AdhesionGlycoprotein ligandLigand for L-selectinSoluble E-selectinLigands for selectinsMember of the selectin familySialylVascularEnzyme-LinkeNeutrophilsGlycoproteinsRecombinantKnown as P-selectinAntibodyFamily of selectinsLectin-like domainPlatelets and endothelial cellsMediatesCD62PCD62ECluster of differentiatCD62EndotheliumAtherosclerosisAntigenExpressionSerumMoleculesBindsMetastasisBiomarkersIntegrinsELISADeficiencyPSGLMarkersTumor progressionMechanismAssayCytokinesHigh affinityInflammationHumanInflammatoryGeneTissue
Adhesion27
- The selectins (cluster of differentiation 62 or CD62) are a family of cell adhesion molecules (or CAMs). (wikipedia.org)
- Selectins bind to sugar moieties and so are considered to be a type of lectin, cell adhesion proteins that bind sugar polymers. (wikipedia.org)
- The low-affinity nature of selectins is what allows the characteristic "rolling" action attributed to leukocytes during the leukocyte adhesion cascade. (wikipedia.org)
- Selectins are part of the broader family of cell adhesion molecules . (wikidoc.org)
- However, inflammation causes the expression of cell adhesion molecules (CAM) such as P-selectin on the surface of the blood vessel wall. (wikidoc.org)
- 1997). "Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin" . (wikidoc.org)
- E-selectin (SELE) is a part of a family of divalent cation-dependent carbohydrate-binding glycoproteins or adhesion molecules. (prospecbio.com)
- We demonstrated for the first time that gene silencing of endothelial E-selectin using siRNA transfection in human umbilical vein endothelial cells (HUVECs) causes inhibition of EPC adhesion under flow conditions. (scirp.org)
- Our experiments have shown the importance of E-selectin in EPC adhesion to HUVECs and the potential utility of gene silencing of E-selectin in EPC recruitment. (scirp.org)
- Yoshida, M. and Gimbrone, M.A. Jr. (1997) Novel roles for E-selectin in endothelial-leukocyte adhesion. (scirp.org)
- The vascular adhesion molecule, E-selectin (E-sel) is responsible for the tethering and rolling of leukocytes on perivascular endothelial BM niche cells (EC). (confex.com)
- This Mouse Endothelial Leukocyte Adhesion Molecule 1 (E-Selectin) ELISA Kit from Innovative Research is intended for quantitative detection of mouse soluble E-Selectin in cell culture supernates, serum and plasma (heparin, citrate). (innov-research.com)
- Expression system for standard: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. (innov-research.com)
- Description: P selectin glycoprotein ligand 1 (PSGL-1), also called CD162 glycoprotein, functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. (molepostgazette.com)
- Methods: We tested this hypothesis prospectively using P/E-selectin adhesion molecule deficient mice that mimic the human syndrome leukocyte adhesion deficiency II. (nyu.edu)
- Stability of soluble adhesion molecules, selectins, and C-reactive protein at various temperatures: implications for epidemiological and large-scale clinical studies. (ox.ac.uk)
- BACKGROUND: We assessed the impact of sample storage conditions on soluble vascular cell adhesion molecules (sVCAM), soluble intracellular adhesion molecules (sICAM-1), soluble (s)E-selectin, C-reactive protein (CRP), and sP-selectin. (ox.ac.uk)
- The adhesion molecule L-selectin expressed on most leukocytes mediates tethering and rolling of leukocytes on activated endothelia and initiates the extravasation of leukocytes into inflamed tissues. (sfb765.de)
- Since previous studies on cellular L-selectin have indicated that glycosylation influences adhesive interactions of the adhesion molecule, we have examined whether the binding activity of L-selectin-Fcγ is affected by sialylation. (sfb765.de)
- P-selectin, a cell adhesion molecule, is an important member of the selectin family. (ijbs.com)
- Furthermore, our previous study demonstrated that P-selectin deletion inhibited melanoma metastasis by suppressing platelet adhesion and that P-selectin-mediated platelet adhesion promoted intestinal tumor growth in Apc Min/+ mice [ 1 , 2 ]. (ijbs.com)
- Systemic psoriasis treatment is correlated with E-selectin and vascular cell adhesion molecule 1 (VCAM-1) plasma concentrations and may be associated with the risk for cardiovascular disease, according to a study in Medicina . (dermatologyadvisor.com)
- Heparins exhibit blockade of P‐selectin‐mediated adhesion. (functionalfluidics.com)
- Selectins contribute to both SSRBC and neutrophil adhesion (PMN) in vitro and in SCD mouse models. (functionalfluidics.com)
