Oligonucleotide sequence analysis dna. Medical search. Frequent questions

They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes. (wikipedia.org)
In-solution capture utilizes pools of oligonucleotides or probes bound to magnetic beads, whose sequence has been designed to hybridize to exon regions. (digitaljournal.com)
After binding to genomic DNA, these probes are pulled down and washed, allowing exon regions to be selectively sequenced. (digitaljournal.com)
A clever solution to this problem is to modify oligonucleotide probes to equalize (normalize) the thermal stability of G:C and A:T base pairs formed upon hybridization to the target, thereby making hybridization dependent only on oligo length and not on base composition. (genelink.com)
To validate diagnostic ability, probes on the DNA microarray were hybridized with genomic DNA extracted from L. monocytogenes, other Listeria spp. (korea.ac.kr)
The cross-linked sample DNA was hybridized with sequence-specific oligonucleotide probes to identify HLA-B27 allele sequences. (cdc.gov)
For the hybridization assays, the region of the target gene associated with resistance is PCR amplified, and the labeled PCR products hybridized to oligonucleotide probes immobilized on a nitrocellulose strip or in a microarray. (cdc.gov)

Polymerase Chain Reaction Combined with Restriction Enzyme Analysis for Detection and Differentiation of Fowl Adenoviruses. (vin.com)
CONCLUSION: The DNA methylation signature of NSCLC affects the outcome of stage I patients, and it can be practically determined by user-friendly polymerase chain reaction assays. (edgehill.ac.uk)
HLA-B27 testing was performed on these samples using a polymerase chain reaction (PCR) to specifically replicate the DNA sequences encoding the HLA loci of interest. (cdc.gov)

The genotyping technique that has shown the highest discriminatory power so far is based on the use of outward-directed primers specific for the insertion sequence (IS) IS 2404 , in combination with an oligonucleotide that targets a repeated GC-rich motif ( 16 ). (cdc.gov)
Oligonucleotides are most widely used as PCR primers. (researchandmarkets.com)
This can be attributed to the large revenue share of oligonucleotide synthesis for production of PCR primers. (researchandmarkets.com)
PCR using viral DNA and primers from conserved regions of the fowl adenoviral hexon gene 3 produced an approximately 900 base pair fragment and preliminary analysis of the amplified DNA sequence suggests a novel adenovirus. (vin.com)
A PCR assay using 553V-specific oligonucleotide primers designed to screen for viruses in field samples is under development. (vin.com)
1985. The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis . (wisc.edu)
Exons 2 and 3 of the HLA-B locus were amplified with locus specific primers and the amplified DNA was arrayed onto seven replicate nylon membranes and immobilized by UV cross-linking. (cdc.gov)

N4-Ethyl-deoxycytidine (N4-Et-dC) is typically used to minimize the deleterious effect of large variations in GC content in target/probe sequences on the results produced by techniques involving simultaneous hybridization of many sequences, for example, DNA chip or reverse hybridization protocols (1). (genelink.com)
The DNA microarray showed 98-100% positive hybridization signals for the 16 strains of L. monocytogenes tested, 7-85% positive signals for 9 strains of other Listeria spp. (korea.ac.kr)
cDNA was prepared from the injured and control tibialis anterior (TA) muscle of mice 24 h postinjury and labeled with the fluorescent dye Cy5 or Cy3 prior to hybridization to a DNA microarray. (cdc.gov)

The patent provides the means for producing libraries of peptide structures for drug screening applications that are capable of folding or assuming their native conformations independently of artificial scaffolds or flanking sequences in the proteins from which they are derived. (genomeweb.com)
We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
6, 7 BRCA1 interacts with a variety of proteins and is involved in multiple cellular processes including DNA repair, transcription, and checkpoint control. (bmj.com)
11 Both proteins possess an N-terminal RING finger motif and two BRCA1 C-terminal (BRCT) domains present in numerous proteins involved in DNA repair and cell cycle regulation. (bmj.com)
BARD1, unlike BRCA1, also contains a centrally located sequence of three ankyrin repeats 11 that are found in many proteins involved in transcriptional regulation. (bmj.com)
DNA/RNA-binding repeats in PUR-alpha/beta/gamma and in hypothetical proteins from spirochetes and the Bacteroides-Cytophaga-Flexibacter bacteria. (embl-heidelberg.de)
As a result of studies conducted in our research group on chitin synthesis and considering the lack of information on chitinase ants, we propose, in this project, the production of different chimeric proteins (containing chitinase catalytic domain and modules for binding in chitin) and the study of its catalytic function against chitin and analysis of the products through HPLC and mass spectrometry. (fapesp.br)
The physical basis of model-free analysis of NMR relaxation data from proteins and complex fluids. (lu.se)

