• 18 , 19 We surmised that cyclic cMOs containing an NfsB-cleavable linker could be used with analogous lines to knock down gene function in a tissue-specific manner (Figure 1 a). (cdc.gov)
  • This reagent contains a biotin moiety linked to an alkyne group through a spacer arm containing a cleavable disulfide linker. (broadpharm.com)
  • The 0 Å cross-linker CuCl 2 yields the reversible formation of disulfide bridge(s) between Toc64 and the established Toc complex subunits in purified outer envelope membranes. (silverchair.com)
  • Aminooxy-amido-PEG9-propargyl is a non-cleavable 9-unit PEG ADC linker that can be used to synthesize antibody-conjugated drugs (ADCs). (bocsci.com)
  • Biotin-Peg2-Amine Biotin-Peg2-Maleimide Biotin-Peg3-Amine Biotin-Peg4-NHS Mono BOC Cystamine[permanent dead link], Anachem Hansen, J. B. (wikipedia.org)
  • PEG reagents react with amine present in biological macromolecules. (creativepegworks.com)
  • This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)
  • [2] , [3] Today, they are among the most widely used amine-reactive crosslinking agents in bioconjugation. (quantabiodesign.com)
  • SS-PEG-SS (PEG-bis-Succinimidyl Succinate) is a homobifunctional cross-linking PEG reagent that reacts with amine groups, and the coupling reaction between NHS and amines generates new stable amide bonds. (axispharm.com)
  • Multi-arm PEG-thiols can crosslink itself to form disulfide bonds, which could be reversed under proper reducing conditions. (creativepegworks.com)
  • Under reducing conditions (50 mM dithiothreitol, 10 mM 2-mercaptoethanol or 1% sodium borohydride), the disulfide bonds are cleaved, releasing the biotin tag and any avidin conjugate bound to it. (broadpharm.com)
  • Such nanoparticles typically consist of a drug-loaded core and a hydrophilic shell, introducing disulfide bonds into the carrier backbone, side chains, or crosslinkers. (cd-bioparticles.net)
  • Disulfide bond breakage can occur not only in the cytoplasm, but also in lysosomes, due to the presence of gamma-interferon-induced lysosomal mercaptan (GILT), which can reduce disulfide bonds. (cd-bioparticles.net)
  • 11 The 4,5-dimethoxy-2-nitrobenzyl (DMNB)-containing linkers used in our previous study allowed optical MO uncaging, but cyclic cMOs could accommodate enzyme-cleavable linkers as well. (cdc.gov)
  • Mono-BOC-cystamine is used as a crosslinker for the synthesis of cleavable photo-cross-linking reagent. (wikipedia.org)
  • Disulfide Biotin alkyne is an azide-activated cleavable biotin probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. (broadpharm.com)
  • The 1.6 Å X-ray structure of the homodimeric A. fumigatus protein reveals unique properties including N-linked glycosylation and a Ca 2+ -binding site whose occupancy regulates activity. (nature.com)
  • Furthermore, it can be cross-linked in intact chloroplasts to a high molecular weight complex containing both Toc and Tic subunits and a precursor protein. (silverchair.com)
  • Therefore, we summarize here the recent advances of hydrogels that dissolve on demand, covering both chemical cross-linking cases and physical cross-linking cases. (biomedcentral.com)
  • Disulfide bond can exist stably outside the cell, but when the nanoparticles enter into the tumor cells, they can exchange disulfide bond with the high concentration of glutathione (GSH) in the cytoplasm, resulting in the disulfide bond breaking and the destruction of the carrier structure, so as to achieve the purpose of rapid drug release. (cd-bioparticles.net)
  • In addition, each protomer is N-linked glycosylated at three asparagine residues and stabilized by two disulphide bridges. (nature.com)
  • Due to their secretory functions, differentiation capabilities, specific homing effects through chemotaxis, distinctive therapeutic potentials, and ex vivo expandability, cells have become an attractive reagent for advanced therapeutic strategies. (biomedcentral.com)
  • TCO agarose is a 6% crosslinked agarose resin that is activated with TCO functional groups for covalent immobilization of tetrazine-modified biomolecules utilizing extremely fast Inverse electron demand Diels-Alder (IEDDA) reaction. (clickchemistrytools.com)
  • With growing chain bound to an insoluble support, simple filtration is effective in removing excess reagents and soluble by-products. (pharmaceutical-networking.com)
  • Washing with appropriate solvents ensure the complete removal of cleavage agents after the deprotection step, along with excess reagents and by-products resulting from the coupling step. (pharmaceutical-networking.com)
  • The following links lead to our MAL-dPEG ® -NHS ester products. (quantabiodesign.com)
  • Storage: -20C Application: TRUEPEG is used to crosslink PVA and dextran polymers to generate stable hydrogel. (thomassci.com)
  • This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. (frontiersin.org)
  • PEG reagents react with carboxylic acid in the presence of coupling agents such as DCC (N,N'-dicyclohexylcarbodiimide) and EDIC (N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide, HCl salt). (creativepegworks.com)
  • Application: EncapGel(TM)-Cx Kit is a ready-to-use, chemically crosslinked hydrogel kit for the encapsulation and delivery of drugs, biomolecules, and cells. (thomassci.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • Mono-BOC-cystamine is used as a crosslinker for the synthesis of cleavable photo-cross-linking reagent. (wikipedia.org)
  • Dithio-bis-maleimidoethane) is a homobifunctional and cleavable crosslinker that is cell membrane permeable. (proteochem.com)
  • DTME Crosslinker has sulfhydryl-reactive maleimides at both ends of a cleavable, 11-atom (13.3 angstrom) spacer arm, and DTME cross links occur via these maleimides. (proteochem.com)
  • DTME Crosslinker must be dissolved in an organic solvent, such as DMSO or DMF , then added to an aqueous crosslinking reaction. (proteochem.com)
  • For example, this brain-homing peptide can cross the blood-brain barrier. (lifetein.com)
  • While Si-containing polymers can often be deconstructed using chemical triggers such as fluoride, acids, and bases, they are resistant to cleavage by mild reagents such as biological nucleophiles, thus limiting their end-of-life options and potential environmental degradability. (bvsalud.org)
  • We conjugate two sequences together to form a disulfide bond. (lifetein.com)
  • Assaying one library (up to 900,000 sequences in our experiments) requires one week and reagents costing about US$2,000, excluding the cost of DNA synthesis and sequencing. (nature.com)
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
  • DTME crosslinking reagent contains a reducible disulfide (thiol) bond in the spacer arm that make it a good choice for various applications. (proteochem.com)
  • Under the acidic environment, the disulfide bond can be cleaved. (lifetein.com)
  • These disulfide-based prodrugs are important for cancer therapy. (lifetein.com)
  • Unfortunately, these breakthrough discoveries in both regenerative medicine and cancer immunotherapy using cells as therapeutic reagents soon faced a common problem: the inability to control cellular functions to maximize the therapeutic benefits. (biomedcentral.com)