• This report summarizes trends in cephalosporin susceptibility among N. gonorrhoeae isolates in the United States during 2000--2010 using data from the Gonococcal Isolate Surveillance Project (GISP). (cdc.gov)
  • State and local public health departments should promote maintenance of laboratory capability to culture N. gonorrhoeae to allow testing of isolates for cephalosporin resistance. (cdc.gov)
  • During August--October 2009, five of 55 (9.1%) N. gonorrhoeae isolates obtained from men with symptomatic urethritis tested at San Diego County's main municipal sexually transmitted disease (STD) clinic had high azithromycin MICs: three with 8 µ g/mL and two with 16 µ g/mL. (cdc.gov)
  • GISP conducts susceptibility testing of urethral N. gonorrhoeae isolates obtained from men with symptomatic urethritis seeking care at 29 U.S. STD clinics, including San Diego County's main municipal STD clinic. (cdc.gov)
  • The five N. gonorrhoeae isolates with high MICs (9.1%) obtained from the five patients were among 55 N. gonorrhoeae isolates obtained from men with symptomatic urethritis tested during the 3-month period. (cdc.gov)
  • On October 17, 1997, the STD Program of the San Diego Department of Health was notified by the GISP laboratory that the N. gonorrhoeae isolates from patients 1 and 2 were resistant to ciprofloxacin and ofloxacin (minimum inhibitory concentration {MIC} 16 ug/mL for both antibiotics). (cdc.gov)
  • Each month, N. gonorrhoeae isolates are collected from up to the first 25 men with gonococcal urethritis attending each of the participating sexually transmitted disease (STD) clinics at 27 sites. (cdc.gov)
  • Isolates of N gonorrhoeae from male urethritis patients attending four urological clinics in Hyogo and Osaka prefectures in Japan were collected during 2002. (bmj.com)
  • As some C. trachomatis isolates are reported not to carry cryptic plasmid or have deletion(s) in it [7], "RealLine Chlamydia trachomatis/ Neisseria gonorrhoeae" assay kit detects two DNA fragments from gyrA gene and cryptic plasmid, specific to C. trachomatis species. (roboscreen.com)
  • N. gonorrhoeae isolates (n=193) obtained in the Mogilev (n=142), Minsk (n=36) and Vitebsk (n=15) regions of Belarus in 2010 (n=72), 2011 (n=6), 2012 (n=75) and 2013 (n=40) were analyzed in regards to AMR using the Etest method and for molecular epidemiology with N. gonorrhoeae multi-antigen sequence typing (NG-MAST). (biomedcentral.com)
  • isolated on selective media for N. gonorrhoeae, isolates of other Neisseria spp. (firebaseapp.com)
  • Methods: WGS was used to identify previously reported potential resistance determinants in 681 N. gonorrhoeae isolates, from England, the USA and Canada, with phenotypes for cefixime, penicillin, azithromycin, ciprofloxacin and tetracycline determined as part of national surveillance programmes. (ox.ac.uk)
  • Activity of faropenem tested against Neisseria gonorrhoeae isolates including fluoroquinolone-resistant strains. (jmilabs.com)
  • We sequenced the whole genomes of 21 N. gonorrhoeae isolates collected in 2013-2014 by ARSP. (who.int)
  • Despite the small number of isolates studied, they were genetically diverse, as shown by the sequence types, the N. gonorrhoeae multiantigen sequence typing types and the tree. (who.int)
  • This first genomic survey of N. gonorrhoeae isolates collected by ARSP will be used to contextualize prospective surveillance. (who.int)
  • N. gonorrhoeae is the causative agent of gonorrhea and N. meningitidis is one cause of bacterial meningitis. (wikipedia.org)
  • A single 2 g dose of azithromycin effectively treats genitourinary infections caused by susceptible Neisseria gonorrhoeae and has been used to treat uncomplicated gonorrhea in persons with cephalosporin allergy. (cdc.gov)
  • N gonorrhoeae causes the sexually transmitted disease gonorrhea, among other diseases. (medscape.com)
