• 22 cultivars of male and female of T. dioica from various agro-climatic regions of India have been fingerprinted by RAPD and ISSR markers utilizing 37, 15 primers respectively. (academicjournals.org)
  • In this activity, 86 loci from 20 RAPD primers were used for analyzing genetic diversity of 10 populations of ramin distributed in Kalimantan and Sumatera. (forda-mof.org)
  • The scientist performing RAPD creates several arbitrary, short primers (10-12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. (wikipedia.org)
  • No knowledge of the DNA sequence of the targeted genome is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. (wikipedia.org)
  • Unlike traditional PCR analysis, RAPD does not require any specific knowledge of the DNA sequence of the target organism: the identical 10-mer primers will or will not amplify a segment of DNA, depending on positions that are complementary to the primers' sequence. (wikipedia.org)
  • A total of 14 RAPD primers of arbitrary sequence were used to amplify pooled genomic DNA from these breeds, and only 10 primers generated reproducible, score able and polymorphic bands. (bu.edu.eg)
  • Genetic distances of F. graminearum and F. culmorum isolates appeared more intense in wheat fields in Adana region were determined by RAPD-PCR (10 primers). (scialert.net)
  • Out of 40 RAPD primers tested, 12 exhibited distinct banding patterns showing 45% to 79% polymorphism. (tubitak.gov.tr)
  • A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. (scielo.br)
  • Foi usado um total de 78 primers RAPD, sendo que 8 apresentaram 54% de fragmentos de DNA polimórficos. (scielo.br)
  • Random Amplified Polymorphic DNA (RAPD) analysis was carried out to study genomic changes using 10 different random primers. (cdc.gov)
  • PCR-based Random Amplified Polymorphic DNA (RAPD) Marker employing 3 decamer primers produced 29 scorable bands of which all (100%) were polymorphic. (banglajol.info)
  • The diversity within and between the two hemp cultivars "Kompolti" and "Fasamo" was examined with 18 RAPD (Random Amplified Polymorphic DNA) primers. (gwdg.de)
  • the use of issr and rapd markers for detecting dna polymorphism, genotype identification and genetic diversity among trichosanthes dioica roxb. (academicjournals.org)
  • This study showed that RAPD and ISSR markers could provide a practical and efficient tool in quality control of the T. dioica . (academicjournals.org)
  • Analysis of differentiation (genetic diversity and related relationships) among 22 landrace ( Ficus carica L. sativa ) and 2 wild form ( F. carica L. caprificus ) accessions of fig growing under the same environmental conditions in the Palestinian Fig Collection, Til, Nablus, Palestine, using PCR-based Random Amplified Polymorphic DNA (RAPD) and pomological markers, revealed considerable genetic diversity. (scirp.org)
  • Clustering patterns obtained from the combined (pomological and RAPD) markers had higher discriminatory power to discriminate fig landraces than using either pomological or RAPD markers alone. (scirp.org)
  • These results proved the importance of both pomological and RAPD markers to elucidate in part denomination problems and relationships among cultivars. (scirp.org)
  • Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants. (forda-mof.org)
  • RAPD markers are decamer (10 nucleotides long) DNA fragments from PCR amplification of random segments of genomic DNA with a single primer of arbitrary nucleotide sequence and which are able to differentiate between genetically distinct individuals, although not necessarily in a reproducible way. (wikipedia.org)
  • Nearly all RAPD markers are dominant, i.e. it is not possible to distinguish whether a DNA segment is amplified from a locus that is heterozygous (1 copy) or homozygous (2 copies). (wikipedia.org)
  • Codominant RAPD markers, observed as different-sized DNA segments amplified from the same locus, are detected only rarely. (wikipedia.org)
  • Random Amplified Polymorphic DNA (RAPD) markers were employed to assess the genetic diversity and phylogenetic relationships among three Egyptian rabbit breeds (Gabali, Baladi Red and Baladi Black) along with New Zealand White breed as a reference population. (bu.edu.eg)
  • Six Random Amplified Polymorphic DNA (RAPD) markers linked to high sugars were found, five dominant and one co-dominant. (usda.gov)
  • Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) dominant markers were used to assess genetic variability in the aphid Sitobion avenae (Fabricius) in relation to hydroxamic acid levels in their host-plants. (cambridge.org)
  • The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. (scielo.br)
  • This field of genetics emerged from the availability of several studies with classical molecular markers, such as isozymes, RAPD, AFLP, and microsatellites. (intechopen.com)
  • The genetic diversity of 10 diploid and tetraploid wheat species was estimated using random amplified polymorphic DNA (RAPD) markers. (tubitak.gov.tr)
  • The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. (bvsalud.org)
