• Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean (Canavalia ensiformis). (wikipedia.org)
  • ConA was the first lectin to be available on a commercial basis, and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities on the surface of various cells. (wikipedia.org)
  • It is also used to purify glycosylated macromolecules in lectin affinity chromatography, as well as to study immune regulation by various immune cells. (wikipedia.org)
  • Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
  • The liver injury was induced by injection of concanavalin A (con-A), a lectin derived from jack beans. (biomedcentral.com)
  • This "B"-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. (vectorlabs.com)
  • The A -rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α- N -acetylgalactosamine residues, while the B -rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. (vectorlabs.com)
  • Similar to phytohemagglutinin (PHA), it is a selective T cell mitogen relative to its effects on B cells. (wikipedia.org)
  • METHODS: Peripheral blood mononuclear cells (PBMC) obtained from untreated or IFN beta-1-treated patients with relapsing-remitting MS or from healthy donors (HD) and T cells were stimulated with concanavalin A, phytohemagglutinin, or antibody to CD3 in the presence of lovastatin, simvastatin, mevastatin, IFN beta-1b, or statins plus IFN beta-1b. (dr-niederwieser.at)
  • The effect of graded amounts of dietary lactalbumin (L) and casein (C) hydrolyzates on the immune responsiveness of C3H/HeN and DBA/2 strain mice has been investigated by measuring both the specific humoral immune response to sheep red blood cells (SRBC) and the nonspecific splenic cell responsiveness to phytohemagglutinin, concanavalin A and Escherichia coli lipopolysaccharide after stimulation with Mycobacteriurn bovis, strain BCG. (immunehealthscience.com)
  • a subset of human suppressor T-cells is also sensitive to ConA. (wikipedia.org)
  • ConA can also initiate cell division (mitogenesis), primarily acting on T-lymphocytes, by stimulating their energy metabolism within seconds of exposure. (wikipedia.org)
  • ConA strongly agglutinates erythrocytes irrespective of blood-group, and various cancerous cells. (wikipedia.org)
  • It was demonstrated that transformed cells and trypsin-treated normal cells do not agglutinate at 4 °C, thereby suggesting that there is a temperature-sensitive step involved in ConA-mediated agglutination. (wikipedia.org)
  • ConA-mediated agglutination of other cell types has been reported, including muscle cells , B-lymphocytes (through surface Immunoglobulins), fibroblasts, rat thymocytes, human fetal (but not adult) intestinal epithelial cells, and adipocytes. (wikipedia.org)
  • PHA and ConA bind and cross-link components of the T cell receptor, and their ability to activate T cells is dependent on expression of the T cell receptor. (wikipedia.org)
  • in the study by Lei and Chang, ConA was found to be sequestered more by hepatic tumor cells, in preference to surrounding normal hepatocytes. (wikipedia.org)
  • Internalization of ConA occurs preferentially to the mitochondria after binding to cell membrane glycoproteins, which triggers an autophagic cell death. (wikipedia.org)
  • GML treated T cells have decreased cellular proliferation when stimulated with phorbol 12-myristate 12-acetate (PMA), concanavalin A (ConA), and Toxic Shock Syndrome Toxin-1 (TSST-1) 13 . (nature.com)
  • This product contains Concanavalin A (ConA) conjugated to paramagnetic microspheres. (tebubio.com)
  • The ConA magnetic beads are therefore useful to immobilize cells or nuclei presenting these glycans in their extracellular matrices. (tebubio.com)
  • Flow cytometric analysis of CD152 expression on stimulated Human peripheral mononuclear cells. (bdbiosciences.com)
  • Peripheral blood mononuclear cells were stimulated with Concanavalin A for 3 days, then stained with either Purified NA/LE Mouse IgG2a, κ Isotype Control (Cat. (bdbiosciences.com)
  • 1. Incubate 100 µl concanavalin-A-stimulated 3-day peripheral blood mononuclear cells (1x10^6) with primary (unconjugated) antibody for 20-30 minutes at room temperature. (bdbiosciences.com)
  • Objective To determine the effect of vitamin D3 on interferon-β (IFN-β) response and immune regulation in MS mononuclear cells (MNCs). (neurology.org)
  • Cytokine production in the skin and in circulating re-stimulated peripheral blood mononuclear cells (PBMC) demonstrated a mixed pro-inflammatory Th2/Th17 profile, with transcription of IL-4, IL-13, IL-6 and IL-17. (biomedcentral.com)
