Selective bacterial isolation microbial viability. Medical search. Frequent questions

Nucleic acid amplification diagnostics offer a rapid and accurate method of diagnosing microbial pathogens. (medscape.com)
It has been used for the diagnosis of multiple viral and bacterial pathogens, including H influenzae . (medscape.com)
They represent the first line of defense against pathogens in the lower airspace and recognize microbial ligands via pattern recognition receptors ( 4 , 5 ). (frontiersin.org)
In other bacterial pathogens, genes important for pathogenicity and host range include secreted protein effectors that suppress host immunity, alter host metabolism, and enable colonization, providing a fitness advantage. (biorxiv.org)
As antibiotic resistance in microbial pathogens embodies a global threat to public health, it demands the development of novel strategies for managing microbial infections. (nature.com)
Over the last decade, research on antimicrobials has shifted towards an alternative approach to combat pathogens using anti-infective drugs that selectively interrupt virulence pathways to help prevent or cure bacterial infections. (nature.com)
Anti-infective drugs that do not perturb survival or viability of bacterial pathogens should be less likely to promote resistance than conventional antibiotics 1 , 2 . (nature.com)
Natural bioactive compounds derived from plant species show promising therapeutic properties to combat the emerging resistance in microbial pathogens, which can be exploited as next generation anti-infective drugs. (nature.com)

They support the growth of a wide range of microorganisms and are often used for routine laboratory procedures, isolation, and enumeration of bacteria. (timesofpharma.com)
Differential media contain specific additives that cause certain bacteria to display visible differences in colony appearance or color changes, allowing for the differentiation of bacterial species based on their metabolic capabilities. (timesofpharma.com)
MacConkey agar is a selective and differential medium used to isolate and differentiate Gram-negative bacteria, especially members of the family Enterobacteriaceae. (timesofpharma.com)
The medium contains a high concentration of salt, making it selective for halophilic (salt-tolerant) bacteria. (timesofpharma.com)
The microbial entities occurring in NWCs were identified by partial 16S rDNA sequencing of DGGE bands: four lactic acid bacteria (LAB) closest relative of Streptococcus thermophilus, Lactococcus lactis, Lactobacillus delbrueckii and Lactobacillus crispatus were revealed by the analysis of DNA directly extracted from NWC while two other LAB, Lactobacillus fermentum and Enterococcus faecalis, were identified by analyzing DNA from the cultivable community. (who.int)
The isolation of Yersinia enterocolitica from food is challenging, because the bacteria are readily overgrown by the concomitant microflora. (bvsalud.org)
In addition, plating of the bacteria after selective enrichment for two days and a treatment with potassium hydroxide is mandatory. (bvsalud.org)
It is not simple to keep a high number of viable probiotic bacteria in fermented milk throughout the shelf life, because their viability in the product matrix is influenced by numerous factors. (encyclopedia.pub)
Here, we review the literature on culture-independent analysis of microbial diversity in the BE and explore what it tells us about the dynamics and distribution of bacteria and fungi in these environments. (biomedcentral.com)
Reduced microbial diversity and altered function in the GI tract as a result of antibiotic exposure may contribute to the proliferation of pathogenic bacteria leading to enteric infections such as Clostridioides (previously Clostridium) difficile infection (CDI) and recurrence of CDI (rCDI) 1 , 8 . (dunianusantara.io)
The role of bacterial exopolysacchairdes in the maintenance of healthy human gut bacteria. (nld-dtp.org.uk)
After biomechanical preparation of root canals, the bacterial load is reduced by at least 95% and the remaining viable bacteria may be the cause of refractory lesions 8 . (bvsalud.org)
Often starting as a phagosome in the endocytic compartment, these bacteria-occupied vacuoles transition into organelles with novel molecular features via remodeling of host proteins and lipids through the highly coordinated actions of bacterial factors [3] [5] . (microbialcell.com)

