Promoter sequence repressor proteins. Medical search. Frequent questions

An operon is a cluster of genes that are transcribed together to give a single messenger RNA (mRNA) molecule, which therefore encodes multiple proteins (Fig. 16.11). (presenternet.com)
The regulator gene codes for a repressor protein that binds to the operator, obstructing the promoter (thus, transcription) of the structural genes. (presenternet.com)
The complex binding to the activation sequence activated the target genes. (presenternet.com)
The mammalian Hes basic helix-loop-helix repressor genes play central roles in these processes by maintaining progenitor cells in an undifferentiated state and by regulating binary cell fate decisions. (biologists.com)
In many organs, cell proliferation and differentiation are antagonistically regulated by multiple basic helix-loop-helix (bHLH) activator and repressor genes. (biologists.com)
In particular,the Hes bHLH repressor genes play an essential role in the development of many organs by maintaining progenitor cells and by regulating binary cell fate decisions. (biologists.com)
We show that GtrS and GltR form a two-component system that regulates the expression from the promoters Pedd/gap-1, PoprB and Pglk, which control the expression of genes involved in glucose metabolism and transport. (nih.gov)
The TEAD (1-4) transcription factors comprise the conserved TEA/ATTS DNA binding domain recognising the MCAT element in the promoters of muscle-specific genes. (nih.gov)
RNA-seq identifies a novel set of TEAD4 target genes encoding muscle structural and regulatory proteins and those required for the unfolded protein response. (nih.gov)
Together these results show that TEAD factor activity is essential for normal C2C12 cell differentiation and define a novel and non-redundant role for TEAD4 in regulating expression of the unfolded protein response genes. (nih.gov)
Our in silico findings report that there is cross-regulation between both pathways at the level of gene transcription regulation, mediated by the presence of binding sites for both factors in promoters of genes essential for these pathways. (biomedcentral.com)
We analyzed respective gene promoters' sequences using in silico methods for identification of transcription binding sites, mainly in the NF-κB- and IRF-coding genes. (biomedcentral.com)
Site-specific iterations of biological clues or cumulative issues Compared at other models in the F2 Mechanisms of Inorganic and Organometallic thrive for using the solution of the processes of these cells and network or cycle proteins and Modelling interactions about sufficient capsids and genes of PPT sites. (scoutconnection.com)
Maternal genes involved in dorsoventral (D/V) patterning of the Drosophila embryo interact to establish a stable nuclear concentration gradient of the Dorsal protein which acts as the morphogen along this axis. (silverchair.com)
For example, in E. coli , all of the structural genes that encode enzymes needed to use lactose as an energy source lie next to each other in the lactose (or lac ) operon under the control of a single promoter, the lac promoter. (pressbooks.pub)
Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. (gao-lab.org)
These thermosensors are part of a regulatory network, such as the production of heat shock proteins mediated by sigma factor 32 (RpoH) in Escherichia coli or the transcriptional repressor of heat-shock genes HrcA in Bacillus subtilis (Hecker et al. (springer.com)
In the following paragraphs, we will write genes in italics and their associated proteins in Roman. (wikidoc.org)
Dispersed between the chromosomes are over 20,000 genes, sometimes separated by vast stretches of non-coding DNA, or that which does not encode proteins. (jove.com)
Genes themselves consist of protein-coding exons and non-coding introns. (jove.com)
In eukaryotic genomes, genes are separated by large stretches of DNA that do not code for proteins. (jove.com)
The Arabidopsis genome contains an estimated 340,000 potential MADS binding sites ( de Folter and Angenent, 2006 ), leading to the intriguing question: What is the biological significance of the large number of potential MADS binding sites, and how many target genes do MADS proteins really regulate? (frontiersin.org)
Based on these studies, it appears that MADS proteins may directly regulate thousands of target genes (Figure 1 A), and may serve to integrate different biological processes. (frontiersin.org)
(A) Network representation (Cytoscape) of the reported target genes (represented by blue lines) of the four MADS proteins (yellow circles). (frontiersin.org)
The four MADS proteins share 70 target genes. (frontiersin.org)
Concept of gene, Prokaryotes as genetic system, Prokaryotic and eukaryotic chromosomes, methods of gene isolation and identification, Split genes, overlapping genes and pseudo genes, Organization of prokaryotic and eukaryotic genes and genomes including operan, exon, intron, enhancer promoter sequences and other regulatory elements. (nipaers.com)
However, the great majority of our 3-billion nucleotide long genome does not consist of protein coding genes, which make up only 1-2% of our DNA. (entandaudiologynews.com)
Sequences of previously described binding sites were collected for these five factors present in the cis-regulatory regions of known target genes. (sdbonline.org)

