Posterior lateral line zebrafish. Medical search. Frequent questions

Development of the posterior lateral line (PLL) originates from the migrating PLL primordium (PLLP). (ehu.eus)
Among these are Drosophila border cell migration, Zebrafish posterior lateral line primordium migration and Xenopus neural crest cell migration. (unc.edu)
Prim-6: primordium of the posterior lateral line (on the dorsal side), hatching gland (on the yolk ball). (ucsd.edu)
1 ] In the zebrafish lateral line primordium, a migrating cluster of approximately two hundred cells migrate, grow, divide and differentiate simultaneously and assemble into a series of connected epithelial rosette-like mechanosensory organs. (biomedcentral.com)

We expect to know more relatively soon on total numbers, but just to get a quick approximation: People have assumed in the past that there are ~100,000 neurons in the larval zebrafish at this age (we will know for sure for this fish soon). (psc.edu)

To further investigate candidate regulatory genes for lateral line development, we found using whole-mount in situ hybridization that calnexin , an endoplasmic reticular (ER) calcium-binding protein gene, is expressed in PLL neuromasts. (ehu.eus)
Transgenic expression of genes is a mainstay of cancer modeling in zebrafish. (bvsalud.org)
A model is presented in which zebrafish atoh1 genes operate in a complex network leading to hair cell development. (biologists.com)
Expression of esrra is colocalised with genes necessary for cartilage development in pharyngeal arches during zebrafish embryogenesis. (nature.com)
Heatmap of human deafness genes that are expressed in homeostatic lateral line scRNA-Seq data. (elifesciences.org)

Using a transgenic claudin b:gfp line, we observed a notably reduced PLLP size, but no significant migration defect in calnexin morphants. (ehu.eus)
The transgenic line is responsive to known Nrf2 pathway modulators but also to Edaravone, which direct activity on the Nrf2 pathway has never been documented in a live transgenic fish model. (bvsalud.org)
Therefore, this novel transgenic fish may represent a valuable tool to be exploited for the characterization of zebrafish models of human diseases, as well as for primary high-throughput drug screening. (bvsalud.org)
To explore the impact of Max dimerization protein 3 (MXD3), a transcription factor that regulates several cellular functions in disorders associated with metabolic diseases, we conditionally expressed Mxd3 proteins using Tet-on mxd3 transgenic zebrafish (MXs) with doxycycline (MXs + Dox) or without doxycycline (MXs - Dox) treatment. (bvsalud.org)
A detailed comparison of lhx5 -driven and vGLut2a -driven GFP in transgenic reporter lines revealed ancestral topological relationships between the thalamic eminence (EmT), the medial amygdala (MeA), the nLOT, and the integrative olfactory pallium. (frontiersin.org)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)
runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl , and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche . (biologists.com)
Wnt5a acts as an attractant for MSN axons in vitro , is expressed in a posterior high, anterior low gradient in the striatum, and Wnt5a knock-out mice phenocopy striatal anterior-posterior defects observed in Frizzled3 mutants. (jneurosci.org)
We focus on zebrafish mutants, in which photoreceptor degeneration is caused by defects in protein synthesis in ER, protein transport from ER to the apical photoreceptive membrane region, and phototransduction. (oist.jp)

In vertebrates a dorsal organizer initiates and elongates the midline towards anterior (prechordal plate) and towards posterior (the moving node). (mpg.de)
The blastopore and the organizer (dark green) riding on them remain the most posterior structure (anterior is to the left, dorsal is up/front). (mpg.de)
D) Expression of pqbp1 , wbp11 , chordin and sox2 in late gastrulae, dorsal- posterior views. (xenbase.org)

These results suggest that calnexin is essential for neuromast formation during lateral line development in the zebrafish. (ehu.eus)
One of eight distinct lateral lines in the 4-day larva consistingo of four neuromast that are positioned inferior or posterior to the eye A sensory system on the surface of the fish, consisting of small sensory patches (neuromasts) distributed in discrete lines over the body surface. (ontobee.org)

