Rats mutant strains. Medical search. Frequent questions

The case for genetic monitoring of mice and rats used in biomedical re" by James R. Fahey, Hideki Katoh et al. (jax.org)
The case for genetic monitoring of mice and rats used in biomedical research. (jax.org)
Fahey JR, Katoh H, Malcolm R, Perez A. The case for genetic monitoring of mice and rats used in biomedical research. (jax.org)
The latter include long-term/carcinogenicity studies in mice and rats, a three-generation reproduction in rats and teratology studies in rabbits, dogs and monkeys. (inchem.org)
He has published several books, chapters and reviews on Mouse Genetics and Genetic Quality Controls in mice and rats. (vallhebron.com)

There is a relationship between protein aggregate structure (strain) and clinical phenotype in prion diseases, however, whether differences in the strains of α-synuclein aggregates account for the different pathologies remained unclear. (elifesciences.org)
Homozygous Prdm14 knocked-in rats have the germ cell-deficient phenotype. (mcw.edu)
Overall, the patterns of ALS gene expression were similar between the rat model and human clinical specimens, suggesting that the model would be useful for studying the phenotype of alδ/alδ mutant strains. (illinois.edu)
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)

A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. (lookformedical.com)

In biology and especially genetics, a mutant is an individual, organism, or new genetic character, arising or resulting from an instance of mutation, which is a base-pair sequence change within the DNA of a gene or chromosome of an organism resulting in the creation of a new character or trait not found in the wild type. (meddic.jp)
To create a chimaera, researchers inject stem cells from one organism into a mutant strain of a different organism. (jstor.org)

Currently, there is the potential to generate over 200,000 mutant mouse strains between existing mouse strains (over 24,000) and genetically modified mouse embryonic stem cells (over 209,000) that have been entered into the International Mouse Strain Resource Center (IMSR) from laboratories and repositories all over the world. (jax.org)
In this review, we make a case for the International Council for Laboratory Animal Science (ICLAS), which is interested in promoting and helping academic institutions develop a genetic monitoring program to bring a level of genetic quality assurance into the scientific interchange and use of mouse and rat genetically mutant models. (jax.org)

After a genetic revolution in the 80s, mice overtook rats as the laboratory animal of choice for many researchers. (rrrc.us)
Rats, Inbred WF are a strain of laboratory rats that are commonly used in medical research due to their consistent genetic makeup and susceptibility to various diseases. (lookformedical.com)
Laboratory studies found that the samples had neutralising levels of antibodies which appeared to work against N501Y in the new strains. (yorkshireeveningpost.co.uk)
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C . (yeastgenome.org)

We show via an in vitro binding assay that miR-9a binds to sNPFR1 mRNA in insect cells and to the mammalian orthologue NPY2R in rat insulinoma cells. (nature.com)
Foscarnet does not require activation (phosphorylation) by thymidine kinase or other kinases and therefore is active in vitro against HSV TK deficient mutants and CMV UL97 mutants. (druglib.com)
Here, we described helpful modification to the current strategies and established a tractable RG system for the rescue of the simian RRV strain, the human CDC-9 strain and a murine-like RV strain, which is suitable for both in vitro and in vivo studies. (cdc.gov)
Test chemical did not induce mutation in mammalian cell line in the presence and absence of metabolic activation and hence it is not likely to classify as a gene mutant in vitro. (europa.eu)
Taken together, these findings suggest that a rapid, sensitive, non-invasive, and homogeneous phenotypic assay has been developed for assessing the antiviral agent susceptibility of mutant viruses that emerge from in vitro resistance Morin Hydrate selection studies. (mdmpathway.com)

Over the years, while permanent inbreeding effectively eliminates a proportion of new mutant alleles, another undetected fraction may become progressively fixed in the homozygous state, replacing the original allele, a process known as genetic drift. (vallhebron.com)
Curated mutant alleles for the specified gene, listed alphabetically. (yeastgenome.org)

