Microbiol resistance genes bacterial. Medical search. Frequent questions

He demonstrated that the antibiotic streptomycin acts by inhibiting the 30S ribosome's role in protein synthesis, that this inhibition causes misreading of the genetic code, and that the most common mode of resistance reduced the accuracy of protein synthesis. (wikipedia.org)
At the University of Wisconsin he returned to his original focus on antibiotics and resistance, showing that plasmid-borne streptomycin resistance genes act by inactivating the antibiotic rather than by modifying protein synthesis. (wikipedia.org)
These broad-ranging studies led to a deeper understanding of the bacterial ribosome and of how antibiotic resistance genes arise and spread in both bacteria and yeast. (wikipedia.org)
Other useful products of the lab's research include the restriction enzymes PstI and KpnI and the npt gene conferring resistance to the antibiotic G418 widely used in research. (wikipedia.org)
Origins and evolution of antibiotic resistance. (wikipedia.org)
Shotgun metagenomics revealed a total of 112 different mobile antibiotic resistance gene types, conferring resistance against almost all classes of antibiotics. (biomedcentral.com)
The rise in antibiotic resistance is a serious growing problem for human health [ 1 ]. (biomedcentral.com)
In summary, we demonstrated that P. aeuginosa clinical isolates with novel epigenetic markers could form excessive biofilm, which might enhance its antibiotic resistance and in vivo colonization in COVID-19 patients. (frontiersin.org)
Although attractive fermentation performances were reached, significant improvements are required to eliminate recombinant plasmids, antibiotic resistances and inducible promoters, and increase ethanol tolerance. (biomedcentral.com)
Antibiotic resistance has been classified by the World Health Organization as one of the three major public health threats of the 21st century (6). (scielo.br)
The rapid emergence of antibiotic resistance among bacteria is, to a great extent, due to the dissemination of antibiotic resistance genes by horizontal transfer mediated by plasmids, transposons and integrons (5). (scielo.br)
Although, almost all previous studies and efforts to control the dissemination of these genes have been based on isolates from clinical samples, antibiotic resistance genes can also occur in nonpathogenic bacteria, which can then be transferred via lateral gene transfer (6). (scielo.br)
The antibiotic resistance property is hazardous in itself (whether with or without the contaminants being pathogenic) considering the potential for interspecies transfer of these resistance traits (be they chromosomal or plasmid-borne) to commensal or pathogenic bacteria in clinical settings and/or in the food chain. (researchsquare.com)
The epidemiology of food-borne infections is changing, including its several manifestations: emergence of new and more virulent pathogenic bacterial clades, regional or global spread, and dissemination of antibiotic resistance [7, 8]. (researchsquare.com)
Noise reduction strategies in metagenomic chromosome confirmation capture to link antibiotic resistance genes to microbial hosts. (cdc.gov)
Thus, the main global objectives of SATURN were to study the impact of antibiotic exposure on antimicrobial resistance (AMR) and to define strategies to improve knowledge on antibiotic selection pressure and judicious antibiotic use. (europa.eu)
We describe enterococci in raw-frozen dog food commercialized in Europe as a source of genes encoding resistance to the antibiotic drug linezolid and of strains and plasmids enriched in antibiotic-resistance and virulence genes in hospitalized patients. (escmid.org)
Diversity of metal and antibiotic resistance genes in Enterococcus spp. (escmid.org)
for their recent publication involving EFWISG members from different countries and showing the accretion of metal and antibiotic resistance in Enterococcusspp. (escmid.org)
The Star Tribune Editorial, "Renew the battle vs. antibiotic resistance" (Dec 2nd 2022) supports the legislation dubbed the 'Pioneering Antimicrobial Subscriptions to End Upsurging Resistance" (PASTEUR) Act now before Congress would provide drug companies with $6 billion to develop new antibiotics. (drfoxonehealth.com)
Evidence of the obesogenic effects of antibiotics and their disruption of healthy gut microbiomes leading to a number of health problems are now surfacing along with evidence that some non-antibiotic pharmaceuticals can enhance bacterial antibiotic resistance. (drfoxonehealth.com)
An analysis of human deaths associated with antibiotic resistant bacteria published in The Lancet on 19 January 2022, estimates that in 2019, 4.95 million people died from illnesses in which bacterial antimicrobial resistance (AMR) played a part. (drfoxonehealth.com)
Ding P, Lu J, Wang Y, Schembri MA, Guo J. Antidepressants promote the spread of antibiotic resistance via horizontally conjugative gene transfer. (drfoxonehealth.com)
These animal-borne threats include viruses as well as new forms of antibiotic resistance genes and the multi-drug resistant superbugs that carry them. (drfoxonehealth.com)
Infant gut commensals risk transferring multidrug-resistant antibiotic resistance genes (ARGs) to pathogenic bacteria. (biomedcentral.com)
all infants harboured these antibiotic resistance genes at some point in the first year of life. (biomedcentral.com)
In this longitudinal cohort study of infants living in a region with high endemic antibacterial resistance, we demonstrate that majority of the infants harboured several antibiotic resistance genes in their gut and showed that the infant gut resistome is diverse and dynamic over the first year of life. (biomedcentral.com)
However, the gut microbiota is a reservoir for antibiotic resistance genes (ARGs). (biomedcentral.com)
This is the first study in the region describing changes in the total antibiotic resistance gene (ARG) profile (also known as the resistome) of healthy infants over time. (biomedcentral.com)
Antibiotic Resistance Patterns and Serotypes of Salmonella spp. (ophrp.org)
Few long-term studies have been conducted on the serotype and antibiotic resistance patterns of Salmonella speices (spp. (ophrp.org)
The aim of this study was to determine the serotypes and antibiotic resistance patterns of Salmonella spp. (ophrp.org)
The most highly observed antibiotic resistance pattern among Salmonella spp. (ophrp.org)
Overall, S . Enteritidis and S . Typhimurium showed higher antibiotic resistance than the other Salmonella serotypes tested in this study. (ophrp.org)
Based on global surveillance results, the World Health Organization [ 17 ] reported that the antibiotic resistance of Salmonella spp. (ophrp.org)
13 ] reported that the patterns of bacterial antibiotic resistance are constantly changing, and the emergence of multi-drug-resistant Salmonella threatens public health worldwide [ 18 ]. (ophrp.org)
Here we evaluate public datasets of soil metagenomes mining for changes in antibiotic resistance genes (ARGs) of soils from sugarcane mesocosms repeatedly amended with vinasse. (biomedcentral.com)
However, recent research has demonstrated that the utilization of organic compounds as fertilizers in agriculture soils can alter the selective pressure that drives antibiotic resistance genes (ARGs) on soil-borne microbial communities [ 4 ]. (biomedcentral.com)
Four years of monitoring antibiotic resistance in microorganisms from bacteremic patients. (ac.ir)
In Staphylococcus aureus, the phage-mediated acquisition of mobile genetic elements (MGEs) that encode virulence and antibiotic resistance genes largely contribute to its evolutionary adaptation and genetic plasticity. (bvsalud.org)
Evidence for wastewaters as environments where mobile antibiotic resistance genes emerge. (janusinfo.se)
ABSTRACT The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the bla gene that encodes for carbapenemase production have complicated the management of patient infections. (who.int)
MDR was defined as resistance to at least one antibiotic in three or more categories, while selected MDR bacteria such as ESBL was identified using double disk synergy test, and MRSA by cefoxitin disk diffusion test. (bvsalud.org)

