• The genes coding for it are referred to as 16S rRNA genes and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. (wikipedia.org)
  • For bacteria, the assays target the 16S rRNA gene, and for fungi, they target ribosomal RNA genes. (qiagen.com)
  • With 100-fold the number of genes in the human genome 1 , this microbial collection is a rich genetic signature of clinical significance that we have only recently gained the tools to explore. (ddw-online.com)
  • 7) The human gut alone contains on average: 40,000 bacterial species, 8) 9 million unique bacterial genes and 100 trillion microbial cells. (mpkb.org)
  • The most commonly used molecular approach for exploring microbial diversity is based on the PCR amplification and sequencing of genes encoding small subunit ribosomal RNA (16S rRNA) directly from environmental sources [ 16 ]. (biomedcentral.com)
  • We sequenced 16S ribosomal RNA genes from 179 endoscopic lavage samples from different intestinal regions in 64 subjects (32 controls, 16 CD and 16 UC patients in clinical remission). (escholarship.org)
  • IBM plans to find out more by analyzing millions of microbial genes, starting with those belonging to gut bacteria. (longevityfacts.com)
  • Multiple sequences of the 16S rRNA gene can exist within a single bacterium. (wikipedia.org)
  • It is suggested that 16S rRNA gene can be used as a reliable molecular clock because 16S rRNA sequences from distantly related bacterial lineages are shown to have similar functionalities. (wikipedia.org)
  • In addition to highly conserved primer binding sites, 16S rRNA gene sequences contain hypervariable regions that can provide species-specific signature sequences useful for identification of bacteria. (wikipedia.org)
  • With third-generation sequencing coming to many labs, simultaneous identification of thousands of 16S rRNA sequences is possible within hours, allowing metagenomic studies, for example of gut flora. (wikipedia.org)
  • Additionally, the 16S gene contains highly conserved sequences between hypervariable regions, enabling the design of universal primers that can reliably produce the same sections of the 16S sequence across different taxa. (wikipedia.org)
  • Consequently, established primers specifically amplifying the archaeal 16S ribosomal gene region are scarce compared to the variety of primers targeting bacterial sequences. (frontiersin.org)
  • These research applications include ultra deep sequencing for detection of low-frequency somatic mutations in cancer samples, discovery of rare variants in HIV infected individuals, analysis of exon sequences of disease-associated regions, identified by whole genome association studies and sequencing of 16S ribosomal RNA regions to characterize the microbial abundance and diversity in metagenomic samples. (pressebox.com)
  • The 16S rRNA sequences of the four archaeal bands were closely associated with Methanosaeta concilii and Methanobacterium formicum. (hku.hk)
  • A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
  • Assigning amplicon sequences to operational taxonomic units (OTUs) is an important step in characterizing microbial communities across large data sets. (nih.gov)
  • Efficient and robust software tools are needed to assign microbial sequences into taxonomic groups for characterization and comparison of communities. (nih.gov)
  • Prediction of taxonomy for marker gene sequences such as 16S ribosomal RNA (rRNA) is a fundamental task in microbiology. (peerj.com)
  • Using QIIME to analyze 16S rRNA gene sequences from microbial communities. (nau.edu)
  • In recent years, culture-independent, phylogenetic characterization of microbial communities using 16S ribosomal RNA (16S rRNA) gene sequences directly extracted from biological samples has provided a broad understanding of vaginal microbial communities among reproductive age women [ 1 ]. (biomedcentral.com)
  • At these sites, we detected a 60% shift in the microbial community composition compared with reference sites, mostly related to increases in Chloroflexi sequences. (uni-bielefeld.de)
  • In contrast to metagenomic detection of unknown pathogens which sequences all available genetic material, 16S sequencing specifically targets the small ribosomal subunit. (folkhalsomyndigheten.se)
  • As they reported in a paper published in Cell Host & Microbe on Thursday, Haller and his colleagues used high-throughput 16S ribosomal RNA gene sequencing to profile gut microbial community composition in fecal samples from 1,976 individuals from Germany enrolled in the prospective KORA population study, detecting distinct levels of specific bugs across the day in individuals with available time of defecation data. (genomeweb.com)
  • Using 16S ribosomal RNA gene amplicon sequencing and metagenomics, we resolved the microbial community composition on the North and South Shelves of Iceland, analyzed its evolution from 2011 to 2018, identified frequently occurring taxa, and predicted their potential metabolism. (matis.is)
