• Beta-Lactamase Inhibitor Proteins (BLIPs) are a family of proteins produced by bacterial species including Streptomyces. (wikipedia.org)
  • BLIP acts as a potent inhibitor of beta-lactamases such as TEM-1, which is the most widespread resistance enzyme to penicillin antibiotics. (wikipedia.org)
  • This is a drug combination of a beta-lactamase inhibitor with ampicillin. (medscape.com)
  • Clavulanic acid and a novel beta-lactamase inhibitor, BRL36148, both interact specifically with the OXA-2 beta-lactamase by branched pathway mechanisms. (bl.uk)
  • The beta-lactamase could hydrolyze both penicillin antibiotics including ampicillin, benzylpenicillin, and carbenicillin as well as cephalosporin antibiotics including nitrocefin, cephalothin, cephaloridine, and cefoperazone. (lu.se)
  • Penicillins inhibit bacterial cell wall synthesis by binding to penicillin-binding proteins. (medscape.com)
  • Bactericidal activity results from inhibition of cell wall synthesis via an affinity for penicillin-binding proteins. (medscape.com)
  • In the current study, Thomas and her team studied a bacterial protein called metallo-beta-lactamase, which renders many clinical strains of bacteria resistant to all penicillin-like antibiotics. (phys.org)
  • Thus, penicillin G was undermined by swift accumulation of staphylococcal penicillinase, ampicillin by TEM-1 enzyme and modern oxymino cephalosporins by "extended-spectrum" beta-lactamases. (uea.ac.uk)
  • Ampicillin is a penicillin beta-lactam antibiotic used in the treatment of bacterial infections caused by susceptible, usually gram-positive, organisms. (druglib.com)
  • The bactericidal activity of Ampicillin results from the inhibition of cell wall synthesis and is mediated through Ampicillin binding to penicillin binding proteins (PBPs). (druglib.com)
  • By binding to specific penicillin-binding proteins (PBPs) located inside the bacterial cell wall, Ampicillin inhibits the third and last stage of bacterial cell wall synthesis. (druglib.com)
  • Penicillin binding proteins (PBPs) possess transpeptidase, transglycosylase as well as carboxypeptidase activities and mediates the crosslinking of N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) of peptidoglycan, thereby promoting the cell wall synthesis. (longdom.org)
  • Binds to penicillin binding proteins and inhibits final transpeptidation step of peptidoglycan synthesis, resulting in cell wall death. (medscape.com)
  • Its bactericidal activity results from inhibiting cell wall synthesis by binding to one or more penicillin-binding proteins. (medscape.com)
  • While many research labs throughout the world are attempting to create new inhibitors that inactivate metallo-beta-lactamases, Thomas and collaborators instead analyze how these new inhibitors work. (phys.org)
  • Hundreds of potential inhibitors have been reported in the literature, and several patents have been filed dealing with metallo-beta-lactamase inhibitors. (phys.org)
  • Some of the reported inhibitors work by removing a required component of the metallo-beta-lactamase. (phys.org)
  • These same inhibitors may remove this same required component of other proteins in humans, causing serious side effects. (phys.org)
  • Tony Fink's work contributed greatly to our understanding of the mechanisms and active site function of beta-lactamases and this knowledge now informs the search for new beta-lactams and beta-lactamase inhibitors. (uea.ac.uk)
  • iii) an enzyme that inactivated all the antibiotics and was not inhibited by β-lactamase inhibitors. (microbiologyresearch.org)
  • The clinical pipeline of the new antibiotics remains dry due to the production of AmpC beta-lactamases by the bacteria to develop resistance against antibiotics. (nih.gov)
  • The addition of clavulanate inhibits beta-lactamase producing bacteria. (medscape.com)
  • A so-called beta lactamase, TEM1 causes resistance against antibiotics like ampicillin in bacteria. (cscs.ch)
  • Jacobs-Wagner's career started off with a bang when, as a graduate student at the University of Liège in the early 1990s, she discovered that some bacteria can induce the enzyme beta-lactamase when exposed to antibiotics such cephalosporin, rendering them resistant to these drugs. (the-scientist.com)
  • How do bacteria know they are under attack, and how are they able to respond by making protein that inactivates the antibiotics? (the-scientist.com)
