Pc12 cells. Medical search. Frequent questions

PC12 cells were used to find which prion protein fragments caused neuronal dysfunction. (wikipedia.org)

It was developed in parallel to the adrenal chromaffin cell model because of its extreme versatility for pharmacological manipulation, ease of culture, and the large amount of information on their proliferation and differentiation. (wikipedia.org)
This makes PC12 cells useful as a model system for neuronal differentiation and neurosecretion. (wikipedia.org)
PC12 cells stop dividing and undergo terminal differentiation when treated with NERVE GROWTH FACTOR , making the line a useful model system for NERVE CELL differentiation . (online-medical-dictionary.org)
ASK1 thus appears to mediate signals leading to both differentiation and survival of PC12 cells. (nih.gov)
To study the cellular mechanisms for this post-transcriptional control and to determine the contribution of mRNA stability to GAP-43 gene expression, we examined the effects of several agents that affect PC12 cell differentiation on the level of induction and rate of degradation of the GAP-43 mRNA. (rupress.org)
The phorbol ester-induced stabilization of GAP-43 mRNA was blocked by the protein kinase inhibitor polymyxin B and was partially inhibited by dexamethasone, an agent that blocks GAP-43 expression and neuronal differentiation in PC12 cells. (rupress.org)
PC12 pheochromocytoma cells that mimic central dopaminergic neurons and represent a model for neuronal differentiation were cultured on collagen-coated fibres to evaluate cell response dependence on substrate topography. (unipi.it)
Cell differentiation was examined in terms of neurite number, orientation and length 6 days after administration of nerve growth factor (NGF). (unipi.it)
These results show essential roles of R-Ras GAP in development and differentiation: its expression is needed for embryonic development of blood vessel barriers, whereas its down-regulation facilitates NGF-induced neurite formation of PC12 cells via maintaining activated R-Ras. (elsevierpure.com)
Here, we report that Gβγ, an important component of the GPCR pathway, is critical for NGF-induced neuronal differentiation of PC12 cells. (biomedcentral.com)
In further support of a role of Gβγ-MT interaction in neuronal differentiation, it was observed that overexpression of Gβγ in PC12 cells induced neurite outgrowth in the absence of added NGF. (biomedcentral.com)
Over the years, pheochromocytoma (PC12) cells have been used as a model to study neuronal differentiation because they respond to nerve growth factor (NGF) and exhibit a typical phenotype of neuronal cells sending out neurites [ 4 ]. (biomedcentral.com)
Studies in developing rodents indicate that nicotine is a neuroteratogen that disrupts brain development by stimulating nicotinic acetylcholine receptors (nAChRs) that control neural cell replication and differentiation. (nature.com)
Essentially, by providing excessive cholinergic stimulation throughout fetal life, nicotine discoordinates the numerous events in cell replication, differentiation and synaptic development that are necessary to the proper assembly of the mammalian brain. (nature.com)
Objective: To investigate the induction of tanshinone [1 A (Tan fl A) on the differentiation of human placenta-derived mesenchymal stem cells (hPDMSCs) into cardiomyocytes, and to provide an experimental basis for Tan IT A as a cardiomyocytc differentiation inducer. (researchgate.net)
Gain and loss of function studies have indicated that miRNAs play a critical role in the regulation of all key biological functions such as development, cell proliferation, cell differentiation, and apoptosis [ 3 , 4 ]. (hindawi.com)
Magnolol induces the distribvutional changes of p160 and adipose differentiation-related protein in adrenal cells. (ntu.edu.tw)
Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. (cshlpress.com)

Their embryological origin with neuroblastic cells means they can easily differentiate into neuron-like cells even though they are not considered adult neurons. (wikipedia.org)
These cells are popular because they are able to synthesize and release catecholamines in a similar manner as dopaminergic neurons and adrenal medullary chromaffin cells [ 4 ]. (biomedcentral.com)
We believe that they may play an important role as uncommitted cells (like stem cells) start converting into neurons. (ycp.edu)
To do this, we remove RNA molecules from cells before and after they turn into neurons and then look for circular RNAs whose levels change. (ycp.edu)

