Samples cell line. Medical search. Frequent questions

By adapting an existing facility used to process clinical samples for genomic assays, Broad Institute has quickly scaled the testing center to create capacity to process approximately 2,000 COVID-19 tests per day. (broadinstitute.org)
EBV-immortalized B-cell lines are an indefinite source for genomic DNA, mRNA and proteins from a selected individual. (tau.ac.il)
Implementation of a prostate cancer-specific targeted sequencing panel for credentialing of patient-derived cell lines and genomic characterization of patient samples. (broadinstitute.org)
For example, targeted sequencing provides a flexible, rapid, and cost-effective method for genomic assessment of patient-derived cell lines to evaluate fidelity to initial patient tumor samples. (broadinstitute.org)
The targeted panel also identified genomic alterations in cell-free DNA and circulating tumor cells (CTCs) from patients with metastatic prostate cancer, and in standard prostate cancer cell lines. (broadinstitute.org)
Targeted sequencing of patient-derived cell lines highlights the challenge of deriving cell lines from primary prostate cancers and the importance of genomic characterization to credential candidate cell lines. (broadinstitute.org)
Genomic DNA was extracted and purified from HCT116 cells using Qiagen QiaAMP Kit according to standard instructions. (nih.gov)
Laboratories often cannot obtain reference materials that encompass the necessary scope of variants and variant types because the supply of available characterized genomic DNA samples from cell lines or patient samples is limited and does not cover many clinically relevant variants and/or variant types. (cdc.gov)
These challenges may be solved, at least in part, by supplementing genomic DNA samples with in silico (electronic) reference materials to develop and validate the informatics pipeline used to analyze the data generated during the physical sequencing of the patient sample. (cdc.gov)

Western blotting was employed to determine NGF level in PC and paracarcinoma tissues and in PC cell lines as well as pancreatic ductal epithelial cells. (hindawi.com)
however, no prostate cancer-specific mutations were identified in the tumor-derived cell lines, suggesting preferential outgrowth of normal prostate epithelial cells. (broadinstitute.org)
What is an Epithelial Cells in Urine Test? (medlineplus.gov)
Epithelial cells are a type of cell that covers the inside and outside of the surfaces of your body. (medlineplus.gov)
An epithelial cells in urine test looks at a sample of your urine (pee) under a microscope to count the number of epithelial cells in your urine. (medlineplus.gov)
It's normal to have a small number of certain types of epithelial cells in urine. (medlineplus.gov)
An epithelial cells in urine test is a part of a urinalysis , a test that measures different substances in your urine. (medlineplus.gov)
An epithelial cells in urine test is part of a microscopic exam of urine. (medlineplus.gov)
Why do I need an epithelial cells in urine test? (medlineplus.gov)
Your provider may have ordered an epithelial cells in urine test as part of your regular checkup or if the results of your visual or chemical urine tests weren't normal. (medlineplus.gov)
Is there anything else I need to know about an epithelial cells in urine test? (medlineplus.gov)
There are three types of epithelial cells that line the urinary tract. (medlineplus.gov)
If there are squamous epithelial cells in your urine, it may mean your sample was contaminated. (medlineplus.gov)

It has been discovered that a minimal change in the resonance of the acoustic field (i.e., introducing a sample with a slightly different density) can lead to a collapse of the trapping force within the cell, limiting the viability of this method to acoustically closely matched samples and solvents. (tuwien.at)
ADH activity and ALDH expression were much lower in SNU449 compared with Huh-7 cells and at the 270 mM dose, CAE decreased cell viability by about 50% and 80%, respectively, in Huh-7 and SNU449 cells but induced mortality only in Huh-7 cells. (springer.com)
MTT, Transwell, scratch, western blotting and reverse transcription‑quantitative PCR assays were performed to examine how curcumol inhibited CRC cell viability, invasion and migration, and to detect the role of miR‑30a‑5p and curcumol in the invasion and Hippo signaling pathways of CRC cells. (spandidos-publications.com)
The results demonstrated that miR‑30a‑5p downregulation was accompanied by the inactivation of the Hippo signaling pathway, which was demonstrated to promote CRC cell viability, invasion and migration. (spandidos-publications.com)
An improved tumor necrosis factor (TNF) bioassay using an isolated subclone from the murine fibroblastoid NCTC-clone-929 cell line and a new fluorescence indicator system for detecting target cell viability was described. (cdc.gov)
Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). (bvsalud.org)