  • With the bacterial protein inactivated, the antibiotic-amoxicillin-is left to kill the bacteria, thereby treating the infection. (phys.org)
  • The the Cysteine containing enzymatically active version of the domain is also found in the CDC25 class of protein phosphatases and a variety of proteins such as sulfide dehydrogenases and stress proteins such as Senesence specific protein 1 in plants, PspE and GlpE in bacteria and cyanide and arsenate resistance proteins. (embl.de)
  • They are more active against lactamases produced by bacteria. (howmed.net)
  • Ceftriaxone is highly stable in the presence of beta-lactamases, both penicillinase and cephalosporinase, of gram-negative and gram-positive bacteria. (medscape.com)
  • Working with experts in protein chemistry, genetics, beta-lactamase biochemistry, cell wall synthesis, and medical microbiology - in Belgium, France, the United States, and Sweden - Jacobs-Wagner discovered a regulatory protein that can sense the peptidoglycans that accumulate when antibiotics disrupt cell wall synthesis, and then activate transcription of beta-lactamase, the enzyme that disarms the drugs. (the-scientist.com)
  • Beta-lactamases are the greatest single source of resistance to beta-lactam antibiotics. (uea.ac.uk)
  • The purpose of this study was to analyze the binding affinity of β-lactam antibiotics with PBPs and β-lactamases by computational docking studies. (longdom.org)
  • Metallo-β-lactamases catalyze the hydrolysis of a broad range of β-lactam antibiotics and are a concern for the spread of drug resistance. (rcsb.org)
  • Susceptibilities to β-lactam antibiotics and β-lactamase content of two groups of Bacteroides strains were compared. (microbiologyresearch.org)
  • Characterisation of a β-lactamase obtained from a strain of Bacteroides fragilis resistant to β-lactam antibiotics. (microbiologyresearch.org)
  • Treatment is usually with penicillinase-resistant beta-lactams, but because antibiotic resistance is common, vancomycin or other newer antibiotics may be required. (msdmanuals.com)
  • Unlike most coagulase-negative staphylococcal species, S. lugdunensis often remains sensitive to penicillinase-resistant beta-lactam antibiotics (ie, methicillin-sensitive). (msdmanuals.com)
  • A sequence comparison of the relevant metallo-beta-lactamases,based on this protein structure, highlights a few well-conserved aminoacid residues. (embl.de)
  • Because metallo-beta-lactamases contain two metal ions we are able to use a variety of spectroscopic techniques to study them," said Thomas. (phys.org)
  • It inhibits bacterial growth, possibly by blocking dissociation of peptidyl tRNA from ribosomes, causing RNA-dependent protein synthesis to arrest. (medscape.com)
  • This agent inhibits bacterial protein synthesis by binding with the 30S and, possibly, the 50S ribosomal subunit(s). (medscape.com)
  • Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery. (invivogen.com)
  • The sequence of the beta-lactamase gene was also determined. (lu.se)
  • Amino acid sequencing of some beta-lactamases has shown that substitution of only a few amino acids in the bla gene leads to high-level resistance against specific cephalosporins. (lu.se)
  • Analysis of two highly expressed genes from Chlorella virus PBCV-1: Protein characterization and the DNA sequences of the major capsid protein gene and the early/late 33-kDa protein gene. (uml.edu)
  • The DNA sequence of the gene coding for the OXA-2 beta-lactamase has been completed. (bl.uk)
  • A specific DNA probe was designed to come entirely from within the coding sequence of the OXA-2 beta-lactamase gene. (bl.uk)
  • Another probe which comprised the whole of the OXA-2 beta-lactamase gene as well as segments of DNA on either side of the gene, was found to hybridise with a number of resistance plasmids. (bl.uk)
  • For this, we constructed translational fusions comprising the first 20 amino acids of the IncA or IncC effectors and a reporter protein (the mature form of TEM-1 beta-lactamase), expressed under the control of the Y. enterocolitica yopE effector gene promoter (PyopE). (europa.eu)
  • Developing computational methods to relate receptors by the similarity of their ligands, rather than by protein sequence or structure. (ucsf.edu)
  • In the other data set, mutants of a bacterial enzyme that controls resistance to penicillins and cephalosporins, TEM-1 beta-lactamase, were expressed in E. coli under the selective pressure of an antibiotic treatment. (frontiersin.org)
