Microsatellite. Medical search. Frequent questions

Thus, the mutation rate at microsatellite loci is expected to differ from other mutation rates, such as base substitution rates. (wikipedia.org)
The mutation rate at microsatellite loci depends on the repeat motif sequence, the number of repeated motif units and the purity of the canonical repeated sequence. (wikipedia.org)
Our microsatellite barcoding method exploits the fact that microsatellites in various loci undergo length changes during cell division, to create a natural barcode for each cell, and reconstruct the overall differentiation tree using a phylogenetic algorithm. (europa.eu)
3-4 To validate MSI testing via NGS, Caris evaluated more than 7,000 target microsatellite loci and compared the results from PCR for 2,189 cases across 26 different tumor types. (carislifesciences.com)
Samples were genotyped with 13 microsatellite and 29 SNP assays and average genotyping success for good, intermediate, and poor quality samples was 96%, 24%, and 24% for microsatellite loci, and 98%, 97%, and 79% for SNPs, respectively. (critfc.org)
Logistic regression of genotyping success by starting copy number indicated that in order to achieve at least 90% genotyping success, approximately 1,000 starting copies of nuclear DNA are necessary for microsatellite loci, and as few as 14 starting copies for SNP assays (but we recommend at least 50 copies to reduce genotyping error). (critfc.org)
Using the data from both 28 species where genome sequencing is advanced and 76 species from which marker loci have been published, we show that in mammals, maximum repeat number is inversely correlated with body temperature, with warmer-blooded species having shorter 'long' microsatellites. (bas.ac.uk)
Population differentiation and relationships among 6 natural rockfish populations collected from northern coastal seas around Japan were assayed using microsatellite DNA loci. (eurekamag.com)
The objective of this work was to verify the effect of natural selection on microsatellite alleles as indicators of better adaptation and identification of quantitative trait loci (QTLs) for grain yield. (ejbiotechnology.info)
Of the 30 microsatellite loci, number of alleles per locus (Na) and effective number of alleles per locus (Ne) ranged from 4 to 11 and 2.157 to 8.019, respectively. (scialert.net)
Despite this variation, we provide an indication of what number of sequences and consequent proportion of a 454 run are required for the development of 40 designable, unique microsatellite loci for a typical molecular ecological study. (datadryad.org)
Finally, to address the challenges of choosing loci from the vast array of microsatellite loci typically available from partial genome runs (average for this study, 2341 loci), we provide a microsatellite development flowchart as a procedural guide for application once the results of a partial genome run are obtained. (datadryad.org)
In the first part of the thesis novel microsatellite loci for the buff-tailed bumblebee Bombus terrestris are developed and characterized to be available for this and other bumblebee species. (uni-halle.de)
In total, 31 polymorphic microsatellite loci of different motifs and composition were developed. (sun.ac.za)

The increasing availability of DNA amplification by PCR at the beginning of the 1990s triggered a large number of studies using the amplification of microsatellites as genetic markers for forensic medicine, for paternity testing, and for positional cloning to find the gene underlying a trait or disease. (wikipedia.org)
To date, highly variable markers such as microsatellites have only been developed for the photobionts of two lichen species, namely Lobaria pulmonaria (L.) Hoffm. (bioone.org)
microsatellite markers are available for the species (Bro wn et al. (researchgate.net)
Our technique, which is highly detailed with respect to current versions of microsatellite barcoding, provides high dimensional characterization of the cells and will allow the inference of important properties such as new phenotypic markers and key molecular regulators. (europa.eu)
A battery of 14 microsatellite markers was used to carry out parentage tests of 256 progeny individuals including two independent samples of selected trees and one control unselected sample, all derived from 6-year-old forest stands in eastern Brazil. (springer.com)
Brondani RPV, Brondani C, Tarchini R, Grattapaglia D (1998) Development and mapping of microsatellite based markers in Eucalyptus. (springer.com)
Putative microsatellite and SNP markers were identified. (nih.gov)
A total of 720 individuals of 12 indigenous chicken populations, geographically localized in Southern China were genotyped for 30 microsatellite markers in polymerase chain reaction (PCR) to evaluate the genetic variation and genetic distance between populations. (scialert.net)
Next generation sequencing (NGS) is revolutionizing molecular ecology by simplifying the development of molecular genetic markers, including microsatellites. (datadryad.org)
Here we summarize the results of the large scale development of microsatellites for 54 non-model species using NGS and show there are clear differences amongst plants, invertebrates and vertebrates for the number and proportion of motif types recovered that are able to be utilised as markers. (datadryad.org)
The genetic characterization of 117 peach and nectarine cultivars ( Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. (ejbiotechnology.info)
Alice: Transcriptome analysis of Euterpe edulis and identification of microsatellite markers. (embrapa.br)
Nine microsatellite markers were used to genotype juveniles and potential parents, with assignment completed using CERVUS 2.0. (sun.ac.za)
Paternity identification, based on microsatellite molecular markers, established that the progeny produced in year 2 by females mated only in year 1, were often fathered by more than one male. (who.int)
ABSTRACT This study aimed to identify the status of 2 major microsatellite instability markers (repair genes hMSH2 and hMSH6 ) in colorectal cancer cases operated at King Khalid University Hospital, Riyadh, Saudi Arabia between 2007 and 2009. (who.int)
Methods: GWS was performed using 811 microsatellite markers providing about five centiMorgan (cM) resolution. (lu.se)

Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists and in genetic genealogy, or as simple sequence repeats (SSRs) by plant geneticists. (wikipedia.org)
Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. (wikipedia.org)
citation needed] Unlike point mutations, which affect only a single nucleotide, microsatellite mutations lead to the gain or loss of an entire repeat unit, and sometimes two or more repeats simultaneously. (wikipedia.org)
Microsatellite instability (or MSI) is defined by the National Cancer Institute as a change that occurs in the DNA of certain cells, such as tumor cells, in which the number of repeats of microsatellites (short, repeated sequences of DNA) is different from the number of repeats that was in the DNA when it was inherited. (merck.com)
SSR_pipeline is a flexible set of programs designed to efficiently identify simple sequence repeats (e.g., microsatellites) from paired-end high-throughput Illumina DNA sequencing data. (usgs.gov)
The microsatellite search algorithm is extremely efficient, and we have used it to identify repeats with motifs from 2 to 25bp in length. (usgs.gov)

We have 3153 products for the study of Low-frequency Microsatellite Instability that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits. (novusbio.com)
Immunohistochemistry versus microsatellite instability testing for screening colorectal cancer patients at risk for hereditary nonpolyposis colorectal cancer syndrome. (carislifesciences.com)
A retrospective study of consistency between immunohistochemistry and polymerase chain reaction of microsatellite instability in endometrial cancer. (bvsalud.org)
Both MMR protein immunohistochemistry and microsatellite instability (MSI) analysis can be used. (lu.se)
In a study of 450 CRC diagnosed under 50 years via the Ohio Colorectal Cancer Prevention Initiative, germline DNA analysis for mutations in 25 cancer susceptibility genes was done in addition to assessing microsatellite instability and/or immunohistochemistry for MMR deficiency. (cdc.gov)
A recent study compared the performance of tumor next-generation sequencing to the current multistep approach (using microsatellite instability (MSI) testing and/or immunohistochemistry (IHC) for MMR proteins). (cdc.gov)

Research of Low-frequency Microsatellite Instability has been linked to Microsatellite Instability, High-frequency Microsatellite Instability, Malignant Neoplasms, Neoplasms, Colorectal Cancer. (novusbio.com)
In May, 2017, the FDA granted accelerated approval for the use of Keytruda for treatment of patients with unresectable or metastatic solid tumors having either microsatellite instability-high (MSI-H) or mismatch repair deficient (dMMR) biomarkers. (cms.gov)
Identification of endometrial cancers (EC) with mismatch repair deficiency (dMMR) or microsatellite instability -high (MSI-H) is essential for Lynch syndrome screening and treatment stratification. (bvsalud.org)

Although the first microsatellite was characterised in 1984 at the University of Leicester by Weller, Jeffreys and colleagues as a polymorphic GGAT repeat in the human myoglobin gene, the term "microsatellite" was introduced later, in 1989, by Litt and Luty. (wikipedia.org)
All microsatellites were found to be polymorphic. (scialert.net)

Using a shuttle vector assay, we measured mutation rates at reporter cassettes containing defined mononucleotide, dinucleotide, and tetranucleotide microsatellite sequences. (nih.gov)
The modules are used to 1) identify the subset of paired-end sequences that pass Illumina quality standards, 2) align paired-end reads into a single composite DNA sequence, and 3) identify sequences that possess microsatellites (both simple and compound) conforming to user-specified parameters. (usgs.gov)
A total of 35 microsatellites were identified from the ESTs, with 18 having sufficient flanking sequences for primer design. (nih.gov)

