Signalling pathwayPhosphorylationPolyclonal AntibodyAntibodyCTNNB1ProteinGSK3P120 cateninCadherinGlycogenInactivationAdherensPathwaysInhibitorPhosphorylatesPI3KWnt3aEndogenousCellsActivationTumorsAntibodiesProteins formInhibitExpressionSyntheticAlphaHumanLysate1996GenesRoleAminoMiceDomainCellPromoteGamma
Signalling pathway1
- GSK3β is also a pivotal regulator of the Wnt/β-catenin signalling pathway, which is closely related to the pathogenesis of AD ( 12 , 13 ). (spandidos-publications.com)
Phosphorylation9
- One consensus phosphorylation site is S/Tp-X-X-S/T, where S/Tp refers to a phospho-serine or phospho-threonine, X refers to any amino acid, and the underlined residues refer to the target site. (wikipedia.org)
- Casein kinase 1 alpha (CK1α) binds to and phosphorylates β‑catenin In plants the phosphorylation of protein Jade-1 is regulated by casein kinase 1. (wikipedia.org)
- Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of ADRBK1, leading to beta-arrestin phosphorylation and internalization. (drugbank.com)
- This review discusses the findings that support and those that argue against a direct effect of cadherin and catenin phosphorylation in the disassembly of the adherens junction. (hindawi.com)
- Lampugnani and co-workers showed that tyrosine phosphorylation of VE-cadherin and associated catenins is increased in loosely confluent endothelial monolayers, whereas the phosphorylation is diminished in tightly confluent monolayers [ 9 ]. (ijbs.com)
- Ozawa and Kemler showed that increased levels of tyrosine phosphorylation, induced by pervanadate promote the dissociation of alpha-catenin from the cadherin-catenin complex [ 10 ]. (ijbs.com)
- Phospho-kinase array was performed to detect Jun N-terminal kinase (JNK) phosphorylation. (biomedcentral.com)
- Mechanistically, CD58 activates the AKT/GSK-3β/β-catenin signaling pathway by increasing phosphorylation of AKT or GSK3β signaling, promoting expression of Wnt/β-catenin target proteins and TCF/LEF-mediated transcriptional activity. (biomedcentral.com)
- The protein β-catenin, an important element of the Wnt signaling pathway, and phosphorylation of β-catenin by Glycogen synthase kinase (GSK)-3β trigger its degradation in the cytoplasm and reverse the pro-cancer Wnt/β-catenin pathway [ 20 ]. (biomedcentral.com)
Polyclonal Antibody3
- The following product was used in this experiment: Phospho-beta Catenin (Thr41, Ser45) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-17916, RRID AB_10980964. (thermofisher.cn)
- The following product was used in this experiment: Phospho-ADD1/ADD2 (Ser726, Ser713) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-36614, RRID AB_2553614. (thermofisher.com)
- Samples were incubated with Phospho-ADD1/ADD2 (Ser726, Ser713) polyclonal antibody (Product # PA5-36614) at a dilution of 1:500. (thermofisher.com)
Antibody4
- Phospho-EGFR (Tyr992) Antibody detects endogenous levels of EGFR only when phosphorylated at Tyr1016, which site historically referenced as Tyr992. (affbiotech.cn)
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.cn)
- β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG ® M2 Antibody) #2368 and analyzed by western blotting using Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine/Pulmonary, University of Chicago). (cellsignal.cn)
- Western blot analysis of extracts from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes or λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser675) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (lower). (cellsignal.cn)
CTNNB12
- In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta-catenin (CTNNB1), delta-catenin (CTNND1), and plakoglobin (JUP). (drugbank.com)
- Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. (betalifesci.com)
Protein11
- Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. (thermofisher.cn)
- Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. (thermofisher.cn)
- e) Western blotting was used to determine the protein levels of AKT, p-AKT, GSK-3 β , p-GSK-3 β , β -catenin, and p- β -catenin. (bioz.com)
- Tamoxifen induces apoptosis through cancerous inhibitor of protein phosphatase 2A-dependent phospho-Akt inactivation in estrogen receptor-negative human breast cancer cells. (medscape.com)