The aim of this study was to characterize the pacemaker activity and inhibitory neurotransmission in the colon of Ws/Ws mutant rats, which harbor a mutation in the c- kit gene that affects development of interstitial cells of Cajal (ICC). (mcmaster.ca)
In some organisms mutants can be created by gene targeting to assess the function of any given gene. (meddic.jp)
This congenic strain was established as a strain with the genetic background of WKY onto which a segment from the BUF/Mna containing the proteinuria-susceptible gene, Pur1 (on chr.13, Murayama, 1998) has been transferred. (kyoto-u.ac.jp)
The strain having a Endonuclease-induced mutation in Il2rg gene was established by TAL effector nuclease obtained from Dr. Yamamoto at Hiroshima University. (kyoto-u.ac.jp)
This strain shows severe combined immunodeficiency caused by a 7-bp deletion in the exon 2 of Il2rg gene. (kyoto-u.ac.jp)
However, Haemophilus influenzae type b strain Eagan (Hib), a causative agent of bacterial sepsis and meningitis in young children, disrupted in its hktE gene is not attenuated in virulence, and retains the ability to rapidly scavenge H 2 O 2 . (biomedcentral.com)
They routinely provide mouse and rat model development services using all standard approaches such as gene targeting, the generation of chimeric mice from gene-targeted ES cells and gene editing technologies such as TALENs or CRISPR/Cas9. (infrafrontier.eu)
ALS gene expression was studied in the hyposalivatory rat model of oral candidiasis and in clinical specimens collected from HIV-positive patients to assess similarities in expression patterns between the model system and clinical isolates. (illinois.edu)
To address the question of specificity for the allele encoding the Na V 1.4-R669H variant as a cause of HypoPP and to produce a model system in which to characterize functional defects of the mutant channel and susceptibility to paralysis, we generated knockin mice carrying the ortholog of the gene encoding the Na V 1.4-R669H variant (referred to herein as R669H mice). (jci.org)

Thirteen S. enterica strains were selected according to high and low virulence phenotypes. (bvsalud.org)

Mutation of Plekha7 attenuates salt-sensitive hypertension in the rat. (mcw.edu)
The test chemical did not induce mutation in the Salmonella typhimurium strains both in the presence and absence of S9 metabolic activation system and hence is not likely to be mutagenic under the conditions of this study. (europa.eu)

Mutants should not be confused with organisms born with developmental abnormalities, which are caused by errors during morphogenesis. (meddic.jp)

There are many cognitive and physiological characteristics that make the rat an ideal human disease model in research areas such as neurobiology, cardiobiology, and immunology. (infrafrontier.eu)

2000), so that as the different parts of penicillin biosynthesis operons in and various other antibiotic-producing strains of includes three open up reading structures encoding SulP polypeptides. (exposed-skin-care.net)

To study the role of PGdx in virulence and to assess the requirement of H 2 O 2 -scavenging during the course of infection, both a pgdx single mutant and a pgdx / hktE double mutant of Hib were assayed for virulence in an infant rat model. (biomedcentral.com)
Finally, H 2 O 2 -scavenging is not a determinant of Hib virulence in the infant rat model of infection. (biomedcentral.com)

Targeted ES cells were injected into Crlj:WI blastocysts to produce chimeric rats. (mcw.edu)
The chimeric rats were crossed with Crlj:WI rats to produce heterozygous founder rats. (mcw.edu)
These rat strains are being maintained by crossing the founder rats with Crlj:WI rats. (mcw.edu)

or each of these agents, carcinogenicity in rats and/or mice, els, differences in exposure con- there was sufficient evidence of car- for example for the liver (aflatoxins, ditions between studies in animals cinogenicity from studies in rats and/ trichloroethylene [TCE], and vinyl and in humans, or limitations in Part 1 · Chapter 1. (who.int)

Traditional phenotypic assays selleck chemicals used to assess the susceptibility of mutant human immunodeficiency virus type-1 (HIV-1) obtained from infected patients or from resistance selection to antiviral agents in cell culture are rather tedious and time consuming. (mdmpathway.com)

Knockout rats via embryo microinjection of zinc-finger nucleases. (mcw.edu)
[ 3 ] For example, a collection of knockout-moss mutants can be used to identify genes with so far unknown functions. (meddic.jp)

The RRRC would like to acknowledge the important contributions to science and medicine made possible by the use of rats in biomedical research. (rrrc.us)
Access to this free-of-charge-service will be granted on the basis of the applicant's research plans and the potential impact of the proposed novel rat model on the wider biomedical research community. (infrafrontier.eu)

