Protein expression expression patterns expressed sequence tags. Medical search. Frequent questions

The increasing numbers of available genomic sequences of ectomycorrhizal (ECM) fungi enable deeper insights into the characteristics of MT genes in these fungi that form the most important symbiosis with the host trees in forest ecosystems. (bvsalud.org)
An allele is one of two or more versions of DNA sequence (a single base or a segment of bases) at a given genomic location. (genome.gov)
These tag-based technologies, which do not require a priori knowledge of genomic sequence, are well-suited for transcriptional profiling. (biomedcentral.com)

Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). (nih.gov)
In silico comparison of cDNA libraries identified aphid genes with tissue-specific expression patterns, and gene expression that is induced by feeding on Nicotiana benthamiana. (nih.gov)

Hence, it means that human testis could also play a crucial role in human speciation if these two tissues exhibit similar gene expression patterns. (karger.com)
Determining the large scale anatomy of gene expression patterns of various human tissues could draw a more convincing conclusion, and better our understanding of the correlation/inter-correlation among different tissues. (karger.com)
Among the 17 tissues, the highest similarity in gene expression patterns was between human brain and testis, based on DDD and clustering analysis. (karger.com)
Bortoluzzi S, Alessi FD, Romualdi C, Danieli GA: Differential expression of genes coding for ribosomal proteins in different human tissues. (karger.com)
Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit development. (biomedcentral.com)
Genes specifically expressed in fruit tissues were selected and their isolated promoter regions were fused with the GUSPlus reporter gene for evaluation in transgenic plants. (biomedcentral.com)
Among the five candidate citrus promoters characterized in this study, the CitSEP promoter showed a fruit-specific expression pattern, while the CitWAX and CitJuSac promoters exhibited high fruit-preferential expression with strong activity in the fruit, weak activity in floral tissues and low or undetectable activity in other tissues. (biomedcentral.com)
The CitVO1, CitUNK and PamMybA promoters, while exhibiting strong fruit-preferential expression, also showed consistent weak but detectable activity in leaves and other vegetative tissues. (biomedcentral.com)
As described in Materials and Methods, we constructed a cDNA library from fat body tissues of E. coli - challenged A. mylitta larvae and randomly sequenced a large number of inserts from the library. (biomedcentral.com)
Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. (origene.com)
Because brain tissue storage and degradation after death can affect DNA methylation and postmortem brain samples are limited , other researchers have examined methylation patterns in more readily available tissues, such as the placenta and cord blood. (spectrumnews.org)
Different tissues have different functions and thus differences in which genes are expressed. (spectrumnews.org)
To address that issue, translatome analysis [ 18 ] has been applied to identify sex-specific genes expressed during pollination, but this strategy needed large amounts of tissues from a transgenic line expressing tagged ribosomes in pollen and fine techniques of biochemistry. (biomedcentral.com)
Among the matched sequences, ESTs were identified from tissues other than berries or from berries at different developmental stages. (biomedcentral.com)
One orthogonal approach is to compare antibody staining intensities to RNA-Seq data from the same samples, over multiple tissues or cells with varying expression of the target protein. (atlasantibodies.com)
For each antibody, two tissues or endogenous cell lines are chosen for the validation, one with high RNA expression and the other with low or no RNA expression of the target. (atlasantibodies.com)
If the two antibodies generate a similar staining pattern when compared in a set of relevant tissues, the antibodies validate each other. (atlasantibodies.com)
Antibody stainings across relevant positive and negative tissues are similar between the two, and the antibodies validate each other's staining pattern in IHC. (atlasantibodies.com)

We identified 719 unique ESTs from a total of 1412 sequences so generated. (biomedcentral.com)
The results reveal significant differential expression of a total of 236 ESTs, 96 transcripts differentially abundant after 1 day of physical contact with the fungus, 134 transcripts after 5 days and only 6 after 15 days at early stages of mantle formation on emerging lateral roots. (biomedcentral.com)
Additional MPSS signatures not matching to known grape ESTs can extend our knowledge of the V. vinifera transcriptome, particularly when these data are used to assist in annotation of whole genome sequences from Vitis vinifera . (biomedcentral.com)

Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
Thus, this study demonstrates that our transgenic model can uncover important in vivo APP-interacting proteins that will contribute to our understanding of APP processing in in vivo settings. (jneurosci.org)
To explore the means by which prions are transmitted with high efficiency among cervids, we examined prion infectivity in the apical skin layer covering the growing antler (antler velvet) by using CWD-susceptible transgenic mice and protein misfolding cyclic amplification. (cdc.gov)
The fact that CWD prion incubation times in transgenic mice expressing elk prion protein are consistently more rapid raises the possibility that residue 226, the sole primary structural difference between deer and elk prion protein, may be a major determinant of CWD pathogenesis. (cdc.gov)

Gene expression patterns in human liver cells exposed to tetrachloroethylene and it's metabolite using microarray analysis. (cdc.gov)
In our laboratory, studies have been conducted to determine changes in gene expression patterns in cultured human cells exposed to TCE and TCA. (cdc.gov)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
This gene product stimulates the motility of tumor cells, has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. (betalifesci.com)
HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
These protein subunits can assemble into virus particles without viral RNA genome in mammalian cells to study virus-cell entry 2 and to develop vaccines 3 . (nature.com)
VLPs are highly immunogenic and can induce elevated titers of neutralizing antibodies, even without adjuvants, thanks to the maintenance of the native conformation of viral proteins presenting repetitive epitopes to the host immune system cells. (nature.com)
The synthetic protein is designed to be secreted outside E. coli cells, and therefore can be purified using a His-tag from the cell supernatant (thereby reducing the need to lyse the cells for purification). (llnl.gov)
Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)
An expressed sequence tag (EST) library was constructed from mRNA obtained from fat bodies of A. mylitta larvae that had been challenged by infection with Escherichia coli cells. (biomedcentral.com)
Fatty acid (FA) profile assays revealed that HpDGAT2A, HpDGAT2D, and HpDGAT2E, but not HpDGAT2B, preferred monounsaturated fatty acyl-CoAs (MUFAs) for TAG synthesis in yeast cells, and showed a preference for polyunsaturated fatty acyl-CoAs (PUFAs) based on their feeding strategy. (researchsquare.com)
Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separations - Regis Dieckmann, Yohanne Coute, Denis Hochstrasser, Jean-Jacques Diaz and Jean-Charles Sanchez 11. (nhbs.com)
It is also possible to capture the entire population of proteins in cells that bind to metals such as copper or iron, the so-called metalloproteome," adds Dr. Boronowsky. (genengnews.com)
Such global gene expression patterns can be represented by a "transcriptome", which reveals the identity and the level of expression of each expressed gene in a defined population of cells (Velculescu et al. (scite.ai)
Lane 1: marker, lane 2: negative control (vector only transfected HEK293T lysate), lane 3: ACY3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408962). (atlasantibodies.com)
The pattern and timing of gene expression can be determined by creating recombinant DNA with a reporter gene under the control of a cis -regulatory sequence of interest and introducing it into cells or an organism. (jove.com)
Since the gene of interest and the reporter gene have the same cis -regulatory sequence, they are expressed in the same cells and at the same time. (jove.com)
The cells expressing GFP glow green when excited by wavelengths of blue to the ultraviolet range, while those expressing RFP glow red when excited by wavelengths of 488 nm or 532 nm. (jove.com)
The cells expressing the Luc gene produce luciferase enzymes that catalyze a reaction with luciferin to produce light. (jove.com)
Altered gene expression in these nontransformed stromal cells provides potential targets for therapy. (aacrjournals.org)
Analysis of heterologous expression systems of rOAT1 in oocytes and culture cells revealed that rOAT1 has the ability to transport anionic drugs, such as β-lactam antibiotics, NSAIDs, methotrexate, and antiviral drugs, as well as various endogenous organic anions and exogenous substances. (aspetjournals.org)
In the first phase, sender cells grow and accumulate synthase protein. (github.io)
This propagation ends at the third phase, when cell growth and protein production slow down such that the amplifier cells cannot produce enough synthase protein to sustain signal transmission. (github.io)
Cells in a consortium will transition between these phases at different times, compounding the spatially heterogeneity of the consortium's demographic patterning to the gene expression capacity. (github.io)
Protein quantity is also diminished through dilution as cells grow and divide. (github.io)
Sofia Elmroth and her research team primarily study microRNAs, which are molecules that influence protein levels in the cells of the body indirectly by binding to messenger RNA. (lu.se)
Sequencing of DNA gives a direct picture of our genetic make-up, while sequencing of the RNA that is naturally present in cells gives a picture of which parts of our non-coding DNA are used and in what way. (lu.se)
The large proteoglycan molecules made up of protein cores, and GAG branches are secreted by cells and constitute a significant fraction of the extracellular matrix of connective tissue. (medscape.com)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

