Effector proteinsPeriplasmEnterohemorrhagicEnterotoxigenicHeterologousBacterialGenesO157:H7SalmonellaOperonAbstractPathwayStrainsBacteriaEnteropathogenicPathogenicIntracellular expressionPeriplasmicExtracellularEHECRecombinant humanBacteriumUptakeProduction of recombinant proteinPurificationEffectorsInducesGeneBiologically activeFlagellarExpressionKlebsiellaMechanismInsightsInner-membrane proteinCyclicIntestinalShigellaCytoplasmicTypeToxinGeneticsMembranesMammalian cellOuter membrane proteinBasalAntibiotic resistanceInfectionImmunity proteinSystemStaphylococcalReceptor
Effector proteins6
- The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. (frontiersin.org)
- While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). (frontiersin.org)
- In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS SPI-6 and T6SS SPI-19 gene clusters in S . Dublin. (frontiersin.org)
- This model represents the C-terminal (CT) toxin domain of CdiA effector proteins. (nih.gov)
- The locus of enterocyte effacement (LEE) in EHEC encodes a type three secretion system and effector proteins, all of which are essential for bacterial adherence to host cells. (nih.gov)
- Type III secretion systems (TTSSs) are employed by pathogens to translocate host cells with effector proteins, which are crucial for virulence. (huji.ac.il)
Periplasm10
- In Escherichia coli, the colicin M protein kills E. coli after uptake into the periplasm. (mpg.de)
- PulG is the major subunit of a short, thin-filament pseudopilus, which presumably elongates and retracts in the periplasm, acting as a dynamic piston to promote pullulanase secretion. (pasteur.fr)
- Tat-based translocation of biopharmaceuticals to the periplasm has the potential to greatly ease the downstream processing by reducing the amount of contaminating proteins and nucleic acids, thereby lowering the costs and time needed for their production. (kent.ac.uk)
- Initially, a new experimental design for the export of multiple disulphide bond containing proteins to the periplasm by Tat in the presence of CyDisCo was empirically tested and developed as a starting point for future experiments. (kent.ac.uk)
- Here, we found that alternative signal peptides namely AmiC and MdoD allow highly efficient secretion of a disulphide bond-containing protein (YebF) to the periplasm of E. coli via Tat with CyDisCo. (kent.ac.uk)
- Overall, the research work presented in this thesis suggests that the Tat pathway and the CyDisCo system are attractive platforms for biotechnology and establishes highly efficient Tatdependent secretion of disulphide-bonded protein YebF to the E. coli periplasm. (kent.ac.uk)
- However, in some cases also secretion of protein from periplasm was observed. (sharif.edu)
- Use of gene fusion to study secretion of maltose-binding protein into escherichia coli periplasm. (ard-riocht.org)
- Secretion is achieved by an inner-membrane complex assembling a short pilus to push the recruited effector from the periplasm (the space between the two membranes) through the gated pore in the outer-membrane. (ukri.org)
- We also aim to determine the organisation of domains in the periplasm, and this will help us to understand how specific proteins substrates are recruited to the secretion system and how signal is transduced between periplasm and the cytoplasm across the inner membrane assembly complex. (ukri.org)
Enterohemorrhagic6
- The goal of this study was to define the role of the NleF protein in enterohemorrhagic E. coli virulence. (k-state.edu)
- However, a significant risk exists that infections caused by highly virulent E. coli isolates, such as the enterohemorrhagic E. coli O157:H7, develop into serious and potentially lifethreatening complications, such as hemolytic uremic syndrome. (eurekaselect.com)
- We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. (cdc.gov)
- Enterohemorrhagic Escherichia coli (EHEC) pathogenicity is usually linked to a Shiga toxin ( 1 , 2 ) and virulence factors, including adhesins, toxins, invasins, protein secretion systems, iron uptake systems, and several unidentified functions ( 3 , 4 ), which are unrelated to strain phylogeny. (cdc.gov)
- IMPORTANCE EPEC and the closely related enterohemorrhagic Escherichia coli (EHEC) represent a global public health problem. (huji.ac.il)
- Cytotoxin production by other bacteria (ie, Shigella dysenteriae, Vibrio parahaemolyticus, Clostridium difficile, enterohemorrhagic E coli ) results in mucosal cell destruction that leads to bloody stools with inflammatory cells. (medscape.com)
