Probes biological assay. Medical search. Frequent questions

BD Biosciences also offers a range of tools for caspase activity assays from individual fluorogenic peptide substrates and inhibitors, to kits, to ready-to-use assay plates. (bdbiosciences.com)
In biological activity assays, this can cause problems with measuring the activity of a specific protein or pathway. (nih.gov)

In the study published last week in PLoS Neglected Tropical Diseases , Shijman and his collaborators described a multiplex, TaqMan probe-based, real-time PCR assay for the six DTUs. (genomeweb.com)
The Poxvirus and Rabies Branch (PRB) developed this document as a test procedure for the Monkeypox virus generic real-time PCR assay. (cdc.gov)

QuantiNova LNA Probe PCR Focus Panels enable quick and reliable gene expression analysis of over 150 pathways each for human, mouse or rat using hydrolysis probe-based qPCR. (qiagen.com)
This review provides an insight into real-time quantitative PCR (qPCR) assays today. (ddw-online.com)
and progress in applying qPCR assays to novel assay application areas. (ddw-online.com)
To complement that review, HTStec recently undertook a qPCR end user survey to understand current practices and preferences in qPCR assays and instruments, and to identify future user requirements (2). (ddw-online.com)
In this article we highlight some of the main findings of the resulting HTStec qPCR Assay Trends market report. (ddw-online.com)
It also provides an insight into how qPCR assays are typically undertaken today in many labs and what influences the choice in purchasing qPCR mastermixes and thermal cycling instruments. (ddw-online.com)
The alternative is purchasing qPCR bulk reagents separately, where the end user would optimise and validate each assay in their own lab. (ddw-online.com)
Here, we describe a protocol to quantify microRNA editing isoforms using two-tailed RT-qPCR, with either SYBR Green or hydrolysis probes. (lu.se)

Finally, he recognized that the positron-emitting forms of oxygen, nitrogen, carbon and fluorine provide the tools to "label" biochemical molecules for their use as probes, to non-invasive image biological processes in living individuals. (wikipedia.org)
Phelps subsequently developed an array of biological assay techniques for PET-based measurements of hemodynamic, biochemical, and biological processes in the brain, heart, and tumors. (wikipedia.org)
Phelps also conceptualized the miniaturization, automation, and integration of cyclotron technology and biochemical synthesizers necessary for the synthesis of positron-labeled probes into a single, PC-controlled device for producing positron-labeled compounds for research and clinical care. (wikipedia.org)
Potential off-targets within the S 35 (1 μM) fraction as well as PIKfyve were tested using a NanoBRET target engagement assay and via biochemical enzymatic assays where an assay was available. (thesgc.org)
Techniques used span from synthetic chemistry to protein expression and biochemical assays. (hofstra.edu)

Topics covered will include high-throughput assay development, screening, chemical library creation, high-throughput chemistry, affinity purification of target proteins and target validation, protein microarrays, molecular evolution, protein engineering and synthetic biology. (columbia.edu)

Khan NA, Jarroll EL, Paget TA (2001) Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. (springer.com)
Enzyme-linked immunosorbent assay and polymerase chain reaction testing were performed. (who.int)
Several methods have been devised recently for very sensitive detection of Variola virus DNA and to distinguish this DNA from that of other orthopoxviruses, the most promising being analysis by polymerase chain reaction (PCR) of restriction fragment length polymorphisms, multiplex PCR and real-time PCR with fluorigenic probes. (who.int)

In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluorescent ligation assay. (asm.org)
Recently, a fluorescent based ChE assay kit was marketed for measurement of the purified enzyme (A12217, Molecular Probes, Eugene, OR, USA ( Zhou et al 2000). (cdc.gov)
The objective of this study was to evaluate this fluorescent assay as a tool to measure ChE activities in biological specimens collected from humans and wildlife. (cdc.gov)
probes, molecular reporters and fluorescent indicators for structural or functional imaging or microscopy/nanoscopy. (nih.gov)

It is said to provide high sensitivity (0.002 units/ml of purified AChE from electric eel, as reported by Molecular Probes) without hemoglobin interference. (cdc.gov)
I showed that molecular probes using them as reduction sensors can be mostly processed by thioredoxins while being stable against reduction by GSH. (uni-muenchen.de)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
With the help of various forms of mass spectrometry, synchrotron radiation, protein production & labelling, and bioinformatics, our facilities provide the opportunity to study protein structure and dynamics, molecular probes and drug design. (lu.se)