In this study, we developed a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by combining (i) nontoxic fluorescence staining of polyphosphate granules in viable microbial cells and (ii) fluorescence-activated cell sorting (FACS) for the rapid detection and collection of target cells. (frontiersin.org)
Probiotic effects are dependent on the number of viable microbial cells that reach the human gut [ 2 ] . (encyclopedia.pub)

The developed PCR-DGGE analysis of the "cultivable" community showed good potential in evaluating microbial diversity of a dairy environment: it usefully highlighted the bias introduced by selective amplification when compared to the analysis of the total community from NWC and allowed suitability of media and growth conditions to be evaluated. (who.int)

In contrast, selectively isolating microorganisms that accumulate desirable intracellular compounds, such as high-valued organic chemicals or environmental pollutants (such as heavy metals), can be challenging, as these organisms may not grow rapidly or may not grow in a unique environment that can be utilized as a selection parameter or selective pressure. (frontiersin.org)
However, the isolation of microorganisms that accumulate valuable compounds is often a result of laborious individual strain screening, hypothesis-driven isolation methods, or by chance discovery. (frontiersin.org)
Selective media encourage the growth of specific types of microorganisms while inhibiting the growth of others. (timesofpharma.com)
Minimal media have the essential nutrients required for bacterial growth, but they lack some components, forcing microorganisms to synthesize essential molecules. (timesofpharma.com)
Transport media are used to preserve and transport clinical specimens containing microorganisms to the laboratory without compromising their viability. (timesofpharma.com)
Moreover, it could be used to complete the culture independent study of microbial diversity to give information on concentration ratios among species occurring in a particular environment and can be proposed for rapid identification of dominant microorganisms in alternative to traditional tools. (who.int)
simulations indicate that artefactual, apparent increases in the abundances of very rare taxa may be a null expectation following the selective inactivation of dominant microorganisms in a community. (biomedcentral.com)
Culture-based investigations of a small number of bacterial taxa have indicated that exposure to light, and especially ultraviolet (UV) wavelengths [ 16 , 22 - 25 ], can inactivate many microorganisms and therefore potentially reduce dust microbial community viability. (biomedcentral.com)
A coherent understanding of when mortality of viable microorganisms does or does not occur in dust, and whether different light exposures influence these processes at the microbial community scale, is still lacking. (biomedcentral.com)

Follow-up analysis confirmed that these isolates accumulate polyP, indicating that DAPI can be implemented in staining living cells and FACS can effectively and rapidly screen and isolate individual cells from a complex microbial community. (frontiersin.org)
Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. (bvsalud.org)

Combining the experimental procedure for phenotyping living cells in a microbial community with the cell recovery necessary for further cultivation will allow for an efficient initial screening process. (frontiersin.org)
Total microbial community was assessed without cultivation by analyzing DNA directly extracted from the original samples of NWC. (who.int)

The genetic and metabolic diversity of microbial species provide a wealth of possibilities for biotechnological applications. (frontiersin.org)
Microbiological growth media compositions provide the necessary nutrients and conditions to support microbial growth and facilitate the identification and differentiation of various bacterial species. (timesofpharma.com)
Mannitol Salt Agar is a selective and differential medium used to identify and differentiate Staphylococcus species. (timesofpharma.com)
A variety of enrichrnent and selective culture media that enable distinction between pathogenic and non-pathogenic Listeria species have been formulated for isolation of Listeria spp. (biomedcentral.com)
In addition, thus far, no strictly selective medium for this species is available. (bvsalud.org)
Structural analysis of the metagenomes of SL1 (agricultural soil) and SL3 (mercury-contaminated agricultural soil) using Illumina shotgun next-generation sequencing revealed the loss due to mercury contamination of 54.75 % of the microbial community consisting of an archaeal domain, 11 phyla, 12 classes, 24 orders, 36 families, 59 genera, and 86 species. (springeropen.com)

In the past few decades, many selective/differential media have been developed for accurate enumeration of Lactobacillus spp. (encyclopedia.pub)
Enumeration of Bifidobacterial Populations with Selective Media to Determine the Source of Waterborne Fecal Pollution. (edu.pl)