Other microsatellites are located in regulatory flanking or intronic regions of genes, or directly in codons of genes - microsatellite mutations in such cases can lead to phenotypic changes and diseases, notably in triplet expansion diseases such as fragile X syndrome and Huntington's disease. (wikipedia.org)
In the absence of hPMS2, a greater number of microsatellite expansion versus deletion mutations was observed, and expansion rates of the tetranucleotide alleles were similar. (nih.gov)
These diseases are all caused by microsatellite mutations, where a microsatellite either has three bases, four bases, five bases, or six bases, and these mutations result in excess numbers of copy of these particular units. (hstalks.com)
These tumors may show microsatellite instability and have mutations in PTEN , PIK3CA , KRAS , and CTNNB 1. (msdmanuals.com)

MsatAllele_1.0: An R package to visualize the binning of microsatellite alleles. (ualg.pt)

It has been also hypothesized that mismatch repair-deficient/microsatellite-unstable tumors are more responsive to PD-L1 blockade. (medscape.com)

Microsatellites occur at thousands of locations within an organism's genome. (wikipedia.org)
Microsatellites are distributed throughout the genome. (wikipedia.org)
The human genome for example contains 50,000-100,000 dinucleotide microsatellites, and lesser numbers of tri-, tetra- and pentanucleotide microsatellites. (wikipedia.org)
In the second part these and further microsatellites are used to map the genome of the buff-tailed bumblebee. (uni-halle.de)

2015). MMR results in a strong mutator phenotype known as microsatellite instability (MSI), which is a hallmark of Lynch syndrome-associated cancers (Yamamoto & Imai, 2015). (alliedacademies.org)

Results: Among the 97 urothelial tumors, 86 (88.7%) showed immunohistochemical MMR loss and 68 were successfully analyzed by the Promega MSI assay, of which 48 (70.6%) were MSI-high and 20 (29.4%) were MSI-low/microsatellite stable. (lu.se)
In a phase II study of pembrolizumab in patients with microsatellite-high (MSI-H) solid tumors, the objective response rate was 39.6% (95% CI = 31.7% to 47.9%), including 7.4% complete responses (CRs) and 32.2% partial responses (PRs). (medscape.com)

Over 90% of all colorectal cancers in HNPCC patients demonstrate a high microsatellite instability (MSI-H), which means at least 2 or more genes have been mutated in HNPCC families or atypical HNPCC families. (medscape.com)

Performance of microsatellites for parentage assignment following mass controlled pollination in a clonal seed orchard of loblolly pine (Pinus taeda L. (springer.com)

The objectives of this study were to construct a normalized cDNA library for efficient EST analysis, to generate thousands of ESTs, and to analyze the ESTs for microsatellites and potential single nucleotide polymorphisms (SNPs). (nih.gov)

A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from one to six or more base pairs) are repeated, typically 5-50 times. (wikipedia.org)
1992). A microsatellite is a tract of tandemly repeated (i.e. adjacent) DNA motifs that range in length from one to six or up to ten nucleotides (the exact definition and delineation to the longer minisatellites varies from author to author), and are typically repeated 5-50 times. (wikipedia.org)
Similarly, insects have shorter repeat motifs in their telomeres that could arguably be considered microsatellites. (wikipedia.org)

For example, the sequence TATATATATA is a dinucleotide microsatellite, and GTCGTCGTCGTCGTC is a trinucleotide microsatellite (with A being Adenine, G Guanine, C Cytosine, and T Thymine). (wikipedia.org)

Today I'm going to tell you about microsatellites, and trinucleotide repeat expansions diseases. (hstalks.com)

Immunohistochemical study of microsatellite instability was done with antibodies to hMSH2 and hMSH6 . (who.int)

These pathways complement our catalog of research reagents for the study of Low-frequency Microsatellite Instability including antibodies and ELISA kits against MLH1, SIL1, MSH2, MSI, MSH6. (novusbio.com)

Most patients are microsatellite stable, and a few are microsatellite instable [MSI]. (ajmc.com)

Fast-forward another four years, and Cantrell predicts we'll see as many as 500 microsatellites making the trip into orbit, comprising three out of every four satellites launched in 2020. (foxbusiness.com)

Prominent early applications include the identifications by microsatellite genotyping of the eight-year-old skeletal remains of a British murder victim (Hagelberg et al. (wikipedia.org)

Microsatellites are also used in population genetics to measure levels of relatedness between subspecies, groups and individuals. (wikipedia.org)

Most eukaryotes have microsatellites, with the notable exception of some yeast species. (wikipedia.org)