  • Azithromycin acts by binding to the 50S ribosomal subunit of susceptible microorganisms and blocks dissociation of peptidyl tRNA from ribosomes, causing RNA-dependent protein synthesis to arrest. (medscape.com)
  • Protein synthesis is effectively blocked in the presence of high levels of lac repressor and the stability of cytotoxic constructs is enhanced. (qiagen.com)
  • Extracting protein alignment models from the sequence database. (embl.de)
  • Confirmation that the OXA-2 beta-lactamase is a serine enzyme has come from the DNA sequence and the interaction with mechanism based inactivators. (bl.uk)
  • A pCb plasmid encoding a beta-lactamase from Haemophilus ducreyi was transferred to Escherichia coli, purified, and characterized. (lu.se)
  • Assessing the conformational changes of pb5, the receptor-binding protein of phage T5, upon binding to its Escherichia coli receptor FhuA. (ibs.fr)
  • We applied GLASS to a dataset of 60,472 Escherichia coli strains and used this to reexamine the longstanding debate about the role of essentiality versus expression level in the rate of protein evolution. (bvsalud.org)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
  • Hypersensitivity Reactions: Hypersensitivity reactions have been reported in patients receiving beta lactam drugs. (nih.gov)
  • Clavulanic acid inactivates a key protein that the bacterium uses to become resistant to amoxicillin. (phys.org)
  • Type cultures produced low levels of β-lactamase and were susceptible to cefoxitin, latamoxef, imipenem and the combination of benzylpenicillin and clavulanic acid. (microbiologyresearch.org)
  • It confirmed that the enzyme belonged to a class A beta-lactamase which had 99% identity to the ampicillin resistance transposon Tn3 of pBR322. (lu.se)
  • RÉSUMÉ L'émergence et la propagation rapide des souches de Klebsiella pneumoniae résistantes aux antibiotiques et porteuses du gène blaKPC codant la production de carbapénèmases ont compliqué la prise en charge des infections des patients. (who.int)
  • Except for the competence protein these proteins bind two zinc ions per molecule as cofactor. (embl.de)
  • The 3-D structure of a zinc metallo-beta-lactamase from Bacillus cereusreveals a new type of protein fold. (embl.de)
  • The 3-D structure of Bacillus cereus (569/H/9) beta-lactamase (EC3.5.2.6), which catalyses the hydrolysis of nearly all beta-lactams, hasbeen solved at 2.5 A resolution by the multiple isomorphous replacementmethod, with density modification and phase combination, from crystals ofthe native protein and of a specially designed mutant (T97C). (embl.de)
  • By contrast, in the case of TEM-1 beta-lactamase mutations, aggregation is linked to a decreased cell fitness due to inactivation of protein function. (frontiersin.org)
  • Nature's design of proteins (Why was that metal or amino acid selected? (lu.se)
  • As negative control, we used a fusion between the first 20 amino acids of a C. trachomatis ribosomal protein and TEM-1 (RplJ20-TEM-1). (europa.eu)
  • Then, we selected 48 genes that may encode uncharacterised effectors (e. g. no detectable amino acid identity to other proteins or with a particular putative biochemical activity) from the genome of C. trachomatis strain L2/434/Bu. (europa.eu)
  • These proteins include thiolesterases, members of the glyoxalase II family, that catalyse the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid and a competence protein that is essential for natural transformation in Neisseria gonorrhoeae and could be a transporter involved in DNA uptake. (embl.de)
  • Emergence of multidrug resistance resulting from β-lactamases constitutes a worldwide threat. (longdom.org)
  • An alpha beta fold found duplicated in the Rhodanese protein. (embl.de)
  • The majority of proteins function as monodispersed ordered species dissolved in intra- or extra-cellular aqueous fluids. (frontiersin.org)
  • BLIP is able to inhibit a variety of class A beta-lactamases, possibly through flexibility of its two domains. (wikipedia.org)
  • X-ray analysis of the NMC-A beta-lactamase at 1.64-A resolution, a class A carbapenemase with broad substrate specificity. (expasy.org)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing Lactamase_B domain in the selected taxonomic class. (embl.de)
  • no good homologies were observed with any class of beta-lactamase. (bl.uk)
  • A more detailed analysis has revealed from comparison of both primary and predicted secondary structure, that the OXA-2 beta-lactamase may be more closely related to class A enzymes. (bl.uk)
