Saccharomyces cerevisiae proteins. Medical search. Frequent questions

The yeast Saccharomyces cerevisiae is widely used to produce biopharmaceutical proteins. (dtu.dk)
Here, we increased the secretion of a heterologous α-amylase, a model protein used for studying the protein secretory pathway in yeast, by moderately overexpressing SEC16, which is involved in protein translocation from the endoplasmic reticulum to the Golgi apparatus. (dtu.dk)
I aimed to identify RNA-binding proteins (RBPs) in yeast that associate with ER and to assess their role in mRNA localization and/or translation at ER. (uni-tuebingen.de)
Herein, we used the amenable yeast Saccharomyces cerevisiae to study whether ubiquitin facilitates the expression of the fused eL40 (Ubi1 and Ubi2 precursors) and eS31 (Ubi3 precursor) ribosomal proteins. (123dok.net)
Local folding coupled to RNA binding in the yeast ribosomal protein L30. (expasy.org)
Mad1 is a non-essential protein which in yeast has a function in the spindle assembly checkpoint (SAC). (wikipedia.org)
Cell fractionation analysis as well as studies using a yeast strain that is conditionally deficient in glycosylation demonstrate that TIP1 is a heavily modified membrane‐associated protein. (researchwithrutgers.com)
Furthermore, their overexpression in wild‐type yeast enhances production of secreted proteins in S. cerevisiae, which suggests that they may be rate‐limiting factors in this process. (vtt.fi)
In accordance with the high homology level of the secretory pathway proteins in different yeast species, the polyclonal antibodies raised against S. cerevisiae Seb1p, Sso2p and Sec4p can detect homologous proteins in cell lysates of K. lactis and Pichia pastoris, the latter also in Candida utilis. (vtt.fi)
In order to construct yeast expressing vector for the tHSA-L7/L12 fusion protein, the L7/L12 ribosomal gene was amplified by PCR. (ac.ir)
Here, we test this prediction using budding yeast cells, and confirm that increasing the amount of key polarity proteins results in multiple polarity sites and simultaneous budding. (duke.edu)

Within the resolution of these immunodetection techniques, we show that proteins encoded by the SIR3, SIR4, and RAP1 genes colocalize in a statistically significant manner with Y' telomere-associated DNA sequences. (rupress.org)
In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
The SEB1/SBH1 and the SSO genes encode components of the protein secretory machinery functioning at the opposite ends, ER translocation and exocytosis, respectively, of the secretory pathway in Saccharomyces cerevisiae. (vtt.fi)
Overexpression of these genes can rescue temperature‐sensitive (ts) growth defect of many sec mutants impaired in protein secretion. (vtt.fi)
Interestingly, KlSSO1 and KlSEB1 show similar enhancing effect on production of a secreted protein as the SSO and SEB1 genes of S. cerevisiae when overexpressed. (vtt.fi)
They included genes coding for proteins with domains homologous to calcium transporters, casein kinase II, UBC13, AMSH, Vps23p, and Vps27p of Saccharomyces cerevisiae. (duke.edu)

Description: Saccharomyces Cerevisiae (strain ATCC 204508 / S288c) Thp1p protein, recombinant protein. (iowaodes.com)

The secreted recombinant fusion protein was detected in supernatant by SDS-PAGE and confirmed by western blot analysis using anti-HSA and anti-L7/L12 antibodies. (ac.ir)

AMP-activated protein kinase (AMPK) is a master metabolic regulator for controlling cellular energy homeostasis. (rcsb.org)

Pulsed-SILAC experiments reveals that NatA-deficient strains consistently increase degradation of ribosomal proteins compared to wild type. (lu.se)
To reduce the expression of a gene/protein, we need to reduce the production rate of the transcript, increase the degradation rate of the transcript, decrease the production rate of the protein, or increase the degradation rate of the protein. (biomedcentral.com)
Second, we found that protein degradation follows saturating kinetics, explaining the long half-life of Ste12 in mutants expressing elevated amounts of Ste12. (nih.gov)
Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)

Background S. cerevisiae Rad 51 protein (400 aa, 43 kDa) is a functional and structural homolog of E.coli RecA and human Rad51 proteins and plays a central role in DNA homologous recombination and recombination repair by promoting homologous DNA strand exchange reaction. (agrisera.com)

