• In addition, different parameters may affect the retinal circadian bioluminescence signal and its dynamic in in vitro assays. (molvis.org)
  • BioAssay Systems' method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. (gentaur.nl)
  • Simple, direct and high-throughput assays for total antioxidant capacity (TAC) find wide applications in research, food industry and drug discovery. (gentaur.fr)
  • Unused reconstituted NADH reagent is stable for three weeks when stored frozen at -20°C. Mix assay buffer well by vigorous shaking. (gentaur.fr)
  • 2. Prepare Working Reagent for Sample and Standard wells, by mixing for each well, 200 μL Assay Buffer, 1 μL Cofactor, 1 μL Enzyme Mix and 4 μL NADH. (gentaur.fr)
  • Warm to desired temperature (e.g. 37°C). Prepare Blank Reagent for the Blank well, by mixing 200 μL Assay Buffer, 1 μL Cofactor, 1 μL Enzyme Mix and 4 μL dH2O. (gentaur.fr)
  • For each Sample and Standard, prepare Working Reagent by mixing 1000 µL Assay Buffer, 5 µL Cofactor, 5 µL Enzyme Mix and 20 μL NADH. (gentaur.fr)
  • A membrane receptor reagent and assay is disclosed in which liposomes are bound to an evanescent wave emitting surface. (google.com)
  • Prepare enough Working Reagent by mixing, for each well, 85 µL Assay Buffer, 1 µL PLD Enzyme, 1 µL Enzyme Mix and 1 µL Dye Reagent. (gentaur.nl)
  • For samples that contain choline, prepare a blank control reagent with no PLD Enzyme (i.e., 85 µL Assay Buffer, 1 µL Enzyme Mix and 1 µL Dye Reagent). (gentaur.nl)
  • Prepare enough Working Reagent for Sample and Standard wells by mixing, for each assay well, 100 µL Reagent A and 8 µL Reagent B. Add 100 µL Working Reagent to all assay wells. (gentaur.fr)
  • His Gene research is multidisciplinary, incorporating perspectives in High-Throughput Screening Assays and Cell biology. (research.com)
  • Richard S. Paules focuses on Toxicity, Computational biology, Function, Prioritization and High-Throughput Screening Assays. (research.com)
  • His research in High-Throughput Screening Assays intersects with topics in Gene expression profiling, Biological pathway, Transcriptome, DNA microarray and Gene. (research.com)
  • Can be readily automated as a high-throughput assay for thousands of samples per day. (gentaur.fr)
  • In vitro cell-based assay development amenable to high-throughput biochemical screening, including stable cell line engineering, pluripotent stem cell differentiation, chaperone identification, and activity reporter technologies. (firmenich.com)
  • Prior to assay, bring the working reagents, microplate and spectrophotometer to the desired temperature. (gentaur.fr)
  • Choose from a comprehensive selection of instrumentation and reagents for your protein assay development from protein separation and confirmation in target discovery to protein profiling for biomarker studies. (bio-rad.com)
  • Explore options for the analysis of cells during assay development: instruments, reagents, and antibodies for cell counting, cell sorting, and flow cytometry analysis. (bio-rad.com)
  • If the calculated AST activity is higher than 100 U/L, dilute sample in Assay Buffer and repeat assay. (gentaur.fr)
  • if the calculated phospholipid concentration of a sample is higher than 200 µM in the Colorimetric Assay or 20 µM in the Fluorimetric Assay, dilute sample in 0.5% Triton X-100 and repeat the assay. (gentaur.nl)
  • if calculated TAC is higher than 1000 µM Trolox equivalents, dilute sample in dH2O and repeat assay. (gentaur.fr)
  • We would suggest that you dilute the urine 10-fold in water prior to the assay. (biotrend.com)
  • Biological analysis was carried out by collagen uterine contractility assays and pharmacological data were generated by competitive displacement binding and functional GPCR luciferase assays. (biomedcentral.com)
  • Prior to being appointed as head of DPTM, George was vice president of Quantitative Biosciences (QB) responsible for pre-clinical pharmacological assay development across disease areas and he led the implementation of new techniques and technologies to enable objective interrogation of biological systems to improve the prioritization of early-stage pipeline candidates. (phrmafoundation.org)
  • BioAssay Systems' AST activity assay is based on the quantification of oxaloacetate produced by AST. (gentaur.fr)
  • BioAssay Systems urea assay kit is designed to measure urea directly in biological samples without any pretreatment. (biotrend.com)
  • The technical methods and results of Matched Molecular Pair Analysis (MMPA) applied from a small, individual assay scale through large pharma scale, to multiple pharma data sharing scale have been published and reviewed. (slideshare.net)
  • In S. Martins & D. Prazeres, G Protein-Coupled Receptor Screening Assays - Methods in Molecular Biology (pp. 149-157). (uliege.be)
  • Complex pharmaceutical substances, preparations, or matter derived from organisms usually obtained by biological methods or assay. (bvsalud.org)