Enterotoxigenic7
- We study several virotypes of Escherichia coli that cause diarrhea and malnutrition in humans and livestock, including E. coli O157:H7, non-O157 STEC, and enterotoxigenic E. coli (ETEC). (k-state.edu)
- Such studies are needed to understand the increasingly recognized diversity of enterotoxigenic E. coli, a leading cause of pediatric and travelers' diarrhea. (eurekaselect.com)
- Ozaki H, Sato T, Kubota H, Hata Y, Katsube Y, Shimonishi Y (1991) Molecular structure of the toxic domain of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli . (springer.com)
- Hidaka Y, Kubota H, Yoshimura S, Ito H, Takeda Y, Shimonishi Y (1988) Disulfide linkages in heat-stable enterotoxin (STp) produced by a porcine strain of enterotoxigenic Escherichia coli . (springer.com)
- Yoshimura S, Ikemura H, Watanabe H, Aimoto S, Shimonishi Y, Hara S, Takeda T, Miwatani T, Takeda Y (1985) Essential structure for full enterotoxigenic activity of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli . (springer.com)
- Human pathogens such as enterotoxigenic Escherichia coli, Vibrio cholerae and Pseudomonas aeruginosa cause children's diarrhea, cholera and respiratory infections, respectively, and there are many deaths annually. (ukri.org)
- Enterotoxins, generated by some bacteria (ie, enterotoxigenic Escherichia coli, Vibrio cholera ) act directly on secretory mechanisms and produce a typical, copious watery (rice water) diarrhea. (medscape.com)
Heterologous6
- Recent advances in genetic engineering have made it possible for microorganisms to be used for expression of heterologous proteins. (biomedcentral.com)
- In this approach, heterologous proteins are expressed either as cytoplasmic or intracellularly or secreted as extracellularly [ 11 ]. (biomedcentral.com)
- Signal peptides (SPs) are one of the most important factors for suitable secretion of the recombinant heterologous proteins in Escherichia coli (E. coli). (edu.pl)
- Baradaran A, Sieo CC, Foo HL, Illias RM, Yusoff K, Rahim RA (2013) Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. (edu.pl)
- The production of heterologous proteins of interest in microbial hosts such as Escherichia coli is a cost-effective and vital tool for the biopharmaceutical and enzyme industries. (kent.ac.uk)
- However, production of heterologous proteins still faces limitations. (rug.nl)
Bacterial17
- To achieve this goal we use a combination of x-ray crystallography, molecular modelling, molecular docking and molecular biology in collaboration with medicinal chemistry to engineer drugs that specifically interact with and disable critical bacterial target proteins. (ubc.ca)
- Additionally, bacterial virulence proteins utilize many molecular mechanisms that are also conserved among viruses of importance to biosecurity. (k-state.edu)
- My research team has discovered several mechanisms by which bacterial proteins subvert the host innate immune system to promote bacterial colonization and transmission. (k-state.edu)
- Regulation of type III secretion hierarchy of translocators and effectors in attaching and effacing bacterial pathogens. (k-state.edu)
- The bacterial virulence factor NleA inhibits cellular protein secretion by disrupting mammalian COPII function. (k-state.edu)
- Bacterial effector binding to ribosomal protein S3 subverts NF-B function. (k-state.edu)
- Sigma-54 interaction domain, Bacterial regulatory protein, Propionate catabolism activator [Interproscan]. (ntu.edu.sg)
- LysR substrate binding domain, Bacterial regulatory helix-turn-helix protein [Interproscan]. (ntu.edu.sg)
- As a pathogen, E. coli are the most frequent causes of bacterial infections, including urinary tract infections, diarrheal disease, and other clinical infections such as neonatal meningitis, pneumonia and bacteremia. (eurekaselect.com)
- Bacterial toxins can be single proteins or oligomeric protein complexes that are organized with distinct AB structure-function properties. (springer.com)
- Bacterial toxins also catalyze the non-covalent modification of host protein function or can modify host cell properties through direct protein-protein interactions. (springer.com)
- Bacterial toxins are often characterized based upon the secretion mechanism that delivers the toxin out of the bacterium, termed types I-VII. (springer.com)
- The bacterial type II secretion system delivers harmful proteins which can have devastating effects on animals, humans and plants. (ukri.org)