Diagnostic samples showed a typical spread of subtypes with consistent gene expression profiles across matched fresh, frozen, and formalin-fixed paraffin-embedded tissues. (aacrjournals.org)
NGF level was preeminently higher in PC tissues and cell lines than in paracarcinoma tissues and normal pancreatic epithelial cell lines. (hindawi.com)
The present study revealed that miR‑30a‑5p expression was downregulated in human CRC tissues and cells. (spandidos-publications.com)
The prostate cancer-specific panel identified biologically and clinically relevant alterations, including point mutations in driver oncogenes and ETS family fusion genes, in tumor tissues from 29 radical prostatectomy samples. (broadinstitute.org)
Transcriptome analysis has enhanced our understanding of the molecular constituents of cells and tissues. (biomedcentral.com)
But for the most part, normal tissues and 9 cell lines). (lu.se)

For researchers who wish to convert T/S ratio to base pairs (bp), the formula is (3,274 + 2,413 * (T/S)). The conversion from T/S ratio to bp is calculated based on comparison of telomeric restriction fragment (TRF) length from Southern blot analysis and T/S ratios using DNA samples from the human diploid fibroblast cell line IMR90 at different population doublings. (cdc.gov)
Although the cytotoxicity of endodontic sealers has been extensively investigated, most previous studies have used fibroblast cell lines. (bvsalud.org)

DLBCL segmentation of patient tumor samples is possible using a number of expression platforms. (aacrjournals.org)
Overall, the USF team compared results on tumor samples from a total of 148 patients. (genomeweb.com)

TAMs are the major inflammatory cells in the TME, and are closely related to poor outcomes in several types of tumors [ 11 ]. (nature.com)
In addition to these four pathways, tumors can undergo infiltration around nerve fibers and metastasis along nerves, that is, perineural invasion (PNI), which refers to the phenomenon of perineural invasion by tumor cells filling the perineurial space, wrapping around nerves in a continuous concentric sheath-like pattern, infiltration, and metastasis of extension along nerves around nerve fibers or into perineurium within the perineurium [ 7 ]. (hindawi.com)
Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
However, the successful application of these emerging cell-based therapies in solid tumors remains limited. (nature.com)
We generated patient-derived cell lines from primary prostate cancers using conditional reprogramming methods and applied targeted sequencing to evaluate the fidelity of these cell lines to the original patient tumors. (broadinstitute.org)
The panel detected driver mutations in a diverse set of clinical samples of prostate cancer, including fresh-frozen tumors, cell-free DNA, CTCs, and cell lines. (broadinstitute.org)
Dr. Renwick's research involves examining at microRNAs in tissue samples from neuroendocrine tumors. (queensu.ca)
The authors used artificial neural croarrays is to study changes in gene ex- .nature organ the tumor arose from, how big it networks (ANNs) to identify gene-ex- pression that accompany changes in is, and what it looks like after it has pression signatures associated with spe- cell physiology, such as during develop- been imbedded in wax and colored cific subtypes of tumors. (lu.se)
25 independent samples (14 tumors, 2 generally reflected in related patterns of fluorescein. (lu.se)
0 blue-cell tumors (SRBCTs), which type, 41 (including 7 expressed often masquerade as each other. (lu.se)
Although Hürthle cell carcinoma was previously considered a variant of follicular cell neoplasms, which are generally less aggressive, the 2017 World Health Organization (WHO) classification of endocrine tumors reclassified it as a distinct entity. (medscape.com)
5] The 2022 WHO classification of endocrine tumors has replaced the term Hürthle cell carcinoma with oncocytic carcinoma. (medscape.com)
Overall, only about 33% of Hürthle cell tumors demonstrate signs of that invasive growth that indicates malignancy and the possibility of metastasis. (medscape.com)
On balance, Hürthle cell tumors may be considered to be more likely to metastasize than follicular tumors. (medscape.com)
Permissive histologic interpretation may result in the designation of some non-neoplastic Hürthle cell lesions as malignant tumors. (medscape.com)

