• MFE and doxorubicin exert a cytotoxic effect on human colon cancer HT-29 cell line by probably promoting or induction of apoptosis. (cellmolbiol.org)
  • Conclusion: These data demonstrate that oxaliplatin and pemetrexed synergistically interact against colon cancer cells, through modulation of cell cycle, inhibition of Akt phosphorylation, induction of apoptosis and modulation of gene expression. (worktribe.com)
  • Their importance is highlighted by the fact that they are involved in almost all essential biological cellular processes including apoptosis, differentiation, proliferation, and migration, and they are suggested to regulate more than 50% of all protein-coding genes [ 6 ]. (hindawi.com)
  • We treated TMZ-resistant cells U251-TMZ and T98G-TMZ to normoxia or hypoxia and silenced NUPR1 in hypoxia-treated U251-TMZ and T98G-TMZ cells to assess cell viability, proliferation, apoptosis, LC3-II/LC3-I and p62 expressions, and autophagic flux under different concentrations of TMZ. (techscience.com)
  • It inhibits the apoptosis of cancer cells by inhibiting caspases, and also promotes cancer cell growth by stabilizing microtubules during mitosis. (biomedcentral.com)
  • While knockout circIFT80 in HT29 cell or SW480 cells, the proliferation, and migration of the cells were inhibited, the cell cycle was arrested in G2/M phase, and the cell apoptosis was increased. (hindawi.com)
  • For example, circRNA_000166 can function as an oncogene by promoting the proliferation of CRC cells and by limiting apoptosis. (hindawi.com)
  • β -Catenin is a transcription factor that plays an important role in cell proliferation, cellular differentiation, apoptosis, and metastasis [ 10 ]. (hindawi.com)
  • Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
  • To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)
  • Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. (wjgnet.com)
  • molecular pathways leading to apoptosis or cell survival after exposure to ionizing radiation may be used in finding new and more effective strategies and modalities for the treatment of malignant tumors. (gov.rs)
  • Furthermore, silencing PrP c expression with si-PRNP amplified the fucoidan-induced changes in cell proliferation, apoptosis, and migration. (iiarjournals.org)
  • Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
  • Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
  • These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)
  • Cellular and Molecular Biology , 62 (3), 72-77. (cellmolbiol.org)
  • Cellular and Molecular Biology (CMB) is an international peer-reviewed journal in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology. (cellmolbiol.org)
  • Isolated rat colonic crypts as well as the colonic cell line HT29cll9A (HT29) were used to investigate estrogen effects on Cl' secretion and KCNQ1 channel function using a combination of electrophysiological, cellular and molecular biology and imaging techniques. (rcsi.com)
  • Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
  • Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
  • Results: The process of hormesis serves as a bridge between the biology of neuron-germ cell interactions on one hand, and the clinical relevance of these interactions on the other. (mdpi.com)
  • AIM OF THE STUDY: The purpose of this study was to evaluate the therapeutic effects of YNJ against periodontitis and its underlying molecular mechanisms. (bvsalud.org)
  • The purpose of this study was to uncover and describe molecular mechanisms of estrogen regulation of KCNQ1 channel function and its consequences for intestinal Cl' secretion, colonocyte proliferation and migration. (rcsi.com)
  • The purpose of this study is to determine whether targeting Hsp90 can alter survivin expression differently in different cancer cell lines and to explore possible mechanisms that cause the alteration in survivin expression. (biomedcentral.com)
  • Molecular characterization of this cell line revealed low expression levels and activity of Retinoblastoma protein and elevated basal levels of Erk1/2 activity and p70S6K expression and activity, which may be related to chemoresistance mechanisms. (biomedcentral.com)
  • Therefore, there is an urgent need to identify the molecular mechanisms underlying the progression of CRC. (hindawi.com)
  • Further studies are now needed to elucidate the molecular mechanisms underlying circRNA action in the development and progression of cancers. (hindawi.com)
  • Cells use a number of mechanisms to sense and maintain their homeostatic levels. (spandidos-publications.com)