Gene ontology analysis based on these 349 CpGs (330 genes) suggested the involvement of a number of genes responsible for immune and inflammatory system pathways, responses to stress and external stimuli, as well as wound healing and coagulation cascades. (nih.gov)
Finally, we identified aberrant methylation in multiple genes also targeted by recurring DNA copy number alterations in ALL, suggesting that these genes are inactivated far more frequently than suggested by structural genomic analyses alone. (lu.se)
The recent advance in the microarray data analysis makes it easy to simultaneously measure the expression levels of several thousand genes. (hindawi.com)
Thus, extracting a small subset of genes containing valuable information about a given cancer is one of the principal challenges in the microarray data analysis [ 4 ]. (hindawi.com)
Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed genes (DEGs). (spandidos-publications.com)
Exome sequencing is a capture based method developed to identify variants in the coding region of genes which affect protein function. (digitaljournal.com)
Gene expression was studied by cDNA microarrray analyses using 4608 unique clones from liver-derived expressed sequence tag (EST) libraries fortified with clones of known liver genes representing ∼4000 genes. (houstonmethodist.org)
Examination of the expression of several early marker genes of preadipocyte differentiation through Northern blot analysis and of differentiation-dependent mitosis showed that TCDD did not interfere with the earliest known responses of preadipocytes to inducers of differentiation. (aspetjournals.org)
As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)
In the present study, oligonucleotide microarray analyses were used to examine the candidate genes that are involved in these early events of the muscle injury/repair process. (cdc.gov)
The microarray analysis, including 732 genes, was conducted in triplicate, and we describe only genes modulated by the injury showing a differential expression (both increased and decreased) 1.7-fold or greater (p (cdc.gov)
La determinación de un patrón de genes expresados al nivel de la TRANSCRIPCIÓN GENÉTICA bajo circunstancias específicas o en una célula específica. (bvsalud.org)

The method, initially developed for the solution-phase synthesis, was also implemented on low-cross-linked "popcorn" polystyrene,[26] and later on controlled pore glass (CPG, see "Solid support material" below), which initiated a massive research effort in solid-phase synthesis of oligonucleotides and eventually led to the automation of the oligonucleotide chain assembly. (3dresearchspecs.com)
More recently, high-throughput oligonucleotide synthesis where the solid support is contained in the wells of multi-well plates (most often, 96 or 384 wells per plate) became a method of choice for parallel synthesis of oligonucleotides on small scale. (3dresearchspecs.com)
Solid support material[edit] In contrast to organic solid-phase synthesis and peptide synthesis, the synthesis of oligonucleotides proceeds best on non-swellable or low-swellable solid supports. (3dresearchspecs.com)
Synthesis of oligonucleotides by the H-Phosphonate Method Thirty years later, this work inspired, independently, two research groups to adopt the H-phosphonate chemistry to the solid-phase synthesis using nucleoside H-phosphonate monoesters 7 as building blocks and pivaloyl chloride, 2,4,6-triisopropylbenzenesulfonyl chloride (TPS-Cl), and other compounds as activators. (3dresearchspecs.com)

