Super-resolution microscopyFluorescence microscopesSpinning-disc confocalPoint scanningConventionalIllumination microscopyMulti-photon micFixed and living cells andFluorescentImagingLasersCryo-Electron MAdvanced microscopy techniquesUprightStereo microscopesSpectralDiffraction limitLightRamanBiomedicalInstrumentationTransmission electron mBiologicalAdvancesResearchersAdaptive opticsDeconvolutionSpecimenHigh numerical apertureSensitivityMultipointCompound microscopeLaserImagesSTEDSheppardPiezoelectricResearch microscopeScanningOptical fibreSingle moleculeLensesLocalisationSystemFluorescentlyPhase contrastDetectorCapabilityResolutionContrastMagnificationZEISS
Super-resolution microscopy3
- Super-resolution microscopy overcomes this diffraction limit and allows scientists to observe and study subcellular or even molecular processes in greater detail. (edu.hk)
- This is particularly useful when one needs to perform multi-colour imaging, and when not all fluorescent labels are compatible with super-resolution microscopy. (edu.hk)
- Our data includes confocal microscopy, multi-photon microscopy, super-resolution microscopy, and other advanced microscopy methods (namely structured illumination microscopy, light sheet fluorescence microscopy, and deconvolution fluorescence microscopy). (bioinfoinc.com)
Fluorescence microscopes4
- Light microscopy instrumentation includes the following devices: two routine fluorescence microscopes and a confocal microscope. (edu.pl)
- Adding Microvolution to the Etaluma system will enable users to further improve resolution in the LS line of inverted fluorescence microscopes," offered Marc Bruce, PhD, CEO of Microvolution. (etaluma.com)
- We strive to make the best inverted fluorescence microscopes to meet your needs at the most affordable prices. (etaluma.com)
- Hell worked with fluorescence microscopes. (abberior.rocks)
Spinning-disc confocal1
- An example of science driving the technical advances is spinning-disc confocal microscopy. (photonics.com)
Point scanning2
- The widefield confocal technology of Smartproof 5 offers greater throughput compared to classical point scanning confocal microscopes. (zeiss.com)
- Laser point scanning confocal microscopy (LCSM) [1] gave robust sectioning to produce excellent 3D images, but was typically slow and phototoxic. (biologists.com)
Conventional11
- Conventional, widefield microscopy is generally unsuitable for imaging thick tissue because the images are corrupted by a blurred, out-of-focus background signal. (wikipedia.org)
- Single fibre endomicroscopes have similar resolution of a conventional confocal microscope. (wikipedia.org)
- Part I: Conventional microscopy. (microscopyu.com)
- The best resolution of conventional light microscopy is limited to ~200 nm due to the diffraction of light. (edu.hk)
- Real-time images produced by the new device (black and white) show similar detail to conventional pathology laboratory microscopes and imaging techniques (color). (iflscience.com)
- Light scattered in multiple directions by the target tissue produces erroneous "background noise" for conventional microscopes. (iflscience.com)
- One widefield microscope is a conventional Olympus IX-70 inverted fluorescence microscope. (edu.pl)
- Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical sections from thick specimens. (olympusconfocal.com)
- There has been a tremendous explosion in the popularity of confocal microscopy in recent years, due in part to the relative ease with which extremely high-quality images can be obtained from specimens prepared for conventional fluorescence microscopy, and the growing number of applications in cell biology that rely on imaging both fixed and living cells and tissues. (olympusconfocal.com)
- In a conventional widefield optical epi-fluorescence microscope, secondary fluorescence emitted by the specimen often occurs through the excited volume and obscures resolution of features that lie in the objective focal plane. (olympusconfocal.com)
- Even though resolution is somewhat enhanced with confocal microscopy over conventional widefield techniques, it is still considerably less than that of the transmission electron microscope. (olympusconfocal.com)
Illumination microscopy4
- Total internal reflection fluorescence (TIRF) [6] microscopy could give exquisite z resolution, but over a very limited range, and the structured illumination microscopy (SIM) [7] methods of the time were limited to shallow depths at moderate frame rates. (biologists.com)