  • Biochemical and cellular pharmacology teams develop and validate assays to support research across drug discovery and development. (bio-rad.com)
  • Wang began working in the pharmaceutical industry in 2015, when he joined Janssen to develop biochemical and biophysical assays, which are fundamental tools in early-stage drug discovery. (acs.org)
  • The biological digitalis-like activity in the urine measured by the 86Rb-uptake assay was decreased, but not to a statistically significant degree. (lu.se)
  • I wonder if your urea assay kit could measure urea concentrations in dairy cow urine? (biotrend.com)
  • Yes, you can measure urea in cow urine with our urea assay. (biotrend.com)
  • Pharmacology & Therapeutics , 108217. (uliege.be)
  • Explore INDIGO's in vitro solutions in the broad categories of Prospective Screens, Safety Pharmacology, and Retrospective Screens, and start focusing your efforts on the best drug leads only. (indigobiosciences.com)
  • Target/Protein-based toxicity prediction (analogous to target-based drug discovery), which also forms the basis of eg safety pharmacology , and which is hence regularly employed in practice: You screen a compound against a dozen (or up to a hundred) proteins, and hope that the results will be informative. (drugdiscovery.net)
  • We used CCK-8 assay to determine the cell viability, used an optical microscope to measure cell morphology, Annexin-V/7-AAD kit to determine cell apoptosis, used Caspase 3/7 Activity Apoptosis Assay Kit to evaluate Caspase 3/7 activity, used Western blot to determine apoptosis-associated protein expression, and used flow cytometry and fluorescence microscope to determine the reactive oxygen species (ROS) levels and cell cycle. (neminis.org)
  • Direct Assays: AST activity in serum, plasma and other biological samples. (gentaur.fr)
  • Assay is compatible with serum or plasma (heparin, EDTA). (gentaur.fr)
  • phospholipid in biological samples such as serum and nonEDTA plasma. (gentaur.nl)
  • Liquid samples such as serum and plasma can be assayed directly. (gentaur.nl)
  • Liquid samples (e.g. nonhemolyzed serum, plasma) can be assayed directly. (gentaur.fr)
  • Unlike the other urea assays which measure ammonium after enzymatic conversion of urea to ammonium, this assay measures urea directly. (biotrend.com)
  • Enzymatic cleavage assays represent an attractive approach where the functional active toxins are detected by monitoring the cleavage of their target substrates, or peptide mimics in most cases, by means of different techniques, such as immunological detection 18 , 19 , capillary electrophoresis 20 , fluorescence analysis 21 , Forster resonance energy transfer (ALISSA) 22 , and high performance liquid chromatography 23 . (cdc.gov)
  • Nano-TiO2 also formed small agglomerates in medium containing as little as 1% protein and caused cellular interaction as measured by side scatter by flow cytometry and DNA damage (comet assay) in HepG2 cells. (cdc.gov)
  • Assays can be directly performed on raw biological samples i.e., in the presence of lipids and protein. (biotrend.com)
  • Nanoluciferase-based complementation assay to detect GPCR-G protein interaction. (uliege.be)
  • We perform syntheses, characterization, and preliminary biophysical and biological analysis ourselves. (fu-berlin.de)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • Effective use of hierarchical lines of evidence relies on consideration of the rele- vance and potency of assays, organization of assays by mode of action, and better assay validation. (cdc.gov)
  • It is standardized by a biological assay procedure. (druglib.com)
  • The fluorimetric assay procedure is similar to the colorimetric procedure except that (1) 0, 6, 12 and 20 µM phospholipid standards and (2) a black 96-well plate are used. (gentaur.nl)
  • Ordinarily, the relationship between changes in behavior of the indicator and differences in drug dose - (a dose-effect curve) - must be determined as a part of each assay. (pharmacologycorner.com)
  • With INDIGO's in vitro drug discovery assays, save resources and energy by identifying and characterizing adverse effects and potential risks before your drug candidate progresses further in the drug development process. (indigobiosciences.com)
  • Drug Discovery/Pharmacology: effects of drugs on AST activity. (gentaur.fr)
  • Modulation of tight junctions by peptides to increase drug penetration across biological barriers. (u-szeged.hu)
  • The design, validation, and implementation of assays that are sensitive and robust are vital for drug discovery, development, and testing during manufacturing. (bio-rad.com)
  • The types of assays and screens developed will depend on many factors such as the nature of a drug candidate and its putative action(s), the classes of the discovery and testing systems, and the manufacturing processes. (bio-rad.com)
  • Simple, direct, and automation-ready procedures for measuring urea concentration or blood urea nitrogen BUN in biological samples are becoming popular in Research and Drug Discovery. (biotrend.com)
