Molecular gene transfer techniques. Medical search. Frequent questions

It unravels the secrets of genes, which are segments of genetic material, such as DNA, containing instructions for building proteins and controlling various cellular processes. (freescience.info)
By investigating nucleic acids like DNA and RNA, as well as proteins and other biomolecules, scientists gain insights into gene expression, protein synthesis, gene transfer, and more. (freescience.info)
By studying genes, proteins, and other molecules within cells, biologists can identify genetic mutations responsible for inherited disorders. (freescience.info)
The UGT1 family constitutes a complex gene locus on human chromosome 2q37 and comprises 13 first exons that encode the unique N-terminal domains of the UGT1A proteins and exons 2 to 5 that encode the C-terminal domain, which is identical in all UGT1A family members ( Owens and Ritter, 1992 ). (aspetjournals.org)
This process allows a variety of important applications, including the study of gene regulation, the analysis of the expression of proteins within eukarotic cells such as those of mammals, the production of transgenic organisms , and the methods of gene therapy. (newworldencyclopedia.org)
The ABC superfamily of genes encodes proteins that transport a number of substrates across cell membranes. (medscape.com)

In molecular biology, generalized transduction is routinely used as a technique to manipulate and construct bacterial strains. (bvsalud.org)
Recent projects have included the molecular engineering of viruses, and the mechanisms of bacterial pathogenicity and host colonisation. (reading.ac.uk)

Using human arginase deficiency, a defect in the urea cycle, as a model system, Grody and his colleagues are exploring, in close collaboration with the laboratory of Dr. Stephen Cederbaum, the molecular structure and tissue-specific regulation of the arginase genes in health and disease. (uclahealth.org)
Structure and Tissue-Specific Regulation of UGT Genes (P.I.M., P.A.G., Y.I., A.J.H. (aspetjournals.org)

Targeted molecular therapy, like therapy with monoclonal antibodies, gene therapy, and other therapies, has limited or nonexistent side effects on normal cells of the body, unlike present modalities such as surgery, chemotherapy, and radiotherapy. (medscape.com)
Targeted molecular therapy can also act as a complement to other existing cancer therapies. (medscape.com)
and (3) the use of gene therapies that specifically target germ line cells for eugenic purposes. (lifeissues.net)
Second, there have been scientific studies documenting the fact that not only the somatic cells but also the germ line cells of the patient can be accidentally infected in the process of using somatic cell gene therapies. (lifeissues.net)
Of even more concern would be the use of somatic cell gene therapies that are purposefully and deceptively used to change the genetic makeup of the patient's future progeny who would continue to carry the "foreign" gene - all done under the guise of treating the somatic cells of a particular patient to "cure" his/her disease. (lifeissues.net)
Gene therapies are being used to treat a small number of diseases, including an eye disorder called Leber congenital amaurosis and a muscle disorder called spinal muscular atrophy . (medlineplus.gov)
Many more gene therapies are undergoing research to make sure that they will be safe and effective. (medlineplus.gov)
Our data demonstrate that HSV-TK/GCV suicide gene therapy system is effective for treating experimental human pancreatic cancer, which is largely resistant to the common therapies, so the suicide gene therapy system may be a potential treatment approach for pancreatic cancer. (wjgnet.com)

The procedure, in which bone marrow cells are genetically modified outside the body and re-implanted, was previously thought to avoid creating infectious viruses and causing cancer, both recognized major hazards of gene therapy. (i-sis.org.uk)
The comments, "it is extremely unlikely that genes from genetically modified (GM) food can end up in bacteria in the gut of people who eat them", and "the findings had been assessed by several Government experts who had ruled that humans were not at risk", are seriously misleading. (i-sis.org.uk)
On the other hand, genetically modified seeds (and hence the transgenic) differ from hybrids because the latter are a result of the selection of the stems of plants with desirable characteristics (for example, the large size of the fruit), but this selection of genes are those that appear naturally in populations of plants and not imported from organisms of other species, as is the case with the gm. (semillasdevida.org.mx)

