (CARD) platform, ResFinder and PlasmidFinder tools. (ccc.ac.at)

However, if the insertion occurs in an essential gene or a gene that is involved in cellular replication or programmed cell death, the insertion may compromise the viability of the cell or even cause the cell to replicate interminably - leading to the formation of a tumor, which may become cancerous. (wikipedia.org)
however when it is turned on it is able to push the cell into the G1 phase of the cell cycle and cause the cell to begin replication, causing unchecked cell proliferation while allowing the viral gene to be replicated. (wikipedia.org)
A piece of alien DNA has no the sequence called Origin of replication (ori) needed for starting replication. (dkgoelsolutions.com)

We have tested eight vectors by inserting six target sequences of variable length, provenience and function. (biomedcentral.com)
Antibiotic resistance cassettes had been sub-cloned into T-vectors and then transferred in to the p1.2-Mono backbone by restrictionligation resulting in p1.2-Hygro, p1.2-Neo and p1.2-Zeo. (dna-pkinhibitor.com)
By customizing sequences encoding the heavy chain variable region, customers may generate vectors to satisfy their personalized need for antigen-binding specificities. (vectorbuilder.cn)

Anatomy2

Organisms12

Diseases6

Chemicals and Drugs46

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes36

Anthropology, Education, Sociology and Social Phenomena1

Information Science2

Plasmid33

Whole genome6

Confer resistance4

Isolates18

Coli22

Antibiotics6

Plasmids4

RRNA gene2

Cephalosporins2

Ampr2

Chloramphenicol4

Antibiotic resistance gene1

Resistant5

Horizontal gene tra7

Nucleotide sequences1

Several genes1

Primers1

Proteins1

ESBL2

Genetic6

Enterobacteriaceae2

Promoter11

Trimethoprim1

AmpC2

Genome sequences1

Strains3

PBR3221

Operon2

Cloning4

Extended-spectrum1

Protein1

Mechanisms6

Salmonella1

Enzyme1

BlaTEM1

Regulatory sequences1

Multilocus1

Recombinant5

Nucleic acid2

Amino-acid seq1

Multidrug resistance1

Point mutations2

Specificity3

Commonly1

Expression6

Bacterial cell1

Downstream1

Mutations2

ARGs1

Replication3

Vectors3