- In this study, a standardized flow adhesion assay was used to evaluate the effects of sevuparin on the adhesion of sickle whole blood to cultured endothelial cells and vascular cell adhesion molecule‐1 (VCAM‐1), and sickle‐leucocyte rolling adhesion on L‐selectin. (functionalfluidics.com)
- During inflammation, binding of selectins to cell-surface glycoconjugates mediates rolling adhesion of leukocytes on vascular surfaces. (ashpublications.org)
- Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
Glycoprotein ligand7
- The best-characterized ligand for the three selectins is P-selectin glycoprotein ligand-1 (PSGL-1), which is a mucin-type glycoprotein expressed on all white blood cells. (wikipedia.org)
- P-selectin glycoprotein ligand-1 ( PSGL-1 ) is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P stands for platelet ), which is one of a family of selectins that includes E-selectin (endothelial) and L-selectin (leukocyte). (wikidoc.org)
- The mucin-like glycoprotein PSGL-1 (P-selectin Glycoprotein Ligand-1) has been cloned and sequenced. (biovendor.com)
- Leukocyte rolling in postcapillary venules of inflamed tissues is reduced in L-selectin-deficient mice and mice treated with L-selectin blocking antibodies, but the glycoprotein ligand for L-selectin in inflamed venules is unknown. (rupress.org)
- Here, we show that L-selectin-dependent rolling after P-selectin blockade is completely absent in P-selectin glycoprotein ligand-1 (PSGL-1) −/− mice or wild-type mice treated with a PSGL-1 blocking monoclonal antibody. (rupress.org)
- Description: A competitive ELISA for quantitative measurement of Human P selectin glycoprotein ligand 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (molepostgazette.com)
- Description: A competitive ELISA for quantitative measurement of Canine P selectin glycoprotein ligand 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (molepostgazette.com)
Ligand for L-selectin1
- However, PSGL-1 does not seem to be the primary ligand for L-selectin. (biovendor.com)
Soluble E-selectin1
- Erratum to: The effect of soluble E-selectin on tumor progression and metastasis [BMC Cancer. (nus.edu.sg)
Ligands for selectins2
- It was shown that glycoproteins represent the biological relevant ligands for selectins. (biovendor.com)
- It is a defect in the expression of ligands for selectins due to lack of enzymes required for expression of selectin ligands. (medscape.com)
Member of the selectin family1
- Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. (cytekbio.com)
Sialyl5
- Selectins bind to the sialyl Lewis X (SLex) determinant "NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAc. (wikipedia.org)
- This mini-review describes selectin antagonists, where the N-acetylneuraminic acid moiety of the natural ligand sialyl Lewisx is replaced by mimetics containing the essential carboxylic acid function. (chimia.ch)
- The data show that sialylation of L-selectin-Fcγ reduces binding activity to ligand epitopes containing sialyl Lewis x and sulfated tyrosine residues. (sfb765.de)
- Murine leukocytes are thought to express α2-3-sialylated and α1-3-fucosylated selectin ligands such as sialyl Lewis x (sLe x ), although monoclonal antibodies (mAbs) to sLe x or Le x reportedly do not bind to murine leukocytes. (ashpublications.org)
- This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
Vascular4
- There are three subsets of selectins: E-selectin (in endothelial cells) L-selectin (in leukocytes) P-selectin (in platelets and endothelial cells) L-selectin is the smallest of the vascular selectins, expressed on all granulocytes and monocytes and on most lymphocytes, can be found in most leukocytes. (wikipedia.org)
- E-Selectin, also known as CD62E, is expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. (rndsystems.com)
- Nishiwaki, Y., Yoshida, M., Masuda, H. and Isobe, M. (2004) Recruitment of bone marrow-derived endothelial progenitor cells to vascular endothelium involves E-selectin dependent mechanism. (scirp.org)
- The aim of this study is to evaluate serum level biomarkers of atherosclerosis lipoprotein-associated phospholipase A2 and E-selectin in patients with atherosclerotic carotid stenosis with different clinical manifestation in associated with vascular risk factors. (uran.ua)
Enzyme-Linke2
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rabbit Selectin, Endothelium (SELE) in serum, plasma and other biological fluids. (bioemm.com)
