2021Ubiquitin ligase activityProteinEnzymesEpubMutations2020EnzymologySystematicProteinsITCHBiochemicalTubulinActivatesAssaysAmideParkinUbiquitinationSubstrateNuclearBelongsSyndromeUbiquitin ligase complexCharacterizationRegulatesCarbon-nitrogen bondsSubstratesEnzymeMolecularDegradationHistoneExpressionARIP3UniProtCellsCullinSubunitRatsComplexInhibitorStructural
20211
- In animals, 5mC is maintained during DNA replication by DNMT1 together with UHRF1, which directly recognizes hemimethylated cytosine via the SRA domain and stimulates activity of DNMT1 in a manner dependent on its ubiquitin-ligase activity ( Nishiyama and Nakanishi, 2021 ). (elifesciences.org)
Ubiquitin ligase activity2
- PINK1 phosphorylates ubiquitin to activate Parkin E3 ubiquitin ligase activity. (bioseek.eu)
- Recombinant TcPINK1 directly phosphorylated ubiquitin and phospho-ubiquitin activated Parkin E3 ubiquitin ligase activity in cell-free assays. (bioseek.eu)
Protein10
- This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. (nih.gov)
- Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals. (nih.gov)
- lister encodes a RING finger protein, LISTERIN, which functions as an E3 ubiquitin ligase in vitro. (nih.gov)
- We identify the SUMO ligase Protein Inhibitor of Activated STAT1 (PIAS1) as a constituent PML-NB protein. (gla.ac.uk)
- Link to all annotated objects annotated to protein-glycine ligase activity. (planteome.org)
- Link to all direct and indirect annotations to protein-glycine ligase activity. (planteome.org)
- A protein complex that includes a ubiquitin-protein ligase and enables ubiquitin protein ligase activity. (planteome.org)
- Morris, T.W., Reed, K.E. and Cronan, J.E., Jr. Identification of the gene encoding lipoate-protein ligase A of Escherichia coli . (enzyme-database.org)
- p53 E3 ubiquitin protein ligase homolog (Mdm2) is an important negative regulator of p53. (molvis.org)
- The quest for novel targeted protein degraders starts with discovery - finding and evaluating molecules that interact with both the target protein and the cellular degradation machinery, such as the E3 ligase pathway (Lee et al. (biognosys.com)
Enzymes1
- Considering the diversity of microtubule polyglutamylation and the existence of many non-tubulin substrates, it is important to understand how the effector enzymes, the tubulin ligase-like (TTLL) proteins, are regulated. (wjgnet.com)
Epub2
- Epub ahead of print] PMID: 23412628. (wayne.edu)
- Epub ahead of print] PubMed PMID: 34847973. (ydklab.org)
Mutations4
- Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance. (nih.gov)
- Ubiquitin ligases of the N-end rule pathway: assessment of mutations in UBR1 that cause the Johanson-Blizzard syndrome. (yeastgenome.org)
- A severe form of human combined immunodeficiency due to mutations in DNA ligase IV. (ac.ir)
- PMID:18786442 mutations and the risk of esophageal squamous cell carcinoma. (who.int)
20201
Enzymology2
- In enzymology, a 4-methyleneglutamate-ammonia ligase (EC 6.3.1.7) is an enzyme that catalyzes the chemical reaction ATP + 4-methylene-L-glutamate + NH3 ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + 4-methylene-L-glutamine The 3 substrates of this enzyme are ATP, 4-methylene-L-glutamate, and NH3, whereas its 3 products are AMP, diphosphate, and 4-methylene-L-glutamine. (wikipedia.org)
- In enzymology, a phenylacetate-CoA ligase is an enzyme (EC 6.2.1.30) that catalyzes the chemical reaction ATP + phenylacetate + CoA ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + phenylacetyl-CoA The 3 substrates of this enzyme are ATP, phenylacetate, and CoA. (wikipedia.org)
Systematic2
- The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). (wikipedia.org)
- The systematic name of this enzyme class is phenylacetate:CoA ligase (AMP-forming). (wikipedia.org)
Proteins1
- This is facilitated by proteins, called ligases, which were discovered in the 1960's. (vectorbuilder.cn)
ITCH2
Biochemical1
- Purification and biochemical characterization of phenylacetyl-CoA ligase from Pseudomonas putida. (wikipedia.org)
Tubulin1
- Melo-Hanchuk TD, Kobarg J. Polyglutamylase activity of tubulin tyrosine ligase-like 4 is negatively regulated by the never in mitosis gene A family kinase never in mitosis gene A -related kinase 5. (wjgnet.com)
Activates3
- The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome. (neurotree.org)
- PINK1 kinase activates the E3 ubiquitin ligase Parkin to induce selective autophagy of damaged mitochondria. (bioseek.eu)
- PINK1 phosphorylates ubiquitin, which then binds to Parkin and activates its E3 ligase activity, leading to induction of selective autophagy of damaged mitochondria. (bioseek.eu)
Assays1
- PMID:19699081 assays, segregation, and tumor characteristics. (who.int)
Amide1
- This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). (wikipedia.org)
Parkin1
- These results explain a feed-forward mechanism of PINK1-mediated initiation of Parkin E3 ligase activity. (bioseek.eu)
Ubiquitination2
- CHIP is an E3 ligase, recognizing Hsp70-bound substrates via the TPR binding domain and catalyzing their ubiquitination via the U-box domain (hexagon). (elifesciences.org)
- Hsp70 acts as a fine-switch that controls E3 ligase CHIP-mediated TAp63 and ΔNp63 ubiquitination and degradation. (florescancerlab.org)
Substrate1
- C ) Schematic for engineering an E3 ubiquitin ligase and potential outcomes on an ion channel substrate. (elifesciences.org)
