• Another study demonstrated that adenoviral overexpression of CARP in cardiomyocytes enhances cardiac hypertrophy induced by Angiotensin II or pressure-overload and promotes cardiomyocyte apoptosis via p53 activation and mitochondrial dysfunction. (wikipedia.org)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Ang II binds to AT 1 R, which can induce vasoconstriction, increase blood pressure, and further induce cardiac hypertrophy and proliferation of fibroblasts [15]. (researchsquare.com)
  • The final result is that, after MI, billions of cardiomyocytes (CMs) with limited proliferation capacity are lost and substituted by heterogeneous collagen-rich fibrotic scar tissue [ 2 ] [ 3 ] . (encyclopedia.pub)
  • Several other environmental factors including oxy- gen concentration and mechanical, metabolic, and bio- chemical conditions have been shown relevant in cell differentiation and have been reviewed extensively (Fig. 1) [3]. (unicyte.ch)
  • In a transgenic mouse model of cardiac-specific overexpression of CARP, mice exhibited normal physiology at baseline, but were protected against pathological cardiac hypertrophy induced via pressure-overload or isoproterenol, which could be attributed to the downregulation of the ERK1/2, MEK and TGFbeta-1 pathways. (wikipedia.org)
  • mice displayed normal cardiac function at baseline and in response to pressure overload-induced cardiac hypertrophy, suggesting that these proteins are not essential. (wikipedia.org)
  • The aim of this study was to investigate the effect and mechanism of Leonurine (Leo) against pressure-overload cardiac hypertrophy induced by abdominal aortic constriction (AAC) in rats. (researchsquare.com)
  • AT 1 R is a member of the 7-fold transmembrane G-protein-coupled receptor (GPCR) family[12,13], mainly exists in the myocardial membrane and can mediate cardiac hypertrophy induced by pressure overload and mechanical stimulation after activation [14]. (researchsquare.com)
  • CARP is highly expressed in cardiac and skeletal muscle, and is a transcription factor involved in development and under conditions of stress. (wikipedia.org)
  • Its importance has been documented in a number of physiologic and pathologic conditions including neuronal and muscle development, lymphocyte activation, cardiac hypertrophy, switching of skeletal muscle fiber type, and expression of ion channels. (pancreapedia.org)
  • A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. (molcells.org)
  • Myocardial infarction (MI) is one of the clusters of numerous other cardiovascular diseases and occurs due to the blockage of the coronary artery delivering blood (ischemia) to the ventricle with consequent oxygen shortage to the contractile cells (cardiomyocytes) [ 1 ] . (encyclopedia.pub)
  • theβ-myosin heavy chain(β-MHC)and atrial natriuretic factor (ANF), which are recognized as a marker of cardiac hypertrophy, were determined by Real-time quantitative PCR (qRT-PCR), then another gene phospholipase C (PLC), inositol triphosphate (IP 3 ), which associated with RAS were determined by Western blot(WB). (researchsquare.com)
  • Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
  • GATA4-targeted compounds 3i-1000 and 3i-1103 were identified as differential modulators of atrial and ventricular gene expression. (biomedcentral.com)
  • The effect of WNT signaling on regulation of atrial natriuretic peptide (ANP) secretion is unclear. (kjpp.net)
  • Absence of GATA4 Mutations in Moroccan Patients with Atrial Septal Defect (ASD) Provides Further Evidence of Limited Involvement of GATA4 in Major Congenital Heart Defects. (cdc.gov)
  • In cardiomyocytes treated with doxorubicin, a model of anthracycline-induced cardiomyopathy, CARP mRNA and protein levels were depleted, myofilament gene transcription was attenuated and sarcomeres showed significant disarray. (wikipedia.org)
  • Chemically induced gene expression changes were characterized by qRT-PCR, global run-on sequencing (GRO-seq) and immunoblotting, and the network of cooperative proteins of GATA4 and NKX2-5 were further explored by the examination of the GATA4 and NKX2-5 interactome by BioID. (biomedcentral.com)
  • In this line, reporter gene assays with combinatorial treatment of 3i-1000 and the BET bromodomain inhibitor (+)-JQ1 demonstrated the cooperative role of GATA4 and BRD4 in the modulation of chamber-specific cardiac gene expression. (biomedcentral.com)
  • Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
  • 4 Acute hyperglycaemia leads to chronic metabolic and haemodynamic derangements, 5 which trigger chromatin structural changes, transcription factor activation, and gene expression. (emjreviews.com)
  • Intronic Polymorphisms in Gene of Second Heart Field as Risk Factors for Human Congenital Heart Disease in a Chinese Population. (cdc.gov)
  • Platelet-derived growth factor (PDGF)-BB stimulation upregulated MCHR1 mRNA and protein levels in normal human dermal fibroblasts (NHDF), and MCHR1 silencing prevented the PDGF-BB-induced expression of the profibrotic factors transforming growth factor beta 1 (TGFß1) and connective tissue growth factor (CTGF). (bvsalud.org)
  • Myocardial hypertrophy occurs in many cardiovascular diseases. (researchsquare.com)
  • Myocardial hypertrophy is one of the important causes of death of patients caused by cardiovascular diseases and an independent risk factor for the morbidity and mortality of cardiovascular diseases [1-3]. (researchsquare.com)
  • Previous studies have shown that its development is closely related to cardiac overload[6], so abdominal aortic constriction(AAC) can use this principle to establish model of myocardial hypertrophy. (researchsquare.com)