  • Gonorrhea is a common N gonorrhoeae infection that is transmitted almost exclusively by sexual contact or perinatally and primarily affects the mucous membranes of the urethra and cervix and, less frequently, those of the rectum, oropharynx, and conjunctivae. (medscape.com)
  • Neisseria gonorrhoeae causes the sexually transmitted disease gonorrhea exclusively in humans and uses multiple strategies to infect, including acquisition of host sialic acids that cap and mask lipooligosaccharide termini, while restricting complement activation. (datadryad.org)
  • It causes a sexually transmitted disease, gonorrhea, which remains a major global public health concern since many identified strains of N. gonorrhoeae are resistant to most available antibiotics [8]. (roboscreen.com)
  • Neisseria gonorrhoeae is the causative agent of gonorrhea, a sexually transmitted infection (STI) of major importance. (peerj.com)
  • Neisseria gonorrhoeae , a Gram-negative bacterium, causes gonorrhea, the most common bacterial sexually transmitted infection (STIs), causing more than 106 million cases per year globally ( World Health Organization (WHO), 2012 ). (peerj.com)
  • Mar 6, 2020 Gonorrhea is a common sexually transmitted infection caused by the gram- negative intracellular diplococcus Neisseria gonorrhoeae. (firebaseapp.com)
  • Neisseria gonorrhoeae , also known as N. gonorrhoeae to its friends, is a gram-negative oval bacterium that infects humans, causing a number of infections including gonorrhea . (osmosis.org)
  • The word Neisseria came from Neisser Albert, a German physician who discovered it, while gonorrhea is from the Greek words "gonos" which means 'seed', and "rhoe" which means 'flow', meaning 'flow of seed', an illustration referring to the penile purulent discharge, which was mistakenly thought to be semen in infected males. (osmosis.org)
  • Neisseria gonorrhoeae is a gram-negative diplococcus, non-spore-forming, both oxidase and catalase-positive bacteria, which is known to cause a sexually transmitted infection (STI) called gonorrhea . (osmosis.org)
  • Neisseria gonorrhoeae (Gc) is the causative agent of the sexually transmitted infection gonorrhea. (emerging-researchers.org)
  • Robert Kirkcaldy] Neisseria gonorrhoeae is a bacterium that causes gonorrhea and it can infect the cervix, urethra, throat, and rectum during sex. (cdc.gov)
  • Since the introduction of antibiotics in the first half of the 20th century, Neisseria gonorrhoeae has successively developed resistance to each antibiotic recommended for gonorrhea treatment. (cdc.gov)
  • Neisseria gonorrhoeae, also known as gonococcus (singular), or gonococci (plural), is a species of Gram-negative diplococci bacteria isolated by Albert Neisser in 1879. (wikipedia.org)
  • The bacterium Neisseria gonorrhoeae (gonococcus), was discovered in 1879 by the German physician Albert Neisser, after whom it is also named. (wikilectures.eu)
  • Sexually transmitted infections caused by Neisseria gonorrhoeae are a cause of pelvic inflammatory disease in women, which can lead to serious reproductive complications including tubal infertility, ectopic pregnancy, and chronic pelvic pain. (cdc.gov)
  • The Frequency of Discordant Gyrase A Genotypes Among Cases of Multiple Neisseria gonorrhoeae Infections at Different Anatomic Sites. (aidshealth.org)
  • Chlamydia trachomatis and Neisseria gonorrhoeae infections in the rectum and pharynx are important extragenital reservoirs of infection. (escholarship.org)
  • These analytical performance data demonstrate that the Abbott CT/NG RealTime assay is an accurate, sensitive, and specific assay in rectal and pharyngeal specimens, supporting the potential of the assay for detection of rectal and pharyngeal C. trachomatis and N. gonorrhoeae infections. (escholarship.org)
  • D. Culture The current preferred laboratory method for the diagnosis of N. gonorrhoeae infections is the isolation and identification of the agent. (firebaseapp.com)
  • Nucleic acid amplification testing (NAAT) is the recommended method for initial screening or testing for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infections. (publichealthontario.ca)