The conjugation was evaluated by fluorescence correlation spectroscopy (FCS) and fluorescence microplate assay (FMA). (bvsalud.org)

Selective small molecule probes for the hypoxia inducible factor (HIF) prolyl hydroxylases. (ox.ac.uk)
Dr. Jaremko's research interests are in the investigation of enzymes involved in natural product pathways, including fatty acids and non-ribosomal peptides (pharmaceuticals), using small molecule probes and inhibitors. (hofstra.edu)

Second, using the principle of coincidence detection, he configured a circumferential array of detectors and associated electronics and a mathematical algorithm for forming three-dimensional tomographic images of biological probes of the living human body. (wikipedia.org)
Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
Twenty years of LNA design experience has enabled us to optimize our sophisticated LNA design algorithm, which incorporates over 50 different parameters to guarantee the most optimal assay for successful target detection. (qiagen.com)
The increased sensitivity ensures excellent amplification efficiencies down to 1 RNA molecule, making it easier for you to detect low-abundance targets such as lncRNAs from less starting material (see figure QuantiNova LNA Probe PCR Assays provide accurate, sensitive and linear quantification of targets over a wide dynamic range and figure QuantiNova LNA Probe PCR Assays enable both high-expression and low-expression detection of mRNA. The PCR performance of QuantiNova LNA PCR Assays was evaluated using a commercially available DNA template (gBlock). DNA templates were serially diluted from 1x10 9 to 10 copies and quantified with QuantiNova LNA PCR Assays using a Bio-Rad CFX96 or Bio-Rad CFX384 instrument. The QuantiNova LNA PCR Assays exhibited high linear range combined with outstanding sensitivity and near optimal efficiency. This high linearity along the entire input range is essential to prevent biases in calculations of relative expression. "> QuantiNova LNA Probe PCR Assays enable both high-expression and low-expression detection of mRNA ). (qiagen.com)
While the Ellman assay is simple and inexpensive, the absorbance peak of hemoglobin (415nm) can interfere with detection of its reaction end-product, 2-nitro-5-benzoic acid (405nm). (cdc.gov)
The inherent redundancy of each probe type on the array, because each microsphere type has many replicates, provides additional confidence in detection and enhances the signal-to-noise ratio. (cdc.gov)
To ensure sensitive detection of BWAs with high accuracy, the performance of this multiplexed, high-density array was assessed by examining the detection limits and specificity of probes with Cy3-labeled synthetic targets (the synthetic target is a 50-mer oligonucleotide complementary to the probe). (cdc.gov)
While the probes were selected to avoid the formation of secondary structures, such structures were not completely eliminated and probably affected the hybridization efficiency, resulting in higher detection limits for some probes on the array. (cdc.gov)
Full validation of the above assays, including intra-assay/inter-assay variability, spike recovery, dilution linearity, and limit of detection, is not usually reported. (jneurosci.org)
If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327) . (abcam.com)
Most recently, GeneXpert MTB/RIF (Cepheid) advanced nucleic acid-based detection by integrating PCR and a highly sensitive molecular-beacon assay into a single, automated system. (cdc.gov)
The entire assay (hybridization plus visual detection) was performed in less than 10 min. (upm.edu.my)
We believe the assay can be applied for species assignment in food analysis, mismatch detection in genetic screening and homology studies between closely related species. (upm.edu.my)
Here, we developed a nanoPCR assay for the rapid detection of brain-eating amoebae using various nanoparticles. (springer.com)
Using nanoparticle-assisted PCR, the results revealed that graphene oxide, copper oxide and alumina nanoparticles significantly enhanced PCR efficiency in the detection of pathogenic free-living amoebae using genus-specific probes. (springer.com)
The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Such probes may be useful to investigate the biological role of CB2 receptors in cellular assays. (nih.gov)
They used synchrotron X-ray fluorescence microscopy (XFM) to probe the cellular uptake and distribution of TRIP and an iodo-derivative I-TRIP by looking at elemental signals. (rsc.org)
Proteins are the primary class of biological macromolecules and serve to carry out most cellular functions. (columbia.edu)
However, the precise cellular roles of the central oxidoreductase systems, including TrxR and Trx, remain inaccessible due to the lack of probes to selectively measure turnover by either of these proteins. (uni-muenchen.de)
By comparing a novel 1,2 dithiolane-based probe to the state-of-the-art probes, including commercial TrxR sensors, by screening a conclusive assay panel of cellular TrxR modulations, I clarified that 1,2 dithiolanes are not selective substrates for TrxR in biological settings (Nat Commun 2022). (uni-muenchen.de)

