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Protein Inhibitors of Activated STATSTAT3 Transcription FactorSTAT1 Transcription FactorSTAT5 Transcription FactorSmall Ubiquitin-Related Modifier Proteins5-Lipoxygenase-Activating Protein InhibitorsSUMO-1 ProteinSumoylationDictionaries as TopicSTAT6 Transcription FactorProteinsSteatorrheaSignal TransductionSTAT2 Transcription FactorSTAT4 Transcription FactorTrans-ActivatorsMolecular Sequence DataProtein BindingDNA-Binding ProteinsSTAT Transcription FactorsEleusineAmino Acid SequenceDatabases, GeneticMEDLINETwo-Hybrid System TechniquesAbstracting and Indexing as TopicPubMedGene Expression RegulationNatural Language ProcessingCholesterol Ester Transfer ProteinsTranscription, GeneticTerminology as TopicCell LineQuinolinesInformation Storage and RetrievalBlotting, WesternGenesTranscription FactorsTransfectionData MiningCell Line, TumorTenebrioReceptors, ComplementCells, CulturedMilk ProteinsProtein Interaction MappingPhosphorylationInternetCarrier ProteinsSoftwareMolecular ChaperonesRNA, MessengerComputational BiologyBase SequenceGene Expression ProfilingCystatinsStreptomycetaceaeEnzyme InhibitorsTranscriptional ActivationProtein Structure, TertiaryDatabases, ProteinProtease InhibitorsIntracellular Signaling Peptides and ProteinsVocabulary, ControlledTrypsin InhibitorsOvomucinBinding SitesGene Regulatory NetworksGene ExpressionCell NucleusImmunohistochemistryMice, Inbred C57BLCOS CellsDatabase Management Systems5-Lipoxygenase-Activating ProteinsReverse Transcriptase Polymerase Chain ReactionUser-Computer InterfaceGenomicsLipoxygenase InhibitorsSerine Proteinase InhibitorsMutationJanus Kinase 2Janus KinasesUbiquitin-Protein LigasesProteomicsOligonucleotide Array Sequence AnalysisSuppressor of Cytokine Signaling ProteinsModels, MolecularKineticsApolipoprotein C-IAnticholesteremic Agentsalpha-AmylasesOxazolidinonesProtein Phosphatase 1Sequence Homology, Amino AcidEnzyme ActivationGene Expression Regulation, NeoplasticNitric Oxide Synthase Type IArtificial IntelligenceThiadiazoles
STAT3SumoylationELISA KitSignaling moleculesMediatePIASMass spectrometryPathwaysTyrosineSubsequentlyTranscriptionalModulatePhosphorylationActivator of transcriptionAntibodyReceptorVariantsInteractionsCytokinesAntibodiesReagents distributedProgressionRegulateSubstratesProtectiveHumansBindMechanismsSpecificStrainLeukocytesHumanExpressionCellularResponsesInflammationTargetRoleCellFamilyCancer
STAT316
  • Originally, PIAS3 was found to interact specifically with phosphorylated STAT3 in Interleukin -6 (IL-6) activated murine myeloblast M1 cells. (wikipedia.org)
  • This interaction is mediated via PIAS3 binding to the STAT3 DNA binding domain. (wikipedia.org)
  • Hence, STAT3 transcriptional activity is inhibited by the physical prevention of its binding to target genes. (wikipedia.org)
  • Additionally, STATT can recruit protein phosphatases to dephosphorylate and inactivate STAT3, further attenuating its activity. (creativebiomart.net)
  • STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. (thermofisher.com)
  • Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. (thermofisher.com)
  • STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. (thermofisher.com)
  • In humans, the STAT3 gene is located on the q arm of chromosome 17. (thermofisher.com)
  • STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. (thermofisher.com)
  • The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). (thermofisher.com)
  • The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. (thermofisher.com)
  • Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage. (thermofisher.com)
  • Leptin-activated leptin receptor (LepRb) signals in hypothalamus via several pathways, in which JAK2-STAT3 pathway, the most extensively investigated one, is considered to mediate the major action of leptin in energy regulation. (biomedcentral.com)
  • Phosphorylated STAT3 (p-STAT3), as a transcription factor, binds to and regulates its target gene such as POMC gene, playing the physiological function of leptin. (biomedcentral.com)
  • Regarding POMC gene in hypothalamus however little is known about the detail of its interaction with STAT3. (biomedcentral.com)
  • Targeted disruption of the mouse Stat3 gene leads to early embryonic lethality. (sagepub.com)
Sumoylation5
  • These regulatory proteins also modulate the DNA-binding properties of STATs by facilitating SUMOylation of the dimers. (creativebiomart.net)
  • SUMOylation is a post-translational modification of proteins that has been found to play a major role in the Wnt/β-catenin signaling pathway. (frontiersin.org)