Following this methodology, sets of tri- and tetradeoxyribonucleotides were synthesized and were enzymatically converted to longer oligonucleotides, which allowed elucidation of the genetic code. (wikipedia.org)
Previous studies of DNA methylation suggest epigenetic alterations may also be important, but an integrated genome-wide analysis of genetic and epigenetic alterations in ALL has not been performed. (lu.se)
We found that the different genetic subtypes of ALL are characterized by distinct DNA methylation signatures that exhibit significant. (lu.se)
For mycobacteria such as Mycobacterium tuberculosis and M. ulcerans , low intraspecies diversity limits the use of genetic fingerprinting techniques that are based on sequence diversity in selected genetic elements. (cdc.gov)
Genetic analyses suggest recent divergence of M. ulcerans from M. marinum , a well-known fish pathogen that can cause limited granulomatous skin infections in humans ( 14 ). (cdc.gov)
Standard molecular typing methods such as multilocus sequence typing, restriction fragment length polymorphism, and fingerprinting using variable number of tandem repeats have shown an apparent lack of genetic diversity of M. ulcerans within individual geographic regions, which is indicative of a clonal population structure. (cdc.gov)
Oligonucleotides are short sections of nucleic acids that are united utilizing enzyme chemistry and genetic engineering. (maximizemarketresearch.com)
Here we describe prime editing, a versatile and precise genome editing method that directly writes new genetic information into a specified DNA site using a catalytically impaired Cas9 endonuclease fused to an engineered reverse transcriptase, programmed with a prime editing guide RNA (pegRNA) that both specifies the target site and encodes the desired edit. (nature.com)
Single-cell genetic analysis of clonal dynamics in colorectal adenomas indicates CDX2 gain as a predictor of recurrence. (cancerindex.org)
Dr Sergey Yakushev, Head of Genetic Analysis at Microsynth, explained: "My department focuses on genetic analysis, using a range of methodologies - such as conventional PCR, sequencing, and DNA and RNA isolation - which require multiple liquid handling steps during sample preparation and analysis. (integra-biosciences.com)
In the middle of the 20th century, accumulating data suggested that DNA carries life's genetic information. (acs.org)
Market growth will be attributed to the availability of a range of products for wide applications, decreasing prices of DNA sequencing, increasing incidence of and awareness on genetic diseases and personalized medicine, increasing demand for pharmacogenomics in drug discovery and development, and the rising use of genetic analysis of animal and plant livestock. (emailwire.com)

For M. tuberculosis , M. bovis , and the various bacillus Calmette-Guérin daughter strains, genome-wide microarray analyses have identified large sequence polymorphisms ( 4 , 8 - 10 ). (cdc.gov)

The evolution of oligonucleotide synthesis saw four major methods of the formation of internucleosidic linkages and has been reviewed in the literature in great detail. (wikipedia.org)
In the early 1950s, Alexander Todd's group pioneered H-phosphonate and phosphate triester methods of oligonucleotide synthesis. (wikipedia.org)
Methods are claimed that enable the dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. (genomeweb.com)
The patent also claims methods for the enzymatic preparation of 5'-activated, 3'-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides. (genomeweb.com)
PATIENTS AND METHODS: A DNA methylation microarray that analyzes 450,000 CpG sites was used to study tumoral DNA obtained from 444 patients with NSCLC that included 237 stage I tumors. (edgehill.ac.uk)
All three statistical methods demonstrate AmpliSeq as a highly accurate method for differential gene expression analysis. (biomedcentral.com)
IS 6110 DNA fingerprinting was performed according to adopted by laboratories worldwide for studying the molecular standard methods (1,5). (cdc.gov)

While there are approximately 180,000 exons in the human genome, constituting less than 2% of total sequence, the exome contains ~80-90% of known disease causing variants making it a cost-effective alternative to whole genome sequencing. (digitaljournal.com)

After necessary washes, incubations and additions are performed, the solid phase is analyzed to detect a label indicative of binding of nucleic acid in the sample to the oligonucleotide on the transponder, such as fluorescence, color, radioactivity or the like. (justia.com)
1986. Fluorescence detection in automated DNA sequence analysis . (wisc.edu)

