• membrane
  • In neuroendocrine PC12 cells, immature secretory granules (ISGs) mature through homotypic fusion and membrane remodeling. (rupress.org)
  • EM autoradiography showed that 125I-NGF was localized at the plasma membrane and cytoplasmic compartments but did not accumulate in the nuclear chromatin or in the nuclear membrane compartment of cells analyzed after 1 hr and 1, 2, and 8 d of incubation with 125I-NGF. (jneurosci.org)
  • Without a basement membrane cells have nowhere to attach and all dependent activities such as cell migration and epithelial formation can no longer occur. (wikipedia.org)
  • Once PI3K is activated, it will localize AKT that is in the cytoplasm to the cell membrane where AKT is then phosphorylated to promote cell survival. (wikipedia.org)
  • It also hyperpolarizes the cell membrane through influencing the sodium-potassium pump and thereby rendering it less responsive to adrenergic stimulation. (wikipedia.org)
  • apoptosis
  • Similarly, with the annexin V apoptosis test, preconditioning protected the cell and this was enhanced by OA. (hindawi.com)
  • Pretreatment with FM (5, 10 μM) prior to H(2)O(2) exposure significantly elevated cell viability, reduced cell apoptosis by MTT assay, LDH assay, Hoechst 33258 dye staining, and FM also decreased the accumulation of reactive oxygen species (ROS) by DCFH-DA staining and NBT test. (biomedsearch.com)
  • For example, the PI3K/AKT pathway is used by laminin-111 (promotes cell-survival), 511 (prevents apoptosis with laminin 521), and 521 (stabilizes pluripotency of human embryonic stem cells). (wikipedia.org)
  • subcellular
  • 125I-NGF also was not detected in nuclear subcellular fractions prepared from cells grown in serum-supplemented medium either in suspension for 1 d or in monolayer cultures for 1 to 8 d. (jneurosci.org)
  • cytotoxicity
  • Protective effects of Gastrodia elata Blume on MPP+-induced cytotoxicity in human dopaminergic SH-SY5Y cells," Journal of Ethnopharmacology , vol. 130, no. 2, pp. 290-298, 2010. (hindawi.com)
  • protective
  • In this study, we investigated whether exposure to oleanolic acid (OA) enhances the protective effects of preconditioning on PC12 cells exposed to 6-hydroxydopamine (6-OHDA). (hindawi.com)
  • In preconditioned cells, we found that cell viability remained high following exposure to 6-OHDA (150 μ M). OA treatment enhanced the protective effects of preconditioning. (hindawi.com)
  • Protective effect of rAAV/ABAD-DP on H2O2-mediated changes in morphology of PC12 cells. (eurekalert.org)
  • Protective effects of bone morphogenetic protein 7 against amyloid-beta induced neurotoxicity in PC12 cells," Neuroscience , vol. 184, pp. 151-163, 2011. (hindawi.com)
  • J.-H. Jang and Y.-J. Surh, "Protective effect of resveratrol on β -amyloid-induced oxidative PC12 cell death," Free Radical Biology and Medicine , vol. 34, no. 8, pp. 1100-1110, 2003. (hindawi.com)
  • S.-Y. Hong, W.-S. Jeong, and M. Jun, "Protective effects of the key compounds isolated from Corni fructus against β -amyloid-induced neurotoxicity in pc12 cells," Molecules , vol. 17, no. 9, pp. 10831-10845, 2012. (hindawi.com)
  • Protective effects of Ginkgo biloba extract (EGb761) and its constituents quercetin and ginkgolide B against β -amyloid peptide-induced toxicity in SH-SY5Y cells," Chemico-Biological Interactions , vol. 181, no. 1, pp. 115-123, 2009. (hindawi.com)
  • It can have a protective antioxidant effect on cultured PC12 cells. (wikipedia.org)
  • biochemical
  • Gaseous signaling molecules are gaseous molecules that are either synthesised internally (endogenously) in the organism, tissue or cell or are received by the organism, tissue or cell from outside (say, from the atmosphere or hydrosphere, as in the case of oxygen) and that are used to transmit chemical signals which induce certain physiological or biochemical changes in the organism, tissue or cell. (wikipedia.org)
  • Kinase
  • We previously showed, using PC12nnr5 cells transfected with myc-tagged 14-3-3β and η) isoforms, that 14-3-3s form a complex with tyrosine hydroxylase (TH) particularly when the hydroxylase is phosphorylated at Ser-19 by endogeneous Ca 2+ , calmodulin-dependent protein kinase II 1 . (springer.com)