Genes contributing to the similarity include ribosomal protein (RP) genes as well as genes involved in transcription, translation and cell division. (karger.com)
This antiserum gave a similar immunofluorescence pattern in T. brucei to those of an epitope-tagged ribosomal protein, QM. (gla.ac.uk)

Mouse IL-17C cDNA encodes a 194 amino acid (aa) residues protein with a putative 14 aa signal peptide (5). (rndsystems.com)
Of the four putative novel defence proteins (DFPs) annotated by PSI-BLAST three showed similarity to extracellular matrix proteins from vertebrates implicated in innate immunity, while the fourth was similar to, yet distinct from, the anti-microbial protein cecropin. (biomedcentral.com)
The present study has identified many potential immune-related genes in A. mylitta some of which are vertebrate homologues and others are hitherto unreported putative defence proteins. (biomedcentral.com)
Previous studies have identified four putative DGAT2-encoding genes in H. lacustris , and only HpDGAT2D allowed the recovery of TAG biosynthesis, but the engineering potential of HpDGAT2s in TAG biosynthesis remains ambiguous. (researchsquare.com)
Each sequence from this database was used to search public protein databases to assign putative function to as many database entries as possible. (usda.gov)
The hOAT3 cDNA consisted of 2179 base pairs that encoded a 543-amino-acid residue protein with 12 putative transmembrane domains. (aspetjournals.org)

Following recognition of TLR ligands, TLRs elicit innate immunity by activating multiple intracellular signaling cascades including variable adaptor proteins and transcriptional factors. (frontiersin.org)
We report an unanticipated transcriptional activity triggered in stigma upon incompatible pollination and show that following compatible interaction, components of the pattern-triggered immunity (PTI) pathway are induced on the female side. (biomedcentral.com)
This permits the assumption that the ratio of co-transcriptional protein amounts is constant, a useful assumption when performing parameter inference (See Appendix A ). (github.io)

First, Tim Graubert and colleagues describe the whole-genome sequencing of an MDS-derived secondary AML tumor and a matched normal (skin) sample. (massgenomics.org)

Biotin labeled cRNA transcripts were synthesized, fragmented and hybridized to HuGeneFL GeneChip probe arrays representing more than 6800 human genes and expressed sequence tags. (cdc.gov)

The nucleotide sequence of a fragment of the T. brucei GPI8 gene has also been obtained. (gla.ac.uk)

The molecular mechanisms underlying important physiological processes of the species were examined through differential gene expression analysis for copepodite stage 5 (CV) and female C. finmarchicus obtained from surface and deep waters in Gulf of Maine, by using an Expressed Sequence Tag (EST) microarray. (uconn.edu)
These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. (nih.gov)
The expression patterns of the cold-responsive transcripts identified by SAGE were confirmed by microarray analysis. (scite.ai)
The recent DNA microarray technique allows large-scale quantitative gene expression analysis. (scite.ai)
This database will facilitate the design of microarray and other experiments to study screwworm gene expression on a larger scale than previously possible. (usda.gov)

Binding response kinetics between Human ACE2 (18-652) Recombinant Protein (hFc-Tag) (immobilized) and SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (in solution, at indicated concentrations). (cellsignal.com)
Values on y-axis indicate binding response signals recorded for 4 different concentrations of SARS-CoV-2 Spike RBD (multimeric) (319-591) Recombinant Protein (8xHis-Tag) #17862 (125, 250, 500 and 1000 nM). (cellsignal.com)
The purity of Human ACE2 (18-652) Recombinant Protein (hFc-Tag) was determined by densitometry after SDS-PAGE of 2 μg of protein, followed by staining with Coomassie Blue. (cellsignal.com)
The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. (betalifesci.com)
Recombinant Human CEACAM-5/CD66e (145-322) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro145-Pro322. (kactusbio.com)
These antibodies detected recombinant protein but failed to detect protein in L. mexicana cell lysates. (gla.ac.uk)
Example of recombinant expression validation in Western blot using the Anti-ACY3 antibody (HPA039219). (atlasantibodies.com)
This recombinant gene is introduced into different cell types, and both the gene of interest and the reporter gene are allowed to express. (jove.com)
This recombinant DNA was introduced into the worm using a microinjection, and the gene was expressed, similarly to β-tubulin. (jove.com)

