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Aminonucleoside antibioticAntibiotic selectionInhibitionAminopeptidaseResistanceStreptomycesGeneSelectionColiMRNAMiceCellsHumanSolutionAntibioticInhibits protein synthesisFluorescent puromycinInactivates puromycinSoluble in waterPromoterMarkerShRNAMammalian cells2023LentiviralProteinAntibioticsConcentrationsSelective agentMolecularExpressionGrowing peptideOptimumDaysSelection requiresLevelNoteCell linesEffectiveLinkMedium
Aminonucleoside antibiotic2
  • Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. (wikipedia.org)
  • The biosynthetic pathway of the aminonucleoside antibiotic puromycin, as deduced from the molecular analysis of the pur cluster of Streptomyces alboniger. (kegg.jp)
Antibiotic selection1
  • This lentivirus also contain the Puromycin selection marker under the constitutive RSV promoter for antibiotic selection. (gentarget.com)
Inhibition2
  • The mechanism of inhibition is not well understood, however puromycin can be used to distinguish between aminopeptidase M (active) and cytosol alanyl aminopeptidase (inhibited by puromycin). (wikipedia.org)
  • Puromycin shows appreciable inhibition against the mammary adenocarcinoma of the C3H mouse, the mammary adenocarcinoma 755 of the C57BL mouse, and a glioblastoma cultivated in the chick embryo. (jamanetwork.com)
Aminopeptidase2
  • Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase (metallopeptidase). (wikipedia.org)
  • Specific puromycin-sensitive aminopeptidase (PSA) inhibitors with a 3-(halogenated phenyl)-2,4(1H,3H)-quinazolinedione skeleton were prepared and their structure-activity relationships were investigated. (elsevierpure.com)
Resistance3
  • Resistance to puromycin is conferred by the pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain. (wikipedia.org)
  • Plates containing puromycin are stable for 1 month when stored at 4 °C.[citation needed] Puromycin resistance in yeast can also be conferred through expression of the puromycin N-acetyl-transferase (pac) gene. (wikipedia.org)
  • Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. (addgene.org)
Streptomyces1
Gene1
  • Deletion of the gene encoding the multidrug efflux pump Pdr5 sensitizes cells to puromycin. (wikipedia.org)
Selection3
  • Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures is within a range of 1-10 μg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/ml. (wikipedia.org)
  • But use of puromycin for E. coli selection requires precise pH adjustment and also depends on which strain is selected. (wikipedia.org)
  • For hassle-free selection and optimum results the use of specially modified puromycin is possible. (wikipedia.org)
Coli1
  • citation needed] Puromycin is poorly active on E. coli. (wikipedia.org)
MRNA3
  • Also of note, puromycin is critical in mRNA display. (wikipedia.org)
  • In this reaction, a puromycin molecule is chemically attached to the end of an mRNA template, which is then translated into protein. (wikipedia.org)
  • The puromycin can then form a covalent link to the growing peptide chain allowing the mRNA to be physically linked to its translational product. (wikipedia.org)
Mice2
  • As puromycin inhibits protein synthesis in eukaryotic cells, researchers were able to show that injections of this drug will result in both short-term as well as long-term memory loss in mice. (wikipedia.org)
  • Toxicity studies in mice indicate the LD 50 values of puromycin to be 360 mg/kg. (jamanetwork.com)
Cells1
  • Puromycin acts quickly and can kill up to 99% of nonresistant cells within 2 days. (wikipedia.org)
Human1
  • WRIGHT JC , DOLGOPOL VB , LOGAN M , PRIGOT A , WRIGHT LT. Clinical Evaluation of Puromycin in Human neoplastic Disease. (jamanetwork.com)
Solution1
  • Puromycin is soluble in water (50 mg/ml) as colorless solution at 10 mg/ml. (wikipedia.org)
Antibiotic9
  • Puromycin is an antibiotic protein synthesis inhibitor which causes premature chain termination during translation. (wikipedia.org)
  • Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. (wikipedia.org)
  • Puromycin dihydrochloride is a aminonuclease antibiotic that inhibits protein synthesis. (openwetware.org)
  • Prior to using the puromycin antibiotic, titrate the selection agent to determine the optimal concentration for target cell line. (openwetware.org)
  • The minimum antibiotic concentration to use is the lowest concentration that kills 100% of the cells in 3-5 days from the start of puromycin selection. (openwetware.org)
  • Puromycin is an aminonucleoside antibiotic that causes early chain termination within the ribosome during translation. (goldbio.com)
  • Puromycin Hydrochloride Solution is the hydrochloride salt form of puromycin, an aminonuclease antibiotic agent isolated from the bacterium Streptomyces alboniger. (canvaxbiotech.com)
  • The RPM is based on ribosome-catalyzed puromycylation of nascent chains immobilized on ribosomes by antibiotic chain elongation inhibitors followed by detection of puromycylated ribosome-bound nascent chains with a puromycin (PMY)-specific monoclonal antibody in fixed and permeabilized cells. (nih.gov)
  • Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. (sigmaaldrich.com)
Inhibits protein synthesis1
  • As puromycin inhibits protein synthesis in eukaryotic cells, researchers were able to show that injections of this drug will result in both short-term as well as long-term memory loss in mice. (wikipedia.org)
Fluorescent puromycin1
  • Here, we demonstrated the C-terminus-specific fluorescence labeling of histidine-tagged thrombopoietin (TPO), a ligand for Mpl, with desthiobiotin-tagged fluorescent puromycin. (elsevierpure.com)
Inactivates puromycin1
  • Its gene product is puromycin-N-acetyl-transferase which inactivates puromycin through acetylation. (goldbio.com)
Soluble in water2
  • Puromycin is soluble in water (50 mg/ml) as colorless solution at 10 mg/ml. (wikipedia.org)
  • Puromycin is soluble in water and is often used as a selective agent in molecular biology research. (goldbio.com)
Promoter3
  • Lentiviral vector-human synapsin 1 promoter, Puro contains Multiple Cloning Sites (MCS) downstream of the human synapsin 1 promoter, and a puromycin selection marker driven by the PGK promoter. (cellomicstech.com)
  • GenTargetDescription:Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with puromycin marker. (reportergene.com)
  • GenTargetDescription:Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Puromycin marker, provided in DMEM medium with 10% FBS.Storage conditions: (-80°C) Shipping. (reportergene.com)
Marker2
ShRNA2
  • 48 hours post- shRNA transfection , aspirate the medium and replace with fresh medium containing puromycin at the appropriate concentration. (openwetware.org)
  • Tumor necrosis factor receptor-associated factor 6 (TRAF6) plays a role in diffuse large B cell lymphoma (DLBCL) biological processes.TRAF6 Stable Knockdown HT Cell Line was generated via lentivirus transduction with TRAF6 shRNA plasmids, and the transfectant was selected with puromycin. (topsan.org)
Mammalian cells1
  • The growth of bacteria, protozoa, algae and mammalian cells is quickly halted by puromycin. (goldbio.com)
20232
Lentiviral1
  • T6411HEK293T cells were tranfected with pGIPZ lentiviral shRNAs targeting PC4, and were selected with 3 µg/ml puromycin to establish the PC4 Knockdown. (topsan.org)
Protein4
  • In this reaction, a puromycin molecule is chemically attached to the end of an mRNA template, which is then translated into protein. (wikipedia.org)
  • Acting as an analog of the 3′ terminal end of aminoacyl-tRNA, puromycin incorporates itself into a growing polypeptide chain and causes its premature termination, thereby inhibiting protein synthesis. (canvaxbiotech.com)
  • Puromycin labeling does not allow protein synthesis to be measured in energy-starved cells. (nih.gov)
  • 6. Effect of a low-protein diet on expression of non-muscle type myosin heavy-chain isoforms in glomeruli of rats with puromycin aminonucleoside nephrosis. (nih.gov)
Antibiotics1
  • Lot-to-lot variations in potency exist for all selection antibiotics, each new lot of puromycin should be titrated. (openwetware.org)
Concentrations2
  • Puromycin is stable for one year as solution when stored at -20 °C. The recommended dose as a selection agent in cell cultures is within a range of 1-10 μg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/ml. (wikipedia.org)
  • Lethal concentrations of puromycin are much higher for strains of Saccharomyces cerevisiae than mammalian cell lines. (wikipedia.org)
Selective agent1
  • Puromycin is used in cell biology as a selective agent in cell culture systems. (wikipedia.org)
Molecular1
Expression2
  • 7. Altered expression of NDST-1 messenger RNA in puromycin aminonucleoside nephrosis. (nih.gov)
  • 13. Impact of cyclosporin on podocyte ZO-1 expression in puromycin aminonucleoside nephrosis rats. (nih.gov)
Growing peptide1
  • The puromycin can then form a covalent link to the growing peptide chain allowing the mRNA to be physically linked to its translational product. (wikipedia.org)
Optimum1
  • For hassle-free selection and optimum results the use of specially modified puromycin is possible. (wikipedia.org)
Days1
  • The cell populations were selected with puromycin and then differentiated for 7 days to form myotubes. (nih.gov)
Selection requires1
Level1
  • Following puromycin removal, low-level latent infection was maintained. (nih.gov)
Note1
  • Also of note, puromycin is critical in mRNA display. (wikipedia.org)
Cell lines1
  • The working puromycin concentration for mammalian cell lines ranges from 1-10 µg/ml. (openwetware.org)
Effective2
  • Research has shown puromycin to be effective against certain tumors. (goldbio.com)
  • Iodoquinol, puromycin, and nitazoxanide are also effective against balantidiasis. (medscape.com)
Link2
  • Link to all annotated objects annotated to puromycin metabolic process. (planteome.org)
  • Link to all direct and indirect annotations to puromycin metabolic process. (planteome.org)
Medium1
  • Puromycin-resistant transformants are selected in LB agar medium supplemented with 125 µg/ml of puromycin. (wikipedia.org)