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Capsid ProteinsCapsidVirus AssemblyVirionMolecular Sequence DataViral ProteinsCryoelectron MicroscopyAmino Acid SequenceViral Structural ProteinsDNA, ViralCell LineGenome, ViralSimian virus 40RNA, ViralAntigens, ViralVirus ReplicationAntibodies, ViralNorwalk virusBase SequenceCaliciviridaeCalicivirus, FelineSpodopteraBaculoviridaePoliovirusDependovirusGenes, ViralRNA VirusesPolyomavirusReoviridaeModels, MolecularParvovirusCaliciviridae InfectionsParvoviridaeSindbis VirusNorovirusMicroscopy, ElectronCercopithecus aethiopsPicornaviridaeOpen Reading FramesParvovirus, CanineOncogene Proteins, ViralNodaviridaeVirus UncoatingGenetic VectorsRotavirusNeutralization TestsEpitopesAphthovirusParvoviridae InfectionsIridovirusMutationBromovirusEnterovirus B, HumanRubella virusViral VaccinesHemorrhagic Disease Virus, RabbitParvovirus B19, HumanVero CellsHerpesvirus 1, HumanPapillomaviridaeRecombinant Fusion ProteinsCircovirusMinute virus of miceRecombinant ProteinsCyclophilin AInsect VirusesEnterovirus A, HumanPanicumHeLa CellsGene Expression Regulation, ViralVirosomesOrthoreovirus, MammalianInfectious bursal disease virusCloning, MolecularMosaic VirusesProtein ConformationReceptors, VirusNucleocapsidDNA Virus InfectionsSequence AlignmentProtein PrecursorsAleutian Mink Disease VirusTotivirusAmino Acid SubstitutionDNA PackagingEpitope MappingRanavirusPhylogenyCricetinaeBacteriophage T4Protein Structure, TertiaryPolyomavirus InfectionsCell NucleusEnterovirusRous sarcoma virusBluetongue virusHepatitis E virusProtein BindingSequence Homology, Amino AcidVirus Release
GenomeLarge T antigEncodesGenesStructural proteinsSimianAntigenCytoskeletal proteinsMRNASynthesisReplicationBacteriophageAdenovirusPolyAIcosahedralViral proteinHistoneChromatinVirionPeptidesSpecific proteinsCellular proteinsAntigenicNUCLEARSubunitsReceptorsEukaryoticExpressionPathogenSignal transductionNucleusPentamericAgnoproteinAntigensPolypeptideIntracellularMoleculeBindPlasmidComplementVirusVirusesStructureRegulatoryTumorDemonstrateHostGene proteinCellLentivirusCargoMajor
Genome8
  • The genome is packaged in a viral capsid of about 40-50 nanometers in diameter, which is icosahedral in shape (T=7 symmetry). (wikipedia.org)
  • The genome of a typical polyomavirus codes for between 5 and 9 proteins, divided into two transcriptional regions called the early and late regions due to the time during infection in which they are transcribed. (wikipedia.org)
  • It has been frequently reported that an intact, albeit distorted, virion particle is released from the endoplasmic reticulum into the cell cytoplasm, where the genome is released from the capsid, possibly due to the low calcium concentration in the cytoplasm. (wikipedia.org)
  • These proteins serve to manipulate the host's cell cycle - dysregulating the transition from G1 phase to S phase, when the host cell's genome is replicated - because host cell DNA replication machinery is needed for viral genome replication. (wikipedia.org)
  • The amounts of different regions of the SV40 genome present in a line of transformed mouse cells. (cshl.edu)
  • 60 serotypes, divided into seven subgroups or species (A through G). Measuring 70-90 nm, its double-stranded DNA genome is surrounded by a non-enveloped icosahedron capsid with fiber-like projections from each of its 12 vertices. (medscape.com)
  • The genes that encode proteins comprise only a tiny fraction of human DNA, even though they represent the major biological function of the genome. (healthdrugpdf.com)
  • Viral architecture is very complex, but every virus contains at least a genome and a capsid. (unboundmedicine.com)
Large T antig1
  • 5881 /codon_start=1 /locus_tag=' ' /product='nuclear localization signal of SV40 large T antigen' /note='SV40 NLS' /note=' ' /note='color: #993366' /protein_id=' ' /translation='PKKKRKV' CDS 5897. (biofeng.com)
Encodes3
Genes7
  • The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
  • Transcription of late genes encoding for structural proteins (VP1, VP2 and VP3). (expasy.org)
  • PsVs for Merkel cell polyoma virus (MCV) have been generated by co-transfection with the VP1 and VP2 genes of MCV strain 339 and a Green fluorescent protein (GFP) reporter plasmid of 293TT cells engineered to stably express the cDNA of Simian virus 40 (SV40) T antigen [ 3 ]. (biomedcentral.com)
  • It is now clear that Armadillo and beta-catenin bind directly to members of the T-cell factor/lymphoid enhancer factor subfamily of HMG box DNA-binding proteins, forming bipartite transcription factors that regulate Wingless/Wnt responsive genes in both Drosophila and vertebrates. (embl.de)