Data from whole transcriptome sequencing of 33 breast specimens in the Harbin Medical University Cancer Center cohort and The Cancer Genome Atlas was applied to identify and validate the landscape of tumor suppressor long noncoding RNAs, which was further validated by The Cancer Genome Atlas pancancer data including 33 cancer types and 12,839 patients. (cancerindex.org)
Whole transcriptome sequencing (RNA-seq) represents a powerful approach for whole transcriptome gene expression analysis. (biomedcentral.com)
Transcriptome analysis has enhanced our understanding of the molecular constituents of cells and tissues. (biomedcentral.com)
Whole-transcriptome sequencing (RNA-seq) technology has emerged as a revolutionary platform for genome-wide quantification of mRNA transcripts. (biomedcentral.com)

We analyzed 137 B-lineage and 30 T-lineage childhood ALL cases using microarray analysis of DNA copy number alterations and gene expression, and genome-wide cytosine methylation profiling using the HpaII tiny fragment enrichment by ligation-mediated PCR (HELP) assay. (lu.se)
A total of six pairs of cancerous and adjacent healthy tissue were obtained from patients with HNSCC and genome‑wide transcriptional sequencing was performed. (spandidos-publications.com)

However, the complete genome sequence of an organism is required for the design of synthetic oligonucleotide or PCR product-based microarrays. (cdc.gov)
Several technologies exist to evaluate these changes including microarrays and serial analysis of gene expression (Sage). (chemistryworld.com)
In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)

North America is expected to hold the largest market size for the oligonucleotide synthesis globally. (maximizemarketresearch.com)
The key regions covered in the Whole Exome Sequencing market report are North America, Europe, China, Japan, Southeast Asia, India and Central and South America, etc. (digitaljournal.com)

We used this method for the differential genomic analysis of M. ulcerans, a human pathogen for which the fully assembled and annotated genome sequence was not available at the time of the study. (cdc.gov)
With the development of next-generation sequencing (NGS), research has focused on comprehensive genomic analysis based on tumor molecular features, such as somatic mutations and copy number variation. (spandidos-publications.com)
This comparative genomic analysis highlights high chromosomal synteny across the species yet diverse plasmid composition. (cdc.gov)

Limited success in clinical trials for oligonucleotide therapeutics and gene therapy restricts the growth of antisense oligonucleotide. (researchandmarkets.com)

Total RNA is converted to double-stranded complementary DNA molecules, which are cleaved to form tags. (chemistryworld.com)

Loop Genomics has launched its LoopSeq Bacterial Genome Sequencing Kit and Service for de novo bacterial genome assembly using synthetic long reads on Illumina sequencers. (genomeweb.com)

Products are often isolated by high-performance liquid chromatography (HPLC) to obtain the desired oligonucleotides in high purity. (wikipedia.org)
Sequence composition was confirmed via enzymatic digestion and purity by subsequent HPLC analysis. (concordia.ca)

When choosing between exome and whole genome sequencing (WGS), consider that exome sequencing has the advantage that oligonucleotides are designed to particular genomic regions where typical coverage with WGS is not enough for SNP calling. (digitaljournal.com)

These findings have implications for epidemiological-based studies of DNA methylation and provide a framework for the interpretation of findings from current and future studies. (nih.gov)

We performed more than 175 edits in human cells, including targeted insertions, deletions, and all 12 types of point mutation, without requiring double-strand breaks or donor DNA templates. (nature.com)
Typically, PCR is used to amplify a target sequence followed by a second assay to determine if the sequence contains a mutation associated with resistance. (cdc.gov)

