Myosin proteins. Medical search. Frequent questions

Protein phosphorylation plays an important role in physiological processes, such as muscle contraction. (scirp.org)
Phosphorylation of cMyBP-C is essential for normal cardiac function, since dephosphorylation of this protein leads to its degradation and has been associated with cardiomyopathy. (scirp.org)
Cohen, P. (2002) The origins of protein phosphorylation. (scirp.org)
Barefield, D. and Sadayappan, S. (2010) Phosphorylation and function of cardiac myosin-binding protein c in health and disease. (scirp.org)
Key role of myosin light chain (MLC) kinase-mediated MLC2a phosphorylation in the alpha 1-adrenergic positive inotropic effect in human atrium. (nih.gov)
The mechanical force that activates Piezo1 arises from Myosin II phosphorylation by Myosin Light Chain Kinase. (nature.com)
However, phosphorylation of C1C2 by cAMP-dependent protein kinase reduced its ability to increase Ca 2+ sensitivity. (arizona.edu)
These results demonstrate that binding of the C1C2 peptide to S2 alone is sufficient to affect myosin contractile function and suggest that regulated binding of cMyBP-C to myosin S2 by phosphorylation directly influences myofilament Ca 2+ sensitivity. (arizona.edu)
In recent years the picture that has emerged is definitely one in which cMyBP-C is definitely a key determinant of the rate and push of cardiac contraction a summary drawn from alterations in contractility that have been observed as a consequence of phosphorylation ablation or mutation of the protein. (biopaqc.com)
Cardiac contractility is regulated by dynamic phosphorylation of sarcomeric proteins by kinases such as cAMP-activated protein kinase A (PKA). (biomedcentral.com)
Efficient phosphorylation requires that PKA be anchored close to its targets by A-kinase anchoring proteins (AKAPs). (biomedcentral.com)
Cardiac Myosin Binding Protein-C (cMyBPC) and cardiac troponin I (cTNI) are hypertrophic cardiomyopathy (HCM)-causing sarcomeric proteins which regulate contractility in response to PKA phosphorylation. (biomedcentral.com)
Work done using smooth muscle myosin and mammalian non-muscle myosin have demonstrated that phosphorylation of the RLC at conserved Serine and Threonine sites ( Figure 1B , Serine-19 and Threonine-18) activates myosin motor activity, enhances the affinity of myosin for actin, and promotes myosin filament assembly ( Heissler and Sellers, 2016 ). (elifesciences.org)
However, it has not been biochemically demonstrated that Drosophila myosin motor activity and filament assembly is regulated by RLC phosphorylation or whether the extent of activation is similar to that of mammalian systems. (elifesciences.org)
They found that the locking mechanism was controlled by phosphorylation - a process where a phosphorus-based molecule is added to change the shape or action of a protein. (cosmosmagazine.com)
This is quite relevant for muscle disorders because they found that many known mutations were at, or close to, the phosphorylation sites needed to lock the protein. (cosmosmagazine.com)
One source of confusion may be that proxy measures of protein synthesis, such as mGluR-LTD or phosphorylation of signaling molecules, have been used in intact hippocampal slice preparations, whereas metabolic labeling experiments have been performed in synaptoneurosome preparations of cortex that are not easily related to altered hippocampal synaptic plasticity. (jneurosci.org)
A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. (bvsalud.org)

