• In this study, we systematically analyzed CD19 CAR-T cells of 10 children with R/R B-ALL enrolled in the CARPALL trial via high-throughput single-cell gene expression and T cell receptor sequencing of infusion products and serial blood and bone marrow samples up to 5 years after infusion. (nature.com)
  • Blood and bone marrow samples were collected at defined timepoints for biological and computational experiments and integrative analyses revolving around primary illness, relapse illness, and subsequent disease and immune response patterns. (cdc.gov)
  • This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
  • Together with the BD FACSLyric™ cell analyzer and BD FACSuite™ software, the BD FACS™ Universal Loader option and the BD FACSDuet™ Sample Preparation System, the BD Multitest™ 6-Color TBNK reagent provides a fully automated, standardized and efficient workflow for lymphocyte enumeration to best suit your routine immunophenotyping needs. (bdbiosciences.com)
  • The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
  • The ratio of all PD-L1-expressing cells (tumor cells, lymphocytes, macrophages) to the number of all tumor cells in a sample. (accc-cancer.org)
  • Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
  • e.g., dendritic cells, monocytes and B lymphocytes) in minimally manipulated whole blood samples. (ox.ac.uk)
  • Specialized blood tests (called flow cytometry and immunophenotyping) to characterize the abnormal lymphocytes can be done on the cells in the blood. (merckmanuals.com)
  • Blood samples were collected at different time points on days 1, 7, and 14 to measure the serum levels of cytokines by Cytometric bead assay (CBA), for the immunophenotyping of monocytes subsets, and also for the cell sorting of monocytes subsets for the functional studies. (nih.gov)
  • Researchers came up with a single-cell DNA sequencing and immunophenotyping assay for identifying and characterizing AML clones that persist after treatment. (genomeweb.com)
  • Flow cytometric immunophenotyping is a highly complex and relatively new technology. (cdc.gov)
  • Objective: To compare the morphological and cyto- chemical diagnosis of peripheral blood and bone marrow sample with flow cytometric immunophenotypic diagnosis in acute leukemia's. (alliedacademies.org)
  • A hematological normal adult sample with BD Multitest™ 6 Color TBNK in a BD Trucount™ Tube was acquired on a BD FACSLyric™ Flow Cytometer. (bdbiosciences.com)
  • BD FACSDuet™ Sample Preparation System, BD FACS™ Universal Loader, BD FACSLyric™ Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite™ applications and BD FACSuite™ Clinical BD Multitest™ 6-Color Assays are in vitro diagnostic medical devices bearing a CE mark. (bdbiosciences.com)
  • Necessity for Automation ⚫Cell counts ⚫Dx of Hemoglobinopathies ⚫Immunophenotyping ⚫Dx of Leukemias & Lymphomas ⚫Coagulation Abnormalities. (slideshare.net)
  • The samples are examined under a microscope to detect any chromosome changes (cytogenetic analysis) and other changes in the cells. (lls.org)
  • The TDI-SFC could potentially be used in any application requiring the immunophenotyping of low-abundance cells, such as in monitoring measurable residual disease in acute leukemias following affinity enrichment of circulating leukemia cells from peripheral blood. (ku.edu)
  • Immunophenotyping by flow cytometry of bone marrow or peripheral blood samples can be used to help distinguish AML from acute lymphocytic leukemia (ALL) and further classify the subtype of AML. (medscape.com)
  • Grading is performed on tissue samples, not on cytology samples. (vin.com)
  • Cell-permeant DNA dyes can be used to discriminate nucleated from anucleated cells reducing the need for time-consuming and potentially damaging RBC lysis reagents, which can be important for precious or small samples. (biostatus.com)
  • AQUIOS Lysing Reagent Kit consists of two reagents (Reagent A and Reagent B) used by AQUIOS flow cytometers to prepare whole blood samples for analysis of white blood cells. (beckman.com)
  • If the disease is extranodal, a surgical biopsy sample should be obtained from the involved organ. (medscape.com)
  • Cultures of fecal samples and multiple colonic biopsies were unremarkable. (cdc.gov)
  • A lab test that uses antibodies to identify and examine specific antigens in a tissue sample under a microscope, including specific cancer biomarkers. (accc-cancer.org)
  • 1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
  • Clinical samples often need a viability dye to evaluate sample integrity on analysis. (biostatus.com)
  • The Cancer Correlative Science Unit (CCSU) profiles clinical samples obtained from patients treated with cancer immunotherapies with the goal of identifying correlates of response and driving iterative improvements in patient stratification and/or therapeutic design. (stanford.edu)
  • Our cellular stabilization products are designed to preserve samples for extended periods while easing handling concerns and allowing for room temperature storage, cold chain-free shipping and batch processing. (streck.com)