Bifidobacterial Diversity and the Development of New Microbial Source Tracking Indicators. (edu.pl)
The most commonly used molecular approach for exploring microbial diversity is based on the PCR amplification and sequencing of genes encoding small subunit ribosomal RNA (16S rRNA) directly from environmental sources [ 16 ]. (biomedcentral.com)
This approach readily determines several orders of magnitude more microbial diversity than culture-based methods, often discovering novel organisms with no known growth conditions, and has dramatically altered our understanding of global microbial diversity [ 14 ]. (biomedcentral.com)

One such factor is the use of antibiotic therapy to treat routine or suspected bacterial infections 1 . (dunianusantara.io)
Microbial infections are diseases that can affect your skin, lungs, brain, blood and other parts of your body. (stemcelldaily.com)
Common bacterial diseases include UTIs, food poisoning, STIs and some skin, sinus and ear infections. (stemcelldaily.com)
Antibiotics for bacterial infections. (stemcelldaily.com)
Compounds having both anticancer and antimicrobial activity have promising therapeutic potential due to their selective cytotoxicity and their potential to reduce the occurrence of bacterial and fungal infections in immune-compromised cancer patients. (biomedcentral.com)
In cases of primary endodontic infections, there is a higher bacterial load than in secondary infections 6-7 . (bvsalud.org)
It is the most important type of antibacterial agent for fighting bacterial infections , and antibiotic medications are widely used in the treatment and prevention of such infections. (wikipedia.org)
Antibiotics are used to treat or prevent bacterial infections, [29] and sometimes protozoan infections . (wikipedia.org)

In order to avoid laborious screening of single strains during targeted isolation endeavors, establishing a method to initially enrich for strains showing promising characteristics directly from a mixed population would vastly accelerate the downstream screening process. (frontiersin.org)
A selection of 36 commercial probiotic fermented dairy products from UK and Europe markets were evaluated for the numbers, types, and viability of Lactobacillus strains against the stated information on their packages. (encyclopedia.pub)
The extracts further revealed a broad-spectrum antibacterial activity against bacterial strains used in the study. (biomedcentral.com)
To test the first possibility, we measured the effect of UA on bacterial metabolism by measuring the growth of two Escherichia coli strains, OP50 and HT115, after treatment with UA. (lsd1receptor.com)

The polyP positive cells were recovered by FACS, purified by colony isolation and phylogenetically identified by 16S rRNA gene sequencing. (frontiersin.org)

The main types are culture media minimal media selective media differential media transport media indicator media Culture media contain all the elements that most bacteria need for growth and are not selective, so they are used for the general cultivation and maintenance of bacteria kept in laboratory culture collections. (wikipedia.org)

In this study, we propose a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by coupling (i) specific staining of an intracellular accumulate without compromising cell viability, and (ii) rapid detection and sorting of target cells by using fluorescence-activated cell sorting (FACS), for which wastewater sludge samples were utilized. (frontiersin.org)
Here we investigated the link between cellular cholesterol and L. pneumophila intracellular replication and discovered that disruption of cholesterol biosynthesis or cholesterol trafficking lowered bacterial replication in infected cells. (microbialcell.com)

The viability of H influenzae is lost rapidly, so clinical specimens should be inoculated to an appropriate culture media without delay. (medscape.com)
For bacterial culture, specimens were cultured in parallel on 2 media: the non- selective classic Columbia agar and the selective modified Columbia urea agar (MCUA). (who.int)

During retreatment, a microbial sample was obtained from the root canal and inoculated in a selective Enterococcus medium. (bvsalud.org)

It contains sheep blood, providing additional nutrients for bacterial growth. (timesofpharma.com)
Moreover, E. faecalis has the ability to maintain its viability for twelve months without additional nutrients. (bvsalud.org)

The long-term effectiveness of most antibiotic treatments is restricted by both pathogen drug resistance and non-target effects on the host's commensal microbial community. (nature.com)