They are now seeking to patent the concept of using the mutant virus as a vaccine candidate for HMPV as it showed efficacy in protecting rats against HMPV infection. (drugtargetreview.com)
The UK's chief scientific adviser Sir Patrick Vallance said it could be the case that the new strains reduce the efficacy of vaccines, but more studies were needed. (yorkshireeveningpost.co.uk)

A strain of Rattus norvegicus with elevated blood pressure used as a model for studying hypertension and stroke. (lookformedical.com)

Using this RG system, we successfully recovered the simian RV RRV strain, the human RV CDC-9 strain, a reassortant between murine RV D6/2 and simian RV SA11 strains, and several reassortants and reporter RVs. (cdc.gov)

Spontaneous activity of nitrergic nerves caused sustained inhibition of muscle activity in both wild-type (+/+) and Ws/Ws rats. (mcmaster.ca)
Nephrotic syndrome in spontaneous thymoma rats, Buffalo/Mna. (kyoto-u.ac.jp)
A strain of Rattus norvegicus used as a normotensive control for the spontaneous hypertensive rats (SHR). (lookformedical.com)
A strain of Rattus norvegicus which is a model for spontaneous insulin-dependent diabetes mellitus (DIABETES MELLITUS, INSULIN-DEPENDENT). (lookformedical.com)

The Rat Strain Ontology (RS) is currently being developed at the Rat Genome Database. (mcw.edu)
Here, we report the draft genome sequences of C. botulinum group II strains 202F (serotype F) and Hazen (serotype E). The genomes share many similarities, including multiple mobile genetic elements. (bvsalud.org)
Recent progress in genome-editing technology will be used to develop many new rat mutants - in order to model human diseases which cannot be treated effectively today. (infrafrontier.eu)

After the treatment, the mouse embryo began to develop a rat pancreas. (jstor.org)
For example, in one previous experiment, researchers injected rat stem cells into the embryo of a mouse that lacked the ability to grow a pancreas. (jstor.org)

Following incubation with 35 S-captan, the rate of 35 S binding and the percentage of 35 S-labelled gas evolved were slightly higher in isolated liver nuclei from normal rats than from partially hepatectomized rats (Couch et al 1977). (inchem.org)

The RRRC is fully operational at this time and we are excited to be able to assist researchers with all their rat-related needs. (rrrc.us)
Researchers who created a mutant form of human metapneumovirus (HMPV) , lacking its typical m6A methylation, have demonstrated that this mutant strain results in both an innate and adaptive immune response. (drugtargetreview.com)
The pharmaceutical giant and researchers from the University of Texas Medical Branch carried out lab tests on the strains, one of which was found in the UK while the other originated in South Africa . (yorkshireeveningpost.co.uk)
In a new Trans-national Access Call INFRAFRONTIER offers open access to its infrastructure and expertise for researchers who need rat mutant models to be generated for their scientific aims. (infrafrontier.eu)

In Ws/Ws rats, the density of KIT-positive cells was markedly reduced. (mcmaster.ca)
In summary, loss of ICC markedly affects pacemaker and motor activities of the rat colon. (mcmaster.ca)
This has markedly diluted the original strain. (lookformedical.com)

Identification and Characterization of Novel Rat Polyomavirus 2 in a Colony of X-SCID Rats by P-PIT assay. (kyoto-u.ac.jp)

In +/+ rats, chronic stress induced barrier dysfunction in the ileum and colon (increased macromolecular permeability and depletion of mucus) and ultrastructural changes in epithelial cells (enlarged mitochondria and presence of autophagosomes) associated with bacterial adhesion and penetration into enterocytes. (mcmaster.ca)
In intestine of Ws/Ws rats, epithelial function and morphology were unchanged by chronic stress, bacterial-epithelial cell interaction was not demonstrated, and there was no evidence of inflammatory cell infiltration. (mcmaster.ca)
Bacterial growth was observed in the duodenum compartment after 180 min for all but one strain and growth continued into the ileal compartment. (bvsalud.org)