  • We used N. gonorrhoeae spiked urine samples and samples from gonorrhoea infections to determine optimal DNA extraction methods that maximize the amount of N. gonorrhoeae DNA sequenced whilst minimizing contaminating host DNA. (ox.ac.uk)
  • In simulated infections the Qiagen UCP Pathogen Mini kit provided the highest ratio N. gonorrhoeae to human DNA and the most consistent results. (ox.ac.uk)
  • Depletion of human DNA with saponin increased N. gonorrhoeae yields in simulated infections, but decreased yields in clinical samples. (ox.ac.uk)
  • In simulated infections if ≥10 4 CFU/ml of N. gonorrhoeae was present, sequencing of the large majority of the genome was frequently achieved. (ox.ac.uk)
  • We used N. gonorrhoeae spiked urine samples and samples from gonorrhoea infections to determine optimal DNA extraction methods that maximize the amount of N. gonorrhoeae DNA sequenced whilst minimizing contaminating host DNA.ResultsIn simulated infections the Qiagen UCP Pathogen Mini kit provided the highest ratio N. gonorrhoeae to human DNA and the most consistent results. (ox.ac.uk)
  • Fluoroquinolone-resistant strains of Neisseria gonorrhoeae have been identified frequently during the 1990s in the Far East (2). (cdc.gov)
  • My role in this research project consisted of attempting to create three strains of Neisseria gonorrhoeae (Gc) that constitutively express a single opa protein. (emerging-researchers.org)
  • Susceptibility of Neisseria gonorrhoeae to cefotaxime and ceftizoxime. (bmj.com)
  • Decreasing susceptibility of Neisseria gonorrhoeae to fluoroquinolones has been reported in several countries. (bmj.com)
  • We investigated whether there was an association between annual antibiotic prescribing rates in each county and the antibiotic susceptibility of Neisseria gonorrhoeae . (cdc.gov)
  • The culture grew N. gonorrhoeae and was sent for antimicrobial susceptibility testing as part of the national Gonococcal Isolate Surveillance Project (GISP). (cdc.gov)
  • The Gonococcal Isolate Surveillance Project (GISP) was established in 1986 as a sentinel surveillance system to monitor trends in antimicrobial susceptibilities of N. gonorrhoeae strains in the United States. (cdc.gov)
  • Herein, the prevalence and trends of gonococcal AMR and molecular epidemiological characteristics of N. gonorrhoeae strains from 2010 to 2013 in Belarus, are described. (biomedcentral.com)
  • Neisseria gonorrhoeae is a species of Gram-negative diplococci non-motile bacteria from the genus Neisseria . (roboscreen.com)
  • Neisseria gonorrhoeae is a species of gram negative diplococci, which are non encapsulated, non motile and kidney shaped bacteria. (howmed.net)
  • Se hela listan på catalog.hardydiagnostics.com Gram stain for Neisseria gonorrhoeae is a quick and inexpensive test that works by detecting Gram-negative diplococci (the gonorrhoea bacteria) under a microscope. (firebaseapp.com)
  • All of the gram-negative diplococci were confirmed to be N gonorrhoeae by sugar fermentation studies. (firebaseapp.com)
  • The N gonorrhoeae are the intracellular gram-negative diplococci within the cytoplasm of the polymorphonuclear (PMN) cell marked with the small arrow. (firebaseapp.com)
  • N. gonorrhoeae typically live in pairs called diplococci, stacked side to side, so the pair looks like a coffee bean. (osmosis.org)
  • Pili are attractive targets for vaccines and therapeutics because of the key role they play in bacterial virulence as well as their prominent cell surface exposure, as shown in this scanning electron micrograph of Neisseria gonorrhoeae diplococci. (medscape.com)
  • Neisseria gonorrhoeae is a major cause of pelvic inflammatory disease, ectopic pregnancy, and infertility, and it can facilitate human immunodeficiency virus (HIV) transmission ( 1 ). (cdc.gov)
  • This agar preparation facilitates the growth of Neisseria species while inhibiting the growth of contaminating bacteria and fungi. (wikipedia.org)
