Ncbi gene silencing. Medical search. Frequent questions

It is commonly known that inactivation of certain tumor-suppressor genes occurs as a consequence of hypermethylation within the promoter regions and a numerous studies have demonstrated a broad range of genes silenced by DNA methylation in different cancer types. (nih.gov)
We found that, due to the hypermethylation of its CpG island, miR-148a undergoes methylation-mediated silencing in HCC cell lines. (spandidos-publications.com)
Epigenetic alternations in genomic DNA include cytosine methylation in CpG islands, which usually extend throughout the promoters and the first exons of genes. (spandidos-publications.com)
DNA methylation, which is associated with gene silencing ( 12 ), is carried out by DNA methyltransferases (DNMTs). (spandidos-publications.com)
Recent studies have established that, similar to mutations, methylation-mediated silencing of tumor suppressor genes plays a major role in tumorigenesis. (spandidos-publications.com)
The copy of each gene that comes from the mother is inactivated (silenced) by a mechanism called methylation. (medlineplus.gov)
This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. (tumorportal.org)
DNA methyltransferase (DNMT)-triggered DNA methylation silences gene expression. (iasp-pain.org)
Blocking this upregulation prevented nerve injury-induced DNA methylation within the promoter and 5'-untranslated region of gene, rescued expression and total Kv current, attenuated hyperexcitability in the injured DRG neurons, and alleviated nerve injury-induced pain hypersensitivities. (iasp-pain.org)
This upregulation was responsible for nerve injury-induced DNA methylation within the promoter and 5'-untranslated region of the gene, reductions in expression and Kv current and increases in neuronal excitability in the injured DRG. (iasp-pain.org)

This triggers post-transcriptional silencing through the production of short interfering RNAs (siRNAs), which target the viral RNA for degradation [ 2 ]. (biomedcentral.com)
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (appliedbioinfo.com)
MicroRNAs are non-coding, regulatory elements approximately 22 nucleotides long, with the ability to control post-transcriptional expression of genes(3). (pharmaceuticalintelligence.com)
MicroRNAs (miRNAs) are an integral part of the post-transcriptional machinery of gene expression and have been implicated in the carcinogenic cascade. (cdc.gov)

Both genes are involved in transcriptional repression and we speculate their involvement in FXN gene silencing. (frontiersin.org)
They reveal the existence of a new mechanism of TOP1/G4-dependent transcriptional repression conserved between viral and human genes. (cnrs.fr)

Through a physical interaction with the polycomb repressive complex 1 (PRC1), KDM2B targets PRC1 to CpG islands where it contributes to H2AK119ub1 and gene repression at a subset of polycomb targets. (nih.gov)
miRNAs are highly conserved, small, non-coding RNAs that negatively regulate gene expression in vertebrates through multiple mechanisms, such as complimentary base pairing with the 3′-UTR of their target mRNAs, resulting in translational repression, mRNA cleavage and mRNA decay initiated by miRNA-guided rapid deadenylation ( 10 ). (spandidos-publications.com)
Since pharmacological inhibition or genetic knockdown of DRG DNMT1 alleviated nerve injury-induced pain hypersensitivities, DRG DNMT1 contributes to neuropathic pain genesis partially through repression of DRG gene expression. (iasp-pain.org)

In normal cells, it assures the proper regulation of gene expression and stable gene silencing. (nih.gov)
CpG island elements are associated with most mammalian gene promoters, yet how they contribute to gene regulation remains poorly understood. (nih.gov)
Our analysis also suggests an interesting hypothesis that long 3′-UTRs may function as molecular buffer against gene expression regulation by individual miRNAs. (molcells.org)
We also report an interesting hypothesis that long 3′-UTRs might serve as effective molecular buffer that resists gene expression regulation by individual miRNAs. (molcells.org)
Secretory carrier membrane protein 4 (SCAMP4) was predicted to be subject to posttranscriptional gene regulation mediated by small RNA, by annotating small RNA reads mapped to exonic regions in the pig genome. (appliedbioinfo.com)
A growing body of evidence shows that lncRNAs play important functional roles in diverse biological processes, such as epigenetic regulation, cell cycle regulation, cellular growth and differentiation, by regulating the level of target genes [ 3 , 4 ]. (biomedcentral.com)