The ancillary domain, which mediates binding to ECM components and specific protease substrates, consists of a conserved central thrombospondin type 1 sequence repeat motif (TSR), a cysteine-rich domain and a spacer domain. (encyclopedia.pub)
Metallothioneins (MTs) are small, cysteine-rich, heavy metal-binding proteins involved in metal homeostasis and detoxification. (bvsalud.org)

In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. (frontiersin.org)
More than half of L. bicolor mycocypin-encoding genes are differentially expressed during symbiosis or fruiting body formation. (bvsalud.org)

To investigate the APP in vivo interactome in an unbiased manner, we generated mice that harbor a mouse prion protein promoter-driven cDNA encoding human APP-695 fused to a C-terminal affinity tag. (jneurosci.org)
Bioassays in Tg mice expressing deer prion protein (PrP) ( 4 ) and newly created Tg mice expressing elk PrP, demonstrated low levels of CWD prions in antler velvet. (cdc.gov)

Integral membrane protein 2C is a protein that in humans is encoded by the ITM2C gene. (wikipedia.org)
H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. (nature.com)
SARS-CoV-2 is composed of four essential structural proteins: the spike (S) glycoprotein, membrane (M) protein, envelope (E) protein, and nucleocapsid (N) protein, as well as multiple accessory and non-structural proteins 1 . (nature.com)
The PEG membrane provides high biological particles separation and prevents sample for clogging due to the strong hydration of functional polymers layer and their resistance to protein adsorption. (llnl.gov)
AD is pathologically characterized by the deposition of pathogenic Aβ peptides that are derived from larger integral membrane proteins, termed β-amyloid precursor proteins (APPs). (jneurosci.org)
Aβ is liberated from type I integral membrane proteins, termed β-amyloid precursor proteins (APPs), by the concerted action of β-secretase (BACE1) and γ-secretase (for review, see Selkoe, 2002 ). (jneurosci.org)
In higher plants and microalgae, there are four major groups of DGATs: (1) the membrane bound form of DGAT1, (2) the membrane bound form of DGAT2 sharing low sequence similarity with DGAT1, (3) the soluble type of DGAT3, which is localized in the cytosol, and (4) the dual function of WS/DGAT, which possesses both wax ester and TAG biosynthesis activities [12-18]. (researchsquare.com)
Analysis of Membrane Proteins by Two-Dimensional Gels - Michael Fountoulakis, 15. (nhbs.com)
However, according to Dr. Boronowsky, when membrane proteins are being investigated, 10 or even 14 residues might be employed to overcome the masking effects of any detergent that might have been added to improve protein solubilization. (genengnews.com)
Many eukaryotic cell surface proteins are attached to the plasma membrane by glycosylphosphatidylinositol (GPI) anchors. (gla.ac.uk)
The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)

And that pattern of methyl marks across the genome can influence which genes are active or inactive at any given time. (spectrumnews.org)
Studies of postmortem brains suggest that autistic people have different methylation patterns - not only in specific genes but across the entire genome - than non-autistic people. (spectrumnews.org)
We used an expressed sequence tag and 454 pyrosequencing approach to initiate a study of the genome of the New World Screwworm, Cochliomyia hominivorax (Coquerel). (usda.gov)

In this enhanced validation method, antibody specificity is confirmed by downregulating the target protein on a genetic level using siRNA or CRISPR-Cas9. (atlasantibodies.com)
In this enhanced validation method, antibody specificity is demonstrated by comparing two antibodies targeting different regions of the same protein. (atlasantibodies.com)
Exact knowledge of the antigen sequence for each antibody. (atlasantibodies.com)
In this enhanced validation method, the antibody binding is verified using and over-expressed or tagged version of the target protein. (atlasantibodies.com)
When over-expressing the target protein in a cell line, the antibody is validated by comparing the signal from the over-expressed version with the unmodified endogenous target protein. (atlasantibodies.com)
The pattern displayed by the tagged target protein is matched to the antibody signal. (atlasantibodies.com)
A match confirms that the antibody is recognizing its target protein. (atlasantibodies.com)
The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)