LNA enhancement enables T m normalization across the panel and gives the primers and probes higher binding affinity, dramatically increasing assay sensitivity and specificity. (qiagen.com)

Now, scientists in Argentina have developed a multiplex PCR assay that may simplify the process, and have demonstrated its potential by typing a large set of international samples. (genomeweb.com)
Several common genotyping techniques include restriction fragment length polymorphism (RFLP), terminal restriction fragment length polymorphism (t-RFLP),[2] amplified fragment length polymorphism (AFLP),[3] and multiplex ligation-dependent probe amplification (MLPA). (biostars.org)
Hence, Luminex ELISAs can multiplex upto 100 analytes in a single assay well. (magazinexu.com)
Thus, through 96-well or 364-well plates, Luminex ELISA multiplex assays provide higher throughput and density. (magazinexu.com)
The assay can multiplex upto 100 tests in a single run and run upto 1000 study samples every day. (magazinexu.com)

The goal of the MLP is to screen compounds in the Small Molecule Repository in target-based and phenotypic assays to identify and subsequently optimize small molecule s as research probes. (nih.gov)

This assay detects DNA at varying concentrations, providing a qualitative result of either positive, negative, or inconclusive in the identification of Monkeypox virus infections. (cdc.gov)
The line probe assay, for instance, colorimetrically detects multi-drug resistant (MDR) strains from PCR-amplified products MTB DNA. (cdc.gov)

Evaluation of our ever-growing collection of SuFExable compounds toward various biological assays unexpectedly revealed a selective and covalent hNE inhibitor: benzene-1,2-disulfonyl fluoride. (nih.gov)

The most widely used is a colorimetric assay developed by Ellman et al (1961). (cdc.gov)
Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. (abcam.com)
This product is manufactured by BioVision, an Abcam company and was previously called K262 Nitric Oxide Colorimetric Assay Kit. (abcam.com)

The lower limit of reliable quantification of the assay was 0.18 pg/ml and the antibody pairs recognized Aβ multimers comprised of either synthetic standards, or endogenous oligomers isolated from confirmed human AD and healthy control brain. (jneurosci.org)
The chemoproteomic toolkit is anchored by liquid chromatography-tandem mass spectrometry (LC-MS/MS or LC-MS) based quantitative proteomics , which allows for the near complete identification and relative quantification of complex proteome s in biological samples. (explained.today)
To optimally utilize the biological and clinical information that is contained in microRNA expression levels, tools for their accurate and cost-effective quantification are needed. (lu.se)

In concept, Luminex assays are numerous ELISA assays on a bead. (magazinexu.com)
Luminex ELISA assays produce accurate results similar to traditional ELISA assays. (magazinexu.com)
Besides, these results are as accurate and reliable as conventional ELISA assays. (magazinexu.com)
Patients were divided into 3 age morbidity and mortality in patients with generation enzyme-linked immuno- groups (Table 1) but no significant end-stage renal disease (ESRD) treated sorbent assay (ELISA) kits (Diagnostic relationship between HCV genotype by dialysis or transplantation [1-3]. (who.int)

In this review we summarize the current state of the art in detailing the three main categories of quadruplex-binding macrocycles described so far (telomestatin-like polyheteroarenes, porphyrins and derivatives, polyammonium cyclophanes), and in addressing both synthetic issues and biological aspects. (hindawi.com)
To evaluate probe specificity, arrays containing beads with only a single probe type were exposed sequentially to the synthetic complementary target as well as to all noncomplementary targets. (cdc.gov)
Synthetic derivatization of the initial hit led to a more potent agent, 2-(fluorosulfonyl)phenyl fluorosulfate with IC 50 0.24 μM and greater than 833-fold selectivity over the homologous neutrophil serine protease, cathepsin G. The optimized, yet simple benzenoid probe only modified active hNE and not its denatured form. (nih.gov)
The individual synthetic tetrapeptide substrates, together with the caspase inhibitors and active caspase enzymes, offer flexibility in the experimental design of a caspase activity assay. (bdbiosciences.com)
We also give guidelines for primer design and for the evaluation of assays using synthetic oligonucleotides. (lu.se)