  • Radiobiology focuses on the response of the cells, tissues and the organism as a whole to ionizing radiation and studying the mechanisms of radiobiological response. (gov.rs)
  • Molecular mechanisms underlying the above effects are not sufficiently known. (gov.rs)
  • In this review, we will discuss the mechanisms according to which T2D induces cellular senescence and the role of type 2 diabetes-induced cellular senescence in the pathogenesis and progression of colorectal cancer. (frontiersin.org)
  • In more recent years, findings of persistent postirradiation genomic instability, bystander effects, and other types of cellular response have posed additional questions regarding the mechanisms underlying the cytogenetic and mutagenic effects of radiation and their potential to contribute to radiation tumorigenesis. (nationalacademies.org)
  • We describe a comprehensive repository describing a collection of data from a range of studies investigating the molecular mechanisms of toxicity of glyphosate, the glyphosate-based herbicide commercial formulation Roundup, and a mixture of glypho. (diagenode.com)
  • We also performed cell viability assays, Alizarin Red and ALP staining assays, and RT-PCR to better understand how EDP affected osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). (bvsalud.org)
  • Background: The putative functions of the cellular prion protein (PrP c ) are believed to be associated with cell signaling, differentiation, survival, and cancer progression. (iiarjournals.org)
  • Expression is particularly high in the pancreas where it is thought to play a role in the differentiation of pancreatic beta cells. (embl.de)
  • To further confirm, the expression of candidate genes was investigated in exosomes derived from the parental HT-29 colorectal cancer cell line (HT-29-EXOs), and cancer stem cells (CSCs) -enriched spheroids (CSC-EXOs) derived thereof. (researchsquare.com)
  • Genetic ablation of TMEM9 inhibits colorectal cancer cell proliferation in vitro, ex vivo and in vivo mouse models. (nature.com)
  • These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)
  • MiR-133a targets FSCN1 in bladder cancer and CAV1 in head and neck squamous cell carcinoma functioning as a tumor suppressor [ 9 , 10 ]. (hindawi.com)
  • Oral squamous cell carcinoma (OSCC) is a common malignant tumor. (techscience.com)
  • Characterization of exosomes isolated from HeLa cells was performed by transmission electron microscopy, zeta sizer, and western blotting. (techscience.com)
  • In the 1970s, somatic cell genetic techniques were developed to allow the quantification and characterization of specific gene mutations arising in irradiated cultures of somatic cells. (nationalacademies.org)
  • Mcsweegan E. , Walker R. I. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. (microbiologyresearch.org)
  • Cell proliferation studies were performed by MTT assays and cell cycle analyses were performed by flow cytometry. (biomedcentral.com)
  • Relative cell viability was assessed using MTT assays. (wjgnet.com)
  • The migratory and invasive phenotypes of CRC cells were evaluated by in vitro transwell assays and in vivo metastatic models. (thno.org)
  • Subsequently, in-cell assays were performed to test the inhibitory potency of our compounds by evaluating their cytotoxicity in co-administration with the reference chemotherapeutic agents. (ebris.eu)
  • Lower DRI values may protect normal cells while selectively destroying tumor cells (Chou 2010). (pi3k-inhibitors.com)
  • In this present study, we found that circIFT80 was abnormally overexpression in colorectal cancer tissues and tumor cells. (hindawi.com)
  • We explored molecular markers shared between TDEs and circulating tumor cells (CTCs) in the blood of cancer patients to identify candidate genes involved in CRC metastasis. (researchsquare.com)
  • Tumor-derived EV modulates cellular activities in recipient cells by transferring genetic information from cancer cells. (researchsquare.com)
  • Essential oil prepared by distillation of the gum resin traditionally used for aromatic therapy has also been shown to have tumor cell-specific anti-proliferative and pro-apoptotic activities. (biomedcentral.com)
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • Anti-invasive and anti-multicellular tumor properties were evaluated by cellular network and spheroid formation models, respectively. (biomedcentral.com)