  • However, human blood cells, regardless of the multiplicity of the graft, retained their functional reactivity and were able to actively secrete the majority of the analyzed cytokines (8 of 10) in response to stimulation with concanavalin A. Diagnostically significant changes in the concentration of IFN γ , TNF α and IL-4 were detected for the characteristics of a specific antiplague response. (iimmun.ru)
  • Native Chromatin에서 Protein-DNA complex를 분리하기 위해 Target-specific Primary Antibody와 pAG-MNase를 활용하는 새로운 기술. (seoulin.co.kr)
  • 2. Primary Antibody와 pAG-MNase Fusion Enzyme이 핵으로 유입되는 것을 촉진시키기 위해 Digitonin으로 Cell Membrane Permeabilization. (seoulin.co.kr)
  • 3. Target-specific Primary Antibody는 Antibody와 Fusion Enzyme의 pAG Domain의 Protein-protein 상호작용을 통해 pAG-MNase 을 Chromatin에 위치하게 함. (seoulin.co.kr)
  • Protein conjugated pneumococcal vaccine elicit antibody responses that are T-cell dependent. (medscape.com)
  • Concanavalin A interacts with diverse receptors containing mannose carbohydrates, notably rhodopsin, blood group markers, insulin-receptor the Immunoglobulins and the carcino-embryonary antigen (CEA). (wikipedia.org)
  • These abnormalities might be due to abnormal distribution of concanavalin-A receptors on the neutrophils or a cytoskeletal/microtubular abnormality. (medscape.com)
  • In activating cells, NF-κB signaling is activated through a series of signaling cascades, following the ligation of various cell surface receptors with paired ligands. (xiahepublishing.com)
  • The authors analyzed proliferative activity of T cells and B cells, cytokine production and release, activity of matrix metalloproteinases (MMP), and surface expression of activation markers, adhesion molecules, and chemokine receptors on both T and B cells. (dr-niederwieser.at)
  • A concanavalin-A-induced suppressor cell assay was carried out in 10 patients with chronic filarial disease, 13 asymptomatic microfilaria carriers and 7 healthy subjects. (karger.com)
  • Luminal antigens are transported across the intestinal epithelium to stimulate the Peyer's patch pre-B-cells in the follicles that proliferate and migrate to distant sites. (nih.gov)
  • To address these knowledge gaps, we treated human activated peripheral blood T cells (APBTs) with solubilized GML or ethanol vehicle control. (nature.com)
  • Co-incubation of rFg-CaBP4 with concanavalin A-stimulated monocytes increased the transcription levels of interleukin (IL)-2, IL-4, interferon gamma and transforming growth factor-β. (biomedcentral.com)
  • Results Vitamin D enhanced in vitro IFN responses, as measured by induction of p-Y-STAT1 and MxA in MNCs, T cells, and monocytes. (neurology.org)
  • Evidence suggests that antigen priming of B-lymphocytes is responsible for the number and location of Peyer's patches during postnatal life, but little is known about the histogenesis of Peyer's patches and their overlying membranous (M) cells. (nih.gov)
  • To examine whether T- and B-lymphocytes in Peyer's patches have an influence on M-cell generation, we studied the development of Peyer's patches and M-cells in severe combined immunodeficient (SCID) mice reconstituted with bone marrow cells from normal syngeneic mice. (nih.gov)
  • 20. Melchers, F.: Biochemical characterization of immunoglobulin-producing B-lymphocytes at different stages of their differentiation.In: 'Regulation of Growth and Differentiated Function in Eukaryotic Cells,' eds. (uni-mainz.de)
  • The development of skin sensitization is associated with, and requires, the activation and clonal expansion of allergen responsive T lymphocytes and it is these cells that orchestrate the cutaneous allergic reaction. (cdc.gov)
  • However, with regard to cellular immunity, a suppressive effect on concanavalin A- or crude parasite extract-induced splenocyte proliferation in these mice was observed upon addition of Em492 antigen, but trypan blue exclusion tests and transmission electron microscopy failed to reveal any cytotoxic effect in Em492 antigen-treated spleen cells. (unibe.ch)
  • In: 'Control of Proliferation in Animal Cells', Cold Spring Harbor Laboratory, pp. 393-410, 1974. (uni-mainz.de)
  • Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
  • Here we test this model by evaluating the impact of MLL3/4 loss on chromatin and transcription during early differentiation of mouse embryonic stem cells. (biomedcentral.com)
  • Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
  • In embryonic avian tendon, cell density regulates collagen production. (embl.de)
  • Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule. (academicinfluence.com)
  • Our experiments demonstrate that the donor bone marrow cells in the host scid mice repopulate to form single (primary) follicles and aggregates of lymphoid nodules, the Peyer's patches. (nih.gov)
  • Use of the lectins Anguilla anguilla (AAA) and Ulex europaeus I (UEA-I) as positive markers of mouse Peyer's patch M-cells revealed that M-cells develop in the dome epithelium overlying the single primary follicles and Peyer's patches of reconstituted scid mice. (nih.gov)
  • Anti-Em492 immunoglobulin G1 (IgG1) and IgG2 and, at lower levels, IgG3 were found in sera of mice suffering from experimentally induced secondary, but not primary, AE. (unibe.ch)
  • Role of CpG ODN in concanavalin A-induced hepatitis in mice. (fmu.ac.jp)
  • A similar pattern has been described in Sarcoptes scabiei infested mice and dogs with transcription of Th2 like cytokine profiles in lymph node cells and PBMC respectively [ 17 , 18 ]. (biomedcentral.com)
  • The effect of 20 g/100 g diet of lactalbumin (L), casein (C), soy (S) and wheat (W) protein on the immune responsiveness of C3H/HeN mice has been investigated by measuring the humoral immune response to the T cell-independent antigen, TNP-Ficoll. (immunehealthscience.com)
  • Abstract: Disclosed are methods of reducing lung inflammation in acute respiratory distress syndrome elicited by various factors such as COVID-19 infection by reduction of neutrophil extracellular trap formation through administration of mesenchymal stem cells and/or exosomes thereof. (therapeuticsolutionsint.com)
  • Abstract: Various cells, stem cells, and stem cell components, includ-ing associated methods of generating and using such cells are provided. (therapeuticsolutionsint.com)
  • The suppressive effects of MSC-derived exosomes and the MSCs in this injury model were evaluated by the level of plasma alanine aminotransferase (ALT), histopathological examinations, the messenger RNA (mRNA) expression of pro- and anti-inflammatory cytokines, and the population alteration of regulatory T cells (Treg) among non-parenchymal liver cells (NPCs). (biomedcentral.com)
  • For these marker cytokines, the greatest number of correlations have been revealed, both with each other and with changes in the level of other parameters: cytokines, immunoglobulins, T cells. (iimmun.ru)
  • Further research is needed to identify potential cell sources and biological functions for these cytokines and to fully unravel the basis of this different breed susceptibility to P. ovis . (biomedcentral.com)
  • In recent years, much has been learned of the characteristics of immune responses to skin sensitizing chemicals and of the roles played by dendritic cells, cytokines and chemokines. (cdc.gov)
  • In another embodiment stem cells are administered together with endothelial progenitor cells in order to regenerate neural endothelium. (therapeuticsolutionsint.com)
  • essential function in mediating neural progenitor cell function which the Shh signaling pathway is certainly involved with up-regulating miR17-92 cluster appearance. (epf2013.org)
  • This study aimed to evaluate the effect of mesenchymal stem cell (MSC)-derived exosomes on an immune-induced liver injury model. (biomedcentral.com)
  • Here we identify a vertebral skeletal stem cell (vSSC) that co-expresses ZIC1 and PAX1 together with additional cell surface markers. (bvsalud.org)
  • Together, our results indicate that vSSCs are distinct from other skeletal stem cells and mediate the unique physiology and pathology of vertebrae, including contributing to the high rate of vertebral metastasis. (bvsalud.org)
  • The identity of the calvarial stem cells (CSCs) that produce fusion-driving osteoblasts in craniosynostosis remains poorly understood. (bvsalud.org)
  • a previously identified cathepsin K (CTSK) lineage CSC1 (CTSK+ CSC) and a separate discoidin domain-containing receptor 2 (DDR2) lineage stem cell (DDR2+ CSC) that we identified in this study. (bvsalud.org)
  • DDR2+ CSCs display full stemness features, and our results establish the presence of two distinct stem cell lineages in the sutures, with both populations contributing to physiologic calvarial mineralization. (bvsalud.org)
  • The interaction between these two stem cell populations provides a new biologic interface for the modulation of calvarial mineralization and suture patency. (bvsalud.org)