Our study represents systematic work on the characterization of HpDGAT2s by integrating expression patterns, AST/TAG accumulation, functional complementation, and heterologous expression in yeast, plants, and algae. (researchsquare.com)
Further characterization showed that susa2-2 only suppresses the autoimmunity mediated by either CHS1-SOC3 or TN2-SOC3 paired NLR proteins, indicating that SUSA2 is specifically involved in NLR protein SOC3-mediated immunity. (ubc.ca)

Triacylglycerol (TAG) is the principal energy storage form in eukaryotic organisms and represents a promising source of biodiesel production [1]. (researchsquare.com)
This makes them suitable for the purification of proteins from eukaryotic samples, thereby extending the use of polyhistidine proteins from bacterial to more complex expression systems. (genengnews.com)

Significantly higher expression was observed in tumor necrosis factor receptor in the TCA treatment group compared to the parent compound - TCE. (cdc.gov)
LPA receptors and ATX are upregulated in numerous cancer cell types and show expression patterns that correlate with tumor cell invasiveness. (betalifesci.com)
CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. (kactusbio.com)

Generally, TAG biosynthesis takes place in the endoplasmic reticulum, and TAG assembly can be divided into acyl-CoA-dependent and acyl-CoA independent pathways [10]. (researchsquare.com)
There is some overlap in fluorescence patterns between tagged QM and the endoplasmic reticulum marker protein BiP. (gla.ac.uk)

IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). (rndsystems.com)
1997). The transcriptome can be modulated by both external and internal factors, and thereby provide not only information useful for the understanding of the basic cellular biology but also a global view of biological responses over environmental stimuli.Gene expression profiles can be obtained and compared by various methods, such as RNA-DNA hybridization measurements, subtractive hybridization, subtraction libraries, and differential display. (scite.ai)
We have initiated study of the genes of the New World Screwworm, Cochliomyia hominivorax, by synthesizing and sequencing DNA libraries of expressed genes from embryos, 2nd instar larvae, adult males and adult females. (usda.gov)

The sequence of the four nucleotide bases encodes DNA's information. (genome.gov)
One of the well-known reporter genes is one that encodes for a protein called green fluorescent protein - or GFP. (jove.com)
SUSA2 encodes an F-box protein Actin-Related Protein 8 (ARP8) and SUSA3 encodes the chaperone protein HSP90.3. (ubc.ca)

Until early 1990s, renal organic anion transport was thought to be carried out by a few carrier proteins that showed wide substrate specificity. (aspetjournals.org)

At least two antibodies with non-overlapping antigen sequences for each protein target. (atlasantibodies.com)

The protein produces a green fluorescence under ultraviolet light, which enables researchers to track its location within a cell. (jove.com)
As GFP fluorescence was shown in the touch receptor neurons, this is likely a site where β-tubulin is expressed. (jove.com)
Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. (jove.com)

Purification of APP-AT protein complexes and identification of the constituents by mass spectrometry revealed several previously reported APP-interacting proteins as well as proteins involved in synaptic maintenance. (jneurosci.org)
Cube Biotech offers PureCube resins for the purification of His-tagged proteins. (genengnews.com)

Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
In polyhistidine tagging, 6 to 10 histidine residues are added recombinantly to either the protein's C- or N-terminus. (genengnews.com)
Sequence comparison of L. mexicana, yeast and human GPI8 proteins identified two potential active site cysteine residues. (gla.ac.uk)

1995). "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). (wikipedia.org)
A codon is a DNA or RNA sequence of three nucleotides (a trinucleotide) that forms a unit of genetic information encoding a particular amino acid. (genome.gov)
Tags are also sometimes added to cytosines followed by other nucleotides, however. (spectrumnews.org)

To assess the native APP interactome in brain, we generated mice expressing human APP-695 fused to a C-terminal affinity tag (APP-AT). (jneurosci.org)
The addition of an affinity tag allowed us to avoid the use of antibodies targeted toward the intracellular C-terminal tail of APP, a region to which a number of reported interacting proteins bind ( King and Turner, 2004 ). (jneurosci.org)
Alternatively, the target protein is tagged by an affinity tag or a fluorescent protein. (atlasantibodies.com)