This set of Institutional Best Laboratory Practices is designed for the authentication of cell lines and tissue samples used in biomedical research. (iclac.org)
The document replaces a model policy for authentication of cell lines and tissue samples that was developed previously by the same authors for this purpose. (iclac.org)
Tanis' work uses both cell lines and patient tissue samples. (otago.ac.nz)

Next, we developed targeted MS assays, and applied these to cell lines and primary patient samples to refine the list of candidate tumor markers. (bmj.com)
Results We identified 696 MM cell surface N -glycoproteins by CSC, and developed 73 targeted MS detection assays. (bmj.com)
MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and Transwell assays were employed to test cell proliferation, invasion, and migration, respectively. (hindawi.com)
GeT-RM used targeted and whole genome sequencing analysis to recharacterize 137 DNA samples that were previously characterized using a variety of targeted genotyping assays to identify star allele diplotypes for CYP2C8, CYP2C9, and CYP2C19. (cdc.gov)
The data from each test method and consensus diplotypes for each sample, assays used, and alleles tested can be viewed here . (cdc.gov)

High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
Finally, we studied the effects of arsenide trioxide and 5-azacitidine on the proliferation of leukemia cells and the TGF- β signaling pathway. (hindawi.com)
At the same time, arsenide trioxide and 5-azacitidine are associated with the inhibition of cellular proliferation of acute leukemia cells and also promote the elevated expression of TGF- β signaling pathway-linked proteins, including TGF- β , Smad2, Smad3, and Smad4. (hindawi.com)
Introduction of SL8-specific CD8+ T cells derived from OT-1 transgenic mice resulted in a threefold increase in OT-1 T cell proliferation in HBB animals, as compared to wild-type animals. (bvsalud.org)

Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
Numerous mechanisms such as acquisition of cancer stem cells (CSCs), a subpopulation characterized by self-renewal and differentiation abilities can explain HCC aggressiveness. (springer.com)
It is involved in cell differentiation, maintenance of cell differentiation state, tumorigenesis, and cell senescence, mainly through DNA methylation, histone acetylation, and RNA-related silencing [ 6 ]. (hindawi.com)
therefore, definitive differentiation of Hürthle cell carcinoma from Hürthle-cell adenoma is based on vascular invasion and/or capsular invasion, as well as on permanent histologic sections or extrathyroidal tumor spread and lymph node and systemic metastases. (medscape.com)

Primary and metastatic prostate cancers have low mutation rates and recurrent alterations in a small set of genes, enabling targeted sequencing of prostate cancer-associated genes as an efficient approach to characterizing patient samples (compared to whole-exome and whole-genome sequencing). (broadinstitute.org)

Candidates of interest detected by MS on MM patient samples were further validated using flow cytometry (FCM). (bmj.com)
Detection of TROP‑2 in PC‑3 Human Cell Line by Flow Cytometry. (rndsystems.com)

1 2 Cell surface antigens CD38 and CD138 can be used to distinguish normal cells from clonal plasma cells, but more extensive use of immunophenotyping has been limited by a lack of universally accepted markers of MM. 3-5 Once diagnosed, the current standard-of-care for MM includes immunomodulatory drugs, proteasome inhibitors, steroids, and antibody therapies. (bmj.com)
As the complexity of the HLA system defined by antibody reagents grew, the Cell Exchange focused its efforts on providing challenging cell types - those that included "rare" HLA antigens or unusual combinations of antigens that might be difficult to discriminate. (uclahealth.org)
Western Blot: ITPR2 Antibody [NB100-2466] - 2 sec exposure using NB100-2466 on 80 ug total cell lysates listed above. (novusbio.com)
Primary BA.1 infections yielded reduced neutralizing antibody levels against WT, Delta, and Omicron BA.2, while samples from BA.2-infected individuals showed almost no cross-neutralization against the other variants. (frontiersin.org)
Here, we determined neutralizing antibody titers in serum samples collected after primary as well as vaccination-breakthrough infections with Omicron variants BA.1 or BA.2. (frontiersin.org)
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 PE-conjugated Monoclonal Antibody (Catalog # FAB650P, filled histogram) or isotype control antibody (Catalog # IC003P , open histogram). (rndsystems.com)
ScRNA-seq of PBMCs was analyzed using 20 cell surface marker antibody sequencing to annotate clusters and 399 immune response panel to identify gene expression. (bvsalud.org)
Using cluster sampling, 72 eligible infants were tested for antibody against the 3 poliovirus serotypes according to WHO guidelines. (who.int)