- It was in early 2014 that I became aware of Andrew York 's work on instant structured illumination microscopy (iSIM) [8] . (biologists.com)
- I have omitted thus far one of the simplest and most powerful concepts in biological imaging: selective plane illumination microscopy (SPIM) [10] or light-sheet fluorescence microscopy (LSFM) [11] . (biologists.com)
- In structured illumination microscopy (SIM), the sample is illuminated with a series of highly structured excitation light ( e.g ., a pattern of parallel lines). (edu.hk)
Multi-photon mic2
- It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use. (wikipedia.org)
- Optical coherence tomography and multi-photon microscopy have both been demonstrated endoscopically. (wikipedia.org)
Fixed and living cells and1
- The Laboratory of Fluorescence Microscopy supplies high-quality microscopic imaging of fixed and living cells and tissues. (edu.pl)
Fluorescent7
- The problem is that the confocal volume sensibly depends on numerous parameters of the optical set-up, refractive index mismatch of the sample solution and the objective's immersion medium, coverslide thickness variations and especially the dependence on optical saturation of the fluorescent dye, which can occur at even very low excitation powers. (picoquant.com)
- The rapid expansion in biomedical research using live-cell imaging techniques over the past several years has been fueled by a combination of events that include dramatic advances in spinning disk confocal microscopy instrumentation coupled with the introduction of novel ultra-sensitive detectors and continued improvements in the performance of genetically-encoded fluorescent proteins. (fsu.edu)
- Furthermore, the fixed spectral characteristics of interference filters do not allow optimization for different fluorophore combinations, nor for adaptation to new fluorescent dyes, limiting the versatility of both the excitation and detection functions of the microscope. (olympusconfocal.com)
- Fluorescence imaging is often used in a light microscopy setting and revolved around the use of either autofluorescent properties of the sample or fluorescent marker molecules. (helmholtz-imaging.de)
- Yonghong Shao, "Multi-Laser Scanning Confocal Fluorescent Endoscopy Scheme for Subcellular Imaging (Invited)," Progress In Electromagnetics Research , Vol. 169, 17-23, 2020. (jpier.org)
- Tony Wilson, Brad Amos, and John White nurtured the concept and later (during the late 1980s) demonstrated the utility of confocal imaging in the examination of fluorescent biological specimens. (olympusconfocal.com)
- Confocal laser scanning microscopy is mainly used in biomedical sciences for high-resolution analysis of cell and tissue structures labeled with fluorescent markers. (lu.se)
Imaging28
- The addition of micro-optics at the distal tip of the bundle allows for magnification and hence higher resolution imaging, but at the cost of reducing the field-of-view. (wikipedia.org)
- Widefield endomicroscopes (i.e. non-depth sectioning microscopes) have been developed for select applications, including the imaging of cells ex vivo. (wikipedia.org)
- This symbiotic relationship is evident at Harvard Medical School's Nikon Imaging Center in Boston, where several microscope manufacturers have placed state-of-the-art equipment on perpetual loan. (photonics.com)
- The MicroTime 200 time-resolved fluorescence microscope system is a powerful instrument capable of Fluorescence Lifetime Imaging (FLIM) with single molecule detection sensitivity. (picoquant.com)
- We demonstrate the modalities of bright- and dark-field imaging and scanning fluorescence microscopy at acquisition rates that allow observation of dynamic processes such as Brownian motion of mesoscopic particles. (nature.com)
- Separately, we are seeing a burgeoning need for imaging devices that offer observation in areas with typically very limited access and thus avoid the use of standard microscope platforms. (nature.com)
- Spinning Disk Confocal Microscopy - Spinning disk microscopy has advanced significantly in the past decade and now represents one of the optimum solutions for both routine and high-performance live-cell imaging applications. (fsu.edu)
- Instruments equipped for spectral imaging are becoming increasingly popular and many confocal microscopes now offer this capability. (fsu.edu)
- This course will provide the participants with an overview about the basics of microscopy (light and optics, contrast techniques and imaging). (conference-service.com)