Neural crest cells in mice, Leghorn chicks, amphibians (Xenopus laevis), and fish (zebrafish): collective migration of neural crest cells occurs during embryo development of vertebrates. (wikipedia.org)

Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. (biologists.com)
The expression pattern of this gene family has been recently described during zebrafish ( Danio rerio ) embryogenesis, and primarily locates in the nervous system. (frontiersin.org)
In this report, we establish an in vivo role of Esrra in cartilage development during embryogenesis in zebrafish. (nature.com)
In this report, we used the zebrafish model to examine the role of esrra in cartilage development during vertebrate embryogenesis. (nature.com)

In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. (frontiersin.org)
Drosophila's lateral posterior neurons (LPNs) belong to a small group of circadian clock neurons that is so far not characterized in detail. (sdbonline.org)
Two of the neurons with similar morphology arborize in the superior medial and lateral protocerebrum and most likely promote sleep. (sdbonline.org)
These disorders include motor neuron diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA), which may involve motor neurons in the brain, spinal cord, and periphery, ultimately weakening the muscle. (medscape.com)

We also show that the reporter is faithfully activated during fin regeneration, and its degree of expression is slightly affected in a glucocerebrosidase (Gba1) morphant zebrafish model. (bvsalud.org)

ZO-1 expression is significantly reduced in many breast cancer lines. (thermofisher.com)
Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family. (frontiersin.org)
Moreover, zebrafish is very well-suited for systematic analysis of gene expression patterns at transcript level by whole-mount in situ hybridization (WISH), and at protein level by immunohistochemistry/immunofluorescence (IHC/IF). (frontiersin.org)
A complex expression pattern of ESRRs during animal development seems to be consistent with the potential roles for ESRRa during appropriate developmental programs of tissues and organs in mouse and zebrafish 9 . (nature.com)
Expression of zebrafish goosecoid and no tail gene-products in wild-type and mutant no tail embryos. (mpg.de)
Thanks to a new highly specific split-Gal4 line, this study describes LPNs' morphology in fine detail, their synaptic connections, daily bimodal expression of neuropeptides, and a putative role of this cluster in controlling daily activity and sleep patterns is proposed. (sdbonline.org)
Our results, along with those on Otx - and Hox -gene expression, demonstrate that the cephalopod condition is similar to that in mouse and fruit fly, with Otx being expressed in the anterior-most brain region (except for the vertical lobe) and a Pax2/5/8 expression domain separating the Otx -domain from a Hox -gene-expressing posterior brain region. (biomedcentral.com)

We analyzed this process in detail in zebrafish embryos using in vivo confocal microscopy and electron microscopy. (ehu.eus)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

The study introduces a long-needed testable molecular reference paradigm of the mature zebrafish extended amygdaloid complex studying neural underpinnings and evolution of emotion in this important model organism. (frontiersin.org)
We investigated the role of Col2a1a in neural crest migration and differentiation during early zebrafish eye development. (mdpi.com)

in vivo chemical screens in zebrafish. (tocris.com)

Molecular characteristics such as the distribution of substance P and the calcium-binding proteins parvalbumin (PV) and calretinin (CR) indicate, that the zebrafish extended centromedial (autonomic and reproductive) amygdala is predominantly located in the GABAergic and isl1 -negative territory. (frontiersin.org)