- catenin beta interacting protein 1 [So. (gsea-msigdb.org)
- VE-cadherin is part of a protein complex comprising p120-catenin and beta-catenin as intracellular partners. (ijbs.com)
- It associates to the armadillo-family proteins beta- and gamma-catenin that bind the actin-binding protein alpha-catenin [ 1 , 2 ]. (ijbs.com)
- Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit. (embl.de)
- Members of this family include the signal-transducing G protein beta subunit, as well as other proteins that regulate signal transduction, transcription, pre-mRNA splicing, cytoskeletal organization, and vesicular fusion. (embl.de)
- The crystal structure of one WD-repeat protein (G beta) has now been solved (Wall et al. (embl.de)
- However, the only studied example of a WD-repeat protein, G beta, synthesized in vitro in a rabbit reticulocyte lysate, is unable to fold into a native structure without its partner protein G gamma. (embl.de)
GSK38
- In this study, we provide evidence that rapamycin inhibits GSK3β activation and elevates β‑catenin expression by improving the Wnt3a expression levels, which facilitates the amelioration of AD pathology. (spandidos-publications.com)
- In hair matrix keratinocytes, P-cadherin silencing reduced plasma membrane β-catenin, whereas glycogen synthase kinase 3 beta (GSK3β) and phospho-β-catenin expression were significantly upregulated. (uni-luebeck.de)
- The present study was undertaken to further determine if atypical antipsychotics, which have therapeutic effects in both mood disorders and schizophrenia, can regulate phospho-Ser-GSK3 and inhibit its activity. (researchgate.net)
- The results showed that acute treatment of mice with risperidone rapidly increased the level of brain phospho-Ser-GSK3 in the cortex, hippocampus, striatum, and cerebellum in a dose-dependent manner. (researchgate.net)
- Regulation of phospho-Ser-GSK3 was a shared effect among several atypical antipsychotics, including olanzapine, clozapine, quetiapine, and ziprasidone. (researchgate.net)
- In addition, combination treatment of mice with risperidone and a monoamine reuptake inhibitor antidepressant imipramine or fluoxetine elicited larger increases in brain phospho-Ser-GSK3 than each agent alone. (researchgate.net)
- disorders and schizophrenia, can regulate phospho-Ser-GSK3 and inhibit its activity. (researchgate.net)
- phospho-Ser-GSK3 than each agent alone. (researchgate.net)
P120 catenin2
- Similar to other classical cadherins, the cytoplasmic tail of VE-cadherin contains the binding regions for p120 catenin at the juxtamembrane domain (JMD) and for β -catenin or γ -catenin at the C-terminal catenin binding domain (CBD). (hindawi.com)
- Binding of p120 catenin stabilizes junctional cadherins by preventing cadherin endocytosis (reviewed in [ 3 , 10 ]), while β -catenin associates with α -catenin, providing the link to the actin cytoskeleton [ 11 , 12 ]. (hindawi.com)
Cadherin9
- Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. (thermofisher.cn)
- its basic structure comprises transmembrane cadherins and cytosolic catenins linking the cadherin to the cytoskeleton [ 3 ]. (hindawi.com)
- van Buul JD, van Alphen FP, Hordijk PL. The presence of alpha-catenin in the VE-cadherin complex is required for efficient transendothelial migration of leukocytes. (ijbs.com)
- Beta-catenin connects VE-cadherin to alpha-catenin. (ijbs.com)
- This VE-cadherin-catenin complex is believed to dynamically control endothelial cell-cell junctions and to regulate the passage of leukocytes, although not much is known about the role of alpha- and beta-catenin during the process of transendothelial migration (TEM). (ijbs.com)
- In conclusion, our results show for the first time that the association of alpha-catenin with the cadherin-catenin complex is required for efficient leukocyte TEM. (ijbs.com)
- Together, these proteins form the so-called cadherin-catenin complex. (ijbs.com)
- The catenins are thought to play an important role in the regulation of VE-cadherin function. (ijbs.com)
