AnatomyDiseasesChemicals and DrugsPhenomena and ProcessesInformation Science
CatalaseHydrogen PeroxideSuperoxide DismutaseAmitroleAcatalasiaGlutathione PeroxidaseOxidative StressAntioxidantsPeroxidasesReactive Oxygen SpeciesLipid PeroxidationMicrobodiesOxidantsOxidation-ReductionFree RadicalsGlutathioneGlutathione ReductaseFree Radical ScavengersSuperoxidesMalondialdehydeXanthine OxidaseThiobarbituric Acid Reactive SubstancesOxygenPeroxidesGlucose OxidaseLiverParaquatHydroxyl RadicalHemeOrganoidsHydroxidesPeroxidaseOxidoreductasesMolecular Sequence DataPeroxisomesAscorbate PeroxidasesAerobiosis
EnzymesSuperoxideAntibodyAssayBovineAntibodiesConcentrationColiMoleculesLiverHydrogen peroxideCytokinesIncubationSpeciesCorneaReactionProteomicsStarchCarbohydratesHumanAmino acidVariantsEukaryoticMammalianReactiveActivitiesImmuneCopperReactionsFunctionalActivityHeat-shock pStructuresDecreaseMRNAImprove proteinDepletionEffectsInfectionMiceExpressionVivo
Enzymes17
  • One major group of proteins called enzymes are mandatory for essential life. (bartleby.com)
  • One way proteins help our body helps our bodies is that enzymes act as catalyst for chemical reactions in our bodies. (bartleby.com)
  • All enzymes are under the class of protein biomolecule. (ipl.org)
  • Can someone guide me on the references of spectrophotometer methods used to measure enzymes or proteins newly discovered in the blood serum? (researchgate.net)
  • What are the main references (book or research) for one or more, that specify methods for measuring newly discovered enzymes or proteins, in which simple manual methods are used. (researchgate.net)
  • S. Slathia, A. Sharma and S. Choudhary, "Influence of Exogenously Applied Epibrassinolide and Putrescine on Protein Content, Antioxidant Enzymes and Lipid Peroxidation in Lycopersicon esculentum under Salinity Stress," American Journal of Plant Sciences , Vol. 3 No. 6, 2012, pp. 714-720. (scirp.org)
  • The liver sustains a neutral pH of about 7, which creates the best environment for catalase and other enzymes. (meltingpointathens.com)
  • Activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were measured in ellagic acid-treated V79-4 cells. (nutrimedical.com)
  • Most enzymes are protein s, although a few are catalytic RNA molecules . (ne.jp)
  • Some household products use enzymes to speed up chemical reactions: enzymes in biological washing powder s break down protein, starch or fat stains on clothes, and enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew. (ne.jp)
  • Animals and plants contain enzymes which help break down fats, carbohydrates and proteins into smaller molecules the cells can use to get energy and carry out the processes that allow the plant or animal to survive. (educationindex.com)
  • Catalase (Enzymes) Abstract In this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. (educationindex.com)
  • Most enzymes are protein, which is a macromolecule with a unique three dimensions configuration that acts as a catalyst. (ukessays.com)
  • Since enzymes are proteins, they are very sensitive to their environmental factors, such as temperature, level of pH, and concentration of enzyme and substrate in an enzyme-substrate mixture, that may affect the enzymes or its reaction rate. (ukessays.com)
  • Enzymes as proteins that act as catalysts. (powershow.com)
  • Epididymal (visceral) and subcutaneous adipose tissue, gastrocnemius muscle, and plasma samples were collected to measure oxidative stress markers (advanced oxidation protein products (AOPP), oxidized low-density lipoprotein (oxLDL)), antioxidant system markers (ferric-reducing ability of plasma (FRAP), superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities), and prooxidant enzymes (NADPH oxidase and xanthine oxidase (XO) activities, myeloperoxidase content). (hindawi.com)
  • Allergen avoidance strategies including personal protective equipment, engineering controls, protein encapsulation, and reduction of airborne enzyme concentrations are required to mitigate occupational exposure to fungal enzymes. (cdc.gov)
Superoxide6
Antibody1
Assay3
  • catalase functional activity in lysates was determined using a H 2 O 2 activity assay. (bmj.com)
