• The budding yeast Saccharomyces cerevisiae has long been an outstanding platform for understanding the biology of eukaryotic cells. (microbialcell.com)
  • Robust genetics, cell biology, molecular biology, and biochemistry complement deep and detailed genome annotation, a multitude of genome-scale strain collections for functional genomics, and substantial gene conservation with Metazoa to comprise a powerful model for modern biological research. (microbialcell.com)
  • FIGURE 1: Complementary yeast-based technologies to probe SARS-CoV-2 biology. (microbialcell.com)
  • Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
  • These results describe the complexity of the cell composition, regulation and organization for this organ, and serve as an important reference map for understanding human biology and disease. (stanford.edu)
  • Model organisms such as yeast, fruit flies, and worms have advanced the study of genomics, eukaryotic biology, and evolution. (cshlpress.org)
  • Forced association of SARS-CoV-2 proteins with the yeast proteome perturb vesicle trafficking. (microbialcell.com)
  • Here we discuss three innovative applications of the yeast model system to reveal functions and investigate variants of proteins encoded by the SARS-CoV-2 virus. (microbialcell.com)
  • Directed evolution and yeast display as described by Zahradník et al generates SARS-CoV-2 proteins with enhanced binding properties or activities. (microbialcell.com)
  • The ER is the 'port of entry' for proteins destined for the cell surface and beyond. (stanford.edu)
  • Through systematic analyses, we find cell compositions that differ substantially across regions of the intestine and demonstrate the complexity of epithelial subtypes, and find that the same cell types are organized into distinct neighbourhoods and communities, highlighting distinct immunological niches that are present in the intestine. (stanford.edu)
  • Rather, lineage plasticity in bladder cancers with squamous differentiation is associated with loss of expression of FOXA1, GATA3, and PPARG, transcription factors critical for maintenance of urothelial cell identity. (bvsalud.org)
  • The microtubule cytoskeleton is critical for muscle cell differentiation and undergoes reorganisation into an array of paraxial microtubules, which serves as template for contractile sarcomere formation. (elifesciences.org)
  • We show that oMAP4 is expressed upon muscle cell differentiation and is the only MAP4 isoform essential for normal progression of the myogenic differentiation programme. (elifesciences.org)
  • Upon differentiation, mono-nucleated myoblasts withdraw from the cell cycle and fuse to form syncytial myotubes ( Wakelam, 1985 ). (elifesciences.org)
  • We also map gene regulatory differences in these cells that are suggestive of a regulatory differentiation cascade, and associate intestinal disease heritability with specific cell types. (stanford.edu)
  • Homologous recombination-deficient HRD-Dup (BRCA1 mutant-like) and HRD-Del (BRCA2 mutant-like) tumours harboured inflammatory signalling and ongoing immunoediting, reflected in loss of HLA diversity and tumour infiltration with highly differentiated dysfunctional CD8+ T cells. (bvsalud.org)
  • By contrast, foldback-inversion-bearing tumours exhibited elevated immunosuppressive TGFß signalling and immune exclusion, with predominantly naive/stem-like and memory T cells. (bvsalud.org)
  • In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
  • The Kopito laboratory seeks a molecular understanding of how cells maintain the fidelity of their proteomes. (stanford.edu)
  • This, in turn, allows the muscle cells to become very long. (elifesciences.org)
  • In this study, we used a combination of NGS, human-induced pluripotent-stem-cell-derived cardiomyocytes (iPSC-CMs) and nanopore long-read sequencing to identify the causal variant in a multi-generational pedigree of DCM. (stanford.edu)
  • Recently, the yeast model has demonstrated its utility in a perhaps unexpected area, that of eukaryotic virology. (microbialcell.com)
  • published in Microbial Cell [1] , are establishing the utility of the yeast model to address these critical questions. (microbialcell.com)
  • recombinational DNA repair (promoted during meiosis because homologous chromosomes pair at that time) and complementation (also known as heterosis , hybrid vigor or masking of mutations). (wikizero.com)
  • The Human BioMolecular Atlas Program (HuBMAP) aims to create a multi-scale spatial atlas of the healthy human body at single-cell resolution by applying advanced technologies and disseminating resources to the community. (stanford.edu)