- Moreover, their results indicated that the binding of alpha-catenin to the cadherin-catenin complex is a prerequisite for proper functioning cadherin-catenin complex, since an E-cadherin-alpha-catenin chimera was insensitive to pervanadate treatment leaving E-cadherin trans-interactions intact, whereas cells expressing the normal cadherin-catenin complex, including beta-catenin, showed loss of cell-cell contact after pervanadate treatment [ 10 ]. (ijbs.com)
Glycogen1
- 20 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.2. (betalifesci.com)
Inactivation1
- We show that compound genetic inactivation of Sfrp1 and Sfrp2 prevents Wnt/β-catenin signalling activation in this structure, which fails to be specified and acquires neural retina characteristics. (biologists.com)
Adherens1
- The adherens junction, a major mechanism of intercellular adhesion, is comprised of transmembrane cadherins forming homotypic interactions between adjacent cells and associated cytoplasmic catenins linking the cadherins to the cytoskeleton. (hindawi.com)
Pathways3
- The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. (thermofisher.cn)
- Furthermore, analysis of hepatocellular carcinomas revealed that rare gp130 alterations are always accompanied by beta-catenin-activating mutations, suggesting a cooperative effect of these signalling pathways in the malignant conversion of hepatocytes. (nih.gov)
- The Wnt/β-catenin and PI3K/Akt pathways are among the most frequently mutated in cancer [ 19 ]. (biomedcentral.com)
Inhibitor2
- Reagents The antibodies aganist c-jun phospho-c-jun JNK phospho-JNK 3 To assess the way the miR-92b inhibitor added towards the apoptosis in glioma cells we looked into the gene focuses on of miR-92b by using the prediction device TargetScanHuman Launch 6.2. (cancerrealitycheck.com)
- 3 is undoubtedly a secreted antagonist from the Wnt/beta-catenin signaling pathway [25 26 Because this pathway can be always triggered in gliomas Rosiglitazone maleate [27-29] we hypothesized how the miR-92b inhibitor could play a pro-apoptotic part by inhibiting the Wnt/beta-catenin signaling pathway. (cancerrealitycheck.com)
Phosphorylates1
- In contrast, in several important targets, NF-AT and beta-catenin, CKI does not require n − 3 priming but, instead, phosphorylates the first serine in the sequence S-L-S, which is followed by a cluster of acidic residues, albeit less efficiently than the optimal sites. (wikipedia.org)
PI3K1
- Conclusion:The activation of PI3K/Akt-LEF1/β-catenin pathway could be suppressed synergistically by two agents and that PI3K/Akt-LEF1/β-catenin pathway is participated in the synergy of two agents. (1library.net)
Wnt3a1
- Moreover, activation of Wnt/β-catenin signaling via Wnt3a/R-Spo1 treatment protects these cells from regorafenib induced apoptosis. (frontiersin.org)
Endogenous1
- Detection of endogenous β-Catenin.Whole cell lysates of serum starved tumor cells (20'000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. (enzolifesciences.com)
Cells8
- Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. (thermofisher.cn)
- Western blot analysis of lysate of pervanadate-treated SKOV-3 cells (phospho- and dephospho-β-catenin) using various antibodies. (enzolifesciences.com)
- Effects of HA-ADT on the apoptotic level and AKT/GSK-3 β / β -catenin pathway in human HCC cells. (bioz.com)
- Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R. (thermofisher.com)
- In this study, we show that regorafenib treatment activates Wnt/β-catenin signaling only in hepatoblast-like HCC cell lines and induces enrichment of markers associated with hepatic stem/progenitor cells. (frontiersin.org)
- On the other hand, regorafenib resistant cells established by long-term regorafenib treatment demonstrate diminished Wnt/β-catenin signaling activity while TGF-β signaling activity of these cells is significantly enhanced. (frontiersin.org)
- COS-7 cells were transfected with cDNA encoding DYKDDDDK-tagged wild type (WT) β-catenin or Ser-to-Ala β-catenin mutants as indicated. (cellsignal.cn)
- Western blotting, immunofluorescence (IF), TOP/FOP Flash reporter assay, and subcellular fractionation assay were conducted to investigate the molecular regulation between CD58/sCD58 and AKT/GSK-3β/β-catenin axis in HCC cells. (biomedcentral.com)