The objective of this study was to uncover distinct cellular and genetic signatures of transplant operational tolerance (TOT) in kidney transplant recipients (KTRs) through single cell RNA sequencing (scRNA-seq) using peripheral blood mononuclear cells (PBMCs). (bvsalud.org)

In vitro, most HRVs and HEVs differ by genotype associated with respiratory symptoms and acute their optimal growth temperature, acid tolerance, receptor otitis media, confi rming the close relationship between rhi- usage, and cell tropism. (cdc.gov)
To reveal that the NGF-TrkA signaling pathway was closely associated with PC PNI, in vitro neuroinvasion model was established by using MiaPaCa-2 cells via coculturing DRG cells in Matrigel. (hindawi.com)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
In vitro studies demonstrate that exogenous recombinant Gal3 is internalised by neuronal cell lines and primary neurons where it interacts with endogenous αSyn fibrils. (lu.se)
In line with our in vitro studies, under these conditions, genetic deletion of GAL3 leads to increased intracellular αSyn accumulation within dopaminergic neurons and remarkably preserved dopaminergic integrity and motor function. (lu.se)
In spite of the advantages of the in vitro tests, they are not able to mimic the orchestrated role of cells present inperiradicular region and the long-term cytotoxicity presented by the sealers. (bvsalud.org)
In order to take a new approach to previous in vitro studies on the cytotoxicity of endodontic materials, it would be interesting to evaluate the response of fibroblasts and macrophages, which are cell lines involved in periapical inflammation and repair. (bvsalud.org)

These are additional data for 137 DNA samples obtained during the GeT-RM PGx characterization study ( J Mol Diag 2016 Jan:18(1) 109-23 ). (cdc.gov)
These are additional data about 107 DNA samples obtained during the GeT-RM PGx characterization study ( J Mol Diag 2010 12(6):835-846 ). (cdc.gov)

Methods In this study, we first used a mass spectrometry (MS)-based discovery-driven cell surface capture (CSC) approach to map the cell surface N -glycoproteome of MM cell lines. (bmj.com)
In all the exchanges, the goals are to provide samples to validate methods and reagents, and to provide external quality control. (uclahealth.org)
GeT-RM used a variety of methods to characterize DNA from 30 Coriell cell lines to create a panel of reference materials for TMPT and NUDT15 ( J Mol Diag 2022 24:1079-1088 ). (cdc.gov)
The CYP2D6 gene in DNA from 179 cell lines including rare and/or difficult to analyze alleles and/or diplotypes were characterized by the GeT-RM using a variety of methods ( J Mol Diag 2019 21:1034-1052 ). (cdc.gov)
DNA from 137 cell lines was characterized by the GeT-RM for 28 PGx genes using a variety of methods. (cdc.gov)
DNA from 107 cell lines was characterized for CYP2D6, CYP2C19, CYP2C9, VKORC1 and UGT1A1 by the GeT-RM using a variety of methods. (cdc.gov)

Secondly, we employed qRT-PCR and Western blotting to detect the mRNA and protein levels of GRHL2 in leukemia cell lines. (hindawi.com)
Then we investigated the demethylating effect of arsenic trioxide and 5-azacitidine on the mRNA and protein expression levels of GRHL2 in cell lines of acute leukemia. (hindawi.com)
The methylation level of the GRHL2 promoter region in acute leukemia patients and cell lines was significantly higher than the normal control group, and we found the elevated mRNA and protein levels of GRHL2 in acute leukemia cell lines after the use of the demethylation drug arsenic trioxide and 5-azacitidine. (hindawi.com)
Serum samples obtained after WT infections and three-dose mRNA vaccinations with and without prior infection were included as controls. (frontiersin.org)
Furthermore, treatment with splicing inhibitor shifts the translation of the pre-mRNA to monosomal fractions and results in an increase of intron-derived peptide substrate as shown by polysome profiling and cell imaging. (bvsalud.org)
TDO mRNA was expressed in both maternal and fetal sides of the placentas and TDO protein also in the villi and it was co-expressed with IDO1 in almost half of the placental cells at these sites. (bvsalud.org)
tein, and neuronal cell adhesion molecule mRNA were also increased in olfactory bulb. (cdc.gov)