We demonstrate that this method can be applied to multi-modal protein and RNA analyses. (nature.com)
Immunoblot analysis of human kidney cortex demonstrated that hOAT1 is an 80- to 90-kilodalton heterogeneous protein modified by abundant N -glycosylation. (aspetjournals.org)
Facilities include those for protein-sequence analysis, peptide and oligonucleotide synthesis, access to the synchrotron at Lawrence Berkeley National Lab for X-ray crystallography, and NMR spectrometry. (berkeley.edu)
Pur-alpha is a highly conserved, sequence-specific DNA- and RNA-binding protein involved in diverse cellular and viral functions including transcription, replication, and cell growth. (embl-heidelberg.de)
Pur-alpha can influence viral interaction through functional associations, for example with the Tat protein and TAR RNA of HIV-1, and with large T-antigen and DNA regulatory regions of JC virus. (embl-heidelberg.de)
Association of human Pur alpha with the retinoblastoma protein, Rb,regulates binding to the single-stranded DNA Pur alpha recognitionelement. (embl-heidelberg.de)
The retinoblastoma protein, Rb, is detected in extracts of monkey CV-1cells complexed with Pur alpha, a sequence-specific single-strandedDNA-binding protein implicated in control of gene transcription and DNAreplication. (embl-heidelberg.de)
Inthe absence of DNA, glutathione S-transferase-Pur alpha binds to p56RB, anNH2-terminal-truncated Rb protein purified from Escherichia coli,containing the T-antigen binding domain, to form multimeric complexes. (embl-heidelberg.de)
Sequence of cDNA comprising the human pur gene and sequence-specificsingle-stranded-DNA-binding properties of the encoded protein. (embl-heidelberg.de)
We report here the cloning and sequencing of a cDNA encodinga protein with strong affinity for the PUR element. (embl-heidelberg.de)
Analysis of mRNA for three isoforms of the CCAAT/enhancer binding protein (C/EBP) revealed that TCDD (5 nM) selectively inhibited the induction of C/EBP alpha mRNA but did not block the induction of C/EBP beta and C/EBP delta in response to differentiation inducers. (aspetjournals.org)
Immunoblot analysis with specific antibodies to each C/EBP isoform demonstrated that the levels of C/EBP delta and C/EBP beta protein were rapidly induced (by day 1) and then abrogated by day 4 and 8, respectively, in solvent-treated (control) cells. (aspetjournals.org)
Analysis of protein dynamics simulations by a stochastic point process approach. (lu.se)

It offers high quality products and services for molecular biologists who require DNA/RNA oligonucleotides, robust sequencing - such as high throughput Sanger sequencing - and analysis solutions, as well as outsourcing of contract research projects. (integra-biosciences.com)
PCR and Sanger sequencing were used to confirm mutations in and screen other family members where they were available. (molvis.org)

RNA from A. sexdens will be used to obtain cDNA that will be used as a template molecule in PCR for DNA amplification. (fapesp.br)
A negative control was included in each amplification run as a quality control check for contamination of the reactions by specimens or amplified product DNA. (cdc.gov)

Oligonucleotides find a variety of applications in molecular biology and medicine. (wikipedia.org)
The epidemiology and mode of transmission of M. ulcerans disease are not fully understood, partly because no molecular typing method with sufficiently high resolution for microepidemiologic analyses is available. (cdc.gov)
It provides comprehensive market analysis, highlighting the escalating use of oligonucleotide synthesis in diverse fields ranging from human health management to molecular diagnostics and therapeutics. (researchandmarkets.com)
The increasing usage of oligonucleotide synthesis in several field such as human health management, molecular diagnostics, and therapeutics have boosted the demand for this product thereby contributing to the market's revenue growth. (researchandmarkets.com)
The cytopathic effect was suggestive of an adenovirus and molecular analyses were performed to further characterize the virus isolate 553V. (vin.com)
Because molecular beacons can use different fluorophores, real-time PCR assays can be designed in which different DNA fragments or mutations can be amplified and detected simultaneously in the same tube. (cdc.gov)

Enzo Life Sciences of Farmingdale, NY, has received US Patent No. 8,574,881, "Compositions useful for detection or quantification of desirable target molecules, novel dyes, composite dyes, and oligonucleotides or polynucleotides comprising such dyes. (genomeweb.com)

Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence). (wikipedia.org)
The index number may define the nucleotide sequence of the oligonucleotide deposited on the surface of the particle, the catalog number of a DNA fragment deposited on the particle, index numbers of chemical steps which were involved in the chemical synthesis of an oligonucleotide bound to the particle, or some other relevant characteristics of the deposited molecules. (justia.com)

Colocalisation of BARD1 with BRCA1 and RAD51 in response to DNA damage indicates a role in DNA repair, 19, 20 which is supported by the recent observation of BARD1 participation along with BRCA1 in homology directed repair of chromosome breaks. (bmj.com)
CONCLUSIONS: To truly understand the biological differences observed between species of TBRF spirochetes, complete chromosome and plasmid sequences are needed. (cdc.gov)