We identify suitable probe compounds for use in studies on the functional effects of PHD inhibition in cells and in animals. (ox.ac.uk)

You've identified a chemical that you'd like to use in your research as a chemical probe for a receptor or an enzyme inhibitor. (nih.gov)
The negative control was sent to SignalChem for testing in enzyme assay for PIKfyve. (thesgc.org)

The high binding affinity of LNA bases increases the flexibility of primer and probe placement on the transcript, so we are able to use intelligent positioning to design assays for otherwise difficult-to-analyze targets. (qiagen.com)
BET bromodomain ligands: Probing the WPF shelf to improve BRD4 bromodomain affinity and metabolic stability. (ox.ac.uk)

These probes include receptors, antibodies, and peptides. (magazinexu.com)
Luminex assays can be conjugated with receptors, antibodies, and peptides, and thus their applications can be extended to protein expression and gene expression profiling, HLA testing, and much more. (magazinexu.com)

Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
We will discuss the structure and function of both proteins and small molecules in biological systems. (columbia.edu)

Each primer-probe set delivers the highest specificity and efficiency for the most reliable and accurate results. (qiagen.com)
All probe sequences were designed with software by Illumina, Inc. (San Diego, CA, USA), and the specificity of the probe sequences was checked against GenBank by using BLAST to have minimal cross-hybridization potential. (cdc.gov)
The specificity of the assay is confirmed by a panel of clinically relevant non-MTB bacteria, and the clinical utility is demonstrated by the measurements in MTB-positive patient specimens. (cdc.gov)

The course is intended to provide a foundation needed for advanced chemical biology research, i.e. the creation and use of chemical probes of biological processes and macromolecular function. (columbia.edu)
High-throughput sequencing has helped to transform our study of biological organisms and processes. (umd.edu)
Management and prognosis of disease requires the accurate determination of specific biomarkers indicative of normal or disease-related biological processes or responses to therapy. (mdpi.com)
Countless biological processes allow cells to develop, survive, and proliferate. (uni-muenchen.de)

Serine proteases play crucial biological roles and have their activity controlled by inhibitors, such as the EcTI, a serine protease inhibitor purified from Enterolobium contortisiliquum seeds, which has anticancer activity. (bvsalud.org)

We sought to develop a robust assay to quantify oligomers from CSF. (jneurosci.org)
Instead, aiming for more stable ring systems and thus more robust redox probes, during this work, I developed bicyclic 6 membered disulfides (piperidine fused 1,2 dithianes) with remarkably low reduction potentials. (uni-muenchen.de)
The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. (megazyme.com)

We simultaneously identified multiple biological warfare agents in environmental samples by looking at specific probe responses after hybridization and response patterns of the multiplexed array. (cdc.gov)
Also known as a genotypic assay, techniques include PCR, DNA fragment analysis, allele specific oligonucleotide (ASO) probes, DNA sequencing, and nucleic acid hybridization to DNA microarrays or beads. (biostars.org)
Hybridization of a 27-nucleotide probe to swine mitochondrial DNA identified them in pork-venison, pork-shad and venison-shad binary admixtures, eliminating the need of PCR amplification. (upm.edu.my)

The Vanderbilt Chemical Synthesis and High-throughput Analytics Shared Resource (CSHTASR) provides state-of-the-art capabilities for the Vanderbilt-Ingram Cancer Center researchers, and works to harness the power of chemistry and assay technologies to support research requiring the application or discovery of chemical tools to answer biological questions. (vicc.org)

Various assays and biological studies showed evidence of comparable cytotoxicity and mechanism, demonstrating that altering the substitution of the isonitrile ligand doesn't significantly impact the bioactivity of the complex. (rsc.org)

Until now the standard procedure to validate antibodies for IP couples the IP assay to immunoblotting (IP/IB), a procedure that is challenging to scale to high-throughput analysis [ 10 ]. (biomedcentral.com)

We also offer Flexible Panels, which are semi-customizable from the assays in our Focus Panels, as well as Custom Panels with your choice of predesigned and/or custom assays. (qiagen.com)
Luminex assays offer custom panels and probes. (magazinexu.com)