The high throughput was achieved by either a sample pooling or a cassette dosing approach, along with the use of a rapid, simple and sensitive direct injection/on-line guard cartridge extraction/tandem mass spectrometric assay that was also developed in this study. (nih.gov)
RIP assay was performed to validate NGF and miR-21 binding ability in MiaPaCa-2 cells. (hindawi.com)
Each assay plate contained 96 control wells with 8 control DNA samples. (cdc.gov)

operating procedures for the sharing of biological samples and Associated Phenotypic Data. (nih.gov)
Critical to sub-classify SRBCTs, Khan et al .6 used to measure genome-wide gene expres- .com decisions in cancer therapeutics are to microarrays to monitor the expression sion for a variety of biological samples. (lu.se)

We devised a scoring system using 145 genes from published datasets to categorize DLBCL samples. (aacrjournals.org)
Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)
This tab shows the genes with no mutations in this sample. (sanger.ac.uk)
In addition, NIA supports infrastructure and related policies to facilitate data and sample sharing arising from its Alzheimer's Disease (AD) Genetics Initiative, a research initiative to assist in the identification of the risk and protective factor genes/loci for Alzheimer's disease. (nih.gov)
Using published data from two standard RNA reference samples, we observed a strong concordance of log2 fold change for all genes when comparing AmpliSeq to Illumina HiSeq (Pearson's r = 0.92) and Ion Torrent Proton (Pearson's r = 0.92). (biomedcentral.com)
Transcript analysis of B cells revealed that genes involved in allo-immune pathway were downregulated in TOT. (bvsalud.org)

In response to endogenous and exogenous insults, malignant cells mostly have an intensive DNA repair capacity that allows them to proliferate and survive. (nature.com)
Background Multiple myeloma (MM) is characterized by clonal expansion of malignant plasma cells in the bone marrow. (bmj.com)

Many of these extracts were from the reference cells sequenced for rare alleles, thus giving laboratories the opportunity to type them, whereas they may not detect them in routine typing them in their local populations. (uclahealth.org)
The most affordable and popular service to identify line type. (searchbug.com)
This service does not check for line number portability (LNP) and will report the original phone number carrier and line type. (searchbug.com)
Since vast majority of number porting requests is from one cell phone number carrier to another, line type accuracy is reasonably high. (searchbug.com)
Line type accuracy is for Landline vs Cell Phone. (searchbug.com)
The best choice, if you need a near 100% accuracy for line type and the current phone number carrier. (searchbug.com)
This service will report new carrier and the new line type, even if the number was ported today! (searchbug.com)
This is the Line Type and Carrier (Advanced) service with a check if number is on the Federal Do Not Call (DNC) list. (searchbug.com)
You get near 100% accuracy for line type and the current carrier. (searchbug.com)
This service also shows if phone number is on the Federal Do Not Call (DNC) list and includes everything from the Advanced Line Type and Carrier (LNP) service above. (searchbug.com)
Identify Line Type in a Batch process. (searchbug.com)
Human TROP-2, also known as Tumor Associated Calcium Signal Transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. (rndsystems.com)
Hürthle cell carcinoma of the thyroid gland is an unusual and relatively rare type of differentiated thyroid cancer. (medscape.com)
The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests. (bvsalud.org)
The growth of MCA sarcoma cells expressing the intron-derived SL8 epitope was suppressed in wild-type animals compared to HBB mice. (bvsalud.org)

Samples comprise tissue biopsies and cell lines. (wikipedia.org)

Diagnosis of MM is based on morphological features, imaging studies, analysis of myeloma cell-produced proteins, and testing of the blood, urine, and bone marrow (BM). (bmj.com)

Circos plots are available only for samples sequenced using whole genome screening. (sanger.ac.uk)
The genome browser is available only for samples sequenced using whole genome screening. (sanger.ac.uk)

Next, we used methylation-specific polymerase chain reaction (MSP) technology for detecting the methylation of GRHL2 in clinical samples with acute leukemia and cell lines. (hindawi.com)