  • Community similarity correlated significantly with differences in chlorophyll a content and with the presence of upward porewater fluxes carrying reduced compounds (e.g., ammonia and sulfide), suggesting that trophic resource availability is a main driver for changes in microbial community composition. (mpg.de)
  • Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. (uidaho.edu)
  • We then compared gut microbial composition using 16S ribosomal RNA gene sequencing in fecal samples to detect species with differential abundance between 2 groups. (researchsquare.com)
  • However, the high complexity and low inter-individual overlap of intestinal microbial composition are formidable barriers to identifying microbial taxa representing this dysbiosis. (escholarship.org)
  • These difficulties might be overcome by an ecologic analytic strategy to identify modules of interacting bacteria (rather than individual bacteria) as quantitative reproducible features of microbial composition in normal and IBD mucosa. (escholarship.org)
  • CD and UC patients showed a reduction in phylogenetic diversity and shifts in microbial composition, comparable to previous studies using conventional mucosal biopsies. (escholarship.org)
  • High throughput microbial 16S ribosomal RNA gene sequencing indicated that diversity, richness and composition of howler GI microbiomes varied with host habitat in relation to diet. (illinois.edu)
  • Stool samples were collected for 16S ribosomal RNA gene sequencing and microbial composition analysis in IBS subjects. (bvsalud.org)
  • However, data are not currently available regarding the representation of microbial species composition in patient-collected swabs compared to provider-collected swabs among pregnant women. (biomedcentral.com)
  • pH was among the factors significantly, yet not mainly, explaining changes in microbial community composition. (uni-bielefeld.de)
  • AIM: To determine the efficacy of a low FODMAP diet in childhood IBS and whether gut microbial composition and/or metabolic capacity are associated with its efficacy. (medscape.com)
  • Baseline gut microbial composition (16S rRNA sequencing) and metabolic capacity (PICRUSt) were determined. (medscape.com)
  • The Microbial Genomics and Metagenomics Laboratory (MGML) provides metagenomic sequencing and analysis support to the Cincinnati Children's, University of Cincinnati and surrounding communities. (cincinnatichildrens.org)
  • The MGML is staffed and equipped to provide services ranging from 16S ribosomal RNA sequencing (16S rRNA), shotgun metagenomics and RNA sequencing. (cincinnatichildrens.org)
  • Specific applications include de novo sequencing and re-sequencing of genomes, metagenomics, RNA analysis, and targeted sequencing of DNA regions of interest. (pressebox.com)
  • Such diurnal gut microbiome fluctuations may have been largely missed in prior gut microbiome studies that did not track stool sampling time, the team suggested, potentially contributing to the inter-individual and regional variability documented in gut microbial communities. (genomeweb.com)
  • human gut microbiome, data repositories, large-scale and integrative computational tools, modelling, immunomodulation, drug screening Background The human gastrointestinal tract is a complex ecosystem in which eukaryotic cells continuously interact with nutrients and with the complex microbial population of the gut microbiota [1]. (deepdyve.com)
  • As we continue to learn about our microbial communities, the landscape of next generation technologies aiming to progress this understanding and employ it for the manipulation of our microbiome has become increasingly competitive. (ddw-online.com)
  • In this study, we completed a longitudinal 16S ribosomal RNA survey of the lung microbiome on replicate sputum samples collected from 4 former smokers with COPD (Stage 2) within a 2-day time period. (cdc.gov)
  • To assess whether Mtb infection modifies the lung microbiome, and identify changes in microbial abundance and diversity as a function of pulmonary inflammation, we compared infected and uninfected lung lobe washes collected serially from 26 macaques by bronchoalveolar lavage over the course of infection. (biomedcentral.com)
  • To conclude, dietary n-3 LCFA had a positive impact on the microbiome of suckling piglet's feces by increasing microbial diversity and some beneficial bacteria populations, had a few minor modifications on the microbiome of milk and gestating sows' feces and did not change the microbiome in lactating sows' feces or colostrum. (irta.cat)
  • Human Microbiome Project researchers have made IBM's job easier by creating a reference database of normal microbial variation in humans. (longevityfacts.com)
  • The research team calculated that more than 10,000 microbial species occupy the human microbiome and also identified 81 to 99% of the genera. (longevityfacts.com)
