• Megakaryocytes (MKs) generate platelets via intravascular protrusions termed proplatelets, which are tandem arrays of platelet-sized swellings with a beaded appearance. (nih.gov)
  • The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. (nih.gov)
  • In stark contrast, deletion of the prosurvival protein Bcl-xL resulted in megakaryocyte apoptosis and a failure of platelet shedding. (medhelp.org)
  • Mouse prenatal platelet-forming lineages share a core transcriptional program but divergent dependence on MPL. (edu.au)
  • http://dx.doi.org/10.1083/jcb.201410052 ) show that platelet release from megakaryocytes can be induced by interleukin-1α (IL-1α) via a new rupture mechanism, which yields higher platelet numbers, occurs independently of the key regulator of megakaryopoiesis thrombopoietin, and may occur during situations of acute platelet need. (rupress.org)
  • This work provides for the first time an explanation of how megakaryocytes can maintain platelet mass equilibrium and quickly restore platelet numbers under pathological conditions associated with increased platelet turnover. (rupress.org)
  • regulate megakaryocyte maturation, platelet activation, and transcript profile during inflammation in mice and humans. (umassmed.edu)
  • Platelet and Megakaryocyte Roles in Innate and Adaptive Immunity. (umassmed.edu)
  • HPS patients and mouse models all suffer from excessive bleeding and bruising due to defects in the formation of platelet dense granules, but little is known about the dense granule membrane or how and when it forms. (chop.edu)
  • Altered bioavailability of platelet-derived factor VIII during thrombocytosis reverses phenotypic efficacy in haemophilic mice. (ny.gov)
  • CLEC-2 is highly expressed on resting platelets and megakaryocytes and at a lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. (biolegend.com)
  • ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cellto cell junctions. (reliatech.de)
  • ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell to cell junctions. (reliatech.de)
  • With other collaborators, the lab has extended its analyses of LRO biogenesis to dense and alpha granules in platelets and megakaryocytes, lamellar bodies in lung epithelial type II cells, and phagosomes in dendritic cells. (chop.edu)
  • flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes. (topsan.org)
  • The mouse monoclonal antibody CB38 (NL07) recognizes an extracellular epitope of CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. (exbio.cz)
  • Even though the platelets released by megakaryocyte rupture were mildly enlarged in size, they were functionally indistinguishable from proplatelet-derived platelets. (rupress.org)
  • For example, whereas melanosome biogenesis in skin melanocytes is constitutive, dense granule biogenesis is limited to a late stage of megakaryocyte differentiation into proplatelet strings. (chop.edu)
  • Conversely, a range of pathophysiological insults, including chemotherapy, are thought to cause thrombocytopenia by inducing the apoptotic death of megakaryocytes and their progenitors. (medhelp.org)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. (rndsystems.com)
  • Although much about the pathophysiology of (secondary) bone marrow fibrosis remains to be elucidated, it is known that cytokines from bone marrow macrophages, megakaryocytes, and platelets appear to be necessary for fibrosis to occur. (nih.gov)
  • Here, we visualised the sequential phases of intravascular MK protrusions and fragments in living mouse bone marrow (BM), using intravital microscopy, and examined their ultrastructure. (nih.gov)
  • Bone marrow megakaryocytes engulf neutrophils in a phenomenon termed emperipolesis. (elifesciences.org)
  • Increased expression of inflammatory cytokines, lysyl oxidase, transforming growth factor-β, impaired megakaryocyte function, and aberrant JAK-STAT signaling have all been implicated in the pathogenesis of bone marrow fibrosis. (haematologica.org)
  • During these investigations, researchers discovered that mouse models infected with the novel coronavirus lost approximately 25% of their bone mass within two weeks of contagion. (iu.edu)
  • They also found mouse models with a 63% increase in osteoclasts, the cells that cause bone to break down. (iu.edu)
