Other sources of RNase contamination can be bacterial cultures grown for cloning, or products, such as restriction enzymes. (severnbiotech.com)
Airborne or surface and solution contamination can also be a problem and the laboratory water supply is an obvious environmental source of RNase. (severnbiotech.com)
The assay, which we show is amenable to multiplexed detection in a single lateral-flow strip incorporating an internal control for ribonuclease contamination, should facilitate SARS-CoV-2 detection in settings with limited resources. (nature.com)
RNase contamination is an exceptional concern when working with RNA. (biotechrabbit.com)
Blot analysis3
These applications include next-generation sequencing, transfection, real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR, northern blot analysis, S1 nuclease assays, RNase protection assays, and cDNA library preparations. (thermofisher.com)
The RiboGreen reagent is ideal for fast and accurate measurement of RNA before generating cDNA, northern blot analysis, S1 nuclease assays, RNase protection assays, reverse transcription PCR, and differential display PCR. (thermofisher.com)
Relative to other techniques for measuring mRNA, such as Northern blot analysis, RNAse protection assays, or in situ hybridization, RT-PCR is significantly more robust at detecting the RNA transcript of any gene regardless of its relative abundance. (stratech.co.uk)
QPCR3
The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, Northern blotting, nuclease protection assays, cDNA library preparation after poly (A)+ selection, and so on. (shoujiuji.com)
Order ready-to-use PrimeTime™ qPCR Assays or you can design your own custom assay. (idtdna.com)
Familiarize yourself with critical parameters of qPCR assay design, specificity analysis, data interpretation, and troubleshooting. (idtdna.com)
Enzyme5
This Sterile Highly Purified Water product is DEPC treated and manufactured to eliminate RNase enzyme activity. (severnbiotech.com)
Here, we report the clinical validation of the specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) assay using the enzyme Cas13a from Leptotrichia wadei for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-the virus that causes coronavirus disease 2019 (COVID-19)-in 154 nasopharyngeal and throat swab samples collected at Siriraj Hospital, Thailand. (nature.com)
The enzyme is purified from a recombinant E. coli strain carrying the RNase Inhibitor gene. (biotechrabbit.com)
One unit is defined as the amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A (hydrolysis of cyclic cytidine-monophosphoric). (biotechrabbit.com)
In Drosophila melanogaster , the RNase III-like enzyme Dicer cleaves the dsRNA silencing trigger into RNA species of approximately 21 to 25 nucleotides. (biomedcentral.com)
MRNA3
RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. (delos.info)
Total RNA was extracted and probed for IL-2 mRNA by ribonuclease protection assay. (biomedcentral.com)
RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. (thermofisher.com)
Pipette tips2
The inclusion of a good SOP (standard operating procedures) and the use of certified RNase-free reagents and equipment especially pipette tips contribute to removal of RNase. (severnbiotech.com)
DEPC-treat all tubes and pipette tips or use certified nuclease-free labware with aerosol filters. (biotechrabbit.com)
RNAi2
RNA synthesized from this kit is suitable for many applications including RNA structure and function studies, ribozyme biochemistry, probes for RNase protection or gel shift assays, hybridization-based blots, anti-sense RNA or RNAi experiments, microarray analysis, microinjection, sgRNA synthesis and in vitro translation studies. (neb.com)
RNAi pathways are triggered by double-stranded RNA (dsRNA) processed into sRNA duplexes by Dicer ribonucleases [ 1 ] [ 2 ] . (encyclopedia.pub)
DNase3
RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. (delos.info)
货号： 10977015 规格 Purity or Quality Grade:DNase-Free, RNase-Free, Protease-Free, Molecular Biology GradeSample Treatment:Not DEPC-TreatedFormat:Bottle(s)Product Size:500 mLPurification:Membrane-FilteredGreen Features:Sustainable packagingShipping Condition:Room Temperature 内容及储存 Storage: Room Temperature 描述 UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. (ujmoreway.com)
It is 0.1-µm membrane-filtered and tested for DNase and RNase activity.Performance and quality testingNo DNase, RNase, or protease acti. (ujmoreway.com)
Vitro2
The RNase Inhibitor helps to prevent RNA degradation in applications like cDNA synthesis, RT-PCR, in vitro transcription/ translation reactions or RNA purification. (biotechrabbit.com)