With the increased understanding of molecular mechanisms and basic pathways in the pathogenesis of squamous cell cancer of the head and neck , these pathways may be modified, and rational approaches in cancer therapy at the molecular level may be created. (medscape.com)
The goal of specific molecular targets in cancer therapy is to create a "magic bullet" that selectively kills cancer cells. (medscape.com)
Targeted molecular therapy against EGFR has shown promise as an adjuvant therapy in preliminary studies in several solid tumors, including head and neck cancer. (medscape.com)
Various techniques have been developed for targeting cancer cells: gene therapy, monoclonal antibodies (MAbs), antibody toxin conjugates, small-molecule inhibitors, antisense molecules, and tumor vaccines. (medscape.com)
The goal of gene therapy is to introduce new genetic material into cancer cells that selectively kills them without causing toxicity to the surrounding cells. (medscape.com)
This is called "recombinant-DNA somatic cell gene transfer" or therapy, the point being that the targets are the diseased somatic cells of the patient. (lifeissues.net)
Yet there has been little discussion of gene therapy and its "pros" and "cons" even among scientists, much less in the public square. (lifeissues.net)
Alternatives for gene replacement therapy have been explored by re-directing the transfected genes to different subcellular compartments. (uclahealth.org)
For example, gene therapy offers promising solutions by delivering functional genes or gene-editing tools directly into affected cells. (freescience.info)
What is gene therapy? (medlineplus.gov)
Gene therapy is a medical approach that treats or prevents disease by correcting the underlying genetic problem. (medlineplus.gov)
Gene therapy techniques allow doctors to treat a disorder by altering a person's genetic makeup instead of using drugs or surgery. (medlineplus.gov)
Later studies led to advances in gene therapy techniques. (medlineplus.gov)
MedlinePlus offers a list of links to information about genes and gene therapy . (medlineplus.gov)
The Genetic Science Learning Center at the University of Utah provides an interactive introduction to gene therapy and a discussion of several diseases for which gene therapy has been successful . (medlineplus.gov)
The Centre for Genetics Education provides an introduction to gene therapy and other therapeutics , including a discussion of ethical and safety considerations. (medlineplus.gov)
The National Heart, Lung, and Blood Institute describes the approaches to gene therapy and how they work . (medlineplus.gov)
Your Genome from the Wellcome Genome Campus provides an introduction to gene therapy . (medlineplus.gov)
KidsHealth from Nemours offers a fact sheet called Gene Therapy and Children . (medlineplus.gov)
The report covers research from the authors' laboratories on the structure and regulation of UDP-glucuronosyltransferase (UGT) genes, glucuronidation of xenobiotics and endobiotics, the toxicological relevance of UGTs, the role of UGT polymorphisms in cancer susceptibility, and gene therapy for UGT deficiencies. (aspetjournals.org)
Interindividual UGT variability likely plays an important role in drug efficacy and xenobiotic toxicity, as well as in hormonal regulation and certain diseases, which in some cases may be amenable to therapeutic manipulations including gene therapy. (aspetjournals.org)
Your Genome from the Wellcome Genome Campus provides an introduction to gene therapy and describes several techniques . (medlineplus.gov)
The risk of cancer is highlighted by the recent report that gene therapy - genetic modification of human cells - claimed its first cancer victim [4]. (i-sis.org.uk)
To investigate the in vitro effects of suicide gene therapy system of herpes simplex virus thymidine kinase gene (HSV-TK) in combination with the treatment of nucleotide analog-ganciclovir (GCV) on human pancreatic cancer, and to provide a novel clinical therapeutic method for human pancreatic cancer. (wjgnet.com)
Using his knowledge of how genes are organized and repaired in human cells, Dr. Graham Dellaire, Dalhousie Medical School's Cameron Research Scientist in Cancer Biology, has developed a technique that could make gene therapy more effective and safer to use. (genetherapynet.com)
Iranian researchers of Tabriz University of Medical Sciences managed to apply laboratory synthesis of nano-polymeric carriers which can be used in gene therapy of cancer cells. (genetherapynet.com)
Gene therapy is an experimental technique through which defective genes are modified and sent into the cell by a carrier. (genetherapynet.com)
Cell and Gene Therapy Research Projects Leader, Head of Transgene Discovery, and Head of Transgene Delivery. (genetherapynet.com)
Gene Therapy Net is the information resource for basic and clinical research in gene therapy, and the site serves as a network in the exchange of gene therapy information and breaking news items. (genetherapynet.com)
Advertising opportunities on Gene Therapy Net include standard size banners as well as text ads. (genetherapynet.com)
You can also make a donation by PayPal and support the Gene Therapy Net website. (genetherapynet.com)
Molecular Therapy - Methods and Clinical Development. (lu.se)
As of first of January 2022, StemTherapy and MultiPark have decided to merge the former iPSC, CRISPR and vector platforms into the new Cell and Gene Therapy Core . (lu.se)
The Cell and Gene Therapy core is an open-access infrastructure and our services include AAV and LV vector production, cloning services, iPS reprogramming, iPS-edits and CRISPR experimental designs. (lu.se)