In cotton rats the mutant HMPV functioned like a vaccine, according to the research, preventing infection and triggering both a strong innate immune response and an effective adaptive response. (drugtargetreview.com)
Two Candida albicans strains, SC5314 and OY-2-76, were used in the rat model system and infection progressed for 3 or 5 days. (illinois.edu)
Expression of ALS1, ALS2, ALS3 and ALS4 was observed in rats infected for 3 days with ALS5 and ALS9 transcripts detected after 5 days of infection. (illinois.edu)

Research has shown that human metapneumovirus uses RNA methylation to hide from the immune system and that knocking out this methylation creates a mutant strain which acts like a vaccine. (drugtargetreview.com)
We proved a concept that these mutant viruses are improved vaccine candidates for HMPV. (drugtargetreview.com)
The Covid-19 vaccine from Pfizer and BioNTech appears to effectively protect against mutant strains of the virus, new research suggests. (yorkshireeveningpost.co.uk)
The findings indicate that the vaccine will work against the two mutant variants of the virus that are currently causing rapid spread across the UK, forcing lockdown restrictions to be significantly tightened. (yorkshireeveningpost.co.uk)
Pfizer has now tested 16 different mutations in the strains and found that none of them have had any impact on how the vaccine works. (yorkshireeveningpost.co.uk)
We recently reported a lack of interference between inactivated rotavirus vaccine (IRV) and inactivated poliovirus vaccine (IPV) and their potential dose sparing when the two vaccines were administered intramuscularly either in combination or standalone in rats and guinea pigs. (cdc.gov)
This improved system represents an important tool and great potential for the rescue of other hard-to-recover RV strains such as low replicating attenuated vaccine candidates or low cell culture passage clinical isolates from humans or animals.IMPORTANCE Group A rotavirus (RV) remains as the single most important cause of severe acute gastroenteritis among infants and young children worldwide. (cdc.gov)
We have prepared a human rotavirus vaccine strain CDC-9 (G1P[8]) which when grown in MA104 cells to passages 11 or 12 (P11/P12), had no nucleotide and amino acid sequence changes from the original virus in stool. (cdc.gov)

When the modification was blocked by the team, the resulting mutant virus activated a stronger-than-normal innate immune response which allowed the development of an adaptive response as well. (drugtargetreview.com)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

A genetic locus susceptible to the overt proteinuria in BUF/Mna rat. (kyoto-u.ac.jp)
Click "Sequence Details" to view all sequence information for this locus, including that for other strains. (yeastgenome.org)

A test item is considered as a mutagen if a biologically relevant increase in the number of revertants exceeding the threshold of twice (strains TA 98, TA 100, and WP2 uvrA) or thrice (strains TA 1535 and TA 1537) the colony count of the corresponding solvent control is observed. (europa.eu)

We are using a zebrafish model of Duchenne muscular dystrophy (the dmd mutant strain) to investigate the developmental biology and epigenetics of DMD as well as to identify new drug therapies for DMD. (seattlechildrens.org)

INFRAFRONTIER will also provide open access to all newly developed disease models through the European Mouse Mutant Archive (EMMA). (infrafrontier.eu)

Rats, Inbred Strains" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (wakehealth.edu)

This combines been designed January 2016 and has under rat. (isak-rubenchik.de)

It's the first time that the European research infrastructure for modeling human diseases via mammalian genomes expands its unique services to generate not only mouse, but rat models for its users. (infrafrontier.eu)

Further examination indicated that structural differences in the C-terminal region of α-synuclein strains lead to different effects on proteasome activity. (elifesciences.org)
Most mouse users are not fully aware that inbred strains of mice (same for rats) are actually distributed in "families" of related substrains that stem from a common ancestral strain and that these present several genetic differences. (vallhebron.com)

To improve the efficiency of this process, a novel method was developed in which mutant viruses are captured with magnetic nano-beads and used to infect gag-GFP reporter cells to evaluate the extent of resistance conferred by the mutant viruses against antiviral agents. (mdmpathway.com)

Here, we describe useful modifications to the RG system that significantly improve rescue efficiency of multiple RV strains. (cdc.gov)

One α-synuclein fibril induced marked accumulation of phosphorylated α-synuclein and ubiquitinated protein aggregates, while the other did not, indicating the formation of α-synuclein two strains. (elifesciences.org)
Effects on Other Biochemical Parameters Incubation of isolated liver nuclei from normal rats with captan for 10 minutes at 37 C resulted in changes in distribution of various protein fractions. (inchem.org)