  • Fingolimod (FTY) is known to have multiple effects on the immune system and the central nervous system (CNS) in patients with multiple sclerosis (MS). In this study, we evaluated the immunological and neurobiological effects of FTY in MS. Blood and cerebrospinal fluid (CSF) samples were collected from 15 MS patients before first FTY administration and after 4 months of FTY therapy. (quanterix.com)
  • However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
  • Our scientists and technicians utilize the latest advances in cellular immunophenotyping and next-generation sequencing based approaches. (stanford.edu)
  • We studied 15 consecutive patients with high-risk or relapsed CD19 + B-ALL treated with CD19 CAR-T cell therapy on the CARPALL study ( NCT02443831 ) and in whom adequate CAR-T cells could be isolated for subsequent analyses from cryopreserved samples of blood or bone marrow. (nature.com)
  • the demonstration consisted of immunophenotyping a model cell line, in this case SUP-B15 cells representing B-cell acute lymphoblastic leukemia (B-ALL). (ku.edu)
  • Consistency in sample processing workflows, minimising sample manipulation and cell stress results in better data. (ddw-online.com)
  • Next, we developed targeted MS assays, and applied these to cell lines and primary patient samples to refine the list of candidate tumor markers. (bmj.com)
  • During the procedure, a specialist takes a sample of a lymph node. (healthline.com)
  • This procedure allows CDC to determine the distribution of CD4 cells in a random sample of HIV-positive individuals. (cdc.gov)
  • The IMMUNOPREP Reagent System is a rapid, no-wash, standardized, whole blood preparation procedure maximizing sample to sample, and laboratory to laboratory reproducibility. (beckman.com)
  • Your doctor will collect a small sample of the fluid that circulate in the central nervous system in a procedure known as lumbar puncture. (parkwayeast.com.sg)
  • Works in a fully integrated system with BD FACSDuet™ Sample Preparation System for automated sample preparation allwoing a complete automated workflow from sample to answer, reducing hands-on time and error-prone steps. (bdbiosciences.com)
  • A method of measuring certain characteristics of cells in a sample, such as unusual markers on the surface of cells or the number of healthy and cancerous cells. (accc-cancer.org)
  • Reagent A lyses red blood cells in whole blood samples sufficiently to perform monoclonal antibody panel analysis without interference from red blood cells. (beckman.com)
  • The effective analysis of biological change and response starts with the quality of the sample. (ddw-online.com)
  • The Core utilizes state-of-the-art sample preparation, data acquisition and analysis, and sorting procedures in providing service and supporting collaborative research projects. (nih.gov)
  • A test that looks for certain genes, mutations, proteins, or other biomarkers in a tumor tissue sample. (accc-cancer.org)
  • A tumor testing method that uses multiple samples and NGS technology to detect biomarkers which drive cancer growth or may predict response to therapies. (accc-cancer.org)
  • With its single-tube format, the BD Multitest™ 6-Color TBNK reagent decreases cost by reducing the number of tubes and sample processing time. (bdbiosciences.com)
  • The training includes sample preparation, staining, and handling post sort. (nih.gov)
  • Epstein-Barr virus (EBV)+ve PTLD samples from five transplant patients were established in scid mice. (lshtm.ac.uk)
  • Both tests can be done from a blood sample. (cancer.net)
  • Participants aged 18-49 years who did not refuse the HIV antibody test and had a blood sample available for CD4+ testing were considered eligible for testing. (cdc.gov)
  • The test involves taking a blood sample for a full blood count of red blood cells, white blood cells and platelets. (parkwayeast.com.sg)
  • 10). To respond to the particular requirement data obtained from laboratory analyzers and re- of the accreditation standard for medical laborato- sults transferred into LIS, and between data en- ries, the accuracy of the entire process of data tered into LIS and then into HIS. (who.int)
  • Your doctor will take a small sample of bone marrow from the hip bone for laboratory tests. (parkwayeast.com.sg)
  • Immunophenotyping and evaluation of sphingosine-1-phosphate (S1P), neurofilament light chain (NFL), S-100 and neuron-specific enolase (NSE) levels were conducted. (quanterix.com)
  • A method of preparing biological samples for processing, staining, sectioning, and examination by a pathologist. (accc-cancer.org)
  • Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC). (cdc.gov)
  • Discrepancy between any two or more test results for a single sample. (accc-cancer.org)
  • This gives the end user most flexibility with regard to application of TransFix to research samples. (nordicbiosite.com)
  • A CBC measures the number of different types of cells in a sample of a person's blood. (cancer.net)
  • The study of chromosomes, including testing samples to look for chromosomal changes such as broken, missing, or extra chromosomes. (accc-cancer.org)