Genes encoding anti-fungal compounds are now being transferred to important commercial cultivars.The hypothesis that transgenic rootstocks can be used to transmit gene products to scions via the graft union is being tested in ongoing research. (cornell.edu)
Horizontal gene transfer is one of the most serious, if not the most serious hazard of transgenic technology. (i-sis.org.uk)
Transgenic DNA is in many respects optimised for horizontal gene transfer. (i-sis.org.uk)
Transgenic constructs contain new combinations of genes that have never existed, and they also amplify gene products that have never been part of our food chain [3]. (i-sis.org.uk)
Similarly, I was not surprised by the research results released earlier this year by the Food Standards Agency [12], indicating that transgenic DNA from GM soya flour, eaten in a single hamburger and milk shake meal, was found transferred to the bacteria in the gut contents from the colostomy bags of human volunteers. (i-sis.org.uk)
So, only a correspondingly tiny fraction of the actual transfers would ever be detected, especially given the well-known tendency of transgenic constructs to fragment and rearrange. (i-sis.org.uk)
When in a transgenic transferred the gene, there are now generations of GMOS where you can enter the gene and change it directly with the so-called editing genetics. (semillasdevida.org.mx)
That is to say that GMOS are a generic name that includes the transgenic and other agencies, regardless of the technique of molecular biology and the origin of the portion of DNA that has been modified. (semillasdevida.org.mx)
Transgenic seeds are the product of the application of the developments of modern biotechnology, whose foundations were laid in the mid-TWENTIETH century, with the discoveries of molecular biology with regard to the molecular structure of DNA. (semillasdevida.org.mx)
These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)

That is, "foreign" genes can be purposefully introduced into the germ line (reproductive) cells of the patient by means of such vectors, the reason being to genetically "enhance" or engineer the future progeny of the patient. (lifeissues.net)
Vectors used to transfer genes are divided into two Viral and Non-viral categories. (genetherapynet.com)
This platform is used to produce viral vectors for gene transfer, both in vivo and in vitro. (lu.se)

Microbial genetics applied to biotechnology principles and techniques of gene transfer and manipulation. (wikipedia.org)
BSc Microbiology has a strong practical basis, and you will learn a wide variety of techniques such as microbial genetics, biochemistry and propagation. (reading.ac.uk)
Welcome to the fascinating world of molecular biology , where biologists study the intricate molecular mechanisms of biological molecules that determine our traits through genomics and mendelian genetics. (freescience.info)
In this article series on molecular biology, we will dive deeper into the captivating realm of genomics, where biologists study the genome and explore the fascinating world of Mendelian genetics. (freescience.info)
For instance, in cancer treatment, molecular biology techniques have led to breakthroughs in genomics and genetics such as personalized medicine. (freescience.info)
Thanks to molecular biology techniques like DNA sequencing and gene expression analysis, biologists have made significant strides in understanding genetics and genomics. (freescience.info)
In the past 20 years the techniques of genetics and molecular biology have converged to provide both a remarkably detailed understanding of the genes that define the molecular composition of any organism and the ability to transfer genes from one species to another. (timeshighereducation.com)
Genetics in Sustainable Fisheries Management sets out to apply conservation and biotechnology to protect natural fish populations an wild fish gene pools, while addressing the issues concerning biosafety and sustainable management. (chipsbooks.com)

These studies have provided fundamental insights into UGT gene structure and regulation, isozyme substrate selectivity, and interindividual variability. (aspetjournals.org)
Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
Internationally renowned for his pioneering work in molecular cardiology, Dr. Williams has focused his work on the regulation of mitochondrial biogenesis using gene transfer and development biology techniques. (unthsc.edu)