Cytotoxic assays have been shown that iHg (5 mu M) caused oxidative stress and intrinsic apoptosis via ROS generation, oxidizing glutathione, damaging DNA, degrading cell membrane integrity, down-regulating mTOR, p-mTOR, akt and ERK1, and up-regulating cleaved caspase 3 and cytochrome c release in PC12 cells 48 h after incubation. (hokudai.ac.jp)
Co-treatment of Se (5 mu M) inhibited intrinsic apoptosis and oxidative stress induced by iHg (5 mu M) via inhibiting ROS formation, boosting GPx contents, increasing reduced glutathione, limiting DNA degradation, improving cell membrane integrity, up-regulating mTOR, p-mTOR, akt, ERK1 and caspase 3, and down-regulating cleaved caspase 3 and cytochrome c leakage in PC12 cells. (hokudai.ac.jp)
Aims: Increasing evidence displays that deposition of aggregated β-amyloid (Aβ) leads to neuronal cell apoptosis, thus aggravates the pathological progression of Alzheimer's disease (AD). (elsevierpure.com)
Main methods: MTT, DCFH-DA assay, LDH release assay, Fluo-3 AM assay, Flow cytometry and Western blot were used to examine cell viability, ROS level, LDH release, intracellular Ca 2+ concentration, cell apoptosis and related proteins level respectively. (elsevierpure.com)
In addition, D3T pretreatment inhibited Aβ 1-42 induced cell apoptosis as well as protein levels of Bax and Caspase-3 in PC12 cells. (elsevierpure.com)
Significance: Taken together, these findings suggest that D3T protects PC12 cells against Aβ 1-42 induced apoptosis through activation of the ERK1/2 pathway. (elsevierpure.com)
Nerve growth factor (NGF) is known to produce nitric oxide (NO) in PC12 cells, and an iron-NO complex such as dinitro-iron complex (DNIC) is found to keep the cells from apoptosis by inhibiting their caspase activation. (en-journal.org)
Apoptosis allows for efficient and immunologically silent removal of damaged or superfluous cells in multicellular organisms. (nature.com)
the expression levels of protein associated with apoptosis and endoplasmic reticulum stress (ERS) of SKO-007 cells in various groups were detected by Western blotting method. (researchgate.net)
Objective: To investigate the effects of prion protein 106-126 peptide on inducing apoptosis in differentiated PC12 cells. (researchgate.net)
Conclusion: Prion protein 106-126 peptide can induce apoptosis in differentiated PC12 cells and present cellular toxicity definitely. (researchgate.net)
Our results showed that lip OB significantly ameliorated MPTP-induced motor deficits and dopaminergic neuron loss in vivo and prevented MPP + -triggered cell viability reduction and apoptosis in vitro. (hindawi.com)
Need for caspases in apoptosis of trophic factor-deprived PC12 cells. (offbeatguides.com)

We report here that expression of constitutively active ASK1 (ASK1DeltaN) induces neurite outgrowth in the rat pheochromocytoma cell line PC12. (nih.gov)

Key findings: In the present study, we found that D3T pretreatment significantly increased cell viability and decreased reactive oxygen species (ROS) levels, lactate dehydrogenase (LDH) levels and the intracellular calcium concentration of rat pheochromocytoma (PC12) cells after Aβ 1-42 exposure. (elsevierpure.com)
Deltamethrin exposure promoted free radical formation in rat brain and reactive oxygen species generation in PC12 cells. (cdc.gov)