21 Furthermore, interaction between the BARD1/BRCA1 heterodimer and cleavage stimulation factor subunit 1 (CSTF1, also called CstF-50) represses the polyadenylation machinery, presumably to prevent inappropriate mRNA processing at sites of DNA repair. (bmj.com)
Pmage is a technique that takes the best of Sage and high throughput DNA sequencing to obtain a global distribution of mRNA in any cell or tissue for a cost of about $1500 (?757) compared to about $10 000 for a Sage analysis, explained Seidman. (chemistryworld.com)
Thus, AmpliSeq stands as a very sensitive and cost-effective approach for very large scale gene expression analysis and mRNA marker screening with high accuracy. (biomedcentral.com)
The number of sequencing reads that map to each transcript is used to infer the abundance of mRNA molecules. (biomedcentral.com)

Moreover, our results indicate that linear regression is a valid statistical methodology for DNA methylation studies, despite the fact that the data do not always satisfy the assumptions of this test. (nih.gov)
To assess the performance of this new methodology, we performed a comprehensive comparison of AmpliSeq with RNA-seq using two well-established next-generation sequencing platforms (Illumina HiSeq and Ion Torrent Proton). (biomedcentral.com)

Typically, synthetic oligonucleotides are single-stranded DNA or RNA molecules around 15-25 bases in length. (wikipedia.org)
Oligonucleotides are found to be more effective than conventional small molecules. (researchandmarkets.com)
This technique enables the sequencing of all RNA molecules in a high-throughput manner. (biomedcentral.com)

Microsynth, founded in 1989, is a leading European company in nucleic acid synthesis, analysis and sequencing. (integra-biosciences.com)
Microsynth is using INTEGRA's MINI 96 and VIAFLO 96 handheld electronic pipettes in every step of sample preparation, in workflows ranging from nucleic acid isolation to next generation sequencing (NGS) and different types of PCR. (integra-biosciences.com)
In an electronically-indexed multiplex assay of this invention, two or more classes of transponders, each encoded with a different index number and constructed to bind a different nucleic acid sequence, are incubated with the sample in a single vessel. (justia.com)

Finally, we performed power calculations for EPIC based DNA methylation studies, demonstrating that existing studies with data on ~ 1000 samples are adequately powered to detect small differences at the majority of sites. (nih.gov)

CCK-8 assay, flow cytometric analysis, western blotting, orthotopic GBM model as well as transwell assay were performed to investigate the biological role of CASP5. (cancerindex.org)
The prognostic DNA methylation markers were validated by a single-methylation pyrosequencing assay in an independent cohort of 143 patients with stage I NSCLC. (edgehill.ac.uk)

To obtain the desired oligonucleotide, the building blocks are sequentially coupled to the growing oligonucleotide chain in the order required by the sequence of the product (see Synthetic cycle below). (wikipedia.org)
The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. (wikipedia.org)
A phosphoramidite respective to the 3′-terminal nucleoside residue is coupled to the universal solid support in the first synthetic cycle of oligonucleotide chain assembly using the standard protocols. (3dresearchspecs.com)
Special solid supports are used for the attachment of desired functional or reporter groups at the 3'-terminus of synthetic oligonucleotides. (3dresearchspecs.com)

This report also emphasizes the preference towards oligonucleotides by pharmaceutical and biotechnology companies due to their cost-effectiveness and rational drug design. (researchandmarkets.com)

We developed a DNA microarray that contains random genomic DNA fragments of Listeria monocytogenes, validated its diagnostic abilities using cells grown in laboratory media and milk, and established enrichment conditions for detection of a low population of L. monocytogenes in milk. (korea.ac.kr)
In milk, the detection limit of the DNA microarray was approximately 8 log CFU/mL. (korea.ac.kr)
Detection of Tick-borne Bacteria from Whole Blood Using 16S Ribosomal RNA Gene PCR Followed by Next-Generation Sequencing. (cdc.gov)