  • The rennin-angiotensin system (RAS) has been recognized to be an important factor in myocardial hypertrophy[8]. (researchsquare.com)
  • Compounds that target tissue-specific transcription factors could overcome non-specific effects of small molecules and lead to the regeneration of heart muscle following myocardial infarction. (biomedcentral.com)
  • WNT signaling plays an important role in cardiac development, but abnormal activity is often associated with cardiac hypertrophy, myocardial infarction, remodeling, and heart failure. (kjpp.net)
  • A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. (molcells.org)
  • Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. (molcells.org)
  • Additionally, BioID analysis indicated broad interaction between GATA4 and BET family of proteins, such as BRD4. (biomedcentral.com)
  • In cardiac hypertrophy, phosphorylation-dependent functional modulation of proteins, especially sarcoplasmic reticulum (SR) and sarcomere proteins, has been demonstrated. (molcells.org)
  • It is histologically defined by renal glomerular hypertrophy, expansion of mesangial and tubular compartments, accumulation of mesangial extracellular matrix proteins, and podocytopenia associated with foot process effacement. (emjreviews.com)
  • A tight spatial and timing regulation of growth factor action during embryonic development has been suggested [4]. (unicyte.ch)
  • Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling. (cdc.gov)
  • The expression of the hub genes in the BMSCs and hepatocytes was verified by reverse transcription-quantitative PCR (RT-qPCR). (aging-us.com)
  • This indicated the involvement of epigenetic modulators in the regulation of GATA-dependent transcription. (biomedcentral.com)
  • Association of functional variant in GDF1 promoter with risk of congenital heart disease and its regulation by Nkx2.5. (cdc.gov)
  • If we allow this to continue, the results may lead to ventricular remodeling, decompensation of cardiac function and dilated cardiomyopathy, heart failure and even sudden cardiac death[7] Therefore, preventing the occurrence and development of cardiac hypertrophy is very important. (researchsquare.com)
  • Regulation of autophagy is essential to prevent unwanted and escess activation, and several pathways and molecules, both stimulatory and inhibitory, are included. (helsinki.fi)
  • More detailed structure-function analysis revealed a distinct subclass of GATA4/NKX2-5 inhibitory compounds with an acetyl lysine-like domain that contributed to ventricular cells (%Myl2-eGFP+). (biomedcentral.com)
  • Cells can communicate via physical interactions, in- cluding membrane bridge formation, such as tunneling nanotubes and cytonemes, and/or through the release of soluble factors [1-3]. (unicyte.ch)
  • CN has a number of targets but its most prominent are the NFAT (nuclear factor of activated T cells) transcription factors. (pancreapedia.org)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • The fate of the cell is deter- mined by coordinated and dynamic interactions among a number of factors, acting in a defined microenviron- ment. (unicyte.ch)
  • May also function as a paracrine antifibrotic factor in the heart. (cusabio.com)
  • Growth factors may act either in an autocrine or a paracrine fashion and their temporal and spatial concentration modulates the cell phenotype and function. (unicyte.ch)
  • Further studies have identified a role for CARP in initiation and regulation of arteriogenesis. (wikipedia.org)
  • In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
  • Cardiac hypertrophy can be characterized as the response of the heart to various hemodynamic stresses. (molcells.org)
  • Association of NKX2-5, GATA4, and TBX5 polymorphisms with congenital heart disease in Egyptian children. (cdc.gov)
  • The primary microRNA (Pri-miRNA) is produced in the cell nucleus through the transcription of a DNA strand mediated by RNA polymerase II [ 2 ]. (archivesofmedicalscience.com)
  • We found that miR-335-3p, miR-543 and the lncRNAs H19 and SMIM10L2A all play central roles in the regulation of cell cycle and DNA repair processes. (bvsalud.org)
  • Although cardiac hypertrophy is initiated by various receptors at cell membranes sensing biomechanical signals and hormones, it is generally mediated by cellular signaling cascades. (molcells.org)
  • Subsequently, β-catenin translocates to the nucleus and interacts with transcription factors including T cell factor (TCF)/lymphoid enhancer-binding factor (LEF). (kjpp.net)
  • Subsequent studies showed that CARP expression in cardiomyocytes is regulated by alpha-adrenergic signaling, in part via the transcription factor GATA4. (wikipedia.org)
  • CARP was originally identified as a YB-1-associating, cardiac-restricted transcription co-repressor in the homeobox NKX2-5 pathway that is involved in cardiac ventricular chamber specification, maturation and morphogenesis, and whose mRNA levels are exquisitely sensitive to Doxorubicin, mediated through a hydrogen peroxide/ERK/p38MAP kinase-dependent as well as M-CAT cis-element-dependent mechanism. (wikipedia.org)
  • The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
  • though in cardiomyocytes, CARP can exist as multiple alternatively spliced forms. (wikipedia.org)
  • DN is a complex multifactorial disease caused by multiple genetic and environmental factors. (emjreviews.com)
  • GATA4 molecular screening and assessment of environmental risk factors in a Moroccan cohort with tetralogy of Fallot. (cdc.gov)
  • After transcription, Pri-miRNA is cleaved by the enzymatic complex DROSHA into a micro-RNA precursor (pre-miRNA). (archivesofmedicalscience.com)