Phase novartis cells cultured. Medical search. Frequent questions

OAKLAND, Calif. , June 30, 2021 /PRNewswire/ -- Aditum Bio, the biotech investment firm co-founded in 2019 by former Novartis CEO Joe Jimenez and former President of the Novartis Institutes for BioMedical Research (NIBR) Dr. Mark Fishman , today announced the formation of Ancora Bio, a portfolio company developing a new therapy for treatment-resistant depression. (prnewswire.com)
9 Novartis Institutes for BioMedical Research, Basel, Switzerland. (aacrjournals.org)
10 Novartis Institutes for BioMedical Research, Cambridge, Massachusetts. (aacrjournals.org)
The Novartis Institutes for BioMedical Research (NIBR) collaborates across scientific and organisational boundaries. (move-upstream.org.uk)

They are usually considered to be safer than vaccines from bacterial cultures. (wikipedia.org)
Novartis just released the results of their phase 1 clinical trial of the cell-cultured H7N9 vaccines and found that only 6% of the 400 healthy subjects achieved a protective response when given two doses of the un-adjuvanted vaccine . (epivax.com)
JE-VC is manufactured by Intercell Biomedical (Livingston, United Kingdom) and distributed in the United States private market by Novartis Vaccines (Cambridge, Massachusetts). (cdc.gov)
Unlike conventional viral vaccines that inject weakened, non-replicating versions of a virus to present antigens and induce an immune response, the mRNA approach introduces engineered mRNA - single-stranded mRNA molecules that provide biological instructions for cells to produce proteins. (pharmaceutical-technology.com)
BioNTech aims to produce additional therapeutic and vaccine drug candidates at the plant, such as other mRNA vaccines, antibody, and cell and gene therapy product candidates to underpin the development of its diversified cancer and infectious disease product pipeline. (pharma-industry-review.com)
This week's headlines include, 217 vaccines in development, Roche enters into immunotherapy deal with Inovio, European Commision approves first biosimilar, Biogen and Isis pair up on neurological drugs, Novartis and Regenerex enter stem cell deal, FDA speeds approvals, and America Stem Cell gets FDA approval for nationwide trials. (cellculturedish.com)
These vaccines are designed to train immune cells to detect, target, and eliminate cancer cells. (personalizemymedicine.com)
The Imperial researchers see RNA vaccines offering significantly shorter lag phases than products that rely on viral vectors and mammalian cell culture. (outsourcing-pharma.com)

In addition, the novel cancer stem cell paradigm raises the prospect of cell-targeted therapies instead of treatment directed against the whole tumour. (nature.com)
Subsequent improved understanding of the molecular alterations present in the cancer cell has enabled the development of targeted therapies for some forms of cancer. (nature.com)
The U.S. Food and Drug Administration (FDA) approved two CAR T-cell therapies in 2017, Kymriah developed by Novartis for the treatment of children with acute lymphoblastic leukemia and Yescarta developed by Kite for adults with advanced lymphomas. (nature.com)
Dr. Miller has over 20+ years of business development, scientific research, product development and clinical operations expertise, with a focus on transitioning novel gene and cell therapies through pre-clinical and Phase 3 human clinical trials. (xeromedia.com)
The plant is fully equipped with the latest technology for the production of recombinant proteins as well as cell and gene therapies, and holds cell culture labs and viral vector production capabilities, with further potential for long-term growth and expansion. (pharma-industry-review.com)
[i] The License Agreement grants Novartis the exclusive worldwide rights to develop, manufacture and commercialize these cell therapies, and Novartis may apply its T-Charge platform to their manufacture. (outsourcedpharma.com)
A good example of these therapies are chimeric antigen receptor T cell (CAR-T) therapies. (pall.com)
Both Novartis' Kymriah ♦ and Gilead's Yescarta ♦ are examples of CAR-T therapies, that have demonstrated exceptional cancer remission rates where other forms of treatment have failed. (pall.com)
An immunologist by training, Dr. Rafei is an expert in cellular and molecular immunology with major focus on cell-based therapies and therapeutics. (newswire.ca)

Next-gen immunotherapy company BioNTech announced this month the completion of a share purchase agreement with Swiss pharma giant Novartis AG, to acquire their GMP certified manufacturing site located in Marburg, Germany. (pharma-industry-review.com)
Financial support: the study was founded by Novartis Pharma A.G. (bace-signal.com)

