Protein mutagenesis site directed. Medical search. Frequent questions

Any affinity purification method can be applied, as none of the endogenous proteins in the NEBExpress Cell-free E. coli Protein Synthesis System are tagged. (neb.com)
Various techniques in molecular biology (Biochemical characterization of enzymes, protein purification, SDS-PAGE, Western blotting, construction of appropriate vectors and cloning, site directed mutagenesis etc. (uni-goettingen.de)
The students will have opportunity to practice several of these methods during laboratory work, including site-directed mutagenesis, recombinant protein production and purification, real-time RT-PCR. (lu.se)

The recombinant protein had enzymatic properties similar to those of the plant enzyme. (weedscience.com)
Site-directed mutagenesis was used to selectively eliminate the IgG binding for each epitope and single and multiple mutations were expressed as recombinant GST fusion proteins. (cdc.gov)
The antibodies did not appear to recognize the epitope 63-68 in the recombinant fusion protein. (cdc.gov)
When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)
To achieve our goals we applied an integrated strategy, which involves elements of synthetic organic chemistry, molecular enzymology (recombinant expression and targeted modification of eicosanoid synthesising enzymes by site-directed mutagenesis) and computer-assisted modelling (structural and kinetic modelling and simulation of molecular dynamics). (europa.eu)
We have now shown the dissociation constant for this site to be approximately 160 microM in a recombinant protein fragment consisting of residues 1-85 in human fIX. (lu.se)

Site-directed mutagenesis is one of the most important laboratory techniques for creating DNA libraries by introducing mutations into DNA sequences. (wikipedia.org)
Early attempts at mutagenesis using radiation or chemical mutagens were non-site-specific, generating random mutations. (wikipedia.org)
It has been recently shown that mutations of lens gap junction proteins are associated with certain forms of inherited congenital cataract. (rosalindfranklin.edu)
The current vaccines against COVID-19 either express the full-length S protein (AstraZeneca, CanSino Biologics, Gamaleya), or a stabilized prefusion form of the S protein with proline-stabilizing mutations and/or mutation of the furin cleavage site (Pfizer/BioNTech, Moderna, Johnson & Johnson) ( 1 - 6 ). (frontiersin.org)
All the possible point mutations affecting Trp 552 were investigated by site-directed mutagenesis. (weedscience.com)
Each of two mutations, V46E and Q50E, made to investigate whether the extra negative charge would increase the affinity of the calcium binding site manifested a negligible increase in affinity. (lu.se)
Each of the mutations , ob-tained by multiple site-directed mutagenesis leading to the replacement of amino acid residues lying on the surface of the 3D structure of HlyIICTD, led to a decrease in the interaction of HlyIIC-20 with the mutant form of the protein . (bvsalud.org)
Guerois R, Nielsen JE, Serrano L. Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations. (lu.se)
Capriotti E, Fariselli P, Casadio R. A neural-network-based method for predicting protein stability changes upon single point mutations. (lu.se)
Ho, S. Y. iPTREE-STAB: interpretable decision tree based method for predicting protein stability changes upon mutations. (lu.se)
Casadio, R. A three-state prediction of single point mutations on protein stability changes. (lu.se)
Rooman, M. Fast and accurate predictions of protein stability changes upon mutations using statistical potentials and neural networks: PoPMuSiC-2.0. (lu.se)

Cooperative phosphorylation at these sites by both enzymes was observed in vitro and reflected in vivo by the predominance of the peptide phosphorylated on both sites, as opposed to singly phosphorylated peptides. (nih.gov)
We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)
the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)
Antimicrobial proteins and peptides offer a multifaceted mechanism suitable to fight bacterial resistance. (frontiersin.org)
Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)