Operator - a segment of DNA to which a repressor binds. (presenternet.com)
Promoter is the site where the RNA polymerase binds to initiate transcription. (drmujeera.com)
The lac operator is a short sequence that binds to the LacI repressor, which prevents transcription. (igem.org)
In the G0/G1 growth phase ZAR2 is predominantly located inside the nucleus of the breast cells, binds to the BRCA2 promoter and inhibits the expression of BRCA2. (biomedcentral.com)
ZAR2 binds to BRCA2/ZAR2 bi-directional promoter in vivo and is responsible, at least in part, for the silencing of BRCA2 gene expression in the G0/G1 phase in human breast cells. (biomedcentral.com)
533 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. (novoprolabs.com)
Cro binds to OR3 preventing access to the RM promoter preventing transcription and production of cI. (wikidoc.org)

AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. (wikipedia.org)
The methyl-CpG-binding protein MeCP2 was discovered over 15 years ago as part of a search for proteins that selectively bind methylated DNA. (portlandpress.com)
or a repressor may not be able to bind to the operator unless it is bound to another molecule, the corepressor. (presenternet.com)
Inducers bind to repressors, causing them to change shape and preventing them from binding to DNA. (presenternet.com)
Inducer produces an i-m-RNA that produces a repressor protein which can bind at the operator region. (drmujeera.com)
In our case, we are characterizing promoters that bind to RNA polymerase from the T7 phage. (igem.org)
It codes for a 'b-HLH' DNA-bind-ing protein which can dimerize and bind to DNA in vitro and to polytene chromosomes in vivo. (silverchair.com)
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). (gao-lab.org)
475) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(493. (novoprolabs.com)
509) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 517. (novoprolabs.com)
571) /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 586. (novoprolabs.com)
Both Int and IHF bind to attP and form an intasome , a DNA-protein-complex designed for site-specific recombination of the phage and host DNA. (wikidoc.org)
Proteins called repressors bind to these regions, preventing polymerase-promoter association, inhibiting transcription. (jove.com)
and bind to CArG-boxes, which are small 8-10 bp DNA sequences composed of 6-8 A or Ts flanked by C and G bases ( de Folter and Angenent, 2006 ). (frontiersin.org)
However, the nucleotides of opposite strands can only bind with one partner: A with T, or G with C. Thus the two strands of DNA are different, but complementary to each other, in sequence. (entandaudiologynews.com)
They bind sequentially to the mRNA, the amino acids are linked by a protein-synthesizing enzyme, and the resulting protein is formed from the correct sequence of amino acids. (entandaudiologynews.com)

Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
Large ribonucleoprotein complexes, termed 'ribosomes', were identified as the sites of protein synthesis. (taylorfrancis.com)
Protein synthesis is effectively blocked in the presence of high levels of lac repressor and the stability of cytotoxic constructs is enhanced. (qiagen.com)
Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein. (genome.gov)
iii) Marshall Nirenberg made cell-free system for protein synthesis and finally the code was deciphered. (infinitylearn.com)
iv)Severo Ochoa enzyme (polynucleotide phosphoryiase) was also helpful in polymerising RNA with defined sequences in a template independent manner (enzyme synthesis of RNA). (infinitylearn.com)
Protein synthesis mechanism with reference of Translation and Transcription d. (slideshare.net)
DPR and NFJS are considered to be allelic disorders, attributable to mutations on the nonhelical (E1/V1) head domain in the KRT14 gene, with consequent premature termination of protein synthesis. (medscape.com)

This lentivirus can be used for regular constitutive high expression (without any induction) or for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present. (nucleusbiotech.com)
Materials and methods: A hybrid promoter was generated by placing the heat shock protein (HSP) 70B′ promoter under the tetracycline-repressor responsive element sequence, and a reporter/therapeutic gene expression plasmid was constructed by placing a reporter/therapeutic gene under the control of this hybrid promoter. (elsevierpure.com)

Evidence from model systems shows that MeCP2 can recruit the Sin3a co-repressor complex to promoters leading to transcriptional repression, therefore suggesting that MeCP2 can interpret the DNA methylation signal to bring about gene silencing. (portlandpress.com)
The KAP1/ KRAB-AFP complex in turn recruits the heterochromatin protein 1 (HP1) family, and other chromatin modulating proteins, leading to transcriptional repression through heterochromatin formation. (embl.de)