  • Some antimicrobials, like vancomycin and nystatin are usually added to the Thayer-Martin agar, to inhibit the possible growth of undesired bacteria or fungi, and maximize the growth of Neisseria species. (osmosis.org)
  • Antibiotic resistance in N. gonorrhoeae is a growing public health concern, especially given its propensity to develop resistance easily. (wikipedia.org)
  • criteria for cefixime and ceftriaxone resistance in N. gonorrhoeae have not been defined ( 6 ). (cdc.gov)
  • Over the past decade, strains of N gonorrhoeae have been reported to develop high levels of resistance against several antimicrobial agents previously used for treatment of gonorrhoea. (bmj.com)
  • Gonorrhoea and widely spread antimicrobial resistance (AMR) in its etiological agent Neisseria gonorrhoeae are major public health concerns worldwide. (biomedcentral.com)
  • During 2010-2013, the N. gonorrhoeae population in Belarus displayed high and relatively stable resistance levels to tetracycline, ciprofloxacin, and penicillin G, while the resistance to azithromycin declined. (biomedcentral.com)
  • N. gonorrhoeae has developed antimicrobial resistance (AMR) to all drugs previously recommended for treatment of gonorrhoea. (biomedcentral.com)
  • We collected draft genome sequence data and associated metadata data on 76 N. gonorrhoeae strains from around the globe and searched for known determinants of antibiotics resistance within the strains. (peerj.com)
  • Neisseria gonorrhoeae (NG) culture is recommended plus NAAT when suspecting antimicrobial resistance, test of cure, symptomatic patients, pelvic inflammatory disease (PID), pregnancy, and sexual abuse/sexual assault. (publichealthontario.ca)
  • Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. (who.int)
  • We report a duplex real-time PCR assay for the simultaneous screening of mutations involved in fluoroquinolone resistance within gyrA and parC quninolone resistance-determining regions (QRDRs) in Neisseria gonorrhoeae. (pasteur.fr)
  • Objectives: We investigate whether WGS and simultaneous analysis of multiple resistance determinants can be used to predict antimicrobial susceptibilities to the level of MICs in N. gonorrhoeae. (ox.ac.uk)
  • There is a need for same-day culture-free diagnostics that identify infection and detect antimicrobial resistance.MethodsWe investigated if Nanopore sequencing can detect sufficient N. gonorrhoeae DNA to reconstruct whole genomes directly from urine samples. (ox.ac.uk)
  • Rapid antibiotic susceptibility testing (AST) for Neisseria gonorrhoeae (Ng) is critically needed to counter widespread antibiotic resistance . (bvsalud.org)
  • Antimicrobial resistance in Neisseria gonorrhoeae: Global surveillance and a call for international collaborative action. (who.int)
  • We hear a lot about how use of antibiotics in the United States can cause resistance in other bacteria, but we don't know whether this is the case with Neisseria gonorrhoeae . (cdc.gov)
  • On its surface, N. gonorrhoeae bears hair-like pili, surface proteins with various functions, and sugars called lipooligosaccharides. (wikipedia.org)
  • Dynamic polymeric protein filaments called type IV pili allow N. gonorrhoeae to adhere to and move along surfaces. (wikipedia.org)
  • N. gonorrhoeae is able to pull 100,000 times its own weight, and the pili used to do so are amongst the strongest biological motors known to date, exerting one nanonewton. (wikipedia.org)
  • Neisseria gonorrhoeae with numerous pili extending from the cell surface. (medscape.com)
  • Pili - type Fig 4: Gram Stain of N gonorrhoeae. (firebaseapp.com)
  • Knowledge of local N gonorrhoeae susceptibilities to various antimicrobials is important for establishing a rational treatment strategy in each region. (bmj.com)
  • Susceptibilities of N gonorrhoeae should be monitored periodically by region. (bmj.com)
  • Ceftriaxone and spectinomycin demonstrated lower MICs and so are recommended for N gonorrhoeae . (bmj.com)