The authors undertook systematic sequencing of U2AF1 exons in 150 MDS cases, and found that 8.7% had mutations at Ser34. (massgenomics.org)

The Autotaxin protein is important for generating the lipid signaling molecule lysophosphatidic acid (LPA), which is a potent mitogen, which facilitates cell proliferation and migration, neurite retraction, platelet aggregation, smooth muscle contraction, actin stress formation and cytokine and chemokine secretion. (betalifesci.com)

An anticodon is a trinucleotide sequence located at one end of a transfer RNA (tRNA) molecule, which is complementary to a corresponding codon in a messenger RNA (mRNA) sequence. (genome.gov)
Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein. (genome.gov)
Here we performed mRNA profiling using Massively Parallel Signature Sequencing (MPSS) and combined it with available Expressed Sequence Tag (EST) data. (biomedcentral.com)
Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)

In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. (nih.gov)
An amino acid is the fundamental molecule that serves as the building block for proteins. (genome.gov)
The deduced amino acid sequence of hOAT3 showed 36 to 51% identity to those of other members of the OAT family. (aspetjournals.org)
The resulting expression cassette, CerPrP-E226, highlights the single amino acid difference between deer and elk PrP at this position ( 5 ). (cdc.gov)
SLC6A19 is a sodium-dependent and chloride-independent neutral amino acid transporter, expressed predominately in the kidneys and intestine. (medscape.com)
Cloning and expression of the mouse SLC6A19 gene demonstrated that this transporter has all the properties of the amino acid transport system B 0 AT1. (medscape.com)

IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). (rndsystems.com)

Commonly used reporter genes are - GFP (green fluorescent protein gene), lacZ (β- galactosidase gene), RFP (red fluorescent protein gene), and Luc (luciferase gene). (jove.com)

Over one hundred vaccines using varied approaches including protein subunit, viral vector, RNA, DNA, inactivated virus, attenuated virus, and virus like particles (VLPs) platforms are in clinical trials. (nature.com)
LLNL researchers have designed a synthetic, concatemeric bacterial expression vector that expresses a protein sequence that can be digested into a single peptide. (llnl.gov)
The coding sequence was inserted into the MoPrP.Xho expression vector, and the purified transgene was microinjected into pronuclei of fertilized FVB/ Prnp 0/0 oocytes. (cdc.gov)

The MPSS dataset also revealed evidence of antisense expression not previously reported in grapes but comparable to that reported in other plant species. (biomedcentral.com)
Identical LVA-ssrA degradation tags were appended to the coding sequences of the synthase, repressor, and reporter proteins to promote equal degradation rates, represented in the model as \(\rho\) with subscripts indicating the associated model species. (github.io)
The combined production and degradation terms result in the governing equation of the model's protein species. (github.io)

Transcription analysis showed that the expression levels of the HpDGAT2A , HpDGAT2D , and HpDGAT2E genes markedly increased under high light and nitrogen deficient conditions with distinct patterns, which led to significant TAG and EAST accumulation. (researchsquare.com)

Up-regulation of genes involved in cell division, protein synthesis and mating were remarkable in deep females and CVs. (uconn.edu)
Functional complementation demonstrated that HpDGAT2A, HpDGAT2B, HpDGAT2D, and HpDGAT2E had the capacity to restore TAG synthesis in a TAG-deficient yeast strain (H1246) showing a large difference in enzymatic activity. (researchsquare.com)
However, genes involved in energy and protein synthesis, which are prevalent in leaves, were expressed at a relatively low level. (scite.ai)

Previous data has reported similarity between human brain and testis gene expression patterns. (karger.com)
Present results provide evidence to support the proposal that human testis and brain share the highest similarity of gene expression patterns. (karger.com)

Extraction and Solubilization of Proteins for Proteomic Studies - Richard M. Leimgruber 2. (nhbs.com)

The differential gene expression data analysis was performed using GeneChip 4.0 software. (cdc.gov)
This study provides a differential gene expression profile between TMJ fibrous and bony ankylosis. (medscimonit.com)