The Wellcome Trust's Pathfinder Award of $244,000 will support a collaboration between UGA and the Novartis Institute for Tropical Diseases, a public-private partnership between the pharmaceutical company Novartis and the Singapore Economic Development Board. (uga.edu)
Mr. Amoroso continued, "We also advanced multiple in vivo gene editing programs, independently and with partners, highlighted by our research collaboration with Novartis in June and recent preclinical data shared at ESGCT. (biospace.com)
Now, Children's Hospital of Philadelphia (CHOP) scientists, in collaboration with Novartis researchers, decided to see if they could adapt LMI070's RNA splicing capabilities to regulate the expression of other genes. (scienceboard.net)
In August 2015, Amgen entered into a global collaboration with Novartis to jointly develop and commercialize pioneering treatments in the field of migraine and Alzheimer's disease (AD). (amgen.com)
The collaboration focuses on investigational Amgen drugs in the migraine field, including erenumab (currently in Phase 3 studies for episodic migraine and a Phase 2 study for chronic migraine) and AMG 301 (currently in a Phase 1 study for migraine). (amgen.com)
All reasonable requests for collaboration involving materials used in the research will be fulfilled provided that a written agreement is executed in advance between Novartis and the requester (and his or her affiliated institution). (bace-signal.com)

An abstract on the cell dose and functional attributes of azer-cel that may be associated with positive safety and efficacy results for CAR T therapy in R/R B-cell lymphoma was accepted for poster presentation at the 64th ASH Annual Meeting taking place December 10-13, 2022. (biospace.com)
Basel, September 10, 2022 - Novartis announced the results from two pivotal, Phase III studies (SUNSHINE and SUNRISE), in which Cosentyx ® (secukinumab) demonstrated rapid and sustained relief from the common clinical signs and symptoms of moderate-to-severe hidradenitis suppurativa (HS) with a favorable safety profile 5,6 . (novartis.com)
Legend Biotech is initiating clinical development of LB2102 for the treatment of extensive stage small cell lung cancer and large cell neuroendocrine carcinoma after the U.S. Food and Drug Administration (FDA) cleared its investigational new drug application in 2022. (outsourcedpharma.com)

Hormone treatment slows the growth and proliferation of breast cancer cells, but it does not kill them. (cancernetwork.com)
The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
Cell proliferation was determined by trypan blue dye exclusion test. (biomedcentral.com)
In this work, for the first time we have demonstrated that ATRA affected on cell proliferation of GIST-T1 and GIST-882 cell line through inhibition of cell growth in a dose dependent manner and induced apoptosis. (biomedcentral.com)
Thereafter, the downstream signaling pathways are activated promoting cell proliferation and/or survival. (biomedcentral.com)
Upon recognition of a foreign antigen, T cells become activated, launching a massive proliferation and releasing chemical messengers such as cytokines which act on the T cells to further stimulate them (Figure 1). (personalizemymedicine.com)

Some HDAC inhibitors lead to a dramatic correction in the NPC phenotype in cells with either one or two copies of the NPC1I1061T mutation, and for several of the inhibitors, correction is associated with increased expression of NPC1 protein. (addiandcassi.com)
The truncated form is degraded within the cell almost immediately, but the small percentage of SMN2 mRNAs which are successfully translated into full-length SMN protein can lessen the severity of SMA (caused by the absence of a functional SMN1 gene). (scienceboard.net)
Although Novartis has since abandoned development of LMI070 in favor of newer therapeutics for the treatment of SMA, clinical trial data from the SMA study indicated that the drug also decreased expression of huntingtin protein, an important therapeutic target for Huntington's disease. (scienceboard.net)
Serum amyloid A (SAA) is an acute-phase protein, upregulated in sera of RA patients. (hindawi.com)
The first report in 2007, on SAA-stimulated human coronary artery endothelial cells (HCAEC), exhibited a substantial and significantly higher induction of released IL-6 protein and mRNA levels as compared to HUVEC [ 17 ], as well as increased responsiveness to IL-1 β [ 18 ]. (hindawi.com)
Background Anti-programmed cell death protein 1 (PD-1) antibodies are now routinely administered for metastatic melanoma and for increasing numbers of other cancers, but still only a fraction of patients respond. (bmj.com)
High dose of ATRA induced morphologic change in GIST-T1 cells, rounded-up cells, and activated the caspase-3 protein. (biomedcentral.com)
In further examination, we found that the ATRA-induced apoptosis in GIST-T1 cells was accompanied by the down-regulated expression of survivin and up-regulated expression of Bax protein. (biomedcentral.com)
Moreover, ATRA suppressed the activity of KIT protein in GIST-T1 cells and its downstream signal, AKT activity, but not MAPK activity. (biomedcentral.com)
Antigen-specific CD40L-activated B cells may efficiently endocytose and present antigens, such as protein, RNA, and cDNA, to prime primary T cells and boost robust memory T-cell responses [ 9 ]. (biomedcentral.com)
This extracellular domain is connected to the transmembrane protein that anchors the CAR to the cell via a spacer region. (personalizemymedicine.com)