- The confocal part will include multicolor, 3D and time lapse imaging as well as spectral imaging, photobleaching (FRAP, FLIP, FLAP) and photoactivation techniques. (conference-service.com)
- I fortuitously stumbled into the field of microscopy around 2013, just in time to enjoy the hype of the 2014 Chemistry Nobel Prize for super-resolved fluorescence imaging. (biologists.com)
- In addition to serving as a standalone confocal or super-resolution microscope, the Nikon N-SIM/N-STORM/A1 system also allows researchers to perform correlative confocal-super-resolution imaging. (edu.hk)
- It is an imaging technique similar to fluorescence microscopy which is able to detect absorbing nanoparticles (NPs) of sizes down to 1nm and even single absorbing molecules. (jimdofree.com)
- We are recruiting a Postdoctoral Researcher to develop the next generation of light-sheet microscopes for biomedical imaging. (jobs.ac.uk)
- The central aim of this research is to overcome the imaging depth limit in biological microscopy. (jobs.ac.uk)
- This multi-disciplinary project fuses computational imaging and adaptive optics in microscopy , as well as the further development of existing systems with a range of modalities such as light-sheet microscopy, structured illumination, phase contrast, and confocal microscopy. (jobs.ac.uk)
- A scanning helium ion microscope (SHIM, HeIM or HIM) is an imaging technology based on a scanning helium ion beam. (helmholtz-imaging.de)
- Fluorescence confocal laser scanning endomicroscopy is a novel tool combining confocal microscopy and endoscopy for in-vivo subcellular structure imaging with comparable resolution as the traditional microscope. (jpier.org)
- With Celldiscoverer 7, you combine the ease-of-use of an automated high-end imaging system with the image quality and flexibility of a classic inverted research microscope. (zeiss.co.uk)
- ZEISS Celldiscoverer 7 is an automated live cell imaging system designed to make complex microscopy simple enabling high-throughput applications, but what about the data? (zeiss.co.uk)
- The microscopy systems are set up for imaging stained cells on glass slides or in chambered cover glass. (edu.pl)
- ZEISS FIB-SEMs , Focused Ion Beam Scanning Electron Microscopes, combine 3D imaging and analysis performance of the Gemini electron beam column with the ability of a FIB for material processing and sample preparation on a nanoscopic scale. (antisel.gr)
- Likewise, widefield fluorescence imaging of whole rabbit muscle fibers stained with fluorescein produce blurred images (Figure 1(b)) lacking in detail, while the same specimen field (Figure 1(e)) reveals a highly striated topography in confocal microscopy. (olympusconfocal.com)
- July 25, 2017 - (Carlsbad, CA) Today Etaluma and Microvolution announced a strategic partnership that combines the power of imaging by Etaluma's next generation inverted fluorescence LS Microscopes (Lumascopes) and Microvolution's powerful GPU-based deconvolution algorithms. (etaluma.com)
- Etaluma's high resolution fluorescence LS Microscopes offer a simplified approach to live cell imaging specifically and research microscopy in general. (etaluma.com)
- Providing true next-gen microscopy, the compact, inverted design allow imaging in a wide range of labware and settings, including live cell imaging in incubators, hoods, and hypoxia chambers. (etaluma.com)
- Etaluma newest product, the LS720, automates the LS620 3-color optical platform with an automated XY stage and autofocus, combining high resolution cell imaging with high content microscopy at an affordable price. (etaluma.com)
- Imaging silicon atoms with terahertz-driven scanning tunnelling microscopy (TD-STM). (rhk-tech.com)
Lasers3
- Introduction of the acousto-optic tunable filter to confocal systems overcomes most of the filter wheel disadvantages by enabling rapid simultaneous electronic tuning and intensity control of multiple laser lines from several lasers. (olympusconfocal.com)
- Heidelberg Engineering's growing product portfolio combines these core technologies: confocal microscopy, scanning lasers and optics, optical coherence tomography (OCT), real-time image processing and analytics, multimodal image management solutions (PACS), electronic medical records (EMR) and large-scale data analysis. (heidelbergengineering.com)