The mismatch repair (MMR) system plays a major role in removing DNA polymerization errors, and loss of this pathway results in hereditary cancers characterized by microsatellite instability. (nih.gov)
RÉSUMÉ La présente étude visait à identifier le statut de deux marqueurs principaux de l'instabilité des microsatellites (gènes de réparation hMSH2 et hMSH6 ) dans des cas de cancers colorectaux opérés à l'hôpital universitaire King Khalid de Riyad (Arabie saoudite) entre 2007 et 2009. (who.int)

CYGNSS will be an octet of 64-lb. (29-kg) microsatellites, each slightly larger than a carry-on suitcase, set in Earth orbit to monitor developing hurricanes and tropical cyclones. (space.com)
Each microsatellite can measure the reflections from four spots simultaneously as they orbit through the tropics. (space.com)
Space start-ups -- and SpaceX itself -- see a future for small, cheap microsatellites in orbit. (foxbusiness.com)
Cantrell's outfit, for example, will begin taking reservations later this year to launch 50- to 100-pound microsatellites into orbit for $1.5 million to $2.5 million apiece. (foxbusiness.com)
As recently as 2012, we were sending only a dozen or three microsatellites into orbit annually. (foxbusiness.com)
This year, a new record could be set -- perhaps as many as 210 microsatellites sent into orbit, or six times 2012's tally. (foxbusiness.com)
Taking a different route to space is Stratolaunch Systems, which plans to fly an airplane really high, drop a rocket with a microsatellite aboard it, and let the rocket boost thesatellite the rest of the way into orbit -- missile style. (foxbusiness.com)
Vector Space Systems is the outlier here, offering space launch for as little as $1.5 million and promising to begin operations as early as 2018, while Virgin Galactic is offering the most robust capability -- $10 million to put a 440-pound microsatellite into a 300-mile-high sun-synchronous orbit. (foxbusiness.com)
After all, were SpaceX to imitate Rocket Lab's business plan, one single Falcon 9 rocket could easily lift the mass of 100 or more 100-pound microsatellites into orbit. (foxbusiness.com)
The vehicle would then fire its four main engines and launch into low-Earth orbit to deploy one or more microsatellites weighing up to a total of 100 pounds (45 kilograms). (defense-update.com)

Caris molecular profiling includes microsatellite instability (MSI) testing via next-generation sequencing (NGS). (carislifesciences.com)
We further show that the Illumina platform is capable of identifying large numbers of microsatellites, even when using unenriched sample libraries and a very small percentage of the sequencing capacity from a single DNA sequencing run. (usgs.gov)
recommendations for ecologists when using next generation sequencing for microsatellite development. (datadryad.org)
Data from: Rise of the machines - recommendations for ecologists when using next generation sequencing for microsatellite development. (datadryad.org)
Sixty-one percent of sequenced clones were deemed redundant and pre-screening for both uniqueness and the presence of microsatellites would reduce unnecessary sequencing thus improving the efficiency of the FIASCO method and reducing costs. (sun.ac.za)

The results provided evidence of the applicability of microsatellite to determining the genetic relatedness among different Chinese indigenous chicken populations and evaluating of genetic variations. (scialert.net)

Every patient with colon cancer and every physician treating colon cancer needs to know the microsatellite status of all patients. (ajmc.com)
Although promising, no immunotherapy drugs have been approved by the FDA to treat gastrointestinal malignancies outside of the recent approval of pembrolizumab for the treatment of patients with colon cancer with microsatellite instability (MSI). (medscape.com)

NASA's eight Cyclone Global Navigation Satellite System (CYGNSS) microsatellites will launch and deploy Dec. 12, 2016, to study the evolution of hurricanes and cyclones in Earth's tropical hurricane belt. (space.com)

Boeing has said that it intends to slash the cost of microsatellite launches by 66 percent. (spacenews.com)
The building's lab area accommodates the assembly and testing of microsatellites while the office space supports the design, development and the monitoring of microsatellites after their mission launches. (archello.com)

If [they're] microsatellite instable, then do genetic analysis to confirm and make sure you do germline testing to see if the patient is has Lynch syndrome. (ajmc.com)
The report also describes the main companies involved in Nanosatellite And Microsatellite analysis products and lists all of their large and minor projects. (digitaljournal.com)
Nanosatellite And Microsatellite Market report provides an absolute overview of the market by covering many elements of market analysis, product definition, market segmentation, significant developments, and the existing vendor landscape through 2027. (digitaljournal.com)
Moreover, customer preference analysis, market dynamics (drivers, restraints, opportunities), new product release, impact of COVID-19, and regional conflicts provide crucial information for you to take a deep dive into the Nanosatellite And Microsatellite market. (digitaljournal.com)
This Nanosatellite And Microsatellite Market Report offers analysis and insights based on original consultations with important players such as CEOs, Managers, Department Heads of Suppliers, Manufacturers, and Distributors etc. (digitaljournal.com)

