Proteins immunization secondary. Medical search. Frequent questions

These data suggest that the addition of a late protein boost alone is sufficient to increase functionally potent vaccine-specific antibodies previously associated with reduced risk of infection with HIV. (nih.gov)
Epstein-Barr virus is also known to induce dsDNA antibodies, as seen after immunisation of animals with EBNA-1 epitopes. (wikipedia.org)
Anti-dsDNA antibodies might also be created secondary to the production of antibodies to other proteins within the nucleosome. (wikipedia.org)
The spike (S) protein found on the surface of the causative agent, the SARS-CoV-2 virus, has been the prime target for current vaccine research since antibodies directed against the S protein were found to neutralize the virus. (mdpi.com)
Passive immunization means the provision of temporary immunity by the administration of preformed antibodies. (cdc.gov)
In children who have completed immunizations with diphtheria, pertussis, and tetanus (DPT) or Hib-conjugated vaccines, the antibody response to protein antigens can be tested in adults and older children by measuring IgG antibodies to tetanus or diphtheria toxoid and H influenzae type b (Hib) polysaccharide antigen. (medscape.com)
Approximately 80% of children have detectable antibodies to these antigens after 3 immunizations during their first year of life. (medscape.com)
Obtain blood 4 weeks after the last immunization and determine IgG antibodies. (medscape.com)
Secondary vac- many were cases of primary infection (i.e. cine failures, however, are largely attributed low-avidity antibodies). (who.int)
The problem of obtaining from precipitation experiments evidence about the structure of antibodies and the nature of serological reactions is obviously greatly simplified by the replacement of protein antigens by simple substances of known structure. (caltech.edu)
Some success has been obtained also in the formation of antibodies from other serum proteins and by other denaturation-renaturation procedures. (caltech.edu)
By means of immunohistochemistry and double-labeling studies using transgenic mouse lines as well as Western blot analyses, it was demonstrated that the newly generated antibodies specifically recognized the receptor proteins. (jneurosci.org)
To scrutinize the hypothesis that olfactory receptor proteins may also be present in the axonal processes and the nerve terminals, serial sections through the olfactory bulb were probed with the antibodies. (jneurosci.org)
In this study, antibodies were generated against unique epitopes of distinct OR types and used in immunohistochemical experiments to visualize the receptor proteins in whole-mount preparations and tissue sections of the olfactory system. (jneurosci.org)
The overall objective of EuroNeut-41 was to design a vaccine that raises antibodies able to prevent HIV cell fusion by blocking the virus fusion protein gp41 in its pre-fusogenic conformation. (europa.eu)
The mouse model expressed a fluorescent protein, TdTomato, in cells that produced antibodies against a specific vaccine. (scienceboard.net)
Secondary Antibodies are available in a variety of formats and conjugate types. (rockland.com)

Intended for intramuscular administration, IG is primarily indicated for routine maintenance of immunity of certain immunodeficient persons and for passive immunization against measles and hepatitis A. IG does not transmit hepatitis B virus, human immunodeficiency virus (HIV), or other infectious diseases. (cdc.gov)
Immunization is a more inclusive term denoting the process of inducing or providing immunity artificially by administering an immunobiologic. (cdc.gov)
Although persons often use vaccination and immunization interchangeably in reference to active immunization, the terms are not synonomous because the administration of an immunobiologic cannot be automatically equated with the development of adequate immunity. (cdc.gov)
Vaccination of mice using the West Nile virus E-protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects mice against a lethal challenge. (sciensano.be)
Screening the array with sera and ileal fluid samples from immunized pigs suggested cross-reactivity among homologous proteins and a general activation of immunity. (frontiersin.org)
Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. (frontiersin.org)

Active immunization is the production of antibody or other immune responses through the administration of a vaccine or toxoid. (cdc.gov)
This assessment can also be done by measuring antibody responses following active immunization with protein or polysaccharide antigens. (medscape.com)

