• Analytical techniques in clinical chemistry for the estimation of polypeptide hormone concentrations in biological fluids are changing from limited reagent, radioimmunoassay methods to excess reagent immunoradiometric methods, with a consequential improvement in sensitivity, precision and speed. (ucl.ac.uk)
  • Using the standard isotopic protocol, secretion of hGH protein into the culture-medium supernatant can be monitored approximately 24hours post-transfection, depending on the cell type and the sensitivity of the assay used. (thomassci.com)
  • Other urinary tests under development that are not, to date, recommended for diagnostic use, include UBC-Rapid/ELISA test, CYFRA 21-1 and BLCA-1/BLCA-4, which assay proteins predominantly present in metastatic cells. (encyclopedia.pub)
  • The biological activities of these different forms were determined using a sensitive and specific in-vitro bioassay developed in the course of this study. (ucl.ac.uk)
  • A RIA is a very sensitive in vitro assay technique used to measure concentrations of substances, usually measuring antigen concentrations (for example, hormone levels in blood) by use of antibodies. (wikipedia.org)
  • The same combination of monoclonal antibodies has been used to develop a more sensitive time resolved immunofluorometric assay for hFSH. (ucl.ac.uk)
  • Our current knowledge of hFSH microheterogeneity and the assays available indicates the urgent need to select antibodies with specific binding to the biological active forms of the hormone. (ucl.ac.uk)
  • Serum levels of a tumor-associated antigen, designated CA 15-3, which is defined by two monoclonal antibodies, in 60 untreated breast cancer patients in various stages were determined by immunoradiometric assay. (medicaljournal-ias.org)
  • In contrast, EVLWI is probably a sensitive marker of subclinical pulmonary edema particularly in patients with elevated left-ventricular-filling pressure irrespective of differences in left ventricular function. (springeropen.com)
  • The hGH assay system differs from most of the other commonly used reporter proteins (CAT, β-Gal, or luciferase) in one important respect: hGH is a secreted protein, and is measured using samples of culture-medium supernatants thus (i) avoiding the necessity to lyse cells, (ii) allowing continuous monitoring of transient expression kinetics, and (iii) allowing use of the cells for other purposes (e.g. (thomassci.com)
  • The most commonly used assays are for the beta subunit of human chorionic gonadotropin (hCG). (medscape.com)
  • To this end, hyperandrogenemic oligo- or anovulatory patients with polycystic ovarian syndrome (n = 11) and healthy girls (n = 9) underwent overnight blood sampling every 20 min for 12 h to monitor simultaneous secretion of leptin (immuno-radiometric assay), LH (immunofluorometry), and androstenedione and T (RIA). (elsevierpure.com)
  • These observations indicate that CA 15-3 is a sensitive tumor marker for diagnosis and especially for monitoring of breast cancer patients. (medicaljournal-ias.org)
  • As such, baseline levels in patients who undergo serial CA 125 monitoring should be redetermined if the methodology in the assay is changed. (medscape.com)
  • Immunoradiometric assay some research patients with rare percent less than vials. (queobathrooms.co.uk)
  • Haciyanli MLal GMorita EDuh QYKebebew EClark OH Accuracy of preoperative localization studies and intraoperative parathyroid hormone assay in patients with primary hyperparathyroidism and double adenoma. (jamanetwork.com)
  • A preliminarily study of carcinogenesis and chemosensitivity suggests that this cell model carries a tumor suppressor gene mutation and is sensitive to chemotherapy drugs. (oncotarget.com)
  • Standardization of hepatitis E virus (HEV) nucleic acid amplification technique-based assays: an initial study to evaluate a panel of HEV strains and investigate laboratory performance. (jctlm.org)
  • After extensive washing, the direct amount of radioactive antibody bound is measured and the amount of target molecule quantified by comparing it to a reference amount assayed at the same time. (wikipedia.org)
  • Skeletal muscle is the largest insulin-sensitive tissue in the body, and skeletal muscle insulin resistance is a well-established key process in the development of type 2 diabetes mellitus (T2DM) [ 7 ]. (e-dmj.org)
  • Currently, through the use of chemical assays and ultrasonography, physicians are capable of making the diagnosis of pregnancy before many of the physical signs and symptoms are evident. (medscape.com)
  • Although the RIA technique is extremely sensitive and extremely specific, requiring specialized equipment, it remains among the least expensive methods to perform such measurements. (wikipedia.org)