  • We investigated MICs of N gonorrhoeae isolated from men with urethritis in an urban area of Japan to establish a rational treatment strategy appropriate to the area. (bmj.com)
  • The presence of bacteria resembling Neisseria gonnorhoeae in a man is diagnostic of gonnorhoea, however in women it is more difficult to diagnose, having a mixture of bacterial organisms present. (firebaseapp.com)
  • abstract = "Neisseria gonorrhoeae lipooligosaccharide (LOS) undergoes antigenic variation at a high rate, and this variation can be monitored by changes in a strain's ability to bind LOS-specific monoclonal antibodies. (uky.edu)
  • abstract = "Current data suggest that Neisseria gonorrhoeae is able to suppress the protective immune response at different levels, such as B and T lymphocytes and antigen-presenting cells. (unab.cl)
  • N. gonorrhoeae can cause infection of the genitals, throat, and eyes. (wikipedia.org)
  • Neisseria gonorrhoeae culture is indicated in the diagnosis of N gonorrhoeae infection. (medscape.com)
  • Positive culture indicates active infection with N gonorrhoeae . (medscape.com)
  • Mucosal infection with Neisseria gonorrhoeae. (jci.org)
  • Intended for use as a screening test for evidence of Neisseria gonorrhoeae infection. (mplnet.com)
  • No evidence of N gonorrhoeae infection by culture, gram stain of a smear of the discharge, or antigen or nucleic acid detection. (firebaseapp.com)
  • N. gonorrhoeae infection occurs following mucosal inoculation during vaginal, anal, or oral sexual contact. (brainkart.com)
  • N. gonorrhoeae could also be detected from urine in cobas PCR Media tubes and from urethral swabs, and in the presence of simulated Chlamydia co-infection. (ox.ac.uk)
  • N. gonorrhoeae could also be detected from urine in cobas PCR Media tubes and from urethral swabs, and in the presence of simulated Chlamydia co-infection.ConclusionUsing Nanopore sequencing of urine samples from men with urethral gonorrhoea sufficient data can be obtained to reconstruct whole genomes in the majority of samples without the need for culture. (ox.ac.uk)
  • Neisseria species are fastidious, Gram-negative cocci that require nutrient supplementation to grow in laboratory cultures. (wikipedia.org)
  • Of the 11 species of Neisseria that colonize humans, only two are pathogens. (wikipedia.org)
  • The exact formulation has changed over the years but includes agents active against gram-positive bacteria (vancomycin), gram-negative bacteria (colistin, trimethoprim), and fungi (nystatin, anisomycin) at concentrations that do not inhibit N gonorrhoeae . (medscape.com)
  • Instead, like any other Gram-negative bacteria, N. gonorrhoeae stains pink with safranin dye. (osmosis.org)
  • Neisseria gonorrhoeae infects primarily the mucosal membranes. (howmed.net)
  • Background: Tracking the spread of antimicrobial-resistant Neisseria gonorrhoeae is a major priority for national surveillance programmes. (ox.ac.uk)
  • N. gonorrhoeae is a gram-negative bacterium, because its cell wall has a thin peptidoglycan layer and so it doesn't retain purple dye used during Gram staining. (osmosis.org)
  • It causes the sexually transmitted disease gonorrhoeae , known as gonorrhoea in Czech. (wikilectures.eu)
  • Gonorrhoea, etiological agent Neisseria gonorrhoeae , is a public health concern globally. (biomedcentral.com)
  • Neisseria gonorrhoeae causes an acute, inflammatory disease of the urogenital tract called gonorrhoea . (wikilectures.eu)
  • In ten urine samples from men with symptomatic urethral gonorrhoea, ≥87% coverage of an N. gonorrhoeae reference genome was achieved in all samples, with ≥92% coverage breath at ≥10-fold depth in 7 (70%) samples. (ox.ac.uk)
  • Antimicrobial-resistant Neisseria gonorrhoeae is a major threat to public health and is of particular concern in the Western Pacific Region, where the incidence of gonorrhoea is high. (who.int)
  • The limit of detection for N. gonorrhoeae was 0.0256 CFU/mL for both pharyngeal and rectal specimens. (escholarship.org)