  • Results: Characterization using RAPD markers uncovered significant genetic variability among the samples and indicated the existence of clusters, which allowed us to reconstruct both the origin and clinical history of the disease. (bvsalud.org)
  • RAPD (Random Amplified Polymorphic DNA) markers have been most widely used for this purpose 8-10 . (bvsalud.org)
  • The dominance of L. brevis CRL 2051 and F. tropaeoli CRL 2034 was confirmed by randomly amplified polymorphic DNA (RAPD)-PCR analysis. (unipa.it)
  • Isolates (50) of E. coli obtained from liquid manure (20 bovine, 20 porcine) were genotyped using random amplified polymorphic DNA (RAPD). (iwaponline.com)
  • All isolates from soil and only one from groundwater showed the RAPD pattern of the spread E. coli strain. (iwaponline.com)
  • To investigate the molecular epidemiology of this outbreak, we produced DNA fingerprints of MG isolates by random amplification of polymorphic DNA (RAPD). (cdc.gov)
  • All MG isolates from songbirds had RAPD banding patterns identical to each other but different from other strains and isolates tested. (cdc.gov)
  • The RAPD-PCR analysis showed that F. graminearum and F. culmorum isolates collected from Adana/Turkey were genetically varied. (scialert.net)
  • Random Amplified Polymorphic DNA (RAPD)-PCR was used for a phylogenetic study to check the homology of pairs of isolates. (biomedcentral.com)
  • While the homology of the isolates was low among different patients during hospitalization, identical (9%) and similar (21%) RAPD-PCR patterns were detected between pairs of isolates from each patient. (biomedcentral.com)
  • Genetic diversity between bioluminescent isolates was assessed with two PCR-based DNA fingerprinting methods, i.e. (bvsalud.org)
  • Genomic DNA was extracted and PCR amplification was performed and the amplification products were resolved by electrophoresis. (bu.edu.eg)
  • Further genotypic comparison was performed using different genomic DNA fingerprint methods including BOX-PCR, (GTG) 5 -PCR, and three RAPD-PCRs. (springer.com)
  • The sequence analysis of the genes gap and tuf and the genomic DNA fingerprinting results revealed, as noval findings, that the six T. abortisuis strains cultured from a single farm represent six different bacterial clones showing a genetic variability of this bacterial species in the pig population. (springer.com)
  • No significant genomic changes were observed by RAPD analysis. (cdc.gov)
  • The percentage of polymorphism range for RAPD is from 89 to 45% while for ISSR is from 88 to 100%, the UPGMA dendogram obtained from the cluster analysis of RAPD and ISSR data gave similar clustering pattern, with Jaccards similarity coefficient ranging from 0.23 to 0.93. (academicjournals.org)
  • RAPD, ISSR, genetic diversity. (academicjournals.org)
  • RAPD requires only one primer for amplification. (wikipedia.org)
  • Taq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer that minimizes the need for optimization of PCR parameters, as well as Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g. (qiagen.com)
  • However, all of these methods involve PCR amplification of target DNA that is typically more than hundred base-pair long [ 25 - 30 ]. (hindawi.com)
  • RXL10 F2 mapping population, was enriched with 179 AFLP and 19 RAPD marker loci. (edu.pl)
  • AFLPs generated by EcoRI/MseI primer combinations were distributed over the entire genome as distinct loci or clusters of 2-14 tightly linked DNA fragments. (edu.pl)
  • A detailed description of the newly mapped AFLP and RAPD loci is presented. (edu.pl)
  • Khadari, B., Lashermes, P.H. and Kjellberg, F. (1995) RAPD Fingerprints for Identification and Genetic Characterization of Fig (Ficus carica L.) Genotypes. (scirp.org)
  • The distinct strain patterns found in different kinds of liquid manure suggest that genotyping of E. coli by RAPD may be an adequate tool for tracing sources of faecal contamination. (iwaponline.com)
  • By resolving the resulting patterns, a semi-unique profile can be gleaned from an RAPD reaction. (wikipedia.org)
  • The RAPD marker (OPA04) revealed variations in germinated maize seeds after treatment with different duration of low temperatures. (mendeley.com)
  • The RAPD-PCR phenotypic pattern showed the appearance of two new RAPD-PCR variants after four to five generations of exposure to wheat cv. (cambridge.org)
  • While the overall phenotypic 'richness' diminished during the season, the number of RAPD-PCR phenotypes decreased on cv. (cambridge.org)
  • Because it relies on a large, intact DNA template sequence, it has some limitations in the use of degraded DNA samples. (wikipedia.org)
  • Genome sequence of Sclerotinia sclerotiorum hypovirulence-associated DNA virus-1 found in the fungus Penicillium olsonii isolated from Washington state, USA. (usda.gov)
  • The biosensor probe identified a target DNA sequence that was several-folds shorter than a typical PCR-template. (hindawi.com)