  • lag-specific cross-correlation analysis and pattern-sensitive cross-approximate entropy. (elsevierpure.com)
  • The technique is highly sensitive and specific and can detect a wide range of antigens in many animal species. (creativebiolabs.net)
  • For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. (creative-biolabs.com)
  • Carneiro DMSolorzano CCNader MCRamirez MIrvin GL III Comparison of intraoperative iPTH assay (QPTH) criteria in guiding parathyroidectomy: which criterion is the most accurate? (jamanetwork.com)
  • Their availability has allowed the development of a sensitive, non-competitive, sandwich type radioimmunoassay for human follicle stimulating hormone (hFSH). (ucl.ac.uk)
  • Commutability of the certified reference materials for the standardization of beta-amyloid 1-42 assay in human cerebrospinal fluid: lessons for tau and beta-amyloid 1-40 measurements. (jctlm.org)
  • Ahmed AH , Gordon RD , Taylor PJ , Ward G , Pimenta E , Stowasser M . Effect of contraceptives on aldosterone‐renin ratio may vary according to the components of contraceptive, renin assay method, and possibly route of administration. (comprehensivephysiology.com)
  • The assay is based on immature rat granulosa cells producing plasminogen activator in response to hFSH. (ucl.ac.uk)
  • Antineoplastic drugs only kill drug-sensitive cells. (creativebiolabs.net)
  • Enzyme Linked Immunosorbent Assay (ELISA) is the gold standard assay for detecting and identifying biomolecules using antibodies as the probe. (bvsalud.org)
  • A fluorescent enzyme-linked immunosorbent assay is described for the rapid measurement of serum ferritin. (huji.ac.il)
  • The expression of serum osteoprotegerin, as measured by enzyme-linked immunosorbent assay, has been studied in 82 patients with locally controlled prostate cancer, in 49 patients with metastatic bone disease and in a control group of 41 healthy males. (hippokratia.gr)
  • 9. Clinical relevance of highly sensitive Tg assay in monitoring patients treated for differentiated thyroid cancer. (nih.gov)
  • 12. [Recovery test or immunoradiometric measurement of anti-thyroglobulin autoantibodies for interpretation of thyroglobulin determination in the follow-up of different thyroid carcinoma]. (nih.gov)
  • The development of sensitive immunoradiometric TSH assays in the mid 1980s facilitated discrimination of these patient groups and identified individuals with subnormal TSH levels despite thyroid hormone concentrations within the normal range, [ 1 ] a biochemical entity termed subclinical thyrotoxicosis (SCT). (medscape.com)
  • The availability of sensitive and specific assays for evaluation of the thyroid axis has allowed definition of thyroid disorders at subclinical stage. (drshahpathologyendo.com)
  • Thyroid-stimulating hormone and anti-thyroid autoAb were measured by an immunoradiometric method, thyroid hormones by chromatographic assay, and non-organ-specific autoAb by indirect immunofluorescence. (nih.gov)
  • The cost of the assay is less than 10% of the immunoradiometric assay for serum ferritin. (huji.ac.il)
  • Serum total prostate-specific antigen (PSA) was determined by an immunoradiometric assay. (hippokratia.gr)
  • Sensitive assay using radiolabeled ANTIGENS to detect specific ANTIBODIES in SERUM . (nih.gov)
  • Some reports, however, have suggested that the serum GGT may be a sensitive and early biomarker for the development of prediabetes and diabetes. (jpmph.org)
  • The samples were analyzed specifically for papain utilizing a very sensitive (low nanogram) radioimmunoassay procedure. (cdc.gov)
  • Since the first description of an immunoassay method in 1959, advances have been made in assay designs and analytical characteristics, opening the door for their widespread implementation in clinical laboratories. (annlabmed.org)
  • The aim of our study was to perform the analytical evaluation of the inter-assay comparability for tumor markers CA19-9, CEA and AFP on two different automated chemistry analyzers. (biochemia-medica.com)
  • Until today, prostate-specific antigen (PSA) level represents the most sensitive tumor marker available for monitoring disease progression. (hippokratia.gr)
  • However, there is no tumor marker sufficiently specific and sensitive for healthy population screening. (biochemia-medica.com)
  • After extensive washing, the direct amount of radioactive antibody bound is measured and the amount of target molecule quantified by comparing it to a reference amount assayed at the same time. (wikipedia.org)
  • An assay in which reference ranges have been stratified in such a manner is required. (blogspot.com)