  • Post-translational modifications (PTMs) of proteins, including phosphorylation, acetylation, ubiquitination, and SUMOylation, can regulate the function of proteins, determine the active state and subcellular location of proteins, and dynamically interact with other proteins related to carcinogenesis and progression ( 17 - 20 ). (frontiersin.org)
  • SUMOylation of proteins is an important mechanism in cellular responses to environmental stress ( 21 , 22 ). (frontiersin.org)
  • Proteins associated with the Wnt/β-catenin pathway have been identified as SUMOylated substrates, and evidences suggested that the initiation and progression of cancers depended on the function of the SUMOylation ( 23 ). (frontiersin.org)
ELISA Kit2
  • Description: A sandwich ELISA kit for detection of GRB2 Related Adaptor Protein 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (therabio.org)
  • Description: A sandwich ELISA kit for detection of G Protein Coupled Receptor 172A from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (myelisakit.com)
Signaling molecules1
  • SOCS proteins, including SOCS1, SOCS2, SOCS3, and CIS, bind to activated Jaks and cytokine receptors, leading to their degradation or blocking the recruitment of downstream signaling molecules. (creativebiomart.net)
Mediate1
  • Although cytokines are known to activate multiple signaling pathways that together mediate these important functions, one of these pathways, the Jak-STAT pathway, is the focus of this chapter. (sagepub.com)
PIAS10
  • The mammalian PIAS family consists of four members: PIAS1, PIAS2, PIAS3 and PIAS4. (wikipedia.org)
  • In yeast, two PIAS-related proteins were identified namely SIZ1 and SIZ2. (wikipedia.org)
  • The PIAS family contains more than 60 proteins, most of them transcription factors that can be either positively or negatively regulated through multiple mechanisms. (wikipedia.org)
  • The SAF-A/B, Acinus and PIAS (SAP) domain is located at the N-terminal of PIAS proteins. (wikipedia.org)
  • An LXXLL signature motif is present within the SAP domain of all PIAS proteins. (wikipedia.org)
  • The Pro-Ile-Asn-Ile-Thr (PINIT) motif represents a highly conserved region of PIAS proteins, which was shown to be involved in the nuclear retention of PIAS3. (wikipedia.org)
  • The RING-finger-like zinc-binding domain (RLD) is one of the most conserved domains of the PIAS family and has been shown to be important for PIAS3 activity as a SUMO-E3 ligase. (wikipedia.org)
  • PIAS proteins interact with activated STAT dimers and inhibit STAT-mediated transcription. (creativebiomart.net)
  • PIAS proteins are another class of negative regulators that exert their inhibitory effects by directly interacting with activated STATs. (creativebiomart.net)
  • Additionally, PIAS proteins can also recruit corepressors and histone deacetylases to inhibit STAT-mediated gene expression. (creativebiomart.net)
Mass spectrometry2
  • Using LCâ MS/MS mass spectrometry and coimmunoprecipitation assays, we identified vimentin as a host protein that differentially interacted with prototypic and mutant HN proteins. (bvsalud.org)
  • Chemical cross-linking of proteins with mass spectrometry (XL-MS) is a rapidly developing structural biology technique able to provide valuable insight into protein conformations and interactions, even as they exist within their native cellular environment. (tuberculosistest.net)
Pathways1
  • Cytokines induce a variety of biological responses by binding to specific cell surface receptors and activating cytoplasmic signaling pathways (e.g., the JAK/STAT pathway). (creativebiomart.net)
Tyrosine4
  • Key negative regulators of this pathway include Suppressors of Cytokine Signaling (SOCS), Protein Inhibitors of Activated STATs (PIASs), and protein tyrosine phosphatases (PTPs). (creativebiomart.net)
  • Jak/STAT signaling is also regulated by numerous constitutively expressed PTPs that dephosphorylate important tyrosine residues on multiple components of the pathway, thereby attenuating Jak/STAT signaling. (creativebiomart.net)
  • The pathway uses a novel mechanism in which cytosolic latent transcription factors, known as signal transducers and activators of transcription (STATs), are tyrosine phosphorylated by Janus family tyrosine kinases (Jaks), allowing STAT protein dimerization and nuclear translocation. (sagepub.com)
  • Molecular cloning of L-JAK, a Janus family protein-tyrosine kinase expressed in natural killer cells and activated leukocytes. (sagepub.com)
Subsequently1
Transcriptional1