  • For a long time, some proteins and protein families have been presumed to be undruggable. (cscs.ch)
  • This new method could even help with investigating whole protein families, like phosphatases. (cscs.ch)
  • Members of both protein families are involved in the signal pathways that regulate cell proliferation, meaning cell division and thereby generation of new body cells, both for the substitution of damaged or old cells or simply for growth. (cscs.ch)
  • 55699) Ribosomal protein L15e CP001857 CDS Arcpr_0066 complement(55738. (go.jp)
  • Many of these genes encode proteins (e.g., enzymes involved in protein glycosylation) rarely associated with viruses. (uml.edu)
  • The pQE vectors (see table and figure pQE Vectors. Numbered elements listed in table. "> pQE Vectors ) enable placement of the 6xHis tag at either the N- or C-terminus of the recombinant protein. (qiagen.com)
  • Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)
  • Based on the results of this analysis, we also predict that the budding yeast arsenate resistance protein Acr2 and the ORF Ygr203w encode protein phosphatases with catalytic properties similar to that of the Cdc25 family. (embl.de)
  • Exploration of the supramolecular interactions involving tris-dipicolinate lanthanide complexes in protein crystals by a combined biostructural, computational and NMR study. (ibs.fr)
  • Characterisation of the physical properties of the OXA-2 enzyme by analytical ultracentrifugation have not only confirmed the dimeric nature of this beta-lactamase but have also shown that this enzyme forms aggregates at high protein concentrations. (bl.uk)
  • Plasmid stabilization/stability protein-encoding genes were observed in some plasmids but were not established for participating in plasmid segregation. (frontiersin.org)
  • Towards Predicting Protein-Protein Interactions in Novel Organisms. (uml.edu)
  • These are likely to play a role in protein interactions. (embl.de)
  • 2014. Fringe proteins modulate Notch-ligand cis and trans interactions to specifysignaling states. (invivogen.com)
  • we tested if these 22 C. trachomatis proteins with an N-terminal T3S signal were also secreted as full-length proteins after PyopE-dependent expression in Y. enterocolitica. (europa.eu)
  • The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
  • The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
  • Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
  • QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
  • Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
  • several bacterial cold-shock and phage shock proteins. (embl.de)
  • T3S assays performed with T3S-competent Y. enterocolitica or with an isogenic T3S-deficient strain individually expressing the TEM-1 fusions revealed a statistically significant higher level of secretion of 22 fusion proteins relative to RplJ20-TEM-1. (europa.eu)
  • Thirteen proteins showed a statistically significant higher level of secretion than full-length C. trachomatis RplJ. (europa.eu)
  • We also tested secretion of 11 out of these 13 proteins when expressed from an arabinose-inducible promoter in Y. enterocolitica, which revealed T3S-dependent secretion of 7 proteins. (europa.eu)
  • Specifically, they used a so-called Hamiltonian replica exchange-based approach for sampling the possible conformational changes of the target proteins. (cscs.ch)
  • Given the double valence of protein aggregation that can result either in the formation of toxic assemblies or physiologically required organelles, it has long been debated what could determine whether aggregation is beneficial or detrimental from an evolutionary perspective. (frontiersin.org)
  • We know now that many of these proteins feature cryptic binding pockets", says Francesco Gervasio, professor in chemistry and structural biology at the University College London and at the University of Geneva. (cscs.ch)
  • 25637 PKD domain containing protein CP001857 CDS Arcpr_0030 complement(25624. (go.jp)
  • Drugs having beta lactam molecules are not clinically given alone. (howmed.net)
  • Small angle neutron scattering for the study of solubilised membrane proteins. (ibs.fr)
  • Change of dynamics of raft-model membrane induced by amyloid-beta protein binding. (ibs.fr)
  • But finding a regulatory protein that directly interacts with one of the breakdown products - that was unprecedented and really quite exciting. (the-scientist.com)