To explore the concepts of genomics, molecular biologists employ cutting-edge techniques such as PCR (polymerase chain reaction), cloning, sequencing, and genetic engineering. (freescience.info)
In collaboration with the CDC Office of Advanced Molecular Detection , we recently launched a new, five-year initiative to strengthen public health capacity by introducing elements of human genomics into both public health surveillance and applied research. (cdc.gov)

The "foreign" gene that is natural or itself genetically engineered is inserted into the genome of the vector. (lifeissues.net)
Molecular biology techniques are instrumental in designing safe and effective gene delivery systems for the manipulation of DNA and genome. (freescience.info)
A newer technique, called genome editing (an example of which is CRISPR-Cas9), uses a different approach to correct genetic differences. (medlineplus.gov)
Instead of introducing new genetic material into cells, genome editing introduces molecular tools to change the existing DNA in the cell. (medlineplus.gov)
Genome editing is a promising technique also under study that doctors hope to use soon to treat disorders in people. (medlineplus.gov)
The striking progress in genome science and gene technology has led to numerous discoveries and the rapid development of new technologies in the life sciences. (jnss.org)
If it is desired that the transfected gene actually remains in the genome of the cell and its daughter cells, a stable transfection must occur. (newworldencyclopedia.org)

Bacteriophage transduction is the major mechanism of horizontal gene transfer (HGT) among many bacteria. (bvsalud.org)
They studied horizontal gene transfer. (universetoday.com)
By examining thousands of species of modern bacteria, they were able to find at least 34 examples of horizontal gene transfer in cyanobacteria's history. (universetoday.com)
I-SIS submitted written questions to the upcoming 'Open Meeting' of the Advisory Committee for Novel Food Products (ACNFP) in which horizontal gene transfer is to be discussed. (i-sis.org.uk)
Since then, we have continued to warn our regulators that the CaMV 35S promoter will be extra prone to spread by horizontal gene transfer and recombination [7-9]. (i-sis.org.uk)

Molecular biology, a branch of science, has revolutionized the field of medicine by providing insights into the intricate mechanisms underlying diseases at a molecular level. (freescience.info)
Studies on the mechanisms that regulate UGT genes, in a temporal and tissue-specific manner, should contribute significantly to understanding the basis for these differences. (aspetjournals.org)
Elevated excitability and its ac- and the molecular mechanisms of memory. (lu.se)

In Staphylococcus aureus, the phage-mediated acquisition of mobile genetic elements (MGEs) that encode virulence and antibiotic resistance genes largely contribute to its evolutionary adaptation and genetic plasticity. (bvsalud.org)
Antibiotic resistance genes spreading to pathogenic bacteria. (i-sis.org.uk)
These are genes added to the plasmid that provide an easily identifiable trait, such as resistance to an antibiotic or fluorescence. (jax.org)

Here we describe our studies on the genetic organization of phages infecting Staphylococcus aureus, a pathogen of human importance, by using an assembly of tools for gene annotation, identification of expression components, and phylogeny analysis. (bvsalud.org)
Studies on rodent UGT genes have demonstrated that the transcription factors hepatocyte nuclear factor 1 (HNF1) and CAAT-enhancer binding protein are important positive regulators of UGT expression in the liver (Hansen et al. (aspetjournals.org)
Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
In molecular biology, the term transformation has been used in the related sense to refer to the genetic alteration of a cell resulting from the uptake, genomic incorporation, and expression of foreign genetic material ( DNA ). (newworldencyclopedia.org)
Early visualized detection of oncogene expression provides novel early treatment of tumors through the antisense oligodeoxynucleotide (ASODN), which regulates gene expression by preventing genetic information from transferring to the protein. (molvis.org)
We are investigating how female lymphocytes maintain X-chromosome Inactivation, which is an epigenetic process responsible for equalizing gene expression between sexes. (upenn.edu)

Plasmid-mediated gene complexes confer high-level resistance to vancomycin and are often used as targets for molecular detection of VRE. (uspharmacist.com)
Molecular detection of Leishmania spp. (who.int)
The enhancement in detection of rare, treatable diseases in newborns will be accomplished through 1) the development of requirements to meet NBS program needs in OAMD's existing LIMS Lite application for connecting public health laboratory sequencing data with CDC, 2) NBS-specific bioinformatics pipeline/s construction, modifications, and validation and connection to the existing ED3N platform for transfer of variant calls and variant interpretation within ED3N. (cdc.gov)