After the completion of the coupling reaction, a small percentage of the solid support-bound 5′-OH groups (0.1 to 1%) remains unreacted and needs to be permanently blocked from further chain elongation to prevent the formation of oligonucleotides with an internal base deletion commonly referred to as (n-1) shortmers. (3dresearchspecs.com)
Solid supports[edit] In solid-phase synthesis, an oligonucleotide being assembled is covalently bound, via its 3′-terminal hydroxy group, to a solid support material and remains attached to it over the entire course of the chain assembly. (3dresearchspecs.com)
The activated phosphoramidite in 1.5 - 20-fold excess over the support-bound material is then brought in contact with the starting solid support (first coupling) or a support-bound oligonucleotide precursor (following couplings) whose 5′-hydroxy group reacts with the activated phosphoramidite moiety of the incoming nucleoside phosphoramidite to form a phosphite triester linkage. (3dresearchspecs.com)
30] Once a phosphoramidite has been coupled to the solid support-bound oligonucleotide and the phosphite moieties have been converted to the P(V) species, the presence of the phosphate protection is not mandatory for the successful conducting of further coupling reactions. (3dresearchspecs.com)

First, we quantify the multiple testing burden and propose a standard statistical significance threshold for identifying DNA methylation sites that are associated with an outcome. (nih.gov)

Many of the changes appear to have been mediated by insertion sequence (IS) elements IS 2404 and IS 2606 , which have high copy numbers. (cdc.gov)
High-throughput sequencing data have been deposited to the NCBI Sequence Read Archive database under accession PRJNA565979 . (nature.com)
Notably, these results were consistent with those obtained using high‑throughput sequencing. (spandidos-publications.com)
Conversely, high concentrations of p56RB prevent Pur alphabinding to DNA. (embl-heidelberg.de)
Due to the higher thermal stability of C:C base pairs, high-GC content sequences may contain mis-matches yet still stably hybridize to a probe or target (resulting in false positives), while low-GC content sequences may perfectly match to probe or target but the strands may dissociate upon washing (resulting in false negatives). (genelink.com)
Sequencing is the high growth market, owing to its widening application areas of DNA sequencing technologies, technological advancements, and the availability of high-speed sequencing instruments are expected to contribute to the growth of the DNA sequencing market in the coming years. (emailwire.com)
The absence of physical dividers between the sites occupied by individual oligonucleotides, a very limited space on the surface of the microarray (one oligonucleotide sequence occupies a square 25×25 μm)[107] and the requirement of high fidelity of oligonucleotide synthesis dictate the use of site-selective 5′-deprotection techniques. (3dresearchspecs.com)
Integrated DNA Technologies has launched its oPools Oligo Pools, ready-to-use pools of high-quality DNA oligonucleotides. (genomeweb.com)

In 2019, the global Whole Exome Sequencing market size was USD 219.5 million and it is expected to reach USD 471.1 million by the end of 2026, with a CAGR of 11.4% during 2021-2026. (digitaljournal.com)

The oligonucleotide positive control dots were required to be positive for each probe and to be clearly distinguishable from all negative controls. (cdc.gov)
The performance of the line-probe assays relative to culture-based DS tests was evaluated in meta-analyses (3-5). (cdc.gov)

There is significant human exposure to polycyclic aromatic hydrocarbons (PAHs), many of which are bioactivated by the cytochrome P450 (P450) 1A family of enzymes to metabolites that are capable of covalently binding to DNA, a critical step in the initation of carcinogenesis. (houstonmethodist.org)

A majority of sTBRF spirochete genomes' plasmid sequences are either missing or are deposited as unassembled sequences. (cdc.gov)
Consequently, our goal was to generate complete, plasmid-resolved genomes for a comparative analysis of sTBRF species of the Western Hemisphere. (cdc.gov)
RESULTS: Utilizing a Borrelia specific pipeline, genomes of sTBRF spirochetes from the Western Hemisphere were sequenced and assembled using a combination of short- and long-read sequencing technologies. (cdc.gov)