The infused T cells traffic to tumors and can mediate their destruction. (biopharmconsortium.com)
The agreement is based on one of the improvements to ACT discussed in the December 2011 Nature cancer immunotherapy review , in which autologous T cells isolated from patient blood (not from tumors) are engineered with retroviral vectors carrying chimeric antigen receptors (CARs). (biopharmconsortium.com)
[iv] LB2102 would be the first application of T-Charge by Novartis to a cell therapy candidate targeting solid tumors. (outsourcedpharma.com)
Furthermore, MDNA19 treatment of B16F10 tumors favored activation of CD8 T cells over Tregs in the tumor microenvironment driving a strong therapeutic effect. (newswire.ca)
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms occurring throughout the entire region of the gastrointestinal tract and are considered to originate from intestitial cells of Cajal, the pacemaker cells of the gut [ 1 ]. (biomedcentral.com)
Cytokine-based immunotherapies harness the power of cytokines, which are soluble proteins that stimulate and direct immune cells, to potentiate an immune response against tumors. (personalizemymedicine.com)
Each T cell harbors a unique TCR that can recognize and differentiate between specific marker molecules known as antigens that are displayed on the surface of healthy host cells, pathogens, and tumors. (personalizemymedicine.com)

We hope to offer Cosentyx as a potential new treatment option as soon as possible to support this underserved patient community, as part of our ambition to expand Cosentyx to 10 indications," said Todd Fox, Global Head of Medical Affairs Immunology, Novartis. (novartis.com)

Interestingly, Gleevec , another cancer drug developed by Novartis also works in the NPC mouse model (modestly) and was reported by Paul Greengard to have an effect in the Alzheimer's model. (addiandcassi.com)

Autologous mature dendritic cells derived from peripheral blood monocytes were pulsed with the HLA-A2-binding MUC1 peptides (M1.1 and M1.2). (aacrjournals.org)
Methods Peripheral blood T cell stimulation by NY-ESO-1 and Melan-A overlapping peptide libraries was assessed before and during ICB in two independent cohorts of a total of 111 patients with stage IV melanoma. (bmj.com)
As a terminal cell type, B cells in peripheral blood mononuclear cells (PBMCs) can usually be cultured for 2-3 weeks only, which limits the application of B cells as APCs. (biomedcentral.com)

In the adult mammalian subventricular zone (SVZ), GFAP-positive neural stem cells (NSCs) generate neuroblasts that migrate tangentially along the rostral migratory stream (RMS) toward the olfactory bulb (OB). (jneurosci.org)
They manufacture biopharmaceutical products using mammalian cell culture at their Tuas facility. (simibest.sg)

For the migraine program, Amgen retains commercialization rights in the U.S., Canada and Japan , and Novartis has commercialization rights in Europe and rest of world. (amgen.com)

There is also the need for an appropriate magnitude of antigen-specific T cells to clear the tumor. (personalizemymedicine.com)

In partnership with TrialSpark, Aditum Bio uses data, software and technology to help bring innovative medicines through the clinical trial phase more quickly and with lower costs than traditional pharmaceutical companies. (prnewswire.com)
We are excited that a major pharmaceutical company with deep roots in oncology and cell therapy has chosen to further this product candidate in the clinic. (outsourcedpharma.com)
More than 25 years pharmaceutical development experience, mostly at Novartis, in both the early and late development arena. (newswire.ca)
The investigators checked more than 1000 pharmaceutical compounds in cell culture and found that the beta-2 adrenoreceptor can modulate the expression of synuclein. (medscape.com)

The preclinical evidence shows that an autologous CAR-T could be a differentiated treatment option for patients with small cell lung cancer," said Guowei Fang, Chief Scientific Officer and Head of Business Development of Legend Biotech. (outsourcedpharma.com)
MDNA19 is effective in both directions (diminishing binding to CD25 while increasing affinity to CD122) and the preclinical data demonstrate MDNA19's promise to restore both NK cell and memory CD8 T cell compartments in both small rodent and non-human primate models. (newswire.ca)

March 15, 2019 - Biohaven announced that the company has initiated patient enrollment in a Phase 3 clinical trial of its third generation pro-drug troriluzole for the treatment of SCA. (ataxia.org)