  • Here, we identify a new cell type defined by co-expression of skeletal stem cell and adipocyte precursor markers, 9-13 (CD24+CD29+ skeletal stem cells (SSCs)), that serves as a key cellular point of bifurcation between the osteoblast and adipocyte differentiation pathways, giving rise to closely related osteoblast and adipocyte lineage-restricted precursors. (bvsalud.org)
  • The invention provides means of inhibiting neutrophil release of extracellular traps by mesenchymal stem cells and/or exosomes derived from said mesenchymal stem cells. (therapeuticsolutionsint.com)
  • Additionally, synergies are provided between mesenchymal stem cells and/or exosomes derived from mesenchymal stem cells and agents approaches which reduce neutrophil extracellular trap formation. (therapeuticsolutionsint.com)
  • To determine safety and efficacy of 100 million intravenously administered CTEcells™ allogeneic umbilical cord mesenchymal stem cells. (therapeuticsolutionsint.com)
  • In one embodiment the invention teaches the treatment of chronic traumatic encephalopathy (CTE) through protecting/regenerating the endothelial by administration of cells such as stem cells. (therapeuticsolutionsint.com)
  • In one embodiment stem cells are administered in order to protect the endothelium from apoptosis and to preserve the blood brain barrier. (therapeuticsolutionsint.com)
  • In other embodiments preservation of brain integrity in conditions of degeneration is accomplished by administration of stem cells and/or endothelial cells. (therapeuticsolutionsint.com)
  • To determine safety and efficacy of intravenously administered allogeneic JadiCell umbilical cord blood mesenchymal stem cells in patients with moderate-to-severe COPD. (therapeuticsolutionsint.com)
  • A generalized marrow dysfunction with an abnormal bone marrow stroma (in terms of its ability to support and maintain hematopoiesis) is thought to be present in addition to a stem cell defect. (medscape.com)
  • Endogenous stem cell therapy, aiming at replacing lost tissue and repairing damaged ones by endogenous progenitors, may offer hope. (edu.au)
  • Investigation into the reaction of endogenous progenitors in PTS may extend our knowledge about stem cell biology and help to develop a new treatment option for PTS. (edu.au)
  • Endogenous stem cells were found to differentiate into astrocytes. (edu.au)
  • In the chronic stage, the majority of stem cells are located in and around the lesion site. (edu.au)
  • These studies are important in understanding the endogenous stem cell response to PTS and lay the groundwork for future studies examining stem cell therapy for the condition. (edu.au)
  • Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
  • Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein. (academicinfluence.com)
  • Conventional dendritic cells regulate the outcome of colonic inflammation independently of T cells. (fmu.ac.jp)
  • Plasmacytoid dendritic cells (pDCs) perform dual proinflammatory and immunosuppressive roles. (keio.ac.jp)
  • Mechanisms of skin sensitization and allergic epidermal Langerhans cells (LC), although other cuta- contact dermatitis neous (dermal) dendritic cells (DC) may also contrib- ute. (cdc.gov)
  • Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. (gbiosciences.com)
  • Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. (gbiosciences.com)
  • It is a useful marker for endothelial cells from nonprimates such as mouse, rat, rabbit, and goat as well as a marker for non-peptidergic unmyelinated primary afferent neurons. (vectorlabs.com)
  • 11. Melchers, F.: Synthesis, transport and secretion of immunoglobulin in lymphoid cells. (uni-mainz.de)
  • Group 2 innate lymphoid cells (ILC2s) serve as frontline defenses against parasites. (keio.ac.jp)
  • This experimental system therefore allows the study of the histogenesis of Peyer's patches and M-cells. (nih.gov)
  • Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
  • In line with serving as the osteoblast/adipocyte bipotent cell, the "bone to fat" tissue remodeling occurring in models of postmenopausal osteoporosis or after high fat diet exposure occur in part by reprogramming these CD24+CD29+SSCs to change their output of lineage-restricted precursors. (bvsalud.org)
  • In one aspect, for example, an isolated cell that is capable of self-renewal and culture expansion and is obtained from a subepithelial layer of a mammalian umbili-cal cord tissue. (therapeuticsolutionsint.com)
  • In individuals with this condition, pancreatic acinar cells do not develop in utero and are replaced by fatty tissue. (medscape.com)