Nonetheless, PLA and MAP (70 %N2/30 %CO2) showed an excellent synergistic antibacterial ability against spoilage indicators(total glucose, pH, TVB-N, and TBARS), bacterial counts than AP, without impairing organoleptic acceptability. (bvsalud.org)
Microbial water quality indicators are used in a variety of ways within public health risk assessment frameworks, including assessment of potential hazard, exposure assessment, contaminant source identification, and evaluating effectiveness of risk reduction actions. (nationalacademies.org)
Historic definitions of microbial indicators, such as coliforms, have been tied to the methods used to measure them. (nationalacademies.org)

Growth kinetics, scanning electron microscopy (SEM), zeta potential, and cell viability investigations showed that PLA treatment exhibited reduced bacterial growth, aided morphological alterations, and leakage in cell membrane integrity in vitro. (bvsalud.org)
Morphological changes of the bacterial cells were visualized by transmission electron microscopy (TEM) and scanning transmission electron microscopy with energy-dispersive X-ray spectroscopy (SEM EDS) analysis. (bvsalud.org)

It has been shown that Ag3PO4-based composites are highly effective agents that can eradicate 100% of bacterial populations during the 60 min photocatalytic inactivation. (bvsalud.org)

Currently, highly effective isolation methods are largely based on selection by growth capabilities. (frontiersin.org)
However, methods to analyze accumulation of specific compounds in individual living cells from a community, followed by recovery and isolation, are still limited. (frontiersin.org)
Integrated Analysis of Established and Novel Microbial and Chemical Methods for Microbial Source Tracking. (edu.pl)
2 methods: histopathological examination tion is focal, negative biopsy results do not and bacterial culture growth. (who.int)

Legionella pneumophila is an accidental human bacterial pathogen that infects and replicates within alveolar macrophages causing a severe atypical pneumonia known as Legionnaires' disease. (microbialcell.com)

There is little information available regarding the bacterial community structure in commercial zebrafish housing systems, or the time-point at which the system or biofilter reaches a microbiological equilibrium in RAS in general. (biomedcentral.com)

The gold standard for cultural detection of Y. enterocolitica in food is described in the EN ISO 10273:2017. (bvsalud.org)

This article is about treatment of bacterial infection. (wikipedia.org)

Toll-like receptors (TLRs) enable innate immune cells, such as macrophages, to recognize a wide variety of microbial ligands, thereby promoting inflammation. (frontiersin.org)
Conversely, bacterial growth within cholesterol-leaden macrophages was enhanced. (microbialcell.com)

Microbial contamination is a crucial problem that is difficult to solve for the meat industry. (bvsalud.org)
The potential to mitigate microbial cross-contamination was also investigated during SWOSS treatment. (bvsalud.org)
The purpose of this study is to use shotgun next-generation sequencing to unravel the microbial community structure of an agricultural soil, decipher the effects of mercury contamination on the structure of the microbial community and the soil physicochemistry and heavy metals content. (springeropen.com)
This study revealed that mercury contamination of the agricultural soil imposed selective pressure on the members of the microbial community, which negatively impact on their population, alter soil physicochemistry, and enriched sizable numbers of members of the community that are well adapted to mercury stress. (springeropen.com)

Specifically, we placed household dust in replicate model "rooms" with windows that transmitted visible, ultraviolet, or no light and measured taxonomic compositions, absolute abundances, and viabilities of the resulting bacterial communities. (biomedcentral.com)

Optimal growth requires the use of chocolate agar and BVCCA, which is a selective media of chocolate agar that contains bacitracin, vancomycin, and clindamycin. (medscape.com)
I will use growth studies, microbial community profiling, recombinant protein technology and biochemical analysis of degradation products and enzyme activity to elucidate the carbohydrate mediated relationship in the complex environment that is the human gut. (nld-dtp.org.uk)
Fasting can be induced if a compound affects bacterial growth, thereby reducing the amount of food available or modifying key nutrient amounts14, or acts as repellent for the worms and prevents food intake. (lsd1receptor.com)