Furthermore, nucleotide sequences related to the cluster vanHAX are present in this DNA, suggesting that the prolonged use of avoparcin in agriculture led to the uptake of glycopeptide resistance genes by animal commensal bacteria, which were subsequently transferred to humans. (cdc.gov)
Neither approach relies on the host-bacteria being cultivable, but both largely lack the ability to differentiate between mobilized and non-mobilized genes. (biomedcentral.com)
While acquired resistance has received considerable attention, relatively little is known of intrinsic resistance that allows bacteria to naturally withstand antimicrobials. (frontiersin.org)
The helper-drug can target gene products that by any mechanism aid bacteria to resist higher concentrations of an antimicrobial ( Pieren and Tigges, 2012 ). (frontiersin.org)
Besides the ability of bacteria to acquire antimicrobial resistance via horizontal gene transfer or spontaneous mutations, they can also be intrinsically resistant to antimicrobials ( Cox and Wright, 2013 ). (frontiersin.org)
Therefore, biofilm matrix gives the additional resistance power to bacteria which makes them to not only tolerate harsh conditions but also resistant to antibiotics which lead to the emergence of bad bugs infections like multi drug resistant, extensively drug resistant and totally drug resistant bacteria. (biomedcentral.com)
The bacteria were tested for siderophore and indole-3-acetic acid (IAA) production, phosphate solubilization, and presence of the nif H gene. (scielo.br)
More than 70% of the evaluated bacteria presented the ability of siderophore production and phosphate solubilization, and possessed the nif H gene. (scielo.br)
Antimicrobial Resistance (AMR) is rampant among bacteria that cause healthcare- and community-acquired infections, driving up costs and increasing the difficulty of therapeutic management. (europa.eu)
Tetracycline HCl inhibits bacterial growth in Gram-positive and Gram-negative bacteria by disrupting codon-anticodon interactions at the ribosome, thus blocking protein synthesis. (toku-e.com)
A few species of bacteria display intrinsic resistance to tetracycline, including Pseudomonas aeruginosa . (toku-e.com)
1. Introduction Mercury resistance genes (mer determinants) provide a suitable model system for studying gene transfer in environmental bacteria. (dadospdf.com)
Resistance to mercury salts is found in many genera of bacteria isolated from the environment, and many mer determinants have been sequenced. (dadospdf.com)
The ability of bacteria to survive antimicrobial stress is of major importance because, if current trends continue, it is predicted that by 2050 10 million people will die annually due to anti-microbial resistance ( 1 ). (biorxiv.org)
Mechanisms of antimicrobial action and resistance in bacteria are complex and often vary depending on growth phase and/or concentration of the antimicrobial applied. (biorxiv.org)
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis Natalia Comella and Alan D. Grossman* Department of Biology, Building 68-530, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. (igem.org)
Bacteriophage transduction is the major mechanism of horizontal gene transfer (HGT) among many bacteria. (bvsalud.org)
Despite phage replication in these matrices, lysis of the bacteria was slower than phage propagation, and no reduction of the bacterial population was observed. (bvsalud.org)
It induces both local effects in the gut, as well as alterations in distant organs through stimuli generated by bacteria, structural bacterial components, and microbial metabolites [ 7 ]. (biomedcentral.com)
NDM-1 (New-Delhi-Metallo-β-lactamase-1) is an enzyme developed by bacteria that is implicated in bacteria resistance to almost all known antibiotics. (degruyter.com)
Data are scarce on the prevailing Streptococcus pneumoniae serotypes in the Philippines, including the relative antimicrobial resistance (AMR) of these bacteria. (who.int)
Background: Today, bacterial resistance is a public health challenge throughout the world, and infections caused by resistant bacteria are associated with increased morbidity, mortality and health care costs. (bvsalud.org)
Conclusion: This study reports high prevalence of MDR bacteria, mainly ESBL-E, with concerning high resistance to carbapenem. (bvsalud.org)
In 2014, the MetaHIT consortium by bacteria of the genera Lacto- alytical method used: 66 from 16S published an integrated catalogue bacillus , Prevotella , and Sneathia , rDNA sequencing [11] or 57 from of 10 million bacterial genes de- whereas for infants delivered by whole-metagenome sequencing [5]. (who.int)