- The bacterial type II secretion system spans the two membranes of the Gram-negative cell wall. (ukri.org)
- Small intestinal bacterial overgrowth can result from alterations in intestinal anatomy or gastrointestinal motility, or lack of gastric acid secretion. (msdmanuals.com)
- This low bacterial count is maintained by normal peristalsis, normal gastric acid secretion, mucus, secretory IgA, and an intact ileocecal valve. (msdmanuals.com)
- Penicillins inhibit bacterial cell wall synthesis by binding to penicillin-binding proteins. (medscape.com)
Genes7
- Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. (frontiersin.org)
- The beta-galactosidase activity in strains producing a PulG-LacZ hybrid protein increased substantially when the dsbA, dsbB, or all sec genes tested except secB were compromised by mutations. (pasteur.fr)
- Genetic studies have identified six genes whose products comprise the general protein secretion machinery of Escherichia coli. (princeton.edu)
- Systematic sequencing of the Escherichia coli K-12 chromosome (GenBank entry U18997) has revealed the presence of an apparently complete operon of genes (the gspC-0 operon) similar to genes coding for components of the main terminal branch of the general secretory pathway (e.g., the Klebsiella oxytoca pulC-0 pullulanase secretion operon) and to related genes required for type IV pilus biogenesis. (pasteur.fr)
- Thus, the gsp operon of E. coli K-12 includes at least two functional genes which, together with the rest of the operon, are probably not expressed under laboratory conditions. (pasteur.fr)
- The nucleotide sequences of rbsD , rbsA , and rbsC genes of Escherichia coli K 12. (microbiologyresearch.org)
- The latter include upregulation of genes encoding intracellular stress proteins (groES/EL, CtsR regulated genes). (rug.nl)
O157:H73
- The goal of this study was to define the role of E. coli O157:H7 NleH proteins in disrupting NF-kB signaling. (k-state.edu)
- Due to the appearance of the highly virulent strain of E. coli of serotype O157:H7 in the US and Canada in the 1980's, and subsequently in other Latin American countries, there is an increase need for accurate testing for this and other pathogenic E. coli strains, substantially enhancing detection of virulent strains and, therefore, facilitating identification of sporadic E. coli infections and outbreaks. (eurekaselect.com)
- In many laboratories, sorbitol-MacConkey medium is commonly used to screen for the slow sorbitol fermentation phenotype of the most common Shiga toxin-containing strain: O157:H7 ( 5 ), but this process does not address the pathogenic potential of the remaining sorbitol-positive E. coli . (cdc.gov)
Salmonella5
- Burkoldheria pseudomallei is a Gram-negative bacterium that possesses a protein secretion system similar to those found in Salmonella and Shigella. (rcsb.org)
- BipD is similar in sequence to IpaD from Shigella and SipD from Salmonella and is therefore likely to be a translocator protein in the type-III secretion system of B. pseudomallei. (rcsb.org)
- We used this assay alongside a previously described real-time population-based translocation assay, focusing mainly on enteropathogenic Escherichia coli (EPEC) and partly comparing it to Salmonella. (huji.ac.il)
- We found that the two pathogens exhibit different translocation behaviors: in EPEC, a subpopulation that formed microcolonies carried out most of the translocation activity, while Salmonella executed protein translocation as planktonic bacteria. (huji.ac.il)
- Ampicillin is a second-generation penicillin that is active against many strains of Escherichia coli, Proteus mirabilis, Salmonella, Shigella, and Haemophilus influenzae. (medscape.com)
Operon4
- For example, the last gene in the gsp operon, gspO (formerly hopD), encodes a protein which is similar to several type IV prepilin peptidases. (pasteur.fr)
- gspO also complements a mutation in the corresponding gene (pulO) of the pullulanase secretion operon when it is expressed from lacZp. (pasteur.fr)
- Another gene in the gsp operon, gspG (formerly hopG), encodes a protein similar to prePulG, a component of the pullulanase secretion pathway. (pasteur.fr)
- Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression. (umd.edu)
Abstract3
- In abstract, these information point out that PDE10A is concerned in QUIN-mediated synaptotoxicity and its inhibition elicit neuroprotection by decreasing the oxidative stress and defending synaptic proteins through up-regulation of cAMP signalling cascade. (aabioetica.org)