Results from biopsy samples across platforms were remarkably consistent, in contrast to published IHC data. (aacrjournals.org)
I am considering offering folks in all 50 states a kit to take air samples and they send samples back to me for testing and I get samples tested and then, I go over test results with the client. (activerain.com)
NGF promoted MiaPaCa-2 and CFPAC-1 cell invasion and migration, while Tanezumab treatment showed the opposite results. (hindawi.com)
The goal of providing unusual types or combinations to participants was often accomplished by identifying cells that gave ambiguous or incomplete results. (uclahealth.org)
The lab returned its first batch of results back to physicians within 24 hours of receiving samples. (broadinstitute.org)
This lab is authorized to process samples collected in hospitals and other clinical settings and then deliver the results securely back to physicians. (broadinstitute.org)
The error due to sampling for results based on the entire sample could be plus or minus four percentage points. (cbsnews.com)
Results are often reported as a general amount, such as "few," moderate," or "many" cells. (medlineplus.gov)

Michelle Cipicchio, process development manager, prepares a plate of samples to be run through the automated viral RNA extraction process. (broadinstitute.org)

4,6,8,10-11 The behavior of other inflammatory cells present in the periapical region has not been widely assessed, although they also contribute to the intensity of the biological response to these materials. (bvsalud.org)

One letter difference in the DNA sequence can draw the line between someone getting ill or not. (otago.ac.nz)

Curcumol treatment was identified to increase miR‑30a‑5p expression and to activate the Hippo signaling pathway, which in turn inhibited the invasion and migration of CRC cells. (spandidos-publications.com)
Overexpression of miR‑30a‑5p enhanced the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. (spandidos-publications.com)

This poll was conducted by telephone April 7-9, 2017 among a random sample of 1,006 adults nationwide. (cbsnews.com)

Microbial strains and cell lines deposit under the Budapest Treaty. (pasteur.fr)

They are called transitional cells, renal (kidney) tubular cells, and squamous cells. (medlineplus.gov)

Phone Validator: Identify Landline, Cell or VoIP Phone Numbers. (searchbug.com)
For the landline sample, a respondent was randomly selected from all adults in the household. (cbsnews.com)

The most established subtyping approach, the "Cell of Origin" (COO) algorithm, categorizes DLBCL into activated B-cell (ABC) and germinal center B-cell (GCB)-like subgroups through gene expression profiling. (aacrjournals.org)
Controversy exists about the origin of Hürthle cells, which generally are thought to derive from the follicular epithelium. (medscape.com)

Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease with distinct molecular subtypes. (aacrjournals.org)
In 1994, the Cell Exchange initiated offering the same cells for molecular typing as for serologic typing for Class I. The data from the parallel typings is instrumental in identifying serologic equivalents for Class I and Class II alleles that previously had little or no serologic information. (uclahealth.org)
The QIAseq Targeted DNA Panels have been developed as a complete Sample to Insight solution to enable digital DNA sequencing by utilizing molecular barcodes. (qiagen.com)

For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
2] Oncocytic cells in the thyroid are often called Hürthle cells, and oncocytic change is defined as cellular enlargement characterized by an abundant eosinophilic granular cytoplasm as a result of accumulation of altered mitochondria. (medscape.com)
Heatmap hierarchical clustering showed that overall cellular distribution pattern was distinct in TOT in comparison with those in the other two groups, with the proportion of B cells being higher in TOT, attributed to immature B cell fraction (TOT vs. STA vs. BPAR: 4.61% vs. 1.27% vs. 2.53%, p = 0.01). (bvsalud.org)
This study showed that TOT has distinct cellular and genetic signatures such as increases of immature B cells, naïve T cells and Tregs and high expression levels of CCR6 in Tregs. (bvsalud.org)

In vivo tumorigenesis experiments, Tanezumab markedly alleviated nerve invasion of PC cells as well as relieved nociceptive conduction in animal models. (hindawi.com)

Mechanistically, NGF could upregulate TDE-miR-21-5p levels, and DRG cells took up TDE to activate the Warburg effect and stimulate nociceptor gene expression. (hindawi.com)
In 2005, the International KIR Exchange was integrated within the framework of the International Cell Exchange, by offering reference DNA samples for KIR gene typing. (uclahealth.org)
When analyzing four closely related hiPSC-CM lines, we show that both AmpliSeq and RNA-seq capture similar global gene expression patterns consistent with known sources of variations. (biomedcentral.com)
MicroPheno: predicting environments and host phenotypes from 16S rRNA gene sequencing using a k-mer based representation of shallow sub-samples. (cdc.gov)

