• Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • MTT colorimetric assays, colony formation assays and 5‑ethynyl‑20‑deoxyuridine incorporation assays were also conducted to evaluate ACC cell proliferation. (cancerindex.org)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • Antibacterial activity of the ethanol extract of D. spinosa leaves was tested by disc diffusion assay and broth macrodilution assay. (ijpsr.com)
  • The extract showed antibacterial activity against a wide range of Gram-positive and Gram-negative bacteria in both assays. (ijpsr.com)
  • Antibacterial activity test by Disc Diffusion Assay: Sterile blank discs (BBL, Cocksville, USA) were impregnated with the test extract at the concentration of 500 μg/disc. (ijpsr.com)
  • The active compound curcumin is believed to have a wide range of biological effects including anti-inflammatory, antioxidant, antitumor, antibacterial, and antiviral activities, which have shown a lot of potential for use in clinical medicine. (blogspot.com)
  • Extracts were assayed for antibacterial activity using the agar well diffusion and micro plate dilution assay. (biomedcentral.com)
  • In addition, minimum bactericidal concentrations (MBC), lactate dehydrogenase (LDH) release assay and rhodamine 6G intake assay were used to ascertain the antibacterial activity. (biomedcentral.com)
  • Antioxidant, cytotoxic and antinociceptive activities were screened from spikes 3. (ijpsr.com)
  • We then screened a patient-derived xenograft library to identify SCLC as a tumor type with enhanced sensitivity to calicheamicin ADCs. (aacrjournals.org)
  • Reciprocal editing of cells generally produced reciprocal effects on tumorgenicity in xenograft assays. (nature.com)
  • Epidemiological and clinical studies have suggested that cancer could be prevented or significantly reduced by treatment with anti-oxidant and anti-inflammatory drugs, therefore, curcumin, a principal component of turmeric (a curry spice) showing strong anti-oxidant and anti-inflammatory activities, might be a potential candidate for the prevention and/or treatment of cancer and other chronic diseases. (blogspot.com)
  • These data identify novel therapeutic vulnerabilities and may inform future trials of IGF inhibitory drugs. (nature.com)
  • Outcomes for patients with melanoma have improved over the past decade because of the clinical development and Food and Drug Administration (FDA) approval of immunotherapies targeting checkpoint receptors such as programmed death-1 (PD-1), programmed death ligand 1 (PD-L1) or cytotoxic T lymphocyte antigen-4 (CTLA-4). (biomedcentral.com)
  • H3.3 mutant gliomas are also particularly sensitive to NOTCH pathway gene knockdown and drug inhibition, reducing their viability in culture. (nature.com)
  • Under the tumor immunotherapy strategy, various monoclonal antibody drugs are being developed and marketed to eliminate tumor cells by suppressing tumor immune-checkpoint signaling pathways and reactivating antitumor immune responses. (thno.org)
  • Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
  • Diagnostic testing continues to improve, but multi-drug resistance to treatment is an increasing challenge. (cdc.gov)
  • Therapies tailored to specific genetic lesions and diagnostic tests that assay for their respective molecular targets are now an established part of clinical practice across various tumour types, including chronic myeloid leukaemia 1 , gastrointestinal stromal tumours and epithelial tumours, such as breast and colon cancer 2 . (ersjournals.com)
  • Many diagnostic and prognostic assays have begun to show clinical applicability providing additional tools to optimize and individualize treatments. (biomedcentral.com)
  • Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)
  • METHODS: A drug array was used to recognize compounds that can suppress the cisplatin-induced and radiation-induced PD-L1 expression in NSCLC via the flow cytometry-based assay. (bvsalud.org)
  • Open data on the gene expression of the NCI-60 cell lines, provides a unique opportunity to add another dimension to the preclinical development of such drugs by interrogating correlations with gene expression patterns. (biomedcentral.com)
  • An antibody-drug conjugate (enfortumab-vedotin) directed against nectin-4 has shown marked tumor remission rates in this tumor type, which is known for high expression rates of nectin-4. (oncotarget.com)
  • In the past year, four antibody-drug conjugates (ADC) were approved, nearly doubling the marketed ADCs in oncology. (aacrjournals.org)
  • Nathanael Gray is the Krishnan-Shah Family Professor of Chemical and Systems Biology at Stanford, Co-Director of Cancer Drug Discovery Co-Leader of the Cancer Therapeutics Research Program, Member of Chem-H, and Program Leader for Small Molecule Drug Discovery for the Innovative Medicines Accelerator (IMA). (stanford.edu)