- Abstract: the maltose binding protein (mbp) affinity tag has been extensively used for protein purification. (ard-riocht.org)
- abstract = "Background: Bacillus subtilis is a favorable host for the production of industrially relevant proteins because of its capacity of secreting proteins into the medium to high levels, its GRAS (Generally Recognized As Safe) status, its genetic accessibility and its capacity to grow in large fermentations. (rug.nl)
Pathway8
- The recombinant hGH was then expressed in E. coli and successfully secreted to the medium through the Sec pathway. (biomedcentral.com)
- Recombinant hGH was then expressed in E. coli and successfully secreted into cell culture medium via the Sec pathway. (biomedcentral.com)
- Chen H, Kim J, Kendall DA (1996) Competition between functional signal peptides demonstrates variation in affinity for the secretion pathway. (edu.pl)
- Research and development efforts over the past few decades have majorly focused on the Sec pathway for recombinant protein secretion in E. coli. (kent.ac.uk)
- However, the Twin-arginine translocation (Tat) pathway of E. coli has recently garnered interest for the periplasmic export of folded biopharmaceuticals as it possesses a unique proofreading ability to export correctly folded proteins. (kent.ac.uk)
- This led to an interesting comparison between the two strains based on soluble protein yields and quality when exported by the Sec pathway in fermenters. (kent.ac.uk)
- Finally, in order to understand the limitations and maximize the applicability of the Tat pathway for proteins that fail to get exported efficiently with the TorA signal peptide, we focused on investigating alternative signal peptides. (kent.ac.uk)
- Insights from mutant analysis and the biochemical properties of the purified components allows the secretion pathway to be described in some detail. (princeton.edu)
Strains6
- Multidrug-resistant, chuA -positive E. coli H18 strains and R-FLIC2 (5-CTAACCCTGCAGCAGAGACA-3), are also frequently recovered from patients with urinary we obtained an internal fl iC amplicon from 105 (80.1%) tract infection. (cdc.gov)
- The emergence and evolution of pathogenic Escherichia coli strains associated with diarrheal diseases have become a topic of active investigation in recent years due to the emergence of more virulent strains and the association of new serotypes with disease. (eurekaselect.com)
- The relative contribution of recombination events in the generation of new categories of pathogenic E. coli varies among the E. coli population, and it is represented by the wide variety of mobile elements found in different diarrheal strains (e.g. pathogenicity islands, phages, transposons, pathoadaptive mutations, etc). (eurekaselect.com)
- In addition, factors underlying the emergence of enteroaggregative and atypical enteropathogenic E. coli strains associated with persistent diarrhea are unknown. (eurekaselect.com)
- Horizontal transfer of genetic elements that affect virulence of diarrheagenic E. coli strains and changes in global agricultural processes, as well as movement of humans and animals, may contribute to the complex natural history of diarrheagenic E. coli. (eurekaselect.com)
- stx 1, stx 2, eae , and hlyA (or ehx A). Variation in amplification success is likely to increase because more virulence factor variants are certain to emerge as more EHEC and Shiga toxin-producing E. coli (STEC) strains are identified. (cdc.gov)
Bacteria9
- TolC family proteins are ubiquitous among gram-negative bacteria, and the conserved apertures present a possible chemotherapeutic target in multidrug-resistant pathogens. (umsystem.edu)
- this particular model contains the C-terminal domain of CdiA (CdiA-CT) from Escherichia coli, Klebsiella pneumoniae and other bacteria. (nih.gov)
- CDI(+) bacteria also produce a CDI immunity protein (CdiI) to specifically neutralize the CdiA-CT toxins to prevent auto-inhibition. (nih.gov)
- Many bacteria kill related bacteria by secretion of bacteriocins. (mpg.de)
- These pathogens, as well as other enteric bacteria that use contact-dependent secretion systems, represent important threats to food safety, biosecurity, and animal health. (k-state.edu)
- Escherichia coli are Gram-negative bacteria found as normal commensal flora in the gastrointestinal tract. (eurekaselect.com)
- Activated lactoferrin (ALF) is a new form of a naturally occurring protein from milk that acts as a powerful deterrent to pathogenic bacteria that may be present on a meat surface. (ift.org)