In zebrafish, a subset of hypaxial muscle precursors from the anterior somites undergo long-range migration, moving away from the trunk in three streams to form muscles in distal locations like the fin. (zfin.org)
Here, we implicate the Wnt binding receptor Frizzled3 in several uncharacterized aspects of MSN pathway formation [i.e., anterior-posterior guidance of MSN axons in the striatum and their subsequent growth into the globus pallidus (GP), an important (intermediate) target]. (jneurosci.org)
In Frizzled3 knock-out mice, MSN axons fail to extend along the anterior-posterior axis of the striatum, and many do not reach the GP. (jneurosci.org)
This suggests that Wnt5a controls anterior-posterior guidance of MSN axons through Frizzled3. (jneurosci.org)
Further, they are the first to identify a gene that provides anterior-posterior axon guidance in a large brain nucleus and link Frizzled3 to corridor cell development. (jneurosci.org)
Here, we determine the embryonic ontogeny of the two main striatal pathways (striatonigral and striatopallidal) and identify novel (non)cell-autonomous roles for the axon guidance receptor Frizzled3 in uncharacterized aspects of striatal pathway formation (i.e., anterior-posterior axon guidance in the striatum and axon entry into the globus pallidus). (jneurosci.org)
Since the blastopore is at the early gastrula stage the most posterior structure, the spot-like Spemann-type organizer (dark green), localized on the blastopore, gives rise to an anterior and to a posterior part of the midline The first ingressing mesodermal cells move towards anterior. (mpg.de)
lateral views except anterior (ant) view of neurula . (xenbase.org)
5 ] In isolated cells that do not interact with other cells and have unstable cell polarity, the chemoattractant SDF1 is unable to induce a distinct anterior-posterior polarity, and the cells chemotax poorly. (biomedcentral.com)
With an increase in cell density, the cells are able to interact with each other, and anterior-posterior polarity is established due to contact inhibition of locomotion at sites of interaction between cells. (biomedcentral.com)
One unique, possibly wakefulness-promoting, neuron with wider arborizations extends from the superior lateral protocerebrum toward the anterior optic tubercle. (sdbonline.org)

Wound healing is commonly studied in vitro using cell lines such as Madin-Darby Canine Kidney cells. (wikipedia.org)
To this end, the zebrafish ( Danio rerio ) is an established model to study early nervous system development, which combines the complexity of a vertebrate organism with the easy-to-use and high-throughput capabilities of in vitro models ( MacRae and Peterson, 2015 ). (frontiersin.org)

The May 10, 2017, issue of the prestigious journal Nature featured an article by researchers at Harvard University and PSC that charted out the first "nano-scale" reconstruction of a vertebrate brain-that of the zebrafish larva. (psc.edu)
What percentage of the zebrafish larva brain do we have mapped at this point? (psc.edu)

Cells that forms the more lateral mesoderm move towards the organizer and the incipient midline by the convergence-extension movement (red arrows). (mpg.de)

Here, we examine this question during the early stages of morphogenesis of the embryonic laterality organ, the first organ to form during vertebrate development, using the zebrafish as a model organism. (elifesciences.org)

The problem of multiple stripes cannot be circumvented by a strengthening of the lateral inhibition since this would lead to a decay of the stripe into individual patches. (mpg.de)

The lateral line in zebrafish: collective cell migration from head to tails is essential to the development of the sensory system of the fish. (wikipedia.org)
Two zebrafish homologs, atoh1a and atoh1b , are together necessary for hair cell development. (biologists.com)
Our data suggest that zebrafish Runx1 functions in both blood and vessel development at the hemangioblast level, and contributes to both primitive and definitive hematopoiesis. (biologists.com)
During normal development, the middle muscle precursor stream initially populates the fin bud, then the remainder of this stream contributes to the posterior hypaxial muscle. (zfin.org)
When we block fin bud development by impairing retinoic acid synthesis or Fgfr function, the entire stream contributes to the posterior hypaxial muscle indicating that muscle precursors are not committed to the fin during migration. (zfin.org)
Developmental neurobiology unit uses zebrafish as an animal model and will elucidate genetic program that regulate eye development. (oist.jp)

Through molecular characterization and evolutionary-developmental considerations, we delineated the complex amygdala ground plan of zebrafish, whose everted telencephalon has made comparisons to the evaginated forebrains of tetrapods challenging. (frontiersin.org)
Based on molecular and evolutionary-developmental characteristics, the study identifies the elaborate amygdala ground plan in zebrafish and stresses the evolution of a complex emotional system in early vertebrates. (frontiersin.org)

Progenitors of the zebrafish laterality organ originate from the superficial epithelial enveloping layer by an apical constriction process of cell delamination. (elifesciences.org)
6 ] In the commonly used epithelial cell line MDCK, cells in a monolayer migrated directionally to the anode in an electric field (EF), whereas isolated cells displayed random migration in an EF of the same strength [ Figure 1 ]. (biomedcentral.com)