  • There, he has established a discovery chemistry group that focuses on developing first-in-class inhibitors for newly emerging biological targets, including resistant alleles of existing targets, as well as inhibitors of well-validated targets, such as Her3 and RAS, that have previously been considered recalcitrant to small molecule drug development. (stanford.edu)
  • Research has begun to explore RNAs potential to be used for therapeutic benefit, and unique challenges have occurred during drug discovery and implementation of RNA therapeutics. (wikipedia.org)
  • Moreover, after antitumor treatment, [ 89 Zr]Zr-DFO-KN035 enabled observational imaging for therapeutic efficacy evaluation, which can help predict patient prognosis. (thno.org)
  • Curcumin can be developed as a therapeutic drug through improvement in formulation properties or delivery systems, enabling its enhanced absorption and cellular uptake. (blogspot.com)
  • Here, we discuss how research has driven cancer drug development in the past and describe how recent advances in biology, technology, our conceptual understanding of cell networks and removal of some roadblocks may facilitate therapeutic advances in the (hopefully) near future. (biomedcentral.com)
  • We employed combinatorial drug screening to identify synergistic combination partners to enhance the efficacy of venetoclax in T-PLL patients. (haematologica.org)
  • Chemotherapeutic drugs target cancer-specific characteristics, such as alterations in DNA repair, which has complicated roles in oncogenesis and tumor progression. (biomedcentral.com)
  • Emerging evidence suggests that they provide a cellular mechanism for induction and emergence of drug resistance and contribute to increased invasive and metastatic potential. (frontiersin.org)
  • Treatment options are limited at best and drug resistance is common. (frontiersin.org)
  • Dr. Gray's research has had broad impact in the areas of kinase inhibitor design and in circumventing drug resistance. (stanford.edu)
  • Dr. Gray has also developed structure-based, generalized approaches for designing drugs to overcome one of the most common mechanisms of resistance observed against most kinase inhibitor drugs, mutation of the so-called 'gatekeeper' residue, which has been observed in resistance to drugs targeting BCR-ABL, c-KIT and PDGFR. (stanford.edu)
  • For cancer, it was considered that the development of resistance to a battery of agents used concurrently, rather than a single drug, was less likely. (biomedcentral.com)
  • Aiming to exploit this effect in therapy we performed a compound screen in five breast cancer cell lines with IGF neutralising antibody xentuzumab. (nature.com)
  • The rationale is compelling for study of SHROOM2 as a gene responsible for susceptibility to a common cancer killer (CRC). (ukri.org)
  • Our pilot studies already suggest SHROOM2 (and related pathway components) is a drug target for developing cancer prevention agents. (ukri.org)
  • Colorectal cancer (CRC) is a common cancer but incidence could be reduced by early detection or prevention with drugs and/or dietary constituents/additives. (ukri.org)
  • In 2021, Dr. Gray joined Stanford University where he has joined the Stanford Cancer Institute, Chem-H and the Innovative Medicines Accelerator (IMA) to spur the development of prototype drugs. (stanford.edu)
  • and many others - see Advanced Drug Delivery Systems of Curcumin for Cancer Chemoprevention for a good overview. (blogspot.com)
  • Several articles have extensively reviewed the history of cancer drug development [ 1 ] and so here we will only pick out the salient points. (biomedcentral.com)
  • It is widely accepted, although clouded by the secrecy of war [ 2 ], that the first tentative steps to treating cancer with drugs emanated from the observation that exposure to chemical warfare agents ('poison gases'), such as nitrogen mustards, could limit the proliferative nature of rapidly dividing lymphoid cells. (biomedcentral.com)
  • This drug, originally developed by Walpole's group at ICI as a potential contraceptive, showed its potential when initially trialed for breast cancer in 1971 [ 8 ]. (biomedcentral.com)
  • Subsequent clinical trials have confirmed the utility of this drug in ERα-positive breast cancer patients and tamoxifen has now been given to millions of women and has saved countless lives. (biomedcentral.com)
  • The interaction between drugs and transport proteins is a key factor in drug bioavailability. (mdpi.com)
  • The rationale for this came from the treatment of tuberculosis, for which antibiotics, each with a different mechanism of action, were more effective when used in combination. (biomedcentral.com)