  • These elements are frequently found near gene enhancers and interact with nuclear matrix proteins to provide a unique nuclear microenvironment for transcriptional regulation. (wikipedia.org)
Modulate1
Phosphorylation1
  • This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. (cd-34.com)
Activator of transcription1
  • Studies in the past decade have demonstrated the essential roles of several AJC protein, including underlying actinomyosin components, actin isoforms, and intracellular signal transducer and activator of transcription (STAT) transcription factors, and nuclear factor-κB (NF-κB) in the maintenance and remission of IBD progression ( 5 ). (frontiersin.org)
Antibody6
  • Human IgG antibody Laboratories manufactures the dna stat in texas reagents distributed by Genprice. (cd-34.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. (therabio.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GA Binding Protein Transcription Factor Alpha (GABPa) in samples from serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human GRB2 Related Adaptor Protein 2 (GRAP2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (therabio.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human G Protein Coupled Receptor 172A (GPR172A) in Tissue homogenates, saliva and other biological fluids. (myelisakit.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat High Density Lipoprotein Binding Protein (HDLBP) in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (tuberculosistest.net)
Receptor4
  • It is also essential for the binding of PIAS3 to androgen receptor. (wikipedia.org)
  • In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. (cd-34.com)
  • Signaling is initiated when the Wnt ligand binds to the Frizzled receptor on the cell membrane and the LDL receptor-associated protein 5/6 (LRP5/6) co-receptor. (frontiersin.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human G Protein Coupled Receptor 120 (GPR120) in samples from tissue homogenates, cell lysates or other biological fluids. (myelisakit.com)
Variants1
  • Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. (wikipedia.org)
Interactions1
  • The study of protein structures and interactions is critical to understand their function. (tuberculosistest.net)
Cytokines1
  • Following exposure to pathogens, the innate immune cells of gastrointestinal mucosa release pro-inflammatory cytokines and activate an adaptive immune response to aggravating inflammation in the intestine ( 3 ). (frontiersin.org)
Antibodies3
  • Creative BioMart offers a wide range of products and services related to Jak/STAT pathway research, including recombinant proteins, antibodies, and assay kits, to facilitate the study of these negative regulators and their potential applications in various fields. (creativebiomart.net)
  • Security and Tolerability of three CGRP Monoclonal Antibodies in Apply: A Retrospective Cohort Examine Goal: We sought to evaluate the protection and tolerability of three calcitonin gene-related peptide (CGRP) monoclonal antibodies in sufferers with power migraine who have failed a number of lessons of migraine preventive therapies. (tuberculosistest.net)
  • Complete Evaluation of the Antigenic Influence of Human Papillomavirus Lineage Variation on Recognition by Neutralizing Monoclonal Antibodies Raised Towards Lineage A Main Capsid Proteins of Vaccine-Associated Genotypes Human papillomavirus (HPV) is the causative agent of cervical and different epithelial cancers. (tuberculosistest.net)
Reagents distributed1
  • Awarness Stat Fax Laboratories manufactures the technological awareness reagents distributed by Genprice. (taueret.com)
Progression1
  • INTRODUCTION: Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) is involved in glioma progression, but the specific molecular mechanism of CDKN2A in glioma cell migration and invasion needs to be further explored. (bvsalud.org)
Regulate1
  • The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. (cd-34.com)
Substrates2
  • PIAS3 protein also functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. (wikipedia.org)
  • They act as pseudo-Jak substrates to inhibit Jak/STAT signaling. (creativebiomart.net)
Protective3
  • Innate immunity in gastrointestinal system includes epithelial cell layers that express tight cell-cell contact, secreted mucous layer which prevent entry of microbes, protective plasma protein, and circulating leukocytes, such as phagocytic macrophages, neutrophils, dendritic cells (DC), natural killer (NK) cells, NK T-cell, and innate lymphoid cells. (frontiersin.org)