Upon release of nuclear proteins and chromatin, antigen presenting cells, such as dendritic cells and macrophages, display these antigens to T helper cells. (wikipedia.org)
Assessment of responses to polysaccharide antigens is important in patients older than 18-24 months because these responses may be deficient in some patients who can respond normally to protein antigens. (medscape.com)
Therefore, antibody responses should be measured to polysaccharide antigens that are not present in the protein-conjugated pneumococcal vaccine. (medscape.com)
Exposure to the measles virus is steadily been known to distinguish primary from decreasing worldwide due to mass vaccina- secondary immune responses against many tion, and millions of people are protected antigens. (who.int)
Attempts were made to confer insolubility on protein antigens such as ovalbumin and crystalline bovine serum albumin without destroying their antigenicity. (caltech.edu)
These findings provide six novel immunogenic Shigella proteins that could serve as candidate vaccine antigens, species-specific carrier proteins, or targeted adjuvants. (biomedcentral.com)

The RV144 HIV-1 vaccine trial results showed moderate reduction in viral infections among vaccinees as well as induction of antibody-dependent cellular cytotoxicity and vaccine-specific IgG and IgG3 responses directed at variable loop regions 1 and 2 of the HIV envelope protein. (nih.gov)
The companion vaccine trial RV305 was designed to permit the evaluation of the immunologic impact of late boosting with either the boosting protein antigen alone, the canarypox viral vector ALVAC alone, or a combination of both. (nih.gov)
Thus, here we analyzed the antibody and functional profile induced by RV305 boosting regimens and found that although IgG1 levels increased in both arms that included protein boosting, IgG3 levels were reduced compared with the original RV144 vaccine strategy. (nih.gov)
5' noncoding region] and significantly lower neurovirulence supplementary immunization activity (SIA) to sample collection of fecally shed vaccine virus in transgenic mice) ( 3 - 5 ). (cdc.gov)
Protein conjugated pneumococcal vaccine elicit antibody responses that are T-cell dependent. (medscape.com)
This document, the "Advisory Committee Statement: Canadian Immunization Guide Chapter on Influenza and National Advisory Committee on Immunization (NACI) Statement on Seasonal Influenza Vaccine for 2020-2021", updates NACI's recommendations regarding the use of seasonal influenza vaccines. (canada.ca)
ABSTRACT In this study in the Islamic Republic of Iran 365 measles cases were evaluated to distin- guish between primary infection with measles and reinfection due to secondary vaccine failure. (who.int)
Of 244 patients with documented vaccination, 75.8% showed a primary immune response and 24.2% showed a secondary immune response, thereby indicating a secondary vaccine failure. (who.int)
Thus, avidity ures, primary failure and secondary failure, measurement can be used to assess the suc- are attributed to measles vaccine. (who.int)
Primary cess of measles vaccination [ 16 ] and offers failure indicates that the vaccine has not a way of assessing the type of vaccine taken and does not induce any immuno- failure without knowledge of prior antibody logical response, while secondary failure status [ 15,17,18 ]. (who.int)
ABSTRACT Haemophilus influenzae type b (Hib) vaccine was included in the Yemen immunization programme in 2005. (who.int)

Serum amyloid P component is a protein that is thought to aid in the clearance of chromatin produced by apoptotic cells and deficiencies in this protein have been shown (in mice) to cause spontaneous formation of ANA. (wikipedia.org)
We found that the MCEPH protein is expressed in the brain of mceph/mceph mice. (biomedcentral.com)
We divided mice into three groups: human recombinant centromere protein-A (human CENP-A, HA) and complete Freund's adjuvant (CFA) were used to immunize mice for the study group (HA + CFA), and mice injected with CFA (CFA group) or saline (Saline group), respectively, served as controls. (biomedcentral.com)
was subsequently clearly documented by means of genetically manipulated mice that generated a bicistronic mRNA encoding the OR as well as a marker protein. (jneurosci.org)

[ 6 ] In humans, about 23 different unrelated proteins are known to form amyloid fibrils in vivo. (medscape.com)