The first description of isolates with -lactamase OXA- are infections with carbapenem-susceptible Enterobacte- 48-like ( bla -like) genes in the United States was from OXA-48 riaceae ( 3 ). (cdc.gov)
This study was aimed at determining the urinary bacterial isolates and their susceptibility to selected antibiotics among pregnant women attending antenatal clinics of selected Hospitals in Ilorin, Nigeria. (who.int)
Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance ( mec A) gene was detected by conventional PCR assay. (who.int)
Gram negative isolates showed high resistance rate of 73.1% to ampicillin and 65.4% to amoxicillin-clavulanic acid while Gram-positive isolates showed high resistant rate of 94.1% to penicillin. (who.int)
Four of the 9 (44.4%) CoNS isolates were cefoxitin resistant and all the 4 (100%) carried mec A gene. (who.int)
Presence and phenotypic expression of blaTEM, blaSHV, and blaCTX-M ESBL-encoding genes were investigated in environmental water isolates of Aeromonas hydrophila and Aeromonas jandaei. (scielo.br)
Presence of blaSHV and blaCTX-M genes was not observed, and blaTEM gene was verified in 91% of the isolates. (scielo.br)
Presence of bla SHV and bla CTX-M genes was not observed, and bla TEM gene was verified in 91% of the isolates. (scielo.br)
The aim of this study was to investigate the presence and phenotypic expression of bla TEM , bla SHV, and bla CTX-M ESBL- encoding genes in 87 environmental water isolates of Aeromonas hydrophila (n=41) and Aeromonas jandaei (n=46). (scielo.br)
Moreover, all of the isolates exhibited intermediate-level or higher resistance to two or more clinically relevant antibiotics. (researchsquare.com)
Genetic diversity of the bacterial isolates was evaluated by rep-PCR. (scielo.br)
Gram-positive, spore-forming bacilli comprised most bacterial isolates. (scielo.br)
In addition to the existing problem with VRE, the potential emergence of vancomycin resistance in clinical isolates of Staphylococcus aureus or Staphylococcus epidermidis is a serious public health concern. (cdc.gov)
This study investigated whether cell-free supernatants (SN) from four bovine non-aureus staphylococcal (NAS) isolates prevent Staphylococcus aureus adhesion to and internalization into bovine mammary epithelial cells (MAC-T cells) and if so, to determine whether such effects were potentially associated with the S. aureus accessory gene regulator (agr) system. (bvsalud.org)
All 48 Salmonella isolates carried intrinsic and acquired resistant genes such as aac.6…Iaa, mdf(A), qnrB, qnrB19 genes and golT, golS, pcoA, and silP, mediated by plasmid Col440I_1, incFIB.B and incFII. (biomedcentral.com)
Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002). (ac.ir)
Distribution of the exfoliative toxin D gene in clinical Staphylococcus aureus isolates in France. (medscape.com)
This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the bla gene and compared the results with modified Hodge test. (who.int)
The high prevalence of ertapenem nonsusceptibility at a tertiary care hospital in Egypt was predominantly attributed to K. pneumoniae carbapenemase-mediated resistance mechanisms in K. pneumoniae isolates. (who.int)