In rats, hepatic ketogenic capacity increases rapidly during postnatal development ( 4 ) or fasting ( 5 ) when the mRNA, protein, and activity of HMGCS2 increase in liver ( 6 - 8 ). (aacrjournals.org)
Administration of dibutyryl-cyclic AMP to adult rats increases mRNA, protein levels, and enzymatic activity of HMGCS2 in the liver ( 6, 9 ). (aacrjournals.org)
An anticodon is a trinucleotide sequence located at one end of a transfer RNA (tRNA) molecule, which is complementary to a corresponding codon in a messenger RNA (mRNA) sequence. (genome.gov)
It was Besides referred that the suitable types of pyrimidines of rodents at such and bulk Advances( PPT as the protein of chloride, rice, the receptor of copy after coding the vector) mRNA gene analysis combining the polymorphism authors at older promoters( 8, 10, 11). (scoutconnection.com)
The reverse activity transcribes the ZAR2 mRNA with 966 nt coding sequence which codes for a 321 amino acid protein. (biomedcentral.com)
1.Genetic code is the relationship between the sequence of nucleotides on mRNA and the sequence of amino acids in the polypeptide. (infinitylearn.com)
i)The order and sequence of amino acids are defined by the sequence of bases in the mRNA. (infinitylearn.com)
These sequences typically include the 5′-untranslated region in mRNA including the ribosome binding site (Shine-Dalgarno sequence) and in some cases the start codon (Klinkert and Narberhaus 2009 ). (springer.com)
Introns are excised once the sequence is transcribed to mRNA, leaving only exons to code for proteins. (jove.com)
Thus the DNA code is faithfully transcribed into mRNA and then further translated into protein, mediated by the recognition of nucleotides from DNA to mRNA to tRNA. (entandaudiologynews.com)

This protein functions as both a transcriptional activator and repressor. (wikipedia.org)
Stimulation of TLRs (Toll-Like Receptors) by a pathogen induces activation of signal transduction cascades, which leads to translocation of nuclear factor-κB (NF-κB) to the nucleus [ 1 ], activation of interferon regulatory factors 3/7 (IRF3/7) and/or activator protein-1 (AP-1), which cooperate to induce transcription of various cytokines such as alpha/beta interferon (IFN-α/β) to counteract infection [ 2 - 4 ]. (biomedcentral.com)
Could act as transcriptional activator or repressor. (gao-lab.org)
607 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP. (novoprolabs.com)
In the current study, extensive chromatin immunoprecipitation (ChIP) and ChIP-sequencing analyses revealed that Fpr1 associates specifically with the upstream activating sequences of nearly all RPG (ribosomal protein gene) promoters, presumably in a manner dependent on Rap1 (repressor/activator site binding protein 1). (prolekarniky.cz)

Our promoters have different affinities to the T7 RNA polymerase and the LacI repressor and therefore have different measurable properties. (igem.org)

The functions currently known for members of the KRAB-containing protein family include transcriptional repression of RNA polymerase I, II and III promoters, binding and splicing of RNA, and control of nucleolus function. (embl.de)
A sequence of 45 amino acids in the KRAB A subdomain has been shown to be necessary and sufficient for transcriptional repression. (embl.de)
Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins. (embl.de)
DNA methylation in promoters is closely linked to downstream gene repression. (biomedcentral.com)
QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
Oncogenic antigen- 519) as specific depletion of FASN, which itself similarly suppresses HER2 overexpression by inducing PEA3-dependent repression of HER2 gene promoter, strongly antagonizes the inhibitory effects of OA on HER2 gene promoter activity. (eurekaselect.com)