We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kDa ribosomal S6 kinase (RSK) and mitogen-activated protein kinase (MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. (nih.gov)
The phosphorylation sites identified for RSK (Ser-362) and MAP kinase (Ser-374) are in the transrepression domain. (nih.gov)
Upon binding of insulin to the insulin receptor (IR), IRS1 is phosphorylated at several YXXM motifs creating docking sites for the binding of PI3Kp85, which activates AKT kinase. (scielo.br)
We aim to study the coupling between LTCC and β adrenergic receptors in different cardiomyocyte microdomains, the distinct involvement of PKA and CAMKII (Ca2+/calmodulin-dependent protein kinase II) and explore how this functional complex is disrupted in heart failure.Methods:Global signaling between LTCCs and β adrenergic receptors was assessed with whole-cell current recordings and western blot analysis. (imperial.ac.uk)
We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes. (jci.org)
Glucose has been reported to increase the de novo synthesis of diacylglycerol (DAG) and translocate and activate protein kinase C (PKC) in rat adipocytes. (jci.org)
It is mediated by the Stt7/STN7 protein kinase, which is activated through the cytochrome b6f complex upon reduction of the plastoquinone pool. (unige.ch)
Using the yeast two-hybrid system, we have mapped one interaction site of the kinase on the Rieske protein of the cytochrome b6f complex. (unige.ch)
This study provides evidence that a switch-protein kinase regulatory network controls availability of σ 66 , the main sigma subunit for transcription in Chlamydia . (plos.org)
In vitro analysis revealed that a putative switch-protein kinase regulator, RsbW, is capable of interacting directly with σ 66 , as well as phosphorylating its own antagonist, RsbV1, rendering it inactive. (plos.org)
The phosphorylation of IκBα by IκB kinase family have been so many identified, but the phosphorylation sites of IκBα by other kinases remain poorly understood. (researchsquare.com)
AURK significantly promotes activation of IκBα at serine 32, but not serine 36 residues, unlike IκB kinase (IKK) family proteins activate both IκBα serine residues. (researchsquare.com)

Targeting key residues of the PT-pathway by site directed mutagenesis significantly alters the pH-activity profile of these variants and in presence of H 2 their cofactor is trapped in an intermediate state indicative of precluded proton-transfer. (nature.com)
A molecular model of the FMDV 3A protein, derived from the nuclear magnetic resonance (NMR) structure of the poliovirus 3A protein, predicted a hydrophobic interface spanning residues 25 to 44 as the main determinant for 3A dimerization. (asm.org)
On the basis of the X-ray crystallographic 3D structure, an approach including site-directed and random mutagenesis is taken to determine functional important residues and regions. (lu.se)

Enzyme activity assays with crude beetle protein extracts revealed that glucosinolate sulfatase (GSS) activity is associated with the gut membrane and has narrow substrate specificity towards the benzenic glucosinolate sinalbin. (nature.com)
Recently we have implemented an in vitro assay that functionally resembles the DNA repair reaction and assays to study protein-protein and protein-DNA interactions. (uu.nl)
Novel cell-based assays were developed to assess antibody-dependence cellular cytotoxicity (ADCC) antibodies against both vaccine and a representative circulation strain HA and NA proteins for the 2014-15 influenza season. (cdc.gov)
The four assays using target cells stably expressing one of the four proteins worked well. (cdc.gov)

Also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, it is used for investigating the structure and biological activity of DNA, RNA, and protein molecules, and for protein engineering. (wikipedia.org)
Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)
Homer proteins are components of postsynaptic density (PSD) and play a crucial role in coupling diverse target molecules. (jneurosci.org)
Molecules can be transported around a cell by so-called motor proteins that move along a network of filaments called microtubules. (elifesciences.org)

Effect of protein fermentation products on gut health assessed in an in vitro model of human colon (TIM-2). (cabi.org)
Semi-synthetic approaches offer an alternative means to manipulate proteins post-translationally, but these modifications have typically been performed in vitro 3 - 8 . (biorxiv.org)
protein interaction and localization studies in vitro and in vivo. (lu.se)
80 (Pt 2), 291-296 (1999) PUBMED 10073687 REFERENCE 3 (bases 1 to 7654) AUTHORS Liu,B., Clarke,I.N. and Lambden,P.R. TITLE Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis JOURNAL J. Virol. (cdc.gov)