Samples: Recombinant Rad51 protein of Saccharomyces cerevisiae , analyzed by SDS-PAGE and visualized by Coomasie stain (1) , 10 ng of recombinant Rad51 protein of Saccharomyces cerevisiae (2), c rude extract of Saccharomyces cerevisiae strain BY4741 (3) were separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. (agrisera.com)
Yet, Cdc34S130AS167A expression to a level comparable to the endogenous protein led to a uniform G1 arrest at restrictive temperature, like the arrest observed in the control strain cdc34-2ts. (unimib.it)

The endoplasmic reticulum (ER) is the site of protein synthesis for secretory and membrane proteins. (uni-tuebingen.de)

The class III phosphatidylinositol 3-kinase Vps34 in Saccharomyces cerevisiae. (ruhr-uni-bochum.de)

The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae. (rupress.org)
This suggests that telomeres are clustered in vegetatively growing cells, and that proteins essential for telomeric silencing are concentrated at their sites of action, i.e., at telomeres and/or subtelomeric regions. (rupress.org)
Localization of telomeres and telomere-associated proteins in telomerase-negative Saccharomyces cerevisiae. (le.ac.uk)

Ubiquitin is a small eukaryotic protein of 76 amino acids whose name (i.e., it occurs ubiquitously) results from its remarkable evolutionary conservation [1]. (123dok.net)

It is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor of head-to-tail monomers, or as a single N-terminal moiety to ribosomal proteins. (123dok.net)
Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
Ubiquitin fusion to other r-proteins, such as P1 or P2, and even to non-ribosomal proteins such as actin, has been reported in diverse genera of rare single-celled algae as, for example, Bigelowiella [6]. (123dok.net)
The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. (ac.ir)

Finally, the moderate overexpression of SEC16 was shown to improve the secretion of two other recombinant proteins, Trichoderma reesei endoglucanase I and Rhizopus oryzae glucan-1,4-α-glucosidase, indicating that this mechanism is of general relevance. (dtu.dk)

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. (gentaur.com)
Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction. (microbiologyresearch.org)

Literature data and our computational analysis revealed that Cdc34 protein present many consensus sites for CK2 phosphorylation. (unimib.it)
Phosphorylation of the receptor protein Pex5p modulates import of proteins into peroxisomes. (ruhr-uni-bochum.de)

The proteins contain putative N‐terminal signal peptides as well as hydro‐phobic C‐terminal sequences, indicating that the proteins may be membrane bound. (researchwithrutgers.com)

The reference genome sequence of Saccharomyces cerevisiae: Then and now. (joplink.net)
A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)

In contrast to the popular SRP model, RNA seq analysis in combination with qPCR and microscopic data revealed that mRNAs coding for nuclear-related proteins can also localize to ER. (uni-tuebingen.de)
By deleting ER-associated RBPs such as Mrn1p, Whi3p, Pbp2p, Slf1p, and Ngr1p, I see an upregulation of mRNAs coding for nuclear-encoded MRPs and at the same time a loss in the protein levels of Mrpl39p, Mrpl44p, and Img2p. (uni-tuebingen.de)
A set of nuclear proteins in SACCHAROMYCES CEREVISIAE that are required for the transcriptional repression of the silent mating type loci. (jefferson.edu)
These attachment sites are linked to actin-bundles that surround the nucleus via an Ndj1-Mps3-Csm4 protein bridge that spans the inner and outer nuclear membranes. (biorxiv.org)
We have classified these motifs into three types according to their sequence similarity and have found that they are prevalent in many eukaryotic nuclear proteins in single or multiple copies. (embl.de)
Inner nuclear membrane protein LBR preferentially interacts with DNA secondary structures and nucleosomal linker. (medlineplus.gov)