  • Platelets are continuously produced by fragmentation of the cytoplasm of their giant polyploid precursors in the bone marrow, the megakaryocytes. (rupress.org)
  • The diagnostic term "myelofibrosis" should not be used to describe fibrotic lesions of the bone marrow in mice and rats. (nih.gov)
  • A bone marrow biopsy obtained during that hospitalization showed adequate megakaryocytes, mild megaloblastic changes in erythroid precursors, and adequate iron stores. (cdc.gov)
  • In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant mouse IL-11 is observed. (rndsystems.com)
  • In vivo, mice with a Bak−/− Bax−/− hematopoietic system were protected against thrombocytopenia induced by the chemotherapeutic agent carboplatin. (medhelp.org)
  • We found that HSCs from Lnk-/- mice have an increased quiescent fraction, decelerated cell cycle kinetics, and enhanced resistance to repeat treatments with cytoablative 5-fluorouracil in vivo compared with WT HSCs. (jci.org)
  • In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
  • The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
  • Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
  • These findings identify emperipolesis as a new cell-in-cell interaction that enables neutrophils and potentially other cells passing through the megakaryocyte cytoplasm to modulate the production and membrane content of platelets. (elifesciences.org)
  • RBL-2H3 cells stably expressing HA-tagged mouse CLEC2. (biolegend.com)
  • Histologic assessment of patients with systemic sclerosis-associated PAH and the hypoxia/SU5416 mouse model identified the presence von Willebrand factor/α-smooth muscle actin-positive endothelial cells in up to 5% of pulmonary vessels. (surrey.ac.uk)
  • MegaCult™-C Collagen and Medium with Lipids kit includes medium and collagen solution for colony-forming unit (CFU) assays of human or mouse megakaryocyte progenitor cells (CFU-Mk). (stemcell.com)
  • We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
  • Immunofluorescence staining of CD31 (red) and ESAM (green) in a mixture of primary human dermal lymphatic endothelial cells (HDLEC) and the mouse myoblast cell line C2C12 with monoclonal mouse anti-human CD31 #WM-59 [Cat# 101-M92] and polyclonal rabbit anti-human ESAM [Cat# 102-PA42AG]. (reliatech.de)
  • Additionally, the lab has identified defects in innate immunity to bacterial infection from a mouse HPS2 model, partly explaining the recurrent bacterial infections observed in HPS2 patients, and is uncovering mechanisms by which the endosomal adaptor AP-3 influences toll-like receptor recruitment to phagosomes, inflammasome signaling from phagosomes, and autophagy activation in dendritic cells. (chop.edu)
  • Expression of protease activated receptor 3 (PAR3) is upregulated by induction of megakaryocyte phenotype in human erythroleukemia (HEL) cells. (ny.gov)
  • Researchers at the Center for iPS Cell Research and Application at Kyoto University in Japan presented data at a meeting of the American Society of Hematology showing they were able to create the cells in the laboratory and confirm they had the same life span as normal human platelets when infused in mice. (ibtimes.com)
  • The Japanese researchers set out to create an immortalized cell line with a large number of high-quality megakaryocytes -- precursor cells that develop into platelets -- from stem cells that can be grown indefinitely and differentiate into a variety of cell types in the body. (ibtimes.com)
  • Tethered neutrophils enter in membrane-bound vesicles before penetrating into the megakaryocyte cytoplasm. (elifesciences.org)
  • Circulating platelets were thought to arise solely from the protrusion and fragmentation of megakaryocyte cytoplasm. (rupress.org)
  • Intracytoplasmic neutrophils develop membrane contiguity with the demarcation membrane system, thereby transferring membrane to the megakaryocyte and to daughter platelets. (elifesciences.org)
  • Transit through megakaryocytes can be completed as rapidly as minutes, after which neutrophils egress intact. (elifesciences.org)
  • In addition to its role in megakaryocyte production, signaling initiated by thrombopoietin (TPO) activation of its receptor, myeloproliferative leukemia virus protooncogene (c-Mpl, or Mpl), controls HSC homeostasis and self-renewal. (jci.org)
  • Additionally, fibrosis has been induced in mice injected with high amounts of recombinant thrombopoietin. (nih.gov)