  • The genes within our cells contain the necessary information to direct the production of proteins and enzymes to support normal functions in the body. (cellculturedish.com)
  • DEFINITION Acer distylum AY-b chloroplast genes for photosystem II protein D1, psbA-trnH IGS, tRNA-His. (nig.ac.jp)
  • More common are small, subtle changes of the DNA sequence that can alter the structure and function of the proteins encoded by genes. (healthdrugpdf.com)
Structural proteins2
  • The late region contains the three capsid structural proteins VP1, VP2, and VP3, produced by alternative translational start sites. (wikipedia.org)
  • The main structural proteins of CAVEOLAE. (lookformedical.com)
Simian1
  • The term papova is derived from the first two letters of the three prototypes, papilloma virus, polyoma virus and simian vacuolating virus-40 (SV40). (biologydiscussion.com)
Antigen1
  • This modular cVLP platform exploits a split-protein (Tag/Catcher) conjugation system to enable high-density, unidirectional antigen display. (bvsalud.org)
Cytoskeletal proteins2
  • Clathrin also interacts with cytoskeletal proteins. (lookformedical.com)
  • This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS. (lookformedical.com)
MRNA2
  • Several mechanisms have been described for regulating the transition from early to late gene expression, including the involvement of the LT protein in repressing the early promoter, the expression of un-terminated late mRNAs with extensions complementary to early mRNA, and the expression of regulatory microRNA. (wikipedia.org)
  • 5-9 proteins are expressed from the two pre-MRNA by alternative splicing . (expasy.org)
Synthesis4
  • We co-opted Escherichia coli for biomolecular synthesis and assembly of this nanomaterial by expressing and maturing the EcHyd-1 hydrogenase prior to expression of the P22 coat protein, which subsequently self assembles. (nature.com)
  • Within 12h of infection and before start of DNA replication, there begins early protein synthesis. (biologydiscussion.com)
  • The presence of these proteins within virions suggests that they play a role in the early steps of lentivirus infection, prior to de novo protein synthesis directed by transcripts from the new provirus. (biomedcentral.com)
  • They damage the host cell by blocking its normal protein synthesis and using its metabolic machinery for their own reproduction. (unboundmedicine.com)
Replication4
  • Before the replication begins, early proteins are synthesized in the nucleus of the infected cells. (biologydiscussion.com)
  • Late proteins are synthesized when DNA replication is over. (biologydiscussion.com)
  • This shed light on the viral and cellular proteins that are involved in adenovirus replication. (scienceexhibitions.org)
  • The most common way of producing AAV for gene therapy involves transient transfection of three different plasmids into the cells - the transfer plasmid containing the gene of interest, the second with the rep-cap sequences for viral replication and capsid assembly, and the final helper plasmid contains the adenovirus helper functions. (cellculturedish.com)
Bacteriophage2
  • Here, we show the encapsulation and protection of an active hydrogen-producing and oxygen-tolerant [NiFe]-hydrogenase, sequestered within the capsid of the bacteriophage P22 through directed self-assembly. (nature.com)
  • Finally, we comment on a combined AFM/fluorescence application to study the influence of crowding on GFP packed within individual P22 bacteriophage capsids. (bvsalud.org)
Adenovirus2
  • Vehicle den Noordaa Clinofibrate in Amsterdam and Vehicle der Eb in Leiden utilized adenovirus and SV40 as tumor-inducing real estate agents in rodent versions. (scienceexhibitions.org)
  • discovered to correlate using the differential ramifications of the adenovirus E1 proteins for the manifestation of mobile MHC-class I substances (Bernards gene into hematopoietic stem cells (Hoogerbrugge shown an adenovirus vector missing binding sites for indigenous receptors blood-clotting elements and complement parts in its capsid. (scienceexhibitions.org)
PolyA1
  • not, the EGFP culture not helps SV40 polyA chromatin, which can Manage the estimation of RNA. (scoutconnection.com)
Icosahedral1
  • Non-enveloped capsid with a T=7d icosahedral symmetry , about 50 nm in diameter. (expasy.org)
Viral protein2
  • Many initiatives have been taken to take up various payloads selectively and precisely by surface functionalization of the outside or interior of self-assembling viral protein capsids. (bvsalud.org)
  • A very late viral protein triggers the lytic release of SV40. (ictv.global)
Histone6