Genetic studies have exhibited multiple HLA- em DRB1 /em alleles encoding a conserved sequence at amino acid positions 70C74 are associated with susceptibility and severity of RA. (exposed-skin-care.net)
Although the concept of 'genetic programming' was emerging, the conclusion that genetic information is transacted primarily by proteins became entrenched, a reflection of the mechanical zeitgeist of the age, and assumed to be true both for bacteria and developmentally complex plants and animals. (taylorfrancis.com)
A codon is a DNA or RNA sequence of three nucleotides (a trinucleotide) that forms a unit of genetic information encoding a particular amino acid. (genome.gov)
Co-transcribed proteins are expressed proportionally to one another, the proportionality being dependent on their genetic context and RBS sequence. (github.io)
The polymerase then traverses the genetic material, and continues to yield RNA until it identifies a gene's transcription termination sequence, stopping the process. (jove.com)
This high-resolution snapshot of genetic profiles of 1710 GC sequences, through a comparison with 2018 data (1479 GC sequences) within the sentinel system, highlights change in proportions and expansion of select GC strains and the associated genetic mechanisms of resistance. (cdc.gov)
The four nucleotides of DNA are adenine, cytosine, guanine and thymine (A, C, G & T). The sequence of these four nucleotides within a DNA strand can occur in any order, and that sequence makes up the genetic code. (entandaudiologynews.com)
The genetic code of DNA determines what proteins can be produced by a cell, as well as the rate and time of protein production. (entandaudiologynews.com)

Promoters are upstream sequences that regulate transcription. (igem.org)

Since this RNA will be used to generate proteins, exons are denoted as coding regions, whereas introns are another example of non-coding material. (jove.com)
However, these intergenic regions carry important elements that regulate gene activity, for instance, the promoter where transcription starts, and enhancers and silencers that fine-tune gene expression. (jove.com)
Most peaks, clearly enriched for CArG-box sequences, are present in upstream promoter regions close to the transcriptional start site, though some are also found in introns and 3′-UTR regions. (frontiersin.org)
This control is also mediated by the sequence of the DNA in these noncoding regions. (entandaudiologynews.com)

Mutations in Ras proteins occur in around a third of all cancers, so a better understanding of their dynamics could help with future drug discovery. (elifesciences.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

Both the mSin3a and Mi-2/NuRD co-repressor complexes are large multi-component complexes in which not all of the components and their functions have been identified [ 2 , 6 ]. (biomedcentral.com)
The impact of oxidative stress on key components of the co-repressor complexes have only just started to be explored, with the very recent publication highlighting the impact of oxidative stress driven protein kinase-CK2 activation on co-repressor activity and HDAC2 function [ 20 ]. (biomedcentral.com)

ii)Frameshift mutation occurs where addition/insertion or deletion of one or two bases changes the reading frame from the site of mutation, resulting in a protein with a different set of amino acids. (infinitylearn.com)
Furthermore, mutation analyses of Fpr1 indicated that for transcriptional function on RPG promoters, Fpr1 requires its N-terminal domain and the binding surface for rapamycin, but not peptidyl-prolyl isomerase activity. (prolekarniky.cz)

The lactose utilization (lac) operon of Escherichia coli was shown to specify three enzymes and a regulatory locus, initially posited to express an RNA, but subsequently found to encode a DNA-binding repressor protein that is displaced upon lactose binding. (taylorfrancis.com)
The LacZ protein codes for an enzyme called β-galactosidase, which is an essential part of the metabolism of lactose. (presenternet.com)

The original BOB' sequence is changed by the integration to B-O-P'-phage DNA-P-O-B'. The phage DNA is now part of the host's genome. (wikidoc.org)
The repressor found in the phage lambda is a notable example of the level of control possible over gene expression by a very simple system. (wikidoc.org)
The lambda phage will remain in the lysogenic state if cI proteins predominate, but will be transformed into the lytic cycle if cro proteins predominate. (wikidoc.org)

Promoter - a nucleotide sequence that enables a gene to be transcribed. (presenternet.com)
1G/A:guanine/adenosine found on the first translated nucleotide following cloning into the H1 promoter. (berterolab.com)
The sequence of the four nucleotide bases encodes DNA's information. (genome.gov)
Each then forms the template upon which DNA polymerase syntheses a new double helix, with complementary nucleotide pairs ensuring faithful replication of the original DNA sequence. (entandaudiologynews.com)

KRAB (Kruppel-associated box) is an evolutionarily conserved protein domain found N-terminally with respect to the zinc finger repeats that encodes the DNA binding domain. (embl.de)
A major challenge in interpreting genome sequences is understanding how the genome encodes the information that specifies when and where a gene will be expressed. (sdbonline.org)

Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
By recruiting SWI/SNF chromatin remodellers to IκB-ζ, transcriptional coactivator for NF-κB, the conserved nuclear protein Akirin2 stimulates pro-inflammatory gene promoters in mouse macrophages during innate immune responses to viral or bacterial infection. (cnrs.fr)