- The interaction of LF with microbial surfaces-OMPs of Gram-negative bacteria in particular-has led to other antimicrobial mechanisms, such as the inhibition of microbial attachment to sub-epithelial matrix proteins and detachment of bacteria from mucosal surfaces. (ift.org)
- Long dsRNAs were produced in Escherichia coli strain HT115 (DE3), and a concentrated suspension of heat-killed dsRNA-containing bacteria in 10% sucrose was offered on cotton balls ad-libitum to adult mosquitoes. (cdc.gov)
Enteropathogenic2
- Proteomic analysis of the intestinal epithelial cell response to enteropathogenic Escherichia coli . (k-state.edu)
- Modulation of host cytoskeleton function by the enteropathogenic Escherichia coli and Citrobacter rodentium effector protein EspG. (k-state.edu)
Pathogenic3
- Alfredo G. Torres , " Pathogenic Escherichia coli in Latin America ", Bentham Science Publishers (2010). (eurekaselect.com)
- At least six different categories of pathogenic E. coli causing enteric infections have been identified and further characterized. (eurekaselect.com)
- Understanding the population structure of pathogenic E. coli is important, since it impacts the effectiveness of molecular epidemiological studies. (eurekaselect.com)
Intracellular expression2
- Escherichia coli is widely used host for the intracellular expression of many proteins. (sharif.edu)
- The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
Periplasmic8
- Enterobactin molecules destined for secretion must cross both the inner (cytoplasmic) and outer membranes and the intervening periplasmic space, believed to be a distance of at least 130Å across. (umsystem.edu)
- Furthermore, depletion of the Ffh component of the signal recognition particle (SRP) completely abolished PrePulG* processing, without affecting the Sec-dependent export of periplasmic MalE and RbsB proteins. (pasteur.fr)
- protein_coding" "AAC74156","flgA","Escherichia coli","assembly protein for flagellar basal-body periplasmic P ring [Ensembl]. (ntu.edu.sg)
- The objective of this study was to identify a panel of signal peptides (among the 90 biologically active SPs) required for the secretory production of interferon-beta 1b (IFN-beta 1b) recombinant protein into the periplasmic space of E. coli host. (edu.pl)
- Therefore, only 59 SPs could be theoretically applied to secrete IFN-beta 1b into the periplasmic space of E. coli. (edu.pl)
- 1,3-beta-glucosidase I/II (EXG1) and outer membrane protein C (OmpC) were theoretically the most potent and desirable SPs for secretion of recombinant IFN-beta 1b into the periplasmic space of E. coli. (edu.pl)
- Later, a comparative study between Sec and Tat pathways for periplasmic export of a single chain variable fragment (scFv) in E. coli BL21 and W3110 was carried out. (kent.ac.uk)
- The assembly platform (comprising proteins CEFLM) is at the centre of the secretion system and interacts with the outer membrane secretin D via the periplasmic domains of CLM and with the cytoplasmic ATPase E via the cytoplasmic domain of L. At the heart of the assembly platform is the potential rotor protein F which facilitates the assembly of the short periplasmic pilus comprising the major pilin G and initiation pilins IJK. (ukri.org)
Extracellular8
- The production of recombinant mature hormone in suitable hosts and secretion of this therapeutic protein into the extracellular space can be considered as one of the best cost-effective approaches not only to obtain the active form of the protein but also endotoxin-free preparation. (biomedcentral.com)
- Since the natural growth hormone signal peptide is of eukaryotic origin and is not detectable by any of the Escherichia coli secretory systems, including Sec and Tat, and is therefore unable to secrete hGH in the prokaryotic systems, designing a new and efficient signal peptide is essential to direct hGh to the extracellular space. (biomedcentral.com)
- The secretion of hGH into the extracellular medium was confirmed by SDS-PAGE and Western blot analysis. (biomedcentral.com)
- Since the native signal protein peptide of S. aureus protein A was not able to deliver hGH to the extracellular space, it was modified using bioinformatics tools and fused to the n-terminal region of hGh to show that the redesigned signal peptide was functional. (biomedcentral.com)
- Choi J, Lee S (2004) Secretory and extracellular production of recombinant proteins using Escherichia coli. (edu.pl)