  • The histone proteins get attached to DNA and results in super coiled form through winding of the DNA strands. (biologydiscussion.com)
  • BACKGROUND The histone methyltransferase (HMT) family includes histone lysine methyltransferases (HKMTs) and histone/protein arginine methyltransferases (PRMTs). (cancerindex.org)
  • A histone chaperone protein that plays a role in the deposition of NUCLEOSOMES on newly synthesized DNA. (lookformedical.com)
  • and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. (lookformedical.com)
  • A retinoblastoma-binding protein that is involved in CHROMATIN REMODELING, histone deacetylation, and repression of GENETIC TRANSCRIPTION. (lookformedical.com)
  • It is found as a subunit of protein complexes that are in involved in the enzymatic modification of histones including the Mi2 and Sin3 histone deacetylase complexes and the polycomb repressive complex 2. (lookformedical.com)
Chromatin4
  • The 48 kDa subunit, RETINOBLASTOMA-BINDING PROTEIN 4, is also a component of several other protein complexes involved in chromatin remodeling. (lookformedical.com)
  • Although initially discovered as a retinoblastoma binding protein it has an affinity for core HISTONES and is a subunit of chromatin assembly factor-1 and polycomb repressive complex 2. (lookformedical.com)
  • The repeating structural units of chromatin, each consisting of approximately 200 base pairs of DNA wound around a protein core. (lookformedical.com)
  • the hnrnp core protein antigens remain associated with the host chromatin, which appears to collapse into internal aggregates and along the nuclear envelope. (liverpool.ac.uk)
Virion3
  • After binding to molecules on the cell surface, the virion is endocytosed and enters the endoplasmic reticulum - a behavior unique among known non-enveloped viruses - where the viral capsid structure is likely to be disrupted by action of host cell disulfide isomerase enzymes. (wikipedia.org)
  • virion consists of dsDNA and protein. (biologydiscussion.com)
  • Vpx is a virion-associated protein encoded by SIV SM , a lentivirus endemic to the West African sooty mangabey ( Cercocebus atys ). (biomedcentral.com)
Peptides1
  • Bacteriophages offer several advantages as drug carriers, such as high specificity for targeting bacterial cells, low toxicity, and the ability to be engineered to express specific proteins or peptides for enhanced targeting and drug delivery. (bvsalud.org)
Specific proteins1
  • The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. (lookformedical.com)
Cellular proteins1
  • A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (lookformedical.com)
Antigenic1
NUCLEAR1
  • It was identified in a high-throughput chemical screen as inhibiting recognition of the nuclear localizing Human Immunodeficiency Virus 1 (HIV-1) integrase protein by the host heterodimeric importin (IMP) α/β1 complex, and has since been shown to bind directly to IMPα to induce conformational changes that prevent its normal function in mediating nuclear import of key viral and host proteins. (encyclopedia.pub)
Subunits2
  • Figure 1: Expression schematic showing a cartoon representation of EcHyd-1 with both subunits (hyaA and hyaB) fused to a scaffold protein encapsulated within the P22 capsid. (nature.com)
  • Capsid is made up of 72 capsomers which are built by 420 subunits. (biologydiscussion.com)
Receptors1
  • Attachement of the viral proteins to host receptors triggers lipid-mediated endocytosis of the virus into the host cell. (expasy.org)
Eukaryotic3
  • 5950 /codon_start=1 /product='2A peptide from?Thosea asigna virus capsid protein' /note='T2A' /note='Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. (biofeng.com)
  • A subset of these proteins is conserved across eukaryotic kingdoms. (embl.de)
  • The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. (lookformedical.com)
Expression4
  • Here, we describe considerations for the design of vaccine antigens with genetically fused split-protein (Tag or Catcher) binding partners and provide protocols for the expression and purification of corresponding Tag- or Catcher-AP205 cVLPs from E.coli. (bvsalud.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Expression cassettes are expressed within the cell resulting in viral proteins and a genomic ssDNA containing the expression cassette for the therapeutic gene(s). (cellculturedish.com)
  • We have generated a set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra. (bioseek.eu)
Pathogen2
  • SV40), a pathogen recognized to malaria due to P. vivax . (cdc.gov)
  • A pathogen that is composed of nucleic acid within a protein shell but can grow and reproduce only after infecting a host cell. (unboundmedicine.com)
Signal transduction1