- VCAM-1 and E-selectin levels were measured with an enzyme-linked immunosorbent assay at baseline and after 12 weeks. (dermatologyadvisor.com)
Neutrophils6
- Neutrophils and eosinophils bind to E-selectin. (wikipedia.org)
- Eosinophils, like neutrophils, use sialylated, protease-resistant structures to bind to E-selectin, although the eosinophil expresses much lower levels of these structures on its surface. (wikipedia.org)
- Ligands for P-selectin on eosinophils and neutrophils are similar sialylated, protease-sensitive, endo-beta-galactosidase-resistant structures, clearly different from those reported for E-selectin, and suggest disparate roles for P-selectin and E-selectin during recruitment during inflammatory responses. (wikipedia.org)
- Lselectin is constitutively expressed on neutrophils, P-selectin is found on platelets and is stored in Weibel-Palade bodies from where it is transported to the cell surface upon endothelial activation. (biovendor.com)
- Upon stimulation of these cells, P-selectin is translocated to the plasma membrane where it functions as a receptor for monocytes and neutrophils. (rupress.org)
- We observed that P- and E-selectin bound to pronase-sensitive ligands on murine monocytic WEHI-3 cells and murine neutrophils, indicating that the ligands for both selectins are glycoproteins. (ashpublications.org)
Glycoproteins2
- All selectins are single-chain transmembrane glycoproteins that share similar properties to C-type lectins due to a related amino terminus and calcium-dependent binding. (wikipedia.org)
- Although a number of important interactions by proteins such as selectins, galectins, and sialic acid-binding immunoglobulin-like lectins are thought to mainly rely on sulfated O-glycans, our insight into the sulfotransferases that modify these glycoproteins, and in particular GalNAc-type O-glycoproteins, is limited. (lu.se)
Recombinant4
- The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. (innov-research.com)
- Recombinant L-selectin-Fcγ is widely used both as a tool to study this key step of inflammation and as an anti-inflammatory compound in animal models of inflammation. (sfb765.de)
- Different forms of recombinant human L-selectin-Fcγ were expressed in CHO and K-562 cells and were purified by affinity chromatography using Protein A-Sepharose. (sfb765.de)
- For the production of biologically active L-selectin-Fcγ conditions should be chosen that favor the generation of non-sialylated or of scarcely sialylated forms of the recombinant glycoprotein. (sfb765.de)
Known as P-selectin2
- CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. (biolegend.com)
- Recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. (lsbio.com)
Antibody5
- CD62P antibody LS-B3656 is an unconjugated mouse monoclonal antibody to human CD62P (SELP / P-Selectin). (lsbio.com)
- A monoclonal antibody from rat specific for E-Selectin has been precoated onto 96-well plates. (innov-research.com)
- Standards(NSO, W22-P557) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. (innov-research.com)
- Immunoelectron microscopy using a polyclonal anti-P-selectin antibody demonstrated immunogold localization in dense granules, morphologically indistinguishable from the ACTH granules. (rupress.org)
- Binding experiments with radiolabeled monoclonal antibody to P-selectin indicated that there was also surface expression of P-selectin on the AtT-20 cells. (rupress.org)
Family of selectins1
- The family of selectins consists of three structurally and functionally related molecules. (biovendor.com)
Lectin-like domain2
- As the leukocyte rolls along the blood vessel wall, the distal lectin-like domain of the selectin binds to certain carbohydrate groups presented on proteins (such as PSGL-1) on the leukocyte, which slows the cell and allows it to leave the blood vessel and enter the site of infection. (wikipedia.org)
- Due to a common structural element, the amino-terminal lectin-like domain, the selectins are able to bind to carbohydrate ligands. (biovendor.com)
Platelets and endothelial cells1
- P-selectin, a granule membrane protein of platelets and endothelial cells, follows the regulated secretory pathway in AtT-20 cells. (rupress.org)
Mediates3
- Each selectin has a carbohydrate recognition domain that mediates binding to specific glycans on apposing cells. (wikipedia.org)
- E-Selectin mediates the initial attachment of circulating leukocytes to the blood vessel wall during inflammation. (rndsystems.com)