- Improvement of both intracellular and extracellular production of recombinant protein in E. coli is an attractive goal in order to reduce production cost and increase process efficiency and economics. (sharif.edu)
- Since heat shock proteins in E. coli were reported to be helpful for protein refolding and hindering aggregation, in this work different types of single and periodic heat shocks were tested on lab scale to enhance intracellular and extracellular protein production. (sharif.edu)
- Lumen formation occurred during L4 stage through secretion likely involving a coincident cellular apical lipid enrichment and extracellular vesicle-like structures. (cdc.gov)
EHEC1
- Enterohaemorrhagic Escherichia coli (EHEC) is a life-threatening human pathogen worldwide. (nih.gov)
Recombinant human4
- Krishna Rao DV, Ramu CT, Venkateswara Rao J, Narasu ML, Bhujanga Rao AKS (2009) Cloning, High Expression and Purification of Recombinant Human Intereferon-β-1b in Escherichia coli. (edu.pl)
- The present invention relates to a chimeric protein of recombinant human granulocyte colony stimulating factor comprising of hG-CSF fused to an affinity fusion tag at its amino terminus. (allindianpatents.com)
- The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim. (allindianpatents.com)
- One way to produce recombinant human G-CSF in soluble form is to produce chimeric protein formed by expression of a hybrid gene made by splicing together two gene sequences, In this approach, ideally GCSF would be operationaffy finked to an affinity tag so as to give chimeric protein with better solubility. (allindianpatents.com)
Bacterium1
- We are supported by our collaborator who can reverse engineer the type II secretion system in the bacterium to confirm the importance of the interactions we see in our experiments in the live bacterium. (ukri.org)
Uptake1
- The maltose-binding protein (mbp) is a component of the escherichia coli maltose/maltodextrin system, which regulates the uptake and catabolism. (ard-riocht.org)
Production of recombinant protein1
- 1986, Science 232, 61-64) led to the production of recombinant protein and large scale manufacturing of the product. (allindianpatents.com)
Purification2
- However G-CSF in E. coli is expressed as inclusion bodies in the form of insoluble protein, which necessitates the additional steps for the isolation and purification of GCSF. (allindianpatents.com)
- Conversion of this insoluble material involves often slow and complex refolding methods, making protein purification difficult. (justia.com)
Effectors3
- Natural language processing approach to model the secretion signal of type III effectors. (tau.ac.il)
- Effectidor: an automated machine-learning based web server for the prediction of type-III secretion system effectors. (tau.ac.il)
- Type III secretion system effectors form robust and flexible intracellular virulence networks. (tau.ac.il)
Induces1
- Recombinant protein manufacturing (RPP) in Escherichia coli (E. coli) typically induces metabolic burden to the cells that compromises their general development and productiveness. (stevediggle.net)
Gene3
- Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. (frontiersin.org)
- Use of this method to target doublesex (a gene involved in sex differentiation) or fork head (which encodes a salivary gland transcription factor) resulted in reduced target gene expression and/or protein immunofluorescence signal, as measured by quantitative Real-Time PCR (qRT-PCR) or fluorescence confocal microscopy, respectively. (cdc.gov)
- We sought to better understand larval SG architecture, secretion, and gene expression. (cdc.gov)
Biologically active4
- The structure suggests that the biologically active form of the molecule may be a dimer formed by contacts involving the C-terminal alpha-helix, which is the most strongly conserved part of the protein. (rcsb.org)
- Though it is a glycoprotein, non-glycosylated GCSF protein expressed in E. Coli was also found to be biologically active (Wingfieldetal. (allindianpatents.com)
- Moreover since high levels of biologically active protein expression are achieved in E. coli, it is the host of preference for producing G-CSF. (allindianpatents.com)
- Cellular aspartyl proteases promote the unconventional secretion of biologically active HIV-1 matrix protein p17. (umd.edu)
Flagellar6
- protein_coding" "AAC74157","flgB","Escherichia coli","flagellar component of cell-proximal portion of basal-body rod [Ensembl]. (ntu.edu.sg)