- This method can be used in any commercially available inverted confocal microscope, doesn't require expensive lasers or optics, exhibits minimal organ perturbation, photo bleaching, or phototoxicity , and, hence, it enables the study of tissue -resident macrophages in situ and in vivo under steady state and inflammation . (bvsalud.org)
Cryo-Electron M1
- In 2017, the Nobel Prize in Chemistry was awarded for cryo-electron microscopy, furthering demonstrating that the development of new ways to "see" biological processes in high resolution is still incredibly important to life sciences research. (bioinfoinc.com)
Advanced microscopy techniques1
- While learning about advanced microscopy techniques, it became apparent that gains in system performance in one area were often traded for losses somewhere else. (biologists.com)
Upright3
- It perfectly combines upright optical, inverted optical, and atomic force microscopies, and unleash all the power of both techniques providing instrument adjustment and measurement automation, high resolution and integration flexibility. (horiba.com)
- The TRIOS platform combines upright and transmission configurations which allows to study transparent as well as non-transparent samples with optical, Raman, Photoluminescence and scanning probe microscopy techniques. (horiba.com)
- One widefield microscope is an Eclipse E800 upright fluorescence microscope. (edu.pl)
Stereo microscopes3
- Stereo microscopes are also quite common, though not as prevalent as compound microscopes. (opticsmag.com)
- While compound and stereo microscopes are by far the most common types of these tools, there are quite a few other varieties of microscopes that have their own specific uses. (opticsmag.com)
- Stereo Microscopes generate three-dimensional, laterally precise images for your microscope applications. (antisel.gr)
Spectral3
- The integration of optoelectronic technology into confocal microscopy has provided a significant enhancement in the versatility of spectral control for a wide variety of fluorescence investigations. (olympusconfocal.com)
- In the traditional fluorescence microscope configuration, including many confocal systems, spectral filtering of both excitation and emission light is accomplished utilizing thin-film interference filters. (olympusconfocal.com)
- Although the function of the acousto-optic tunable filter is similar to that of a diffraction grating, its behavior differs in that diffraction occurs over an extended volume of the crystal rather than at a planar surface, and only a limited band of spectral frequencies are affected. (olympusconfocal.com)
Diffraction limit2
- These tools help researchers to approach or break through the diffraction limit, which limits microscope resolution of traditional microscopes to a few hundred nanometers. (bioinfoinc.com)
- According to Abbe's diffraction limit, objects must be about 200 nm apart to be distinguishable under a light microscope. (abberior.rocks)
Light20
- Prior Scientific, for example, brings automated shutters and focus mechanisms to the lab, allowing ongoing light microscopy studies to be performed as simply and accurately as possible. (photonics.com)
- Here we present a novel approach for this field by utilizing disordered light within a standard multimode optical fibre for lensless microscopy and optical mode conversion. (nature.com)
- These may be useful for future fibre-based implementations of super-resolution or light-sheet microscopy. (nature.com)
- Focusing of light through a stratified medium: A practical approach for computing microscope point spread functions. (microscopyu.com)
- Light Sources for Optical Microscopy - The performance of the various illumination sources available for optical microscopy depends on the emission characteristics and geometry of the source, as well as the focal length, magnification and numerical aperture of the collector lens system. (fsu.edu)
- If you're using the most common type of microscope, the compound microscope, there will be light passing directly through the specimen, allowing you to see what's going on inside of it. (opticsmag.com)
- With a stereo microscope, the light doesn't pass through the object you're viewing. (opticsmag.com)
- Since the light must pass through the specimen on such a microscope, the slide is completely clear, enabling the light to reach the specimen. (opticsmag.com)
- Light microscopy is the most widely used form of microscopy for life science research. (conference-service.com)