Mammalian neuropeptide Y receptors (NPYRs) show significant homology with sNPFR1 (ref. 7 ) and treatment of rat insulinoma cells with the mammalian sNPF orthologue NPY enhances their expression of insulin 5 . (nature.com)

We also work closely with Dr. David Mack's lab ( https://iscrm.uw.edu/faculty/david-l-mack/ ) to take advantage of human iPSC and rat models of DMD. (seattlechildrens.org)
We also work closely with Dr. David Mack's lab ( https://iscrm.uw.edu/faculty/david-l-mack/ ) and their human iPSC and rat models of DMD. (seattlechildrens.org)
When the knock-out mutant was introduced to human cells, it triggered type I interferon production - a signal the innate immune system was active. (drugtargetreview.com)
Lettuce inoculated separately with each S. enterica strain was used as food matrix in the TNO gastrointestinal model (TIM-1) of the human upper gastrointestinal tract. (bvsalud.org)
So rat models will advance knowledge of human diseases and certainly be of widespread use in biomedical science. (infrafrontier.eu)
The STAS domain name shown may be the backbone framework of the human being pendrin STAS domain name encompassing aa 515C734 (excluding the intervening series (IVS) area of aa 566C653 between helix 1 and strand 3), as modeled with PyMOL through the X-ray crystal framework of rat SLC26A5/prestin (PDB Identification 3LLO). (exposed-skin-care.net)

April 4, 2023 is World Rat Day! (rrrc.us)

Results from this experiment were confirmed by those of a second test with groups of female mice of the same strain fed dietary levels of captan ranging from 500 to 16 000 ppm for up to 213 days. (inchem.org)
In experiment I without S9 mix, the data in the solvent control of strain TA 100 were slightly above our historical control range. (europa.eu)

MTS510 mAb detects TLR4-MD-2 on the surface of thioglycollate-elicited macrophages from all mouse strains tested (ie, BALB/c, C57BL/6, C3H/HeJ, C3H/HeN, and DBA/1), including the C3H/HeJ strain which expresses an LPS-resistant mutant TLR4. (bdbiosciences.com)
INFRAFRONTIER ® and European Mouse Mutant Archive - EMMA ® are registered trademarks at the European Union Intellectual Property Office (EUIPO). (infrafrontier.eu)

Wild-type, but not Ws/Ws, rats showed low- and high-frequency cyclic depolarization that were associated with highly regular myogenic motor patterns at the same frequencies. (mcmaster.ca)
In Ws/Ws rats, irregular patterns of action potentials triggered irregular muscle contractions occurring within a bandwidth of 10-20 cycles/min. (mcmaster.ca)

Electrical field stimulation of enteric nerves, after blockade of cholinergic and adrenergic activity, elicited inhibition of mechanical activity and biphasic inhibitory junction potentials both in wild-type and Ws/Ws rats. (mcmaster.ca)
METHODS: Mast cell-deficient (Ws/Ws) rats and wild-type control (+/+) rats were submitted to water avoidance stress or sham stress (1 h/day) for 10 consecutive days, as a model of ongoing life stress. (mcmaster.ca)
In the case of cotton rats, the mutant virus produced a higher amount of type I interferon and triggered a higher antibody response and a higher T-cell immune response. (drugtargetreview.com)
There will be other new mutants and we will need to monitor the situation carefully by repeating this type of study on new variants as they appear. (yorkshireeveningpost.co.uk)

The strains produced similar oral lesions at 3 days. (illinois.edu)

TOXICOLOGICAL STUDIES Special Studies on Reproduction Groups of 15 male and 30 female rats (Charles River COBS CD strain, 31 days old) were fed technical captan in their diet at the equivalent of 0, 25, 100, 250 or 500 mg/kg body weight/day for 102 days and then mated to initiate a three-generation reproduction study. (inchem.org)
In the present study, we optimized the formulations of both vaccines and investigated the feasibility of manufacturing a combined IRV-IPV dissolving microneedle patch (dMNP), assessing its compatibility and immunogenicity in rats. (cdc.gov)

Rats bearing mutant genes which are phenotypically expressed in the animals. (lookformedical.com)

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