In its dephosphorylated state, cMyBP-C binds predominantly to myosin S2 and brakes crossbridge formation, however, when phosphorylated in response to β-adrenergic stimulation through activating cAMP-dependent protein kinase (PKA), it favours binding to actin, then accelerating crossbridge formation, enhancing force development and promoting relaxation. (wikipedia.org)
Protein kinases identified thus far to phosphorylate cMyBP-C in the M motif are PKA, Ca2+/calmodulin-dependent kinase II (CaMKII), ribosomal s6 kinase (RSK),protein kinase D (PKD), and protein kinase C (PKC). (wikipedia.org)
Furthermore, GSK3β was described as another protein kinase to phosphorylate cMyBP-C outside the M-domain in the proline-alanine-rich actin-binding site at Ser133 in human myocardium (mouse Ser131). (wikipedia.org)
One of the upstream kinases, which phosphorylate cMyBP-C, is protein kinase D (PKD). (scirp.org)
Our data suggest that elevated protein synthesis in the Fmr1 KO is due to saturation of mRNA translation downstream of the MAP kinase ERK1/2 which is basally activated by mGluR5. (jneurosci.org)
In this study, we analyzed bile canaliculi dynamics, Rho kinase (ROCK)/myosin light chain kinase (MLCK) pathway implication, efflux inhibition of taurocholate [a predominant bile salt export pump (BSEP) substrate], and expression of the major canalicular and basolateral bile acid transporters. (aspetjournals.org)
Genetic deletion of musclin reduced the nuclear abundance of protein kinase G (PKGI) and cyclic adenosine monophosphate (cAMP) response element binding (CREB), resulting in suppression of the master regulator of mitochondrial biogenesis, peroxisome proliferator-activated receptor Î³ coactivator 1α (PGC1α), and its downstream targets in response to physical activity. (bvsalud.org)

Functional divergence of human cytoplasmic myosin II: kinetic characterization of the non-muscle IIA isoform. (nih.gov)
See the other reference sequence protein isoform for the LOC373230 gene (XP_030851251.1). (beds.ac.uk)
The gene encoding the cardiac isoform of myosin binding protein-C (cardiac MyBP-C) has recently been assigned to chromosome 11p11.2 and proposed as a candidate FHC gene. (ox.ac.uk)
The slow isoform of myosin binding protein C is active during the development of skeletal muscles. (medlineplus.gov)
The mutation changes a single protein building block (amino acid) in the slow isoform of myosin binding protein C. Specifically, the mutation replaces the amino acid tryptophan with the amino acid arginine at protein position 236 (written as Trp236Arg or W236R). (medlineplus.gov)
To investigate this possibility, we assessed the ability of MMGL isoform 4 to interact with PKA regulatory subunits R1A and R2A using Y2H-based direct protein-protein interaction assays. (biomedcentral.com)

The measured FRET efficiencies were intermediate between those observed when the donor was attached to the cardiac myosin regulatory light chain in the thick filaments and troponin T in the thin filaments. (kcl.ac.uk)
2005). The binding of fessilin to smooth myosin is of particular interest because unphosphorylated smooth muscle myosin filaments are unstable in the presence of ATP (Trybus et al. (ecu.edu)
1978). However, in smooth muscle cells unphosphorylated myosin filaments are maintained (Milton et al. (ecu.edu)
2011). Several proteins have been identified that stabilize myosin filaments and actin-myosin filament interactions. (ecu.edu)
The organization of F-actin and myosin filaments by fesselin was observed by monitoring the rate of dissociation of actin-myosin by ATP in a stopped-flow device. (ecu.edu)
These experiments showed that fesselin was able to tether actin and myosin filaments together without significantly impacting the rate of the actin-myosin bond breaking. (ecu.edu)
The stabilization of myosin filaments by fesselin was tested using a similar method. (ecu.edu)
Through the stopped-flow and electron microscopy experiments it was found that fesselin stabilizes myosin filaments. (ecu.edu)
Cardiac MyBP-C is arrayed transversely in sarcomere A-bands and binds myosin heavy chain in thick filaments and titin in elastic filaments. (ox.ac.uk)
The motor domain at the N-terminus of the myosin heavy chain binds actin filaments in an ATP-dependent manner. (elifesciences.org)
The C-terminal tail of the myosin heavy chain associates with the tails of other myosin heavy chains and promotes the assembly into bipolar filaments. (elifesciences.org)
The bipolar myosin filaments promote the sliding of antiparallel actin filaments relative to one another resulting in contraction of an actin network. (elifesciences.org)
The rational for the different myosin fragments lies in the different biochemical properties: Full-length myosin forms filaments, sediments at high speed and can be used in the in vitro motility assay. (elifesciences.org)
It involves the building of a labyrinth of nano-based channels that have specific traffic regulations for protein filaments. (lu.se)
Outside the cell myosin can be used to move protein filaments, composed of actin, along artificial paths, which direct the filaments' movements. (lu.se)