Antimicrobial resistance in bacterial pathogens is a major impediment to successful therapy, and in sever- al instances, bacterial strains have arisen that are refracto- ry to most available antimicrobial treatments (1). (cdc.gov)
This in turn would help to better understand the development of resistance in pathogens and to prepare surveillance and control measures to reduce their dissemination. (biomedcentral.com)
The isolation of bacterial pathogens resistant to the commonly prescribed antibiotics from pregnant women symptomatic and asymptomatic for UTI calls for early screening of all pregnant women for UTI during antenatal care service delivery. (who.int)
Many factors hamper control of such infectious pathogens, including lack of vaccines, the presence of asymptomatic healthy carriers, existence of broad host range pathogens (with the hosts serving as reservoirs), resistance of pathogens to ordinary disinfectants, the long contamination-prone processes from production to consumption and increased frequency of resistance of pathogens to antibiotics. (researchsquare.com)
Streptococcus suis is one of the most important bacterial pathogens in the porcine industry and also a zoonotic agent. (biomedcentral.com)
Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields. (bvsalud.org)
The main mode of bacterial resistance in case of Gram-negative pathogens is mediated by the expression of enzymes able to hydrolyze this crucial ring: the β-lactamases [ 3 ]. (degruyter.com)
Hazelton BJ, Thomas LC, Unver T, Iredell JR. Rapid identification of Gram-positive pathogens and their resistance genes from positive blood culture broth using a multiplex tandem RT-PCR assay. (medscape.com)

Trends Microbiol 1995;3:136-40. (ignited.in)
Trends Microbiol 13: 20-26. (igem.org)

The actual origins of acquired resistance genes are unknown, but environmental microbes, including the strains producing antimicrobial agents, are believed to be important sources (4,5). (cdc.gov)
It is, thus, plausible that hospital wastewaters could provide opportunities to find novel carbapenemases and other resistance genes not yet described in clinical strains. (biomedcentral.com)
Here we sought to characterize conjugal horizontal gene transfer of plasmid-encoded fluoroquinolone resistance genes from two strains of Enterobacteriaceae, one clinical and one from a municipal wastewater treatment plant environment. (karger.com)
Escherichia coli strain LR09, containing a plasmid with the aac(6 ′ )-Ib-cr fluoroquinolone resistance gene, did not conjugate with any of the 15 strains tested, while Enterobacter aerogenes strain YS11 conjugated with two strains of E. coli . (karger.com)
Sixty-eight bacterial strains were isolated from 3 different microhabitats of V. gigantea and from 2 microhabitats of T. aeranthos bromeliad plants. (scielo.br)
Although vancomycin resistance in clinical strains of S. epidermidis or S. aureus has not been reported, vancomycin-resistant strains of S. haemolyticus have been isolated[21,22]. (cdc.gov)
WGS revealed that strains of each Salmonella serovar could be assigned to a single 7-gene MLST cluster, and strains within the clusters were identical strains and closely related as defined by the 0 and 10 cgSNPs and likely shared a common ancestor. (biomedcentral.com)
The fact that ST16 strains harbored more putative virulence genes and shared greater similarity with the genome of human serotype 2 strains suggests that they present a higher zoonotic and virulence potential than those from Canada and Spain. (biomedcentral.com)
More than 80% of the strains included in this study carried genes associated with resistance to tetracycline, lincosamides and macrolides. (biomedcentral.com)
In molecular biology, generalized transduction is routinely used as a technique to manipulate and construct bacterial strains. (bvsalud.org)
Antimicrobial susceptibility data revealed increasing resistance to co-trimoxazole and ampicillin, but strains remained highly susceptible to ofloxacin. (who.int)
Major and/or minor categorization (Sensitive S, Intermediate I and Resistance R) discrepancies were found at all the laboratories for either one or both reference strains. (bvsalud.org)

Resistance amongst Staphylococcus , Streptococcus , Neisseria gonorrhoeae , and members of the Enterobacteriaceae is now quite common. (toku-e.com)
This increase poses several problems, including a) the lack of available antimicrobials for therapy of infections caused by VRE, because most VRE are also resistant to multiple other drugs (e.g., aminoglycosides and ampicillin) previously used for the treatment of infections due to these organisms, and b) the possibility that the vancomycin resistance genes present in VRE may be transferred to other gram-positive microorganisms such as Staphylococcus aureus. (cdc.gov)
Tn5083 and Tn5084 are recombinants, and are comprised of fragments from several transposons including Tn5085, and a relative of a putative transposon from B. firmus (which contains similar genes to the cadmium resistance operon of Staphylococcus aureus), as well as others. (dadospdf.com)
Mammaliicoccus sciuri (formerly Staphylococcus sciuri) is the most probable evolutionary repository of the mecA gene, that later disseminated to S. aureus. (bvsalud.org)
Here we describe our studies on the genetic organization of phages infecting Staphylococcus aureus, a pathogen of human importance, by using an assembly of tools for gene annotation, identification of expression components, and phylogeny analysis. (bvsalud.org)
Staphylococcus , Corynebacterium , by dietary habits as well as by the ed, the number of frequent genes and Propionibacterium [8]. (who.int)