Early stage Huh-7 and advanced SNU449 liver cancer cell lines were subjected to chronic alcohol exposure (CAE), at different doses for 6 months followed by 1-month alcohol withdrawal period. (springer.com)
It has been reported that numerous miRNAs, including the miR-30 family, serve important roles in the regulation of cancer cell migration and invasion ( 5 - 7 ). (spandidos-publications.com)
We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)

CAE induced a dose-dependent increase in cell migration and invasion and also in the expression of cancer stem cells markers (CD133, CD44, CD90). (springer.com)
TrkA blocking-up could restrain NGF induced PC cell migration and neural invasion. (hindawi.com)
It has been reported that miR-30a-5p expression is decreased in primary gallbladder cancer (GBC) lesions, and inhibition of its expression in GBC cells promoted migration and invasion ( 8 ). (spandidos-publications.com)

Discard supernatant and resuspend cell pellet in 1 mL of PBS. (mdanderson.org)

However, the apparent permeability coefficients of the test drugs across Caco-2 monolayers measured by the sample pooling or cassette dosing (up to five drugs co-administered in this study) strategy were in good conformity with the data obtained by single-drug dosing followed by discrete sample analysis. (nih.gov)
It exists in two versions, biomedical and plant, depending on the species of the underlying microarray and RNAseq as well as single-cell RNA-sequencing data. (wikipedia.org)
All data are manually curated, quality-controlled and enriched for sample and experiment descriptions derived from corresponding scientific publications. (wikipedia.org)
By offering a forum for data exchange and discussion, the Cell Exchange has played a key role in international standardization. (uclahealth.org)
This tab shows an overview of the data that we have for this sample. (sanger.ac.uk)
There is no mutation spectrum data for this sample. (sanger.ac.uk)
NCRAD is a national repository established to facilitate access by qualified investigators to samples from well phenotyped cases and controls, and longitudinally followed, multiply affected families along with Associated Phenotypic Data for the study of the genomics of late onset Alzheimer's disease. (nih.gov)
The data reported so far yielded a heterogeneous picture with respect to the degree of cross-neutralization between Omicron and pre-Omicron variants, with most studies using samples from pre-immune, i.e. vaccinated and/or convalescent, individuals ( 10 - 17 ). (frontiersin.org)
The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. (rndsystems.com)
Samples and clinical data from patients and healthy volunteers. (pasteur.fr)
The data from each method and consensus diplotypes for each sample can be viewed here . (cdc.gov)
If you need help reading any of the data tables in the PDFs and Excel spreadsheets on this page, please use the phrase "GeT-RM Data Tables: Assistance with reading" in the subject line of your email. (cdc.gov)
Most of the findings from the analyses of the blood samples are available immediately such as the data on cholesterol and triglyceride levels in the population. (cdc.gov)
The samples were assayed on duplicate wells, resulting in 6 data points. (cdc.gov)

Our study supports that a prostate cancer-specific targeted sequencing panel provides an efficient, clinically feasible approach to identify genetic alterations across a spectrum of prostate cancer samples and cell lines. (broadinstitute.org)

The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)
CAE in Huh-7 cells selectively activated ERK1/2 and inhibited GSK3β signaling pathways. (springer.com)

The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)

The folded capillary cell provides the benefits of the accuracy of measurement of a capillary cell with the ease of use of a disposable cell. (dksh.com)
And this is the equipment that you will need-- PCV centrifuge, microhematocrit reader, clay sealant, micro-centrifuge capillary tubes, glass waste container, selection of gloves, and, of course, your blood sample-- in this case, collected in potassium EDTA tube kept in the fridge until before this demonstration. (cabi.org)

In the present study, we demonstrate that B7-H3 CAR-T cells targeting the IgC domain are more active than those targeting the IgV domain in pancreatic ductal adenocarcinoma (PDAC) and neuroblastoma (NB) mouse preclinical models. (nature.com)
This is to demonstrate how to measure packed cell volume. (cabi.org)

Prevalence and genetic analysis of mcr-3 -positive Aeromonas species from humans, retail meat, and environmental water samples. (cdc.gov)

