AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaInformation ScienceHealth Care
DNA, RibosomalProtozoan InfectionsDNA, Ribosomal SpacerDNA, ProtozoanEukaryotaProtozoan ProteinsRNA, Ribosomal, 16SPhylogenyCiliophoraRNA, Ribosomal, 18SMolecular Sequence DataRNA, RibosomalRNA, ProtozoanGenes, ProtozoanRNA, Ribosomal, 5.8SSequence Analysis, DNADNA, FungalProtozoan Infections, AnimalBase SequencePolymerase Chain ReactionRNA, Ribosomal, 28SGenome, ProtozoanDNA, BacterialRNA Polymerase IEntamoeba histolyticaBacteriaPolymorphism, Restriction Fragment LengthGiardia lambliaPol1 Transcription Initiation Complex ProteinsTrypanosoma cruziLeishmaniaSpecies SpecificityAcanthamoebaCell NucleolusToxoplasmaAlveolataTetrahymena pyriformisAntiprotozoal AgentsTrypanosomaApicomplexaTrypanosoma brucei bruceiKinetoplastidaDNA PrimersGenes, rRNAIntestinal Diseases, ParasiticSirtuin 2RNA, Ribosomal, 23STrypanosomatinaSpores, ProtozoanAntigens, ProtozoanGenetic VariationTrichomonasSoil MicrobiologyAscomycotaRepetitive Sequences, Nucleic AcidTetrahymenaHartmannellaParasitesFungiSequence Homology, Nucleic AcidTranscription, GeneticCrithidiaLeishmania majorRumenTetrahymena thermophilaNucleolus Organizer RegionProtozoan VaccinesEntamoebaNucleic Acid HybridizationTrichomonas vaginalisEcosystemSilent Information Regulator Proteins, Saccharomyces cerevisiaeRNA, BacterialSequence AlignmentWater MicrobiologyDNA, HelminthDNA, PlantSarcocystidaePhysaridaGiardiasisEvolution, MolecularNaegleriaBasidiomycotaMycological Typing TechniquesLeishmania donovaniSaccharomyces cerevisiaeCloning, MolecularFresh WaterCoccidiosisCryptosporidiumAmoebozoaAmino Acid SequenceDNA, IntergenicDNA Restriction EnzymesAntibodies, ProtozoanFecesHost-Parasite InteractionsCryptosporidium parvumBlastocystis hominisDNA Fingerprinting
FungiPhylogenyBacteriaPolymerase Chain ReSpecificityApicomplexaRRNAProteinsSequencesGeneRDNAParasiteGenomeMorphologicalGeneticsSequenceGenesLeishmaniaReplicationVirusesDetectionFungalPhylogeneticFingerprintingOrganismsChromatinGiardiasisSpeciesBiologyAnalysisGenusSmallNitrogenComplementaryYeastFungusEffectsProbeOccursInternalDetectCellularSpecificCompleteField
Fungi3
  • The focus of most research on telomerase in protozoa (gloriously), fungi, and plant and animal metazoa has been on the enzyme's molecular components-once thought to consist of just a subunit of RNA and another one of protein, but now probably several of the latter. (rupress.org)
  • Herbivores rely on digestive tract lignocellulolytic microorganisms, including bacteria, fungi and protozoa, to derive energy and carbon from plant cell wall polysaccharides. (concordia.ca)
  • However, the nature of the genes encoded by eukaryotic protozoa and fungi within these environments has not been explored using metagenomic or metatranscriptomic approaches. (concordia.ca)
Phylogeny1
Bacteria4
  • Treatments had no overall effect on total bacteria (p = 0.235) and protozoa numbers (p = 0.835). (annexpublishers.co)
  • Bacteria and Archaea are all single-celled microorganisms that do not have DNA contained within a nucleus. (hawaii.edu)
  • Notable exceptions include various species of Vibrio and Legionella bacteria and protozoan parasites such as the free-living amoebae Naegleria and Acanthamoeba . (nationalacademies.org)
  • This chapter describes basic principles of ecology and evolution for waterborne viruses, bacteria, and protozoa (and yeasts and molds to a lesser extent) of public health concern as an aid to better understand how selective forces may alter one's ability to assess the microbial quality of water. (nationalacademies.org)
Polymerase Chain Re1
  • Polymerase chain reaction (PCR) can also be used to detect Pneumocystis DNA in different types of samples. (cdc.gov)
Specificity1
  • Its evolutionary success has combined the high specificity RNA-DNA base pairing for substrate recognition with a "conventional" protein-orchestrated, metal-catalyzed chemical step. (rupress.org)
Apicomplexa2
RRNA1
  • These previous metagenomic studies were based on microbiota profiles mostly identified using sequence polymorphisms of the 16S rRNA gene and therefore did not consider the functional versatility of unique rumen microbial species, nor the ability of some microbial organisms to integrate foreign DNA from other microbial organisms into their DNA. (nature.com)
Proteins8
  • It is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor of head-to-tail monomers, or as a single N-terminal moiety to ribosomal proteins. (123dok.net)
  • Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
  • Herein, we used the amenable yeast Saccharomyces cerevisiae to study whether ubiquitin facilitates the expression of the fused eL40 (Ubi1 and Ubi2 precursors) and eS31 (Ubi3 precursor) ribosomal proteins. (123dok.net)
  • We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
  • Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)
  • In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
  • Ubiquitin fusion to other r-proteins, such as P1 or P2, and even to non-ribosomal proteins such as actin, has been reported in diverse genera of rare single-celled algae as, for example, Bigelowiella [6]. (123dok.net)
  • Proteins in RIBONUCLEOPROTEIN assemblies of CRISPR-CAS SYSTEMS that function in targeting DNA of invading viruses and plasmids. (bvsalud.org)
Sequences6
  • To address the systematics of several taxonomically challenging taxa, we analyzed DNA sequences of a short portion (118-296 base pairs) of the 3′ end of the rbcL gene from three type specimens. (academic-accelerator.com)
  • However, sequences of the more variable first internal transcribed spacer portion of the ribosomal DNA repeat support its differentiation from the other species of the genera. (usda.gov)
  • Sequencing and further analysis of the HDP2 DNA fragments of 19 Asiatic isolates of T. saginata and T. asiatica indicated that the HDP2 sequences of both species exhibited clear genomic variability, due to polymorphic variable fragments, that could correspond to the non-transcribed region of ribosomal DNA. (biomedcentral.com)
  • Phylogenetic reconstructions of the Haptophyta inferred from 18S ribosomal DNA sequences and available morphological data. (supergroups.ru)
  • Consequently, established primers specifically amplifying the archaeal 16S ribosomal gene region are scarce compared to the variety of primers targeting bacterial sequences. (frontiersin.org)
  • Our aims were to: 1) optimise the recombinase polymerase amplification (RPA) assay for the isothermal amplification of the G. duodenalis β-giardin gene from trophozoites and cysts, using published primer and probe sequences, and 2) perform a pilot field validation of RPA at a field station in a resource-poor setting, on DNA extracted from stool samples from schoolchildren in villages around Lake Albert, Uganda. (lstmed.ac.uk)
Gene4
  • New gene-based technologies can now be employed to examine microbial diversity through the use of small sub-unit ribosomal DNA analysis (e.g. 16S rDNA) and to understand the function of complex microbial ecosystems in the rumen through metagenomic analysis. (nhbs.com)
  • The prevalence of B. microti was studied using the PCR method with primers complementary to the gene fragment encoding nuclear small-subunit ribosomal RNA (ss-rDNA). (edu.pl)
  • C. Graham Clark focuses mostly in the field of Protozoa, narrowing it down to matters related to Entamoeba histolytica and, in some cases, Gene. (research.com)
  • Generic and species-specific quantitative PCR methods are described, based upon the conserved 5S ribosomal RNA coding sequence of nine avian and murine Eimeria species and the Eimeria maxima MIC1 gene, respectively. (ox.ac.uk)
RDNA1
  • Results were compared to an established laboratory small subunit ribosomal RNA (SSU rDNA) qPCR with additional testing using a specific assemblage qPCR targeting the triose phosphate isomerase (tpi) DNA regions that can distinguish G. duodenalis of two different assemblages (A and B), which are human specific. (lstmed.ac.uk)
Parasite2
  • Dr. Mukhopadhyay's research interest is to understand the role of iron in metabolic disorders like insulin resistance related disorders, Neurodegenerative diseases like Parkinson disease and Alzheimer disease as well as in survival and growth of protozoan parasite leishmania donovani within macrophages that might be helpful for designing therapeutic interventions to combat these diseases. (jnu.ac.in)
  • Trichomoniasis (or "trich") is a very common sexually transmitted disease (STD) caused by infection with a protozoan parasite called Trichomonas vaginalis. (cdc.gov)
Genome2
  • And, sequencing bits of its genome, including its ribosomal DNA, Kamran Shalchian-Tabrizi of the Microbial Evolution Research Group (MERG) at the University of Oslo and colleagues discovered that it's not like anything else on earth, falling on the tree of life somewhere between single-celled parasites called excavates and amoebas. (the-scientist.com)
  • PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes. (uchicago.edu)
Morphological1
Genetics1
  • His scientific interests lie mostly in Genetics, Blastocystis, Ribosomal RNA, Phylogenetics and Entamoeba moshkovskii. (research.com)
Sequence2
  • A previously described Taenia saginata HDP2 DNA sequence, a 4-kb polymorphic fragment, was previously used as the basis for developing PCR diagnostic protocols for the species-specific discrimination of T. saginata from T. solium and for the differentiation of T. saginata from T. asiatica . (biomedcentral.com)