Some serovars of non-typhoidal Salmonella (NTS) have been associated with bloodstream infections and gastroenteritis, especially in children in Sub-Saharan Africa with circulating S. enterica serovars with drug resistance and virulence genes. (biomedcentral.com)
Virulence and antimicrobial resistance genes, sequence type, and cluster analysis were investigated using WGS data. (biomedcentral.com)
Between 100 and 118 virulence gene markers distributed across several Salmonella pathogenicity islands (SPIs), clusters, prophages, and plasmid operons were found in each isolate. (biomedcentral.com)

Microbial DNA was extracted from subgingival dental plaque (n=281) and V3-V4 regions of the 16S rRNA gene were sequenced to measure the relative abundances of 20 a priori - selected taxa with nitrate‐reducing capacity. (ahajournals.org)
The recent success gut microbial genes, obtained after has fantastic potential, it has only of faecal microbiota transplantation, sequencing whole faecal microbiota been about 10 years since the sci- especially in the context of Clostridi- metagenomes from 124 European indi- entific community first realized its im- um difficile infection, argues for such viduals [4]. (who.int)

2023. Geography Shapes the Genomics and Antimicrobial Resistance of Salmonella enterica Serovar Enteritidis Isolated from Humans. (fda.gov)
Nat Microbiol (2023). (janusinfo.se)

Antidepressant fluoxetine induces multiple antibiotics resistance in Escherichia coli via ROS-mediated mutagenesis Environment International,120: 421-430, 2018. (drfoxonehealth.com)
We demonstrate the utility of our new approach by applying it to identify genes used by Escherichia coli to survive in a wide range of different concentrations of the biocide Triclosan. (biorxiv.org)
Wang Y, Kim K. Effect of rpoSmutations on stress-resistance and invasion of brain microvascular endothelialcells in Escherichia coli K1. (ignited.in)

Allerberger F. Clin Microbiol Infect. (escmid.org)

Inactivation of antibiotics and the dissemination of resistance genes 1994 Science 264:375-382. (wikipedia.org)
Transcriptional modulation of bacterial gene expression by subinhibitory concentrations of antibiotics. (wikipedia.org)
These cells are embedded in extracellular polymeric substances, a matrix which is generally composed of eDNA, proteins and polysaccharides, showed high resistance to antibiotics. (biomedcentral.com)
Mycolic acids, a potential permeability barrier could be associated to the higher resistance to antibiotics [ 149 ]. (biomedcentral.com)
He shook his head, pointing to a pile of reports on his desk supporting my concerns about increasing bacterial resistance to antibiotics and told me that because of the political power of the pharmaceutical industry and livestock and poultry sectors, his hands were tied. (drfoxonehealth.com)
Some antidepressants, now widely prescribed, have been shown to increase bacterial resistance to antibiotics. (drfoxonehealth.com)
KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)

The enterosalivary nitrate‐nitrite‐nitric oxide pathway is an alternative pathway of nitric oxide generation, potentially linking the oral microbiome to insulin resistance and blood pressure (BP). (ahajournals.org)
Here, we profile the gut microbiome and chromatin characteristics in colon epithelium from mice fed either an obesogenic or control diet, followed by an analysis of the resultant changes in gene expression. (biomedcentral.com)
The obesogenic diet shapes the microbiome prior to the development of obesity, leading to altered bacterial metabolite production which predisposes the host to obesity. (biomedcentral.com)
Given the important roles of the gut microbiome in regulating host physiology and gene expression, a better understanding of the relationship between the host and the symbiont microbiota should provide new insights into human health and disease risk. (biomedcentral.com)

Appl Environ Microbiol 2005;71:5309-5317. (karger.com)
Environ Microbiol. (drfoxonehealth.com)

Int Microbiol 1999;2:169-76. (ignited.in)
Data of stool specimens yielding growth of non-O1 and non-O139 V. cholerae isolated at a national referral laboratory from 1999 to 2012 were retrospectively analysed and evaluated for resistance to ampicillin, tetracycline, chloramphenicol, co-trimoxazole and ofloxacin. (who.int)

Innate and induced resistance mechanisms of bacterial biofilms. (igem.org)
in Bacterial Biofilms (ed. (igem.org)