Activation2
- In conclusion, our data suggest that HCC tumors with aberrant activation in the Wnt/β-catenin pathway, might have higher intrinsic regorafenib resistance and the inhibition of this pathway along with regorafenib administration might increase regorafenib-induced cell death in combinational therapies. (frontiersin.org)
- Transducin (β)-like 1 X-linked receptor 1 promotes proliferation and tumorigenicity in human breast cancer via activation of beta-catenin signaling. (medscape.com)
Tumors1
- La tinció immunohistoquímica es fa servir en el diagnòstic de cèl·lules anormals com les que es troben en els tumors cancerosos, entre altres afeccions. (wikipedia.org)
Antibodies1
- Western blot analysis of recombinant β-catenin from E. coli (mainly depospho-β-catenin) using various antibodies. (enzolifesciences.com)
Proteins form1
- If WD proteins form structures similar to G beta, their hydrodynamic properties should be those of compact, globular proteins, and they should be resistant to cleavage by trypsin. (embl.de)
Inhibit1
- The non-WD-repeat amino terminal alpha helix of G beta does not inhibit folding because G beta does not fold even when this region is removed. (embl.de)
Expression1
- 7. Tissue microarray-based analysis shows phospho-beta-catenin expression in malignant melanoma is associated with poor outcome. (nih.gov)
Synthetic1
- Synthetic peptide corresponding to human β-catenin aa 27-37 conjugated to KLH. (enzolifesciences.com)
Alpha2
- In order to study the importance of the interaction between alpha- and beta-catenin in TEM, we used a cell-permeable version of the peptide encoding the binding site of alpha-catenin for beta-catenin (S27D). (ijbs.com)
- The data show that S27D interferes with the interaction between alpha- and beta-catenin and induces a reversible decrease in electrical resistance of the endothelial monolayer. (ijbs.com)
Human1
- Pin1 is overexpressed in many human breast cancers and has been shown to modify numerous proteins, including p53, NF-kB, c-Jun, cyclin D1, and beta-catenin. (bio-techne.com)
Lysate2
- Western blot analysis of Phospho-ADD1/ADD2 (Ser726, Ser713) in Lane 1: HeLa cell lysate treated with PMA, Lane 2: SP2/0 cell lysate treated with PMA, Lane 3: PC12 cell lysate treated with PMA. (thermofisher.com)
- We show that unlike G beta, several proteins with WD-repeats are able to fold into globular proteins in a rabbit reticulocyte lysate. (embl.de)
19961
- Sondek et al, 1996) and reveals that the seven repeating units form a circular, propeller-like structure with seven blades each made up of four beta strands. (embl.de)
Genes3
- In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. (thermofisher.cn)
- To elucidate the system we recognized the proteins degree of beta-catenin as well as the downstream genes from the Wnt/beta-catenin signaling pathway such as for example Bcl2 c-myc c-Jun and p-c-Jun. (cancerrealitycheck.com)
- To verify whether miR-92b could modulate the Wnt/beta-catenin signaling pathway we assessed the manifestation from the downstream genes Bcl2 c-myc c-Jun and p-c-Jun by European blotting. (cancerrealitycheck.com)
Role1
- A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. (thermofisher.cn)
Amino1
- WD-40 repeats (also known as WD or beta-transducin repeats) are short ~40 amino acid motifs, often terminating in a Trp-Asp (W-D) dipeptide. (embl.de)
Mice1
- RESULTS: Levels of TGF-β, TGF-βR2, and phospho-Smad2/3 were increased in HCCs from Tak1ΔHep mice, which developed liver fibrosis and inflammation by 1 month and HCC by 9 months. (nih.gov)
Domain1
- Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain. (embl.de)
Cell1
- In addition, S27D co-localized with beta-catenin at cell-cell junctions. (ijbs.com)
Promote1
- PGE2 cooperates with Wnt4a to promote nephron maturation by regulating β-catenin stability of RPC aggregates. (elifesciences.org)
Gamma1
- Our working definition of folding was that the proteins from globular, trypsin-resistant structures because, except for G beta gamma, their functions are not known or cannot be assayed in reticulocyte lysates. (embl.de)