  • With current in vitro screening assays now representing a $1.4B market with a predicted 12% annual growth rate, we believe we possess a product capable of significantly impacting the chemical/drug screening market and, here in particular, advancing our understanding of cytotoxic chemical biotransformations as they pertain to public health and consumer safety. (inknowvation.com)
  • The various areas that Richard S. Paules examines in his Gene expression study include Acetaminophen, Pharmacology, Immunology, Cell synchronization and Internal medicine. (research.com)
  • The MIC is essentially a cancer diagnostic chip that can generate single-cell 'molecular fingerprints' for a small quantity of pathology samples, including brain tumor tissues," said Dr. Hsian-Rong Tseng, a UCLA associate professor of molecular and medical pharmacology and one of the leaders of the research. (nanowerk.com)
  • Led by Tseng and Thomas Graeber, an assistant professor of molecular and medical pharmacology, both of whom are researchers at the Crump Institute for Molecular Imaging at the David Geffen School of Medicine at UCLA and the California NanoSystems Institute (CNSI) at UCLA, the team analyzed a panel of 19 human brain tumor biopsies to show the clinical application of the MIC platform to solid tumors. (nanowerk.com)
  • Molecular and medical pharmacology graduate researcher Michael Masterman-Smith approached the project as a translational cancer biologist. (nanowerk.com)
  • Microscale technology platforms are finding wide application in biological assays in which careful manipulation and measurement of limited sample amounts are required, and the new MIC platform is capable of making molecular measurements on small tumor samples provided by tumor resection and biopsy using as few as 1,000 to 3,000 cells, according to the researchers. (nanowerk.com)
  • He earned a B.A. in Biochemistry from Rutgers College, a Ph.D. in Chemistry from the University of Virginia and conducted postdoctoral research at Harvard Medical School in the Department of Biological Chemistry and Molecular Pharmacology. (phrmafoundation.org)
  • Practical and theoretical expertise in biochemistry, pharmacology, and cellular and molecular biology. (firmenich.com)
  • He was the very first person I recruited in a group of 40, and he was just amazing," says Kay Ahn, who was Janssen's global head of molecular and cellular pharmacology at the time. (acs.org)
  • If not assayed immediately, freeze supernatant at -80°C (stable for 1 month). (gentaur.fr)
  • For each assay plate, include two wells with 20 μL dH2O to be used for the NADH Standard and Blank. (gentaur.fr)
  • Warm to desired temperature (e.g. 37°C). To Blank control cuvette, add 960 µL Assay Buffer, 5 µL Cofactor, 5 µL Enzyme Mix and 20 μL dH2O. (gentaur.fr)
  • Solid samples can be homogenized in the assay buffer. (gentaur.nl)
  • Reconstitute Enzyme Mix with 120 µL Assay Buffer. (gentaur.nl)
  • In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. (gentaur.fr)
  • Evaluate our extensive range of available antibodies for use in assay development, such as ELISA, IHC, or FACS, or utilize our custom antibody services. (bio-rad.com)
  • Cellular interactions and biological responses to titanium dioxide nanoparticle s in HepG2 and BEAS-2B cells: role of cell culture media. (cdc.gov)
  • Furthermore, it adopted the revised guidance on good manufacturing practices for biological products, following the adoption of that guidance by the Expert Committee on Biological Standardization. (who.int)
  • 5 µM), sodium azide, EDTA, and sodium dodecyl sulfate are known to interfere in this assay and should be avoided in sample preparation. (gentaur.nl)
  • Ammonium does not interfere with the assay. (biotrend.com)
  • Linear detection range: colorimetric assay 3 - 200 µM, fluorimetric assay 0.6 - 20 µM phospholipid. (gentaur.nl)
  • Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in a 96-well plate assay. (biotrend.com)
  • This will give a final concentration of 12 to 17 mg/dL and is within the linear detection range of the assay (0 to 50 mg/dL). (biotrend.com)
  • For the advanced functional analysis and biological assays, we collaborate with the leading experts in the fields of infection biology, cell biology, pharmacology, and biophysics. (fu-berlin.de)
  • I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
  • You will develop solutions to complex biological and technical problems, and will collaborate with a diverse group of passionate and intellectually curious scientists. (firmenich.com)
  • By proximity extension assay (PEA), 182 proteins were measured in plasma and EVs from patients with AD (n = 10), Mild Cognitive Impairment (MCI, n = 10), and healthy controls (n = 10). (aau.dk)
  • Discover the many applications of our Droplet Digital™ PCR System for assay development when high sensitivity is required. (bio-rad.com)