Therefore, we examined whether any increase of intracellular iron in serum-starved PC12 cells could block the cell death even in the presence of NGF (N-death). (en-journal.org)
These observations are the first documented intracellular effects noted for the complement molecule C5a in in-vitro cultured cells. (edu.au)
Pretreatment of cells with pertussis toxin or intracellular infusion of the GDP analogue guanosine-5'-O-(2-thiodiphosphate) was without effects on the control Ca2+ channel current but abolished its hormonal inhibition. (mdc-berlin.de)
Autophagy has a variety of complex physiological and pathophysiological roles, such as adaptation to nutrient starvation, clearance of damaged intracellular proteins and organelles, cell development, antiaging, elimination of microorganisms, cell death, tumor suppression, and antigen presentation. (moleculardevices.com)

In addition to the monoamine (dopamine and norepinephrine) pathway, PC12 cells have been reported to express both the kynurenine and serotonin pathways. (wikipedia.org)
A contrary view regarding expression of the serotonin pathway by PC12 cells was expressed by the investigators who first established this cell line. (wikipedia.org)
Because the effects of local anesthetics on cellular signal transduction in neuronal cells are not well-known, the authors investigated whether they affect the MAP kinase signaling pathway using PC12 cells. (asahq.org)
We examined the specific signaling pathway that regulates the transcription of B/K in PC12 cells. (kyobobook.co.kr)
Neuroprotective effects of NDEELNK from sea cucumber ovum against scopolamine-induced PC12 cell damage through enhancing energy metabolism and upregulation of the PKA/BDNF/NGF signaling pathway. (bvsalud.org)
B-Raf-dependent regulation of the MEK-1/mitogen-activated protein kinase pathway in PC12 cells and regulation by cyclic AMP. (wikidata.org)
Thus, the three structurally related compounds, MPP + , paraquat, and analog 1, showed different effects on the mitochondrial respiratory chain and the glycolytic pathway in PC 12 cells. (elsevierpure.com)
Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway. (uib.no)

PC12 cells treated for 10-14 days with nerve growth factor had no release of vesicles from the cell body which indicates the aggregation of vesicles in the ends of the neurites. (wikipedia.org)
Differentiated PC12 cells were characterized by highly aligned and longer neurites on parallel PHB fibres with respect to random fibres, thereby demonstrating the suitability of parallel PHB fibres for further studies in peripheral nervous system regeneration. (unipi.it)

We also observed that ASK1DeltaN induced expression of several neuron-specific proteins and phosphorylation of neurofilament proteins, confirming that PC12 cells differentiated into mature neuronal cells by ASK1. (nih.gov)
Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicant-induced cell death. (nih.gov)

We found that R-Ras GAP is down-regulated during neurite formation in rat pheochromocytoma PC12 cells by nerve growth factor (NGF), which is blocked by the transient expression of R-Ras gap or dominant negative R-ras cDNA. (elsevierpure.com)

Evidence suggests that nerve growth factor (NGF) induces neurite outgrowth from PC12 cells by activating the receptor tyrosine kinase, TrkA. (biomedcentral.com)

PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor or dexamethasone. (wikipedia.org)
Treatment of PC12 cells with nerve growth factor creates cells with long processes known as neurite varicosities, which contain small amounts of vesicles. (wikipedia.org)
We have previously shown that nerve growth factor (NGF) selectively stabilizes the GAP-43 mRNA in PC12 cells. (rupress.org)
The exact function of this protein is not known, but it plays an important role in nerve cells homeostasis. (ximbio.com)
Our results demonstrated that NDEELNK supplementation alleviated scopolamine -induced PC12 cell damage by improving the cholinergic system, increasing energy metabolism and upregulating the expression of phosphorylated protein kinase A (p-PKA), brain-derived neurotrophic factor (BNDF) and nerve growth factor ( NGF ) signaling proteins in in vitro experiments. (bvsalud.org)
Methods: After differentiated by nerve growth factor (NGF), the PC12 cells were infected by prion protein 106-126 peptide. (researchgate.net)
the chromaffin cells settle near the sympathetic ganglia, the vagus nerve, paraganglia, and carotid arteries. (medscape.com)