- E-selectin is expressed on the surface of endothelial cells and mediates the interaction of leukocytes and platelets with endothelial cells during an inflammatory response. (prospecbio.com)
CD62P1
- Thrombin-activated human peripheral blood platelets were stained with CD62P (P-Selectin) (clone AK4) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram). (biolegend.com)
CD62E2
- E-Selectin/CD62E " has 39 results in Products. (rndsystems.com)
- Simple Plex Human E-Selectin/CD62E assay kit for use on Ella instrument. (rndsystems.com)
Cluster of differentiat1
- Selectin P ligand , also known as SELPLG or CD162 ( cluster of differentiation 162), is a human gene . (wikidoc.org)
CD621
- P-selectin (PADGEM, GMP-140, CD62) is a transmembrane protein specific to alpha granules of platelets and Weibel-Palade bodies of endotheial cells. (rupress.org)
Endothelium3
- An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. (innov-research.com)
- Description: A sandwich ELISA kit for detection of Selectin, Endothelium from Rabbit in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (bioemm.com)
- Description: A sandwich quantitative ELISA assay kit for detection of Rat Selectin, Endothelium (SELE) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (bioemm.com)
Atherosclerosis2
- Selectins are involved in constitutive lymphocyte homing, and in chronic and acute inflammation processes, including post-ischemic inflammation in muscle, kidney and heart, skin inflammation, atherosclerosis, glomerulonephritis and lupus erythematosus and cancer metastasis. (wikipedia.org)
- Researchers analyzed VCAM-1, E-selectin serum concentrations, and atherosclerosis severity in patients with plaque psoriasis and sought to determine the effects of methotrexate and adalimumab treatment for 12 weeks on the plasma levels of these molecules. (dermatologyadvisor.com)
Antigen1
- One of the reported ligands for E-selectin is the sialylated Lewis X antigen (SLex). (wikipedia.org)
Expression6
- Fluorescence immunobinding assay analysis showed that significant reduction of E-selectin surface expression in HUVECs (activated with IL-1β (10 U/mL) for 4 h) transfected with siRNA against E-selectin, but not in HUVECs transfected with LacZ siRNA (control). (scirp.org)
- The expression of P-selectin in platelets from 46 psoriasis patients, 41 healthy controls and 100 other patients with inflammatory skin disease was studied. (accessdermatology.com)
- In contrast, the surface expression of P-selectin transfected into CHO cells, which do not have a regulated pathway of secretion, did not change with 8-Br-cAMP treatment. (rupress.org)
- Furthermore, we showed that P-selectin deficiency significantly decreased tissue stiffness by inhibiting lysyl oxidase (LOX) expression. (ijbs.com)
- These results indicate that P-selectin deletion significantly decreases tumor stiffness in Rip1-Tag2 mice by inhibiting LOX expression. (ijbs.com)
- Further study demonstrated that P-selectin-mediated platelet accumulation increases tissue stiffness mainly by increasing LOX expression and thus promotes tumor growth. (ijbs.com)
Serum3
- At baseline, the serum levels of VCAM-1 and E-selectin were significantly higher in patients with an estimated SCORE risk of 10% or higher compared with patients with a risk of less than 1% ( P = .02 and P = .012, respectively). (dermatologyadvisor.com)
- No association the serum level of lipoprotein-associated phospholipase A2 and E-selectin with common stroke risk factor such as hypercholesterinemia, smoking and body mass index were found, but positive correlation of lipoprotein-associated phospholipase A2 with E-selectin was significant (p=0.00085). (uran.ua)
- ABSTRACT This study assessed changes in serum levels of cytokines IFN and IL-10 (biomarkers of in ammatory changes) and soluble biomarkers sICAM-1 and sE-selectin (biomarkers of endothe- lial dysfunction) in diabetic patients with and without nephropathy. (who.int)
Molecules1
- These parts of the selectin molecules are responsible for their targeting to different compartments: P-selectin to secretory granules, E-selectin to the plasma membrane, and L-selectin to the tips of microfolds on leukocytes. (wikipedia.org)
Binds3
- Analysis of this homology has revealed that the lectin domain, which binds sugars, is most conserved, suggesting that the three selectins bind similar sugar structures. (wikipedia.org)
- PSGL-1 can bind to all three members of the family but binds best (with the highest affinity) to P-selectin. (wikidoc.org)