MYBPC3 was thus the fourth gene for hypertrophic cardiomyopathy, following MYH7, encoding β-myosin heavy chain, TNNT2 and TPM1, encoding cardiac troponin T and α-tropomyosin, respectively, earmarking hypertrophic cardiomyopathy as a disease of the sarcomere. (wikipedia.org)
Mutations in the gene for cardiac myosin-binding protein C account for approximately 15 percent of cases of familial hypertrophic cardiomyopathy. (nih.gov)
DNA sequences encoding cardiac myosin-binding protein C were determined in unrelated patients with familial hypertrophic cardiomyopathy. (nih.gov)
eight defects (insertions, deletions, and splice mutations) were predicted to truncate cardiac myosin-binding protein C. The clinical expression of either missense or truncation mutations was similar to that observed for other genetic causes of hypertrophic cardiomyopathy, but the age at onset of the disease differed markedly. (nih.gov)
Survival was generally better than that observed among patients with hypertrophic cardiomyopathy caused by other mutations in the genes for sarcomere proteins. (nih.gov)
Schlossarek, S., Mearini, G. and Carrier, L. (2011) Cardiac myosin-binding protein c in hypertrophic cardiomyopathy: Mechanisms and therapeutic opportunities. (scirp.org)
Cardiac myosin binding protein-C (cMyBP-C) is a thick filament-associated regulatory protein frequently found mutated in patients suffering from hypertrophic cardiomyopathy (HCM). (kcl.ac.uk)
Mutations in the cardiac myosin binding protein-C gene on chromosome 11 cause familial hypertrophic cardiomyopathy. (ox.ac.uk)
Mutations in the cardiac myosin binding protein-C gene (cMyBP-C) are among the most prevalent causes of inherited hypertrophic cardiomyopathy. (arizona.edu)
In contrast hypertrophic cardiomyopathy mutations in cMyBP-C that are associated with hypercontractility actually in an individual at rest presumably induce hypercontractility by disrupting the relationships of cMyBP-C with myosin or increasing its binding to actin or both. (biopaqc.com)

Characterization of sea urchin unconventional myosins and analysis of their patterns of expression during early embryogenesis. (beds.ac.uk)
The MYO15A gene is located at chromosome 17p11.2 and contains 66 coding exons, which encodes an unconventional myosin protein Myosin XVA [ 19 ]. (hindawi.com)

Blebbing, apoptotic body formation and protein release during early apoptosis are dependent on ROCK and myosin ATPase activity to drive actomyosin contraction. (nature.com)
Many antibodies that bind to the tail of myosin-II, even some that have no obvious effect on minifilament assembly, can inhibit the actomyosin ATPase activity and the contraction of an actin gel formed in crude extracts. (duke.edu)

The myosin-binding protein C, cardiac-type is a protein that in humans is encoded by the MYBPC3 gene. (wikipedia.org)
The genotypes of these family members were determined, and the clinical status of 212 family members with mutations in the gene for cardiac myosin-binding protein C was assessed. (nih.gov)
The clinical expression of mutations in the gene for cardiac myosin-binding protein C is often delayed until middle age or old age. (nih.gov)
Delayed expression of cardiac hypertrophy and a favorable clinical course may hinder recognition of the heritable nature of mutations in the cardiac myosin-binding protein C gene. (nih.gov)
Myosin binding protein C1: a novel gene for autosomal dominant distal arthrogryposis type 1. (medlineplus.gov)
This protein encoded by this gene belongs to the WD repeat-containing family of proteins, which function in the formation of protein-protein complexes in a variety of biological pathways. (nih.gov)
The TMC1 gene is located on chromosome 9q21 and contains 24 exons that encodes a 760 amino acid membrane protein TMC1 with six transmembrane domains [ 12 , 13 ]. (hindawi.com)
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. (thebiogrid.org)
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. (thebiogrid.org)
Fragile X syndrome (FXS) is caused by loss of the FMR1 gene product FMRP (fragile X mental retardation protein), a repressor of mRNA translation. (jneurosci.org)

cMyBP-C is a myosin-associated protein that binds at 43 nm intervals along the myosin thick filament backbone, stretching for 200 nm on either side of the M-line within the crossbridge-bearing zone (C-region) of the A band in striated muscle. (wikipedia.org)
In addition to myosin, cMyBP-C also binds titin and actin. (wikipedia.org)
In this regard there is evidence (examined below) that dephosphorylated cMyBP-C preferentially binds to myosin and in so doing restricts spatial mobility of myosin and reduces the probability of myosin binding to actin. (biopaqc.com)

Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)
Researchers believe that this protein helps regulate the tensing of muscle fibers (muscle contraction). (medlineplus.gov)
The regulation of vertebrate striated muscle contraction involves a number of different molecules, including the thin-filament accessory proteins tropomyosin and troponin that provide Ca(2+)-dependent regulation by controlling access to myosin binding sites on actin. (egelmanlab.org)
One possibility is that because the N-terminus contains a unique binding site for the S2 subfragment of myosin, shortened cMyBP-C peptides could directly affect myosin contraction by binding to S2. (arizona.edu)
Cardiac myosin binding protein-C (cMyBP-C) is definitely a solid filament-associated protein that Mitragynine appears to contribute to the regulation of cardiac contraction through interactions with either myosin or actin or both. (biopaqc.com)
Although, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of actin cytoskeleton networks, recent studies have questioned the importance of myosin motor activity cell and tissue shape changes. (elifesciences.org)
Overall, our data highlights that myosin activity is required for rapid cell contraction and tissue folding in developing Drosophila embryos. (elifesciences.org)

These changes are consistent with the altered function and properties of myosin in muscle tissue exposed to oxidative stress arising from disease or from food processing. (ku.dk)
A ) Domain organization of the myosin heavy chain and myosin fragments used to study the biochemical properties of myosin. (elifesciences.org)

The approximate stoichiometry of cMyBP-C along the thick filament is 1 per 9-10 myosin molecules, or 37 cMyBP-C molecules per thick filament. (wikipedia.org)
The generation of thiyl and tyrosyl radicals is consistent with the observed consumption of cysteine and tyrosine residues, the detection of di-tyrosine by HPLC and the detection of both reducible (disulfide bond) and non-reducible cross-links between myosin molecules by SDS/PAGE. (ku.dk)
We used a series of COOH-terminally deleted recombinant myosin molecules to map precisely the binding sites of 22 monoclonal antibodies along the tail of Acanthamoeba myosin-II. (duke.edu)
This helps other molecules move the myosin around the cell for use later. (cosmosmagazine.com)

2002 In muscles cells UNC-45 guarantees proper folding of myosin Saracatinib to permit its set up and function in the sarcomere (Ao and Pilgrim 2000 Barral et al. (hiv-proteases.com)
Mutations in five different loci cause FHC and 3 disease genes have been identified: beta cardiac myosin heavy chain, alpha tropomyosin and cardiac troponin T. Because these genes encode contractile proteins, other FHC loci are predicted also to encode sarcomere components. (ox.ac.uk)
Both mutations are predicted to disrupt the high affinity, C-terminal, myosin-binding domain of cardiac MyBP-C. These findings define cardiac MyBP-C mutations as the cause of FHC on chromosome 11p and reaffirm that FHC is a disease of the sarcomere. (ox.ac.uk)
when β-adrenergic agonists were applied to the mammalian heart.8 The findings that biochemical extraction of cMyBP-C from your myofibril had no effect on the stability of the sarcomere6 and that genetic ablation of cMyBP-C in mice caused no abnormalities in sarcomere assembly9 led to the conclusion that cMyBP-C is not a structural protein at least not in the strictest sense. (biopaqc.com)
Hundreds of mutations in the genes that encode protein constituents of the sarcomere have been identified in HCM. (genome.jp)

The second position identified here would not interfere with either myosin binding or tropomyosin positioning. (egelmanlab.org)

Affinity Purified by Protein A affinity chromatography. (pp1b.com)
Antibodies that bind to the distal tail but do not affect assembly appear to have a low affinity for myosin-II. (duke.edu)
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis. (thebiogrid.org)
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. (thebiogrid.org)
Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. (thebiogrid.org)

Differential regulation of the atrial isoforms of the myosin light chains during striated muscle development. (nih.gov)
Myosin is a hexamer composed of two myosin heavy chains, two regulatory light chains (RLCs), and two essential light chains (ELCs) ( Figure 1A ). (elifesciences.org)
The light chains bind to the central neck domain of the myosin heavy chain and have structural and regulatory functions ( Heissler and Sellers, 2014 ). (elifesciences.org)
The top panel shows the myosin hexamer composed of two myosin heavy chains (green), two ELCs (light blue) and two RLCs (gray). (elifesciences.org)