- Most light-sheet instruments violate this boundary by requiring two or more orthogonal lenses (for illumination and collection), and whilst some users can (and will) re-engineer their samples for a light-sheet microscope, most will not . (biologists.com)
- Users are given the opportunity to perform their experiments independently or receive support from facility staff to generate and interpret microscopic data based on light microscopy. (edu.pl)
- The Nikon C1 confocal laser scanning microscope is built on a Nikon TE2000 inverted stand and fitted with a laser box that includes four laser lines, a confocal head with substitutive first dichroic mirrors, a 3 PMTs for three fluorescence channels, and a detector for transmitted light, coupled with controlling computers with EZ-C1 software for viewing and capturing microscopic data. (edu.pl)
- Polarized Light Microscopy for Research Tasks. (antisel.gr)
- Recent progress in sub-diffraction light microscopy has allowed researchers to look into cells with an unrivalled level of detail and specificity. (unige.ch)
- The basic key to the confocal approach is the use of spatial filtering techniques to eliminate out-of-focus light or glare in specimens whose thickness exceeds the immediate plane of focus. (olympusconfocal.com)
- than TEM or light microscopy. (freezingblue.com)
- For over a century, we stood at the edge of microscope resolution and cursed the inexorable blur of diffracted light. (abberior.rocks)
- No matter how sophisticated the instrument, a point of light viewed through a microscope becomes a smeared blob because light waves passing through the aperture of an objective diffract. (abberior.rocks)
- Rather than trying to change the optics of a microscope, Stefan Hell exploited a loophole to outsmart the arrogance of light and blast through the resolution barrier. (abberior.rocks)
- The fluorescence microscope targets light onto the probes, sending them into a state of excitation, and then detects the photons they emit upon returning to their ground state. (abberior.rocks)
Raman4
- TRIOS can be easily combined to our turn-key Raman/PL systems for co-localized AFM-Raman/PL measurements, Scanning Near-Field Optical Microscopies (SNOM), and Tip-Enhanced Optical Spectroscopies (TERS: Tip-Enhanced Raman Spectroscopy and TEPL: Tip-Enhanced Photoluminescence). (horiba.com)
- The Raman microscope is a laser-based microscopic device used to perform Raman spectroscopy. (helmholtz-imaging.de)
- The term MOLE (molecular optics laser examiner) is used to refer to the Raman-based microprobe. (helmholtz-imaging.de)
- The confocal Raman instrument used in this experiment is based on an inverted optical microscope equipped with an x,y-scan stage. (rhk-tech.com)
Biomedical1
- A study in the journal Biomedical Optics Express describes a new handheld microscope that has the ability to image various tissues with as much detail as the best laboratory equivalents. (iflscience.com)
Instrumentation1
- The Laboratory of Fluorescence Microscopy provides basic methods and instrumentation background for fluorescence microscopy techniques. (edu.pl)
Transmission electron m1
- A scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). (helmholtz-imaging.de)
Biological1
- When it became available for biological microscopes, researchers let manufacturers know what they needed so as to use this new science consistently, and manufacturers responded with special optics, lighting, highly accurate focus capability, sensitive cameras and software. (photonics.com)
Advances6
- Thanks to recent advances in microscopes and support tools - from cameras to positioning systems and shutters - that make up the setup, image acquisition and data collection are becoming faster and more accurate. (photonics.com)
- As microscopy advances, the demands for enhancements in support technologies increase. (photonics.com)
- And as technical advances make their way into commercial products, they fuel further advances in microscopy. (photonics.com)
- Because of microscopes, massive advances have been made in many areas, including medical and scientific fields. (opticsmag.com)
- In fact, confocal technology is proving to be one of the most important advances ever achieved in optical microscopy. (olympusconfocal.com)
- During the late 1970s and the 1980s, advances in computer and laser technology, coupled to new algorithms for digital manipulation of images, led to a growing interest in confocal microscopy. (olympusconfocal.com)
Researchers6