NASA scientists are gearing up for the Dec. 12 launch of the Cyclone Global Navigation Satellite System (CYGNSS), a dedicated fleet of storm-watching microsatellites that will track hurricane evolution over Earth's oceans. (space.com)
When the microsatellites are deployed Dec. 12, their launch vehicle, the Pegasus, will be dropped from an L-1011 aircraft at 40,000 feet , where it will wait 5 seconds before the rocket engines ignite. (space.com)
Hypothetical prices on these hypothetical services vary (none are operational as of yet), but most of the start-ups seem to be quoting prices in the $5 million to $10 million range for microsatellite launch services. (foxbusiness.com)
The U.S. Defense Advanced Research Projects Agency (DARPA) recently released this 2.5-minute animation showing how the U.S. could launch microsatellites quickly and economically using an F-15-deployed rocket Boeing is developing under the Airborne Launch Assist Space Access (ALASA) program. (spacenews.com)
Iceye, a space technology startup in Helsinki, Finland, has secured more than $5 million in funding to help launch radar-based microsatellites designed to deliver earth imagery for disaster relief, logistics, planetary exploration, agriculture, nature, and climate applications. (militaryaerospace.com)

Dr Marshall describes the impacts of microsatellite stability and instability on metastatic colorectal cancer. (ajmc.com)

The microsatellite industry is growing and the ALASA promises this market a quick and cost-efficient way of launching such satellites. (spacenews.com)

The research explores prominent Nanosatellite And Microsatellite market players in order to better understand their market position and future strategies. (digitaljournal.com)

Our results support a model of microsatellite evolution in which maximum length is limited by a temperature-dependent stability threshold. (bas.ac.uk)

The study of Low-frequency Microsatellite Instability has been mentioned in research publications which can be found using our bioinformatics tool below. (novusbio.com)
In this project, an imager study and orbital design of a microsatellite constellation using near infrared (NIR) and shortwave infrared (SWIR) imagers for wildfire monitoring, with enhanced spatial and temporal resolution, was conducted. (kth.se)
The purpose of the market research study is to thoroughly evaluate the Information and Communication Technology , Information Technology sector and gain a review about the Nanosatellite And Microsatellite industry and its commercial possibilities. (digitaljournal.com)
In the next study the evolutionary dynamics of microsatellites was investigated in Diptera and Hymenoptera. (uni-halle.de)

The Microsatellite Science & Technology Centre (MSTC) for the University of Toronto Institute for Aerospace Studies (UTIAS) is a networking hub for Canadian researchers developing nanotechnology and microsatellites for use in space. (archello.com)
The Nanosatellite And Microsatellite Market research report is the result of persistent work conducted by qualified forecasters, creative analysts, and brilliant researchers. (digitaljournal.com)

The Nanosatellite And Microsatellite Market Research Report 2022-2027, provides an in-depth overview and insights into the market size, revenues, various segments and drivers of development, as well as limiting factors and regional industrial presence. (digitaljournal.com)
The report focuses on the Nanosatellite And Microsatellite market size, segment size (mainly covering product type, application, and geography), competitor landscape, recent status, and development trends. (digitaljournal.com)
For the microsatellite development, 50% of positive recombinant clones contained inserts. (sun.ac.za)

Explore more on Low-frequency Microsatellite Instability below! (novusbio.com)

Anatomy22

Organisms20

Diseases16

Chemicals and Drugs19

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Psychiatry and Psychology1

Phenomena and Processes97

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture2

Information Science3

Health Care10

Geographicals7

Loci14

Markers16

Repeats6

Immunohistochemistry6

Instability-high3

Polymorphic2

Sequences3

Mutations4

Alleles1

Mismatch repair deficient1

Genome4

Mutator phenotype1

Tumors2

Genes1

Parentage assignment1

Single nucleotide1

Motifs3

Dinucleotide1

Trinucleotide1

Immunohistochemical1

MSH61

Stable1

20201

Genotyping1

Population genetics1

Species1

Cancers2

Orbit10

Sequencing5

Relatedness1

Colon cancer2

Deploy1

Launches2

Analysis5

Launch5

Marshall1

Promises1

Prominent1

Results1

Study4

Researchers2

Development3

Explore1