LBH589 (panobinostat) is an orally available HDAC inhibitor that crosses the blood-brain barrier and is currently in phase III clinical trials for several types of cancer. (addiandcassi.com)
Phases 1 through 3 involve clinical trials with human participants. (ataxia.org)
Visit our Clinical Trials page to learn more about each phase. (ataxia.org)
Aditum Bio focuses on basic mechanisms of disease, in-licenses promising drug candidates directed at such pathophysiology's, and spins-out individual companies dedicated to bringing each candidate through Phase II clinical trials. (prnewswire.com)
Novartis began phase I/II clinical trials for LMI070 in 2015 for the treatment of severe SMA in children with two copies of the SMN2 gene. (scienceboard.net)
Novartis began clinical trials for LMI070 in the treatment of Huntington's disease this year. (scienceboard.net)
To conduct such trials, a company would need to build a specialized cell processing and treatment facility, with a highly trained and competent staff. (biopharmconsortium.com)
Novartis also plans to share the long-term results from the trials in 2023. (novartis.com)
1000 clinical trials underway, and 92 drugs in Phase 3 2 . (pall.com)
The readout of our Phase 3 study is going to happen in December," she added of the pivotal study in the clinical trials process. (pedfire.com)
Novartis, also in the race, said in April that no signs of liver damage had been seen in trials testing its anti-inflammatory drug candidate remibrutinib so far. (pedfire.com)
Precis: Pulsed high doses versus sustained low doses of the p53-MDM2 inhibitor HDM201 elicit a pro-apoptotic response from wild-type p53 cancer cells, offering guidance to current clinical trials with this and other drugs that exploit the activity of p53. (bace-signal.com)
Now all phase 2 trials and the initial part of a phase 3 trial have been published. (medscape.com)

Novartis Vaccine and Diagnostics, among other companies, developed a synthetic approach that very rapidly generates vaccine viruses from sequence data in order to be able to administer vaccinations early in the pandemic outbreak. (wikipedia.org)
Phase I data of UB-311, a synthetic peptide vaccine targeting amyloid beta, showed that the drug was able to generate antibodies to specific amyloid beta oligomers and fibrils with no decrease in antibody levels in patients of advanced age. (wikipedia.org)
Inactivated Vero cell culture-derived Japanese encephalitis vaccine (JE-VC [manufactured as IXIARO]) is the only JE vaccine licensed and available in the United States. (cdc.gov)
The primary policy question was "Should inactivated Vero cell culture-derived Japanese encephalitis vaccine (JE-VC) be recommended for use in children 2 months through 16 years of age at increased risk of travel-related exposure to Japanese encephalitis virus? (cdc.gov)
The cost of ACT is, however, much lower than a full course of other immunotherapies, such as the dendritic cell vaccine Provenge (which is not indicated for melanoma) or the immunotheraputic MAb drug ipilimumab, both of which cost approximately $120,000. (biopharmconsortium.com)

Panobinostat inhibits one or more of the histone deacetylase (HDAC) enzymes, a mechanism leading to apoptosis of malignant cells via multiple pathways. (addiandcassi.com)
The generation of cellular models of drug resistance has been pivotal in unravelling the main effectors of resistance to traditional chemotherapy at the molecular level (i.e. intracellular drug inactivation, detoxifying systems, defects in DNA repair, apoptosis evasion, membrane transporters and cell adhesion). (nature.com)
The new studies show that BKM120 and BGT226 both induced high levels of apoptosis in ER+ breast cancer cell lines, notably those with mutations in PIK3CA but also some with mutations in PTEN, which reverses the catalytic actions of PI3K. (cancernetwork.com)
It required higher concentrations of BKM120 and BGT226 to reach the same level of apoptosis in these estrogen-deprived cells, but adding fulvestrant (which down-regulates the estrogen receptor) strongly potentiated cell death. (cancernetwork.com)

Chimeric antigen receptor (CAR) T-cell therapy is a rapidly growing therapy for the treatment of cancer 1 . (nature.com)
DLL3-Directed Chimeric Antigen Receptor T-cells in Subjects With Extensive Stage Small Cell Lung Cancer. (outsourcedpharma.com)
Here, S1P receptor knock-out mice, primary astrocyte cultures, and receptor-selective chemical tools were used to examine the effects of S1P on glutamate uptake. (eneuro.org)
One of the most promising ACT approaches that has attracted the greatest attention, is chimeric antigen receptor (CAR) T cell therapy. (personalizemymedicine.com)
T cells carry out their functions via a set of specialized proteins that are present on their cell surfaces, and which together form the T cell receptor (TCR). (personalizemymedicine.com)
This receptor allows T cells to receive signals from their external environment and to then relay these signals to the cell to initiate appropriate cellular changes. (personalizemymedicine.com)