  • I will be thankful if anybody answer my question and send me a clear protocol to measure ABTS Radical scavenging activity of protein samples.I want to measure antioxidant capacity of my samples by ABTS assay. (researchgate.net)
  • Cd and Hg produced an induction of Hsp70 protein as assayed by Western blotting and enzyme-linked immunosorbent assay (ELISA). (cdc.gov)
Bovine2
  • The amino acid sequence of bovine catalase was determined in 1969, and the three-dimensional structure in 1981. (wikipedia.org)
  • So he decided to covalently link PEG to the enzyme bovine liver catalase. (acs.org)
Antibodies3
  • Antibodies are also proteins. (bartleby.com)
  • Abcam: antibodies, proteins, kits. (abcam.com)
  • Cross-reactivity between a streptococcal 60- to 65-kd heat shock protein (hsp) and the oral mucosa has been demonstrated, and significantly elevated levels of serum antibodies to hsp are found in patients with RAS. (medscape.com)
Concentration3
Coli5
  • Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. (iucr.org)
  • An impediment to the successful production of large quantities of natively folded proteins in Escherichia coli is the tendency of many proteins to become insoluble when overexpressed. (iucr.org)
  • Protein aggregation remains a significant problem in E. coli expression systems. (iucr.org)
  • Recombinant human ALAD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. (novusbio.com)
  • Recombinant human CAT / Catalase purified from E. coli. (covalab.com)
Molecules6
  • one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second. (wikipedia.org)
  • To this end, catalase is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less-reactive gaseous oxygen and water molecules. (wikipedia.org)
  • Proteins are also used as transport molecules, such protein is haemoglobin which transports oxygen in red blood cells all around the body. (bartleby.com)
  • Without the help of the many different protein molecules, the human body would not be able to function properly. (bartleby.com)
  • These proteins are constantly at work assembling molecules, metabolizing energy, and fighting off infections. (bartleby.com)
  • Additionally, the company's neural exosome platform can be combined with therapeutics, such as small molecules, siRNAs and proteins, to cross the blood brain barrier and to the site of disease. (wlns.com)
Liver4
  • In 1937 catalase from beef liver was crystallized by James B. Sumner and Alexander Dounce and the molecular weight was measured in 1938. (wikipedia.org)
  • However, "Copper deficiency can lead to a reduction in catalase activity in tissues, such as heart and liver. (wikipedia.org)
  • However, catalase deficiency in mice may increase the likelihood of developing obesity, fatty liver, and type 2 diabetes. (wikipedia.org)
  • Similarly, significant increase in Lipid Peroxidation (LPO) level while induced significant decreases in the activities of liver Catalase (CAT) and Glutathione-S-Transferase (GST). (scialert.net)
Hydrogen peroxide5
Cytokines2
  • [ 1 ] Neutrophils move to the site of invasion by means of chemotaxis, which occurs in response to microbial products, activated complement proteins, and cytokines. (medscape.com)
  • Mitogen-activated protein kinases (MAPKs) comprise a large family of proteins activated by a wide range of proinflammatory cytokines and environmental stress. (molvis.org)
Incubation2
  • Following incubation with AdCL, catalase expression reached maximum at 5-7 days. (bmj.com)
  • The stoichiometry of binding of radiolabeled PCP to the proteins in the incubation mixture in the absence of GSH was 4:1. (aspetjournals.org)
Species1
  • The pH optimum for other catalases varies between 4 and 11 depending on the species. (wikipedia.org)
Cornea1
  • UVB irradiation caused significant damage to the corneas, including apparent corneal ulcer and severe epithelial exfoliation, leading to decrease in the activities of SOD, catalase, GSH-Px, GSH-Rd, and GSH content in cornea, whereas there was increased corneal MDA content as compared with the control group. (molvis.org)
Reaction4
  • The optimum pH for human catalase is approximately 7, and has a fairly broad maximum: the rate of reaction does not change appreciably between pH 6.8 and 7.5. (wikipedia.org)