  • Through the Molecular Docking studies, the research team of the Oncotherapy Project of the Ebris Foundation designed a library of potentially active molecules on a protein belonging to the family of MDR (Multi Drug Resistance proteins) known to be expressed in numerous resistant tumor forms. (ebris.eu)
  • In particular, a protocol for the preparation of 3D cell cultures in micro-fluidics was developed in the laboratories of the Ebris Foundation, which induces the production of extracellular matrix (ECM) resulting from an active interaction between tumor cells and stromal cells, mimicking what happens in vivo. (ebris.eu)
  • Tumor growth will be monitored and cellular system death due to drug action will be observed. (ebris.eu)
  • Finally, platinum-DNA adduct levels were detected by ultra-sensitive multi-collector inductively coupled plasma mass spectrometry (ICP-MS). Results: A dose-dependent inhibition of cell growth was observed after drug exposure, while a synergistic interaction was detected preferentially with sequential combinations. (worktribe.com)
  • Interestingly, real-time PCR and translation inhibition studies revealed that survivin was over-expressed partially through the up-regulation of protein translation instead of gene transcription in A549 cancer cells. (biomedcentral.com)
  • In addition, 17-AAG-treated A549, HONE-1 and HT-29 cells showed reduced proteasomal activity while inhibition of 26S proteasome activity further increased the amount of survivin protein in cells. (biomedcentral.com)
  • Autophagic cell death was excluded as the inhibition of autophagy did not attenuate the cytotoxicity. (spandidos-publications.com)
  • Treatment of CRC cells with MS-444 resulted in growth inhibition and increased apoptotic gene expression, while similar treatment doses in non-transformed intestinal cells had no appreciable effects. (oncotarget.com)
  • Conclusion: Combination of fucoidan with silencing of PrP c has a synergic effect on the inhibition of HT29 colon cancer cell growth. (iiarjournals.org)
  • Activation of AMPK by low energy supply and phosphorylation of LKB1 results in an inhibition of mTOR , thus decreasing energy-consuming processes, in particular translation and, thus, cell growth. (sdbonline.org)
  • Materials and Methods: PrP c expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. (iiarjournals.org)
  • Results: Fucoidan treatment significantly inhibited growth and reduced cyclin and cyclin-dependent kinase (CDK) expression in HT29 colon cancer cells. (iiarjournals.org)
  • Methods: Human HT29, WiDr, SW620 and LS174T cells were treated with oxaliplatin and pemetrexed. (worktribe.com)
  • MATERIALS AND METHODS: The chemical components of EDP were analyzed by UHPLC-MS. The hub target and pathway enrichment analysis was conducted using molecular docking or network pharmacology. (bvsalud.org)
  • We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
  • Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
  • H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
  • We have determined that the ability of C. jejuni to bind to epithelial cell lines in vitro is significantly affected by the growth temperature and growth stage of the bacteria, but not by growth-medium composition. (microbiologyresearch.org)
  • Recently, the first in vitro norovirus cell culture model was reported for a virus that infects mice ( 18 , 19 ). (cdc.gov)
  • Instead, targeting Hsp90 with small molecule inhibitors will induce the over-expression of survivin in certain cancer cell lines and subsequently enhances the ability of cell survival in drug-treated situations. (biomedcentral.com)
  • This chapter considers the general aspects of dose-response relationships for radiobiological effects and subsequently reviews the largely cellular data on a range of radiobiological end points. (nationalacademies.org)
  • Unfortunately, the molecular mechanism manipulating metastasis in CRC is still elusive. (thno.org)
  • Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
  • γ-Secretase cleavage of these sorting receptors is inhibited by γ-secretase inhibitors and does not occur in PS1/PS2 knockout cells. (biomedcentral.com)
  • Knockout of TRDMT1 methyltransferase affects DNA methylome inglioblastoma cells. (diagenode.com)
  • We conclude that (i) SCFA potently stimulate ANGPTL4 synthesis in human colon adenocarcinoma cells and (ii) SCFA transactivate and bind to PPARγ. (uea.ac.uk)
  • QUE significantly improves the cytotoxicity of ROT + G0 6983 ranged 30-55% and PI-103 ranged 24-63% after 24-48 h against MCF-7 cells. (pi3k-inhibitors.com)