- protein_coding" "AAC74158","flgC","Escherichia coli","flagellar component of cell-proximal portion of basal-body rod [Ensembl]. (ntu.edu.sg)
- Flagella basal body rod protein, Flagellar basal body rod FlgEFG protein C-terminal [Interproscan]. (ntu.edu.sg)
- FlgD Ig-like domain, FlgD Tudor-like domain, Flagellar hook capping protein - N-terminal region [Interproscan]. (ntu.edu.sg)
- protein_coding" "AAC74163","flgH","Escherichia coli","flagellar protein of basal-body outer-membrane L ring [Ensembl]. (ntu.edu.sg)
- Flagellar L-ring protein [Interproscan]. (ntu.edu.sg)
Expression10
- Impact of PAP on the CREB, BNDF and synaptic proteins reminiscent of SAP-97, synaptophysin, synapsin-I, and PSD-95 expression was analysed by Western blot. (aabioetica.org)
- PAP up-regulated CREB and BDNF, and synaptic protein expression. (aabioetica.org)
- HepG2 hepatoma cells, in which the expression of TMEM199 and CCDC115 was silenced, and induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells from patients with TMEM199 mutations showed markedly increased secretion of apolipoprotein B (apoB) compared with controls. (bvsalud.org)
- Baneyx F (1999) Recombinant protein expression in Escherichia coli. (edu.pl)
- Dalton AC, Barton WA (2014) Over-expression of secreted proteins from mammalian cell lines. (edu.pl)
- Expression of gspO from lacZp promotes cleavage of two known prepilin peptidase substrates in E. coli K-12: Neisseria gonorrhoeae type IV prepilin and K. oxytoca prePulG protein. (pasteur.fr)
- Expression of gspG from lacZp leads to production of a protein which (i) is recognized by PulG-specific antiserum (and by antiserum against the Pseudomonas aeruginosa PulG homolog XcpG [formerly XcpT]), (ii) is processed in cells expressing gspO, and (iii) restores secretion in cells carrying a pulG mutation. (pasteur.fr)
- Maltose binding protein (mbp) is used in recombinant protein expression as an affinity and solubility tag. (ard-riocht.org)
- The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
- Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
Klebsiella1
- The pseudopilin PulG is an essential component of the pullulanase-specific type II secretion system from Klebsiella oxytoca. (pasteur.fr)
Mechanism2
- These data establish that TolC may be a critical component of the E. coli enterobactin secretion machinery and may represent a type of siderophore export mechanism previously undescribed. (umsystem.edu)
- Gupta DD, Saha S, Chakrabarti MK (2005) Involvement of protein kinase C in the mechanism of action of Escherichia coli heat-stable enterotoxin (STa) in a human colonic carcinoma cell line, COLO-205. (springer.com)
Insights2
- Structural Insights into Inhibition of Escherichia coli Penicillin-binding Protein 1B. (ubc.ca)
- Conclusions: The results give better insights into B. subtilis responses to protein overproduction stress and provide potential targets for genetic engineering in order to further improve B. subtilis as a protein production host. (rug.nl)
Inner-membrane protein1
- Characterization of the two conformations adopted by the T3 SS inner-membrane protein PrgK. (ubc.ca)
Cyclic2
- Mutations that alter the ability of Escherichia coli cyclic AMP receptor protein to activate transcription. (microbiologyresearch.org)
- Field M, Graf LH Jr, Laird WJ, Smith PL (1978) Heat-stable enterotoxin of Escherichia coli: In vitro effects on guanylate cyclase activity, cyclic GMP concentration, and ion transport in small intestine. (springer.com)
Intestinal2
- Heat-labile enterotoxin promotes Escherichia coli adherence to intestinal epithelial cells. (k-state.edu)
- Outbreak studies indicate that most patients with an intestinal E. coli infection develop mild, uncomplicated diarrhea. (eurekaselect.com)
Shigella1
- Enterocyte invasion is the preferred method by which microbes such as Shigella and Campylobacter organisms and enteroinvasive E coli cause destruction and inflammatory diarrhea. (medscape.com)
Cytoplasmic1
- Furthermore, coupling Tatdependent protein secretion with the CyDisCo technology (cytoplasmic disulphide bond formation in E. coli), which enables disulphide bond formation in the cytoplasm of E. coli, provides a powerful platform for the production of industrially relevant and difficult-to-express proteins. (kent.ac.uk)
Type11
- an invasion protein associated with the type-III secretion system of Burkholderia pseudomallei. (rcsb.org)
- Phylogenetic distribution and evolution of Type VI secretion system in the genus Xanthomonas . (tau.ac.il)