  • Endocytic/exocytic CELL MEMBRANE STRUCTURES rich in glycosphingolipids, cholesterol, and lipid-anchored membrane proteins that function in ENDOCYTOSIS (potocytosis), transcytosis, and SIGNAL TRANSDUCTION. (lookformedical.com)
Nucleus2
  • Proteins found in the nucleus of a cell. (lookformedical.com)
  • Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (lookformedical.com)
Pentameric2
  • The viral pentameric protein complex consisting of glycoprotein H (gH)/gL/UL128-131A (PC) is considered to be an important vaccine component. (asm.org)
  • Using a subviral particle vaccine candidate, we show here that one protein complex of HCMV, termed the pentameric complex (PC), enhances the neutralizing antibody response against viral infection of different cell types. (asm.org)
Agnoprotein1
  • some examples have an additional regulatory protein called agnoprotein expressed from the late region. (wikipedia.org)
Antigens3
  • The early region usually codes for two proteins, the small and large tumor antigens, produced by alternative splicing. (wikipedia.org)
  • Capsid virus-like particles (cVLPs), assembled from viral coat proteins, are used as therapeutic cargo delivery vehicles as well as molecular scaffolds for display of vaccine antigens. (bvsalud.org)
  • Accordingly, protein antigens can be independently expressed and quality-checked prior to conjugation to pre-assembled cVLPs. (bvsalud.org)
Polypeptide1
  • Capsid contains one major polypeptide (VP1) and two identical minor polypeptide (VP2 and VP3). (biologydiscussion.com)
Intracellular3
  • Proteins which are found in membranes including cellular and intracellular membranes. (lookformedical.com)
  • Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. (lookformedical.com)
  • The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. (lookformedical.com)
Molecule1
Bind4
  • for example, SV40 LT can directly bind host cell p53, but murine polyomavirus LT does not. (wikipedia.org)
  • An educated guess is made regarding the characteristics of a gene involved in HIV resistance (for example only cell-surface proteins will be examined as they may bind HIV). (healthdrugpdf.com)
  • Proteins which bind to DNA. (lookformedical.com)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
Plasmid1
  • HPV PsVs have been produced in 293TT cells transfected with plasmid expressing L1 and L2 protein and plasmid containing the reporter gene. (biomedcentral.com)
Complement1
  • 6834 /note='SV40 poly(A) signal' /note='SV40 polyadenylation signal' /note='color: #a6acb3' rep_origin complement(7285. (biofeng.com)
Virus4
  • SV40 is similar to polyoma virus in size and structure. (biologydiscussion.com)
  • Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
  • dna binding of a 38,000-dalton herpes-simplex-virus-2-specific protein. (liverpool.ac.uk)
  • this protein has previously been shown to be a delayed early herpes simplex virus type-2 specific protein. (liverpool.ac.uk)
Viruses1
  • In this way, AFM allows obtaining nanometric resolution images of individual protein shells, such as viruses, in liquid milieu. (bvsalud.org)
Structure2
  • Tanaka, S., Sawaya, M. R. & Yeates, T. O. Structure and mechanisms of a protein-based organelle in Escherichia coli . (nature.com)
  • Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. (lookformedical.com)
Regulatory2
  • It undergoes tyrosine phosphorylation by C-SRC PROTEIN PP60 and plays a regulatory role in CAVEOLAE formation. (lookformedical.com)
  • By epub Strategisches Controlling in international tätigen Konzernen: Aufgaben - Instrumente, joint cell grids make been creating the regulatory research Protein. (scoutconnection.com)
Tumor1
  • The adenomatous polyposis coli tumor suppressor protein is also implicated in beta-catenin signaling. (embl.de)
Demonstrate1
  • By probing the infrared spectroscopic signatures and catalytic activity of the engineered material, we demonstrate that the capsid provides stability and protection to the hydrogenase cargo. (nature.com)
Host2
  • Impact of host protein dynamics on picornavirus RNA 5? (uci.edu)
  • We used the technique of X-ray crystallography to show in atomic detail how KSHV utilizes KsBcl-2 to neutralize host cell death inducing Bcl-2 proteins, including Bid and Puma, to ward off premature death of an infected cell. (mdpi.com)
Gene protein2
Cell1
Lentivirus1
  • We moreover provide On the spot Lentivirus Titration Enjoying playing cards, which enable quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. (health-es.com)
Cargo1
  • Figure 4: The P22 capsid provides protection from protease, thermal denaturation and air exposure with subsequent reactivation to its hydrogenase cargo. (nature.com)
Major1