  • A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. (lookformedical.com)
  • Cell adhesion molecules in the regulation of animal form and tissue pattern. (academicinfluence.com)
  • Oral tolerance is a T-cell-mediated phenomenon described as the inhibition of immune responsiveness to a protein previously contacted by the oral route [ 1 , 2 ]. (hindawi.com)
  • Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
  • The Carboxyl Coupling Resin consists of 6% cross-linked agarose with covalent linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. (gbiosciences.com)
  • Although the development of cyclosporine A and tacrolimus, which suppress interleukin (IL)-2 expression and T cell-mediated immune responses, was a breakthrough, their adverse effects are still a major problem in the long-term follow-up of transplant recipients. (biomedcentral.com)
  • The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. (tebubio.com)
  • The NHS ester can be used to label the primary amines (-NH2) of proteins, oligonucleotides, and other amine-containing biomolecules. (tebubio.com)
  • Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
  • They range from small molecules such as primary and secondary metabolites and hormones to large macromolecules like proteins, nucleic acids, carbohydrates, lipids etc. (sarthaks.com)
  • Gene regulatory networks that drive cell fate are regulated spatiotemporally by cell-type-specific transcription factors (TFs). (biomedcentral.com)
  • Alternative sites for cell density regulation of the enzyme could rely on its sensitivity to sulfhydryl groups. (embl.de)
  • In: 'Regulation of the Immune System: Genes and the Cells in which They Function,' Vol. VI, eds. (uni-mainz.de)
  • Strong IL-17 up-regulation in the skin of BB was associated with an influx of eosinophils and other immune cells, potentially leading towards more severe symptoms. (biomedcentral.com)
  • This suggests that a cell that forms adipocytes at the expense of osteoblasts is central to the pathogenesis of skeletal disorders. (bvsalud.org)
  • The SBDS gene may not be required for neutrophil maturation but may act to maintain survival of granulocyte precursor cells. (medscape.com)
  • It shall be noted that these components impart a hydrophobic character to the mycobacterial cell surface that facilitates cell to cell interaction. (microbialcell.com)
  • Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface. (lookformedical.com)
  • In: Cell Surface Reviews 3:601-618, 1977. (uni-mainz.de)
  • However, how GML treatment affects the full T cell activation response from signaling events to effector functions is not well understood. (nature.com)
  • This autosomal-recessive genetic disorder typically leads to a severe combined immunodeficiency (SCID) with dysfunction of T, B, and natural killer (NK) cells (T-B-NK- SCID) that presents in the first few months of life ( table 1 ). (medilib.ir)
  • Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels. (embl.de)
  • In various proinflammatory cytokine-stimulated cells, ligand-receptor interactions initially activate TAK1. (xiahepublishing.com)
  • It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear. (lookformedical.com)
  • Proliferating cells were marked by either exogenous mitotic marker bromodeoxyuridine or endogenous mitotic marker Ki67.lmmunofluorescence techniques targeting mitotic markers were used to trace the proliferating cells. (edu.au)
  • The replicative activity of the cell dictates the level of sensitivity to these toxic metabolites. (medilib.ir)
  • There is a decrease in deoxyribonucleic acid (DNA) synthesis in replicating cells because of the depletion of deoxynucleotides. (medilib.ir)
  • PATHOGENESIS - ADA is a ubiquitous enzyme found in all cells, including red and white blood cells, and in the serum. (medilib.ir)
  • ADA concentration is highest in the thymus, which probably accounts for the dependence of T cell development and maturation upon this enzyme. (medilib.ir)
  • In addition, the mechanism behind how GML mediates suppressed T cell activation is completely unknown. (nature.com)
  • There are many advantages of studying bacteria in the planktonic cultures such as development of a homogenous population of bacterial cells having similar transcriptomic, proteomic and metabolomic profile etc. (microbialcell.com)
  • This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
  • The carbohydrate composition of a myeloma protein from different subcellular fractions of plasma cells. (uni-mainz.de)