  • Novel microbial species in the rumen have recently been identified using metagenome-assembled genomes generated from whole metagenomic sequence data of microbial DNA from rumen samples 8 , 34 , but how their abundances are shaped by host genomics is still unknown. (nature.com)
Genes2
  • We characterised the complete mitogenome, the ribosomal cluster (including 18S and 28S) and additional genes such as Elongation factor 1 alpha (EF1α) and Histone 3. (bvsalud.org)
  • Mitochondrial DNA may also lose genes whose functions are substituted for by unrelated genes encoded in the nucleus. (biomedcentral.com)
Leishmania2
Replication2
  • My father, an accomplished architect, would have likely called the end-game of DNA replication "structurally unpleasant. (rupress.org)
  • As every well-educated graduate student knows-and some accelerated high school students too-DNA is a gorgeous dyad, but its replication is aesthetically less elegant (although as molecular biology, it is profoundly elegant). (rupress.org)
Viruses1
  • DNA, or deoxyribonucleic acid -- The primary genetic material of all cellular organisms and the DNA viruses. (nih.gov)
Detection2
  • The aim of the present study was to confirm the presence of B. microti by detection of the DNA of these protozoans. (edu.pl)
  • During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer and are reported as Relative Light Units (RLU). (cdc.gov)
Fungal3
  • DNA, Fungal" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
  • This graph shows the total number of publications written about "DNA, Fungal" by people in this website by year, and whether "DNA, Fungal" was a major or minor topic of these publications. (uchicago.edu)
  • Below are the most recent publications written about "DNA, Fungal" by people in Profiles. (uchicago.edu)
Phylogenetic1
  • His Phylogenetics research integrates issues from Zoology, DNA sequencing and Phylogenetic tree. (research.com)
Fingerprinting1
Organisms1
  • The domain Eukarya includes all organisms that have DNA contained within a nucleus. (hawaii.edu)
Chromatin1
  • In such a modified nuclear cycle, the chromatin duplicates its DNA content during the G 1 and S stages, but, instead of passing to the G 2 stage, the nucleus initiates a new G 1 phase, thus starting a new cycle of chromatin duplication. (scielo.br)
Giardiasis1
  • Giardia duodenalis is a common gastrointestinal protozoan causing 184 million cases of giardiasis worldwide annually. (lstmed.ac.uk)
Species2
  • Biodiversity of marine scuticociliates (Protozoa, Ciliophora) from China: Description of seven morphotypes including a new species, Philaster sinensis spec. (nih.gov)
  • Giardia duodenalis is a parasitic flagellated protozoan which infects a wide range of mammalian hosts, including humans, and is subdivided into at least eight genetic assemblages commonly thought to represent cryptic species. (cdc.gov)
Biology1
Analysis1
  • Ribosomal DNA restriction analysis and synthetic oligonucleotide probing in the identification of genera of lower trypanosomatids. (microbiologyresearch.org)
Genus2
Small1
  • The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
Nitrogen1
  • Drug-DNA adducts as biomarkers for metabolic activation of the nitro-aromatic nitrogen mustard prodrug PR-104A. (harvard.edu)
Complementary1
  • A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
Yeast1
  • Phospho-dependent recruitment of the yeast NuA4 acetyltransferase complex by MRX at DNA breaks regulates RPA dynamics during resection. (uchicago.edu)
Fungus1
  • Pneumocystis jirovecii used to be classified as a protozoan but is now considered a fungus. (cdc.gov)
Effects1
  • Protective effects of Alda-1, an ALDH2 activator, on alcohol-derived DNA damage in the esophagus of human ALDH2*2 (Glu504Lys) knock-in mice. (harvard.edu)
Probe1
  • The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)
Occurs1
Internal1
  • His work on Internal transcribed spacer as part of general Ribosomal RNA study is frequently connected to Subtyping, therefore bridging the gap between diverse disciplines of science and establishing a new relationship between them. (research.com)
Detect1
  • Detect as little as 0.6-1 ng of DNA per gel band with minimal to no background fluorescence. (abmgood.com)
Cellular1
  • When this RNA later leaves, its promotional job done, a little spot of single-stranded template DNA is exposed-a dangerous dangle, potentially activating chromosome and even cellular destruction. (rupress.org)
Specific1
  • Telomerase is mechanistically a reverse transcriptase but to the grateful genomes on which it operates it is a chromosome end-specific DNA polymerase. (rupress.org)
Complete1
  • To complete the 5′ end of the product DNA strand, evolution came up with telomerase (as well as a different, recombination-based mechanism that is very important in many cells and creatures but does not figure in the work we are looking at here). (rupress.org)
Field1