This laboratory and lecture course is designed to provide neuroscientists at all levels with a conceptual and practical understanding of several of the most advanced techniques in molecular neuroscience. (cshl.edu)
Frontiers in Molecular Neuroscience. (lu.se)

Utilizing modern molecular biologic techniques such as the polymerase chain reaction, gene cloning and gene transfer, Dr. Wayne Grody's laboratory is involved in the elucidation, diagnosis and ultimately the treatment of single-gene defects at the molecular level. (uclahealth.org)
Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene internal transcribed spacer-1 (ITS1) was used. (who.int)
Several techniques based on the polymerase chain reaction (PCR) to facilitate the characterization and phylogenetic analysis of variola virus isolates were described. (who.int)

For the Harvard team, the target of the tool is a gene called CCR5 , which encodes a protein that adorns the surface of the immune sentinels targeted by the virus. (vilcek.org)
Mutations in ABCA12 , a gene that encodes adenosine triphosphate (ATP)-binding cassette transporter (ABC), subfamily A, member 12, in chromosome region 2q35, underlie this disorder. (medscape.com)

Simply put, the split markers involve dividing the marker genes at specific sites and fusing them to protein splicing elements. (jax.org)

This occurs when genes jump from one species to another, after being eaten, for example (as opposed to vertical gene transfer, which occurs from parent to child). (universetoday.com)

By unraveling the complex interactions between genes and their regulatory elements, researchers can identify disease-causing mutations more accurately. (freescience.info)

The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)
We form cationic liposomes as gene transfer reagents, and we can easily introduce genes into cells, including HilyMax, nucleic acid extraction reagents, nucleic acid staining reagents, electrophoresis reagents, and DNase and RNase that can be used for molecular biology applications. (dojindo.com)
Cooperation between staff at the two collaborating centres has begun, in order to ensure that any future work on viral characterization, including the transfer of reagents, is adequately coordinated. (who.int)

The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)

Such studies should also aid in the design of molecular probes to assess the capacity of individuals to metabolize specific drugs and toxins, before exposure to these agents. (aspetjournals.org)
The molecular structure of probes is easily visualized under AFM. (molvis.org)
AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes. (molvis.org)

For most applications of transfection, it is sufficient if the transfected gene is only transiently expressed. (newworldencyclopedia.org)

For him the genes define the essence of life and the organism is just a survival machine built by genes for their own perpetuation. (timeshighereducation.com)
What is wrong with these statements is that they define scientific positions that need to be backed up by models that demonstrate precisely how a knowledge of genes in the developing organism will lead to an understanding of the three-dimensional form of the human heart or limb or eye, the arrangement of leaves on a plant and the organs of the flower, or the wings of a fruit fly. (timeshighereducation.com)
Transgenes are genes transferred from one organism to another, and they are important for many research areas. (jax.org)

We used a replication defective recombinant retrovirus vector GINaTK (bearing HSV-TK gene) to make packaging cell PA317 produce progeny virions. (wjgnet.com)
In harlequin ichthyosis, the ABCA12 -mediated transfer of lipid to lamellar granules is defective. (medscape.com)

Molecular biology techniques, such as DNA sequencing and genotyping, have facilitated the identification of genetic variations that impact drug metabolism and efficacy. (freescience.info)
Molecular biology techniques have been used in a wide range of fields to elucidate the functions of living organisms at the molecular level. (dojindo.com)

This isolated population has limited gene flow with other bird populations and experiences genetic drift as it evolves independently. (sigmachihq.org)

Molecular biology, a branch of science that combines biology and chemistry, is crucial for advancements in medicine, biotechnology, agriculture, and our understanding of evolution and biodiversity. (freescience.info)
During the second half of the TWENTIETH century developed other techniques of genetic engineering to the manipulation of the genetic material of living organisms, increasingly moving away from traditional biotechnology executed by the peasant communities from the origins of agriculture in the Neolithic period. (semillasdevida.org.mx)