DNA methylation is an important regulator of gene transcription, and global and specific gene methylation marks may vary with cigarette smoke exposure. (nih.gov)

Recent technological advances have accelerated single-cell analyses, but workflows remain expensive and complex. (nature.com)
The heterogeneity of the samples, special buffer conditions and liquid properties in DNA and RNA isolation workflows are not always compatible with liquid handling platforms. (integra-biosciences.com)

Second, we establish whether linear regression, the chosen statistical tool for the majority of studies, is appropriate and whether it is biased by the underlying distribution of DNA methylation data. (nih.gov)
Next, we tested whether the assumptions of linear regression are violated by DNA methylation data and found that the majority of sites do not satisfy the assumption of normal residuals. (nih.gov)
In this work, we are interested in gene expression data dimension reduction for cancer classification, which is a common task in most microarray data analysis studies. (hindawi.com)
However, given the mass of data emerging from this analysis, and commonalities they reveal between various phenomena/disorders, achieving 100% certainty may not be that easy. (medscimonit.com)
The Global Whole Exome Sequencing market Report provides Detailed analysis on the market position of the Whole Exome Sequencing Top players with stastical data, SWOT analysis, PESTAL analysis, latest developments across the globe. (digitaljournal.com)
The San Jose, California-based firm said the kit is plug-and-play and can easily replace existing sample preparation kits for bacterial genome sequencing experiments, using Loop's software for data analysis. (genomeweb.com)
Typically when data are presented, the fluor- escent intensity from the tissue is pseudo-colored red and the intensity from the reference green, and the logarithmic ratio of background corrected red and green intensities for each gene (spot) is subject to analysis (see Fig. 1 ). (lu.se)
The analysis of NHANES laboratory data must be conducted using the key survey design variables and basic demographic variables. (cdc.gov)

However, by using a prototype plasmid-based microarray that covered 10% of the genome, we found multiple genomic DNA deletions among 30 M. ulcerans clinical isolates of diverse geographic origins. (cdc.gov)

The global market for oligonucleotide synthesis may also face a restriction in its growth due to the lack of available resources. (maximizemarketresearch.com)
Genomic DNA of L. monocytogenes strain ATCC 19111 was fractionated by agarose gel electrophoresis after being cleaved using several different pairs of restriction enzymes. (korea.ac.kr)

The phosphoramidites of synthesized dimers were incorporated into one helical turn and 'sticky-end' DNA duplexes via solid-phase synthesis employing a DNA synthesizer. (concordia.ca)

All HLA-B27 positive specimens and 2.5% of HLA-B27 negative samples were confirmed using a second, independent DNA preparation in order to identify any sample discrepancies. (cdc.gov)

Patient A had no identified risk factor for HIV infection and was infected with a strain of HIV closely related to that of her dentist as determined by viral DNA sequencing. (cdc.gov)

Needless to say this same analysis can be performed on various types of cancers, including samples stored for many years under the right conditions. (medscimonit.com)

Anatomy5

Organisms10

Diseases1

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Phenomena and Processes87

Disciplines and Occupations5

Anthropology, Education, Sociology and Social Phenomena1

Information Science6

Health Care9

Probes7

Polymerase Chain Re3

Primers7

Hybridization3

Proteins8

Genes12

Synthesis of oligonucleotides4

Genetic12

Microarray analyses1

Methods7

Human Genome1

Fluorescence2

Transcriptome4

Genome-wide2

Microarrays3

North America2

Genomic analysis3

Antisense1

Complementary DNA1

Genomics1

HPLC2

Whole genome1

Studies of DNA methylation1

Mutation2

Protein13

Sanger2

Amplification2

Molecular6

Life Sciences1

Chemical synthesis2

Chromosome2

MRNA4

Methodology2

Molecules3

Nucleic acid3

Based DNA methylation1

Assay2

Synthetic4

Biotechnology companies1

Detection3

Bound4

Methylation sites1

High8

20191

Probe2

Covalently1

Genomes3

Transcription1

Workflows2

Data8

Clinical1

Restriction2

Solid-phase syn1

Specimens1

Viral1

Various types of cancers1