Here it is demonstrated that a TIP1 homologue, TIR1, which had been previously cloned as SRP1 (serine‐rich protein), is strongly induced by a downshift in growth temperature from 30 to 10°C. We further cloned TIR2, which is transcribed at a low basal level but is increased strongly by cold shock and, to a lesser extent, by heat shock. (researchwithrutgers.com)

abstract = "TIP1 is the first known cold‐shock‐and heat‐shock‐induced gene in Saccharomyces cerevisiae. (researchwithrutgers.com)
abstract = "Background: Iwr1, a protein conserved throughout eukaryotes, was originally identified by its physical interaction with RNA polymerase (Pol) II. (johnshopkins.edu)

When telomere-associated proteins Sir2p, Sir3p and Rap1p were tagged, the same general trend was seen-Type I survivors retained the senescence/crisis state of protein localization, while Type II survivors were restored to wild type. (le.ac.uk)
In Saccharomyces cerevisiae , the telomere-associated protein Ndj1 is required for bouquet formation. (biorxiv.org)

Using pattern searches and position-dependent matrices, we have extracted the AT-hook motifs present in a non-redundant protein sequence database. (embl.de)
The HMG family of proteins comprises members with multiple HMG domains that bind DNA with low sequence specificity, and members with single HMG domains that recognize specific nucleotide sequences. (embl.de)

Biallelic hypomorphic variants in PPA2 , encoding the mitochondrial inorganic pyrophosphatase 2 protein, have been recently identified in individuals presenting with sudden cardiac death, occasionally triggered by alcohol intake or a viral infection. (nature.com)

HMG-box domains are found in one or more copies in HMG-box proteins, which form a large, diverse family involved in the regulation of DNA-dependent processes such as transcription, replication, and strand repair, all of which require the bending and unwinding of chromatin. (embl.de)
Furthermore, AT-hook motifs are frequently associated with known functional domains seen in chromatin proteins and in DNA-binding proteins (e.g. histone folds, homeodomains and zinc fingers). (embl.de)

However, the pleiotropic nature of these effects has hampered our understanding of the functional impact of protein Nt-acetylation. (lu.se)

The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy. (frontiersin.org)
Autophagy is the main cellular process responsible for degrading defective organelles and long-lived proteins. (frontiersin.org)
Vac8 controls vacuolar membrane dynamics during different autophagy pathways in Saccharomyces cerevisiae. (ruhr-uni-bochum.de)

Our data are in agreement with a role for Nt-acetylation in promoting stability for parts of the proteome by enhancing the avidity of protein-protein interactions and folding. (lu.se)
This stabilization through protein-protein interactions creates a large pool of Ste12 that is rapidly activated following pheromone stimulation. (nih.gov)
Finally, our model reveals a novel mechanism for robust perfect adaptation through protein-protein interactions that enhance complex stability. (nih.gov)
The interactions of proteins with chromosomal DNA control a variety of cellular processes, including gene transcription, DNA packing, replication, recombination, and DNA repair. (psu.edu)
High mobility group (HMG) box domains are involved in binding DNA, and may be involved in protein-protein interactions as well. (embl.de)

Our initial assays with the L. starkeyi secretome expressing differing Tr EGII domains fused to a chimeric Talaromyces emersonii - T. reesei CBHI ( TeTr CBHI) showed that the complete Tr EGII enzyme, including the glycoside hydrolase (GH) 5 domain is required for increased expression level of the fusion protein when linked to CBHI. (biomedcentral.com)
Identifying permissible limits of intracellular parameters such as protein expression provides important information for examining robustness. (biomedcentral.com)
If the target protein expression reaches the upper limit, i.e., essential cellular functions come to a halt when the level of protein exceeds a certain limit, the gene/plasmid copy number must decrease to below the upper limit. (biomedcentral.com)
In gTOW, we thus increase the expression of a protein to a limit by increasing the copy number and then determine the upper limit by measuring the corresponding copy number of the gene. (biomedcentral.com)
While gTOW is a method to measure the upper limit of protein expression, determination of the lower limit is still a question. (biomedcentral.com)
The commonly used gene-deletion experiments reduce the expression of a target protein to 0. (biomedcentral.com)
At present, there is no effective method to assess the lower expression limit of a target protein. (biomedcentral.com)
To assess the lower limit, we have to gradually reduce the expression of a protein and then measure the lower limit by some methods. (biomedcentral.com)
Many of these proteins are regulators of gene expression. (embl.de)
Expression of the L1 protein using recombinant DNA technology produces noninfectious virus-like particles (VLPs). (cdc.gov)

Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. (lu.se)

In particular, among these, S130 and S167, within the catalytic domain of the protein, are highly conserved among Cdc34 homologues in various organisms, and were identified as phosphorylated sites in vivo in a CK2-dependent manner. (unimib.it)
The encoded Kluyveromyces proteins are up to 70% identical with the S. cerevisiae homologues at the amino acid level and can functionally replace them. (vtt.fi)

Here we employ a multi-dimensional proteomics approach to analyze Saccharomyces cerevisiae lacking NatA activity, which causes global proteome remodeling. (lu.se)

The AT-hook is a small DNA-binding protein motif which was first described in the high mobility group non-histone chromosomal protein HMG-I(Y). Since its discovery, this motif has been observed in other DNA-binding proteins from a wide range of organisms. (embl.de)
It is most interesting that this motif seems to be quite specific to known or predicted chromosomal/DNA-binding proteins, suggesting that it may act as a versatile minor groove tether. (embl.de)

We have analyzed the phenotypic effects of a genomic ubi1∆ub-HA ubi2∆ mutant, which expresses a ubiquitin-free HA-tagged eL40A protein as the sole source of cellular eL40. (123dok.net)
Saccharomyces cerevisiae expresses three phospholipid hydroperoxide glutathione peroxidases. (microbiologyresearch.org)

In this study, we used the TEV protease-mediated induction of protein instability (TIPI) in combination with the genetic Tug-of-War (gTOW) to develop a method to measure the lower limit of protein level. (biomedcentral.com)
TIPI-gTOW is useful for estimating changes in the lower limit of a protein under different conditions, such as different genetic backgrounds and environments. (biomedcentral.com)
Principal Findings: Here, we identify Iwr1 in a genetic screen designed to uncover proteins involved in Pol III transcription in S. cerevisiae. (johnshopkins.edu)

However, the same improvements were not observed when Fusion 3 construct was expressed in S. cerevisiae and Y. lipolytica . (biomedcentral.com)
With purified protein and soluble substrates, the exoglucanase activity of the GH7 domain embedded in the Fusion 3 construct in L. starkeyi was significantly higher than that of the GH7 domain in TeTr CBHI expressed alone. (biomedcentral.com)
Fusion protein was purified by affinity chromatography and its amount was approximately 500 mu g/liter. (ac.ir)

2006), and Cdc34, the E2 enzyme required for the ubiquitination of many cell-cycle proteins (among which Sic1), which is phosphorylated by CK2 on Ser207, Ser216 e Ser282 (Pyerin et al. (unimib.it)
Regulation of peroxisomal matrix protein import by ubiquitination. (ruhr-uni-bochum.de)

Ubiquitin is a highly conserved small eukaryotic protein. (123dok.net)

Here, a Saccharomyces cerevisiae sod1 Δ mutant (defective for the Cu,Zn-superoxide dismutase) was found to be hypersensitive to Cr(VI) toxicity under aerobic conditions, but this phenotype was suppressed under anaerobic conditions. (microbiologyresearch.org)
In contrast, S. cerevisiae msra Δ ( mxr1 Δ) and msrb Δ ( ycl033c Δ) mutants defective for peptide methionine sulfoxide reductase (MSR) activity exhibited a Cr sensitivity phenotype, and cells overexpressing these enzymes were Cr-resistant. (microbiologyresearch.org)

Anatomy21

Organisms14

Diseases1

Chemicals and Drugs117

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes88

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care3

Yeast11

Genes6

S288c1

Recombinant fusion1

Controlling cellular1

Degradation4

Homolog1

Strain2

Membrane proteins1

Kinase1

Telomeres3

Amino Acids1

Ribosomal5

Secretion1

Escherichia2

Phosphorylation2

Putative1

Genome2

Nuclear6

Serine1

Abstract2

Telomere2

Sequence2

Mitochondrial1

Chromatin2

Functional1

Autophagy3

Interactions5

Expression10

Species1

Homologues2

Proteomics1

Chromosomal2

Expresses2

Genetic3

Fusion3

Ubiquitination2

Eukaryotic1

Defective2