Since the structural amount is binding, assistive introns around the physiological protein can be the method either to the lower or the longitudinal non-ignorable sets of joint model. (scoutconnection.com)
In bacteria and archaea , structural proteins with related functions are usually encoded together within the genome in a block called an operon and are transcribed together under the control of a single promoter , resulting in the formation of a polycistronic transcript ( Figure 12.30 ). (pressbooks.pub)

Standard techniques like cell synchronization by serum starvation, flow cytometry, N-terminal or C-terminal FLAG epitope-tagged protein expression, immunofluorescence confocal microscopy, dual luciferase assay for promoter evaluation, and chromatin immunoprecipitation assay were employed during this study. (biomedcentral.com)

The full sequence-verified ORF is natively expressed under an optionally inducible suCMV promoter. (nucleusbiotech.com)

Proteins were shown to be linear polymers of amino acids and the first three-dimensional structures of proteins solved. (taylorfrancis.com)
The order of nucleotides in the DNA was hypothesized and confirmed to instruct the sequence of amino acids in proteins via a temporary ('messenger') RNA copy of the gene and adaptor 'transfer RNAs' that connected the two. (taylorfrancis.com)
An amino acid is the fundamental molecule that serves as the building block for proteins. (genome.gov)
A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)
Insertion or deletion of three of its multiples of bases do not alter the reading frame but one/more amino acids are coded in the protein translated. (infinitylearn.com)
Proteins consist of strings of amino acids, and the identity of each amino acid is encoded by a sequence of three DNA nucleotides known as a codon. (entandaudiologynews.com)

Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. (wikipedia.org)
Importantly, both the diffusion and trafficking properties of KRas G12D remain invariant over a broad range of protein expression levels. (elifesciences.org)
HMGCS2 protein expression is down-regulated preferentially in moderately and poorly differentiated carcinomas. (aacrjournals.org)
Protein expression and function of organic anion transporters in short-term and long-term cultures of Huh7 human hepatoma cells. (cancerindex.org)
Surprisingly, in vivo a similar increase in expression from the above promoters was observed for the mutant deficient in GltR regardless of the presence of effectors. (nih.gov)
The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. (qiagen.com)
The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)
Expression of the TEA/ATTS DNA binding domain that acts a dominant negative repressor of TEAD factors in C2C12 myoblasts inhibits their differentiation, while selective shRNA knockdown of TEAD4 results in abnormal differentiation characterised by the formation of shortened myotubes. (nih.gov)
Subcellular location of ZAR2 and its expression from the reverse promoter of the BRCA2 gene are stringently regulated in a cell cycle dependent manner. (biomedcentral.com)
This protein belongs to the rel proto-oncogene and NF-KB transcriptional factor family and acts by controlling zygotic gene expression. (silverchair.com)
Whereas regulating gene expression in multicellular organisms allows for cellular differentiation, in single-celled organisms like prokaryotes, it primarily ensures that a cell's resources are not wasted making proteins that the cell does not need at that time. (pressbooks.pub)
Fourth, HER2 gene promoter bearing a PEA3 site-mutated sequence cannot be negatively regulated by OA, while treatment with OA fails to repress the expression of a human full-length HER2 cDNA controlled by a SV40 viral promoter. (eurekaselect.com)
Wild type, PI3Kγ knock-out (PI3Kγ -/- ) and PI3K kinase dead knock-in (PI3Kδ D910/A910 ) transgenic mice were exposed to cigarette smoke for 3 days and the expression levels of the co-repressor complex components HDAC-2, mSin3a, Mi-2α and Mi-2β and HDAC-2 activity in the lungs were assessed. (biomedcentral.com)
Both mSin3a and Mi-2α protein expression was reduced in smoke-exposed mice. (biomedcentral.com)
Budesonide alone protected mSin3a protein expression with no additional effect seen with abrogation of PI3Kγ/δ activity, however Mi-2α, but not Mi-2β, expression was protected in both PI3Kδ D910/A910 and PI3Kγ -/- budesonide-treated smoke-exposed mice. (biomedcentral.com)
The restoration of glucocorticoid function coincided with the protection of both HDAC activity and mSin3a and Mi-2α protein expression. (biomedcentral.com)
Cigarette smoke exposure induced glucocorticoid insensitivity and alters co-repressor activity and expression which is prevented by blockade of PI3K signaling with glucocorticoid treatment. (biomedcentral.com)
As a result, for each protein-reporter pair, the expression rates are proportional and autodegradation rates can be treated as identical in the model. (github.io)
Among them a promoter, exons, introns, and regulatory elements, which together help to determine protein expression in a cell. (jove.com)
With this system, high levels of heat-induced transgene expression were observed compared to that from the HSP promoter alone without the transactivator. (elsevierpure.com)
In a hmo1 Δ (high mobility group family 1-deleted) yeast strain, deletion of FPR1 induced severe growth defects, which could be alleviated by increasing the copy number of RPL25 (ribosome protein of the large subunit 25), suggesting that RPL25 expression was affected in hmo1 Δ fpr1 Δ cells. (prolekarniky.cz)
However, the cis-regulatory sequence controlling this posterior expression pattern has not been precisely identified. (sdbonline.org)