  • Here, we leverage a set of genome-edited human PLIN2 reporter cell lines in a series of CRISPR-Cas9 loss-of-function screens, identifying genetic modifiers that influence PLIN2 expression and post-translational stability under different metabolic conditions and in different cell types. (stanford.edu)
  • The localization of individual cell types, cell type development trajectories and detailed cell transcriptional programs probably drive these differences in function. (stanford.edu)
  • Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
  • Here we carried out an integrative analysis of whole-genome sequencing, single-cell RNA sequencing, digital histopathology and multiplexed immunofluorescence of 160 tumour sites from 42 treatment-naive patients with HGSOC. (bvsalud.org)
  • Organization of the human intestine at single-cell resolution. (stanford.edu)
  • Growth defects that result are termed SPIs and indicate that the query protein in specific contexts disrupts the normal homeostasis of the cell [2] . (microbialcell.com)
  • Depletion of oMAP4 impairs cell elongation and cell-cell fusion. (elifesciences.org)
  • [7] In eukaryotes, true sexual reproduction by meiosis and cell fusion is thought to have arisen in the last eukaryotic common ancestor , possibly via several processes of varying success, and then to have persisted. (wikizero.com)
  • Cold Spring Harbor Laboratory Press (CSHLP) announced the release of T-Cell Memory, available on its website in hardcover and eBook formats. (cshlpress.com)
  • REFERENCES NOTE TO READERS OF THE CRITERIA DOCUMENTS While every effort has been made to present information in the criteria documents as accurately as possible without unduly delaying their publication, mistakes might have occurred and are likely to occur in the future. (inchem.org)
  • Planarian flatworms have "apparently limitless telomere regenerative capacity fueled by a population of highly proliferative adult stem cells. (wikipedia.org)
  • The national injection in spreading a rate or pathogen treatment radio satisfies normally to Enjoy the stages, dynamics, and subconjunctival academic channel that are progressive, or went to obtain artificial, badly that the compared images can find formed and ed. (plywoodskyscraper.com)
  • Cloning from somatic cells rather than germ cells may begin life with a higher initial load of damage. (wikipedia.org)
  • Skeletal muscles-which enable animals to move-are made up of large elongated muscle cells that span the entire length of the muscle. (elifesciences.org)
  • Here, to better understand these differences, we evaluated the organization of single cells using multiplexed imaging and single-nucleus RNA and open chromatin assays across eight different intestinal sites from nine donors. (stanford.edu)
  • Therefore, the next challenge is to understand how other processes-such as the selective stabilisation of some microtubules and the movement of cell materials along the microtubules-cooperate to control muscle fibre formation. (elifesciences.org)
  • If eutomation makes it possible to follow changes in a densely-mapped bacterial chromosome subjected to a variety of mutational situations, the resul- ting insipht: into chrenosene mechantes will be extremely va tuapics axtension Gv Ghis appreneh to ehiuccosa: ml dpbrner i her irmortene conecaucnecs Vor witereiandine a vide rane oe eilitdes pacvunetoaac gor cecil in which * good biological mechanisms of genctic recombination are available. (nih.gov)
  • COE2-2hexyl has specific effects on multiple membrane-associated functions (e.g., septation, motility, ATP synthesis, respiration, membrane permeability to small molecules) that may act together to abrogate bacterial cell viability and the evolution of drug-resistance. (pharmaceuticalintelligence.com)
  • Impeding these bacterial properties may occur through alteration of vital protein-protein or protein-lipid membrane interfaces - a mechanism of action distinct from many membrane disrupting antimicrobials or detergents that destabilize membranes to induce bacterial cell lysis. (pharmaceuticalintelligence.com)
  • Recent studies have shown that small molecules can preferentially target bacterial membranes due to significant differences in lipid composition, presence of a cell wall, and the absence of cholesterol. (pharmaceuticalintelligence.com)
  • Ss Methods: Recent evidence from gene frequency measurements (1-3) autoradiography (4) and biochemical analysis (5) demonstrate bidirectional chromosome replication in E. coli. (nih.gov)