Extremely lethal consequences of IR causes double-strand breaks, which are mainly responsible for genomic instability, altered gene expression, and cell death. (biomedcentral.com)
Comparative-genomic analysis of CRISPR and cas genes leads to the hypothesis that the CRISPR-Cas system (CASS) is a mechanism of defense against invading phages and plasmids that functions analogously to the eukaryotic RNA interference (RNAi) systems. (biomedcentral.com)

It means miRNA turns off protective genes in what is called down regulating (Gene Silencing) of those genes. (ldsfreedomforum.com)
MicroRNA (miRNA) is an approximately 22-nucleotide-long single-stranded RNA that regulates its target gene expression via the RNA-induced silencing complex (RISC). (molcells.org)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (appliedbioinfo.com)
LncRNAs are involved in a wide range of regulatory mechanisms that impact gene expression, including chromatin remodeling, modulation of alternative splicing, fine-tuning of miRNA activity, and the control of mRNA translation or accumulation [ 5 ]. (biomedcentral.com)
Here we examined 145 SNPs in 6 miRNA processing genes and in 78 miRNAs which target genes known to be important in breast cancer among 906 African American (AA) and 653 European American (EA) cases and controls enrolled in the Women's Circle of Health Study (WCHS). (cdc.gov)

Fibulin-5 was silenced by promoter hypermethylation in a majority of lung cancer cell lines and primary tumors. (aacrjournals.org)
It contains a 5' CpG island that may be a frequent target of hypermethylation, and it may undergo hypermethylation-based silencing in some bladder cancers. (cancerindex.org)

The epigenetic basis for transcriptional silencing in FRDA is now well established ( 5 - 8 , 13 , 14 ). (frontiersin.org)
Collectively, these results suggest that epigenetic silencing of fibulin-5 promotes lung cancer invasion and metastasis by activating MMP-7 expression through the ERK pathway. (aacrjournals.org)
We hypothesize that highly expressed variants with distinct IGS features may induce heritable epigenetic reprogramming of the partner rDNA arrays, harmonizing the expression of thousands of genes in allopolyploids. (muni.cz)

TTC triplet repeat expansion in an intron of the nuclear FXN gene, which encodes the essential mitochondrial protein frataxin ( 1 ). (frontiersin.org)
This gene encodes a nucleolar transcriptional regulator that was first identified by its ability to bind specifically to the Myb proto-oncogene protein. (nih.gov)
Huntington disease is caused by an expanded CAG trinucleotide repeat in the gene that encodes the protein huntingtin. (medscape.com)
The founder gene mutation common among South Africans encodes an enzyme with little or no residual activity that may be lethal in homozygotes. (medscape.com)

Effects Evaluation of CDCA3 expression in OSCC derived cell lines and main OSCCs To investigate mRNA and protein expression of CDCA3 recognized being a cancer connected gene pop over to this site in our earlier microarray information, we carried out actual time quantita tive reverse transcriptase polymerase chain response and Western blot analyses applying 6 OSCC derived cell lines and major cultured human regular oral keratinocytes. (tcr-signal.com)
The geometric suggest with the SRC 1 mRNA ranges relative to the house trying to keep gene TBP in tamoxifen taken care of tumors was 1. (tcr-signal.com)
A small RNA (~22bp) that silences the expression of target mRNA. (ensembl.org)

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
Lysosomal function is critical for organismal homeostasis-mutations in genes encoding lysosomal proteins cause severe human disorders known as lysosomal storage diseases, and lysosome dysfunction is implicated in age-associated diseases including cancer, neurodegeneration and metabolic syndrome. (stanford.edu)
Variegate porphyria (VP) is an inherited disorder of porphyrin-heme metabolism arising from mutations of the gene encoding the enzyme protoporphyrinogen oxidase. (medscape.com)
To date, there have been 184 different mutations in the protoporphyrinogen oxidase gene that results in variegate porphyria. (medscape.com)

6q24-related transient neonatal diabetes mellitus is caused by the overactivity (overexpression) of certain genes in a region of the long (q) arm of chromosome 6 called 6q24. (medlineplus.gov)
Overactivity of one of the paternally expressed imprinted genes in this region, PLAGL1 , is believed to cause 6q24-related transient neonatal diabetes mellitus. (medlineplus.gov)

The third mechanism by which overexpression of genes in the 6q24 region can occur is by impaired silencing of the maternal copy of the genes (maternal hypomethylation). (medlineplus.gov)