To determine whether these eight MTs functioned as metal chelators, we expressed them in the Cu- and Cd-sensitive yeast mutants, cup1∆ and yap1∆, respectively. (bvsalud.org)
Two of the proteins involved in this step have been identified in yeast and mammals. (gla.ac.uk)
The predicted protein shares 31% identity with yeast and human homologues. (gla.ac.uk)

Microbial pathogen recognition relies on several classes of germline-encoded pattern recognition receptors (PRRs), including C-type lectin receptors, nucleotide oligomerization domain-like receptors (NLR), RIG-I-like receptors (RLRs), and toll-like receptors (TLRs) ( 6 ). (frontiersin.org)
Most NLRs guard host proteins that are the direct targets of pathogen effectors. (ubc.ca)

Two key mechanisms in this context are methylation and acetylation, both of which involve modifications to the DNA molecule or to the histone proteins around which DNA is wound. (prohealth.com)
Methylation generally serves to suppress gene transcription, meaning that genes with methyl tags are less likely to be expressed. (prohealth.com)
DNA methylation alters glucocorticoid receptor expression through modifications of chromatin structure. (deepdyve.com)
Most methyl tags are deposited onto the DNA nucleotide called cytosine (C) whenever it occurs next to the nucleotide guanine (G). This CpG methylation begins during gestation and can change across the lifespan. (spectrumnews.org)
Do autistic people have different patterns of methylation? (spectrumnews.org)
Other autism-related patterns include higher levels of methylation marks in genes involved in regulating the immune system, neurons and synaptic signaling. (spectrumnews.org)

The biological and pathological functions of ADAMTS proteases are determined broadly by their respective substrates and their interactions with proteins in the pericellular and extracellular matrix. (encyclopedia.pub)

Yigiterhan, Ebru Unal, "Population Genetic Structure and Gene Expressions Patterns of the Copepod Calanus finmarchicus in the North Atlantic Ocean" (2011). (uconn.edu)
Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. (nih.gov)
Use of these fruit specific/preferential promoters for genetic engineering can help with precise expression of beneficial genes and help with accurate prediction of the activity of new genes in host fruit plants. (biomedcentral.com)
These results (1) update the gene models of HpDGAT2s , (2) prove the TAG biosynthesis capacity of HpDGAT2s, (3) show the strong preference for MUFAs and PUFAs, and (4) offer target genes to modulate TAG biosynthesis by using genetic engineering methods. (researchsquare.com)
Further understanding of the pathways and regulatory mechanisms involved in TAG accumulation will facilitate the genetic engineering of microalgae [7-9]. (researchsquare.com)
Autosomal dominant is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
Autosomal recessive is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
Studies from the past decade implicate an additional layer of 'epigenetic' complexity: chemical tags called methyl groups laid on top of a person's genetic code. (spectrumnews.org)
In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)
Co-transcribed proteins are expressed proportionally to one another, the proportionality being dependent on their genetic context and RBS sequence. (github.io)

You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. (betalifesci.com)
Functional classification of the expressed genes reveals that those involved in cellular biogenesis such as polygalacturonase, pectate lyase, and pectin methylesterase make up more than 40% of the total transcripts. (scite.ai)
Our results strongly suggest that poor accumulation of proteins that play a role in stress tolerance may be why Arabidopsis pollen is cold sensitive.The functional and biochemical features of specific cell types are determined by their particular gene expression profiles. (scite.ai)

Thus, reporter genes "report" the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell. (jove.com)

We have characterized the global gene expression patterns of Arabidopsis pollen using Serial Analysis of Gene Expression (SAGE). (scite.ai)
Interestingly, the number of unique tags in pollen was low compared with the SAGE library of the leaf constructed on a similar scale. (scite.ai)
The expression level of the great majority of transcripts was unaffected by cold treatment at 0°C for 72 h, whereas pollen tube growth and seed production were substantially reduced. (scite.ai)
Interestingly, many genes thought to be responsible for cold acclimation such as COR, lipid transfer protein, and ␤-amylase, that are highly induced in Arabidopsis leaves, were only expressed at their normal level or weakly induced in the pollen. (scite.ai)

The heterologous expression of HpDGAT2D in Arabidopsis thaliana and Chlamydomonas reinhardtii significantly increased the TAG content and obviously promoted the MUFAs and PUFAs contents. (researchsquare.com)