  • Additionally in the presence of QUE, the improved cytotoxicity of ROT + G0 6983 is observed to range 69-75% and PI-103 ranged 45-88% after 24-48 h in RAW 264.7 cells. (pi3k-inhibitors.com)
  • The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)
  • Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
  • Cell death Doxorubicin Flavonoid Viability. (cellmolbiol.org)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • It has been proposed that its abilities to adhere to and enter epithelial cells represent properties essential to virulence. (microbiologyresearch.org)
  • However, the characteristics of these interactions and factors that may influence the association of C. jejuni with epithelial cells are incompletely described. (microbiologyresearch.org)
  • Studies of viral attachment to cultured gastrointestinal epithelial cells (Caco-2) using recombinant virus-like particles or infectious noroviruses indicate that specific histo-blood group antigens play a key role in the attachment of the virus to the host cells ( 13 - 17 ). (cdc.gov)
  • In this study, exosomes released from HeLa cervical cancer cells were loaded with raloxifene to increase its bioavailability, and an aptamer was attached to the exosome membrane for targeting only HeLa cells. (techscience.com)
  • Fauchere J. L. , Rosenau A. , Veron M. , Moyen E. N. , Richard S. , Pfister A. Association with HeLa cells of Campylobacter jejuni and Campylobacter coli isolated from human feces. (microbiologyresearch.org)
  • Fauchere J.-L. , Kervella M. , Rosenau A. , Mohanna K. , Veron M. Adhesion to HeLa cells of Campylobacter jejuni and C coli outer membrane components. (microbiologyresearch.org)
  • Molecular phenotypes of clinical samples have proven useful in disease diagnosis, patient stratification, and drug discovery. (molcells.org)
  • However, the underlying molecular mechanism of YNJ in the periodontitis treatment is not well understood. (bvsalud.org)
  • The molecular mechanism of YNJ were predicted using network pharmacological analysis and validated using immune-staining and Western blot. (bvsalud.org)
  • This study investigated the mechanism of NUPR1 in TMZ resistance in hypoxia-treated glioma cells and its mechanism in modulating autophagy. (techscience.com)
  • Our previous findings suggest the extensive mechanism of quercetin (QUE) mediated regression of cell survival, cell proliferation, oxidative stress, inflammation, and angiogenesis via modulating PI3K and PKC signaling in lym- phoma as well as hepatocellular carcinoma. (pi3k-inhibitors.com)
  • Familial CRC, caused by hereditary mutations, is the type of cancer that neither its molecular mechanism is precisely known nor cannot be classified as a specific hereditary cancer ( Stoffel and Kastrinos, 2014 ). (frontiersin.org)
  • In contrast to the influx of amino acids, the degradation of proteins is also the important intracellular mechanism for releasing free amino acids both under steady-state conditions and during cellular stresses. (spandidos-publications.com)
  • Drug interaction was studied using the combination index method, while cell cycle was investigated with flow cytometry. (worktribe.com)
  • Amino acids are essential for cellular homeostasis, growth and proliferation via their contribution to a diverse range of cellular processes. (spandidos-publications.com)
  • Age-related and metabolic diseases such as type 2 diabetes (T2D) represent a source of cellular stress due to their disruptive effect on normal physiological processes and, therefore, can induce premature senescence ( 10 ). (frontiersin.org)
  • Vesicular acidification and trafficking are associated with various cellular processes. (nature.com)
  • The master kinase LKB1 is a key regulator of several cellular processes, including cell proliferation, cell polarity and cellular metabolism. (sdbonline.org)
  • For clinical samples, the general purpose of gene set analysis of genome-wide expression profiles is to identify underlying disease-associated molecular processes, which can facilitate disease diagnosis and therapeutic intervention. (molcells.org)
  • Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • In addition to the realization of cell cultures in 3D, the collected tissues were fragmented and stored in cryogenic tubes in order to set up a bio-bank of tissues to be used both in view of the application of protocols to create organoid cultures from frozen tissues and through flash frozen technique, for genomic and proteomic sequencing. (ebris.eu)