She previously served as Executive Medical Director of Oncology Clinical Development at Novartis. (ichnossciences.com)
Following the approval of the IND, it will be time to move to the clinical development phase. (digitalhealthcentral.com)

Novartis' oral therapy CNP520 (currently in a Phase 1/2a study for AD) will be the lead molecule and further compounds from both companies' pre-clinical BACE inhibitor programs may be considered as follow-on molecules. (amgen.com)
The modest effects of Everolimus on these estrogen-deprived cells could help to explain the negative results of a recent randomized Phase 2 trial that added the aromatase inhibitor letrozole to Everolimus, they point out. (cancernetwork.com)

A phase I trial was conducted to evaluate the feasibility, safety, and efficacy of a dendritic cell-based vaccination in patients with metastatic renal cell carcinoma (RCC). (aacrjournals.org)
The poster presentation will highlight the first analysis of an allogeneic CD19 CAR T product composition to demonstrate that strategies intended to maximize stem central memory T-cell fraction (CCR7 + ) while limiting CD4 + CCR7 + differentiated fraction may improve safety and efficacy of CAR T therapy. (biospace.com)
THOUSAND OAKS, Calif. , June 8, 2016 /PRNewswire/ -- Amgen (NASDAQ:AMGN) today announced positive top-line results from the global Phase 2 study evaluating the efficacy and safety of erenumab (AMG 334) in chronic migraine prevention. (amgen.com)
The 20120295 study is a global Phase 2, randomized, 12-week, double-blind, placebo-controlled study evaluating the safety and efficacy of erenumab in chronic migraine prevention. (amgen.com)
While there are other methods of delivering genetic material into a cell, viruses have now been developed that demonstrate a good balance between efficacy and safety. (pall.com)
During phase II, the efficacy is tested on a small group of patients. (digitalhealthcentral.com)

We are also exploring the potential of our immune-evading stealth cell technology with PBCAR19B in earlier line DLBCL patients with the goal of displacing autologous CAR T treatment options. (biospace.com)

October 2, 2017 - Biohaven reported negative topline results from SCA phase 2/3 trial. (ataxia.org)
2017 saw a breakthrough in the treatment of primary progressive multiple sclerosis (MS) with ocrelizumab , a drug that depletes B cells. (medscape.com)

Dendritic cell vaccinations were done s.c. every 2 weeks for four times and repeated monthly until tumor progression. (aacrjournals.org)
After five dendritic cell injections, patients additionally received three injections weekly of low-dose interleukin-2 (1 million IE/m 2 ). (aacrjournals.org)

For the activation of CD4 + T-helper lymphocytes, dendritic cells were further incubated with the PAN-DR-binding peptide PADRE. (aacrjournals.org)
Such cells are known as "tumor infiltrating lymphocytes" (TILs). (biopharmconsortium.com)
He or she then expands the numbers of the antitumor T lymphocytes in cell culture, using the T-cell growth factor, IL-2. (biopharmconsortium.com)
We aimed to clarify whether soluble CD40 ligand (sCD40L) activated B cells may be loaded with HBcAg18-27 peptide and served as antigen-producing cells (APCs) to induce HBV-specific cytolytic T lymphocytes (CTLs). (biomedcentral.com)
Furthermore, we provide evidence that the activated B cells may serve as APCs to present core peptide of HBV to cytolytic T lymphocytes (CTLs). (biomedcentral.com)

New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
A main challenge of NK cell therapy is that it requires a high amount of functional NK cells. (frontiersin.org)
In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
Prior to founding Forge, Dr. Miller co-founded Abeona Therapeutics, a rare disease gene and cell therapy company. (xeromedia.com)
An evaluation of melanoma tissue from selected cases suggested a correlation between tumor-resident NY-ESO-1- and Melan-A-reactive T cells and disease control, supporting the notion of a therapy-associated sequestration of cells from the periphery to the tumor predominantly in those patients benefitting from ICB. (bmj.com)
The Novartis T-Charge platform is a next-generation CAR-T cell therapy manufacturing platform designed to preserve T cell stemness and facilitate CAR-T cell expansion primarily in vivo. (outsourcedpharma.com)
Dr Clive Glover is the Director of Cell & Gene Therapy at Pall. (pall.com)
Clive holds a PhD in Genetics and has over 15 years of experience in cell and gene therapy. (pall.com)
What is CAR-T cell therapy? (personalizemymedicine.com)
As its name implies, the backbone of CAR-T cell therapy is the T cell. (personalizemymedicine.com)
CAR-T cell therapy has shown promise for overcoming these challenges. (personalizemymedicine.com)