- L-selectin, expressed on leukocytes, binds to ligands on some endothelial cells and on other leukocytes. (ashpublications.org)
Metastasis1
- Many studies have reported that P-selectin has significant effects on the growth and metastasis of many cancers including melanoma, lung cancer, and colorectal carcinoma. (ijbs.com)
Biomarkers1
- However, the biomarkers sICAM-1 and sE-selectin (as mechanism of this immune dysregulation markers of endothelial dysfunction) in dia- is still unclear. (who.int)
Integrins1
- CAMs have been classified into: cadherins, the immunoglobulin superfamily, integrins and selectins. (bvsalud.org)
ELISA2
- This mouse E-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. (innov-research.com)
- Lipoprotein-associated phospholipase A2 and E-selectin was measured using commercially available (ELISA) kit. (uran.ua)
Deficiency1
- Importantly, we found that P-selectin deficiency inhibited insulinoma progression using Rip1-Tag2 transgenic mice and Rip1-Tag2;P-sel -/- mice [ 3 ]. (ijbs.com)
PSGL4
- SELPLG codes for PSGL-1, the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. (wikidoc.org)
- The first step in this interaction process is carried out by PSGL-1 interacting with P-selectin and/or E-selectin on endothelial cells and adherent platelets. (wikidoc.org)
- This soluble isoform of PSGL-1 is still capable of binding to P-selectin, thus representing a competitor for cellular PSGL-1 through which regulation in many physiological and pathological processes can take place. (biovendor.com)
- To investigate whether leukocyte-expressed PSGL-1 is mediating L-selectin-dependent rolling, we reconstituted lethally irradiated wild-type mice with PSGL-1 −/− bone marrow cells. (rupress.org)
Markers1
- The values of hsCRP were higher (p = 0.002) in the T1DM-ED group (0.36 ± 0.2 mg/L) relative to control (0.15 ± 0.1 mg/L) and T1DM (0.19 ± 0.2 mg/L). The results suggest that E-selectin and hsCRP can be useful markers of ED in children with T1DM. (unl.pt)
Tumor progression1
- To explore the role of P-selectin in tissue stiffness, we demonstrated that tumor progression in Rip1-Tag2 mice was correlated with tissue stiffness using immunofluorescence and histological staining. (ijbs.com)
Mechanism2
- To investigate whether the mechanism of targeting of P-selectin to granules is specific for megakaryocytes and endothelial cells and/or dependent on von Willebrand factor, a soluble adhesive protein that is stored in the same granules, we have expressed the cDNA for P-selectin in AtT-20 cells. (rupress.org)
- We also found that large numbers of platelets accumulated in Rip1-Tag2 mouse insulinomas via a P-selectin-dependent mechanism [ 3 ]. (ijbs.com)
Assay1
- sP-selectin is not stable and therefore requires immediate assay. (ox.ac.uk)
Cytokines2
- E-selectin is not expressed under baseline conditions, except in skin microvessels, but is rapidly induced by inflammatory cytokines. (wikipedia.org)
- Cytokines such as TNF-alpha stimulate transcription and translation of E-selectin and additional P-selectin, which account for the delay of several hours. (wikipedia.org)
High affinity1
- Different putative ligand structures have been identified for which the selectins show high affinity. (biovendor.com)
Inflammation1
- As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. (molepostgazette.com)
Human2
- E-selectin is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA. (prospecbio.com)
- Therefore, P-selectin may be an effective therapeutic targeting for treating human insulinomas. (ijbs.com)
Inflammatory3
- During an inflammatory response, P-selectin is expressed on endothelial cells first, followed by E-selectin later. (wikipedia.org)
- Selectins play a key role in leukocyte trafficking during the inflammatory response of the organism, i.e. the recruitment and extravasation of leukocytes from the blood stream into inflamed tissue. (chimia.ch)
- Antagonizing the interaction of selectins with their physiological ligands was shown to be a validated approach for the treatment of inflammatory disorders like rheumatoid arthritis, stroke or reperfusion injuries. (chimia.ch)
Gene2
- Association of E-Selectin gene rs5361 polymorphism with ischemic stroke susceptibility: a systematic review and Meta-analysis. (uran.ua)
- Pleiotropy of ABO gene: correlation of rs644234 with E-selectin and lipid levels. (cdc.gov)
Tissue1
- E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). (innov-research.com)