The vesicles hold catecholamines, mostly dopamine, but also limited amount of norepinephrine, and release of these neurotransmitters give rise to spikes due to changes in current similar to chromaffin cells. (wikipedia.org)
PC12 cells have been researched to evaluate the potential for pharmaceutical alternation of dopamine metabolites such as DOPAL, an autotoxin implicated in the pathology of Parkinson's disease. (wikipedia.org)
Cell adhesion, viability and proliferation, as well as dopamine production were evaluated after three days since seeding. (unipi.it)
Results showed that proliferating PC12 cells secreted a higher quantity of dopamine on fibres with respect to control cultures and as a result, a possible use of PHB fibres was considered for cell transplantation in the central nervous system when local production of dopamine is impaired. (unipi.it)
Effects of polychlorinated biphenyls on dopamine release from PC12 cells. (cdc.gov)

PC12 cell line use has given much information to the function of proteins underlying vesicle fusion. (wikipedia.org)
Four pertussis toxin-sensitive G proteins were identified in membranes of PC-12 cells: two members of the Gi family, Gi1 and Gi2, and two members of the Go family, Go2 and another Go subtype (possibly Go1). (mdc-berlin.de)
A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)

Together with previous reports indicating that ASK1 functions as a pro-apoptotic signaling intermediate, these results suggest that ASK1 has a broad range of biological activities depending on cell types and/or cellular context. (nih.gov)
Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking. (ntu.edu.tw)

The purpose of this research was to investigate ameliorations of Se counter to iHg-mediated toxicity in PC12 cells. (hokudai.ac.jp)
Both linoleic acid and arachidonic acid are able to inhibit MPP + -induced toxicity in PC12 cells. (biomedcentral.com)

Moreover, ASK1DeltaN-expressing PC12 cells survived in serum-starved condition. (nih.gov)
Previously we observed that serum-starved PC12 cells maintained their viability only for a couple of days even in the presence of NGF. (en-journal.org)

Cell viability was then assessed by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (biomedcentral.com)
Cells treated with 500 μM MPP + for a day reduced cell viability to ~70% as compared to control group. (biomedcentral.com)
Cell viability was determined by using the MTT assay and the morphological changes were observed. (researchgate.net)

In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

ASK1DeltaN-induced neurite outgrowth was strongly inhibited by treatment with the p38 inhibitor SB203580 but not with the MEK inhibitors, suggesting that activation of p38, rather than of ERK, is required for the neurite-inducing activity of ASK1 in PC12 cells. (nih.gov)
Neuronal outgrowth is a complex process in which two distinct domains emerge from the cell body: a long, thin axon that transmits signals, and multiple shorter dendrites, which are specialized primarily for receiving signals. (biomedcentral.com)
Similarly, indices of cell loss (reduced DNA), cell size and neuritic outgrowth (protein/DNA and membrane/total protein ratios) were distinct for each region and did not necessarily follow the rank order of nAChR upregulation, suggesting the involvement of additional mechanisms such as oxidative stress. (nature.com)

Possible employment of cell-specific peptide for the specific adsorption and uptake by cells. (msk.ru)
It was shown, that apoprotein E 139-158 peptide increases liposomal binding followed by receptor-mediated endocytosis by cells PC12. (msk.ru)
These results demonstrated that the sea cucumber ovum peptide -derived NDEELNK might play a protective role in PC12 cells . (bvsalud.org)

In the current study, the potential of two omega-6 fatty acids (i.e. arachidonic acid and linoleic acid) in alleviating 1-methyl-4-phenylpyridinium (MPP + )-induced cytotoxicity in PC12 cells was examined. (biomedcentral.com)

Using PC12 cells, we demonstrated that exposure to C5a led to inhibition of mitochondrial respiration, dehydrogenase and cytochrome c oxidase activities. (edu.au)
The untreated cells were stained with the orange mitochondrial integrity dye. (moleculardevices.com)