This increase poses important problems, including a) the lack of available antimicrobial therapy for VRE infections, because most VRE are also resistant to drugs previously used to treat such infections (e.g., aminoglycosides and ampicillin), and b) the possibility that the vancomycin-resistant genes present in VRE can be transferred to other gram-positive microorganisms (e.g. (cdc.gov)
Vancomycin resistance in enterococci has coincided with the increasing incidence of high-level enterococcal resistance to penicillin and aminoglycosides, thus presenting a challenge for physicians who treat patients who have infections caused by these microorganisms (1,4). (cdc.gov)
The emergence of antimicrobial resistance severely threatens our ability to treat bacterial infections. (frontiersin.org)
There are many approaches being used to control infections by suppressing its formation but CRISPR-CAS (gene editing technique) and photo dynamic therapy (PDT) are proposed to be used as therapeutic approaches to subside bacterial biofim infections, especially caused by deadly drug resistant bad bugs. (biomedcentral.com)
Polymicrobial infections involve both detrimental and beneficial interactions between different bacterial species. (biomedcentral.com)
ORIGINS (Oral Infections, Glucose Intolerance, and Insulin Resistance Study) enrolled 300 diabetes mellitus-free adults aged 20 to 55 years (mean=34±10 years) (78% women). (ahajournals.org)
The main therapeutic class involved against bacterial infections is based on the penicillin core: the β-lactams. (degruyter.com)
Bacterial skin infections--an observational study. (medscape.com)

September 20-22, 2022 - The Food and Drug Administration (FDA, the Agency, or we) will hold a virtual public meeting titled "2022 Public Meeting of the National Antimicrobial Resistance Monitoring System. (fda.gov)
2022. Advancing antimicrobial resistance monitoring in surface waters with metagenomic and quasimetagenomic methods. (fda.gov)

The discovery and tracking of antimicrobial resistance genes are essential for understanding the evolution of bacterial resistance and restraining its dispersion. (bvsalud.org)

Baucheron S, Tyler S, Boyd D, Mulvey MR, Chaslus-Dancla E, Cloeckaert A: AcrAB-TolC directs efflux-mediated multidrug resistance in Salmonella enterica serovar typhimurium DT104. (karger.com)
Tetracycline is also used in multidrug resistance studies and in cell culture applications as a selective agent. (toku-e.com)
It has been used in studies of multidrug resistance and potential side effects including acute pancreatitis. (toku-e.com)
Multidrug resistance was detected in 43.3% of Salmonella spp. (ophrp.org)
S . Enteritidis and S . Typhimurium presented the highest resistance (98.3%) and multidrug resistance rate (73.3%), respectively. (ophrp.org)

At the Institute Pasteur he established his molecular biology credentials by mapping the i and o genes of the lac operon. (wikipedia.org)
IglA is a 21 kDa protein encoded by a gene that is part of an iglABCD operon located on the Francisella pathogenicity island (FPI). (biomedcentral.com)
Other bacterial sRNAs, referred to as cis -encoded sRNAs, are present in close proximity to their targets, such as upstream, opposite of the 5′ UTR of the target, or between two genes in an operon [ 2 ]. (biomedcentral.com)

In eukaryotic cells, chromatin comprises a complex consisting of DNA, RNA, and proteins where covalent modifications correlate with regulation of gene expression [ 1 ]. (biomedcentral.com)

The vanA gene, which is frequently plasmid-borne and confers high-level resistance to vancomycin, can be transferred in vitro from enterococci to a variety of gram-positive microorganisms,[18,19] including S. aureus[20]. (cdc.gov)
Verhoef J, Mattsson E. The role ofcytokines in gram-positive bacterial shock. (ignited.in)

FEMS Microbiol Lett 2000;182:241-7. (ignited.in)

Resistance to tetracycline arises from loss of cell wall permeability, tetracycline efflux, ribosome protection and Tetracycline modification. (toku-e.com)
Acquired (as opposed to inherent) resistance has proliferated in many pathogenic organisms and greatly eroded the versatility of Tetracycline derivatives. (toku-e.com)
Tetracycline HCl is routinely used as a selective agent to select for bacterial cells that have been transformed with a plasmid that contains the tetracycline resistance gene, tet . (toku-e.com)

The actual increase in the incidence of VRE in U.S. hospitals might be greater than reported because the fully automated methods used in many clinical laboratories cannot consistently detect vancomycin resistance, especially moderate vancomycin resistance (as manifested in the VanB phenotype) (9-11). (cdc.gov)
Among the clinical populations of Gram-negative microorganisms, bla TEM-1 is the most frequently detected antimicrobial resistance gene and, although its expression results in penicillin resistance, diverse point mutations in the bla TEM-1 gene have contributed to the emergence of TEM-type extended-spectrum β-lactamases (ESBLs), resulting in simultaneous resistance to penicillins and broad-spectrum cephalosporins (8). (scielo.br)
Bacterial resistance to antimicrobials has become one of the greatest challenges for clinical microbiologists and healthcare practitioners worldwide. (karger.com)
bacteremia: clinical features, treatment outcomes, and clinical implication of antimicrobial resistance. (ac.ir)
In this case, we retrospectively tracked the bla(KPC) carbapenemase gene-bearing pKpQIL plasmid responsible for a CRE outbreak that occurred at the NIH Clinical Center in 2011. (nih.gov)
Data are lacking on the prevailing pneumococcal axone and levofloxacin was done fol owing the method serotypes in the Philippines, including their resistance to described by the Clinical and Laboratory Standard Insti- specific antimicrobials. (who.int)
Results: Seventy-seven (6.7%) bacterial species were isolated from 1153 clinical samples processed at the bacteriology unit of the hospital laboratory between June and December 2021, of which 65.0% (50/77) were members of the order Enterobacteriales. (bvsalud.org)