D-aminoluciferin is as active as luciferin and provides a free -NH2 group for functionalization to attach peptide sequences corresponding to the cleavage site of a protease. (llnl.gov)
We also show that the associated protease-resistant PrP could be amplified in vitro (for detection by Western blot) by protein misfolding cyclic amplification (PMCA). (cdc.gov)

Moreover, precise spatial (specific expression at the organ, tissue and cell levels) and temporal (developmental stage) expression of the transgene can minimize potential adverse effects or over-taxing the plant by producing unneeded products throughout the plant [ 17 ]. (biomedcentral.com)
Eight MT genes in L. bicolor were cloned, and the expression patterns of their transcripts at various developmental stages based on expressed sequence tag (EST) counts were analyzed. (bvsalud.org)

Epigenetics, the study of changes in gene expression not caused by alterations in the DNA sequence, has revolutionized our understanding of genetics and its impact on health and longevity. (prohealth.com)

Approximately 5,400 clones from each library were sequenced from both the 5' and 3' directions. (usda.gov)

In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
Researchers are finding new RNA all the time because sequencing is now so quick and cheap", says Sofi Elmroth, professor at the Division of Biochemistry and Structural Biology at Lund University. (lu.se)

We also identified several unknown proteins, some of which could have probable immune-related functions based on the results of the ProDom analysis. (biomedcentral.com)
DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
Another technology, Serial Analysis of Gene Expression (SAGE), in part overcomes this limitation. (scite.ai)
SAGE allows simultaneous, comparative, and quantitative analysis of genespecific, 9-to 10-bp sequence tags (Velculescu et al. (scite.ai)
The output of the method is conceptually similar to a possibly more familiar method called Serial Analysis of Gene Expression (SAGE) [ 8 ]. (biomedcentral.com)

These data show that GPI-anchored proteins of L. mexicana are not essential for growth of promastigotes, invasion of macrophages by promastigotes, or infection of mice. (gla.ac.uk)
Tg(CerPrP)1536 +/- mice expressing deer PrP have been described previously ( 4 ). (cdc.gov)
To generate Tg(CerPrP-E226) mice expressing the elk PrP coding sequence, codon 226 of the deer PrP gene ( PRNP ) (GenBank accession no. (cdc.gov)

Alternatively, it can also attract proteins that block gene transcription. (prohealth.com)

Stable transformation in Micro-Tom tomato demonstrated that the candidate promoter regions exhibit differing levels of expression and with varying degrees of fruit specificity. (biomedcentral.com)
Copper shows low specificity and high yield, whereas cobalt is on the other end of the spectrum, generating extremely pure proteins but lower yield. (genengnews.com)
Six to eight histidines are typically used to increase specificity over intrinsic histidine in contaminating proteins. (genengnews.com)
NTA is a tetravalent ligand providing greater specificity but lower yield, while IDA is trivalent-less selective but generally providing more protein. (genengnews.com)

Anatomy32

Organisms31

Diseases3

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes91

Disciplines and Occupations5

Technology, Industry, Agriculture4

Information Science12

Health Care2

Genomic3

CDNA libraries2

Tissues18

ESTs3

Transgenic5

Cells30

Ribosomal protein2

Putative6

Transcriptional3

Whole-genome1

Human Genes1

Nucleotide sequence1

Microarray5

Recombinant9

Cysteine-rich2

Differentially expressed2

Prion protein2

Membrane14

Genome3

Antibody8

Characterization2

Eukaryotic2

Tumor3

Endoplasmic reticulum2

Libraries3

Encodes3

Carrier proteins1

Antigen1

Fluorescence3

Purification2

Residues3

Nucleotides3

Affinity tag3

Systematic1

Secretion1

MRNA5

Amino acid6

Cytokines1

Fluorescent protein1

Vector3

Species3

Distinct1

Synthesis3

Similarity2

Solubilization1

Differential gene expression2

Yeast3

Pathogen2

Methylation6

Extracellular matrix1

Genetic10

Functional3

Gene expression pattern1

Pollen4

Heterologous expression1

Protease2

Developmental2

Changes in gene1

Clones1

Structural3

Analysis5

Mice3

Alternatively1

Specificity4