  • Host microbial community assemblage will be characterised using high throughput 16S ribosomal RNA sequencing of collected faecal samples. (cyrilgrueter.net)
  • The laboratory procedure is based on amplifying and sequencing four variable regions of the 16S rRNA gene with two amplicons. (folkhalsomyndigheten.se)
  • The Illumina MiSeq sequencing of 16S rRNA gene amplicons revealed that genus Flavobacterium was the predominant group that preferentially accumulated in Allium rhizospheres. (nature.com)
  • New gene-based technologies can now be employed to examine microbial diversity through the use of small sub-unit ribosomal DNA analysis (e.g. 16S rDNA) and to understand the function of complex microbial ecosystems in the rumen through metagenomic analysis. (nhbs.com)
  • Microbial NGS methods can range from ribosomal RNA analysis (16S rRNA sequencing), to parallel genomic sequencing or shotgun metagenomic sequencing, to whole genome sequencing, to metatranscriptomics. (ddw-online.com)
  • The 16S rRNA gene is used for phylogenetic studies as it is highly conserved between different species of bacteria and archaea. (wikipedia.org)
  • Although it was originally used to identify bacteria, 16S sequencing was subsequently found to be capable of reclassifying bacteria into completely new species, or even genera. (wikipedia.org)
  • While 16S hypervariable regions can vary dramatically between bacteria, the 16S gene as a whole maintains greater length homogeneity than its eukaryotic counterpart (18S ribosomal RNA), which can make alignments easier. (wikipedia.org)
  • In contrast to Bacteria, the archaeal domain was often not particularly addressed in the analysis of microbial communities. (frontiersin.org)
  • Here, we review the literature on culture-independent analysis of microbial diversity in the BE and explore what it tells us about the dynamics and distribution of bacteria and fungi in these environments. (biomedcentral.com)
  • Its overall accuracy is 93.6% when compared to the accuracy of 16S Ribosomal RNA gene sequencing, the gold standard for identifying anaerobic bacteria, Enterobacteriaceae, Gram-positive aerobes, fastidious Gram-negative bacteria, Gram-negative non-Enterobacteriaceae, and yeast. (aacc.org)
  • Ecology, Microbial population biology, Bacteria, Microbiology and Genetics are his primary areas of study. (research.com)
  • This book presents a comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques that are currently in use for studying the gut microbial ecology of ruminants. (nhbs.com)
  • This manual will `demystify' the methods in molecular microbial ecology for readers who are novice in the field but are excited by the prospects of the technology. (nhbs.com)
  • The authors used the now-familiar molecular techniques of microbial ecology (16S ribosomal RNA (rRNA) gene amplicon sequencing) to characterise bacterial communities in sputum and bronchoalveolar lavage fluid collected from a large and well-matched cohort of patients with non-cystic fibrosis bronchiectasis. (ersjournals.com)
  • It is an acronym for Quantitative Insights Into Microbial Ecology, and has been used to analyze and interpret nucleic acid sequence data from fungal, viral, bacterial, and archaeal communities. (nau.edu)
  • Microbial modules thus provide an integrative view of microbial ecology relevant to IBD. (escholarship.org)
  • His studies deal with areas such as Temperature gradient gel electrophoresis, Hyporheic zone, Soil water, Microbial ecology and Environmental chemistry as well as Microbial population biology. (research.com)
  • William E. Holben spends much of his time researching Microbial population biology, Ecology, Fractionation, Greenland ice sheet and Microbial biodegradation. (research.com)
  • His Ecology research includes elements of Microbial ecology and Stable-isotope probing. (research.com)
  • Microbial population biology, Ecology, Isolation, Bacterial dna and Microbiology are his primary areas of study. (research.com)
  • His work deals with themes such as Microbial ecology and Stable-isotope probing, which intersect with Ecology. (research.com)
  • New genomic based methods such as polymerase chain reaction ( PCR ) detect bacterial forms based on the presence of bacterial DNA or RNA. (mpkb.org)
  • The use of molecular techniques such as 16S rRNA gene sequencing and polymerase chain reaction (PCR) have identified difficult-to-culture organisms and expanded knowledge of the microflora associated with dental abscess. (medscape.com)
  • The bacterial 16S gene contains nine hypervariable regions (V1-V9), ranging from about 30 to 100 base pairs long, that are involved in the secondary structure of the small ribosomal subunit. (wikipedia.org)
  • 16S ribosomal RNA (or 16S rRNA) is the RNA component of the 30S subunit of a prokaryotic ribosome (SSU rRNA). (wikipedia.org)