  • Cells grown on porous collage-coated beads under fluid shear conditions in rotating wall vessel bioreactors differentiate into 3-D architectures resembling both the morphologic and physiologic function of in vivo tissues. (cdc.gov)
  • Capsaicin (10-100 µM) significantly inhibited testosterone-treated WPMY-1 cell growth at 48 h by MTT assay. (techscience.com)
  • This study establishes a role for estrogen in the regulation of colonic electrolyte secretion via modulation of KCNQ1 cell membrane surface abundance and KCNQ1:KCNE3 complex formation. (rcsi.com)
  • In fact, several studies have shown that T2D induces senescence in multiple types of cells, including fibroblasts and endothelial cells ( 11 , 12 ). (frontiersin.org)
  • We predict that HBx/miR-221-mediated CXCL12/CXCR4 signaling induces NKT cells to promote HBV-related HCC. (techscience.com)
  • In this study, the response of malignant and healthy cells on various modalities of ionizing radiation is explored in order to improve knowledge of pathways of cell death and survival. (gov.rs)
  • Distinct pathways of cell migration and antiapoptotic response to epithelial injury: structure-function analysis of human intestinal trefoil factor. (embl.de)
  • The mechanistic basis for the biological properties of Morus alba flavonoid extract (MFE) and chemotherapy drug of doxorubicin on human colon cancer HT-29 cell line death are unknown. (cellmolbiol.org)
  • Here, we demonstrated that Hsp90 inhibitors, geldanamycin and 17-AAG, induced the over-expression of survivin in three different human cancer cell lines as shown by Western blotting. (biomedcentral.com)
  • We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
  • Here, we show that transcription and secretion of ANGPTL4 in human T84 and HT29 colon adenocarcinoma cells is highly induced by physiological concentrations of short-chain fatty acids (SCFA). (uea.ac.uk)
  • At concentrations required for PPARγ activation and ANGPTL4 induction in colon adenocarcinoma cells, SCFA do not stimulate PPARγ in mouse 3T3-L1 and human SGBS adipocytes, suggesting that SCFA act as selective PPARγ modulators (SPPARM), which is supported by coactivator peptide recruitment assay and structural modeling. (uea.ac.uk)
  • The expression of ELF4 in human CRC samples and CRC cell lines was determined by quantitative real-time PCR, immunohistochemistry and immunoblotting. (thno.org)
  • To understand the role of autophagy in the pathogenesis of glial tumors in vivo, an established Drosophila melanogaster model of glioma was used based on overexpression in larval glial cells of an active human EGFR and of the PI3K homolog Pi3K92E /Dp110. (sdbonline.org)
  • In contrast, internalisation is markedly diminished at low temperature, appears to involve active invagination of the target cell membrane via pseudopod formation, and is maximal when cells of human origin are employed. (microbiologyresearch.org)
  • Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed. (cdc.gov)
  • However, these models do not answer the fundamental questions of human norovirus attachment and entry into cells of the human gastrointestinal tract. (cdc.gov)
  • Representative models of differentiated human intestinal epithelium can be established by growing cells in 3 dimensions (3-D) on collagen-I-coated porous microcarrier beads in rotating-wall vessel (RWV) bioreactors that model the physiologic fluid-shear environment in their respective organs ( 21 - 24 ). (cdc.gov)
  • PI3K-PKC pathway is a key monitor of mammalian cells regulated by its different isoenzymes, which may exert similar or opposite cellular effects by differential coupling of signaling pathways. (pi3k-inhibitors.com)
  • Therefore, we hypothesized the improved anticancer efficacy of QUE in combination with isoenzyme inhibitors-rottlerin (ROT-PKCδ inhibitor), G0 6983 (PKCα inhibitor), and PI-103 (p110α-class I PI3K inhibitor) in MCF-7 and RAW 264.7 cells. (pi3k-inhibitors.com)
  • Overall results of the current study suggested that QUE improves the synergistic anticancer efficacy in combination with PI-103, ROT, and G0 6983 in MCF-7 and RAW 264.7 cells. (pi3k-inhibitors.com)
  • 2015). Our previous finding entails the QUE-mediated regression of cell survival, cell proliferation, oxidative stress, inflammation, and angiogenesis in lympho- ma as well as hepatocellular carcinoma via modulating PI3K and PKC signaling (Maurya and Vinayak 2015a, b, c, 2016a, 2017a). (pi3k-inhibitors.com)
  • FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
  • The study of this discipline not only is constantly unveiling specific molecular signaling which the onset and progression of cancer can be advocate for, but also offer the tremendous opportunity for a pharmacogenomics-driven specific approach. (techscience.com)