Histone post-translational modifications near the expanded repeats are consistent with heterochromatin formation and consequent FXN gene silencing. (frontiersin.org)
By interrogating microarray data from neuronal cells treated with inhibitors of different specificity, we selected two genes encoding histone macroH2A ( H2AFY2 ) and Polycomb group ring finger 2 ( PCGF2 ) that were specifically down-regulated by the inhibitors targeting HDACs1 and 3 versus the more selective inhibitors for further investigation. (frontiersin.org)

Alternatively spliced transcript variants of this gene have been described. (tumorportal.org)
However, only one transcript has been validated in pigs, with several other transcript variants of EZH2 from digital computational analysis in NCBI that have not been empirically validated. (hindawi.com)

MicroRNA-mediated gene silencing. (albany.edu)

PMID: 31754105 ( https://www.ncbi.nlm.nih.gov/pubmed/31808382 ). (fu-berlin.de)

As one of the two distinct complexes, namely, Polycomb Repressive Complex 1 (PRC1) and PRC2, PRC2 mediates gene silencing by modulating chromatin structure [ 2 ]. (hindawi.com)

Soybean gene functions cannot be easily interrogated through transgenic disruption (knock-out) of genes-of-interest, or transgenic overexpression of proteins-of-interest, because soybean transformation is time-consuming and technically challenging. (researchsquare.com)
An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. (researchsquare.com)
However, existing virus-induced gene silencing (VIGS) and/or overexpression vectors suitable for soybean have various drawbacks that hinder their widespread adoption. (researchsquare.com)
There are three ways that overexpression of imprinted genes in the 6q24 region can occur. (medlineplus.gov)

Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
The growing volume of gene expression data in open data repositories such as the NCBI Gene Expression Omnibus (GEO) presents an opportunity to identify these gene expression similarities on a large scale across a diverse collection of datasets. (biomedcentral.com)

The WO-1 strain is known to switch between white-opaque form with higher frequency while the SC5314 strain is the strain used for gene sequence reference. (wikipedia.org)
The following dhp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
Here, we identified the coding sequence of the EZH2 gene and characterized its expression pattern in fetal tissues of Duroc pigs at 65- and 90-day postcoitus (dpc). (hindawi.com)
There were 9 amino acid insertions and an amino acid substitution in this transcript compared with the validated reference sequence in NCBI. (hindawi.com)
A single transcript chosen for a gene which is the most conserved, most highly expressed, has the longest coding sequence and is represented in other key resources, such as NCBI and UniProt. (ensembl.org)

The silencing potential of the mycorrhiza-PEBV-VIGS protocol was evaluated using two target genes PsSym19 and PsPT4 , with known mutant phenotypes. (biomedcentral.com)
The protocol produced reproducible silencing of both symbiosis genes at levels which were sufficient to produce symbiotic phenotypes. (biomedcentral.com)

In the past few decades, studies have focused on investigating the genes and proteins responsible for the development and progression of HCC ( 3 ). (spandidos-publications.com)
Polycomb Group (PcG) proteins are a family of protein complex that regulate gene expression, especially repressing gene transcription [ 1 ]. (hindawi.com)
Alternative splicing of gene can generate multiple transcripts and proteins to regulate tissue and organ development [ 17 ]. (hindawi.com)
These experimental data substantiate the theory of miRNAs being important regulators of lipoprotein receptors and transporter proteins as well as underscore the importance of employing antisense technologies to reverse their gene-silencing effects on LDL-R and ABCA1(4). (pharmaceuticalintelligence.com)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

Other Eukaryotes - 272 (source: NCBI BLink). (gifu-u.ac.jp)
Other Eukaryotes - 172 (source: NCBI BLink). (gifu-u.ac.jp)
The discovery of the elaborate and versatile systems of RNA silencing in eukaryotes is one of the pivotal advances in biology of the last decade [ 1 - 6 ]. (biomedcentral.com)

Epigeneitc silencing of ribosomal RNA genes by Mybbp1a. (nih.gov)

Genome-wide gene expression microarrays have found widespread use because of their high throughput and ability to measure the expression of tens of thousands of genes simultaneously. (biomedcentral.com)

Peripheral nerve injury upregulated DNMT1 expression in the injured DRG through the transcription factor cAMP response element binding protein-triggered transcriptional activation of gene. (iasp-pain.org)
The second query, which used a signature of silencing of the transcription factor p63 (a key regulator of epidermal differentiation), identified datasets related to stratified squamous epithelia or epidermal diseases such as melanoma. (biomedcentral.com)