This task can be accomplished by replacing tumor suppressor genes that have been lost or mutated, selectively inserting genes that produce cytotoxic substances, or modifying the immune system to destroy the tumor cells. (medscape.com)
We then transferred the HSV-TK gene to target cells SW1990 using these progeny virions, and treated these gene-modified tumor cells with GCV to study the sensitivity of the cells to GCV and their bystander effects by routine MTT-method. (wjgnet.com)

The UGT2 family, in contrast, is encoded by separate genes clustered on chromosome 4q13 and consists of the UGT2A and UGT2B subfamilies. (aspetjournals.org)
The underlying genetic abnormality in harlequin ichthyosis is a mutation in the lipid-transporter gene ABCA12 on chromosome 2. (medscape.com)

2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)

[ 3 ] In an attempt to improve outcomes in HNSCC, most research in the field is now focusing on the molecular biology of HNSCC in an attempt to target select pathways involved in carcinogenesis. (medscape.com)
Over a career spanning nearly three decades and a wide array of themes in immunology, Littman has unraveled intricately braided molecular pathways that control the identity of immune cells, the specificity of immune responses, the ability of immune cells to turn against their hosts, and the interplay between immune cells and the human body's microbial denizens. (vilcek.org)

Molecular biology aids in drug discovery by identifying potential therapeutic targets and evaluating their efficacy in DNA. (freescience.info)

Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields. (bvsalud.org)
Using new molecular techniques, they transferred late-blight resistance genes into the popular East African potato variety Victoria. (monitor.co.ug)
For example, a gene (DNA sequence that codes for a characteristic) of a bacterium (such as resistance to insects) introduced into a plant. (semillasdevida.org.mx)
Although some species are inherently resistant to vancomycin, they are far less common than species that acquire resistance through transfer of genetic material. (uspharmacist.com)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
The National Research Council summed up the situation: "no conceptual distinction exists between generic modification of plants and microorganisms by classical methods or by molecular techniques that modify DNA and transfer genes. (theatheistconservative.com)
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. (cshlpress.com)
Research at the ASMG lab focuses primarily on the gut microbial composition of ruminants utilizing advanced molecular methodologies. (upenn.edu)
It reflects a neuroadaptation that is the likelihood of drug-seeking behav- mediated gene transfer as a tool for neuropsychi- .nature the result of a powerful psychological iors, whereas administration of small atric research. (lu.se)
Leveraging existing technology and stored specimens, HLA genes and variants of immune response genes (IL-10, TNFA, IL1A, IL12B, and CD80) are to be sequenced and genotyped, respectively, establishing a broader set of Hemophilia Inhibitor Research Study (HIRS) enrollees to evaluate existing inhibitor risk prediction models and to investigate the feasibility of developing better models using curated variants in a diverse patient population. (cdc.gov)

Here, we describe optimized protocols for generalized transduction, applicable for the transfer of plasmid or chromosomal deoxyribonucleic acid (DNA) from donor to recipient S. aureus strains. (bvsalud.org)

Disease-associated genes spreading and recombining to create new viruses and bacteria that cause diseases. (i-sis.org.uk)

The informal and interactive evening lectures will be given by leading molecular neuroscientists and will serve to illustrate the ways in which the various experimental approaches have been used to advance specific areas of neurobiology. (cshl.edu)

Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)

Neither technique is highly efficient, however, making it necessary to select the cells that actually receive a functional version of the desirable gene and discard the rest. (jax.org)

As our understanding of the molecular biology of HNSCC continues to develop, we can target the specific components of cancer cells that are not found in normal cells. (medscape.com)
Hopefully the "diseased" gene in the patient will be "corrected" by, or at least supplement, these disease-causing cells in the patient. (lifeissues.net)
Introduce a new gene into cells to help fight a disease. (medlineplus.gov)
Studies of a pckA-lacZ gene fusion indicated that when cells were grown in minimal media with various carbon sources, such as succinate, malate, pyruvate, lactate, or ethanol, under both anaerobic light and aerobic dark conditions, the pckA gene was induced in log phase, irrespective of the carbon source. (asm.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
The term transformation often is preferred to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells such as fungi , algae , and plants . (newworldencyclopedia.org)
For example, it's possible to introduce human versions of genes into mouse cells or even live mice to study their effect on the cells' or animals' phenotypes, or traits. (jax.org)
HPC-A collection, processing, storage and transplant cGMP manufacturing of cell and gene modified human cells for clinical use. (genetherapynet.com)
Lastly, we evaluated the strategy of knocking out a-syn as a means to protect the cells from transfer of pathology upon grafting. (lu.se)