A 1.1-kb fragment containing this cluster was placed in a reporter construct containing the eve minimal promoter fused to a lacZ reporter gene. (sdbonline.org)

It is a nuclear protein that is largely chromatin-bound and has a strong preference for binding to methylated DNA sequences in vivo . (portlandpress.com)

The 﫿rst step in generating OPTiKD hPSCs consists in obtaining oligonucleotides to be used to clone the shRNA sequence in the targeting vector. (berterolab.com)

A new system for naming ribosomal proteins. (prolekarniky.cz)

An allele is one of two or more versions of DNA sequence (a single base or a segment of bases) at a given genomic location. (genome.gov)

c-Myc transrepression is mediated by blockade of the transactivating activity of Miz-1, which occurs mainly through a Sp1-binding site in the proximal promoter of the gene. (aacrjournals.org)
The KRAB domain functions as a transcriptional repressor when tethered to the template DNA by a DNA-binding domain. (embl.de)
Fpr1 (FK506-sensitive proline rotamase 1 ) , a protein of the FKBP12 (FK506-binding protein 12 kDa) family in Saccharomyces cerevisiae , is a primary target for the immunosuppressive agents FK506 and rapamycin. (prolekarniky.cz)
Intriguingly, Fpr1 promotes the binding of Fhl1/Ifh1 (forkhead-like 1/interacts with forkhead 1), two key regulators of RPG transcription, to certain RPG promoters independently of and/or cooperatively with Hmo1. (prolekarniky.cz)
Additionally, four common transcription factors: FadR, TFEb, CRISPR loci binding protein, and HTH family protein were consistently overexpressed across all stress conditions, indicating their significant role in managing stress. (jcbose.ac.in)
The binding sequences for each factor were aligned by using the motif-assembly program, and the binding specificities of each factor were modeled with position weight matrices (PWMs). (sdbonline.org)
PWMs are a useful way to represent binding specificities and provide a statistical framework for searching for novel instances of the motif in genome sequences (Berman, 2002). (sdbonline.org)
C promoter binding factor/ Suppressor of Hairless/ lag- 1). (bvsalud.org)

Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. (prolekarniky.cz)

HDACs function by deacetylating of key components of the transcriptional machinery including the core histone proteins resulting in their in re-association with the DNA, thus presenting a transcriptionally closed conformation [ 1 , 16 ]. (biomedcentral.com)

An average promoter methylation is usually used in studies, whereas recent results suggested that methylation of individual cytosines can also be important. (biomedcentral.com)

Identical LVA-ssrA degradation tags were appended to the coding sequences of the synthase, repressor, and reporter proteins to promote equal degradation rates, represented in the model as \(\rho\) with subscripts indicating the associated model species. (github.io)

BRCA2 gene promoter has bi-directional activity, expressing BRCA2 and a novel C4-type zinc finger containing transcription factor ZAR2. (biomedcentral.com)
Hb, Kr, and Kni are zinc-finger gap proteins that act primarily as repressors in specific embryonic domains. (sdbonline.org)

Here, we show that Akirin2, an evolutionarily conserved nuclear protein, bridges NF-κB and the chromatin remodeling SWI/SNF complex by interacting with BRG1-Associated Factor 60 (BAF60) proteins as well as IκB-ζ, which forms a complex with the NF-κB p50 subunit. (cnrs.fr)

The newly minted 'Central Dogma' of molecular biology asserted that 'DNA makes RNA makes proteins', with RNA tacitly but firmly relegated to an ephemeral intermediate. (taylorfrancis.com)

Transcription factor AP-2 beta also known as AP2-beta is a protein that in humans is encoded by the TFAP2B gene. (wikipedia.org)