Nuclear1
Belongs1
- This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. (wikipedia.org)
Syndrome1
- Ligase IV syndrome. (ac.ir)
Ubiquitin ligase complex5
- A ubiquitin ligase complex. (planteome.org)
- Link to all annotated objects annotated to Fused-Smurf ubiquitin ligase complex. (planteome.org)
- Link to all direct and indirect annotations to Fused-Smurf ubiquitin ligase complex. (planteome.org)
- Proteolysis Targeting Chimaeras (PROTAC) are hetero-bifunctional molecules which incorporate a known binding moiety to the protein of interest (POI, e.g. an inhibitor), coupled to a ligand for an E3 ubiquitin ligase complex. (le.ac.uk)
- Hepatitis B Virus HBx Protein Mediates the Degradation of Host Restriction Factors through the Cullin 4 DDB1 E3 Ubiquitin Ligase Complex. (nih.gov)
Characterization4
- Purification and biochemical characterization of phenylacetyl-CoA ligase from Pseudomonas putida. (wikipedia.org)
- Archaeal 3'-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation. (nih.gov)
- Callis, J. Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis. (benthamscience.com)
- Purification and Characterization of Human DNA Ligase IIIa Complexes After Expression in Insect Cells. (jefferson.edu)
Regulates3
- Kowalski JR , Dube H, Touroutine D , Rush KM, Goodwin PR , Carozza M, Didier Z, Francis MM , Juo P . The Anaphase-Promoting Complex (APC) ubiquitin ligase regulates GABA transmission at the C. elegans neuromuscular junction. (neurotree.org)
- Yonezawa, T., The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1. (riken.jp)
- Ubiquitin-specific protease 19 regulates the stability of the E3 ubiquitin ligase MARCH6. (titech.ac.jp)
Carbon-nitrogen bonds1
- This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia (or amine) ligases (amide synthases). (wikipedia.org)
Substrates3
- In enzymology, a 4-methyleneglutamate-ammonia ligase (EC 6.3.1.7) is an enzyme that catalyzes the chemical reaction ATP + 4-methylene-L-glutamate + NH3 ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + 4-methylene-L-glutamine The 3 substrates of this enzyme are ATP, 4-methylene-L-glutamate, and NH3, whereas its 3 products are AMP, diphosphate, and 4-methylene-L-glutamine. (wikipedia.org)
- In enzymology, a phenylacetate-CoA ligase is an enzyme (EC 6.2.1.30) that catalyzes the chemical reaction ATP + phenylacetate + CoA ⇌ {\displaystyle \rightleftharpoons } AMP + diphosphate + phenylacetyl-CoA The 3 substrates of this enzyme are ATP, phenylacetate, and CoA. (wikipedia.org)
- BTB, Cul3 and RBX1 form a Cul3-based ligase (BCR), which targets substrates for ubiquitin-dependent degradation by the 26S proteasome. (biocuckoo.org)
Enzyme4
- The systematic name of this enzyme class is 4-methylene-L-glutamate:ammonia ligase (AMP-forming). (wikipedia.org)
- This enzyme belongs to the family of ligases, specifically those forming carbon-sulfur bonds as acid-thiol ligases. (wikipedia.org)
- The systematic name of this enzyme class is phenylacetate:CoA ligase (AMP-forming). (wikipedia.org)
- ywfE in Bacillus subtilis codes for a novel enzyme, L-amino acid ligase. (kegg.jp)
Molecular1
- Molecular architecture of the human tRNA ligase complex. (nih.gov)
Degradation2
- Ltn1 is a 180-kDa E3 ubiquitin ligase that associates with ribosomes and marks certain aberrant, translationally arrested nascent polypeptide chains for proteasomal degradation. (nih.gov)
- Direct recruitment of the E3 ligase to the POI via the PROTAC, targets it for ubiquitination and ultimately degradation. (le.ac.uk)
Histone1
- Data indicate the mechanism for epigenetic regulation in cancer by inducing E3 ubiquitin ligase NEDD4-dependent histone H3 ubiquitination. (cusabio.com)
Expression2
- Human and viral membrane-associated E3 ubiquitin ligases MARCH1 and MIR2 recognize different features of CD86 to downregulate surface expression. (edu.au)
- Low expression of the E3 ubiquitin ligase CBL confers chemoresistance in human pancreatic cancer and is targeted by epidermal growth factor receptor inhibition. (uclahealth.org)
ARIP31
- As the functional regulators of FLI-1 are largely unknown, we performed a yeast two-hybrid screen with FLI-1 and identified the SUMO E3 ligase PIASxalpha/ARIP3 as a novel in vitro and in vivo binding partner of FLI-1. (pasteur.fr)
UniProt1
- An example of this is FUSED in Drosophila melanogaster (UniProt symbol P23647) in PMID:21145463 (inferred from direct assay). (planteome.org)
Cells1
Cullin1
- Two-dimensional classifications and subsequent 3D reconstructions of electron density maps show that Ltn1 has an elongated form and presents a continuum of conformational states about two flexible hinge regions, whereas its overall architecture is reminiscent of multisubunit cullin-RING ubiquitin ligase complexes. (nih.gov)
Subunit1
- As a substrate recognition subunit of Cul3-based ligase, the member of BTB family can bridge the Cul3 to the substrate in a single polypeptide (2,3). (biocuckoo.org)
Rats1
- MARCH7 E3 ubiquitin ligase is highly expressed in developing spermatids of rats and its possible involvement in head and tail formation. (titech.ac.jp)
Complex1
- Part of tRNA-splicing ligase complex. (nih.gov)
Inhibitor1
- Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase. (mshri.on.ca)
Structural1
- We present a review of the recent progress toward the structural understanding of E3 ligases. (nih.gov)