Only those strains with tfdA sequences highly similar to the tfdA sequence of strain JMP134 (tfdA class I) transferred all the 2,4-D genes and conferred the 2,4-D degradation phenotype to a Burkholderia cepacia recipient. (nih.gov)

As director of the UCLA Medical Center's Diagnostic Molecular Pathology Laboratory, Grody can offer his trainees exposure to the latest clinical applications of molecular biology in the diagnosis of a wide range of genetic, neoplastic and infectious diseases as well as DNA fingerprinting. (uclahealth.org)
VRE colony counts are similar in the stools of colonized and infected patients, so diagnosis of infection requires additional clinical assessment and culture techniques, as discussed below. (uspharmacist.com)

The development of molecular imaging at the end of the 20th century has provided a novel visualization for early and non-invasive diagnoses of diseases. (molvis.org)

The group used molecular techniques to transfer the l-carnitine destruction genes into a strain of E. coli that is considered safe for humans, but didn't originally have those genes. (illinois.edu)

Through DNA and genetic engineering techniques, scientists can introduce desirable traits into crops more efficiently than traditional breeding methods. (freescience.info)

Handbook of molecular microbial ecology II : metagenomics in different habitats. (wikipedia.org)

One reason that inefficient organic agriculture uses more water is that it excludes the cultivation of crop varieties crafted with molecular genetic-modification techniques-so-called GMOs-that can be made to withstand droughts and to be irrigable with brackish water. (city-journal.org)

Molecular biology , a field studied by biologists, focuses on the study of biological molecules and their interactions at the molecular level. (freescience.info)

When the genetically engineered vector replicates inside the human host, this "foreign" gene will also be replicated along with the vector's original genes -- and thus be transferred to the human host. (lifeissues.net)
The use of the technique of nuclear transfer for reproduction of human beings is surrounded by strong ethical concerns and controversies and is considered a threat to human dignity. (who.int)
This technique is surrounded by strong ethical concerns and is considered a threat to human dignity. (who.int)

During studentships you will typically work on a set project and benefit from valuable training in techniques, experimental design and the interpretation of data. (reading.ac.uk)

The pckA gene, encoding the gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK), was cloned by PCR amplification from the purple nonsulfur bacterium Rhodopseudomonas palustris No. 7. (asm.org)

Another method involves what is called molecular clock dating, which looks at the fossil record to measure the rate of genetic changes over time. (universetoday.com)

2. An assortment of molecular markers are being used for genotype identification and for the development of genetic linkage maps in Vitis . (cornell.edu)

With the latter we want to make a detailed analysis of all transfers of energy that affect the functions of production, consumption, and recovery in the social system. (ac.be)
Yet we are constantly being told that molecular analysis will reveal all. (timeshighereducation.com)

That means that the "foreign" gene will be accidentally passed down through successive generations of that patient's progeny. (lifeissues.net)

Serological techniques are the most specific methods for identifying rhizobia based on natural marker characteristics. (benthamscience.com)

Anatomy2

Organisms3

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Psychiatry and Psychology1

Phenomena and Processes11

Information Science2

Health Care1

Proteins6

Bacterial2

Structure and tissue-specific regulation2

Therapies8

Genetically modified3

Therapy34

Transgenic10

Vectors3

Genetics8

Regulation3

Genomics2

Genome7

Horizontal gene tra5

Mechanisms3

Antibiotic3

Expression6

Detection3

Neuroscience2

Polymerase3

Encodes2

Protein1

Species to another1

Researchers1

Reagents3

Characterization1

Probes3

Transfection1

Organism3

Defective2

Biology techniques2

Populations1

Biotechnology2

Tumor2

Chromosome2

Cloning2

Pathways2

Targets1

Resistance4

Research7

Plasmid1

Viruses1

Approaches1

Bacteriophage1

Functional1

Cells9

Sequences1

Clinical2

Diseases1

Coli1

Desirable1

Microbial1

Genetic-modification1

Molecules1

Human3

Experimental1

Rhodopseudomonas1

Involves1

Markers1

Analysis2

Progeny1

Specific1