  • Three archaeal 16S primer pairs as well as two bacterial and one general microbial 16S primer pairs were comprehensively tested by in-silico evaluation and performing an experimental analysis of a complex microbial community of a biogas reactor. (frontiersin.org)
  • While the entire 16S sequence allows for comparison of all hypervariable regions, at approximately 1,500 base pairs long it can be prohibitively expensive for studies seeking to identify or characterize diverse bacterial communities. (wikipedia.org)
  • Therefore, we hypothesized that rhizospheres of Allium plants harbor unique microbial communities and that some of the predominant microorganisms are involved in the suppression of Fusarium wilt induced by Allium cultivation. (nature.com)
  • A molecular phylogenetic survey of sea-ice microbial communities (SIMCO). (nih.gov)
  • In this study, we conducted annual sampling at four hydrographic stations over several years to characterize marine microbial communities and their metabolic potential. (matis.is)
  • Genome Insider: Methane Makers in Yosemite's Lakes Meet researchers who sampled the microbial communities living in the mountaintop lakes of the Sierra Nevada mountains to see how climate change affects freshwater ecosystems, and how those ecosystems work. (doe.gov)
  • OptiFit allows researchers to compare microbial communities across different studies or add new data to existing studies without sacrificing the quality of the group assignments. (nih.gov)
  • Our results show significant differences in microbial communities between stations outside and inside the SWIR, which were mostly explained by environmental selection. (mpg.de)
  • At the stations in the SWIR axial valley (3,655-4,448 m water depth), microbial communities were enriched in bacterial and archaeal taxa common in organic matter-rich subsurface sediments (e.g. (mpg.de)
  • We conclude that ultraslow spreading ridges create a unique environmental setting in sedimented segments without distinct hydrothermal activity, and play an important role in shaping microbial communities and promoting diversity, but also in connectivity among deep-sea habitats. (mpg.de)
  • The following protocols describe how to install QIIME on a single computer and use it to analyze microbial 16S sequence data from nine distinct microbial communities. (nau.edu)
  • Our analysis showed that increases in soil salinity correlated with significant differences in the alpha and beta diversity of the microbial communities across saline and non-saline soil samples. (unboundmedicine.com)
  • Thus, chimpanzees provide a good model to explore the mechanisms by which habitat fragmentation may influence host gut microbial communities. (cyrilgrueter.net)
  • To understand how ocean acidification (OA) influences sediment microbial communities, naturally CO2-rich sites are increasingly being used as OA analogues. (uni-bielefeld.de)
  • Here, we used an extensive array of sediment and bottom water parameters to evaluate pH effects on sediment microbial communities at hydrothermal CO2 seeps in Papua New Guinea. (uni-bielefeld.de)
  • Thus, we recommend an ecosystem approach when assessing OA effects on sediment microbial communities under natural conditions. (uni-bielefeld.de)
  • Hassenrück C, Fink A, Lichtschlag A, Tegetmeyer H, de Beer D, Ramette A. Quantification of the effects of ocean acidification on sediment microbial communities in the environment: the importance of ecosystem approaches. (uni-bielefeld.de)
  • The epithelium function and the mucosal associated immune system are influenced by direct host-microbiota interactions and through modulation of the microbial metabolism [7]. (deepdyve.com)
  • Research increasingly demonstrates that within each discrete microbial environment (e.g., the skin, gut, vaginal flora), unique compositions of microbiota correspond to various healthy and diseased states. (ddw-online.com)
  • In addition to precise target quantification, a 5-plex dPCR run also shows highly specific detection of different microbial water pathogens (see figure Water microbial pathogen detection in 5-plex on QIAcuity ). (qiagen.com)
  • How specific pathogens modify and influence host microbial landscapes and site-specific immunity, particularly in the lung, is poorly understood. (biomedcentral.com)
  • Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates. (cdc.gov)
  • Many community studies select semi-conserved hypervariable regions like the V4 for this reason, as it can provide resolution at the phylum level as accurately as the full 16S gene. (wikipedia.org)
  • Han XY , Pham AS , Tarrand JJ , Sood PK , Luthra R . Rapid and accurate identification of mycobacteria by sequencing hypervariable regions of the 16S ribosomal RNA gene. (cdc.gov)
  • The microbial community structure of granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating brewery effluent was studied by denaturing gradient gel electrophoresis (DGGE). (hku.hk)