  • Product Description google The Biosensis Mouse Thrombopoietin/TPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. (biosensis.com)
  • Immunogen Description The standard and antigen for this mouse TPO ELISA is recombinant mouse TPO produced in a mammalian cell expression system and purified, aa.22-356 of mouse TPO. (biosensis.com)
  • Megakaryocytes :not detectable. (abcam.cn)
  • moreover, they also promote the hematopoietic differentiation of mouse ESCs and favor the development of definitive HSCs from pre-HSCs in the mouse aorta-gonad-mesonephros (AGM) region [ 8 ]. (biomedcentral.com)
  • IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. (rndsystems.com)
  • Recombinant Human IL-11 (Catalog # 218-IL ) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). (rndsystems.com)
  • Considering that TLR4 and TLR7 expression is not restricted to the nervous system and the gastrointestinal tube [ 15 , 16 ], in the present study we extended our investigation by evaluating the localization and appearance of these TLRs in developing respiratory system, liver and pancreas collected at different stages of mouse embryo development. (acbjournal.org)
  • Method for the quantitative measurement of collecting lymphatic vessel contraction in mice. (bu.edu)
  • ProxTom lymphatic vessel reporter mice reveal Prox1 expression in the adrenal medulla, megakaryocytes, and platelets. (bu.edu)
  • It is believed that megakaryocytes undergo a specialized form of apoptosis to shed platelets. (medhelp.org)
  • To resolve this paradox, we generated mice with hematopoietic- or megakaryocyte-specific deletions of the essential mediators of apoptosis, Bak and Bax. (medhelp.org)
  • We examined the effect on megakaryocytes of three agents that activate the intrinsic apoptosis pathway in other cell types: etoposide, staurosporine, and the BH3 mimetic ABT-737. (medhelp.org)
  • BALB/c mouse platelets were stained with anti-mouse CLEC-2 (CLEC1B) (clone 17D9/CLEC-2) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram). (biolegend.com)
  • Lymph node metastases can invade local blood vessels, exit the node, and colonize distant organs in mice. (bu.edu)
  • Amid the pandemic, the lab shifted its focus slightly and diverted some of their work to COVID-19 after several studies from across the country revealed that people who died from the coronavirus had a high number of megakaryocytes built up in various organs, causing significant issues. (iu.edu)
  • They then tested the cultured platelets by infusing them into immunodeficient mouse models and confirmed that they had the same life span as human platelets infused in mice. (ibtimes.com)
  • These changes were observed even in mice with mild and asymptomatic infections. (iu.edu)
  • Within the extracellular domain, mouse ITGA2b shares 81% and 89% amino acid sequence identity with human and rat ITGA2b, respectively. (rndsystems.com)
  • The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. (reliatech.de)
  • Megakaryocyte-associated tyrosine-protein kinase is an enzyme that in humans is encoded by the MATK gene. (wikipedia.org)
  • Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
  • Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. (rndsystems.com)
  • Deletion of Bak and Bax rendered megakaryocytes resistant to etoposide and ABT-737. (medhelp.org)
  • Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis. (ohri.ca)
  • The purpose of this kit is the in-vitro quantitative determination of mouse TPO in samples such as culture supernatant, tissue homogenates, plasma (EDTA) and plasma (Heparin). (biosensis.com)
  • In mice, knockout of the gene for factor XI does not lead to death in utero. (medscape.com)
  • Inhibition of YAP or TGF-β breaks this loop and suppresses cancer stemness and proliferation In nude mice, RAD18 promoted subcutaneous transplanted tumor growth and M2-type TAM recruitment. (nature.com)
  • These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. (nih.gov)
  • 6] In a mouse model, factor XI appears to play a greater role in thrombosis than in hemostasis. (medscape.com)
  • Nephropathy and Elevated BP in Mice with Podocyte-Specific NADPH Oxidase 5 Expression. (ohri.ca)
  • We have previously described TLR4 and TLR7 expression in developing mouse peripheral nervous system and gastrointestinal tract. (acbjournal.org)