It also reveals members of microbial community hitherto not reported to be important in mercury detoxification process. (springeropen.com)

In Chile, there are yet no studies about the presence of this bacterial genus in CAP. (bvsalud.org)

Such bacterial presence may lead to clinical symptoms of persistent chronic apical periodontitis (CAP), which has high prevalence in some countries, ranging from 40% to 61% 3-4 . (bvsalud.org)

We first examined whether GML treatment affected T cell viability. (nature.com)
The strong affinity to the bacterial cell wall, as well as the well-known biocidal properties of silver itself, increase undoubtedly the antimicrobial potential of the Ag3PO4-based composites. (bvsalud.org)

0.05) in microbial reduction between 0 and 5 ppm ozone concentration across all treatment durations for each produce type. (bvsalud.org)

ABSTRACT The study aimed to develop a rapid and simple method for the primary isolation and de- tection of Helicobacter pylori from dyspeptic patients. (who.int)

Our data reveal that the widely used anti-microbial agent GML also alters the lipid dynamics of human T cells, leading to their defective signaling and function. (nature.com)

A polyphasic PCR-DGGE approach was used to describe the microbial population occurring in natural whey cultures (NWCs) for water-buffalo Mozzarella cheese production. (who.int)

His research interest is in drug discovery involving extraction, isolation, characterization and bioactivity study of natural products from plants. (iium.edu.my)

Bacterial pneumonia and meningitis are vaccine-preventable diseases. (bvsalud.org)

These media often contain unique ingredients that reveal specific bacterial characteristics. (timesofpharma.com)

Concernant la culture bactérienne, les échantillons ont été mis en culture en parallèle dans deux milieux : la gélose Columbia classique non sélective et la gélose Columbia sélective modifiée à l'urée. (who.int)

Carolina Duarte participated in all the stages of the un hospital pediátrico de tercer nivel en Colombia - 2016 study. (bvsalud.org)

Microbial analyses of foods and biological samples are traditionally carried out by direct plating on agar media. (biomedcentral.com)

Our experimental and simulation-based results indicate that dust contains living bacterial taxa that can be inactivated following changes in local abiotic conditions and suggest that the bactericidal potential of ordinary window-filtered sunlight may be similar to ultraviolet wavelengths across dosages that are relevant to real buildings. (biomedcentral.com)
This is further supported by the plants' anti-proliferative potential, bacteriostatic, bactericidal properties and also their ability to block bacterial efflux pump systems. (biomedcentral.com)

BWOSS resulted in mean microbial reductions of up to 1.56 log CFU/mL depending on the treatment time and produce type. (bvsalud.org)

Freeze-tempered produce treated with SWOSS had a higher bacterial log reduction at 5 ppm ozone compared to 0 ppm ozone (P = 0.0013) whereas room temperature produce treated with SWOSS did not show any significant difference in microbial reduction between ozone concentrations. (bvsalud.org)
MCUA showed a higher isolation rate than classic Columbia agar (67.6% versus 44.1% of patients), and the results were obtained faster (24 hours versus 5-7 days) with more clear-cut identification. (who.int)

The paper focuses on the long-term viability of forests in this region. (who.int)

Anatomy4

Organisms6

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes32

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care3

Pathogens8

Bacteria13

Viable microbial cells2

Evaluating microbial diversity1

Microorganisms9

Isolates2

Cultivation2

Species6

Enumeration2

Diversity3

Infections8

Strains4

Colony isolation1

Differential media1

Intracellular2

Specimens2

Enterococcus1

Additional nutrients2

Antibiotic1

Indicators3

Morphological2

Inactivation1

Methods4

Legionnaires1

Microbiological1

Detection1

Infection1

Macrophages2

Contamination4

Compositions1

Growth3

Community1

Genus1

Persistent1

Cell2

Concentration1

Abstract1

Widely1

Cultures1

Extraction1

Diseases1

Reveal1

Culture1

Study1

Media1

Potential2

Treatment1

Higher2

Long-term1