Recent observations raise the hypothesis that not only the drug/chemical, but also parts of the haptenated protein or peptides may constitute the important structural determinants for antigen recognition by the TCR. (frontiersin.org)
In a recent report by Garvey and Campbell (2) the loss of antigen material from hepatic tissue following a secondary injection of the native protein carrier was clearly demonstrated by radioautography. (caltech.edu)
Suppression was dependent on the ratio of haptenic groups on the molecule, appeared to be greatly affected by the method of immunization, and could be demonstrated in all three antigen systems. (caltech.edu)
The retention of antigen in rabbit liver tissue, resulting from a primary intravenous injection, is influenced by immunization brought about by subsequent intravenous injections of the same antigen. (caltech.edu)
In rabbits given a single primary intravenous injection of radioactive antigen, the retention of radioactivity in liver tissue, after a period of 21 days, was greater than when the primary injection was followed by secondary injections of the same, but non-radioactive antigen. (caltech.edu)
Passively administered homologous antibody did not produce a change in the retention of the primary injection of antigen nor did secondary injections of a heterologous native protein injected according to the same immunization schedule as the homologous azoprotein. (caltech.edu)
1) The general approach has been to attempt to produce an insoluble protein antigen which would combine specifically with antibody to give a complex that could be dissociated into soluble antibody and insoluble antigen, which could then be separated by centrifugation. (caltech.edu)
However, in every case the resulting product either had lost too much native antigenicity (i.e., adsorbed serum always contained antibody which reacted with native antigen), was too soluble, or had too much power of adsorption for non-specific protein. (caltech.edu)
Persistent plasma cells accrue in bone marrow "at an approximately constant rate" of one cell per hour for several weeks after a single immunization with a model antigen. (scienceboard.net)

A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)

Polymorphisms that slightly vary native peptides or inflammatory processes set the stage for abnormal protein folding and amyloid fibril deposition. (medscape.com)
Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)

B7 homologous protein (B7h)/B7-related protein 1 (B7RP-1) is a new member of the B7 family of costimulatory molecules that specifically interacts with inducible costimulator (ICOS) expressed on activated T cells. (aai.org)
An antibody-protein array of putative immunogenic proteins was developed from a combined bioinformatic, experimental, and literature-based prioritization of homologous parasite proteins. (frontiersin.org)

The protective efficacy of various immunization regimes correlated with the magnitude of induced immune responses, supporting the strategy of maximizing durable T cell immunogenicity to develop more effective liver-stage vaccines against Plasmodium falciparum malaria. (ox.ac.uk)

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab) or Bovine, Horse or Human Serum Proteins. (rockland.com)

RÉSUMÉ Dans cette étude en République islamique d'Iran, 365 cas de rougeole ont été évalués pour distinguer une primo-infection rougeoleuse de la réinfection due à l'échec de la vaccination secondaire. (who.int)
RÉSUMÉ Le vaccin contre Haemophilus influenzae type b (Hib) a été inclus dans le programme de vaccination du Yémen en 2005. (who.int)

Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)

We have recently conducted a first-in-human clinical trial to evaluate the safety and immunogenicity of the recombinant, full-length merozoite surface protein 1 (MSP1 FL ) formulated with GLA-SE as adjuvant. (nature.com)
Freund's complete adjuvant is used in the first step of the immunization procedure. (fishersci.com)
The hematopoietic cells are not activated and respond to immunization by foreign Ag and adjuvant. (cnrs.fr)

Here, we developed a double antibody sandwich quantitative enzyme-linked immunosorbent assay (DAS-ELISA) for detection of PDCoV by using a specific monoclonal antibody against the PDCoV N protein and an anti-PDCoV rabbit polyclonal antibody . (chxa.com)
The purified protein was used for immunization of a rabbit to produce specific polyclonal antiserum. (chxa.com)

Immunization history was complete till age as per the expanded programme of immunization (EPI) schedule. (pediatriconcall.com)

Using a cell apoptosis assay based on stimulation of a RANK:Fas fusion protein, and a cellular NF-kappaB signaling assay, we showed that R12-31 was agonist for both species. (cnrs.fr)

The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
F(ab')2 Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey. (rockland.com)
All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)

The Secondary Endpoint, which is efficacy will be evaluated at baseline and days 30, 60, and 90. (therapeuticsolutionsint.com)

When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. (rockland.com)

ICOSL is a 40 kDa immune checkpoint protein belonging to the Ig receptor superfamily. (bioxcell.com)
This concept implies that olfactory receptor proteins are located in sensory cilia and in the axons. (jneurosci.org)
These data indicate that olfactory receptor (OR) proteins are indeed present in the axonal processes and nerve terminals of olfactory sensory neurons, thus supporting the notion that ORs may participate in the molecular processes underlying the fasciculation and targeting of olfactory axons. (jneurosci.org)

The mature B cells circulate between secondary lymphoid organs and migrate into lymphoid follicles of the spleen and lymph nodes in response to further stimuli and various chemokines. (medscape.com)
Reticular fibroblastic and vascular stromal cells, important for secondary lymphoid organ formation and organization, express RANK and undergo hyperproliferation, which is abrogated by RANKL neutralization. (cnrs.fr)
Earlier studies showed that LLPCs originate in germinal centers, specialized microstructures found in secondary lymphoid tissues, and relocate to bone marrow. (scienceboard.net)