- Plug-and-play microscopy is a fairly new concept that has some researchers shaking their heads. (photonics.com)
- Rounding out this series of features is a look at adaptive optics, which was once primarily a tool for astronomers but is now making its way into microscopy, where researchers use it to counter aberrations in their systems. (photonics.com)
- In the same field-of-view (FOV), researchers can choose to use confocal for certain fluorescence channels, and super-resolution for the remaining ones. (edu.hk)
- Alternatively, researchers can choose to use confocal to scan a larger FOV to provide an overview of the sample, and use super-resolution to image a selected area of interest to examine the area in greater detail. (edu.hk)
- Researchers at the University of Washington (UW) are currently engineering a miniature, powerful microscope that could solve this problem. (iflscience.com)
- As both life science and materials science researchers probe ever further into the realm of the nanoscale, greater resolution and capabilities from fluorescence microscopy are enabling new areas of investigation. (bioinfoinc.com)
Adaptive optics1
- Scientists at the University of Strathclyde's Institute of Photonics in Glasgow, UK, have done extensive research into adaptive optics in microscopy, and those results are reported here, along with a glimpse of what might be available commercially in the near future. (photonics.com)
Deconvolution1
- Upon launching the first GPU accelerated deconvolution algorithm in 2014, Microvolution jump-started the revolution in deconvolution microscopy. (etaluma.com)
Specimen5
- Modern compound microscopes are designed to provide a magnified two-dimensional image that can be focused axially in successive focal planes, thus enabling a thorough examination of specimen fine structural detail in both two and three dimensions. (microscopyu.com)
- Together, the optical and mechanical components of the microscope, including the mounted specimen on a glass micro slide and coverslip, form an optical train with a central axis that traverses the microscope base and stand. (microscopyu.com)
- The slide is a small piece of glass upon which you will prepare your specimen for viewing through a compound microscope. (opticsmag.com)
- Your specimen is the item that you're viewing with the microscope. (opticsmag.com)
- When using a compound microscope, the specimen will be prepared on a glass slide. (opticsmag.com)
High numerical aperture1
Sensitivity1
- Etaluma's fluorescence LS microscopes (Lumascopes) constitute a dramatic new concept in solid-state optics with high sensitivity, maximum resolution (near diffraction-limited), and zero pixel shift. (etaluma.com)
Multipoint1
- This method elegantly combined multipoint confocal scanning with SIM in an all-optical 'instant' implementation, where images would arrive at the camera chip with a root(2) boost in resolution (a total 2-fold with post-processing). (biologists.com)
Compound microscope2
- Using a compound microscope, you can view living and dead specimens, even as small as individual cells. (opticsmag.com)
- They offer a three-dimensional image, instead of the two-dimensional image you get from a compound microscope. (opticsmag.com)
Laser12
- Endomicroscopes achieve optical sectioning (removal of the background intensity) using the confocal principle - each image frame is assembled in a point-by-point fashion by scanning a laser spot rapidly over the tissue. (wikipedia.org)
- Optics in the probe focus the laser to a spot in the tissue, exciting fluorescence. (wikipedia.org)
- Looking for Custom Laser Optics? (edmundoptics.in)
- Visit Our New Online Laser Optics Manufacturing Hub. (edmundoptics.in)
- The acousto-optic tunable filter ( AOTF ) is an electro-optical device that functions as an electronically tunable excitation filter to simultaneously modulate the intensity and wavelength of multiple laser lines from one or more sources. (olympusconfocal.com)
- HRT3 RCM is a compact ophthalmic device that uses confocal scanning laser microscopy to provide high-resolution images of the cornea and external ocular structures, such as the conjunctiva or the limbus. (heidelbergengineering.com)
- In this paper, we propose a three-channel fluorescence confocal microscopy system based on fiber bundle and two excitation laser lines of 488nm and 650nm. (jpier.org)
- Presented in Figure 1 are a series of images that compare selected viewfields in traditional widefield and laser scanning confocal fluorescence microscopy. (olympusconfocal.com)