  • With the advancement and affordability of new generation sequencing and molecular signaling monitoring, an ever increasing number of cancer pathways are nowadays under scrutiny. (techscience.com)
  • Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
  • Molecular 'switch' reverses chronic inflammation and aging - Science Daily, 2/6/20 - 'By studying mice and immune cells called macrophages, the team found that a protein called SIRT2 is responsible for deacetylating the NLRP3 inflammasome. (qualitycounts.com)
  • Another consequence of aging is the accumulation of so-called senescent cells, normal cells that stop dividing, contribute to tissue aging and secrete substances like cytokines that induce inflammation. (qualitycounts.com)
  • Elimination of senescent cells can counter chronic inflammation, said Steven N. Austad, director of aging studies at the University of Alabama at Birmingham. (qualitycounts.com)
  • Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)
  • This age-related inflammation, also referred to as inflammaging, increases the risk for tissue damage and genetic aberrations that cause cellular transformation and cancer development ( 8 , 9 ). (frontiersin.org)
  • 2015). Therefore, effective che- motherapeutic drugs require multiple molecular/cellular tar- gets/pathways to control cancer cell survival/proliferation. (pi3k-inhibitors.com)
  • The effect of doxorubicin and flavonoid extract on colon cancer HT-29 cell line death and identification of APC gene expression and PARP concentration of HT-29 cell line were investigated. (cellmolbiol.org)
  • The results showed that flavonoid extract and doxorubicin induce a dose dependent cell death in HT-29 cell line. (cellmolbiol.org)
  • A prostatic stromal myofibroblast cell line (WPMY-1) was co-incubated with testosterone (1 µM) and different concentrations of capsaicin (10-100 µM) for 24 and 48 h. (techscience.com)
  • For example, over-expression of survivin has been shown to induce drug resistance against anti-mitotic compounds by stabilizing microtubule network in vincristine/colchicine-resistant oral cancer cells and down-regulation of it restores drug sensitivity to those compounds in the same cell line [ 9 ]. (biomedcentral.com)
  • Using cell culture techniques, we isolated clonal populations from primary cultures of ascitic effusion derived from a colon cancer patient and after several passages an established cell line, HGUE-C-1, was obtained. (biomedcentral.com)
  • We have established a new cell line from ascitic efussion of a colon cancer patient who did not respond to 5-fluorouracil or irinotecan. (biomedcentral.com)
  • The HGUE-C-1 cell line was sensitive to erlotinib, gefitinib, NVP-BEZ235, rapamycin and trichostatin, among other drugs, but partially resistant to heat shock protein inhibitors and highly resistant to AZD-6244 and oxaliplatin, even though the patient from which this cell line was derived had not been exposed to these drugs. (biomedcentral.com)
  • The novel colon cancer cell line HGUE-C-1 was obtained from ascitic efussion of a 76-year old man with colon cancer. (biomedcentral.com)
  • Then we constructed a stable HBV-HCC cell line. (techscience.com)
  • BTC was initially described, purified and cloned from a mouse insulinoma cell line. (embl.de)
  • However, cellular clearance of autophagic cargoes appears inhibited upstream of autophagosome formation. (sdbonline.org)
  • Because age-related cellular senescence and type 2 diabetes (T2D) have been recognised as risk factors for CRC development, the recent finding that type 2 diabetic patients present an elevated circulating volume of senescent cells raises the question whether type 2 diabetes facilitates the process of CRC tumorigenesis by inducing premature cell senescence. (frontiersin.org)
  • Heat shock protein 90 (Hsp90) is a molecular chaperone that assists the correct folding and stabilization of various proteins in cells. (biomedcentral.com)
  • from different cell types have different effect on adherence The fine structure of the GAGs differs according not only to their nature, but also to the developmental phase Pyruvate dehydrogenase and the physiological and pathological conditions as well as to the cellular type. (pafr-inhibitor.com)
  • We found that hypoxia upregulated NUPR1 expression and autophagy while NUPR1 silencing suppressed hypoxia-induced TMZ resistance and autophagy in glioma cells. (techscience.com)