  • Each assay is based on an endpoint PCR amplification of a species-specific genetic region of the relevant microbe or a region of an individual microbial gene. (qiagen.com)
  • The 16S ribosomal RNA (16S rRNA) gene is present in most bacterial species. (technologynetworks.com)
  • This study set a baseline for the marine microbial diversity in Icelandic marine waters and identified three photosynthetic microorganisms - the cyanobacteria Synechococcus and two members of the Chlorophyta clade - as valuable indicator species for future monitoring, as well as for application in ecosystem modeling in context with research on climate change. (matis.is)
  • 16S sequencing is performed to determine which bacterial species are present in a clinical sample or isolate. (folkhalsomyndigheten.se)
  • Our findings suggest that Mtb can alter the microbial landscape of infected lung lobes and that these interactions induce dysbiosis that can disrupt oral-airway boundaries, shift overall lung diversity, and modulate specific microbial relationships. (biomedcentral.com)
  • The highly preserved microbial modules accurately classified IBD status of individual patients during disease quiescence, suggesting that microbial dysbiosis in IBD may be an underlying disorder independent of disease activity. (escholarship.org)
  • Considering the maternal physiologic changes in pregnancy, including the gravid abdomen and increased physiologic vaginal discharge, we aimed to evaluate if patient-collected swabs of the mid-vagina in pregnancy reflect the same microbial community characteristics as provider-collected swabs obtained during speculum exam. (biomedcentral.com)
  • Some thermophilic archaea (e.g. order Thermoproteales) contain 16S rRNA gene introns that are located in highly conserved regions and can impact the annealing of "universal" primers. (wikipedia.org)
  • The 16S rRNA gene is highly conserved with certain variable regions. (folkhalsomyndigheten.se)
  • The application of next-generation sequencing technology in microbial community analysis increased our knowledge and understanding of the complexity and diversity of a variety of ecosystems. (frontiersin.org)
  • 16S rRNA gene data derived from a shotgun metagenome of the same reactor sample added an additional perspective on the community structure. (frontiersin.org)
  • Overall our results indicate a rather limited comparability between community structures investigated and determined using different primer pairs as well as between metagenome and 16S rRNA gene amplicon based community structure analysis. (frontiersin.org)
  • The complexity of the lung microbial community in COPD patients has been highlighted in recent years. (cdc.gov)
  • The results showed correlations between the marine microbial community profiles and the water masses in spring, between the North and South Shelves of Iceland. (matis.is)
  • QIIME (canonically pronounced "chime") is a software application that performs microbial community analysis. (nau.edu)
  • On an individual macaque level, we found significant heterogeneity in both the magnitude and duration of change within the lung microbial community that was unrelated to lung inflammation and lobe involvement as seen by positron emission tomography/computed tomography (PET/CT) imaging. (biomedcentral.com)
  • By comparing microbial interaction networks pre- and post-infection using the predictive algorithm SPIEC-EASI, we observe that extra connections are gained by Actinomycetales , the order containing Mtb, in spite of an overall reduction in the number of interactions of the whole community post-infection, implicating Mtb-driven ecological reorganization within the lung. (biomedcentral.com)
  • 2004. Analysis and comparison of the microbial community structures of two enrichment cultures capable of reductively dechlorinating TCE and cis-DCE. (nih.gov)
  • Effects of enrichment with salicylate on bacterial selection and PAH mineralization in a microbial community from a bioreactor treating contaminated soil. (nih.gov)
  • His study focuses on the intersection of Microbial population biology and fields such as Clostridium with connections in the field of Community structure. (research.com)
  • Processes such as host-microbe crosstalk, immune activation and inflammation, microbe-microbe signalling, microbial metabolism and antimicrobial activity are bioactive in the human gut [3]. (deepdyve.com)
  • Some commentators have gone so far as to refer to the human body as a superorganism whose "whose metabolism represents an amalgamation of microbial and human attributes. (mpkb.org)
  • Our dPCR Microbial DNA Detection Assays can be combined for multiplex analysis. (qiagen.com)
  • A comparison between singleplex and multiplex analysis using the dPCR Microbial DNA Detection Assays shows similar and highly precise quantification for all targets (see figure Microbial detection in multiplex dPCR on QIAcuity ). (qiagen.com)