NDM (0.9% relative abundance to 16S rRNA genes) was the most common carbapenemase gene, followed by OXA-58 (0.84% relative abundance to 16S rRNA genes). (biomedcentral.com)
Among the investigated mobile genetic elements, class 1 integrons (11% relative abundance to 16S rRNA genes) were the most abundant. (biomedcentral.com)
Phylogenetic affiliations of mesopelagic acantharia and acantharian-like environmental 18S rRNA genes off the southern California coast. (mbl.edu)

Co-infection by different pathogenic bacterial species often increases the severity of the underlying condition, and are more difficult to treat [ 1 ]. (biomedcentral.com)

Here, we report a new strain with chromosomally integrated ethanologenic pathway under the control of a constitutive promoter, without recombinant plasmids and resistance genes. (biomedcentral.com)
Rapid methods for tracking plasmids carrying carbapenemase genes could greatly benefit infection control efforts. (nih.gov)
Sequence analysis demonstrates the presence of this gene in other bla(KPC) Tn4401-containing plasmids and suggests that this signature MS peak may be useful in tracking other plasmids conferring carbapenem resistance. (nih.gov)

We screened the Nebraska Transposon Mutant Library of 1920 single-gene inactivations in S. aureus strain JE2, for increased susceptibility to the anti-staphylococcal antimicrobials (ciprofloxacin, oxacillin, linezolid, fosfomycin, daptomycin, mupirocin, vancomycin, and gentamicin). (frontiersin.org)
The majority of the identified genes have not previously been associated with antimicrobial susceptibility in S. aureus . (frontiersin.org)
Our results demonstrate that many gene products contribute to the intrinsic antimicrobial resistance of S. aureus . (frontiersin.org)
Consistent with these in vitro findings, phage treatment did not reduce bacterial burdens in a neutropenic mouse S. aureus thigh infection model. (bvsalud.org)

Abstract - We have studied the mechanisms of the horizontal dissemination of a broad-spectrum mercury resistance determinant among Bacillus and related species. (dadospdf.com)

Antunes P, Novais C, Peixe L. Microbiol Spectr 2020;8(1). (escmid.org)

Impetigo Update: New Challenges in the Era of Methicillin Resistance. (medscape.com)

This report presents recommendations of the Hospital Infection Control Practices Advisory Committee for preventing and controlling the spread of vancomycin resistance, with a special focus on VRE. (cdc.gov)
How bacterial cyclases recognize phage infection is not known. (bvsalud.org)

The search for unknown mutations in genomic DNA is important for a broad spectrum of research studies, including fundamental research on gene structure and function, the study of genetic diseases and disorders, and species identification. (bio-rad.com)
We have shown that the mer determinant can either be located at the chromosome, or on a plasmid in the Bacillus species, and is carried by class II mercury resistance transposons: Tn5084 from B. cereus RC607 and B. cereus VKM684 (ATCC10702) and Tn5085 from Exiguobacterium sp. (dadospdf.com)
Antibiosis, which is defined by detrimental interactions between at least two species, usually involves the synthesis of antimicrobial molecules that inhibit growth or kill other bacterial species [ 18 ]. (biomedcentral.com)
The bacterial genus Exiguobacterium includes several species that inhabit environments with a wide range of temperature, salinity, and pH. (biomedcentral.com)
A catalogue of 178 reference bacterial birth, and the delivery type is the first core of species has been identified genomes distributed among different factor that has an impact. (who.int)

We conclude that PCR-based enrichment of transposon insertions in a TnSeq assay is reliable, but researchers interested in profiling putative essential genes should carefully weigh the number of amplification cycles employed in their library preparation protocols. (microbiologyresearch.org)

Antimicrobial Resistance Surveillance Program from 1 January 2012 to 31 December 2018. (who.int)

The study also demonstrates that the microbiota of hospital wastewater can serve as a reservoir of novel resistance genes, including previously uncharacterized carbapenemases with the potential to spread further. (biomedcentral.com)

are among the few microorganisms harboring different chromosomal β-lactamase genes, including cphA (also named imiH ), cepH and ampH , encoding class B, C and D β-lactamases, respectively (1). (scielo.br)

Gene products that confer intrinsic resistance to antimicrobial agents may be explored for alternative antimicrobial therapies, by potentiating the efficacy of existing antimicrobials. (frontiersin.org)
Two main mer determinant types have been described: narrow-spectrum mer determinants confer resistance to inorganic mercury salts only. (dadospdf.com)