Nigéria, en décembre 2022 et publie depuis lors des rapports mensuels. (who.int)

This control addresses whether tissue elements are inadvertently binding immunoglobulin from the same species as the primary antibody, in addition to non-specific binding from the secondary detection reagents. (vectorlabs.com)
Note that the use of pre-immune immunoglobulin, obtained prior to immunization, could also be used. (vectorlabs.com)
1) The reason we are reporting this case is due to a rare presentation of WAS where Wiskott Aldrich Syndrome protein (WASp) deficiency was documented but his immunoglobulin levels were within normal limits and instead of the characteristic feature of microthrombocytopenia the patient presented with macrothrombocytopenia. (pediatriconcall.com)

in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)

The viral genome encodes four structural capsid proteins (VP1 to VP4) and seven nonstructural (NS) proteins, the leader Lb/ab protease, and proteins encoded in the P2 (2B and 2C) and P3 (3A, 3B, 3C, and 3D) regions ( 9 ). (asm.org)
2) The abnormal gene is located on the short arm of the X chromosome at Xp11.22-p11.23 near the centromere and encodes the WAS protein (WASp), which is a 501-amino acid cytoplasmic protein that manifests in lymphocyte and megakaryocyte cell lines. (pediatriconcall.com)

Amyloid fibrils are protein polymers comprising identical monomer units (homopolymers). (medscape.com)
All types of amyloid consist of one major fibrillar protein that defines the type of amyloid. (medscape.com)
[ 5 ] Only 10% of amyloidosis deposits consist of components such as glycosaminoglycans (GAGs), apolipoprotein-E (apoE), and serum amyloid P-component (SAP), while nearly 90% of the deposits consist of amyloid fibrils that are formed by the aggregation of misfolded proteins. (medscape.com)
The amyloidoses are referred to with a capital A (for amyloid) followed by an abbreviation for the fibril protein. (medscape.com)

A molecular model of the FMDV 3A protein, derived from the nuclear magnetic resonance (NMR) structure of the poliovirus 3A protein, predicted a hydrophobic interface spanning residues 25 to 44 as the main determinant for 3A dimerization. (asm.org)

Most functional responses increased upon protein boosting, regardless of the viral vector-priming agent incorporation. (nih.gov)
NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)

More recently, we found that embryos cultured with anti-CENP-A (centromere protein-A) antibody had significant growth impairment and/or high mortality [ 12 ]. (biomedcentral.com)

This compendium of immunization recommendations for adults (≥18 years) with HIV was compiled by the New York State Department of Health AIDS Institute (NYSDOH AI) to assist clinical practitioners in New York State who provide primary care to adults with HIV. (hivguidelines.org)

There is a higher rate of apoptosis in people with SLE and various changes in genes and proteins have been implicated in the defects in apoptosis. (wikipedia.org)

Three types of immunobiologics are administered for passive immunization: a) pooled human IG or IGIV, b) specific immune globulin preparations, and c) antitoxins. (cdc.gov)
It does not react with IgG, IgM and IgG/Fab or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. (fishersci.com)
The Ribosomal protein L13a qPCR control kit contains enough reagents for up to 250 - 50 µl reactions using Human cDNA. (eurogentec.com)

These proteins either arise from proteins expressed by cells at the deposition site (localized), or they precipitate systemically after production at a local site (systemic). (medscape.com)
The bacterial cells expressed recombinant TSWV-N protein up to a concentration of 9.48 µg/ml. (chxa.com)
When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)
These spherical proteins are the keys the virus uses to get in and out of cells in its host organism respectively and to which the Hs and Ns in flu virus names correspond, as in H1N1. (asbmb.org)
In individuals with Waldenström's macroglobulinemia, abnormal plasma cells produce excessive amounts of a protein called macroglobulin, which can lead to thickening of the blood and impair the normal functioning of various organs. (imedix.com)

Amyloidosis results from the accumulation of pathogenic amyloids-most of which are aggregates of misfolded proteins-in a variety of tissues. (medscape.com)