From a library of indolyl pyrimidinamines, we identified a highly potent and cell-active chemical probe ( SGC-PIKFYVE-1 ) that inhibits phosphatidylinositol-3-phosphate 5-kinase (PIKfyve). (thesgc.org)
Our PIKfyve chemical probe disrupts multiple phases of the β-coronavirus lifecycle: viral replication and viral entry. (thesgc.org)
Our chemical probe set can be used by the community to further characterize the role of PIKfyve in virology and explore its other roles as well. (thesgc.org)
Several targets of high profile drug s have been identified using chemoproteomics, and the continued improvement of mass spectrometer sensitivity and chemical probe technology indicates that chemoproteomics will play a large role in future drug discovery . (explained.today)

Luminex assays can effortlessly evaluate and measure critical biological elements in study samples. (magazinexu.com)

We developed a fiber-optic, microsphere-based, high-density array composed of 18 species-specific probe microsensors to identify biological warfare agents. (cdc.gov)
A multiplexed array was fabricated by distributing up to 18 different microsphere sensors into the optical fiber array with species-specific 50-mer DNA probes corresponding to 6 BWAs of interest ( Bacillus anthracis , Yersinia pestis , Francisella tularensis , Brucella melitensis , Clostridium botulinum , and vaccinia virus) and 1 BWA simulant, Bacillus thuringiensis kurstaki , with each organism represented by at least 2 probe sequences ( Table A1 ). (cdc.gov)
Because the assembly process for the arrays is random, each bead type is encoded with an identifying optical bar code ( 11 , 12 ) created by entrapping dyes in various combinations within microspheres, to differentiate microsphere probe types from one another. (cdc.gov)

Tests or protocols may include hazardous reagents or biological agents. (cdc.gov)

Topics covered include introductions to biomolecular structure, physico-chemical tools used to probe biomolecular structure and dynamics, intermolecular forces that govern these structures and dynamics, and experimental and computational approaches for studies of biomolecular folding and function. (columbia.edu)
These studies use a range of techniques, from computational modelling, to biological assays, to Nuclear Magnetic Resonance Spectroscopy (NMR). (hofstra.edu)
Of interest are development of computational and mathematical algorithms and tools, modeling techniques and approaches for understanding the complexity of biological systems, and utilization of big datasets and data science methods for model construction. (nih.gov)

Un test immuno-enzymatique et une amplification en chaîne par polymérase ont été réalisés. (who.int)

The researchers will now use this assay for molecular epidemiology, and have published the primer and probe sequences "so everybody can reproduce the test for their own interests," Shijman said. (genomeweb.com)
Researchers employ several techniques for evaluating cytokines in biological samples. (magazinexu.com)
Researchers can customize study probes on assay panels and develop custom Luminex assays for individual research needs. (magazinexu.com)

NPs are very important because they have superior physico-chemical and biological characteristics compared to the bulk material. (springeropen.com)

They can analyze a wide range of biological samples such as saliva, sputum, urine, cell and tissue lysate, plasma, and serum, making them a crucial screening modality. (magazinexu.com)

Employing multiple probes for each BWA reduces the potential for both false-negative and false-positive results in environmental samples. (cdc.gov)
They then used the new assay to type 307 different biological samples and 39 cultured stocks of T. cruzi . (genomeweb.com)
Biological samples are always limited. (magazinexu.com)
Luminex assays are indeed crucial in identifying and quantifying cytokines from study samples. (magazinexu.com)
However, such probes would allow measuring their effective reductive activity apart from expression levels in native systems, including in cells, animals, or patient samples. (uni-muenchen.de)
Thus the assay was applied to authenticate species both in PCR-amplified and non-amplified heterogeneous biological samples. (upm.edu.my)
The LOD (for genomic DNA) of the assay was 6 µg ml − 1 swine DNA in mixed meat samples. (upm.edu.my)
Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc. (megazyme.com)
Procedures for the determination of ammonia in biological samples are found in Table 7-1. (cdc.gov)
The amount of ammonia in collected blood, urine, saliva, or other biological fluid samples can be affected by several mechanisms that may lead to erroneous ammonia concentration determinations. (cdc.gov)

We will discuss approaches for discovering and optimizing chemical tools for measuring and perturbing biological systems. (columbia.edu)