  • These signalling particles may have a role in the temporal and spatial regulation of kinase activity inside cells. (biochemj.org)
  • neuronal cells
  • Smilagenin attenuates beta amyloid (25-35)-induced degeneration of neuronal cells via stimulating the gene expression of brain-derived neurotrophic factor," Neuroscience , vol. 210, pp. 275-285, 2012. (hindawi.com)
  • effects
  • Other organics have been studied using this cell line to understand their effects on PC12 cells These types of studies show that use of PC12 cell line can be a model for past and future neurotoxicological studies. (wikipedia.org)
  • Anti-inflammatory effects of rhynchophylline and isorhynchophylline in mouse N9 microglial cells and the molecular mechanism," International Immunopharmacology , vol. 9, no. 13-14, pp. 1549-1554, 2009. (hindawi.com)
  • partly
  • PC12 was used partly for some forms of Parkinson's disease with finds that support interference after vesicle docking and prior to vesicle release. (wikipedia.org)
  • apoptotic
  • Cell viability and apoptotic ratio were assessed using the MTT and Annexin V staining tests, respectively. (hindawi.com)
  • attenuates
  • K. W. Zeng, H. Ko, H. O. Yang, and X. M. Wang, "Icariin attenuates β -amyloid-induced neurotoxicity by inhibition of tau protein hyperphosphorylation in PC12 cells," Neuropharmacology , vol. 59, no. 6, pp. 542-550, 2010. (hindawi.com)
  • PI3K
  • The pathway begins with the adhesion of the cell to the ECM for activation of the lipid-associated PI3K. (wikipedia.org)
  • adhesion
  • SH2B adaptor protein family members (SH2B1-3) regulate various physiological responses through affecting signaling, gene expression, and cell adhesion. (sigmaaldrich.com)
  • nuclei
  • Autoradiography of the nuclear pellet from these cells showed the grains to be located mainly over damaged nuclei or over cell debris between nuclei. (jneurosci.org)
  • nucleus
  • The dynamic reciprocity theory states that a cell's fate depends on the exchange of chemical signals between the extracellular matrix and the nucleus of the cell. (wikipedia.org)
  • mechanism
  • D. J. Selkoe, "Cell biology of the amyloid β -protein precursor and the mechanism of Alzheimer's disease," Annual Review of Cell Biology , vol. 10, pp. 373-403, 1994. (hindawi.com)
  • Treatment
  • NGF treatment increased the activities of long chain acyl-CoA synthetases (LCASs), including oleoyl-CoA synthetase and arachidonoyl-CoA synthetase, by 150-580% over control, but cholinephosphotransferase activity rose by only 60%, suggesting that the synthesis of DAG in the cells was increased to a greater extent than its utilization. (biomedsearch.com)
  • Surface
  • The linkage between cells and the ECM is formed by binding cell surface receptors to one end of the laminin α chain and binding ECM components to another region of the laminin. (wikipedia.org)
  • When α chains of laminin-111 bind to cell surface receptors integrins α1β1, α3β1, α4β1, α6β1 and Cdc42 GTPase are activated. (wikipedia.org)
  • In addition to modifying the surface, biomaterials can be modified in bulk, meaning that the cell signaling peptides and recognition sites are present not just on the surface but also throughout the bulk of the material. (wikipedia.org)
  • line
  • This cell line was first cultured by Greene and Tischler in 1976. (wikipedia.org)
  • Antibiotic & Antimycotic The PC12 cell line has been used to get more information about diseases of the brain. (wikipedia.org)
  • Another problem might be your PC12 cell line- if they've been carried too long and allowed to overgrow before splitting, then you start selecting for non- responsive cells because they grow faster than the ones that respond to NGF. (bio.net)
  • cultures
  • PC12 cells were grown as monolayer cultures in medium supplemented with serum in the presence of 125I-NGF. (jneurosci.org)
  • Interference with the nucleolin - RSV fusion protein interaction has been shown to be therapeutic against RSV infection in cell cultures and animal models. (wikipedia.org)
  • extracellular matrix
  • The injection of laminin-111, however, helps with Duchenne muscular dystrophy, a neuromuscular disease in which the connection between the extracellular matrix and cell cytoskeleton is lost. (wikipedia.org)