- When imaged with a laser scanning confocal microscope (Figure 1(d)), the medulla thick section reveals a significant degree of structural detail. (olympusconfocal.com)
- Continuing in this arena, Egger went on to develop the first mechanically scanned confocal laser microscope, and published the first recognizable images of cells in 1973. (olympusconfocal.com)
- Fortuitously, shortly after Minsky's patent had expired, practical laser scanning confocal microscope designs were translated into working instruments by several investigators. (olympusconfocal.com)
- High resolution is achieved by scanning the sample with a finely focussed laser beam, and exclusion of out-of-focus fluorescence by a confocal aperture. (lu.se)
Images6
- The final confocal images can then be overlaid with the corresponding super-resolution images in the same FOV. (edu.hk)
- A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons. (helmholtz-imaging.de)
- Scanning the cornea with a field of view of up to 400 x 400 μm, HRT3 RCM allows you to acquire unique en face images of corneal cells and structures, identify keratocytes subpopulations, and visualize details of the corneal subbasal nerve plexus. (heidelbergengineering.com)
- Microvolution's software offers a powerful solution for providing confocal-like images from widefield optics without the cost, complexity, and technical drawbacks of confocal microscopy," said Chris Shumate, PhD, CEO of Etaluma. (etaluma.com)
- The topography and near-field images were obtained with shear-force microscopy using a chemically etched gold tip attached to a quartz tuning fork. (rhk-tech.com)
- Scanning tunneling microscopy images of Aβ1−16-Cu2+ (a,b) and Aβ1−16 (c,d). (rhk-tech.com)
STED1
- Multi-photon(MP) [2] and stimulated emission depletion (STED) [3] microscopy could greatly improve depth and resolution limitations, but with the drawbacks of point scanners, often increasing sample damage. (biologists.com)
Sheppard1
- Dutch physicist G. Fred Brakenhoff developed a scanning confocal microscope in 1979, while almost simultaneously, Colin Sheppard contributed to the technique with a theory of image formation. (olympusconfocal.com)
Piezoelectric2
- It includes such application-specific modes as force mode, and nanolithographies, piezoelectric force microscopy (PFM), Kelvin Probe Microscopy and frequency modulation AFM (dynamic force microscopy with built-in PLL). (horiba.com)
- An acousto-optic tunable filter designed for microscopy typically consists of a tellurium dioxide or quartz anisotropic crystal to which a piezoelectric transducer is bonded. (olympusconfocal.com)
Research microscope1
- An inverted research microscope for investigation, development and analysis of materials. (antisel.gr)
Scanning4
- In table-top confocal microscopes the scanning is usually performed using bulky galvanometer or resonant scanning mirrors. (wikipedia.org)
- The Pentax Medical device was packaged into an endoscope, that used OptiScan's electromagnetic-controlled scanning of a single fibre to perform the confocal scanning at the distal tip of the device. (wikipedia.org)
- In addition to this, a technique called line scanning is employed by the microscope to speed up the image processing. (iflscience.com)
- Scanning tunneling microscopy of the Graph. (rhk-tech.com)
Optical fibre1
- Single fibre confocal endomicroscopes use the tip of an optical fibre as a spatial filter, enabling miniaturisation of the microscope. (wikipedia.org)
Single molecule2
- Single molecule localization microscopy (SMLM), like (PALM) [4] or (STORM) [5] , could yield an impressive boost in resolution, but at the expense of speed and complex sample preparation. (biologists.com)
- Stochastic optical reconstruction microscopy (STORM) is a widely-used single-molecule localisation microscopy technique. (edu.hk)
Lenses4
- After all, wasn't a microscope just some lenses to magnify small objects? (biologists.com)
- It is equipped with multiple objective lenses, DIC optics, and an epi-fluorescence attachment with changeable filter blocks. (edu.pl)
- It is equipped with multiple objective lenses, contrast phase optics, and an epi-fluorescence attachment with changeable filter blocks. (edu.pl)
- The TE2000 is equipped with multiple objective lenses, DIC optics, an epi-fluorescence attachment with changeable filter blocks, a motorized stage, and Nikon DS-U1 color camera. (edu.pl)
Localisation1
