As the major cell precursors in osteogenesis, mesenchymal stem cells (MSCs) are indispensable for bone homeostasis and development. (nature.com)
This study aimed to construct polycaprolactone/nano-hydroxyapatite (PCL/nHA) composite scaffolds with different pore sizes and investigate the osteogenesis and histocompatibility of cortical bone mesenchymal stem cells (BMSCs-C) seeded on it after inoculation. (annalsoftransplantation.com)
Angiogenesis2
Biological functional annotations of the transcriptomes suggest that osteoblast precursors induce angiogenesis coupled with osteogenesis, and chondrocyte precursors have the potential to differentiate into myocytes. (ijbs.com)
The sWAT-MSC secretome contains many proteins involved in tissue improvement and differentiation, suchas variables participating in chondrogenesis, osteogenesis, and angiogenesis. (5htreceptor.com)
Differentiation3
Using a defined in vitro model of early chondrogenesis, we attempted to enrich for cells with an enhanced ability for chondrogenic differentiation by pre-exposure of mouse adipose-derived adult stromal cells (ADASs) to a hypoxic (2% oxygen) environment. (ox.ac.uk)
ADASs expanded in 2% oxygen tension exhibited enhanced early chondrogenic differentiation and diminished osteogenesis, suggesting that the reduced oxygen environment may favor chondroprogenitors. (ox.ac.uk)
Furthermore, re-oxygenation of the 2% oxygen-expanded ADASs before differentiation did not significantly affect early chondrogenesis. (ox.ac.uk)
The subsequent synergistic regulation of POL II carboxyterminal domain (CTD) phosphorylation by BRD4 and RPAP2 initiated ZBTB16 transcriptional elongation, which facilitated MSC osteogenesis via the key osteogenic transcription factor SP7. (nature.com)
Without SEs located on osteogenic genes, BRD4 is not able to bind to osteogenic identity genes due to its closed structure before osteogenesis. (nature.com)
During osteogenesis, histones on osteogenic identity genes are acetylated, and OB-gain SEs appear, enabling the binding of BRD4 to the osteogenic identity gene ZBTB16. (nature.com)
To study direct osteogenesis in hPDCs, cells were cultured as monolayers and stimulated with osteogenic media. (bmj.com)
Chapter 5 utilizes a series of CG scaffolds that were inspired by elements of distinct components of orthopedic interfaces (cartilage, tendon/ligament, and bone) to examine the role of biomaterial physical properties (relative density, mineral content) on biasing MSC phenotype in the presence of mixed soluble signals to drive osteogenesis or chondrogenesis. (illinois.edu)
Under this paradigm, the MCC and its underlying bone are thought to result from 2 closely linked but separate processes: chondrogenesis and osteogenesis. (tamu.edu)
Cranial2
Several differences in molecular mechanisms operating during chondrogenesis and skeletogenesis have been demonstrated in cranial bones originating from these different cell lineages. (cuni.cz)
Causes that have been discussed have included congenital predisposition, intrauterine pressure abnormalities, shortening of the central tendon of the diaphragm, thickened substernal ligament, congenital deficiency of the musculature in the cranial diaphragm, abnormal osteogenesis and chondrogenesis, upper respiratory obstruction, environmental factors, and posturing difficulties. (thecatclinic.com.au)
According to the experimental results of immunofluorescence staining, GAG content, and qPCR, the largest pore size, which is 200 μm in diameter, shows the best chondrogenesis result. (tmu.edu.tw)
Cells1
In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. (spandidos-publications.com)
Inflammation1
This study demonstrates that HLA-B27 does not play a direct role in chondrogenesis and osteogenesis but possibly rather an indirect role by influencing inflammation. (bmj.com)
Markers2
Results There was no difference in chondrogenesis markers or in colorimetric tests between HLA-B27 + and HLA-B7 + micromasses. (bmj.com)
Expression of osteogenesis markers and Alizarin red staining was comparable in the HLA-B27 + and the HLA-B7 + hPDCs in monolayers. (bmj.com)
Mechanisms1
To overcome these obstacles in the translation of novel findings to clinical applications, researchers must address the most common targets and upstream mechanisms mediating the key steps of MSC osteogenesis. (nature.com)
Expression2
Therefore, our study shows that SEs orchestrate the osteogenesis of MSCs by targeting ZBTB16 expression, which provides an attractive focus and therapeutic target for osteoporosis. (nature.com)
Chondrogenesis ( COL2 , ACAN , COL10 ) and osteogenesis ( OSC , ALP , RUNX2 ) marker expression was studied by quantitative RT-PCR. (bmj.com)