TTC expansion mutation is to reduce expression of frataxin at the level of transcription ( 3 ), through the formation of heterochromatin and subsequent gene silencing ( 4 - 8 ). (frontiersin.org)
Title: Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing. (genscript.com)

The technique is valuable for research and discovery of new drugs, allowing scientists to observe and record the exact impact of individual genes on the whole biological system. (biobide.com)
Identifying similarities between patterns of differential gene expression provides an opportunity to identify similarities between the experimental and biological conditions that give rise to these gene expression alterations. (biomedcentral.com)
To demonstrate the utility of openSESAME, we used gene expression signatures of two biological perturbations to query a set of 75,164 human expression profiles that were generated using Affymetrix microarrays and deposited in GEO. (biomedcentral.com)
openSESAME is a tool for leveraging the growing body of publicly available microarray data to discover relationships between different biological states based on common patterns of differential gene expression. (biomedcentral.com)
However, a challenge for scientists performing genome-wide gene expression microarray analysis has been using these data to generate hypotheses about biological processes responsible for the patterns of differential gene expression associated with a particular trait or experimental variable. (biomedcentral.com)
One approach to this problem is to create sets of genes with common biological characteristics, such as chromosomal location, biochemical function, or observed differential expression in some experimental condition, and then to determine whether the genes in these sets are coordinately induced or repressed. (biomedcentral.com)
If a preponderance of genes in any of these predefined groups of genes (gene sets) is coordinately differentially expressed, it may be reasonable to hypothesize that the biological characteristic upon which that gene set was defined is relevant to the experimental perturbation. (biomedcentral.com)
Overall, these results will extend our understanding of the Q-type C2H2 gene family and provide valuable information for the biological breeding of sugar beet against abiotic and biotic stresses in the future. (bvsalud.org)

Homocysteine activated NLRP3 inflammasomes in THP-1-differentiated macrophages and promoted subsequent production of IL-1β and IL-18 in macrophages, which were blocked by NLRP3 gene silencing or the caspase-1 inhibitor Z-WEHD-FMK https://pubmed.ncbi.nlm.nih.gov/28394319/ High homocysteine following folic acid or vitamin B12 deficiency also down-regulates peroxisome proliferator-activated receptor (PPAR) expression. (phoenixrising.me)
Since identification of EZH2 in the research of protooncogene product Vav [ 3 ], studies have shown that EZH2 is highly expressed in tumorigenesis, which regulates the expression of tumor suppressor genes, such as in breast cancer, prostate cancer, and lung cancer [ 4 - 6 ]. (hindawi.com)

FZ-based silencing constructs induced robust silencing of phytoene desaturase genes in N. benthamiana , multiple soybean accessions, and cowpea. (researchsquare.com)
Here, the P1 protein encoded by WYMV RNA2 was shown to suppress RNA silencing in Nicotiana benthamiana. (bvsalud.org)
P1, but not G175A, interacted with gene silencing-related protein, N. benthamiana calmodulin-like protein (NbCaM), and calmodulin-binding transcription activator 3 (NbCAMTA3), and Y10A interacted with NbCAMTA3 only. (bvsalud.org)
Moreover, RNA silencing, temperature, and autophagy have significant effects on the accumulation of P1 in N. benthamiana. (bvsalud.org)

Recently it has become clear that a subset of CpG islands in embryonic stem cells can act as polycomb response elements and are recognized by the polycomb silencing systems to regulate the expression of genes involved in pluripotency and early developmental transcription programs. (nih.gov)
Unexpectedly, we also find that CpG islands are occupied by low levels of PRC1 throughout the genome, suggesting that the KDM2B-PRC1 complex may sample CpG island associated genes for susceptibility to polycomb mediated silencing. (nih.gov)

Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. (genscript.com)

Because miRNAs may regulate approximately 60% of human genes [ 11 ], the relationship between miRNAs and human diseases has been extensively explored in the last decade. (cdc.gov)

Virus-induced gene silencing (VIGS) exploits a natural defence mechanism in plants against virus infection [ 1 ]. (biomedcentral.com)

In general, microRNAs, a class of small (~21 nucleotide) non-coding RNAs, negatively regulate the expression of their target genes. (spandidos-publications.com)
There are two major, distinct forms of regulatory small RNAs involved in eukaryotic gene silencing: small interfering (si) RNAs and micro (mi) RNAs. (biomedcentral.com)
siRNAs are produced from double-stranded RNAs of viruses and transposable elements, which are processed by the dicer nuclease, one of the essential components of the RNA-Induced Silencing Complexes (RISCs) [ 7 - 9 ]. (biomedcentral.com)
MicroRNAs (miRNAs) are small, noncoding RNAs that bind to the 3' UTR of target mRNAs, and silence gene expression by inducing degradation of target mRNAs or inhibition of protein translation [ 10 ]. (cdc.gov)