This is consistent with a negative-feedback loop, in which newly generated neurons modulate cell division of SVZ stem cells. (jneurosci.org)
The FDA has cleared America Stem Cell Inc. to proceed with its planned Phase I/IIa nationwide multi-center dual umbilical cord transplantation study to examine whether stem cells can effectively treat patients. (cellculturedish.com)
AIM OF THE STUDY: To investigate the effect of MA leaf and stem hydroethanolic extracts (LE and SE, respectively), their fractions enriched in triterpene acids or polyphenols as well isolated constituents, on CHIKV hosted in Vero cells. (bvsalud.org)

Cytometry and fluorescence microscopy showed that more than 98% sCD40L-activated B cells were loaded by the HBcAg peptide. (biomedcentral.com)
Furthermore, the peptide-pulsed activated B cells could induce HBcAg18-27 specific CTLs. (biomedcentral.com)

Therefore, ex vivo NK cell expansion protocols are currently being developed. (frontiersin.org)
In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
By directly injecting genetic material into the patient ( in vivo ), and where selected cells collected from the patient, undergo modification outside ( ex vivo ) before introducing them back into the patient. (pall.com)
Efficient antigen presentation by antigen presenting cells (APCs) is critical for inducing T-cell mediated immunity in vivo [ 1 , 2 ]. (biomedcentral.com)
More importantly, activated B cells may also prime naive T-cell responses against neoantigens ex vivo as DCs do [ 9 ]. (biomedcentral.com)
These compounds exhibited low cytotoxicity in Vero cells and an in in vivo animal model (Caenorhabditis elegans), indicating a favorable selectivity index. (bvsalud.org)

In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)

The number of viable cells was determined colorimetrically. (hindawi.com)
Is Novartis building a viable business model for adoptive immunotherapy for cancer? (biopharmconsortium.com)

They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
When administered to NHP, MDNA19 increases the absolute number of circulating CD8 T-cells in the absence of Treg and eosinophil stimulation (the latter being a major source of IL-5 production which is responsible for triggering vascular leak syndrome and associated toxicity). (newswire.ca)
In contrast, the PBMCs cultured in the absence of sCD40L contained no B cells analyzed by cytometry 20 days after culture (Figure 1d ). (biomedcentral.com)

Data and materials availability: Researchers may obtain HDM201 with a material transfer agreement from Novartis. (bace-signal.com)

To speed these drugs to market, Aditum Bio fosters an incubator model, focusing on the translational phase of drug development. (prnewswire.com)
Under the License Agreement, Legend Biotech will conduct a Phase 1 clinical trial for LB2102 in the U.S. Novartis will conduct all other development for the licensed products. (outsourcedpharma.com)
Cell Culture World Congress USA is North America's premier cell culture optimization and development conference, with a focus on upstream processes. (cellculturedish.com)
This is where biopharma and biotech biomanufacturing and process experts come to debate advances in cell culture optimization and development. (cellculturedish.com)

I just read on PNAS that the Novartis cancer drug Panobinostat (LBH-589), a Phase 3 drug developed for the treatment of cancer, may be able to halt the progression of Niemann Pick Type C disease, a fatal genetic cholesterol metabolism disorder that affects my twins. (addiandcassi.com)
The findings that HDAC inhibitors can correct cholesterol storage defects in human NPC1 mutant cells provide the potential basis for treatment options for NPC disease. (addiandcassi.com)
After success with that construct, they next looked for other exons that might be more sensitive to LMI070 by treating cultured cells with the drug and using high-throughput RNA sequencing to discover which genes were most actively expressed by the treatment. (scienceboard.net)
The primary endpoint was change in monthly migraine days from baseline to the last four weeks of the 12-week treatment phase in patients with chronic migraine (the number of migraine days between weeks 9 and 12). (amgen.com)
But they offer a compelling strategy for chasing down and eradicating ER+ cells, perhaps 'obviating the requirement for long-term endocrine treatment' in some patients. (cancernetwork.com)
We previously showed that the presence of NY-ESO-1-reactive and/or Melan-A-reactive T cells in the blood correlated with prolonged overall survival (OS) of patients with melanoma with a heterogeneous treatment background. (bmj.com)
This blog post, which was in part based on an article in the April 2011 issue of T he Scientist , described a treatment for metastatic melanoma known as adoptive cell transfer (ACT), or adoptive immunotherapy. (biopharmconsortium.com)
These Phase III data have been submitted to regulatory authorities in Europe and will be submitted to regulatory authorities in the United States this year, with the goal of bringing Cosentyx as a new treatment option to patients living with HS as soon as possible. (novartis.com)
Immunotherapy is a type of cancer treatment that makes use of a patient's own immune system to target and destroy cancer cells, with the aim of eliminating cancer from the body. (personalizemymedicine.com)
This form of treatment involves isolating cancer-targeting immune cells and expanding their numbers in the lab or endowing immune cells with the ability to recognize and kill cancer cells before transferring the modified immune cells back into the patient's body. (personalizemymedicine.com)