Most classification systems included primary (ie, in the sense of idiopathic) amyloidosis, in which no associated clinical condition was identified, and secondary amyloidosis, which is associated with chronic inflammatory conditions. (medscape.com)
Descriptive terms such as primary amyloidosis, secondary amyloidosis, and others (eg, senile amyloidosis), which are not based on etiology, provide little useful information and are no longer recommended. (medscape.com)
While this control addresses whether the secondary antibody reagents are a source of staining, inadvertent binding of the primary antibody to the tissue can occur. (vectorlabs.com)
2) Use of an isotype control (e.g. "non-immune" mouse IgG), matched to that of the primary antibody and applied at the same protein concentration as the primary antibody, is probably the most widely used negative control. (vectorlabs.com)
Finally, some conditions secondary to acquired immunodeficiency are also described because they need to be recognized in addition to the primary diseases. (medscape.com)

In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)

Native or wild-type quaternary protein structure is usually born from a single translated protein sequence with one ordered conformation with downstream protein interactions. (medscape.com)

Using DAS-ELISA, the detection limit of recombinant PDCoV N protein and virus titer were approximately 0.5 ng/mL and 10 3.0 TCID 50 /mL, respectively. (chxa.com)
The coating antibody at 1 µg/ml concentration and 1:500 dilution of enzyme conjugate were found to be effective and economical in the detection of recombinant N protein of TSWV and the virus present naturally in the infected hosts. (chxa.com)

This antiserum specifically reacted with recombinant N protein as well as TSWV infected hosts, but not with groundnut bud necrosis orthotospovirus (GBNV) as well as capsicum chlorosis orthotospovirus (CaCV) infecting tomato and chilli plants. (chxa.com)
A protein solution with the properties of a specific antiserum to the triphenylmethane dye methyl blue has been made by treating a solution of bovine gamma-globulin and the dye with alkali and then slowly neutralizing the alkali. (caltech.edu)
F(ab')2 fragment PE conjugated secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. (rockland.com)

The predicted MCEPH protein is truncated at amino acid 230 out of 495. (biomedcentral.com)
Newly produced antibody, like the other plasma proteins, became readily labelled with S35 amino acids within a few hours. (caltech.edu)

Anamnestic antihapten responses were obtained to trinitrophenyl (TNP) when rabbits sensitized to trinitrophenyl-hemocyanin (TNP-KLH) were challenged with TNP-heterologous protein conjugates. (caltech.edu)
PCA clustering showed that the overall immune responses were altered by immunization, but no substantial changes were observed following direct worm challenge with either Ascaris suum or Trichuris suis . (frontiersin.org)

After immunization, serum anti-CENP-A antibody was detected by indirect immunofluorescence assay (IIFT) and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)

Other specific initiatives were conducted such as evaluation of the sublingual route for immunization, investigation of the Principal Immunodominant Domain (PID) of gp41, and direct HIV inhibition with an Ag family which demonstrated in vitro antiviral activities comparable to the antiretroviral drug T20 (Fuzeon). (europa.eu)

A secondary immune response was seen in 18.4% of patients. (who.int)

Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in virus replication, virulence, and host range. (asm.org)

The secondary objectives will be to assess all-cause mortality at Day 60, survival at day 31, number of subjects experiencing serious adverse events (SAEs) by day 31, SAE-free survival, time to recovery (evaluated until day 60), and time to oxygen requirement equal or below 40% oxygen. (therapeuticsolutionsint.com)

Both treatments favourably and similarly modified plasma MDA (lipid peroxidation marker), GC (protein oxidation marker) and CAT activity. (iosrphr.org)

Anatomy1

Organisms2

Diseases2

Chemicals and Drugs28

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes6

Health Care4

Antibodies17

Immunity6

Active immunization2

Antigens6

Vaccine11

Mice4

Humans1

Antigen9

Recombinant proteins1

Peptides2

Homologous2

Vaccines1

Serum proteins1

Vaccination2

Immunogenic1

Adjuvant3

Polyclonal2

Programme1

Cellular1

Molecule3

Efficacy1

Specificity1

Receptor3

Lymphoid3

20221

Immunoglobulin3

Membrane1

Encodes2

Amyloid4

Poliovirus1

Viral2

Centromere1

Recommendations1

Genes1

Human3

Cells5

Tissues1

Primary5

Fluorescent1

Conformation1

Detection2

Antiserum3

Amino2

Responses2

Assay1

Specific1

Immune response1

Roles1

Objectives1

Marker1