Myosin binding protein C interacts with other muscle proteins, including myosin, actin, and titin. (medlineplus.gov)

cMyBP-C regulates the positioning of myosin and actin for interaction and acts as a tether to the myosin S1 heads, limiting their mobility. (wikipedia.org)

Mutation of CFAP57, a protein required for the asymmetric targeting of a subset of inner dynein arms in Chlamydomonas, causes primary ciliary dyskinesia. (nih.gov)

Actin-myosin dissociation was measured by light scattering (a measure of particle size) and by pyrene-actin or acrylodan-tropomyosin fluorescence (a measure of myosin-actin bond breaking). (ecu.edu)
One position aligns well with the previously reported binding site that clashes with the binding of myosin to actin, but would force tropomyosin into an "on" position that exposes myosin binding sites along the filament. (egelmanlab.org)
It thus appears that the ability to bind to at least two distinctly different positions on F-actin, as observed for tropomyosin, may be more common than previously considered for other actin binding proteins. (egelmanlab.org)

2005 The combined action of Hsp90 and Rng3p is vital for proper function of type II myosin in cytokinesis. (hiv-proteases.com)
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. (joplink.net)
Fission yeast Rng3p: an UCS-domain protein that mediates myosin II assembly during cytokinesis. (thebiogrid.org)

1999 UNC-45 substances display a three domains settings with an N-terminal tetratricopeptide do it again (TPR) domains (~115 proteins) a badly conserved central domains (~400 proteins) and a C-terminal UCS domains (~400 proteins) (Barral et al. (hiv-proteases.com)
A major class of proteins found in the seeds of plants and in various tissues and substances of vertebrate and invertebrate animals, including blood, muscle, and milk. (dictionary.com)
Two kinds, alpha and beta globulin, are primarily transport proteins or serve as substrates for forming other substances, and include lipoproteins and enzymes. (dictionary.com)

Motor proteins have the fun quirk of looking as though they walk along the cell's cytoskeleton to change the shape of the cell and make our muscles move. (cosmosmagazine.com)

numbering refers to the mouse sequence) are localized in the M motif of cMyBP-C and are targeted by protein kinases in a hierarchical order of events. (wikipedia.org)
However, since these kinases regulate a broad range of cellular responses, their compartmentalization in close proximity to their sarcomeric targets is required to facilitate control over which proteins are phosphorylated in response to second messenger signalling [ 8 , 9 ]. (biomedcentral.com)
At the same time, co-compartmentalization of enzymes or proteins that generate or terminate these second messenger metabolites, such as the phosphodiesterases (PDEs) which degrade cAMP and cGMP, with the relevant responsive kinases helps to optimise the precision and speed of response to second messenger signaling [ 10 ]. (biomedcentral.com)

Originally thought to have only a structural role, cMyBPC has been shown to play an important role in the regulation of cardiac contractility [ 1 ], for which the N-terminal region of the protein appears to be crucial. (biomedcentral.com)

In vitro studies showed that ligation of genetically encoded fluorophores to NcMyBP-C had no or little effect on its binding to thick and thin filament proteins. (kcl.ac.uk)

2004). The final set of experiments presented here examined the possibility that calmodulin regulates the interactions between fessilin and myosin. (ecu.edu)
The calmodulin regulation of fesselin myosin interactions would greatly expand the role that fesselin has within smooth muscle by placing it within the calcium signaling pathway. (ecu.edu)
The regulation of fesselin-myosin interactions by calmodulin was tested using pyrene labeled actin as well as N,N'-Dimethyl-N-(Iodoacetyl)-N'-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Ethylenediamine labeled fesselin (IANBD-fesselin). (ecu.edu)
These experiments showed that the interactions between fesselin and myosin are regulated by calmodulin. (ecu.edu)
These observations help to explain many of the seemingly contradictory results obtained with cMyBP-C and show how cMyBP-C can provide an additional layer of regulation to actin-myosin interactions. (egelmanlab.org)