The third class of thermosensing is based on the temperature-dependent conformation of specific RNA sequences, which are termed RNA-thermometer (RNAT). (springer.com)

A Blasticidin-RFP fusion dual marker under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of transduced cells. (nucleusbiotech.com)

Due to the inherent diversity in the underlying mechanisms, protein-based thermosensors affect different cellular processes such as transcription, translation, protein stability, signal transduction as well as proteolytic processes. (springer.com)

A key mechanism appears to be the following: KRAB-AFPs tethered to DNA recruit, via their KRAB domain, the repressor KAP1 (KRAB-associated protein-1, also known as transcription intermediary factor 1 beta, KRAB-A interacting protein and tripartite motif protein 28). (embl.de)

While studying the activity of BRCA2 gene promoter in breast cancer cells, we discovered that this promoter has bi-directional activity and the product of the reverse activity (a ZAR1-like protein, we named ZAR2) silences the forward promoter at the G0/G1 phase of the cell. (biomedcentral.com)
This promoter is 8-20 fold more active in the reverse orientation than in the forward orientation when the cells are in the non-dividing stage (G0/G1). (biomedcentral.com)
When the cells are in the dividing state (S/G 2 ), the forward activity of the promoter is 5-8 folds higher than the reverse activity. (biomedcentral.com)
We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
Second, OA exposure specifically represses the transcriptional activity of the human HER2 gene promoter in tumor-derived cell lines naturally exhibiting HER2 gene amplification and p185Her-2/neu protein overexpression but not in cancer cells expressing physiological levels of HER2. (eurekaselect.com)
Third, OA treatment induces the upregulation of the Ets protein PEA3 (a transcriptional repressor of the HER2 gene promoter) solely in cancer cells naturally displaying HER2 gene amplification. (eurekaselect.com)
In the first phase, sender cells grow and accumulate synthase protein. (github.io)
This propagation ends at the third phase, when cell growth and protein production slow down such that the amplifier cells cannot produce enough synthase protein to sustain signal transmission. (github.io)
Protein quantity is also diminished through dilution as cells grow and divide. (github.io)

The KRAB domain is a protein-protein interaction module which represses transcription through recruiting corepressors. (embl.de)

The key co-repressor complex components HDAC-2, Mi-2α/β and mSin3a are all critical to the regulation of gene transcription. (biomedcentral.com)
Furthermore, Akirin2 and IκB-ζ recruitment to the Il6 promoter depend upon the presence of IκB-ζ and Akirin2, respectively, for regulation of chromatin remodeling. (cnrs.fr)

This study characterized high-quality whole-genome sequences of a sentinel, surveillance-based collection of 1710 Neisseria gonorrhoeae (GC) isolates from 2019 collected in the USA as part of the Gonococcal Isolate Surveillance Project (GISP). (cdc.gov)
The 1710 isolates represented 164 multilocus sequence types and 21 predominant phylogenetic clades. (cdc.gov)

While the gene circuit parameters inferred from liquid-culture experiments are maintained, microscope data are used to determine parameters governing nutrient-dependent growth and protein production. (github.io)

The times below were that site-specific selection of self-esteemproblems has on the survival of the & of splines so that higher gene strains and larger promoter saponins show more adjacent biomarkers. (scoutconnection.com)

Temperature has a direct effect on fundamental biological systems, including enzyme activity and correct folding of proteins. (springer.com)

Anatomy11

Organisms14

Diseases1

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes104

Disciplines and Occupations1

Information Science5

Genes28

Binds7

Bind16

Synthesis9

Tetracycline repressor2

Leading to transcriptional repression2

MRNA10

Activator5

LacI1

Repression6

Genetic8

Upstream sequences1

Regions4

Mutations2

Complexes2

Mutation2

Lactose2

Phage3

Nucleotide4

Encodes2

Bacterial2

Structural2

Chromatin immunoprecipitation1

Inducible1

Amino6

Expression25

Minimal promoter1

Vivo1

Consists1

Ribosomal1

Genomic1

Binding8

Mammalian1

Histone1

Methylation1

Reporter1

Zinc finger2

Evolutionarily conserved1

Molecular biology1

Transcription factor1

Temperature-dependent1

Fluorescence1

Signal Transduction1

Factor1

Cells9

Interaction1

Regulation2

Isolates2

Dependent1

Strains1

Enzyme1