- Type II secretion system pilotin lipoprotein (PulS_OutS) [Interproscan]. (ntu.edu.sg)
- We have proposed a hypothesis to explain key chlamydial developmental events whereby RBs are replicating strictly whilst in contact with the host cell membrane-derived inclusion via type three secretion (T3S) injectisomes. (biomedcentral.com)
- Here, we present the solid-state NMR atomic structure of the building block of the type III secretion system needle. (rcsb.org)
- The high-resolution structure reveals unambiguously the helical handedness of the assembly, determined to be right-handed for the type III secretion system needle.Additionally, the axial rise per subunit could be extracted from the tetramer structure and independently validated by mass-per-length measurements. (rcsb.org)
- It has recenly been demonstrated that the type II secretion system is very important for nosocomial pathogen survival in humans where multi-drug resistance has developed. (ukri.org)
- Moreover, impairment of the type II secretion system has been shown to allow the innate immune system to clear infection. (ukri.org)
- Plant pathogens relying on the type II secretion system such as Dickeya, Pectobacterium and Xanthomonascause are responsible for devastating crop loss in the UK and Europe. (ukri.org)
- We have made excellent progress in imaging the type II secretion system. (ukri.org)
- We have assembled the reagents and expertise needed to determine the structure of the inner membrane assembly platform of the type II secretion system. (ukri.org)
Toxin1
- Yamasaki S, Sato T, Hidaka Y, Ozaki H, Ito H, Hirayama T, Takeda Y, Sugimura T, Tai A, Shimonishi Y (1990) Structure-activity relationship of Escherichia coli heat-stable enterotoxin: Role of Ala residue at position 14 in toxin-receptor interaction. (springer.com)
Genetics2
- In this study, using a combination of the bioinformatics design and molecular genetics, the protein A signal peptide from Staphylococcus aureus was modified, redesigned and then fused to the mature hGH coding region. (biomedcentral.com)
- Bieker, KL & Silhavy, TJ 1990, ' The genetics of protein secretion in E. coli ', Trends in Genetics , vol. 6, no. (princeton.edu)
Membranes2
- Full-thickness membranes were mounted on a Transwell device, constituted by two independent chambers, 1 × 10(6) CFU/ml of Escherichia coli were added to either the amnion (AMN) or the choriodecidual (CHD) face or to both. (biomedcentral.com)
- Selective stimulation of extra-placental membranes with E. coli , results in a tissue specific secretion of HBD1, HBD2, and HBD3 mainly in the CHD, which is the first infected region during an ascending infection. (biomedcentral.com)
Mammalian cell1
- A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
Outer membrane protein1
- CdiA secretion is dependent on the outer membrane protein CdiB. (nih.gov)
Basal1
- Flagella basal body rod protein [Interproscan]. (ntu.edu.sg)
Antibiotic resistance1
- Structural and kinetic analyses of penicillin-binding protein 4 (PBP4)-mediated antibiotic resistance in Staphylococcus aureus. (ubc.ca)
Infection1
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
Immunity protein2
System6
- Binding-protein-dependent transport system inner membrane component [Interproscan]. (ntu.edu.sg)
- In axons, microtubules are coated with microtubule-associated protein tau, a natively unfolded filamentous protein in the central nervous system. (sharif.edu)
- A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)
- We have produced and imaged the entire envelope-spanning secretion system. (ukri.org)
- We anticipate capturing the secretion system in different states and to be able to model the structural transitions between states. (ukri.org)
- We are also working from the top down using the full secretion system, for instance we recently produced the initiation complex in the E. coli IHE3034 system by deleting the major pilin subunit G and introducing the substrate SslE. (ukri.org)
Staphylococcal1
- Foods high in starch and protein are believed to favour staphylococcal enterotoxin (SE) production. (who.int)
Receptor2
- BDNF, tyrosine receptor kinase B (TrkB), and cAMP response element-binding (CREB) protein ranges within the hippocampus had been decided by the Western blot technique, and likewise serum corticosterone ranges had been assessed utilizing an ELISA package . (aabioetica.org)
- Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. (watson-int.cn)