Mechanical forces are transduced into biochemical signals by specialized proteins. (nature.com)
Here, combining the biochemical analysis of enzymatic and motile properties for purified myosin mutants with in vivo measurements of apical constriction for the same mutants, we show that in vivo constriction rate scales with myosin motor activity. (elifesciences.org)
Here, we use an in vitro assay of protein synthesis in the hippocampus of male Fmr1 knock-out (KO) mice to explore the molecular mechanisms involved in this core biochemical phenotype under conditions where aberrant synaptic physiology has been observed. (jneurosci.org)

Paralleling these changes in cardiac function, CeO2 nanoparticle treatment also diminished MCT-induced increases in right ventricular (RV) cardiomyocyte cross sectional area, ß-myosin heavy chain, fibronectin expression, protein nitrosylation, protein carbonylation and cardiac superoxide levels. (cdc.gov)

Rattus norvegicus myosin X (Myo10), mRNA. (genscript.com)

Scholars@Duke publication: Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. (duke.edu)

The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (beds.ac.uk)
These sequences represent the protein coding region of the Myo10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)

This is further improved in meat by enzymes continuing to break down proteins over time. (newscientist.com)

An interaction is detected between purified proteins in vitro. (thebiogrid.org)

Synthetic peptide corresponding to Human heavy chain Myosin/MYH3 aa 100-200 conjugated to keyhole limpet haemocyanin. (abcam.com)
The myosin motor domain, the light chain binding neck and the tail domain of the heavy chain are indicated. (elifesciences.org)

A muscle fiber contains many myofibrils , which are cylinders of muscle proteins . (howstuffworks.com)

Thankfully, the incredible detail they described provides a good model for researching these types of mutations in other myosin-related diseases. (cosmosmagazine.com)

cMyBP-C is a 140.5 kDa protein composed of 1273 amino acids. (wikipedia.org)

2002 The framework of UNC-45 as well as the identities of its interacting protein are summarized in Amount 1. (hiv-proteases.com)
2002 Saracatinib Srikakulam and Winkelmann 2004 Incomplete folding of myosin by UNC-45 works with this thesis (Liu et al. (hiv-proteases.com)

By MALDI-TOF mass spectrometry, we identified this protein to be cMyBP-C. These data were confirmed by immunostaining using the p-PKD-Ser744/748 antibody, which displayed a striated pattern similar to the one observ ed for a regular cMyBP-C antibody. (scirp.org)

Next, electron microscopy was used to support the stopped-flow data and observe the effects of fesselin on myosin filament organization. (ecu.edu)
The electron microscopy experiments further revealed that fesselin enhanced myosin filament size and organized them into bundles. (ecu.edu)
The team combined 96,000 electron microscope images to show, with remarkable detail, exactly how the myosin folds up when it is shutdown. (cosmosmagazine.com)

Abcam: antibodies, proteins, kits. (abcam.com)

2 , 3 A key feature of apoptosis has been defined as the maintenance of an intact cellular membrane (detectable as exclusion of impermeable dyes such as propidium iodide, PI) throughout the apoptotic program to prevent intracellular protein release and subsequent immunological activation. (nature.com)

Myosin XVA through its carboxy-terminal PDZ-ligand interacts with the third PDZ domain of whirlin, and then delivers whirlin to the tips of stereocilia [ 21 ]. (hindawi.com)

Experiments on the assembly of truncated myosin-II tails have revealed a complementary group of sites that participate in the assembly reactions (Sinard, J.H., D.L. Rimm, and T.D. Pollard. (duke.edu)

Anatomy8

Organisms4

Diseases1

Chemicals and Drugs27

Phenomena and Processes3

Phosphorylation18

Kinase7

Isoform6

Filaments15

Hypertrophic10

Unconventional2

ATPase2

Gene10

Binds3

Contraction8

Properties of myosin2

Molecules4

Sarcomere5

Either myosin1

Affinity5

Chains4

Titin1

Tether1

Mutation1

Tropomyosin3

Cytokinesis3

Substances3

Cytoskeleton1

Kinases3

Contractility1

Thick1

Interactions5

Biochemical3

Ventricular1

MRNA1

Recombinant1

Sequences2

Enzymes1

Vitro1

Heavy2

Myofibrils1

Mutations1

Amino acids1

20022

Antibody1

Electron3

Abcam1

Cellular1

Interacts1

Tails1