  • The aim of our study was to conduct a comparative analysis of the expression levels of cytoskeletal proteins (glial fibrillar acidic protein (GFAP) and vimentin), protective protein S100, and proapoptotic caspase-3 protein in patients with drug-resistant epilepsy (DRE) associated with focal cortical dysplasia (FCD). (bvsalud.org)
  • In the early stages of disease development, there was a compensatory sequential increase in the expression of cytoskeletal and protective proteins. (bvsalud.org)
Humans1
  • E3 SUMO-protein ligase PIAS3 is an enzyme that in humans is encoded by the PIAS3 gene. (wikipedia.org)
Bind1
  • This evolutionarily conserved domain is found in proteins ranging from yeast to human and is shared by other chromatin-binding proteins, such as scaffold attachment factor A and B. The SAP domain can recognize and bind to AT-rich DNA sequences present in scaffold-attachment regions/matrix-attachment regions. (wikipedia.org)
Mechanisms3
  • Signaling through the Jak/STAT pathway is tightly controlled by several distinct mechanisms as dysregulation of Jak/STAT signaling is thought to underlie several disorders including rheumatologic and autoimmune disorders, transplant rejection, and several cancers. (creativebiomart.net)
  • Negative regulators employ diverse mechanisms to constrain the Jak/STAT pathway. (creativebiomart.net)
  • Understanding their mechanisms and functions provides valuable insights for therapeutic interventions aimed at modulating the Jak/STAT signaling pathway. (creativebiomart.net)
Specific1
  • PIAS3 protein is a specific inhibitor of this protein. (cd-34.com)
Strain1
  • In a previous study, the mutant HN protein was identified as a crucial virulence factor for the velogenic variant NDV strain JS/7/05/Ch, which evolved from the prototypic vaccine strain Mukteswar. (bvsalud.org)
Leukocytes1
  • The innate immune response also includes complement proteins, defensins, and chemokines which attract inflammatory leukocytes, lipid mediator of inflammation, bioactive amines, and enzymes to enhance inflammation ( 2 ). (frontiersin.org)
Human1
  • The Dna Stat In Texas reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (cd-34.com)
Expression3
  • This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. (cd-34.com)
  • We aimed to investigate how the expression levels of these proteins depend on age (both in children and adults), gender, and disease duration, using immunohistochemistry. (bvsalud.org)
  • In the epileptic focus area of the cortex and white matter in patients with FCD-associated temporal lobe DRE, a higher level of expression of these proteins was observed. (bvsalud.org)
Cellular1
  • Negative regulators of the Jak/STAT pathway are essential for maintaining proper cellular homeostasis and preventing pathological conditions. (creativebiomart.net)
Responses1
  • Negative regulators of the Jak/STAT pathway play a critical role in modulating immune responses. (creativebiomart.net)
Inflammation1
  • By attenuating the Jak/STAT signaling, they prevent excessive inflammation and immune cell activation. (creativebiomart.net)
Target1
  • They also block STAT activation and target multiple components of the Jak/STAT pathway for Ubiquitin-mediated proteasomal degradation. (creativebiomart.net)
Role3
  • The haemagglutinin-neuraminidase (HN) protein plays a crucial role in the infectivity and virulence of Newcastle disease virus (NDV). (bvsalud.org)
  • Herein, we elucidate the crucial role of vimentin, an intermediate filament protein, in regulating NDV infectivity through targeting of the HN protein. (bvsalud.org)
  • Abnormally activated Wnt/β-catenin signaling pathway plays a crucial role in promoting malignant tumor occurrence, development, recurrence, and metastasis, particularly in cancer stem cells. (frontiersin.org)
Cell3
  • The Jak/STAT pathway is involved in cell differentiation and development. (creativebiomart.net)
  • Selective permeability of IECs is achieved by apical junctional complex (AJC) proteins which comprise tight junctions, adherens junctions, and desmosomes to maintain the apicobasal cell polarity and intestinal tissue integrity ( 4 ). (frontiersin.org)
  • Distinct tumorigenic potential of abl and raf in B cell neoplasia: abl activates the IL-6 signaling pathway. (sagepub.com)
Family2
  • Description: The protein encoded by this gene is a member of the STAT protein family. (cd-34.com)
  • 简介 The protein encoded by this gene is a member of the STAT protein family. (solarbio.cc)
Cancer1
  • Dysregulation of the Jak/STAT pathway is often associated with cancer development. (creativebiomart.net)