The word gene editing is an umbrella term for any type of genetic engineering. (biobide.com)
Some people with this disorder have a genetic change in the maternal copy of the 6q24 region that prevents genes in that region from being silenced. (medlineplus.gov)
Genetic polymorphisms in Quantitative Trait Loci (QTL) genes such as BCL11A, HBS1L-MYB and KLF1 have been reported to influence fetal hemoglobin (HbF) levels. (mdpi.com)
This prospective study was planned to evaluate the role of genetic polymorphisms in QTL genes as determinant of HbF levels in beta thalassemia major patients. (mdpi.com)

Ankyrin Repeat and Single KH Domain 1 (ANKHD1) is a protein first discovered in Drosophila as a regulator of photoreceptor development (https://www.ncbi.nlm.nih.gov/pubmed/11782402). (sheffield.ac.uk)
Recently I uncovered that ANKHD1 controls cell proliferation by directly binding to and modulating a subset of tumour-suppressor microRNAs (https://www.ncbi.nlm.nih.gov/pubmed/29695508). (sheffield.ac.uk)

The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. (biomedcentral.com)
The development of VIGS vectors for P. sativum , G. max and P. vulgaris has paved the way for functional analysis of plant symbiosis genes in crop legumes and the transfer of knowledge from model legumes to crop plants. (biomedcentral.com)
Here our aim was to develop a "mycorrhiza-PEBV-VIGS protocol", to extend the use of VIGS for functional studies of pea genes involved in the symbiosis with AMF. (biomedcentral.com)
The new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits. (researchsquare.com)
Virus-induced gene silencing (VIGS) is an alternative functional genomics tool for soybean because it does not involve the generation of inheritable changes. (researchsquare.com)
To address this, I have received funding from the Academy of Medical Sciences (Springboard Fellowship Award - 2018-2020) to combine RNAseq transcriptomics and functional siRNA screening technologies to identify and characterize key genes involved in cystogenesis (the generation of cysts). (sheffield.ac.uk)

Pharmaceutical R&D is constantly looking into the potential effects of new molecules on specific genes with incredible precision and verifiable results. (biobide.com)
Morpholinos are oligomer molecules that are useful for modifying the expression of genes. (biobide.com)
These specialized molecules give researchers and scientists the means to block access to specific RNA and silence targeted genes for precise experimentation. (biobide.com)
Having full control over the entire process allows for in-depth research around how molecules and chemical compounds affect every aspect of a cell and its genes. (biobide.com)
The requirements for such a protocol are to establish virus infection and generation of siRNA molecules for induction of gene silencing in the pea roots before they become AMF colonized. (biomedcentral.com)

Inserting a fragment of a plant gene into a virus vector will result in a recombinant virus that triggers degradation of both the virus transcript and of homologous endogenous plant RNA sequences. (biomedcentral.com)
However, the transcript and expression status of the EZH2 gene during porcine embryonic development remains unknown. (hindawi.com)
This gene has 1 transcript ( splice variant ). (ensembl.org)

This resistance may be acquired through gene mutation. (lookformedical.com)
Variegate porphyria arises from autosomal dominant inheritance of a gene mutation encoding a defective protoporphyrinogen oxidase enzyme. (medscape.com)
Prevalence is estimated at 1 case in 300 persons in South Africa, where a protoporphyrinogen oxidase gene "founder" mutation traceable to Dutch immigrants who married there in 1680 has been widely disseminated. (medscape.com)
This relatively high prevalence compared with Europe suggests a possible protoporphyrinogen oxidase gene mutation passed down through Jewish ancestry. (medscape.com)

The diploid genome size is approximately 29 Mb, and up to 70% of the protein coding genes have not yet been characterized.C. albicans is easily cultured in the lab and can be studied both in vivo and in vitro. (wikipedia.org)
Next to this ORFeome there is also the availability of a GRACE (gene replacement and conditional expression) library to study essential genes in the genome of C. albicans. (wikipedia.org)
With a fully sequenced genome and thousands of genetically modified examples already available, these small fish are a prime example of using morpholinos for gene silencing in drug development. (biobide.com)