They include cell culture and histology lab, fluorescent microscopy (Leica hub), a bio-nanofabrication clean room, complex gate analysis platforms and high precision characterisation equipment. (move-upstream.org.uk)

In ACT, a physician/researcher extracts a patient's antigen-specific immune cells, which are usually found in tumor tissue. (biopharmconsortium.com)
We are very excited by the overwhelmingly positive data for MDNA19 following our pilot study in NHP which shows that, unlike competing programs, MDNA19 is able to dramatically boost cancer-killing immune cells for an extended duration without underlying safety issues," said Dr. Fahar Merchant , President and CEO, Medicenna. (newswire.ca)
This also helps to recruit other helper or killer immune cells such as B cells and NK cells to the site, which eventually results in the elimination of the infected cell, pathogen, or tumor. (personalizemymedicine.com)

Now comes an agreement (announced on August 6, 2012) between Novartis and the University of Pennsylvania (Penn) aimed at commercializing adoptive cellular immunotherapy. (biopharmconsortium.com)
So far, the technique of adoptive cell transfer (ACT) has been demonstrated to be the most effective cancer immunotherapy approach 1 . (personalizemymedicine.com)

However, direct injection of IL-2 has been shown to be accompanied by severe side effects, such as vascular leak syndrome, activation-induced cell death, and strong induction of regulatory CD4 pos T cells, which did not occur after IL-15 administration ( 15 , 16 ). (frontiersin.org)
A former cheerleader and direct others vascular outcomes (metronome walking many cultured blood glucose levels. (eventer.com)

Sphingosine 1-phosphate (S1P) is a bioactive lipid originating from cell membrane sphingolipids and associates with carrier molecules like albumin, ApoM, and ApoA4 to produce cellular effects. (eneuro.org)

Here, we investigated whether such reactive T cells can also be informative for clinical outcomes in metastatic melanoma under PD-1 immune-checkpoint blockade (ICB). (bmj.com)

Although traditionally associated with the last stages of the disease, recent findings with minimally transformed pretumorigenic primary human cells indicate that the ability to generate drug resistance arises early during the tumorigenic process, before the full transformation. (nature.com)
Adoptive immunotherapies lack a clearly defined claim to intellectual property (IP), since the patient's own cells are not a "drug" to be patented. (biopharmconsortium.com)

If a cancer center has a cell production facility with the required staff, the cost of producing a single dose of T-cells for adoptive transfer is approximately $20,000. (biopharmconsortium.com)

These studies demonstrated that a combination of three different osmolytes including sugar, sugar alcohol and amino acids/proteins could stabilize Jurkat cells and mesenchymal stromal cells (MSCs) during freezing. (nature.com)

We report the use of a series of small molecule histone deacetylase (HDAC) inhibitors in tissue culture models of NPC human fibroblasts. (addiandcassi.com)

Results from the Phase II trial are expected in the second half of 2018. (wikipedia.org)
Our results demonstrated high potential of IL-15 for NK cell expansion, while IL-21 triggered NK cell maturation and functionality. (frontiersin.org)
The T-Charge platform is designed to reduce the need for extensive culture time outside the body and results in T cells with greater proliferative potential, as well as fewer exhausted T cells. (outsourcedpharma.com)
These results demonstrate for the first time, that unlike peglylated versions of IL-2 which also tend to block activation of naïve CD8 T cells and NK cells, a long-acting IL-2 super-agonist can deliver best-in-class features without the complexity and limitations associated with other competing approaches. (newswire.ca)
Our results demonstrate that sCD40L-activated B cells may function as APCs and induce HBV-specific CTLs. (biomedcentral.com)