The IRS proteins function as adaptors and transmit signals from multiple receptors. (scielo.br)
Applying these to G-Protein Coupled Receptors (GPCRs), which are the single largest family of signaling receptors in human cells. (ucsf.edu)
Developing computational methods to relate receptors by the similarity of their ligands, rather than by protein sequence or structure. (ucsf.edu)
G protein-coupled receptors (GPCRs) belong to the largest class of drug targets. (mdpi.com)

The viral genome encodes four structural capsid proteins (VP1 to VP4) and seven nonstructural (NS) proteins, the leader Lb/ab protease, and proteins encoded in the P2 (2B and 2C) and P3 (3A, 3B, 3C, and 3D) regions ( 9 ). (asm.org)
The Polymerase Incomplete Primer Extension (PIPE) method for cloning and mutagenesis used extensively at the [http://www.jcsg.org Joint Center for Structural Genomics]. (uni-konstanz.de)
Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. (uni-konstanz.de)

Indeed, most live cell applications of PTS utilize single split inteins for the purpose of N/C-terminal tagging 16 - 18 or manipulating protein assembly/expression 19 , 20 . (biorxiv.org)
The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. (researchgate.net)
78 (9), 4827-4837 (2004) PUBMED 15078964 REFERENCE 2 (bases 1 to 7654) AUTHORS Liu,B.L., Viljoen,G.J., Clarke,I.N. and Lambden,P.R. TITLE Identification of further proteolytic cleavage sites in the Southampton calicivirus polyprotein by expression of the viral protease in E. coli JOURNAL J. Gen. Virol. (cdc.gov)

The interaction of POPDC proteins was investigated by co-precipitation, proximity ligation, bioluminescence resonance energy transfer and bimolecular fluorescence complementation. (imperial.ac.uk)
Site-directed mutagenesis was utilised to map the domains involved in protein-protein interaction. (imperial.ac.uk)
in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)
Our major finding suggests that mutual interaction of the two domains appears to be crucial for protein stability and its biological function. (europa.eu)
The LAP [leucine-rich and postsynaptic density-95/Discs large/zona occludens-1 (PDZ)] protein erbin and δ-catenin, a component of the cadherin-catenin cell adhesion complex, are highly expressed in neurons and associate through PDZ-mediated interaction, but have incompletely characterized neuronal functions. (jneurosci.org)
We are interested in the substrate - enzyme interaction, and especially in the interactions that are likely to direct the specificity of the enzyme. (lu.se)

We have previously determined the structure of various domains within this family and characterized how these proteins heterodimerize, how they bind to DNA but the catalytic reaction remains elusive and what determines the preferential heterodimer formation is also not known. (uu.nl)
Contribution of the enzymatic activity was discarded by site-directed mutagenesis at the RNase catalytic site. (frontiersin.org)

Heat labile ribonuclease HI from a psychrotrophic bacterium: Gene cloning, characterization, and site-directed mutagenesis. (researchmap.jp)
02/2010 - 04/2011: Site-directed mutagenesis of alpha-amylase and characterization of biochemical (kinetic and activation) parameters of mutants and wild type. (uni-goettingen.de)

These methods of mutagenesis, however, are limited by the kind of mutation they can achieve, and they are not as specific as later site-directed mutagenesis methods. (wikipedia.org)
This synthetic primer contains the desired mutation and is complementary to the template DNA around the mutation site so it can hybridize with the DNA in the gene of interest. (wikipedia.org)
A large number of methods are available to effect site-directed mutagenesis, although most of them have rarely been used in laboratories since the early 2000s, as newer techniques allow for simpler and easier ways of introducing site-specific mutation into genes. (wikipedia.org)
Inhibition ELISA with the mutant proteins indicated that epitope 126-131 was the dominant epitope, but mutation of epitope 120-125 was also required to eliminate mAb reactivity to Hev b 5. (cdc.gov)
Potapov V, Cohen M, Schreiber G. Assessing computational methods for predicting protein stability upon mutation: good on average but not in the details. (lu.se)
Casadio, R. I-Mutant2.0: predicting stability changes upon mutation from the protein sequence or structure. (lu.se)