These approaches can be performed in any genetic background such as existing fluorophore reporter lines or the casper line. (bvsalud.org)
Starting with a genetic screen in zebrafish, we discovered in alphaII-spectrin (alphaII-spn) a mutation that disrupts nodal sodium-channel clusters in myelinated axons of the PNS and CNS. (ed.ac.uk)

The posterior part of the midline (red) is left behind the moving organizer which contains a pool of stem cells that form in the course of time the notochord and the floorplate. (mpg.de)
Cluster 4 (differentiating divisions) and cluster 3 cells (amplifying support cells) now cluster with central and D/V support cells, respectively (dashed lines). (elifesciences.org)

The lateral line is a mechanosensory system in fish and amphibians to detect local water flow and pressure. (ehu.eus)

For example, we have applied light-activatable cMOs to interrogate transcription factor function during zebrafish notochord, pancreas, and vascular patterning. (cdc.gov)

Our findings demonstrate a conserved muscle precursor motility pathway, identify dynamic cell movements that generate posterior hypaxial and fin muscles, and demonstrate flexibility in muscle precursor fates. (zfin.org)

The sensors of the lateral line measure the flow over the body-surface of the fish. (wikipedia.org)
To this day, scientists have failed to consistently map pallial and subpallial (subcortical) territories even in relatively well-investigated teleost fish such as cichlids and cyprinids (carp-like fish) like goldfish or zebrafish. (frontiersin.org)
The lateral line system is stimulated by local water displacements and vibrations, and detects propulsion of the fish through the water, as well as facilitating shoaling, prey capture, and predator and obstacle avoidance. (ontobee.org)

These methods can readily be extended to other cell types allowing for rapid adult modeling of cancer in zebrafish. (bvsalud.org)

Here we provide evidence that in zebrafish,early DRG sensory neuron survival is independent of differentiated glia. (silverchair.com)

We provide a method to model cancer in adult zebrafish in which transgenes can be expressed via electroporation. (bvsalud.org)

Our results demonstrate that global actions of Mxd3 are central to the initiation of obesity in the gMX zebrafish through their effects on adipogenesis and that MXD3 could serve as a therapeutic target for obesity-associated liver diseases. (bvsalud.org)

The block in definitive hematopoiesis is similar to that observed in Runx1 knockout mice, implying that zebrafish Runx1 has a function equivalent to that in mammals. (biologists.com)

To further elucidate the role of RUNX1 in hematopoiesis, we cloned the zebrafish ortholog ( runx1 ) and analyzed its function using this model system. (biologists.com)

The mechanism for posterior midline formation may be compared with a high-flying airplane - a spot-like device - that leaves behind a stripe-like pattern - the vapour trail. (mpg.de)

In this radical paradigm, thirteen telencephalic territories constitute the zebrafish amygdaloid complex and each territory is distinguished by conserved molecular properties and structure-functional relationships with other amygdaloid structures. (frontiersin.org)
Ultimately the goal would be for a plasma membrane to be the target structure here, but for physical sectioning they're too thin, at something like 8 nm thickness (note that this sets the ~4 nm/pixel side target lateral resolution for "high resolution imaging" that most people use). (psc.edu)

Anatomy12

Organisms4

Chemicals and Drugs3

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes6

Health Care1

Primordium4

Larval zebrafish1

Genes5

Transgenic6

Mutants4

Dorsal3

Neuromast2

Xenopus1

Embryogenesis4

Neurons4

Regeneration1

Expression7

Embryos2

Neural2

Vivo1

Proteins1

Anterior11

Vitro2

Larva2

Mesoderm1

Vertebrate1

Inhibition1

Development6

Developmental2

Epithelial2

Genetic2

Cells2

Amphibians1

Transcription factor1

Pathway1

Fish3

Cell1

Early1

Adult1

Demonstrate1

Mice1

Model system1

Formation1

Structure2