The studies presented in Breast Cancer Research involve tumor cell lines and biopsy samples, not women with breast cancer. (cancernetwork.com)
Two years ago, Matthew Ellis and the St. Louis team reported that inhibiting PI3K in combination with estrogen deprivation is a way to kill ER+ breast cancer cells, at least those growing in cell culture. (cancernetwork.com)
Now they have expanded these studies to include breast-cancer cell lines with differing mutations in the PI3K pathway (including PIK3CA) , and challenging them with 3 drugs that target the effects of PI3K. (cancernetwork.com)
Cancer rejection is mostly T cell-mediated. (bmj.com)
We are delighted that a combination of our unique candidate design in LB2102 with the T-Charge platform may potentially offer transformative benefits to small cell lung cancer patients. (outsourcedpharma.com)
[ii] Legend Biotech Announces FDA Clearance of IND Application for LB2102 in Extensive Stage Small Cell Lung Cancer. (outsourcedpharma.com)
For cancer regression to occur, the patient must have a repertoire of T cells that can recognize antigens expressed (displayed) by the tumor. (personalizemymedicine.com)

One recent article describes the use of hydrophilic solid phase extraction of glycyrrhizin (GA) in liquorice coupled with reversed-phase liquid chromatography purification, and although this method improved enrichment of GA, HILIC was only employed as a crude extraction procedure [9]. (chromatographytoday.com)
1998. Monitoring of pesticide residues and their metabolites in surface and underground waters of Imathia (N. Greece) by means of solid-phase extraction disks and gas chromatography. (cdc.gov)

Rivals are also developing medicines in the class of compounds known as BTK inhibitors, designed to selectively block the cells that drive the harmful autoimmune reaction behind MS. (pedfire.com)

In this study, we set up an effective culture method for long-term maintenance of B cells in vitro , in which the B cells are activated by human soluble CD40L (sCD40L). (biomedcentral.com)
A) Two trophozoites of T vaginalis obtained from in vitro culture, stained with Giemsa. (medscape.com)

Precision continues dosing subjects with optimized azer-cel CAR T cells in the CAR T relapsed population in which it has shown high and durable complete response rates, while further reducing the dose of lymphodepletion to standard levels in pursuit of best therapeutic index for this patient population. (biospace.com)
Precision continues to recruit patients at Dose Level 2 (flat dose of 540 million cells) with the intent to complete the Phase 1 dose escalation. (biospace.com)
Adding estrogen to the cell lines reduced this tumor-killing activity, but then pumping up the dose of the drugs progressively restored it. (cancernetwork.com)
Kinetic studies in NHP showed a dose-dependent upregulation of Ki67 in CD8 T-cells lasting for almost two weeks post-MDNA19 administration, with no apparent side effects. (newswire.ca)
Primary cultures of wild-type mouse astrocytes expressed S1P 1,2,3 transcripts, and selective deletion of S1P 1 and/or S1P 3 in cerebral cortical astrocytes, did not alter S1P-mediated, dose-dependent inhibition of glutamate uptake. (eneuro.org)

In this study, we addressed feeder cell-free expansion methods using common γ-chain cytokines, especially IL-15 and IL-21. (frontiersin.org)

The primary goal of the joint project is to develop better assays to evaluate the effectiveness of drugs in cell cultures and mice. (uga.edu)

The expression of CD80, CD86, major histocompatibility complex (MHC) classes I and II molecules on the sCD40L-activated B cell was significantly increased after long-time culture. (biomedcentral.com)
The CD40L-activated B cells may be continually expanded and the B cells significantly up-regulate the expression of major histocompatibility complex (MHC) class I and class II and induce the expression of CD80 and CD86. (biomedcentral.com)
FACS analyses showed that the percentage of B cells in the culture increased significantly over the time, and B cells accounted for about 80% of the total PBMCs when the cells were cultured for 54 days (Figure 1a and 1b ). (biomedcentral.com)

It is characterized by variable symptoms ranging from a minimal flulike illness to one that may be complicated by a toxic phase characterized by hemorrhage, hepatic failure, proteinuria, renal failure, and death. (medscape.com)

Anatomy2

Diseases2

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science2

Health Care1

Institutes for BioMedical Research4

Vaccines8

Therapies9

Pharma2

Collaboration6

20223

Proliferation6

Protein11

Tumors7

Immunology1

Gleevec1

Peripheral blood3

Mammalian2

Commercialization1

Antigen-specific T cells1

Pharmaceutical4

Preclinical2

20191

Trials13

Vaccine5

Apoptosis4

Receptor6

Clinical Development2

Inhibitor2

Efficacy6

Autologous1

20172

Dendritic cell2

Lymphocytes5

Therapy12

Stem3

Peptide2

Vivo8

Malignant cells2

Viable2

Absence3

Researchers1

Development4

Treatment10

Histology1

Immune cells3

Immunotherapy2

Vascular2

Membrane1

Outcomes1

Drug2

Adoptive transfer1

Stromal1

Tissue culture1

Results5

Cancer7

Solid phase extr2

Autoimmune1

Vitro2

Dose5

Methods1

Primary1

Significantly3

Renal1