SOR), hydrogen peroxide, and 2, 2-Diphenyl-2-picrylhydrazyl (DPPH) radical activity by a model (most biological y active) of the anticancer plant was also evaluated. (who.int)

Find or custom design the right target-specific assays and panels to research your biological targets of interest. (qiagen.com)
Ready-to-use plate containing research area- or pathway-focused assays for mRNA targets in 96- or 384-well format for any cycler. (qiagen.com)
At NCBI, the PubChem BioAssay database stores biological activity assay information that makes it possible to find experimentally measured targets for millions of chemicals. (nih.gov)

The CSHTASR provides a full range of services, from utilization of equipment and pilot assay development, to a full screen including hit validation/optimization. (vicc.org)

NICEATM and NC3Rs are partnering with the National Institute of Allergy and Infectious Diseases, the U.S. Army Combat Capabilities Development Command Chemical Biological Center, and NCATS to direct the MPS for COVID Research (MPSCoRe) working group. (nih.gov)
The immunoprecipitation assay (IP) is an important tool used in academic and industrial research labs for applications such as targeted protein purification, protein concentration, analysis of protein-protein interactions, identification/analysis of protein complexes and analysis of protein/DNA interactions using chromatin IP (ChIP). (biomedcentral.com)

BlockquoteGenotyping is the process of elucidating the genotype of an individual with a biological assay. (biostars.org)
Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays. (ox.ac.uk)
In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. (ox.ac.uk)

Additionally, the tunability and stable bioactivity of the Re complexes shows that they're amenable to study by a wide range of techniques that will allow for further mechanistic probing. (rsc.org)
The applicant's mentor, Dr. Brian M. Salzberg, is a widely respected expert in the field of optical probes and optical techniques and will act as a facilitator of these career goals. (grantome.com)
Biostatistics areas of interest include development of advanced statistical techniques and methodologies for design of biological experiments, collection and analysis of the data from those experiments and interpretation of, and inference from, the results. (nih.gov)

We modified our previous protocol of microsensor preparation ( 12 ) by using an increased concentration of cyanuric chloride solution in the probe activation step to achieve a more complete and efficient coupling of DNA probes to microspheres. (cdc.gov)
No indemnification for any loss, claim, damage, or liability is provided for the party receiving an assay or protocol. (cdc.gov)

details in Table A2 ) because some of the sequences for each probe pair overlapped, as probes were designed to be specific to the same target gene of the BWA. (cdc.gov)

With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)

Such multiagent analysis is difficult because it is usually complicated by interferences between assays and the large number of BWA probes. (cdc.gov)
However, Luminex assays have been increasingly employed in cytokine analysis. (magazinexu.com)
The Luminex assay offers both high-throughput and high-density sample analysis. (magazinexu.com)
The standard assay couples IP to immunoblotting (IP/IB), a procedure severely limited as it is not easily scaled for high-throughput analysis. (biomedcentral.com)

Likewise, metagenomic sequencing lets us probe natural environments to profile organismal diversity and to discover new strains and species that may be integral to the environment or process being studied. (umd.edu)
Density functional theory calculations, with additions that account for van der Waals forces, are used to probe the delicate electronic interactions at the surface-adsorbate interface and between species within solvents. (hofstra.edu)

Anatomy3

Organisms2

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Phenomena and Processes13

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Activity assays2

Real-time PCR2

QPCR8

Biochemical5

High-throughput assay1

Polymerase chain re3

Fluorescent4

Molecular Probes4

Microplate1

Small molecule probes2

Detection16

Cellular5

Primers1

Multiplex5

Phenotypic1

Detects2

Selective1

Colorimetric3

Quantification3

ELISA4

Synthetic5

Compounds1

Enzyme2

Affinity2

Receptors2

Proteins2

Specificity3

Processes4

Inhibitors1

Robust3

Hybridization3

Chemistry1

Cytotoxicity1

Antibodies1

Custom Panels2

Chemical probe4

Evaluate1

Microsphere3

Reagents1

Computational3

Amplification1

Researchers3

Physico-chemical1

Wide range of biological1

Samples10

Approaches1

Hydrogen1

Targets3

Utilization1

Research2

Genotype3

Techniques3

Protocol2

Target1

Heterogeneous1

Analysis4

Species2