- In this demo we will use a super-resolution technique combining cutting-edge conical diffraction optics with advanced localisation algorithms. (unige.ch)
System6
- The versatile ZEISS Smartproof 5 widefield confocal microscope is your integrated system for surface analysis: fast, precise and repeatable. (zeiss.com)
- This high quality confocal system is driven by the powerful software ZEISS Efficient Navigation (ZEN) to bring you the added benefits of maximum user comfort and increased productivity. (zeiss.com)
- Benefit from a fully integrated system: optics, electronics and camera are all enclosed in the microscope. (zeiss.com)
- 3D-printed optics system Printoptical Technology will "democratize the way optics are made. (optics.org)
- Fraunhofer FIT develops sensitive confocal microscope system for analyzing nucleic acid behavior. (optics.org)
- The confocal system has been used for over 4,500 hours. (edu.pl)
Fluorescently1
- This confocal microscope enables the simultaneous detection of various fluorescently labelled proteins and their co-localization in examined specimens. (edu.pl)
Phase contrast1
- PHASE CONTRAST: Optics are used to emphasize slight diff. (freezingblue.com)
Detector1
- This is achieved by the correlated aperture spinning disc technology integrated in the Smartproof 5 detector module, while maintaining the typical benefits of confocal microscopes for surface characterization. (zeiss.com)
Capability1
- The scientific objective of this equipment acquisition is to establish a state-of-the-art experimental optics capability at Northwestern University for understanding hybrid materials with tailored properties emerging from the combination of low-dimensional electronic systems with distinct characteristics arising from symmetry, structure, or external control. (northwestern.edu)
Resolution9
- Because of its patented widefield, correlated aperture confocal technology, your Smartproof 5 minimizes the time to result thus providing a perfect balance between high resolution and high speed. (zeiss.com)
- Resolution is the ability of the microscope to distinguish two objects from each other. (opticsmag.com)
- Microscopes with higher resolution are capable of achieving greater magnification without blurring the image. (opticsmag.com)
- The correct handling of the microscope and optimising image quality (e.g. contrast, resolution) as well as experimental design according to different applications are crucial to obtain the best results. (conference-service.com)
- This DURIP proposal seeks funding to acquire a cryogenic magneto-optical confocal microscope that will combine low-temperature, high-magnetic fields, and high-resolution microscopy for exploring nano-scale materials and heterostructures. (northwestern.edu)
- Even today, new modes and applications arise, such as super-resolution fluorescence microscopy, the development of which was awarded the 2014 Nobel Prize in Chemistry. (bioinfoinc.com)
- And in optical microscopy, the donut delivered a powerful blow to a stubborn paradigm curtailing the resolution of microscopes for decades. (abberior.rocks)
- That is, there is an inherent limit to the resolution of a microscope dictated by the very nature of the signal it detects. (abberior.rocks)
- The mundane donut bumped up the resolution power of microscopes ten-fold and beget the field of superresolution microscopy. (abberior.rocks)
Contrast1
- In contrast, a thin optical section of the same grain (Figure 1(f)) acquired with confocal techniques displays a dramatic difference between the particle core and the surrounding envelope. (olympusconfocal.com)
Magnification2
- Magnification is a measure of how much larger an object appears when you view it through the microscope. (opticsmag.com)
- Each one offers a different level of magnification, allowing you to quickly change your view without making major alterations to the microscope or your slide. (opticsmag.com)
ZEISS4
- The best of ZEISS optics and proven components enable you to work effectively across a broad range of applications. (zeiss.com)
- The new ZEISS objective class C Epiplan-Apochromat has been especially designed for widefield confocal systems. (zeiss.com)
- Carl Zeiss MicroImaging Online Campus - Visit the new ZEISS website that explores the fascinating world of optical microscopy and provides the necessary background to understand both the basic concepts and advanced principles. (fsu.edu)
- A Stereo Microscope from Carl Zeiss is ideal for analysis of rough materials surfaces. (antisel.gr)