The TPP1/POT1 heterodimer also promotes telomerase processivity, as demonstrated by an in vitro direct telomerase activity assay [ 4 , 20 ]. (prolekarniky.cz)
Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. (delos.info)
The assays are many times more sensitive than conventional UV absorbance and can be optimized for different concentration ranges. (thermofisher.com)
The recommended final concentration for RNA protection is about 1 -2 units of RNase Inhibitor for every 1 µl of the reaction mixture. (biotechrabbit.com)
Whole blood samples are first lysed and homogenized in a strong denaturant and phenol containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. (shoujiuji.com)
Cleaves1
DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. (lookformedical.com)
Amenable1
This simple and convenient single-tube design works well when performing a small number of assays and is amenable to high-speed, high-throughput applications. (stratech.co.uk)
Synthesis1
To prevent RNA from degradation, add Ribonuclease inhibitor (optional) in to the cDNA synthesis reaction (20 units for 20 µl reaction). (biotechrabbit.com)
Gene6
Nuclease protection assays are used to map introns and 5' and 3' ends of transcribed gene regions. (wikipedia.org)
Other techniques include dot blotting, ribonuclease protection assays (RPAs), serial analysis of gene expression (SAGE), and differential or subtractive hybridization. (idtdna.com)
The standard in single cell gene expression The Chromium X, powered by Next GEM technology, can run the full breadth of single cell assays. (mosaiquedessavoirs.eu)
This approach provides a reliable and potential tool, not only for plant protection against virus diseases, but also for the study of gene silencing mechanisms in plant virus infections. (biomedcentral.com)
Expression of the pDNA (R70pRomiLuc)-derived gene was investigated in cod tissues and cultures of cod aEEC by means of real-time RT-PCR and luciferase activity assay. (silverchair.com)
Transcription1
and we have recently begun to study effects of chronic TNF on chromatin remodelling across the IL-2 proximal promoter (pIL-2), which precedes initiation of transcription, using the chromatin accessibility real-time PCR (CHART-PCR) assay [ 3 ]. (biomedcentral.com)
Digestion2
Nuclease protection assay products are limited to the size of the initial probes due to the destruction of the non-hybridized RNA during the nuclease digestion step. (wikipedia.org)
Nuclei were isolated and subjected to limited nuclease digestion. (biomedcentral.com)
Routinely2
PicoGreen reagent assays are routinely used for genotyping by allele-specific PCR, quantitating dsDNA samples before and after PCR amplification, and determining PCR amplification yields before sequencing. (thermofisher.com)
OliGreen reagent assays are routinely used for antisense oligonucleotides, aptamers, genomic DNA isolated under denaturing conditions, PCR primers, sequencing primers, and single-stranded phage DNA. (thermofisher.com)
Single-stranded1
The mixture is then exposed to ribonucleases that specifically cleave only single-stranded RNA but have no activity against double-stranded RNA. (wikipedia.org)
Downstream1
The high lysing efficiency of the Bead Ruptor Elite results in an increase in nucleic acid, protein and small molecules yields and increases the sensitivity of the downstream assay. (fliphtml5.com)
Biochemistry1
Nuclease protection assay is a laboratory technique used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample extracted from cells. (wikipedia.org)
Chromosome2
In this study, we have identified TIN2-R282H, the most common DC-causing mutation in shelterin subunit TIN2, as a separation-of-function mutant which impairs telomerase recruitment to telomeres, but not chromosome end protection. (prolekarniky.cz)
Notably, mutations in the TPP1 OB-fold domain compromise telomerase-dependent telomere extension but not telomere end protection [ 18 , 21 ], indicating that TPP1 performs a role in telomerase regulation which is distinct from its contribution to chromosome end protection. (prolekarniky.cz)
Laboratory1
RNase A, providing most threat to RNA integrity, is a highly stable contaminant of any laboratory. (biotechrabbit.com)
Stability1
We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
Rapid1
PicoGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. (thermofisher.com)
Activity1
RNase activity was not observed after agarose gel electrophoresis. (biotechrabbit.com)
Extraction1
However, RNase A treatment of positive samples prior to RNA extraction did not degrade viral RNA, indicating the presence of SARS-CoV-2 nucleocapsids or complete virus particles protecting the RNA as opposed to free viral RNA. (biomedcentral.com)