Posttranscriptional regulation of GAP-43 gene expression in PC12 cells through protein kinase C-dependent stabilization of the mRNA. (rupress.org)
By establishing a PC12 subclone that stably expresses exogenous R-Ras GAP, it was found that NGF reduced endogenous R-Ras GAP but not exogenous R-Ras GAP, suggesting that down-regulation of R-Ras GAP occurs at the transcription level. (elsevierpure.com)
In summary, our data suggest that the activation of AMPK and its regulation of cell surface GLUT3 expression is critical in mediating neuronal tolerance to excitotoxicity. (jneurosci.org)

PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla, that have an embryonic origin from the neural crest that has a mixture of neuroblastic cells and eosinophilic cells. (wikipedia.org)
A CELL LINE derived from a PHEOCHROMOCYTOMA of the rat ADRENAL MEDULLA . (online-medical-dictionary.org)
Biochemical studies have suggested a voltage-dependent dihydropyridine-sensitive catecholamine release in adrenal chromaffin cells. (mdc-berlin.de)
PC12, a cell line derived from a pheochromocytoma of the rat adrenal medulla, is used widely as a model for catecholaminergic transmission. (biomedcentral.com)

Overexpression of miR-339 downregulated Sirt2 expression, while knockdown of miR-339 upregulated Sirt2 expression in human SH-SY5Y cells and rat PC12 cells. (hindawi.com)
Overexpression of neuronal intermediate filament protein internexin in PC 12 cells. (ntu.edu.tw)

We examined effects of three structurally related pyridinium compounds, 1-methyl-4-phenylpyridinium (MPP + ), paraquat, and l-methyl-4-(4'- nitrophenyl) pyridinium (analog 1), on the energy metabolism in pheochromocytoma PC12 cells. (elsevierpure.com)

In addition to maintenance of membrane integrity, apoptotic cells can be discriminated from viable counterparts based on several morphological hallmarks, including cell contraction, nuclear condensation and fragmentation, and actomyosin contraction-dependent membrane blebbing and apoptotic body formation. (nature.com)

Materials and Methods: In this study, neuronal-like PC12 cells with were pretreated with different concentrations of gemfibrozil and H2O2, concomitantly. (phypha.ir)
Methods: The human multiple myeloma SKO-007 cells in logarithm growth phase were selected and randomly divided into control group, LDM group, BZM group, and BZM combined with LDM group. (researchgate.net)

Initially, the cells were pretreated with different concentrations of pulp hydroalcoholic extract (PHE), pulp aqueous extract (PAE) and pomegranate juice (PJ) for 2 h and then deprived of serum/glucose (SGD) for 6 and 12 h. (greenmedinfo.com)

GenJet DNA In Vitro Transfection Reagent for PC12 cell is pre-optimized for most efficiently trans fecting PC12 cells. (signagen.com)

Notably, inhibition of the CaMKK (calmodulin-dependent protein kinase kinase) had little affect on GLUT translocation, whereas the inhibition or knockdown of AMPK (compound C, siRNA) activity prevented GLUT3 translocation to the cell surface after glutamate excitation. (jneurosci.org)
We have characterized an AMP-mediated activation of AMPK that facilitates the translocation of GLUT3 to the neuronal cell surface after excitation. (jneurosci.org)

Cross-linking of p-selectin glycoprotein ligand-1 induced death of activated T cells. (ntu.edu.tw)

SIGNALS initiated by neurotransmitter release are transferred to the nuclei of the postsynaptic cells to activate specific programs of gene expression, thus influencing synaptic functions. (asahq.org)
Fetal brain regions and peripheral tissues were examined for nAChR subtypes, other neurotransmitter receptors, and indices of cell signaling and cell damage. (nature.com)

Chronic exposure of rats resulted in increased thyroid follicular cell tumors from sustained perturbation of thyroid hormone homeostasis. (cdc.gov)