  • Thus, the RAPD technique is notoriously laboratory dependent and needs carefully developed laboratory protocols to be reproducible. (wikipedia.org)
  • It is an unenveloped, non-covalently closed, bacilliform double-stranded DNA (dsDNA) virus with a monopartite genome of ~7.2-7.8 kb encoding three open-reading frames (ORFs). (nature.com)
  • Mutagenesis of laser radiation on DNA and agronomic traits in chickpea (Cicer arietinum L. (ac.ir)
  • Thus, the RAPD results can be difficult to interpret, unlike traditional PCR analysis. (wikipedia.org)
  • Comparative RAPD analysis and pollen structure studies of Bellis peren" by NESLİHAN KAVALCIOĞLU, LEYLA AÇIK et al. (tubitak.gov.tr)
  • Comparative RAPD analysis and pollen structure studies of Bellis perennis L. (tubitak.gov.tr)
  • Random amplified polymorphic DNA (RAPD) is one of the easiest and most commonly used molecular techniques for genetic variability analysis. (tubitak.gov.tr)
  • In the present study, the genetic diversity of analysis of Bellis perennis L. collected in 4 Turkish geographical locations was investigated by RAPD-PCR analysis. (tubitak.gov.tr)
  • In addition to RAPD, pollen analysis of the 4 plant populations was also conducted. (tubitak.gov.tr)
  • New gene-based technologies can now be employed to examine microbial diversity through the use of small sub-unit ribosomal DNA analysis (e.g. 16S rDNA) and to understand the function of complex microbial ecosystems in the rumen through metagenomic analysis. (nhbs.com)
  • Moreover, the recent report on frequent artifacts on real-time PCR results [ 33 ] has raised the questions of validity of the existing DNA-based methods in the analysis of highly processed meat products. (hindawi.com)
  • The Taq DNA Polymerase is intended for molecular biology applications. (qiagen.com)
  • A caracterização molecular foi baseada em marcadores RAPD, sequenciamento da região do espaçador interno transcrito do DNA ribossomal e "fingerprinting" telomérico. (scielo.br)
  • Kurz] dari Provinsi Riau Berdasarkan Profil Random Amplified Polymorphic DNA. (forda-mof.org)
  • Random amplified polymorphic DNA (RAPD), pronounced "rapid", is a type of polymerase chain reaction (PCR), but the segments of DNA that are amplified are random. (wikipedia.org)
  • The aim of the current study was to estimate the genetic diversity of F. graminearum and F. culmorum isolated from wheat in Adana province of Turkey by investigating by RAPD-PCR polymorphisms. (scialert.net)
  • Differential appearance of new RAPD-PCR aphid phenotypes also occurred on field-grown wheat. (cambridge.org)
  • PCR is an enzymatic reaction, therefore, the quality and concentration of template DNA, concentrations of PCR components, and the PCR cycling conditions may greatly influence the outcome. (wikipedia.org)
  • DNA was extracted and this was followed by RAPD-polymerase chain reaction. (mendeley.com)
  • Decreased pupillary light reaction in the affected eye: A relative afferent pupillary defect (RAPD) or Marcus Gunn pupil commonly is observed. (medscape.com)
  • RAPD) rabbit antiserum provided by S.H. (cdc.gov)
  • Several studies implicated that longer DNA targets are broken down to small fragments during extensive food processing causing failure of PCR-based identification methods [ 23 , 26 , 28 , 31 ]. (hindawi.com)
  • Considerable differences were observed in the RAPD profile and pollen shape of plants from the Rize/Dereköy location. (tubitak.gov.tr)
  • The present report is, therefore, a step to protect the plant breeders rights by making use of reliable and modern DNA technologies. (academicjournals.org)
  • In recent years, RAPD has been used to characterize, and trace, the phylogeny of diverse plant and animal species. (wikipedia.org)
  • This makes the method popular for comparing the DNA of biological systems that have not had the attention of the scientific community, or in a system in which relatively few DNA sequences are compared (it is not suitable for forming a cDNA databank). (wikipedia.org)
  • The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences to address a wide ranging biological problem in food industry, diagnostic laboratories and forensic medicine. (hindawi.com)
  • Therefore, if a mutation has occurred in the template DNA at the site that was previously complementary to the primer, a PCR product will not be produced, resulting in a different pattern of amplified DNA segments on the gel. (wikipedia.org)
  • Für die Diversität innerhalb und zwischen den beiden Sorten "Kompolti" und "Fasamo" wurden 18 RAPD-Primer benutzt. (gwdg.de)
  • Its resolving power is much lower than targeted, species-specific DNA comparison methods, such as short tandem repeats. (wikipedia.org)
  • 2000) suggest that the heredtary component s as the role of reactve oxygen speces (ROS) n DNA greater. (who.int)
  • One porcine strain, showing a simple RAPD pattern, was subcultured and spread on a test field (1.5l/m 2 at 10 10 cfu/l) in a drinking water protection zone with loamy to sandy sediments in the Donauried area, Baden-Wurttemberg. (iwaponline.com)
  • Other taxonomic data: RAPD PCR [ 6808 ]. (riken.jp)