  • N. gonorrhoeae is oxidase positive (possessing cytochrome c oxidase) and catalase positive (able to convert hydrogen peroxide to oxygen). (wikipedia.org)
  • Colonies appear after 1-2 days of incubation in carbon dioxide at 35°C. They may be identified as Neisseria by demonstration of typical Gram stain morphology and a positive oxidase test result. (medscape.com)
  • INTRODUCTION & HISTORY: Discovered Neisseria gonorrhoeae (1879) Albert stain Mycobacterium lepraeAlbert Ludwig Sigesmund Neisser 3. (firebaseapp.com)
  • The major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria gonorrhoeae is essential for cell growth under oxygen limiting conditions in the presence of nitrite and is protective against killing by human sera. (rcsb.org)
  • N eisseria gonorrhoeae remains one of the most common sexually transmitted pathogens in developing and developed countries. (bmj.com)
  • More recent studies have also suggested high rates of recombination within the Neisseria genus ( Didelot & Maiden, 2010 ). (peerj.com)
  • Comparison of Gram stain with DNA probe for detection of Neisseria gonorrhoeae in urethras of symptomatic males. (firebaseapp.com)
  • We investigated if Nanopore sequencing can detect sufficient N. gonorrhoeae DNA to reconstruct whole genomes directly from urine samples. (ox.ac.uk)
  • Despite the MIC decrease (i.e., trend of improved cefixime susceptibility) during 2012-2013, the increase in the number of strains with Cfx-RS in 2014 underscores the potential threat of cephalosporin-resistant N. gonorrhoeae . (cdc.gov)
  • Title : Technological Solutions to Address Drug-Resistant Neisseria gonorrhoeae Personal Author(s) : Bristow, Claire C.;Dong, Huan;Klausner, Jeffrey D. (cdc.gov)
  • Quinolone-resistant Neisseria gonorrhoeae rates have increased worldwide since 1994. (medicaljournals.se)
  • and (ii) the factors associated with quinolone-resistant N. gonorrhoeae. (medicaljournals.se)
  • The rate of quinolone-resistant N. gonorrhoeae was 37.4% (43/115), without significant association with gender, age, sexual behaviour, past history of sexually transmitted diseases and susceptibility to other antibiotics. (medicaljournals.se)
  • The rate of quinolone-resistant N. gonorrhoeae in Paris has been increasing since 2004. (medicaljournals.se)
  • Jan 14, 2021 Fig 2: N gonorrhoeae virulence factors. (firebaseapp.com)
  • 2021. https://www.hopkinsguides.com/hopkins/view/Johns_Hopkins_ABX_Guide/540249/all/Neisseria_gonorrhoeae. (hopkinsguides.com)
  • 2021). Neisseria gonorrhoeae. (hopkinsguides.com)
  • Raw data and statistical analysis of IL-6 secretion ELISA assay of Neisseria gonorrhoeae challenged THP-1 cells expressing Siglec-5 or Siglec-14. (datadryad.org)
  • The current study reports on the analytical performance of the Abbott RealTime CT/NG assay, including the limit of detection, inclusivity, and analytical specificity for C. trachomatis and N. gonorrhoeae in rectal and pharyngeal specimens. (escholarship.org)
  • These results contribute to the understanding of the human specificity of N. gonorrhoeae and how it evolved to evade the human immune defense. (datadryad.org)
  • AMR evolution should be considered in the context of the genetic structure of the N. gonorrhoeae population. (peerj.com)
  • The DNA sequence of lsi-2 has been determined for N. gonorrhoeae 1291, a strain that expresses a high-molecular-mass LOS, and a derivative of this strain, RS132L, that produces a truncated LOS. (uky.edu)
  • The culture grew N. gonorrhoeae and was sent to the GISP laboratory for susceptibility testing. (cdc.gov)
  • In men, the best specimen for N gonorrhoeae culture is urethral exudate or urethral scrapings (obtained with a loop or special swab). (medscape.com)
  • Impact of Anatomic Site, Specimen Collection Timing, and Patient Symptom Status on Neisseria gonorrhoeae Culture Recovery. (medscape.com)
  • Surfactant-enhanced DNA accessibility to nuclease accelerates phenotypic ß-lactam antibiotic susceptibility testing of Neisseria gonorrhoeae. (bvsalud.org)