The cells were stimulated with either 50 mM KCl or 1 microM ionomycin, and activated MAP kinase was thus immunoprecipitated. (asahq.org)
Pretreatment of the cells with 1 mM (ethylenedioxy)-diethyl-enedinitrilotetraacetic acid or 5 micron nifedipine blocked the MAP kinase activation induced by 50 mM KCl, whereas pretreatment with 2 microM omega-conotoxin GIVA did not. (asahq.org)
Dibucaine and tetracaine at clinical concentrations were found to inhibit the activation of MAP kinase and the expression of c-Fos mediated by L-type Ca2+ channels in PC12 cells. (asahq.org)
3,4 MAP kinase is highly expressed in the central nervous system, primarily in such postsynaptic structures as neuronal cell bodies and the bases of the proximal dendrites. (asahq.org)
Nuclear efflux of heterogeneous nuclear ribonucleoprotein C1/C2 in apoptotic cells: a novel nuclear export dependent on Rho-associated kinase activation. (ntu.edu.tw)

PC12 cells were purchased from the American Type Culture Collection (ATCC, CRL-1721.1). (biomedcentral.com)

This cell line has been used to understand the role of synaptotagmin in vesicle-cell membrane fusion. (wikipedia.org)

The PC-12 HD-Q23 cell lines 14 and 7 can be used for in-vitro assay development for neurodegenerative diseases. (ximbio.com)
The PC12 human neuroblastoma cell line was used as a model for assay development. (moleculardevices.com)
In human SH-SY5Y cells, the luciferase assay implied that Sirt2 was likely a target of miRNA-339. (hindawi.com)

J Cell Biol (1993) 120 (5): 1263-1270. (rupress.org)
Cell Biol. (ntu.edu.tw)

Mechanism of neurotrophic action of nobiletin in PC12D cells. (rochester.edu)

Therefore, this engineered protein adsorption approach allows for the facile preparation of tunable, quantifiable, and reproducible surfaces for in vitro studies of cell-ligand interactions and for potential application as coatings on neural implants. (frontiersin.org)
Of specific interest is the role that nanoscale cell-surface interactions play in the biocompatibility of neural implants. (frontiersin.org)
Grenier states, "Our research could indicate that a novel genetic material had an influence in the development of a cancerous neural cell. (ycp.edu)
In the fifth week of fetal development, neuroblastic cells migrate from the thoracic neural crest to form the sympathetic chains and preaortic ganglia. (medscape.com)

Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)

The biosynthesis and storage of catecholamines in chromaffin cell tumors may differ from the biosynthesis and storage in the normal medulla. (medscape.com)

10 9 apoptotic events occurring per day in human adult tissues, it is surprisingly difficult to histologically detect apoptotic cells due to the rapid recognition and clearance of apoptotic cells. (nature.com)
Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. (researchgate.net)

This cell line was first cultured by Greene and Tischler in 1976. (wikipedia.org)
Other organics have been studied using this cell line to understand their effects on PC12 cells These types of studies show that use of PC12 cell line can be a model for past and future neurotoxicological studies. (wikipedia.org)
The PC12 cell line has been used to get more information about diseases of the brain. (wikipedia.org)
Effect of forskolin, dibutyryl cAMP and CGS21680 was significantly reduced in PKA-deficient PC12 cell line (PC12-123.7). (kyobobook.co.kr)
No PrP TSE or infectivity has been detected in any exposed cell line under study so far. (cdc.gov)
A higly phagocytic cell line TO from Atlantic salmon is CD83 positive and M-CSFR negative, indicating a dendritic-like cell type. (uib.no)
An epithelial cell line initiated from a human bone metastasis of a grade IV prostatic ADENOCARCINOMA. (bvsalud.org)

Cell surface sensors on immune cells that ordinarily recognize pathogen-associated molecular patterns produced by infectious agents may also interact with DAMPs, leading to the activation of signaling pathways resulting in a nonpathogen-induced 'sterile' inflammatory response. (nature.com)

1999. Transactivation activity of human, zebrafish, and rainbow trout aryl hydrocarbon receptors expressed in COS-7 cells: Greater insight into species differences in toxic potency of polychlorinated dibenzo- p -dioxin, dibenzofuran, and biphenyl congeners. (cdc.gov)
The Apoptotic Process of Human Bladder Carcinoma T24 Cells Induced by Retinoid. (ntu.edu.tw)