For advances in protein structure and enzyme mechanisms by use of affinity labeling and site-directed mutagenesis. (ornl.gov)
We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
Regarding oxygen movement we obtained strong evidence that the main route for oxygen access to the active site of the enzyme follows a transiently interconnected cavities whereby the opening and closure is governed by side chain dynamics. (europa.eu)
Author Manuscript and RNA repair protein AlkB-derived enzyme mixture was first used to remove methylations at the Watson-Crick face. (cdc.gov)

The growth-regulated enzymes responsible for this phosphorylation in early G1 phase of the cell cycle and the sites of phosphorylation have not been identified. (nih.gov)

By Protein Type, the monoclonal antibody segment accounted for the largest revenue share. (globenewswire.com)
An antibody is used to precipitate a protein along with bound proteins. (genomicglossaries.com)
Antibody recognition of the mutant proteins was determined by inhibition ELISA. (cdc.gov)
In pre- and post-vaccine sera from 70 participants in a pre-season vaccine trial, we found ADCC antibodies and a rise in ADCC antibody titer against target cells expressing the 4 proteins but a much higher titer for the vaccine than the circulating HA in both pre-and post-vaccine sera. (cdc.gov)

When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)

The protein engineering market is growing speedily and creating numerous growth opportunities due to the increasing number of engineered proteins and amino acids worldwide. (globenewswire.com)
Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)
In agreement with published findings regarding different HEA and LEA B. napus cultivars, comparison of FAE1 protein sequences from HEA and LEA Brassicaceae revealed one crucial amino acid difference: the serine residue at position 282 of the HEA FAE1 sequences is substituted by phenylalanine in LEA B. napus cv. (unboundmedicine.com)

There have been three types of gap junctional proteins (connexins) identified in the mammalian lens: Cx43, Cx46, Cx50. (rosalindfranklin.edu)

Site-directed mutagenesis is a molecular biology method that is used to make specific and intentional mutating changes to the DNA sequence of a gene and any gene products. (wikipedia.org)
There are numerous methods for achieving site-directed mutagenesis, but with decreasing costs of oligonucleotide synthesis, artificial gene synthesis is now occasionally used as an alternative to site-directed mutagenesis. (wikipedia.org)
The gene thus copied contains the mutated site, and is then introduced into a host cell in a vector and cloned. (wikipedia.org)
Protein engineering refers to the process in which a researcher transforms a protein sequence through insertion, substitution, or deletion of nucleotides in the encoding gene, aiming to obtain a modified protein that is more appropriate for a particular application or purpose than an unmodified protein. (globenewswire.com)

However, since host cells are responsible for glycosylation of influenza virus hemagglutinin and neuraminidase, and glycosylation is important for interactions of these proteins with the immune system, the viruses may have functional differences that are not reflected by their genomic sequence. (cdc.gov)

co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)
Most of the important molecular processes in the cell, such as DNA replication, are performed by large molecular network of protein-protein interactions. (researchsquare.com)
We need to acquire more knowledge of various protein-protein interactions in order to understand biological phenomena, including diseases, and to provide the basis for new therapeutic approaches. (researchsquare.com)
Common among these 10 interactions of organic compounds, their power to detect excess cancer agents is the electrophilic nature of or their metabolites, with DNA and R 1 risk at particular sites. (who.int)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)

the c-terminal membrane-binding portion contributes to the Golgi disassembly and, therefore, functionally similar to the picornavirus 2B protein" /protein_id="NP_786945.1" mat_peptide 1199. (cdc.gov)