  • While the complete mechanism of catalase is not currently known, the reaction is believed to occur in two stages: H2O2 + Fe(III)-E → H2O + O=Fe(IV)-E(.+) H2O2 + O=Fe(IV)-E(.+) → H2O + Fe(III)-E + O2 Here Fe()-E represents the iron center of the heme group attached to the enzyme. (wikipedia.org)
  • When radiolabeled PCP was used to inactivate the enzyme and the reaction mixture analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, radioactivity was found to be associated with P450, reductase, and catalase that had been added to protect against oxidative damage. (aspetjournals.org)
  • When GSH was included in the reaction mixtures, analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated a marked decrease in the binding to all three proteins. (aspetjournals.org)
Proteomics1
  • N-myristoylated proteins from VACV and the host were metabolically labelled with myristic acid alkyne during infection using quantitative chemical proteomics. (cdc.gov)
Starch1
Carbohydrates1
  • Fatty acids Carbohydrates, proteins, and fats are the main types of macronutrients in food (nutrients that are required daily in large quantities). (msdmanuals.com)
Human14
  • Human catalase forms a tetramer composed of four subunits, each of which can be conceptually divided into four domains. (wikipedia.org)
  • Protein accounts for about three-fourths of the dry matter in human tissues other than fat and bone. (bartleby.com)
  • The human body uses proteins for growth and to build and repair bones, muscles, tissue, skin, internal organs and blood. (bartleby.com)
  • Proteins are important for the human body. (bartleby.com)
  • Complete sequence and polymorphism study of the human TYRP1 gene encoding tyrosinase-related protein 1. (medlineplus.gov)
  • A recombinant adenovirus vector (AdCL) was used to transfer human catalase cDNA into EC of whole thickness rabbit corneas ex vivo. (bmj.com)
  • For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured. (nutrimedical.com)
  • Most non-human proteins cause adverse immune reactions when injected into people, limiting their therapeutic value. (acs.org)
  • Davis thought a polymer might give non-human proteins a protective cloak. (acs.org)
  • Reacts with human CAT / Catalase. (covalab.com)
  • This study is to investigate the role of p38 mitogen-activated protein kinase (p38MAPK) in tert -butyl hydroperoxide ( t BHP)-induced apoptosis of human trabecular meshwork (iHTM) cells. (molvis.org)
  • Recombinant Human SOD is a 16.8 kDa protein containing 160 amino acid residues including an N-terminal His‐tag. (peprotech.com)
  • Comparative cytotoxicity of cadmium and mercury in a human bronchial epithelial cell line (BEAS-2B) and its role in oxidative stress and induction of heat shock protein 70. (cdc.gov)
  • We have recently shown that the replication of rhinovirus, poliovirus and foot-and-mouth disease virus requires the co-translational N-myristoylation of viral proteins by human host cell N-myristoyltransferases (NMTs), and is inhibited by treatment with IMP-1088, an ultrapotent small molecule NMT inhibitor. (cdc.gov)
Amino acid3
  • One mutation replaces a protein building block (amino acid) in tyrosine-related protein 1 with a signal that prematurely stops protein production. (medlineplus.gov)
  • This mutation, written as Ser166Ter or S166X, affects the amino acid serine at protein position 166. (medlineplus.gov)
  • Nature's design of proteins (Why was that metal or amino acid selected? (lu.se)
Variants1
  • Alternative splicing may result in different protein variants. (wikipedia.org)
Eukaryotic3
  • These data indicate that this eukaryotic cell-free expression system has a high success rate and that the addition of specific reagents can increase the yield of soluble protein. (iucr.org)
  • Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis , traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. (springer.com)
  • Monné M, Chan KW, Slotboom DJ, Kunji ERS (2005) Functional expression of eukaryotic membrane proteins in Lactococcus lactis. (springer.com)
Mammalian1
  • Free reactive oxygen radicals cause damage of mammalian cells by oxidizing fatty acids, protein and DNA. (bvsalud.org)
Reactive1
  • Because the added nucleophile protects against inactivation and protein labeling by PCP, these data suggest that the reactive intermediate may escape from the active site and attack other sites on the P450, as well as other proteins in the milieu. (aspetjournals.org)