  • Twenty-four candidate compounds were selected based on their clinical approval status, literature data, and mechanisms of drug action. (haematologica.org)
  • Integration of a machine learning-derived signature of response with in vitro assessment of LP-184 efficacy facilitated the derivation of manageable yet robust biomarkers which can be used to predict drug sensitivity with high accuracy and clinical value. (biomedcentral.com)
  • Drug discontinuation and therapy reconciliation remain the mainstay in patent's management to minimize occurrence of acute liver failure. (wjgnet.com)
  • RESULTS: BRD4 inhibitors JQ1 and ARV-771 were identified as the most promising drugs both in the cisplatin and radiation screening projects in two NSCLC cell lines. (bvsalud.org)
  • 6 In addition, independent annexin V/Hoechst assays of primary T-PLL cells on NK-Tert stromal support demonstrated modest single-agent activity of ibrutinib ( Figure 1D , Online Supplementary Figure S2A ). (haematologica.org)
  • Around the same time, and building on the observation that the vitamin folic acid could stimulate acute lymphoblastic leukemia (ALL) cells, Farber used folate analogs such as aminopterin and then amethopterin (methotrexate) to treat ALL, in what is often heralded as the first 'rational' drug development approach [ 4 ]. (biomedcentral.com)
  • There are a few high-profile examples of rationally and molecularly targeted therapies, but we need to do much better if we are to shift the entire pattern of treatment to drugs that have high potency but mild side effects. (biomedcentral.com)
  • During his six year stay at GNF, Dr. Gray became the director of biological chemistry where he supervised a group of over fifty researchers integrating chemical, biological and pharmacological approaches towards the development of new experimental drugs. (stanford.edu)
  • In this work, we use both approaches to study drug-albumin binding and support the results by comparison with other complementary techniques and by structural and computational analysis. (mdpi.com)
  • The rationale of these traditional uses in African traditional medicine was established by screening several species for biological activities. (scialert.net)
  • We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
  • and discovery that sphingosine-1-phosphate receptor-1 (S1P1) is the pharmacologically relevant target of the immunosuppressant drug Fingomilod (FTY720) followed by the development of Siponimod (BAF312), which is currently used for the treatment of multiple sclerosis. (stanford.edu)
  • Following the discovery of chemotherapeutics, the next significant advance came in the 1960s with the straightforward notion of combining drugs. (biomedcentral.com)
  • Nucleoside (analogue) triphosphate levels were determined by LC-MS/MS. CNDAC interaction with SAMHD1 was analysed by an enzymatic assay and by crystallisation. (biomedcentral.com)
  • Pilot data provide compelling rationale for study of SHROOM2 in CRC initiation and biology. (ukri.org)
  • Our data indicate that the utility of risk-based screening for mpox in persons without skin lesions or rash via pharyngeal swabs, rectal swabs, and/or blood is likely limited. (cdc.gov)
  • There is considerable effort dedicated to finding a way to make curcumin/turmeric more bioavailable, including lipid delivery systems, self-microemulsifying drug delivery system (SMEDDS) , nanoparticulate delivery systems. (blogspot.com)
  • Understanding the causes of CRC would enable the rational development of preventative agents, biomarkers of risk and potentially new chemotherapy drugs. (ukri.org)
  • CNDAC was tested in 13 acute myeloid leukaemia (AML) cell lines, in 26 acute lymphoblastic leukaemia (ALL) cell lines, ten AML sublines adapted to various antileukaemic drugs, 24 single cell-derived clonal AML sublines, and primary leukaemic blasts from 24 AML patients. (biomedcentral.com)
  • The study consists of a maximum 6-week screening period, a 52-week treatment period (including the 26-week tapering), and an 8-week safety follow-up, with primary and secondary endpoint assessments at week 28. (biomedcentral.com)
  • Given that hips with an LCEA less than 20 degrees associated with developing osteoarthritis, we recommended that LCEA between 20 to 25 degrees be used as the radiographic criterion in the primary screening. (bvsalud.org)
  • Drug-induced liver injury (DILI) and herb-induced liver injury is a hot topic for clinicians, academia, drug companies and regulators, as shown by the steadily increasing number of publications in the past 15 years. (wjgnet.com)
  • However, available evidence indicates that CRC incidence can be substantially reduced by early detection and prevention using drugs and/or dietary components. (ukri.org)
  • Cell lines adapted to drugs not affected by DCK or SAMHD1 remained CNDAC sensitive. (biomedcentral.com)