Since 2013, the development of the CRISPR/Cas9 technology, based on a prokaryotic viral defense system, has also allowed for the editing of the genome, and mutagenesis may be performed in vivo with relative ease. (wikipedia.org)

the calicivirus NTPase was found in membranous replication complexes" /protein_id="NP_786946.1" mat_peptide 2288. (cdc.gov)

Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in virus replication, virulence, and host range. (asm.org)

New York, May 23, 2023 (GLOBE NEWSWIRE) -- The Protein Engineering Market size is projected to surpass around USD 9,329 Million by 2032, and it is poised to reach a CAGR of 13.6% from 2023 to 2032. (globenewswire.com)
By Technology, the rational protein design segment has dominated the market, and it is growing at the highest CAGR over the forecast period 2023 to 2032. (globenewswire.com)

Within the RNaseA superfamily there is a group of highly cationic proteins secreted by innate immune cells with anti-infective and immune-regulatory properties. (frontiersin.org)
Hemagglutinin with reduced glycan occupancy required a higher concentration of surfactant protein D (an important innate immunity respiratory tract collectin) for inhibition compared to that with normal glycan occupancy. (cdc.gov)

unlike most of the motors in this group (which work together in pairs), Kar3 motor protein subunits each bind to and work with non-motor protein subunits, including one called Cik1. (elifesciences.org)
The head of the non-motor protein cannot bind to ATP, although it can bind to microtubules. (elifesciences.org)

The release of mitochondrial-intermembrane-space pro-apoptotic proteins, such as cytochrome c , is a key step in initiating apoptosis. (biologists.com)

The Popeye domain containing (POPDC) genes encode sarcolemma-localized cAMP effector proteins. (imperial.ac.uk)
The viral protein (VP) 7 and VP4 genes encoding the been described ( 15-21 ). (cdc.gov)

Here, we describe the safety, robust immunogenicity, and potent efficacy elicited in rhesus macaques by a modified vaccinia virus Ankara (MVA) vector expressing a full-length SARS-CoV-2 spike (S) protein (MVA-S). MVA-S vaccination was well tolerated and induced S and receptor-binding domain (RBD)-binding IgG antibodies and neutralizing antibodies against SARS-CoV-2 and several variants of concern. (frontiersin.org)
Selected variants were then produced as purified proteins and characterized by surface plasmon resonance analysis and inhibition of chemotaxis. (unige.ch)
Training dataset of 339 experimentally studied variants in nine proteins and 625 variants from ProTherm. (lu.se)
Training dataset: 339 variants from 9 proteins. (lu.se)
Blind test dataset: 625 variants from 28 proteins. (lu.se)
Training dataset: S1615 - 1615 variants from 42 proteins. (lu.se)
Test dataset - S388 (subset of the first) - 338 variants from 17 proteins. (lu.se)

One of the major aims of Dr. Ebihara's research project are to obtain a more detailed understanding of the biophysical properties of cloned lens gap junctional proteins and to localize the molecular determinants of permeability and gating of the gap junctional channels. (rosalindfranklin.edu)

Chemical or genetic engineering of proteins provides great potential to study protein function and pharmacology or to generate proteins with novel properties. (biorxiv.org)

Hutchison later produced with his collaborator Michael Smith in 1978 a more flexible approach to site-directed mutagenesis by using oligonucleotides in a primer extension method with DNA polymerase. (wikipedia.org)

The Gla module (here with Glu instead of Gla) thus increases the affinity of the EGF module calcium ion binding site. (lu.se)

The Act further directs that where feasible, ATSDR shall develop methods to determine the health effects of substances in combination with other substances with which they are commonly found. (cdc.gov)

To elucidate the ligand-binding surface of the CC chemokine-binding proteins Evasin-1 and Evasin-4, produced by the tick Rhipicephalus sanguineus, we sought to identify the key determinants responsible for their different chemokine selectivities by expressing Evasin mutants using phage display. (unige.ch)
The results also suggest that phage display may offer a useful approach for rapid investigation of the pharmacophores of small inhibitory binding proteins. (unige.ch)