Knowledge of these intrinsic resistance determinants provides alternative targets for compounds that may potentiate the efficacy of existing antimicrobial agents against this important pathogen. (frontiersin.org)
Intrinsic resistance to antimicrobials has traditionally been attributed to reduced permeability of the cell envelope, presence of inactivating enzymes or efflux pumps that can extrude the antimicrobial agents ( Cox and Wright, 2013 ). (frontiersin.org)

We conducted a factorially designed experiment where we passaged a bacterial host ( Bacillus subtilis ) and its phage (SPO1) in the presence versus absence of a seed bank consisting of dormant endospores. (nature.com)

High throughput sequencing of amplified integron gene cassettes identified a novel functional variant of an IMP-type (proposed IMP-81) carbapenemase gene (eight aa substitutions) along with recently described novel resistance genes like sul4 and bla RSA1 . (biomedcentral.com)

A recent refinement of this approach allows the roles of essential genes in conditional stress survival to be inferred by altering their expression. (biorxiv.org)

These mutations can be beneficial (or detrimental) depending on which gene they affect. (bio-rad.com)
After characterizing allelic variation with pooled population sequencing, we found that seed banks retained twice as many host genes with mutations, whether phages were present or not. (nature.com)
Reads mapping to the junctions are enumerated thus providing information on the number of transposon insertion mutations in each individual gene. (microbiologyresearch.org)

[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)

For example, instead of breeding for many years to move a disease resistance gene from a wild strawberry into a modern strawberry, gene editing allows a direct introduction of the genetic information. (ufl.edu)
By using CRISPR technology, these genes or gene variants can be moved into desirable genetic backgrounds that can be further moved via conventional crossing in later generations. (ufl.edu)

Frasson I, Cavallaro A, Bergo C, Richter SN, Palu G: Prevalence of aac(6 ′ )-Ib-cr plasmid-mediated and chromosome-encoded fluoroquinolone resistance in Enterobacteriaceae in Italy. (karger.com)

The sequence data showed evidence for recombination both between transposition genes and between mer determinants. (dadospdf.com)

Transposon mutagenesis has been proven to be a powerful tool to identify genes and networks which are involved in survival and fitness under a given condition by simultaneously assaying the fitness of millions of mutants, thereby relating genotype to phenotype and contributing to an understanding of bacterial cell biology. (biorxiv.org)
a software application for rapid large-scale comparative analysis of TraDIS experiments that predicts the impact of transposon insertions on nearby genes. (biorxiv.org)
Transposon-based approaches to identify essential bacterial genes. (microbiologyresearch.org)
Notably, the gene characterized here is located adjacent to the bla(KPC) Tn4401 transposon on the pKpQIL plasmid. (nih.gov)

A cluster and humans coming into contact with the animals (farm that includes three genes, vanH , vanA , and vanX , is required for high-level resistance to glycopeptides. (cdc.gov)
Food-to-Humans Bacterial Transmission. (escmid.org)
Barrier agains t pathogen s gut bacterial genes in the MetaHIT ation when attempting to extrapolate [10], although Proteobacteria, Ver- catalogue were also well represent- results obtained in mouse models to rucomicrobia, and Fusobacteria are ed in the other metagenomes that the situation in humans. (who.int)

This leads to accurate detection of both known and previously undescribed resistance genes in genomic and metagenomic sequence data. (biomedcentral.com)
The charge to the review committee was to provide recommendations regarding sampling strategies within a One Health paradigm, reporting antimicrobial sales and resistance data, and suitable ways to report whole genome sequence data and trends in the resistome. (fda.gov)

Detection of magA Gene in Klebsiella spp. (ac.ir)
However, the detection of a small subset of genes which had been previously described as essential is sensitive to the number of PCR cycles. (microbiologyresearch.org)
Suspect contamination if only one of several cultures is positive, if detection of bacterial growth is delayed (≥5 d), or if multiple organisms are isolated from one culture. (medscape.com)

Anatomy6

Organisms54

Diseases9

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes83

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture9

Information Science4

Health Care8

Antibiotic43

Bacteria24

Isolates19

Pathogens8

Trends Microbiol2

Strains12

Staphylococcus6

Virulence3

Microbial2

20232

Escherichia3

Clin Microbiol Infect1

Antibiotics7

Microbiome4

Environ Microbiol2

19992

Biofilms2

Infections8

20222

Tracking of antimicrobial resistance1

Multidrug resistance5

Operon3

Proteins1

Gram-positive2

20001

Tetracycline3

Clinical7

RRNA genes3

Pathogenic bacterial1

Plasmids3

Aureus4

Horizontal1

Spectr1

Methicillin resistance1

Infection2

Species5

Putative1

20181

Microbiota1

Microorganisms1

Confer2

Intrinsic2

Bacillus1

Carbapenemase1

Essential genes1

Mutations3

Phenotypic1

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Enterobacteriaceae1

Determinants1

Transposon4

Humans3

Sequence2

Detection3