Activities4
  • An altered antioxidant defence system marked by elevated SOD, CAT, and GR activities, with decreased GPx and GST activities were observed along with increased protein carbonylation, disturbed redox status in hypothyroid immature rat testis. (hindawi.com)
  • Super-oxide dis-mutase and catalase activities increased because of TiO 2 NPs treatments. (nature.com)
  • 0.05) ameliorate corneal damage and increase the activities of SOD, catalase, GSH-Px, GSH-Rd, and GSH content, and decrease the MDA content in corneas when compared with the UVB-treated group. (molvis.org)
  • Compared with the control, MICT increased GPx and catalase activities and the FRAP level in epididymal adipose tissue. (hindawi.com)
Immune1
  • Frank F. Davis, who first came up with the idea of pegylation-attaching polyethylene glycol (PEG) to a protein to help it escape the body's immune response-died May 19. (acs.org)
Copper1
  • Any heavy metal ion (such as copper cations in copper(II) sulfate) can act as a noncompetitive inhibitor of catalase. (wikipedia.org)
Reactions2
  • Without these proteins, these reactions would take place too slowly to keep us alive. (bartleby.com)
  • these are important proteins that help reactions occur in the body, such as releasing from the food we eat. (bartleby.com)
Functional3
  • The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis . (springer.com)
  • Kunji ERS, Slotboom DJ, Poolman B (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. (springer.com)
  • Genes Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or the code for functional ribonucleic. (msdmanuals.com)
Activity1
  • while decline in catalase (CAT) activity was recorded when compared with the untreated control. (scirp.org)
Heat-shock p1
  • Further, heat-shock protein 70 (Hsp70) expression was observed as a sensitive indicator of cellular stress. (cdc.gov)
Structures3
  • Three-dimensional protein structures of the peroxidated catalase intermediates are available at the Protein Data Bank. (wikipedia.org)
  • Tyrosinase-related protein 1 may also help determine the shape of melanosomes, which are the structures in melanocytes where melanin is produced. (medlineplus.gov)
  • Self-assembly coatings are used to functionalize the surface structures of protein. (bvsalud.org)
Decrease2
  • Correspondingly, analysis of the components of the inactivated sample by reversed-phase HPLC demonstrated that radioactivity was associated with P450, reductase, and catalase, and that there was a marked decrease in the labeling of all three proteins in the presence of GSH. (aspetjournals.org)
  • In the presence of GSH, there was a decrease in immunoreactivity, although binding of PCP to all three proteins was still detected. (aspetjournals.org)
MRNA1
  • Hsp70 protein and mRNA were sensitive to exposure to these metals. (cdc.gov)
Improve protein1
  • The EBR and Put co-applications were able to improve protein content in NaCl stressed plants over only NaCl stressed plants. (scirp.org)
Depletion1
  • Depletion of sulfhydryl groups of cellular proteins and generation of ROS may be involved in metal-induced lung cell damage. (cdc.gov)
Effects4
  • Some humans have very low levels of catalase (acatalasia), yet show few ill effects. (wikipedia.org)
  • To examine the morphological effects of catalase gene transfer in modulation of H 2 O 2 induced injury, transduced corneas were maintained in ex vivo culture and challenged with H 2 O 2 . (bmj.com)
  • These variations of environmental factors are use to conduct experiments to examine the effects it have on catalase and lipase. (ukessays.com)
  • A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
Infection1
  • These proteins help the body defend against infection. (bartleby.com)
Mice2
Expression3
  • However, lack of expression and insolubility remain significant challenges to the expression and crystallization of these proteins. (iucr.org)
  • There is extensive literature on the variables leading to insoluble recombinant expression of proteins. (iucr.org)
  • Due to their unique properties, expression and study of membrane proteins in heterologous systems remains difficult. (springer.com)
Vivo1
  • Ex vivo catalase gene transfer can protect EC from death mediated by H 2 O 2 . (bmj.com)