I am also interested in antibodies as special and unique proteins in diagnosis and treatment of different disease. (uni-goettingen.de)

Increasing government initiatives to improve protein engineering capabilities will accelerate market growth. (globenewswire.com)

Following insulin binding, the insulin receptor (IR) autophosphorylates itself and creates docking sites for IRS proteins. (scielo.br)
If the synthesized protein contains a maltose-binding protein (MBP) tag, Amylose Resin (NEB # E8021 ), Amylose Resin High Flow (NEB # E8022 ) or Amylose Magnetic Beads (NEB # E8035 ) can be used. (neb.com)
If the synthesized protein has a chitin binding domain (CBD) tag, Chitin resin (NEB # S6651 ) or Chitin Magnetic Beads (NEB # E8036 ) can be used. (neb.com)
The first EGF-like module of human coagulation factor IX contains a single functionally important calcium ion binding site. (lu.se)
A Large-Scale Test of Free-Energy Simulation Estimates of Protein-Ligand Binding Affinities. (lu.se)

A longstanding effort to do so is by exploiting protein structures to predict new reagents and therapeutic leads (structure-based ligand discovery). (ucsf.edu)
Self-assembly coatings are used to functionalize the surface structures of protein. (bvsalud.org)

These studies elucidate the structure-function relationship of these DNA repair proteins. (uu.nl)

Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)

NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)

Site directed mutagenesis experiments showed that reducing the positive charge of this surface-exposed region of the E protein DII correlated with improved Ae. (cdc.gov)

This assembly results in a spontaneous, essentially traceless splicing reaction that covalently links the two flanking protein segments through native chemical ligation 9 . (biorxiv.org)
In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)

covers a wide spectrum of applied protein sciences with emphasis on upstream R&D engineering to downstream biologics production. (genomicglossaries.com)

found that the Kar3-Cik1 motor binds to and transports a protein complex that is crucial for separating chromosomes during cell division. (elifesciences.org)

In this chapter, electrophilic agents nogenicity from studies of exposed (benzene, 1,3-butadiene, and eth- include direct-acting electrophilic humans. (who.int)

Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
What methods can be used to purify the synthesized protein? (neb.com)

Results: We identified two novel sites of serine phosphorylation at S63 and S262. (researchsquare.com)
Using site directed mutagenesis, the phenylalanine 282 residue was substituted with a serine residue in the FAE1 polypeptide from B. napus cv. (unboundmedicine.com)

Manipulation of proteins by chemical modification is a powerful way to decipher their function or harness that function for therapeutic purposes. (biorxiv.org)

IκBα inhibits the NF-κB by keeping them in a state isolated from the inactive cytoplasmic mask and the nuclear localization signal of NF-κB proteins. (researchsquare.com)

Anatomy10

Organisms14

Diseases1

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes108

Technology, Industry, Agriculture1

Information Science4

Health Care3

Purification3

Recombinant6

Mutations12

Peptides6

Kinase14

Residues3

Assays4

Molecules6

Vitro4

Receptors4

Structural3

Expression3

Interaction6

Catalytic2

Characterization2

Mutation6

Enzyme4

Enzymes1

Antibody4

Molecule2

Amino3

Mammalian1

Gene4

Functional1

Interactions4

Specificity1

Membrane1

Genome1

Complexes1

Nonstructural protein1

20232

Innate2

Bind2

Cytochrome1

Genes2

Variants7

Biophysical1

Pharmacology1

Primer extension method1

Affinity1

Substances1

Phage display2

Antibodies1

Accelerate1

Binding5

Structures2

Structure1

Sequences1

Viral1

Experiments1

Ligation2

Wide spectrum1

Crucial1

Humans1

Methods2

Serine2

Chemical1

Localization1