Macrophages are the prevalent cells in inflammatory infiltrates that respond to sealers 12-13 . (bvsalud.org)

MS-based validation using primary specimens detected 30 proteins with significantly higher abundance in patient MM cells than controls. (bmj.com)

Nine of these proteins were identified as potential immunotherapeutic targets, including five that were validated by FCM, confirming their expression on the cell surface of primary MM patient cells. (bmj.com)

Samples were run in triplicate for all measurements, and the analysis performed using GeneScan analysis software. (nist.gov)
In T cell subset analysis, the proportion of naïve T cells and regulatory T cells (Tregs) was increased. (bvsalud.org)

Hürthle cell carcinoma. (medscape.com)
Obviously, this factor has a major impact in interpreting the natural history of this disease and adds to the controversy about the aggressiveness of Hürthle cell carcinoma. (medscape.com)
9] A study of 111 patients with Hürthle cell carcinoma found that recurrence-free survival rates were 100% in patients without vascular invasion, 95% in those with focal vascular invasion, and 77% in those with extensive vascular invasion. (medscape.com)

We found a lower level of GRHL2 expression not only in acute leukemia patients but also in cell lines when compared with normal controls. (hindawi.com)
Working in partnership with the Commonwealth of Massachusetts, Massachusetts State Public Health Laboratory, and area hospitals, the CLIA-certified lab of the Broad Institute of MIT and Harvard has begun processing COVID-19 test samples from patients across New England. (broadinstitute.org)
Serum samples were collected from non-vaccinated patients hospitalized after a SARS-CoV-2 WT infection between March and November 2020 (before the emergence of VOCs) ( Table S1 ). (frontiersin.org)
MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)
In a study of 56 patients with Hürthle cell cancer treated at Memorial Sloan-Kettering Cancer center, recurrence was a significant predictor of tumor-related mortality, and extent of invasion was the most significant predictor of outcome. (medscape.com)

Cells with rare alleles and unusual DR-DQ associations are studied. (uclahealth.org)
In 1997, DNA extracts from cells with unusual class I alleles were provided to participating laboratories. (uclahealth.org)

Mitochondrial function or the ability to generate energy through OXPHOS (oxidative phosphorylation) is vital for cell homeostasis and its dysfunction has been linked to the pathogenesis of nearly all chronic diseases ( Pieczenik and Neustadt, 2007 ). (frontiersin.org)

Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)
Adventitious viruses persistently infect three commonly used mosquito cell lines. (cdc.gov)

The number of species from where the samples are derived is constantly increasing. (wikipedia.org)

Incubation of the cells in different sera and varying serum concentrations did not indicate any significant differences between sera, but revealed a significant reduction in sensitivity if less than 15% serum was used. (cdc.gov)

Our lab prepares EBV-immortalized B-cell lines from fresh blood samples. (tau.ac.il)

That study evaluated 57 patient samples, 20 of which had different risk assessments by MammaPrint and Oncotype DX. (genomeweb.com)
Broad adapted the existing facility to process COVID-19 patient samples and accurately perform the existing CDC RT-qPCR COVID-19 diagnostic test at large scale. (broadinstitute.org)

Anatomy34

Organisms12

Diseases36

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes64

Disciplines and Occupations1

Information Science3

Genomic9

Epithelial cells13

Viability6

Tissues6

Fibroblast cell2

Tumor samples2

Tumors15

Tissue Samples3

Assays5

Proliferation4

Differentiation4

Metastatic1

Flow cytometry2

Antibody8

Mononuclear cells1

Vitro7

Characterization2

Methods6

MRNA7

Assay3

Biological samples2

Genes6

Malignant2

Type15

Biopsies1

Myeloma1

Whole genome2

Clinical samples1

Results8

Extraction1

Inflammatory cells1

Sequence1

Pathway2

20171

Strains1

Squamous1

Landline2

Origin2

Molecular3

Cellular4

Tumorigenesis1

Gene4

Cancer cell3

Migration3

Supernatant1

Data14

Prostate1

Pathways2

Inhibition1

Capillary2

Demonstrate2

Humans1

Macrophages1

Significantly higher1

Primary1

Analysis2

Carcinoma3

Patients5

Alleles2

Homeostasis1

Commonly2

Species1

Serum1

Prepares1

Patient samples2