  • Soil microbiomes analysis using pyrosequencing of 16S rRNA gene indicated that Actinobacteria were dominant in saline soil samples whereas Proteobacteria predominated in non-saline soil samples. (unboundmedicine.com)
  • Analysis of weighted co-occurrence network revealed 5 microbial modules. (escholarship.org)
  • CloVR-Comparative: automated, cloud-enabled comparative microbial genome sequence analysis pipeline. (cdc.gov)
  • This 'alpha diversity', or microbial diversity within a sample, can include taxonomic assignment. (ddw-online.com)
  • cellular organisms 16S ribosomal RNA gene, partial sequence. (nih.gov)
  • NEW YORK - A team led by researchers in Germany has identified gut microbial oscillations over the span of a single day that appear to be disrupted in individuals with type 2 diabetes (T2D) and other metabolic conditions. (genomeweb.com)
  • Furthermore, researchers examine 'beta diversity' (microbial diversity between samples) to establish the significance of an individual's microbial profile across population-wide references. (ddw-online.com)
  • The researchers analyzed the human and microbial DNA with DNA sequencing machines. (longevityfacts.com)
  • Moreover, it is well recognized that the excessive use of broad-spectrum antibiotics can affect the relative proportions of gut microbial populations and foster bacterial resistance [24]. (deepdyve.com)
  • Given the positive relationship between host gut microbial diversity and overall health, this research may provide further support that conservation efforts that reduce fragmentation of chimpanzee habitats may be among the most effective at maintaining healthy and viable populations of this endangered, iconic primate. (cyrilgrueter.net)
  • This approach readily determines several orders of magnitude more microbial diversity than culture-based methods, often discovering novel organisms with no known growth conditions, and has dramatically altered our understanding of global microbial diversity [ 14 ]. (biomedcentral.com)
  • The differences in marine microbial diversity appear to be linked to the average seawater temperature in the mixed surface layer at each sampling station which also constrains the relative abundance of photosynthetic microorganisms. (matis.is)
  • Moisture response functions for soil microbial carbon (C) mineralization remain a critical uncertainty for predicting ecosystem-climate feedbacks. (org.in)
  • The Microbiology study combines topics in areas such as Sphingomonas paucimobilis, 16S ribosomal RNA, Soil microbiology and Homology. (research.com)
  • Using lung tissue from 33 controls and 143 cancer cases, we conduct 16S ribosomal RNA (rRNA) bacterial gene sequencing, with RNA-sequencing data from lung cancer cases in The Cancer Genome Atlas serving as the validation cohort. (biomedcentral.com)
  • Global epidemiology of non-influenza RNA respiratory viruses: data gaps and a growing need for surveillance. (cdc.gov)
  • The basis of the GENSAM project is the joint national management of clinical microbial sequencing data. (folkhalsomyndigheten.se)
  • Their impact on the diversity and connectivity of benthic deep-sea microbial assemblages is poorly understood, especially for hydrothermally inactive, magma-starved ridges. (mpg.de)
  • For the dPCR reaction, the assay is combined with the QIAcuity Probe PCR Kit (DNA targets) or the QIAcuity OneStep Advanced Probe Kit (RNA targets). (qiagen.com)
  • Multiplex dPCR-based detection of microbial targets offers a flexible setup of small panels in fewer reactions, thus conserving precious samples. (qiagen.com)
  • This application note describes a comprehensive, efficient workflow to sequence 16S rRNA variable regions. (technologynetworks.com)
  • Carl Woese and George E. Fox were two of the people who pioneered the use of 16S rRNA in phylogenetics in 1977. (wikipedia.org)
  • Carl Woese pioneered this use of 16S rRNA in 1977. (wikipedia.org)
  • As a result, 16S rRNA gene sequencing has become prevalent in medical microbiology as a rapid and cheap alternative to phenotypic methods of bacterial identification. (wikipedia.org)
  • Until recently, knowledge of ruminant gut microbiology was primarily obtained using classical culture based techniques, which probably only account for 10 to 20% of the rumen microbial population. (nhbs.com)
  • His Microbiology research is multidisciplinary, relying on both 16S ribosomal RNA and Microbial population biology. (research.com)
  • His Microbial population biology research incorporates elements of Isolation, Microbiology, Environmental chemistry, Carbon dioxide and Bacterial dna. (research.com)
  • Furthermore, in-silico evaluation of primers, especially those for amplification of archaeal 16S rRNA gene regions, does not necessarily reflect the results obtained in experimental approaches. (frontiersin.org)
  • The by far most often used marker for prokaryotic diversity studies is the 16S rRNA or its corresponding gene. (frontiersin.org)