  • This hypothesis has recently received some experimental support: (a) studies of cell division following DNA, RNA, and protein inhibition at the time of chromosome termination in synchronous cultures (16-18) in@icate that the specific replication of the lest 0.5% of the chromosome. (nih.gov)
  • blocking protein synthesis during this replication will block the subsequent cell division. (nih.gov)
  • Microvesicles loaded with MCs encoding a thymidine kinase (TK)/nitroreductase (NTR) fusion protein produced prolonged TK-NTR expression in mammary carcinoma cells. (regenerativemedicine.net)
  • The Ras family of GTPases is a collection of molecular switches that link receptors on the plasma membrane to signaling pathways that regulate cell proliferation and differentiation. (embl.de)
  • Some children have been successfully treated with blood stem cell, or bone marrow, transplants. (nih.gov)
  • NIH researchers successfully treated adults with severe sickle cell disease using a modified stem cell transplant approach that doesn't require extensive immune-suppressing drugs. (nih.gov)
  • For example, intracellular galectin-3 directly influences necrotic and apoptotic cell death pathways, as well as autophagy (Yu et al. (cd177signaling.com)
  • 2007). Galectin-3 forms cross-linked lattices with these residues that alter downstream cell signaling and inflammatory pathways (reviewed in Boscher et al. (cd177signaling.com)
  • It has been suggested that chromosome termination may trigger division by the - transcription of division related genes, located at the chromosome terminus, at the time of their replication. (nih.gov)
  • Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
  • Model organisms such as yeast, fruit flies, and worms have advanced the study of genomics, eukaryotic biology, and evolution. (cshlpress.com)
  • By contrast, foldback-inversion-bearing tumours exhibited elevated immunosuppressive TGFß signalling and immune exclusion, with predominantly naive/stem-like and memory T cells. (bvsalud.org)
  • Cloning from somatic cells rather than germ cells may begin life with a higher initial load of damage. (wikipedia.org)
  • The establishment of a large library of temperature sensitive and more completely characterized mutants covering the chromosome map thoroughly would have very many applications in the study of bacteria and especially of yeasts and higher organisms. (nih.gov)
  • We demonstrated that MCs can be loaded into shed microvesicles with greater efficiency than their parental plasmid counterparts and that microvesicle-mediated MC delivery led to significantly higher and more prolonged transgene expression in recipient cells than microvesicles loaded with the parental plasmid. (regenerativemedicine.net)
  • These results suggest that MC delivery via microvesicles can mediate gene transfer to an extent that enables effective prodrug conversion and tumor cell death such that it comprises a promising approach to cancer therapy. (regenerativemedicine.net)
  • By periodic measurement of map distances by cotransduction or interrupted mating one can monitor increases and reductions of the chromosome by the net effect of gene doubling, recombination, deletion and other processes that may affect its size. (nih.gov)
  • With a large number of standard markers and standard proce- dures the machine can keep a steady picture of the state of integration or autonomy of various plasmids, of the chromosome number, if that is subject to change, and of the size of the chromosome. (nih.gov)
  • likely that the size of the chromosome is not an accurately conserved quantity but there will be variations in the population and it is hoped that methods of measurement will be sensitive cnough to make some description of this distribution and how it changes when the parent strain of the population contains various mutations especially affecting DA replication and repair. (nih.gov)
  • We previously demonstrated that microvesicles can functionally deliver plasmid DNA to cells and showed that plasmid size and sequence, in part, determine the delivery efficiency. (regenerativemedicine.net)
  • There is no widely available cure for the inherited blood disorder sickle cell disease. (nih.gov)
  • The termination of chromosome replication appears to be necessary for chromosome segregation and subsequent septum cross wall formation (7-9). (nih.gov)
  • Almost all organisms senesce, including bacteria which have asymmetries between "mother" and "daughter" cells upon cell division, with the mother cell experiencing aging, while the daughter is rejuvenated. (wikipedia.org)
  • 27 « Glaser, Donald A. Privileged Cormunication * , 4) Genetic characterization of the chromosome terminus and the regulation of cell division in E. coli. (nih.gov)