  • As oxidative stress contributes to the development of many diseases including Alzheimer's disease, Parkinson's disease, diabetes, rheumatoid arthritis and neurodegeneration, the use of antioxidants in pharmacology is intensively studied. (gentaur.fr)
  • However, a number of assays are in development and the understanding of the biological mechanisms of chemical sensitization has greatly increased during the last decade. (lu.se)
  • NICEATM and NC3Rs are partnering with the National Institute of Allergy and Infectious Diseases, the U.S. Army Combat Capabilities Development Command Chemical Biological Center, and NCATS to direct the MPS for COVID Research (MPSCoRe) working group. (nih.gov)
  • assay development and provision of reference standards. (nibsc.org)
  • Our expertise lies in developing novel or refining existing in vitro analytical assays and we have contributed to the development of assays for several cytokines and understanding adverse effects of biological medicines such as TGN1412 . (nibsc.org)
  • Basic & Clinical Pharmacology & Toxicology , 115 (1), 110-117. (lu.se)
  • ADA monitoring of patients for clinical decision-making is gaining momentum, however measuring ADAs is challenging and efforts to standardise such assays are recognised as an important step towards ensuring comparability of results. (nibsc.org)
  • By combining the results from two-stage quantification, 4 or 5 orders of magnitude in dynamic range were achieved for the detection of the serotypes of BoNT/A, /B, /E, or /F. The effect of multiplexing the assay by mixing substrates for different BoNT serotypes into a single reaction was also investigated in order to reduce the numbers of the cleavage reactions and to save valuable clinical samples. (cdc.gov)
  • The impact of Oncotype DX breast cancer assay results on clinical practice: a UK experience. (cdc.gov)
  • When the absolute amounts of standard used in the assay are known, the results of the assay can be used to estimate the amount - in absolute units - of biologically active material contained in the unknown preparation. (pharmacologycorner.com)
  • Hi everyone, I have a question about the error on certain biological results. (physicsforums.com)
  • Browse our large library of disease- and pathway-specific predesigned PCR primers, probes, and assay panels that meet MIQE guidelines or order custom-designed primers and plates. (bio-rad.com)
  • Both of those aspects seem superficially mirror images of each other, but far from it - efficacy is the presence of one desirable property , of which there may be one or more which are leading to the desired effect, such as up- or downregulating a particular biological pathway (or many other things). (drugdiscovery.net)
  • The biological indicators in bioassay are the reactions of living organisms or tissues. (pharmacologycorner.com)
  • EVs provided additional biological information related to AD not observed in plasma alone. (aau.dk)
  • Our understanding of `magic' mushroom pharmacology has been limited by access to pure, well-characterized chemicals for biological assays. (iucr.org)
  • Toxicokinetics and Cardiotoxicity is closely connected to Pharmacology in his research, which is encompassed under the umbrella topic of Toxicity. (research.com)
  • His Toxicity research includes elements of Zebrafish and Pharmacology. (research.com)
  • Can the 'standard deviation' or the assay itself be calculated from these data, as if they were direct, repeated measurements? (physicsforums.com)
  • The utility of AOPs for regulatory application is defined, to a large extent, by the confidence and precision with which they facilitate extrapolation of data measured at low levels of biological organisation to predicted outcomes at higher levels of organisation and the extent to which they can link biological effect measurements to their specific causes. (aopwiki.org)
  • We propose that integrated testing strategies should comprise complementary assays, providing measurements of a wide range of mechanistic events, to perform well-educated risk assessments based on weight of evidence. (lu.se)
  • Latin or common names of a species or broader taxonomic grouping (e.g., class, order, family) that help to define the biological applicability domain of the KER.In general, this will be dictated by the more restrictive of the two KEs being linked together by the KER. (aopwiki.org)
  • Toxicity profiling of flame retardants in zebrafish embryos using a battery of assays for developmental toxicity, neurotoxicity, cardiotoxicity and hepatotoxicity toward human relevance. (research.com)
  • Furthermore, by leveraging human cells as the sensing platform, our assay provides more accurate and realistic information in regards to bioavailability and a chemical's true effect on individual human health than does the employment of small animal models. (inknowvation.com)
  • Streamline gene expression assays with our automated qPCR systems. (bio-rad.com)