Linoleic acid (50 and 100 μM) significantly reduced MPP + -induced cell death back to ~85-90% of the control value. (biomedcentral.com)

Therefore, in this study we investigated the possible protective effects of different extracts of pomegranate against SGD-induced PC12 cells injury. (greenmedinfo.com)
Similarly, Vitamin C supplementation had mixed effects, increasing nAChR responses while providing protection from cell damage in the caudate, the brain region most susceptible to oxidative stress. (nature.com)
To evaluate effects on autophagy, cells were treated with various concentrations of chloroquine or verapamil for 48 h. (moleculardevices.com)
Furthermore, silencing PINK1 compromised the beneficial effects of lip OB on MPP + -treated PC12 cells. (hindawi.com)

The berry fruits are also capable of modulating signaling pathways involved in inflammation, cell survival, neurotransmission and enhancing neuroplasticity. (lww.com)

We used undifferentiated PC-12 cells to study the effect of epinephrine and carbachol on transmembranous currents. (mdc-berlin.de)
This is the first study to examine the protective role of linoleic acid and arachidonic acid and their potential interaction in a Parkinson's disease model simulated by exposing PC12 cells to MPP + neurotoxin. (biomedcentral.com)
The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. (cdc.gov)
Through it all they have remained persistent and hope to help understand the role of these circular RNAs they study in the development of PC-12 cells, while also learning more about the process of working in a laboratory. (ycp.edu)
In this study, we investigated the therapeutic effect of OB-loaded liposomes (lip OB) on a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine- (MPTP-) induced mouse model of PD and 1-methyl-4-phenylpyridinium- (MPP + -) induced cell model of PD. (hindawi.com)

In the last decade, the importance of topographic properties of extracellular environments has been shown to be essential to addressing cell response, especially when replacing damaged tissues with functional constructs obtained in vitro. (unipi.it)
Autophagy: renovation of cells and tissues. (moleculardevices.com)
The ideal hydrogels should be capable of promoting the development of new tissues and simulating the characteristics of target tissues, with the properties matching the cell-matrix constituents of autologous tissues. (hindawi.com)

As confirmation that ligand density in these engineered systems impacts neuronal cell behavior, we demonstrate that increasing the density of fibronectin-derived RGD ligands on coated surfaces while maintaining uniform protein surface coverage results in enhanced neurite extension of PC-12 cells. (frontiersin.org)

The algorithms detect and characterize small objects, such as autophagosomes or mitochondria in the cytoplasm of cells, while using the nuclear marker to segment cells. (moleculardevices.com)

in vitro evaluation of chemical-induced neurotoxicity in #Cells @ECACC cells in research! (altmetric.com)

All these results suggest that iron block the N-death by reducing the NO accumulated in a cell under a continual presence of NGF. (en-journal.org)

Anatomy2

Diseases3

Chemicals and Drugs8

Caused neuronal1

Differentiation18

Neurons4

Apoptosis13

Pheochromocytoma Cell Line1

Reactive oxygen2

Intracellular4

Pathway8

Neurites2

Neuronal cells2

Neurite formation1

Induces neurite outgrowth1

Nerve7

Dopamine5

Proteins4

Cellular3

Toxicity2

Serum-starved2

Viability3

Proliferation1

Outgrowth3

Peptide3

Cytotoxicity1

Mitochondrial2

Regulation3

Adrenal4

Overexpression2

Energy metabolism1

Morphological1

Methods2

Pretreated with different concentrations1

Reagent1

Translocation2

Glycoprotein1

Neurotransmitter2

Rats1

Kinase5

ATCC1

Membrane1

Assay3

Biol2

Neurotrophic1

Neural4

Monoclonal1

Chromaffin cell1

Apoptotic cells2

Line7

Molecular1

Human2

Significantly reduced1

Effects4

Survival1

Study5

Tissues3

Ligand1

Mitochondria1

Vitro1

Results1