  • BACKGROUND: Immune evasion in glioblastoma (GBM) shields cancer cells from cytotoxic immune response. (diagenode.com)
  • More abundant high molecular weight compounds, including boswellic acids, were present in Boswellia sacra essential oil prepared at 100 o C hydrodistillation. (biomedcentral.com)
  • Since the molecular chaperone Hsp90 binds and stabilizes survivin, it is widely believed that down-regulation of survivin is one of the important therapeutic functions of Hsp90 inhibitors such as the phase III clinically trialed compound 17-AAG. (biomedcentral.com)
  • The present study demonstrates that HBx promotes HCC cell proliferation via the C-X-C motif chemokine ligand 12-C-X-C chemokine receptor type 4 (CXCL12-CXCR4) axis. (techscience.com)
  • The present study evaluates the pharmacological interaction between oxaliplatin and pemetrexed in colorectal cancer cells. (worktribe.com)
  • Increased survivin mRNA transcripts were observed in 17-AAG and geldanamycin-treated HT-29 and HONE-1 cancer cells. (biomedcentral.com)
  • At the functional level, down-regulation of survivin by siRNA further increased the drug sensitivity to 17-AAG in the tested cancer cell lines. (biomedcentral.com)
  • Interestingly, a recent report suggests that over-expression of survivin may also enhance DNA double-strand breaks (DBD) repair capability in radiation-treated oral cancer cells by up-regulating the molecular sensor of DNA damage, Ku70 [ 11 ]. (biomedcentral.com)
  • Targeting specific pathways which are aberrant in cancer cells relative to normal cells is ideally a good approach at therapeu- tic dosages. (pi3k-inhibitors.com)
  • Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
  • BACKGROUND: Spermatogonial stem cell transplantation (SSCT) is proposed as a fertility therapy for childhood cancer survivors. (diagenode.com)
  • Gene expression profiling is probably the most accessible strategy for molecular phenotyping of clinical samples. (molcells.org)
  • Genetic analysis of HGUE-C-1 cells was performed by PCR of selected exons and sequencing. (biomedcentral.com)
  • Under AAS culture conditions, the cell killing effect of GEF was synergistically pronounced in the EGFR-expressing cell lines, namely, CAL 27, Detroit 562, A549 and PANC-1 cells compared with those treated with either GEF or AAS alone. (spandidos-publications.com)
  • They therefore selected and synthesized, using our chemical and pharmaceutical skills, a series of molecules on which we performed proteomics experiments (DARTs) to confirm the selected target, choosing some cell lines resistant to chemotherapy such as mitoxantrone (including HepG2, MCF7-transfected and HT29). (ebris.eu)
  • DNA chip technology and RNA sequencing have been widely used for molecular profiling of patient-derived primary cells and cell lines. (molcells.org)
  • Unlike other IAPs, survivin is a bifunctional protein that functions as a key regulator of mitosis and inhibitor of programmed cell death. (biomedcentral.com)
  • Purpose: We have previously shown that TRDMT1 methyltransferase is a regulator of chemotherapy-associated responses in glioblastoma cells. (diagenode.com)
  • Endocannabinoids can affect multiple cellular targets, such as cannabinoid (CB) receptors, transient receptor potential cation channel, subfamily V, member 1 (TRPV1) and peroxisome proliferator-activated receptor ${\gamma}$ ( $PPAR{\gamma}$ ). (koreascience.kr)
  • 2013). Same combination in polymeric micelles reduced the doxorubicin-induced cardiotoxicity in SKOV-3 and H9C2 cells as well as in mice, suggesting to limit the adverse effects of chemotherapy (Cote et al. (pi3k-inhibitors.com)
  • The good herb: Buckwheat liquor helps cells clean house - Science Daily, 8/16/21 - 'When the researchers looked more closely at specific components of tartary buckwheat extract, they found that one component, quercetin, had the same effects as the extract. (qualitycounts.com)
  • Since the early years of radiobiology the cellular effects of ionizing radiation have been studied in the context of induced chromosomal aberrations, and early models of radiation action were largely based upon such studies (Savage 1996). (nationalacademies.org)
  • The main focus of the review is the issue of cellular effects at low doses of low-LET (linear energy transfer) radiation. (nationalacademies.org)
  • The forskolin-stimulated Cl' secretion and KCNQ1 current in rat colon and HT29 epithelia were rapidly reduced following estrogen treatment (lOnM) and remained inhibited over 2 hours after estrogen exposure. (rcsi.com)