Lastly, we noticed an enrichment of predicted G4 sequences in the promoter of TOP1-repressed cellular genes.nnCONCLUSIONS: Our results demonstrate the formation of a TOP1/G4 complex on the HIV-1 LTR promoter and its repressive effect on the promoter activity. (cnrs.fr)
All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated ( cas ) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. (biomedcentral.com)

Characterization of the impact of the MYBBP1A gene and rs3809849 on asparaginase sensitivity and cellular functions. (nih.gov)
The progression of hepatocellular carcinogenesis is thought to involve the deregulation of genes that are critical to cellular processes, such as cell cycle control, cell growth, apoptosis, cell migration and spreading. (spandidos-publications.com)

We show here that DNMT1, a canonical maintenance methyltransferase, acts as the DNMT and is required for neuropathic pain genesis likely through repressing at least DRG gene expression in male mice. (iasp-pain.org)

Uniparental silencing of 35S rRNA genes (rDNA), known as nucleolar dominance (ND), is common in inter- speci?c hybrids. (muni.cz)

Modern gene editing makes use of incredible tools like CRISPR or morpholinos to specifically silence target genes, reducing th eir expression and changing how traits manifest in animal model organisms. (biobide.com)
This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. (tumorportal.org)
DBC1 functions as a coactivator for AR-V7 and is required for the expression of AR-V7 target genes including CDH2, a mesenchymal marker linked to CRPC progression. (cancerindex.org)
It is proposed that unique inserts of CRISPR, some of which are homologous to fragments of bacteriophage and plasmid genes, function as prokaryotic siRNAs (psiRNA), by base-pairing with the target mRNAs and promoting their degradation or translation shutdown. (biomedcentral.com)
Prime editors (PEs) are precision gene editing agents that can perform virtually any substitution, small deletion and small insertion at target DNA sites in living cells 2 . (nature.com)

These relationships may serve to generate hypotheses about the causes and consequences of specific patterns of observed differential gene expression. (biomedcentral.com)

The Knock Out Mouse Project - They took 600 mice and selectively knocked out (Silenced/switched off) singular genes and tested what diseases knocking out each gene would lead to. (ldsfreedomforum.com)

Gene silencing refers to a type of gene editing that prevents specific genes from expressing their traits. (biobide.com)
Once a gene is silenced, the other traits of the DNA and how a new drug or disease affect it are easier to see. (biobide.com)
Morpholinos allow for viable research into gene expression, connecting specific traits to specific genes. (biobide.com)

However, the viral suppressor of RNA silencing (VSR) encoded by WYMV has not been identified. (bvsalud.org)

Our work uncovers a previously unidentified role for 3'-end processing and transcription termination machinery in gene silencing. (genscript.com)

If this is done for example with some neurological protective genes or say cancer protective genes it would would remove the bodies gene security guard against those diseases. (ldsfreedomforum.com)

Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. (biomedcentral.com)
However, the available PEBV-VIGS protocols are inadequate for studying genes involved in the symbiosis with arbuscular mycorrhizal fungi (AMF). (biomedcentral.com)
Here we describe a PEBV-VIGS protocol suitable for reverse genetics studies in pea of genes involved in the symbiosis with AMF and show its effectiveness in silencing genes involved in the early and late stages of AMF symbiosis. (biomedcentral.com)

Anatomy1

Organisms3

Diseases2

Chemicals and Drugs15

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes49

Disciplines and Occupations8

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture1

Information Science32

Health Care6

Geographicals1

Methylation10

Post-transcriptional4

Transcriptional repression2

Repression3

Regulation6

Genomic2

MiRNA5

Hypermethylation2

Epigenetic3

Encodes4

MRNA3

Mutations4

Diabetes mellitus2

Maternal hypomethylation1

Histone2

Variants2

MicroRNA1

PMID1

Chromatin1

Overexpression4

Expression Omnibus2

Sequence5

Phenotypes2

Proteins5

Eukaryotes3

Ribosomal1

Thousands of genes1

TRANSCRIPTION FACTOR2

Heterochromatin2

Biological8

Regulates2

Benthamiana4

Polycomb2

RefSeq1

Human genes1

Mechanism1

RNAs4

Genetic4

Https2

Functional6

Molecules5

Transcript3

Mutation4

Genome3

Sequences2

Cellular2

Methyltransferase1

Nucleolar1

Target5

Differential gene expression1

Mice1

Traits3

Viral1

Machinery1

Neurological1

VIGS3